## htsjdk.samtools.metrics.StringHeader # MarkDuplicates MAX_FILE_HANDLES_FOR_READ_ENDS_MAP=999 INPUT=[/scratch/tvdkreeke/GHE_RNAseq/GHE_exp2/results/star/GRCh38-45942_Exp215_GHEtest_6_S2_IPSC_noRibo_A02.samtools-coordinate-unsieved.bam] OUTPUT=/scratch/tvdkreeke/GHE_RNAseq/GHE_exp2/results/star/GRCh38-45942_Exp215_GHEtest_6_S2_IPSC_noRibo_A02.samtools-coordinate-dupmarked.bam METRICS_FILE=/scratch/tvdkreeke/GHE_RNAseq/GHE_exp2/results/qc/markdup/GRCh38-45942_Exp215_GHEtest_6_S2_IPSC_noRibo_A02.samtools-coordinate.metrics.txt TMP_DIR=[/tmp] MAX_SEQUENCES_FOR_DISK_READ_ENDS_MAP=50000 SORTING_COLLECTION_SIZE_RATIO=0.25 TAG_DUPLICATE_SET_MEMBERS=false REMOVE_SEQUENCING_DUPLICATES=false TAGGING_POLICY=DontTag CLEAR_DT=true DUPLEX_UMI=false FLOW_MODE=false FLOW_QUALITY_SUM_STRATEGY=false USE_END_IN_UNPAIRED_READS=false USE_UNPAIRED_CLIPPED_END=false UNPAIRED_END_UNCERTAINTY=0 FLOW_SKIP_FIRST_N_FLOWS=0 FLOW_Q_IS_KNOWN_END=false FLOW_EFFECTIVE_QUALITY_THRESHOLD=15 ADD_PG_TAG_TO_READS=true REMOVE_DUPLICATES=false ASSUME_SORTED=false DUPLICATE_SCORING_STRATEGY=SUM_OF_BASE_QUALITIES PROGRAM_RECORD_ID=MarkDuplicates PROGRAM_GROUP_NAME=MarkDuplicates READ_NAME_REGEX= OPTICAL_DUPLICATE_PIXEL_DISTANCE=100 MAX_OPTICAL_DUPLICATE_SET_SIZE=300000 VERBOSITY=INFO QUIET=false VALIDATION_STRINGENCY=STRICT COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false USE_JDK_DEFLATER=false USE_JDK_INFLATER=false ## htsjdk.samtools.metrics.StringHeader # Started on: Tue Sep 24 14:38:11 CEST 2024 ## METRICS CLASS picard.sam.DuplicationMetrics LIBRARY UNPAIRED_READS_EXAMINED READ_PAIRS_EXAMINED SECONDARY_OR_SUPPLEMENTARY_RDS UNMAPPED_READS UNPAIRED_READ_DUPLICATES READ_PAIR_DUPLICATES READ_PAIR_OPTICAL_DUPLICATES PERCENT_DUPLICATION ESTIMATED_LIBRARY_SIZE Unknown Library 0 2364756 1339400 0 0 477670 15468 0.201995 5158617 ## HISTOGRAM java.lang.Double BIN CoverageMult all_sets optical_sets non_optical_sets 1.0 1.00519 1551635 0 1561769 2.0 1.640761 269578 15013 262989 3.0 2.042626 47984 202 45334 4.0 2.29672 9745 10 9087 5.0 2.457381 2881 1 2753 6.0 2.558965 1298 2 1246 7.0 2.623196 808 0 792 8.0 2.663808 514 1 506 9.0 2.689487 410 0 408 10.0 2.705723 273 0 269 11.0 2.715989 290 0 281 12.0 2.72248 184 0 179 13.0 2.726584 147 0 147 14.0 2.729179 139 0 142 15.0 2.73082 103 0 94 16.0 2.731858 88 0 90 17.0 2.732514 96 0 93 18.0 2.732928 74 0 74 19.0 2.733191 56 0 54 20.0 2.733357 56 0 58 21.0 2.733461 45 0 46 22.0 2.733528 42 0 45 23.0 2.73357 41 0 43 24.0 2.733596 45 0 43 25.0 2.733613 39 0 33 26.0 2.733623 22 0 22 27.0 2.73363 29 0 32 28.0 2.733634 19 0 15 29.0 2.733637 13 0 14 30.0 2.733639 25 0 31 31.0 2.73364 19 0 15 32.0 2.733641 18 0 15 33.0 2.733641 13 0 13 34.0 2.733641 19 0 17 35.0 2.733641 11 0 16 36.0 2.733642 18 0 16 37.0 2.733642 18 0 17 38.0 2.733642 15 0 12 39.0 2.733642 3 0 6 40.0 2.733642 10 0 11 41.0 2.733642 11 0 9 42.0 2.733642 7 0 11 43.0 2.733642 10 0 8 44.0 2.733642 12 0 11 45.0 2.733642 8 0 7 46.0 2.733642 7 0 9 47.0 2.733642 13 0 14 48.0 2.733642 8 0 5 49.0 2.733642 6 0 6 50.0 2.733642 7 0 9 51.0 2.733642 9 0 7 52.0 2.733642 4 0 3 53.0 2.733642 2 0 3 54.0 2.733642 5 0 4 55.0 2.733642 2 0 5 56.0 2.733642 5 0 2 57.0 2.733642 4 0 6 58.0 2.733642 8 0 7 59.0 2.733642 3 0 4 60.0 2.733642 6 0 3 61.0 2.733642 4 0 6 62.0 2.733642 6 0 5 63.0 2.733642 1 0 0 64.0 2.733642 5 0 7 65.0 2.733642 3 0 2 66.0 2.733642 1 0 4 67.0 2.733642 4 0 1 68.0 2.733642 3 0 5 69.0 2.733642 7 0 4 70.0 2.733642 2 0 3 71.0 2.733642 3 0 2 72.0 2.733642 2 0 3 73.0 2.733642 1 0 2 74.0 2.733642 3 0 2 75.0 2.733642 2 0 3 76.0 2.733642 2 0 2 77.0 2.733642 1 0 0 78.0 2.733642 2 0 3 79.0 2.733642 2 0 2 80.0 2.733642 4 0 3 81.0 2.733642 1 0 1 82.0 2.733642 3 0 3 83.0 2.733642 1 0 1 84.0 2.733642 2 0 2 85.0 2.733642 1 0 0 86.0 2.733642 2 0 2 87.0 2.733642 0 0 0 88.0 2.733642 0 0 0 89.0 2.733642 0 0 0 90.0 2.733642 0 0 0 91.0 2.733642 1 0 3 92.0 2.733642 2 0 2 93.0 2.733642 1 0 0 94.0 2.733642 1 0 0 95.0 2.733642 0 0 0 96.0 2.733642 1 0 1 97.0 2.733642 1 0 1 98.0 2.733642 0 0 0 99.0 2.733642 0 0 0 100.0 2.733642 0 0 1 101.0 0 2 0 1 102.0 0 0 0 1 103.0 0 1 0 0 104.0 0 0 0 1 105.0 0 1 0 3 106.0 0 1 0 0 107.0 0 2 0 0 108.0 0 1 0 1 111.0 0 1 0 1 112.0 0 1 0 1 113.0 0 1 0 1 114.0 0 1 0 1 115.0 0 1 0 1 119.0 0 1 0 1 120.0 0 0 0 1 121.0 0 1 0 0 125.0 0 0 0 2 126.0 0 1 0 0 127.0 0 1 0 0 128.0 0 1 0 1 133.0 0 0 0 1 134.0 0 1 0 1 135.0 0 1 0 0 136.0 0 0 0 1 137.0 0 1 0 0 144.0 0 0 0 1 145.0 0 1 0 0 148.0 0 1 0 1 150.0 0 1 0 1 152.0 0 0 0 1 153.0 0 1 0 0 167.0 0 0 0 1 168.0 0 1 0 1 169.0 0 1 0 0 173.0 0 1 0 1 176.0 0 0 0 2 177.0 0 2 0 0 187.0 0 0 0 1 189.0 0 1 0 1 190.0 0 1 0 1 191.0 0 1 0 0 204.0 0 1 0 1 219.0 0 1 0 1 220.0 0 0 0 1 222.0 0 1 0 0 235.0 0 1 0 2 238.0 0 1 0 0 244.0 0 0 0 1 248.0 0 1 0 0 257.0 0 0 0 1 259.0 0 1 0 0 286.0 0 0 0 1 287.0 0 2 0 1 299.0 0 0 0 1 302.0 0 1 0 0 314.0 0 0 0 1 315.0 0 1 0 0 319.0 0 0 0 1 322.0 0 1 0 0 325.0 0 0 0 1 327.0 0 1 0 0 339.0 0 0 0 1 344.0 0 1 0 0 414.0 0 0 0 1 419.0 0 1 0 0 495.0 0 0 0 1 497.0 0 1 0 1 503.0 0 0 0 1 504.0 0 2 0 0