## htsjdk.samtools.metrics.StringHeader # MarkDuplicates MAX_FILE_HANDLES_FOR_READ_ENDS_MAP=999 INPUT=[/ceph/rimlsfnwi/data/moldevbio/heeringen/siebrenf/ghe_2021/results/star/hg38-NP_Day1_adherentcells_TA_A66_S11.samtools-coordinate-sieved.bam] OUTPUT=/ceph/rimlsfnwi/data/moldevbio/heeringen/siebrenf/ghe_2021/results/final_bam/hg38-NP_Day1_adherentcells_TA_A66_S11.samtools-coordinate.bam METRICS_FILE=/ceph/rimlsfnwi/data/moldevbio/heeringen/siebrenf/ghe_2021/results/qc/markdup/hg38-NP_Day1_adherentcells_TA_A66_S11.samtools-coordinate.metrics.txt MAX_SEQUENCES_FOR_DISK_READ_ENDS_MAP=50000 SORTING_COLLECTION_SIZE_RATIO=0.25 TAG_DUPLICATE_SET_MEMBERS=false REMOVE_SEQUENCING_DUPLICATES=false TAGGING_POLICY=DontTag CLEAR_DT=true DUPLEX_UMI=false ADD_PG_TAG_TO_READS=true REMOVE_DUPLICATES=false ASSUME_SORTED=false DUPLICATE_SCORING_STRATEGY=SUM_OF_BASE_QUALITIES PROGRAM_RECORD_ID=MarkDuplicates PROGRAM_GROUP_NAME=MarkDuplicates READ_NAME_REGEX= OPTICAL_DUPLICATE_PIXEL_DISTANCE=100 MAX_OPTICAL_DUPLICATE_SET_SIZE=300000 VERBOSITY=INFO QUIET=false VALIDATION_STRINGENCY=STRICT COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false GA4GH_CLIENT_SECRETS=client_secrets.json USE_JDK_DEFLATER=false USE_JDK_INFLATER=false ## htsjdk.samtools.metrics.StringHeader # Started on: Mon Aug 30 14:02:52 CEST 2021 ## METRICS CLASS picard.sam.DuplicationMetrics LIBRARY UNPAIRED_READS_EXAMINED READ_PAIRS_EXAMINED SECONDARY_OR_SUPPLEMENTARY_RDS UNMAPPED_READS UNPAIRED_READ_DUPLICATES READ_PAIR_DUPLICATES READ_PAIR_OPTICAL_DUPLICATES PERCENT_DUPLICATION ESTIMATED_LIBRARY_SIZE Unknown Library 232 19755632 0 0 110 2324108 5765 0.117645 77403304 ## HISTOGRAM java.lang.Double BIN CoverageMult all_sets optical_sets non_optical_sets 1.0 1.000256 15700319 0 15704845 2.0 1.775193 1490644 5763 1487080 3.0 2.375567 163364 1 162544 4.0 2.840699 34256 0 34154 5.0 3.201055 13327 0 13299 6.0 3.480236 6822 0 6816 7.0 3.696529 4319 0 4318 8.0 3.864099 2810 0 2810 9.0 3.993922 2035 0 2035 10.0 4.094501 1485 0 1482 11.0 4.172424 1081 0 1082 12.0 4.232793 870 0 869 13.0 4.279564 716 0 715 14.0 4.315799 596 0 596 15.0 4.343871 471 0 472 16.0 4.36562 442 0 441 17.0 4.38247 348 0 347 18.0 4.395524 315 0 316 19.0 4.405638 252 0 251 20.0 4.413473 236 0 237 21.0 4.419543 195 0 195 22.0 4.424246 196 0 196 23.0 4.42789 185 0 186 24.0 4.430713 149 0 147 25.0 4.432899 129 0 131 26.0 4.434594 135 0 135 27.0 4.435906 112 0 111 28.0 4.436923 118 0 118 29.0 4.437711 88 0 89 30.0 4.438322 108 0 106 31.0 4.438795 82 0 83 32.0 4.439161 57 0 57 33.0 4.439445 68 0 68 34.0 4.439665 62 0 62 35.0 4.439835 59 0 58 36.0 4.439967 59 0 59 37.0 4.440069 52 0 52 38.0 4.440148 44 0 44 39.0 4.44021 45 0 45 40.0 4.440257 44 0 45 41.0 4.440294 41 0 40 42.0 4.440323 35 0 35 43.0 4.440345 41 0 43 44.0 4.440362 39 0 37 45.0 4.440375 28 0 28 46.0 4.440386 33 0 33 47.0 4.440394 32 0 33 48.0 4.4404 29 0 28 49.0 4.440404 38 0 38 50.0 4.440408 25 0 25 51.0 4.440411 19 0 19 52.0 4.440413 20 0 20 53.0 4.440415 31 0 31 54.0 4.440416 18 0 18 55.0 4.440417 22 0 22 56.0 4.440418 19 0 19 57.0 4.440419 20 0 20 58.0 4.440419 24 0 24 59.0 4.44042 13 0 13 60.0 4.44042 14 0 14 61.0 4.44042 12 0 12 62.0 4.44042 20 0 20 63.0 4.44042 12 0 12 64.0 4.440421 11 0 11 65.0 4.440421 19 0 19 66.0 4.440421 12 0 12 67.0 4.440421 10 0 10 68.0 4.440421 7 0 7 69.0 4.440421 11 0 11 70.0 4.440421 13 0 13 71.0 4.440421 16 0 16 72.0 4.440421 14 0 14 73.0 4.440421 5 0 5 74.0 4.440421 8 0 8 75.0 4.440421 7 0 7 76.0 4.440421 8 0 8 77.0 4.440421 10 0 10 78.0 4.440421 8 0 9 79.0 4.440421 14 0 13 80.0 4.440421 10 0 10 81.0 4.440421 9 0 9 82.0 4.440421 6 0 6 83.0 4.440421 5 0 5 84.0 4.440421 5 0 5 85.0 4.440421 5 0 5 86.0 4.440421 4 0 4 87.0 4.440421 7 0 7 88.0 4.440421 4 0 4 89.0 4.440421 5 0 5 90.0 4.440421 6 0 6 91.0 4.440421 6 0 6 92.0 4.440421 4 0 5 93.0 4.440421 7 0 6 94.0 4.440421 6 0 6 95.0 4.440421 4 0 4 96.0 4.440421 5 0 5 97.0 4.440421 6 0 6 98.0 4.440421 7 0 7 99.0 4.440421 4 0 4 100.0 4.440421 1 0 1 101.0 0 5 0 6 102.0 0 7 0 6 103.0 0 9 0 9 104.0 0 8 0 9 105.0 0 5 0 4 106.0 0 2 0 2 107.0 0 5 0 5 108.0 0 2 0 2 109.0 0 5 0 5 110.0 0 10 0 10 111.0 0 4 0 4 112.0 0 3 0 3 113.0 0 4 0 4 114.0 0 3 0 3 115.0 0 3 0 3 116.0 0 5 0 5 117.0 0 2 0 2 118.0 0 1 0 1 120.0 0 3 0 3 121.0 0 2 0 4 122.0 0 4 0 2 123.0 0 4 0 5 124.0 0 4 0 3 125.0 0 5 0 5 126.0 0 3 0 3 127.0 0 2 0 2 128.0 0 2 0 2 129.0 0 2 0 2 130.0 0 2 0 2 131.0 0 2 0 2 132.0 0 4 0 4 133.0 0 1 0 1 134.0 0 5 0 5 135.0 0 3 0 3 136.0 0 1 0 2 137.0 0 3 0 2 138.0 0 4 0 5 139.0 0 3 0 2 141.0 0 2 0 2 142.0 0 4 0 4 143.0 0 4 0 4 144.0 0 2 0 2 145.0 0 5 0 5 146.0 0 3 0 3 147.0 0 1 0 1 148.0 0 2 0 2 149.0 0 4 0 4 150.0 0 1 0 1 151.0 0 1 0 1 152.0 0 3 0 3 154.0 0 1 0 1 155.0 0 1 0 1 157.0 0 1 0 1 159.0 0 2 0 2 160.0 0 1 0 1 161.0 0 1 0 1 162.0 0 1 0 1 163.0 0 1 0 1 164.0 0 1 0 1 165.0 0 1 0 1 166.0 0 1 0 1 168.0 0 1 0 1 169.0 0 1 0 1 170.0 0 3 0 3 171.0 0 1 0 1 172.0 0 1 0 1 173.0 0 1 0 1 174.0 0 1 0 1 175.0 0 1 0 1 176.0 0 3 0 3 177.0 0 2 0 2 179.0 0 1 0 1 180.0 0 1 0 1 181.0 0 3 0 3 184.0 0 2 0 2 185.0 0 0 0 1 186.0 0 4 0 3 188.0 0 2 0 2 189.0 0 1 0 1 191.0 0 1 0 1 193.0 0 1 0 1 194.0 0 1 0 1 195.0 0 2 0 2 196.0 0 1 0 1 197.0 0 1 0 1 199.0 0 1 0 1 200.0 0 2 0 2 201.0 0 1 0 1 202.0 0 1 0 1 203.0 0 1 0 1 204.0 0 1 0 1 206.0 0 3 0 3 208.0 0 1 0 2 209.0 0 1 0 0 210.0 0 1 0 1 211.0 0 1 0 1 212.0 0 1 0 1 215.0 0 1 0 1 216.0 0 1 0 1 220.0 0 1 0 1 222.0 0 2 0 2 224.0 0 2 0 2 225.0 0 3 0 3 228.0 0 1 0 1 230.0 0 1 0 1 233.0 0 2 0 2 235.0 0 1 0 1 238.0 0 1 0 1 239.0 0 1 0 1 241.0 0 1 0 1 242.0 0 1 0 1 244.0 0 1 0 1 245.0 0 2 0 3 246.0 0 1 0 0 248.0 0 0 0 1 249.0 0 2 0 2 250.0 0 2 0 1 251.0 0 0 0 1 252.0 0 2 0 1 254.0 0 2 0 2 255.0 0 2 0 2 256.0 0 1 0 1 258.0 0 1 0 2 259.0 0 2 0 1 263.0 0 1 0 1 264.0 0 1 0 1 265.0 0 1 0 1 267.0 0 1 0 1 270.0 0 2 0 2 271.0 0 1 0 1 272.0 0 1 0 1 278.0 0 1 0 1 279.0 0 1 0 1 281.0 0 1 0 1 284.0 0 1 0 1 285.0 0 1 0 1 286.0 0 1 0 1 295.0 0 1 0 1 297.0 0 1 0 1 300.0 0 1 0 1 310.0 0 1 0 1 317.0 0 1 0 1 319.0 0 1 0 1 326.0 0 0 0 1 327.0 0 2 0 1 338.0 0 1 0 1 371.0 0 0 0 1 372.0 0 1 0 0 378.0 0 1 0 1 381.0 0 0 0 1 382.0 0 1 0 0 386.0 0 1 0 1 389.0 0 1 0 1 393.0 0 1 0 1 399.0 0 2 0 2 417.0 0 1 0 1 419.0 0 0 0 1 420.0 0 1 0 0 438.0 0 1 0 1 448.0 0 1 0 1 452.0 0 1 0 1 454.0 0 0 0 1 455.0 0 1 0 0 463.0 0 1 0 1 468.0 0 0 0 1 469.0 0 1 0 0 476.0 0 1 0 1 502.0 0 1 0 1 504.0 0 1 0 1 519.0 0 1 0 1 533.0 0 0 0 1 534.0 0 1 0 0 547.0 0 1 0 1 550.0 0 0 0 1 551.0 0 1 0 0 658.0 0 1 0 1 663.0 0 1 0 1 667.0 0 0 0 1 668.0 0 1 0 1 669.0 0 1 0 0 688.0 0 1 0 1 711.0 0 0 0 1 712.0 0 1 0 0 798.0 0 1 0 1 799.0 0 1 0 1 804.0 0 1 0 1 816.0 0 0 0 1 817.0 0 1 0 0 907.0 0 1 0 1 931.0 0 1 0 1