## htsjdk.samtools.metrics.StringHeader # MarkDuplicates MAX_FILE_HANDLES_FOR_READ_ENDS_MAP=999 INPUT=[/ceph/rimlsfnwi/data/moldevbio/heeringen/siebrenf/xt_rna/results/star/Xenopus_tropicalis_v9.1-GSM1606361.samtools-coordinate-unsieved.bam] OUTPUT=/ceph/rimlsfnwi/data/moldevbio/heeringen/siebrenf/xt_rna/results/star/Xenopus_tropicalis_v9.1-GSM1606361.samtools-coordinate-dupmarked.bam METRICS_FILE=/ceph/rimlsfnwi/data/moldevbio/heeringen/siebrenf/xt_rna/results/qc/markdup/Xenopus_tropicalis_v9.1-GSM1606361.samtools-coordinate.metrics.txt TMP_DIR=[/scratch/siebrenf/tmp] MAX_SEQUENCES_FOR_DISK_READ_ENDS_MAP=50000 SORTING_COLLECTION_SIZE_RATIO=0.25 TAG_DUPLICATE_SET_MEMBERS=false REMOVE_SEQUENCING_DUPLICATES=false TAGGING_POLICY=DontTag CLEAR_DT=true DUPLEX_UMI=false ADD_PG_TAG_TO_READS=true REMOVE_DUPLICATES=false ASSUME_SORTED=false DUPLICATE_SCORING_STRATEGY=SUM_OF_BASE_QUALITIES PROGRAM_RECORD_ID=MarkDuplicates PROGRAM_GROUP_NAME=MarkDuplicates READ_NAME_REGEX= OPTICAL_DUPLICATE_PIXEL_DISTANCE=100 MAX_OPTICAL_DUPLICATE_SET_SIZE=300000 VERBOSITY=INFO QUIET=false VALIDATION_STRINGENCY=STRICT COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false GA4GH_CLIENT_SECRETS=client_secrets.json USE_JDK_DEFLATER=false USE_JDK_INFLATER=false ## htsjdk.samtools.metrics.StringHeader # Started on: Tue Nov 02 14:41:03 CET 2021 ## METRICS CLASS picard.sam.DuplicationMetrics LIBRARY UNPAIRED_READS_EXAMINED READ_PAIRS_EXAMINED SECONDARY_OR_SUPPLEMENTARY_RDS UNMAPPED_READS UNPAIRED_READ_DUPLICATES READ_PAIR_DUPLICATES READ_PAIR_OPTICAL_DUPLICATES PERCENT_DUPLICATION ESTIMATED_LIBRARY_SIZE Unknown Library 0 24978731 3545588 0 0 4121642 0 0.165006 67112442 ## HISTOGRAM java.lang.Double BIN CoverageMult all_sets non_optical_sets 1.0 1 18837594 18837594 2.0 1.689222 1366512 1366512 3.0 2.164248 312198 312198 4.0 2.491647 127292 127292 5.0 2.717297 66169 66169 6.0 2.87282 39035 39035 7.0 2.98001 25082 25082 8.0 3.053888 17400 17400 9.0 3.104806 12288 12288 10.0 3.1399 9400 9400 11.0 3.164087 7125 7125 12.0 3.180758 5524 5524 13.0 3.192247 4399 4399 14.0 3.200166 3490 3490 15.0 3.205624 2874 2874 16.0 3.209386 2466 2466 17.0 3.211978 2172 2172 18.0 3.213765 1711 1711 19.0 3.214997 1524 1524 20.0 3.215846 1292 1292 21.0 3.216431 1042 1042 22.0 3.216834 1019 1019 23.0 3.217112 847 847 24.0 3.217303 754 754 25.0 3.217435 682 682 26.0 3.217526 547 547 27.0 3.217589 511 511 28.0 3.217632 498 498 29.0 3.217662 445 445 30.0 3.217683 369 369 31.0 3.217697 343 343 32.0 3.217707 284 284 33.0 3.217713 270 270 34.0 3.217718 246 246 35.0 3.217721 230 230 36.0 3.217723 201 201 37.0 3.217725 190 190 38.0 3.217726 156 156 39.0 3.217727 156 156 40.0 3.217727 175 175 41.0 3.217727 126 126 42.0 3.217728 130 130 43.0 3.217728 117 117 44.0 3.217728 135 135 45.0 3.217728 93 93 46.0 3.217728 101 101 47.0 3.217728 82 82 48.0 3.217728 98 98 49.0 3.217728 72 72 50.0 3.217728 76 76 51.0 3.217728 62 62 52.0 3.217728 65 65 53.0 3.217728 55 55 54.0 3.217728 52 52 55.0 3.217728 54 54 56.0 3.217728 55 55 57.0 3.217728 49 49 58.0 3.217728 49 49 59.0 3.217728 56 56 60.0 3.217728 49 49 61.0 3.217728 40 40 62.0 3.217728 34 34 63.0 3.217728 36 36 64.0 3.217728 37 37 65.0 3.217728 29 29 66.0 3.217728 34 34 67.0 3.217728 27 27 68.0 3.217728 28 28 69.0 3.217728 29 29 70.0 3.217728 27 27 71.0 3.217728 22 22 72.0 3.217728 30 30 73.0 3.217728 23 23 74.0 3.217728 23 23 75.0 3.217728 21 21 76.0 3.217728 20 20 77.0 3.217728 14 14 78.0 3.217728 13 13 79.0 3.217728 13 13 80.0 3.217728 13 13 81.0 3.217728 14 14 82.0 3.217728 15 15 83.0 3.217728 18 18 84.0 3.217728 13 13 85.0 3.217728 14 14 86.0 3.217728 13 13 87.0 3.217728 10 10 88.0 3.217728 16 16 89.0 3.217728 12 12 90.0 3.217728 8 8 91.0 3.217728 7 7 92.0 3.217728 15 15 93.0 3.217728 8 8 94.0 3.217728 14 14 95.0 3.217728 11 11 96.0 3.217728 7 7 97.0 3.217728 4 4 98.0 3.217728 6 6 99.0 3.217728 9 9 100.0 3.217728 6 6 101.0 0 6 6 102.0 0 12 12 103.0 0 6 6 104.0 0 8 8 105.0 0 10 10 106.0 0 7 7 107.0 0 8 8 108.0 0 3 3 109.0 0 6 6 110.0 0 5 5 111.0 0 3 3 112.0 0 2 2 113.0 0 8 8 114.0 0 8 8 115.0 0 5 5 116.0 0 4 4 117.0 0 3 3 118.0 0 5 5 119.0 0 1 1 120.0 0 5 5 121.0 0 3 3 122.0 0 3 3 123.0 0 3 3 124.0 0 1 1 125.0 0 5 5 126.0 0 3 3 127.0 0 3 3 128.0 0 4 4 129.0 0 4 4 130.0 0 2 2 131.0 0 3 3 132.0 0 3 3 133.0 0 3 3 134.0 0 7 7 135.0 0 3 3 136.0 0 3 3 137.0 0 2 2 138.0 0 3 3 140.0 0 1 1 142.0 0 3 3 143.0 0 1 1 144.0 0 4 4 145.0 0 1 1 146.0 0 1 1 147.0 0 1 1 148.0 0 2 2 150.0 0 1 1 151.0 0 1 1 154.0 0 1 1 155.0 0 1 1 157.0 0 2 2 158.0 0 2 2 159.0 0 1 1 162.0 0 1 1 163.0 0 1 1 164.0 0 2 2 165.0 0 2 2 166.0 0 1 1 167.0 0 3 3 168.0 0 1 1 169.0 0 1 1 170.0 0 1 1 171.0 0 1 1 173.0 0 2 2 174.0 0 1 1 176.0 0 1 1 178.0 0 1 1 180.0 0 1 1 182.0 0 1 1 183.0 0 1 1 185.0 0 2 2 188.0 0 1 1 190.0 0 2 2 192.0 0 2 2 194.0 0 1 1 195.0 0 2 2 203.0 0 1 1 204.0 0 1 1 206.0 0 1 1 217.0 0 1 1 220.0 0 3 3 222.0 0 2 2 227.0 0 1 1 228.0 0 1 1 229.0 0 1 1 230.0 0 1 1 232.0 0 1 1 233.0 0 2 2 239.0 0 1 1 240.0 0 1 1 245.0 0 1 1 249.0 0 1 1 253.0 0 1 1 259.0 0 2 2 260.0 0 1 1 262.0 0 1 1 268.0 0 1 1 278.0 0 1 1 288.0 0 1 1 290.0 0 1 1 305.0 0 1 1 307.0 0 1 1 327.0 0 1 1 346.0 0 1 1 367.0 0 1 1 377.0 0 1 1 452.0 0 1 1 456.0 0 1 1 462.0 0 1 1 556.0 0 1 1 7570.0 0 1 1