## htsjdk.samtools.metrics.StringHeader # MarkDuplicates MAX_FILE_HANDLES_FOR_READ_ENDS_MAP=999 INPUT=[/scratch/tdewijs/results/star/GRCg6a-GSM1481262.samtools-coordinate-unsieved.bam] OUTPUT=/scratch/tdewijs/results/star/GRCg6a-GSM1481262.samtools-coordinate-dupmarked.bam METRICS_FILE=/scratch/tdewijs/results/qc/markdup/GRCg6a-GSM1481262.samtools-coordinate.metrics.txt TMP_DIR=[/scratch/tdewijs/tmp] MAX_SEQUENCES_FOR_DISK_READ_ENDS_MAP=50000 SORTING_COLLECTION_SIZE_RATIO=0.25 TAG_DUPLICATE_SET_MEMBERS=false REMOVE_SEQUENCING_DUPLICATES=false TAGGING_POLICY=DontTag CLEAR_DT=true DUPLEX_UMI=false ADD_PG_TAG_TO_READS=true REMOVE_DUPLICATES=false ASSUME_SORTED=false DUPLICATE_SCORING_STRATEGY=SUM_OF_BASE_QUALITIES PROGRAM_RECORD_ID=MarkDuplicates PROGRAM_GROUP_NAME=MarkDuplicates READ_NAME_REGEX= OPTICAL_DUPLICATE_PIXEL_DISTANCE=100 MAX_OPTICAL_DUPLICATE_SET_SIZE=300000 VERBOSITY=INFO QUIET=false VALIDATION_STRINGENCY=STRICT COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false GA4GH_CLIENT_SECRETS=client_secrets.json USE_JDK_DEFLATER=false USE_JDK_INFLATER=false ## htsjdk.samtools.metrics.StringHeader # Started on: Thu Dec 09 17:37:56 CET 2021 ## METRICS CLASS picard.sam.DuplicationMetrics LIBRARY UNPAIRED_READS_EXAMINED READ_PAIRS_EXAMINED SECONDARY_OR_SUPPLEMENTARY_RDS UNMAPPED_READS UNPAIRED_READ_DUPLICATES READ_PAIR_DUPLICATES READ_PAIR_OPTICAL_DUPLICATES PERCENT_DUPLICATION ESTIMATED_LIBRARY_SIZE Unknown Library 0 19973054 1480636 0 0 3493706 0 0.174921 50219336 ## HISTOGRAM java.lang.Double BIN CoverageMult all_sets non_optical_sets 1.0 1 14927853 14927853 2.0 1.671853 1003917 1003917 3.0 2.123238 250169 250169 4.0 2.426503 105981 105981 5.0 2.630252 56401 56401 6.0 2.767142 34219 34219 7.0 2.859111 22191 22191 8.0 2.920901 15608 15608 9.0 2.962415 11269 11269 10.0 2.990306 8516 8516 11.0 3.009045 6545 6545 12.0 3.021634 5062 5062 13.0 3.030093 4174 4174 14.0 3.035775 3302 3302 15.0 3.039593 2853 2853 16.0 3.042158 2369 2369 17.0 3.043882 1994 1994 18.0 3.04504 1698 1698 19.0 3.045818 1470 1470 20.0 3.04634 1246 1246 21.0 3.046691 1092 1092 22.0 3.046927 1028 1028 23.0 3.047086 876 876 24.0 3.047192 783 783 25.0 3.047264 627 627 26.0 3.047312 611 611 27.0 3.047344 497 497 28.0 3.047366 485 485 29.0 3.047381 410 410 30.0 3.04739 406 406 31.0 3.047397 333 333 32.0 3.047401 350 350 33.0 3.047404 288 288 34.0 3.047406 289 289 35.0 3.047408 243 243 36.0 3.047409 224 224 37.0 3.047409 242 242 38.0 3.04741 209 209 39.0 3.04741 180 180 40.0 3.04741 147 147 41.0 3.04741 183 183 42.0 3.04741 147 147 43.0 3.04741 130 130 44.0 3.04741 140 140 45.0 3.04741 131 131 46.0 3.04741 107 107 47.0 3.04741 94 94 48.0 3.04741 105 105 49.0 3.04741 89 89 50.0 3.04741 82 82 51.0 3.04741 89 89 52.0 3.04741 93 93 53.0 3.04741 68 68 54.0 3.04741 67 67 55.0 3.04741 63 63 56.0 3.04741 78 78 57.0 3.04741 65 65 58.0 3.04741 54 54 59.0 3.04741 51 51 60.0 3.04741 50 50 61.0 3.04741 62 62 62.0 3.04741 40 40 63.0 3.04741 39 39 64.0 3.04741 40 40 65.0 3.04741 29 29 66.0 3.04741 44 44 67.0 3.04741 30 30 68.0 3.04741 30 30 69.0 3.04741 43 43 70.0 3.04741 24 24 71.0 3.04741 35 35 72.0 3.04741 34 34 73.0 3.04741 27 27 74.0 3.04741 29 29 75.0 3.04741 22 22 76.0 3.04741 31 31 77.0 3.04741 28 28 78.0 3.04741 26 26 79.0 3.04741 19 19 80.0 3.04741 21 21 81.0 3.04741 23 23 82.0 3.04741 15 15 83.0 3.04741 14 14 84.0 3.04741 25 25 85.0 3.04741 15 15 86.0 3.04741 17 17 87.0 3.04741 11 11 88.0 3.04741 15 15 89.0 3.04741 19 19 90.0 3.04741 12 12 91.0 3.04741 9 9 92.0 3.04741 17 17 93.0 3.04741 10 10 94.0 3.04741 13 13 95.0 3.04741 12 12 96.0 3.04741 21 21 97.0 3.04741 9 9 98.0 3.04741 8 8 99.0 3.04741 8 8 100.0 3.04741 11 11 101.0 0 6 6 102.0 0 6 6 103.0 0 8 8 104.0 0 10 10 105.0 0 9 9 106.0 0 11 11 107.0 0 6 6 108.0 0 11 11 109.0 0 5 5 110.0 0 6 6 111.0 0 6 6 112.0 0 4 4 113.0 0 3 3 114.0 0 6 6 115.0 0 2 2 116.0 0 7 7 117.0 0 5 5 118.0 0 4 4 119.0 0 7 7 120.0 0 3 3 121.0 0 6 6 122.0 0 5 5 123.0 0 5 5 124.0 0 2 2 125.0 0 1 1 126.0 0 5 5 127.0 0 5 5 128.0 0 7 7 129.0 0 8 8 130.0 0 2 2 131.0 0 4 4 132.0 0 3 3 133.0 0 2 2 134.0 0 2 2 135.0 0 8 8 136.0 0 4 4 137.0 0 6 6 138.0 0 6 6 139.0 0 2 2 140.0 0 2 2 141.0 0 1 1 142.0 0 5 5 143.0 0 6 6 144.0 0 2 2 145.0 0 1 1 146.0 0 4 4 147.0 0 3 3 148.0 0 4 4 149.0 0 2 2 150.0 0 2 2 151.0 0 3 3 152.0 0 3 3 154.0 0 2 2 156.0 0 2 2 157.0 0 5 5 158.0 0 5 5 163.0 0 1 1 165.0 0 2 2 166.0 0 2 2 168.0 0 1 1 169.0 0 1 1 170.0 0 3 3 172.0 0 1 1 174.0 0 3 3 175.0 0 3 3 176.0 0 2 2 177.0 0 2 2 178.0 0 3 3 179.0 0 2 2 180.0 0 4 4 181.0 0 1 1 182.0 0 1 1 183.0 0 2 2 187.0 0 1 1 189.0 0 1 1 194.0 0 1 1 201.0 0 2 2 202.0 0 1 1 203.0 0 1 1 206.0 0 1 1 207.0 0 1 1 212.0 0 1 1 214.0 0 1 1 215.0 0 1 1 216.0 0 1 1 218.0 0 1 1 219.0 0 1 1 223.0 0 1 1 229.0 0 1 1 230.0 0 1 1 234.0 0 1 1 237.0 0 1 1 239.0 0 1 1 240.0 0 1 1 243.0 0 2 2 244.0 0 2 2 246.0 0 3 3 249.0 0 1 1 252.0 0 1 1 255.0 0 1 1 258.0 0 1 1 260.0 0 1 1 266.0 0 1 1 268.0 0 1 1 269.0 0 1 1 270.0 0 3 3 271.0 0 1 1 275.0 0 1 1 277.0 0 1 1 278.0 0 1 1 280.0 0 2 2 281.0 0 2 2 288.0 0 1 1 296.0 0 2 2 297.0 0 1 1 323.0 0 1 1 326.0 0 1 1 329.0 0 1 1 349.0 0 1 1 350.0 0 3 3 357.0 0 1 1 368.0 0 1 1 369.0 0 1 1 372.0 0 1 1 385.0 0 1 1 436.0 0 1 1 549.0 0 1 1 552.0 0 1 1 577.0 0 1 1 636.0 0 1 1 652.0 0 1 1 664.0 0 1 1 668.0 0 1 1 681.0 0 1 1 777.0 0 1 1