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"CGATT":196549, "CGATC":522296, "CGATG":280485, "CGACA":249154, "CGACT":183803, "CGACC":529601, "CGACG":456855, "CGAGA":390350, "CGAGT":228106, "CGAGC":409553, "CGAGG":724468, "CGTAA":127301, "CGTAT":145892, "CGTAC":145243, "CGTAG":181787, "CGTTA":98224, "CGTTT":418659, "CGTTC":455203, "CGTTG":203563, "CGTCA":253040, "CGTCT":453786, "CGTCC":380089, "CGTCG":202341, "CGTGA":306668, "CGTGT":268940, "CGTGC":366576, "CGTGG":393760, "CGCAA":176413, "CGCAT":151937, "CGCAC":411289, "CGCAG":464613, "CGCTA":307213, "CGCTT":288539, "CGCTC":746474, "CGCTG":389458, "CGCCA":464046, "CGCCT":552864, "CGCCC":703353, "CGCCG":609787, "CGCGA":507341, "CGCGT":249709, "CGCGC":611676, "CGCGG":787854, "CGGAA":297671, "CGGAT":221985, "CGGAC":438570, "CGGAG":664051, "CGGTA":175741, "CGGTT":404936, "CGGTC":367383, "CGGTG":402235, "CGGCA":463973, "CGGCT":428315, "CGGCC":1062833, "CGGCG":950011, "CGGGA":934962, "CGGGT":368837, "CGGGC":1017396, "CGGGG":1305712, "GAAAA":1366121, "GAAAT":905394, "GAAAC":779166, 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"GTAAT":580705, "GTAAC":368032, "GTAAG":466766, "GTATA":378664, "GTATT":572697, "GTATC":331163, "GTATG":371416, "GTACA":503453, "GTACT":443465, "GTACC":324447, "GTACG":153189, "GTAGA":616440, "GTAGT":502360, "GTAGC":515002, "GTAGG":559533, "GTTAA":477240, "GTTAT":420252, "GTTAC":332908, "GTTAG":352227, "GTTTA":555514, "GTTTT":1245308, "GTTTC":1086841, "GTTTG":790439, "GTTCA":1019512, "GTTCT":1110318, "GTTCC":692863, "GTTCG":201628, "GTTGA":558437, "GTTGT":604980, "GTTGC":692343, "GTTGG":713119, "GTCAA":579881, "GTCAT":613116, "GTCAC":830876, "GTCAG":853100, "GTCTA":350437, "GTCTT":997881, "GTCTC":1131544, "GTCTG":918139, "GTCCA":828186, "GTCCT":874732, "GTCCC":986672, "GTCCG":247209, "GTCGA":194344, "GTCGT":242031, "GTCGC":265466, "GTCGG":303525, "GTGAA":774181, "GTGAT":720243, "GTGAC":554999, "GTGAG":888512, "GTGTA":443515, "GTGTT":632694, "GTGTC":621154, "GTGTG":904989, "GTGCA":1051921, "GTGCT":771935, "GTGCC":824747, "GTGCG":281588, "GTGGA":798577, "GTGGT":1131106, "GTGGC":1406363, 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"GCGGA":552856, "GCGGT":307910, "GCGGC":997652, "GCGGG":1108242, "GGAAA":1159643, "GGAAT":711192, "GGAAC":643737, "GGAAG":1560549, "GGATA":431380, "GGATT":645639, "GGATC":592218, "GGATG":909992, "GGACA":861099, "GGACT":629900, "GGACC":718261, "GGACG":576852, "GGAGA":1404377, "GGAGT":1408294, "GGAGC":973927, "GGAGG":2264479, "GGTAA":486608, "GGTAT":400750, "GGTAC":424780, "GGTAG":581948, "GGTTA":401120, "GGTTT":839280, "GGTTC":953742, "GGTTG":651159, "GGTCA":929414, "GGTCT":905466, "GGTCC":942472, "GGTCG":332403, "GGTGA":892407, "GGTGT":726331, "GGTGC":772721, "GGTGG":1641715, "GGCAA":1059607, "GGCAT":836314, "GGCAC":930184, "GGCAG":1641602, "GGCTA":852497, "GGCTT":846387, "GGCTC":1045385, "GGCTG":1883129, "GGCCA":1195355, "GGCCT":1037883, "GGCCC":1300757, "GGCCG":1191896, "GGCGA":517812, "GGCGT":343216, "GGCGC":1111089, "GGCGG":1394979, "GGGAA":1222335, "GGGAT":763393, "GGGAC":954771, "GGGAG":2089827, "GGGTA":452552, "GGGTT":761074, "GGGTC":796625, "GGGTG":1096068, "GGGCA":1182837, "GGGCT":1023499, "GGGCC":1501758, "GGGCG":1004677, "GGGGA":1545575, "GGGGT":980795, "GGGGC":1357261, "GGGGG":1518330 }, "overrepresented_sequences": { } }, "read2_before_filtering": { "total_reads": 20644498, "total_bases": 887713414, "q20_bases": 854059870, "q30_bases": 844398785, "total_cycles": 43, "quality_curves": { "A":[31.1698,31.0272,31.2357,31.2369,31.2774,34.8267,34.82,34.7986,34.8172,34.7727,34.8354,34.8635,34.8205,34.8109,34.8254,34.8097,34.7835,34.7716,34.7803,34.751,34.7388,34.7121,34.6985,34.7011,34.6653,34.6653,34.6512,34.6569,34.6339,34.6143,34.6016,34.5773,34.5694,34.567,34.525,34.5268,34.5212,34.4884,34.4773,34.4579,34.4244,34.4142,33.7255], "T":[31.4996,31.3764,31.4279,31.4547,31.4632,35.1655,35.1755,35.1514,35.1602,35.177,35.1427,35.1782,35.1728,35.166,35.1666,35.1565,35.1264,35.1441,35.1414,35.1258,35.1243,35.1165,35.1,35.1008,35.094,35.0734,35.0668,35.0707,35.0576,35.0534,35.0442,35.036,35.0191,35.0051,34.9933,35.0081,34.9854,34.9566,34.9455,34.9387,34.9246,34.8936,34.6309], "C":[31.5437,31.4155,31.4517,31.4844,31.4949,35.1312,35.0364,35.0477,35.0426,35.0609,35.1049,35.0897,35.0046,35.0231,35.0461,35.0253,35.0181,34.9962,34.9949,34.9875,34.9206,34.8913,34.8917,34.9105,34.8928,34.8442,34.8566,34.861,34.8422,34.8371,34.8059,34.7868,34.7561,34.754,34.7421,34.7366,34.7434,34.6867,34.6833,34.6554,34.6458,34.5903,34.0772], 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"C":[0.360251,0.217685,0.233011,0.232331,0.200494,0.227864,0.238122,0.303951,0.27811,0.221127,0.28556,0.273136,0.259857,0.246623,0.244906,0.252406,0.254524,0.249073,0.246273,0.253463,0.25393,0.256139,0.251303,0.245029,0.25545,0.248865,0.245247,0.248987,0.248284,0.251026,0.250754,0.254673,0.254258,0.252242,0.251029,0.249732,0.257295,0.256762,0.252073,0.253616,0.253569,0.256527,0.255048], "G":[0.345696,0.263174,0.24532,0.29268,0.3062,0.245342,0.237205,0.234276,0.253111,0.258613,0.292771,0.280753,0.261202,0.264294,0.269513,0.267916,0.26377,0.264697,0.277292,0.271994,0.266531,0.266999,0.270952,0.270634,0.271887,0.270707,0.271987,0.272505,0.274824,0.269903,0.273473,0.27108,0.265595,0.272789,0.272635,0.277917,0.270759,0.269593,0.266003,0.269985,0.274932,0.266606,0.271622], 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"AGGGT":864285, "AGGGC":1013001, "AGGGG":1014687, "TAAAA":1151239, "TAAAT":691210, "TAAAC":442714, "TAAAG":683774, "TAATA":500111, "TAATT":724056, "TAATC":497757, "TAATG":502395, "TAACA":508850, "TAACT":431511, "TAACC":341112, "TAACG":89609, "TAAGA":548091, "TAAGT":516999, "TAAGC":369458, "TAAGG":524778, "TATAA":507595, "TATAT":517009, "TATAC":306304, "TATAG":389775, "TATTA":510434, "TATTT":1129389, "TATTC":491579, "TATTG":499820, "TATCA":464257, "TATCT":500040, "TATCC":383380, "TATCG":89671, "TATGA":532006, "TATGT":558970, "TATGC":527637, "TATGG":549906, "TACAA":638590, "TACAT":508496, "TACAC":392091, "TACAG":818818, "TACTA":439884, "TACTT":568391, "TACTC":833361, "TACTG":560348, "TACCA":533886, "TACCT":555316, "TACCC":396252, "TACCG":148117, "TACGA":144580, "TACGT":126132, "TACGC":108587, "TACGG":128417, "TAGAA":666854, "TAGAT":407575, "TAGAC":310505, "TAGAG":718891, "TAGTA":385615, "TAGTT":435631, "TAGTC":605679, "TAGTG":704854, "TAGCA":472807, "TAGCT":596639, "TAGCC":447949, 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"C":[31.7513,31.7499,31.7683,31.8232,31.8332,35.4978,35.4751,35.4374,35.4311,35.4419,35.4519,35.4329,35.4268,35.4244,35.4202,35.4105,35.3985,35.3994,35.3853,35.3678,35.3552,35.3362,35.3013,35.2875,35.257,35.2115,35.183,35.1774,35.162,35.1408,35.128,35.1014,35.0847,35.0624,35.0415,35.0322,35.0078,34.9859,34.9715,34.9471,34.9424,34.9102,34.5292], "G":[31.2788,31.5891,31.6515,31.7228,31.7408,35.4962,35.4599,35.4512,35.4369,35.4616,35.4787,35.459,35.4501,35.4522,35.4389,35.4396,35.4325,35.4277,35.4217,35.4081,35.3969,35.3767,35.3418,35.3325,35.3132,35.2888,35.2559,35.2607,35.2416,35.2249,35.2271,35.1982,35.1753,35.1675,35.1582,35.1439,35.128,35.1108,35.088,35.0881,35.0533,35.0405,34.8218], "mean":[31.5336,31.6545,31.7027,31.7374,31.7521,35.4284,35.4444,35.44,35.4337,35.3872,35.4479,35.4463,35.4344,35.4342,35.4285,35.4262,35.4175,35.4131,35.4026,35.3889,35.3814,35.3651,35.338,35.3248,35.3041,35.2729,35.2462,35.2438,35.229,35.2054,35.2037,35.1835,35.165,35.1543,35.1441,35.1336,35.115,35.099,35.0804,35.0684,35.0536,35.0379,34.7357] }, "content_curves": { "A":[0.0928819,0.132014,0.184235,0.25188,0.285847,0.296403,0.22234,0.201122,0.204671,0.329513,0.246691,0.22319,0.246113,0.249556,0.24713,0.242236,0.246771,0.243611,0.245226,0.245544,0.244743,0.244934,0.247935,0.246683,0.248753,0.247934,0.245776,0.251984,0.243719,0.246016,0.243228,0.244166,0.245773,0.243887,0.248643,0.247996,0.246005,0.245902,0.247639,0.249376,0.25017,0.24964,0.248001], 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"GTTCC":683441, "GTTCG":198797, "GTTGA":549929, "GTTGT":595569, "GTTGC":681900, "GTTGG":701975, "GTCAA":570495, "GTCAT":603821, "GTCAC":818461, "GTCAG":840315, "GTCTA":344477, "GTCTT":982209, "GTCTC":1111887, "GTCTG":904963, "GTCCA":816613, "GTCCT":862867, "GTCCC":972962, "GTCCG":243418, "GTCGA":191508, "GTCGT":238788, "GTCGC":261509, "GTCGG":298917, "GTGAA":761236, "GTGAT":709168, "GTGAC":546204, "GTGAG":873048, "GTGTA":436116, "GTGTT":622431, "GTGTC":612214, "GTGTG":889116, "GTGCA":1036883, "GTGCT":760665, "GTGCC":809562, "GTGCG":277231, "GTGGA":785535, "GTGGT":1113186, "GTGGC":1385483, "GTGGG":1041552, "GCAAA":813077, "GCAAT":549324, "GCAAC":768335, "GCAAG":639618, "GCATA":397283, "GCATT":615723, "GCATC":589406, "GCATG":691325, "GCACA":842154, "GCACT":741426, "GCACC":739169, "GCACG":532893, "GCAGA":1080074, "GCAGT":1154391, "GCAGC":1181272, "GCAGG":1380629, "GCTAA":492350, "GCTAT":898633, "GCTAC":510375, "GCTAG":379218, "GCTTA":381408, "GCTTT":868901, "GCTTC":939312, "GCTTG":923536, 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"CTAGA":521841, "CTAGT":336825, "CTAGC":381794, "CTAGG":567916, "CTTAA":531223, "CTTAT":456250, "CTTAC":410997, "CTTAG":455371, "CTTTA":627056, "CTTTT":1194334, "CTTTC":903889, "CTTTG":1126600, "CTTCA":1026131, "CTTCT":1187579, "CTTCC":1363069, "CTTCG":377157, "CTTGA":1189703, "CTTGT":709666, "CTTGC":704832, "CTTGG":1067655, "CTCAA":847918, "CTCAT":795462, "CTCAC":994829, "CTCAG":1437032, "CTCTA":601981, "CTCTT":996964, "CTCTC":1158241, "CTCTG":1531842, "CTCCA":1240986, "CTCCT":1685259, "CTCCC":1942437, "CTCCG":681230, "CTCGA":278280, "CTCGT":309308, "CTCGC":557407, "CTCGG":1093072, "CTGAA":1149468, "CTGAT":835789, "CTGAC":833405, "CTGAG":1982790, "CTGTA":1287792, "CTGTT":923803, "CTGTC":1065883, "CTGTG":1398157, "CTGCA":1233150, "CTGCT":1251072, "CTGCC":1523666, "CTGCG":555674, "CTGGA":1704556, "CTGGT":1011517, "CTGGC":1475404, "CTGGG":2199817, "CCAAA":988397, "CCAAT":421996, "CCAAC":651032, "CCAAG":1132983, "CCATA":384639, "CCATT":793811, "CCATC":933119, "CCATG":1025405, "CCACA":967589, "CCACT":860481, "CCACC":1480558, "CCACG":461206, "CCAGA":1207888, "CCAGT":860230, "CCAGC":1947228, "CCAGG":2263182, "CCTAA":556804, "CCTAT":395606, "CCTAC":503323, "CCTAG":533346, "CCTTA":463844, "CCTTT":925950, "CCTTC":1153352, "CCTTG":935935, "CCTCA":1323431, "CCTCT":1251249, "CCTCC":2271196, "CCTCG":751955, "CCTGA":1316564, "CCTGT":1459749, "CCTGC":1411796, "CCTGG":1982013, "CCCAA":933725, "CCCAT":824589, "CCCAC":1105338, "CCCAG":2511397, "CCCTA":469190, "CCCTT":932944, "CCCTC":1558795, "CCCTG":1419165, "CCCCA":1387199, "CCCCT":1243491, "CCCCC":1647117, "CCCCG":1160818, "CCCGA":526618, "CCCGT":475838, "CCCGC":1148864, "CCCGG":1129445, "CCGAA":328997, "CCGAT":331217, "CCGAC":305010, "CCGAG":713505, "CCGTA":116436, "CCGTT":209157, "CCGTC":479761, "CCGTG":516127, "CCGCA":463634, "CCGCT":477295, "CCGCC":1548770, "CCGCG":945348, "CCGGA":441349, "CCGGT":406719, "CCGGC":1079433, "CCGGG":1064866, "CGAAA":225978, "CGAAT":138717, "CGAAC":250179, "CGAAG":345284, "CGATA":110551, "CGATT":146742, "CGATC":367165, "CGATG":200692, "CGACA":244652, "CGACT":194964, "CGACC":342343, "CGACG":270921, "CGAGA":503385, "CGAGT":328107, "CGAGC":394763, "CGAGG":711288, "CGTAA":99179, "CGTAT":96024, "CGTAC":105879, "CGTAG":125165, "CGTTA":102336, "CGTTT":242747, "CGTTC":286508, "CGTTG":218547, "CGTCA":219462, "CGTCT":323305, "CGTCC":597363, "CGTCG":295252, "CGTGA":389302, "CGTGT":491755, "CGTGC":697006, "CGTGG":703093, "CGCAA":198486, "CGCAT":162051, "CGCAC":387916, "CGCAG":417052, "CGCTA":342487, "CGCTT":570980, "CGCTC":600212, "CGCTG":430101, "CGCCA":524023, "CGCCT":722382, "CGCCC":1070616, "CGCCG":1028284, "CGCGA":251648, "CGCGT":557734, "CGCGC":846331, "CGCGG":797963, "CGGAA":370355, "CGGAT":192066, "CGGAC":303795, "CGGAG":633009, "CGGTA":87996, "CGGTT":226747, "CGGTC":438528, "CGGTG":693920, "CGGCA":404485, "CGGCT":663055, "CGGCC":1045291, "CGGCG":854902, "CGGGA":1030045, "CGGGT":646159, "CGGGC":864944, "CGGGG":1157643, "GAAAA":1395599, "GAAAT":942856, "GAAAC":855980, "GAAAG":1181592, "GAATA":503248, "GAATT":719845, "GAATC":548248, "GAATG":789320, "GAACA":797674, "GAACT":784308, "GAACC":749297, "GAACG":334731, "GAAGA":1576824, "GAAGT":870135, "GAAGC":1092088, "GAAGG":1445486, "GATAA":456030, "GATAT":398650, "GATAC":311568, "GATAG":368792, "GATTA":560752, "GATTT":746132, "GATTC":637946, "GATTG":547980, "GATCA":694470, "GATCT":808890, "GATCC":642540, "GATCG":760183, "GATGA":941732, "GATGT":658995, "GATGC":608030, "GATGG":967938, "GACAA":652978, "GACAT":648443, "GACAC":565879, "GACAG":965050, "GACTA":379315, "GACTT":675510, "GACTC":600410, "GACTG":744768, "GACCA":899306, "GACCT":922955, "GACCC":769623, "GACCG":400152, "GACGA":373796, "GACGT":266364, "GACGC":317585, "GACGG":439554, "GAGAA":1513364, "GAGAT":923859, "GAGAC":1107888, "GAGAG":1518003, "GAGTA":501937, "GAGTT":982467, "GAGTC":754804, "GAGTG":1005594, "GAGCA":959288, "GAGCT":1021056, "GAGCC":1366683, "GAGCG":587126, "GAGGA":1946876, "GAGGT":1280676, "GAGGC":2125616, "GAGGG":1252436, "GTAAA":534199, "GTAAT":512237, "GTAAC":305632, "GTAAG":371887, "GTATA":323214, "GTATT":551640, "GTATC":298572, "GTATG":395427, "GTACA":429115, "GTACT":373213, "GTACC":369975, "GTACG":108668, "GTAGA":561773, "GTAGT":901359, "GTAGC":480847, "GTAGG":391085, "GTTAA":431412, "GTTAT":390634, "GTTAC":369851, "GTTAG":353490, "GTTTA":531065, "GTTTT":1119928, "GTTTC":837214, "GTTTG":894912, "GTTCA":695697, "GTTCT":1006486, "GTTCC":742435, "GTTCG":414200, "GTTGA":682345, "GTTGT":590019, "GTTGC":695039, "GTTGG":825589, "GTCAA":516391, "GTCAT":554693, "GTCAC":678580, "GTCAG":749315, "GTCTA":302878, "GTCTT":767265, "GTCTC":959746, "GTCTG":813340, "GTCCA":702016, "GTCCT":799816, "GTCCC":1362472, "GTCCG":499827, "GTCGA":170738, "GTCGT":349976, "GTCGC":331723, "GTCGG":500714, "GTGAA":1003172, "GTGAT":811338, "GTGAC":784765, "GTGAG":1082159, "GTGTA":465372, "GTGTT":789356, "GTGTC":967746, "GTGTG":1307665, "GTGCA":807383, "GTGCT":886867, "GTGCC":1227634, "GTGCG":756419, "GTGGA":1125750, "GTGGT":1093734, "GTGGC":1426999, "GTGGG":1603067, "GCAAA":745334, "GCAAT":482133, "GCAAC":538370, "GCAAG":779269, "GCATA":311524, "GCATT":576116, "GCATC":627600, "GCATG":711728, "GCACA":729859, "GCACT":784249, "GCACC":718506, "GCACG":321447, "GCAGA":1153096, "GCAGT":988420, "GCAGC":1129103, "GCAGG":1296559, "GCTAA":465785, "GCTAT":573789, "GCTAC":862691, "GCTAG":433673, "GCTTA":368999, "GCTTT":787917, "GCTTC":862950, "GCTTG":971811, "GCTCA":973532, "GCTCT":942280, "GCTCC":1065980, "GCTCG":412468, "GCTGA":1676450, "GCTGT":1216419, "GCTGC":1267918, "GCTGG":2007432, "GCCAA":839607, "GCCAT":796120, "GCCAC":1008315, "GCCAG":1362266, "GCCTA":513354, "GCCTT":879544, "GCCTC":1563344, "GCCTG":1841154, "GCCCA":1342122, "GCCCT":1135210, "GCCCC":1414860, "GCCCG":991520, "GCCGA":628541, "GCCGT":368338, "GCCGC":1218853, "GCCGG":902054, "GCGAA":208434, "GCGAT":221393, "GCGAC":323375, "GCGAG":450588, "GCGTA":85396, "GCGTT":214622, "GCGTC":446528, "GCGTG":900045, "GCGCA":317560, "GCGCT":618028, "GCGCC":847537, "GCGCG":989009, "GCGGA":422661, "GCGGT":501811, "GCGGC":993941, "GCGGG":1073117, "GGAAA":1303776, "GGAAT":717698, "GGAAC":688468, "GGAAG":1628725, "GGATA":397151, "GGATT":778193, "GGATC":863730, "GGATG":897107, "GGACA":843144, "GGACT":730881, "GGACC":895152, "GGACG":440273, "GGAGA":1598173, "GGAGT":1270930, "GGAGC":1341626, "GGAGG":2843052, "GGTAA":377388, "GGTAT":364888, "GGTAC":311008, "GGTAG":432251, "GGTTA":336961, "GGTTT":824709, "GGTTC":699376, "GGTTG":885692, "GGTCA":721855, "GGTCT":813217, "GGTCC":671774, "GGTCG":468499, "GGTGA":1119752, "GGTGT":1079732, "GGTGC":956725, "GGTGG":2021517, "GGCAA":745400, "GGCAT":808420, "GGCAC":765492, "GGCAG":1388000, "GGCTA":554020, "GGCTT":807298, "GGCTC":1127885, "GGCTG":2730665, "GGCCA":1208436, "GGCCT":1133841, "GGCCC":1534270, "GGCCG":1000161, "GGCGA":397082, "GGCGT":530262, "GGCGC":895328, "GGCGG":1258494, "GGGAA":1177875, "GGGAT":801681, "GGGAC":917099, "GGGAG":2406694, "GGGTA":380964, "GGGTT":946706, "GGGTC":766937, "GGGTG":1372008, "GGGCA":991580, "GGGCT":1289062, "GGGCC":1413206, "GGGCG":865945, "GGGGA":1377396, "GGGGT":1031463, "GGGGC":1319822, "GGGGG":1384224 }, "overrepresented_sequences": { } }, "command": "fastp -w 2 --in1 /scratch/siebrenf/hendrik/fastq/White-Bloodcells-Morsche-MDL1-C-nonstimulated-0-16238_R1.fastq.gz --in2 /scratch/siebrenf/hendrik/fastq/White-Bloodcells-Morsche-MDL1-C-nonstimulated-0-16238_R2.fastq.gz --out1 /scratch/siebrenf/hendrik/results2/fastq_trimmed/White-Bloodcells-Morsche-MDL1-C-nonstimulated-0-16238_R1_trimmed.fastq.gz --out2 /scratch/siebrenf/hendrik/results2/fastq_trimmed/White-Bloodcells-Morsche-MDL1-C-nonstimulated-0-16238_R2_trimmed.fastq.gz -h /scratch/siebrenf/hendrik/results2/qc/trimming/White-Bloodcells-Morsche-MDL1-C-nonstimulated-0-16238.fastp.html -j /scratch/siebrenf/hendrik/results2/qc/trimming/White-Bloodcells-Morsche-MDL1-C-nonstimulated-0-16238.fastp.json " }