## htsjdk.samtools.metrics.StringHeader # MarkDuplicates MAX_FILE_HANDLES_FOR_READ_ENDS_MAP=999 INPUT=[/ceph/rimlsfnwi/data/moldevbio/heeringen/siebrenf/xt_rna/results/star/Xenopus_tropicalis_v9.1-GSM1606222.samtools-coordinate-unsieved.bam] OUTPUT=/ceph/rimlsfnwi/data/moldevbio/heeringen/siebrenf/xt_rna/results/star/Xenopus_tropicalis_v9.1-GSM1606222.samtools-coordinate-dupmarked.bam METRICS_FILE=/ceph/rimlsfnwi/data/moldevbio/heeringen/siebrenf/xt_rna/results/qc/markdup/Xenopus_tropicalis_v9.1-GSM1606222.samtools-coordinate.metrics.txt TMP_DIR=[/scratch/siebrenf/tmp] MAX_SEQUENCES_FOR_DISK_READ_ENDS_MAP=50000 SORTING_COLLECTION_SIZE_RATIO=0.25 TAG_DUPLICATE_SET_MEMBERS=false REMOVE_SEQUENCING_DUPLICATES=false TAGGING_POLICY=DontTag CLEAR_DT=true DUPLEX_UMI=false ADD_PG_TAG_TO_READS=true REMOVE_DUPLICATES=false ASSUME_SORTED=false DUPLICATE_SCORING_STRATEGY=SUM_OF_BASE_QUALITIES PROGRAM_RECORD_ID=MarkDuplicates PROGRAM_GROUP_NAME=MarkDuplicates READ_NAME_REGEX= OPTICAL_DUPLICATE_PIXEL_DISTANCE=100 MAX_OPTICAL_DUPLICATE_SET_SIZE=300000 VERBOSITY=INFO QUIET=false VALIDATION_STRINGENCY=STRICT COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false GA4GH_CLIENT_SECRETS=client_secrets.json USE_JDK_DEFLATER=false USE_JDK_INFLATER=false ## htsjdk.samtools.metrics.StringHeader # Started on: Tue Nov 02 00:02:15 CET 2021 ## METRICS CLASS picard.sam.DuplicationMetrics LIBRARY UNPAIRED_READS_EXAMINED READ_PAIRS_EXAMINED SECONDARY_OR_SUPPLEMENTARY_RDS UNMAPPED_READS UNPAIRED_READ_DUPLICATES READ_PAIR_DUPLICATES READ_PAIR_OPTICAL_DUPLICATES PERCENT_DUPLICATION ESTIMATED_LIBRARY_SIZE Unknown Library 0 36503760 5619770 0 0 5051879 0 0.138393 119412621 ## HISTOGRAM java.lang.Double BIN CoverageMult all_sets non_optical_sets 1.0 1 28854344 28854344 2.0 1.736612 1784693 1784693 3.0 2.279209 400383 400383 4.0 2.678892 159044 159044 5.0 2.973303 80924 80924 6.0 3.19017 47107 47107 7.0 3.349917 30486 30486 8.0 3.467588 20676 20676 9.0 3.554266 14657 14657 10.0 3.618114 10802 10802 11.0 3.665145 8280 8280 12.0 3.699789 6195 6195 13.0 3.725308 4951 4951 14.0 3.744106 4043 4043 15.0 3.757952 3240 3240 16.0 3.768152 2655 2655 17.0 3.775665 2276 2276 18.0 3.781199 1875 1875 19.0 3.785276 1638 1638 20.0 3.788279 1392 1392 21.0 3.790491 1285 1285 22.0 3.79212 1066 1066 23.0 3.79332 902 902 24.0 3.794204 815 815 25.0 3.794855 687 687 26.0 3.795335 600 600 27.0 3.795688 562 562 28.0 3.795949 482 482 29.0 3.79614 434 434 30.0 3.796282 417 417 31.0 3.796386 330 330 32.0 3.796462 289 289 33.0 3.796519 296 296 34.0 3.79656 296 296 35.0 3.796591 260 260 36.0 3.796614 213 213 37.0 3.79663 199 199 38.0 3.796642 205 205 39.0 3.796651 163 163 40.0 3.796658 144 144 41.0 3.796663 158 158 42.0 3.796667 124 124 43.0 3.796669 126 126 44.0 3.796671 126 126 45.0 3.796673 105 105 46.0 3.796674 100 100 47.0 3.796674 82 82 48.0 3.796675 109 109 49.0 3.796675 93 93 50.0 3.796676 63 63 51.0 3.796676 84 84 52.0 3.796676 64 64 53.0 3.796676 65 65 54.0 3.796676 52 52 55.0 3.796676 54 54 56.0 3.796676 69 69 57.0 3.796677 46 46 58.0 3.796677 52 52 59.0 3.796677 47 47 60.0 3.796677 36 36 61.0 3.796677 38 38 62.0 3.796677 39 39 63.0 3.796677 35 35 64.0 3.796677 38 38 65.0 3.796677 35 35 66.0 3.796677 31 31 67.0 3.796677 19 19 68.0 3.796677 26 26 69.0 3.796677 20 20 70.0 3.796677 32 32 71.0 3.796677 20 20 72.0 3.796677 18 18 73.0 3.796677 17 17 74.0 3.796677 19 19 75.0 3.796677 22 22 76.0 3.796677 16 16 77.0 3.796677 13 13 78.0 3.796677 22 22 79.0 3.796677 14 14 80.0 3.796677 16 16 81.0 3.796677 13 13 82.0 3.796677 15 15 83.0 3.796677 12 12 84.0 3.796677 18 18 85.0 3.796677 9 9 86.0 3.796677 16 16 87.0 3.796677 8 8 88.0 3.796677 11 11 89.0 3.796677 15 15 90.0 3.796677 11 11 91.0 3.796677 8 8 92.0 3.796677 8 8 93.0 3.796677 11 11 94.0 3.796677 9 9 95.0 3.796677 4 4 96.0 3.796677 4 4 97.0 3.796677 9 9 98.0 3.796677 7 7 99.0 3.796677 3 3 100.0 3.796677 5 5 101.0 0 7 7 102.0 0 7 7 103.0 0 8 8 104.0 0 7 7 105.0 0 5 5 106.0 0 6 6 107.0 0 3 3 108.0 0 5 5 109.0 0 8 8 110.0 0 2 2 111.0 0 6 6 112.0 0 2 2 113.0 0 5 5 114.0 0 3 3 115.0 0 3 3 116.0 0 1 1 117.0 0 3 3 118.0 0 7 7 119.0 0 5 5 120.0 0 2 2 121.0 0 3 3 122.0 0 7 7 123.0 0 4 4 124.0 0 2 2 125.0 0 2 2 126.0 0 4 4 127.0 0 2 2 128.0 0 1 1 129.0 0 3 3 131.0 0 1 1 132.0 0 1 1 133.0 0 5 5 134.0 0 3 3 135.0 0 1 1 136.0 0 1 1 137.0 0 1 1 139.0 0 2 2 141.0 0 1 1 142.0 0 1 1 143.0 0 2 2 144.0 0 5 5 145.0 0 4 4 146.0 0 4 4 147.0 0 1 1 148.0 0 1 1 150.0 0 5 5 151.0 0 1 1 152.0 0 2 2 153.0 0 2 2 154.0 0 2 2 155.0 0 2 2 156.0 0 2 2 157.0 0 2 2 158.0 0 1 1 159.0 0 2 2 160.0 0 1 1 161.0 0 1 1 162.0 0 1 1 163.0 0 1 1 164.0 0 1 1 165.0 0 1 1 166.0 0 2 2 167.0 0 2 2 168.0 0 1 1 169.0 0 1 1 170.0 0 1 1 171.0 0 1 1 173.0 0 1 1 175.0 0 3 3 177.0 0 1 1 178.0 0 1 1 180.0 0 3 3 181.0 0 2 2 184.0 0 2 2 190.0 0 1 1 191.0 0 2 2 192.0 0 1 1 198.0 0 2 2 201.0 0 1 1 202.0 0 1 1 206.0 0 1 1 207.0 0 1 1 214.0 0 1 1 215.0 0 1 1 222.0 0 1 1 223.0 0 1 1 227.0 0 1 1 235.0 0 2 2 238.0 0 1 1 244.0 0 1 1 255.0 0 1 1 285.0 0 1 1 306.0 0 2 2 327.0 0 1 1 330.0 0 1 1 343.0 0 1 1 368.0 0 1 1 476.0 0 1 1 2603.0 0 1 1