## htsjdk.samtools.metrics.StringHeader # MarkDuplicates MAX_FILE_HANDLES_FOR_READ_ENDS_MAP=999 INPUT=[/ceph/rimlsfnwi/data/moldevbio/heeringen/siebrenf/ghe_2021/results/star/hg38-NP_Day1_adherentcells_DMSO_A70_S14.samtools-coordinate-sieved.bam] OUTPUT=/ceph/rimlsfnwi/data/moldevbio/heeringen/siebrenf/ghe_2021/results/final_bam/hg38-NP_Day1_adherentcells_DMSO_A70_S14.samtools-coordinate.bam METRICS_FILE=/ceph/rimlsfnwi/data/moldevbio/heeringen/siebrenf/ghe_2021/results/qc/markdup/hg38-NP_Day1_adherentcells_DMSO_A70_S14.samtools-coordinate.metrics.txt MAX_SEQUENCES_FOR_DISK_READ_ENDS_MAP=50000 SORTING_COLLECTION_SIZE_RATIO=0.25 TAG_DUPLICATE_SET_MEMBERS=false REMOVE_SEQUENCING_DUPLICATES=false TAGGING_POLICY=DontTag CLEAR_DT=true DUPLEX_UMI=false ADD_PG_TAG_TO_READS=true REMOVE_DUPLICATES=false ASSUME_SORTED=false DUPLICATE_SCORING_STRATEGY=SUM_OF_BASE_QUALITIES PROGRAM_RECORD_ID=MarkDuplicates PROGRAM_GROUP_NAME=MarkDuplicates READ_NAME_REGEX= OPTICAL_DUPLICATE_PIXEL_DISTANCE=100 MAX_OPTICAL_DUPLICATE_SET_SIZE=300000 VERBOSITY=INFO QUIET=false VALIDATION_STRINGENCY=STRICT COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false GA4GH_CLIENT_SECRETS=client_secrets.json USE_JDK_DEFLATER=false USE_JDK_INFLATER=false ## htsjdk.samtools.metrics.StringHeader # Started on: Mon Aug 30 14:05:10 CEST 2021 ## METRICS CLASS picard.sam.DuplicationMetrics LIBRARY UNPAIRED_READS_EXAMINED READ_PAIRS_EXAMINED SECONDARY_OR_SUPPLEMENTARY_RDS UNMAPPED_READS UNPAIRED_READ_DUPLICATES READ_PAIR_DUPLICATES READ_PAIR_OPTICAL_DUPLICATES PERCENT_DUPLICATION ESTIMATED_LIBRARY_SIZE Unknown Library 225 17260223 0 0 107 1565702 5131 0.090714 89551145 ## HISTOGRAM java.lang.Double BIN CoverageMult all_sets optical_sets non_optical_sets 1.0 1.00027 14612531 0 14616826 2.0 1.825187 924054 5125 920416 3.0 2.505493 94854 3 94288 4.0 3.066537 24593 0 24520 5.0 3.529228 11173 0 11165 6.0 3.910807 6206 0 6204 7.0 4.225493 3790 0 3786 8.0 4.485013 2517 0 2514 9.0 4.699038 1870 0 1875 10.0 4.875543 1358 0 1354 11.0 5.021106 1021 0 1022 12.0 5.141152 920 0 919 13.0 5.240152 700 0 698 14.0 5.321798 611 0 612 15.0 5.38913 525 0 526 16.0 5.444659 436 0 434 17.0 5.490453 368 0 368 18.0 5.52822 300 0 302 19.0 5.559365 248 0 247 20.0 5.585051 263 0 266 21.0 5.606234 214 0 211 22.0 5.623704 207 0 206 23.0 5.63811 182 0 183 24.0 5.649992 152 0 152 25.0 5.65979 131 0 130 26.0 5.667871 121 0 122 27.0 5.674535 114 0 113 28.0 5.680031 114 0 114 29.0 5.684564 95 0 95 30.0 5.688302 102 0 102 31.0 5.691384 97 0 97 32.0 5.693926 74 0 74 33.0 5.696023 73 0 74 34.0 5.697752 65 0 64 35.0 5.699178 58 0 58 36.0 5.700354 54 0 54 37.0 5.701324 48 0 48 38.0 5.702123 51 0 51 39.0 5.702783 37 0 38 40.0 5.703327 45 0 44 41.0 5.703775 42 0 42 42.0 5.704145 45 0 45 43.0 5.704451 43 0 44 44.0 5.704702 40 0 39 45.0 5.70491 33 0 33 46.0 5.705081 34 0 34 47.0 5.705222 31 0 31 48.0 5.705338 30 0 30 49.0 5.705434 26 0 26 50.0 5.705513 18 0 18 51.0 5.705579 26 0 27 52.0 5.705633 28 0 27 53.0 5.705677 29 0 29 54.0 5.705714 26 0 26 55.0 5.705744 11 0 11 56.0 5.705769 26 0 26 57.0 5.705789 15 0 15 58.0 5.705806 14 0 14 59.0 5.70582 20 0 20 60.0 5.705832 12 0 13 61.0 5.705841 19 0 18 62.0 5.705849 15 0 15 63.0 5.705855 16 0 16 64.0 5.705861 5 0 5 65.0 5.705865 17 0 17 66.0 5.705869 11 0 11 67.0 5.705872 9 0 9 68.0 5.705874 9 0 9 69.0 5.705876 13 0 13 70.0 5.705878 13 0 13 71.0 5.705879 11 0 11 72.0 5.70588 10 0 10 73.0 5.705881 6 0 6 74.0 5.705882 12 0 13 75.0 5.705883 11 0 10 76.0 5.705883 6 0 7 77.0 5.705884 13 0 12 78.0 5.705884 11 0 11 79.0 5.705884 9 0 9 80.0 5.705885 4 0 4 81.0 5.705885 11 0 11 82.0 5.705885 8 0 8 83.0 5.705885 14 0 14 84.0 5.705885 7 0 7 85.0 5.705885 6 0 6 86.0 5.705885 4 0 5 87.0 5.705886 9 0 8 88.0 5.705886 3 0 3 89.0 5.705886 5 0 5 90.0 5.705886 2 0 2 91.0 5.705886 4 0 4 92.0 5.705886 6 0 6 93.0 5.705886 4 0 4 94.0 5.705886 13 0 13 95.0 5.705886 5 0 6 96.0 5.705886 8 0 7 97.0 5.705886 6 0 6 98.0 5.705886 3 0 3 99.0 5.705886 12 0 12 100.0 5.705886 3 0 3 101.0 0 7 0 7 102.0 0 6 0 6 103.0 0 6 0 7 104.0 0 4 0 3 105.0 0 5 0 5 106.0 0 7 0 7 107.0 0 3 0 3 108.0 0 4 0 4 109.0 0 3 0 3 110.0 0 4 0 4 111.0 0 3 0 3 112.0 0 6 0 6 113.0 0 3 0 3 114.0 0 5 0 6 115.0 0 4 0 4 116.0 0 6 0 5 117.0 0 2 0 2 118.0 0 3 0 3 119.0 0 4 0 4 120.0 0 1 0 1 121.0 0 3 0 3 122.0 0 2 0 2 123.0 0 4 0 4 124.0 0 7 0 7 125.0 0 6 0 6 127.0 0 4 0 4 128.0 0 3 0 3 129.0 0 3 0 3 130.0 0 3 0 3 131.0 0 1 0 1 132.0 0 3 0 3 133.0 0 1 0 1 134.0 0 1 0 1 136.0 0 2 0 2 137.0 0 4 0 4 138.0 0 2 0 2 139.0 0 1 0 1 141.0 0 3 0 3 143.0 0 2 0 2 145.0 0 2 0 2 146.0 0 2 0 2 147.0 0 1 0 1 148.0 0 2 0 2 150.0 0 1 0 1 152.0 0 1 0 1 153.0 0 5 0 5 154.0 0 3 0 3 156.0 0 1 0 1 157.0 0 2 0 2 158.0 0 2 0 2 159.0 0 1 0 1 161.0 0 1 0 1 162.0 0 1 0 1 163.0 0 1 0 1 164.0 0 1 0 1 165.0 0 1 0 1 166.0 0 3 0 3 167.0 0 1 0 1 171.0 0 1 0 1 172.0 0 3 0 3 173.0 0 2 0 2 174.0 0 2 0 2 175.0 0 2 0 2 176.0 0 5 0 5 177.0 0 3 0 3 179.0 0 1 0 1 180.0 0 1 0 1 182.0 0 1 0 1 183.0 0 1 0 1 184.0 0 2 0 2 186.0 0 1 0 1 191.0 0 1 0 1 192.0 0 1 0 1 193.0 0 1 0 1 194.0 0 2 0 2 196.0 0 1 0 1 198.0 0 1 0 1 200.0 0 2 0 2 203.0 0 1 0 1 206.0 0 2 0 2 207.0 0 1 0 1 208.0 0 1 0 1 209.0 0 1 0 1 211.0 0 1 0 1 212.0 0 2 0 2 215.0 0 1 0 1 220.0 0 2 0 2 221.0 0 2 0 2 223.0 0 1 0 1 225.0 0 1 0 1 227.0 0 1 0 1 228.0 0 1 0 1 235.0 0 1 0 1 237.0 0 1 0 1 239.0 0 1 0 1 240.0 0 3 0 3 241.0 0 1 0 1 243.0 0 1 0 1 244.0 0 1 0 1 245.0 0 1 0 1 246.0 0 2 0 2 248.0 0 1 0 1 251.0 0 1 0 1 253.0 0 2 0 2 255.0 0 1 0 1 258.0 0 1 0 1 260.0 0 2 0 2 261.0 0 1 0 1 265.0 0 1 0 1 267.0 0 1 0 1 271.0 0 1 0 1 282.0 0 1 0 1 285.0 0 2 0 2 290.0 0 1 0 1 298.0 0 1 0 1 307.0 0 1 0 1 310.0 0 1 0 1 320.0 0 1 0 1 329.0 0 1 0 1 332.0 0 0 0 1 333.0 0 2 0 1 336.0 0 0 0 1 337.0 0 1 0 0 344.0 0 1 0 1 351.0 0 1 0 1 356.0 0 1 0 1 366.0 0 1 0 1 369.0 0 1 0 1 379.0 0 1 0 1 381.0 0 1 0 1 388.0 0 1 0 1 405.0 0 1 0 2 406.0 0 1 0 0 448.0 0 1 0 1 449.0 0 1 0 1 459.0 0 2 0 2 460.0 0 1 0 1 469.0 0 1 0 1 476.0 0 0 0 1 477.0 0 1 0 0 541.0 0 0 0 1 543.0 0 1 0 0 605.0 0 1 0 1 625.0 0 1 0 1 661.0 0 1 0 1 700.0 0 1 0 1 703.0 0 0 0 1 704.0 0 1 0 0 710.0 0 1 0 1 741.0 0 1 0 1 762.0 0 0 0 1 763.0 0 1 0 0 769.0 0 1 0 1 893.0 0 1 0 1 963.0 0 1 0 1