## htsjdk.samtools.metrics.StringHeader # MarkDuplicates MAX_FILE_HANDLES_FOR_READ_ENDS_MAP=999 INPUT=[/ceph/rimlsfnwi/data/moldevbio/heeringen/siebrenf/ghe_2021/results/star/hg38-NP_Day19_adherent_TA_A64_S1.samtools-coordinate-sieved.bam] OUTPUT=/ceph/rimlsfnwi/data/moldevbio/heeringen/siebrenf/ghe_2021/results/final_bam/hg38-NP_Day19_adherent_TA_A64_S1.samtools-coordinate.bam METRICS_FILE=/ceph/rimlsfnwi/data/moldevbio/heeringen/siebrenf/ghe_2021/results/qc/markdup/hg38-NP_Day19_adherent_TA_A64_S1.samtools-coordinate.metrics.txt MAX_SEQUENCES_FOR_DISK_READ_ENDS_MAP=50000 SORTING_COLLECTION_SIZE_RATIO=0.25 TAG_DUPLICATE_SET_MEMBERS=false REMOVE_SEQUENCING_DUPLICATES=false TAGGING_POLICY=DontTag CLEAR_DT=true DUPLEX_UMI=false ADD_PG_TAG_TO_READS=true REMOVE_DUPLICATES=false ASSUME_SORTED=false DUPLICATE_SCORING_STRATEGY=SUM_OF_BASE_QUALITIES PROGRAM_RECORD_ID=MarkDuplicates PROGRAM_GROUP_NAME=MarkDuplicates READ_NAME_REGEX= OPTICAL_DUPLICATE_PIXEL_DISTANCE=100 MAX_OPTICAL_DUPLICATE_SET_SIZE=300000 VERBOSITY=INFO QUIET=false VALIDATION_STRINGENCY=STRICT COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false GA4GH_CLIENT_SECRETS=client_secrets.json USE_JDK_DEFLATER=false USE_JDK_INFLATER=false ## htsjdk.samtools.metrics.StringHeader # Started on: Mon Aug 30 14:11:27 CEST 2021 ## METRICS CLASS picard.sam.DuplicationMetrics LIBRARY UNPAIRED_READS_EXAMINED READ_PAIRS_EXAMINED SECONDARY_OR_SUPPLEMENTARY_RDS UNMAPPED_READS UNPAIRED_READ_DUPLICATES READ_PAIR_DUPLICATES READ_PAIR_OPTICAL_DUPLICATES PERCENT_DUPLICATION ESTIMATED_LIBRARY_SIZE Unknown Library 106 16386698 0 0 50 6134534 3651 0.374361 15996313 ## HISTOGRAM java.lang.Double BIN CoverageMult all_sets optical_sets non_optical_sets 1.0 1.000128 6392174 0 6393376 2.0 1.359184 2531218 3641 2531226 3.0 1.488089 894980 5 894477 4.0 1.534367 284530 0 284132 5.0 1.550981 86448 0 86263 6.0 1.556946 27569 0 27480 7.0 1.559087 10717 0 10691 8.0 1.559856 5413 0 5411 9.0 1.560132 3294 0 3291 10.0 1.560231 2299 0 2295 11.0 1.560267 1781 0 1784 12.0 1.560279 1275 0 1274 13.0 1.560284 1049 0 1048 14.0 1.560286 824 0 823 15.0 1.560286 668 0 670 16.0 1.560286 521 0 522 17.0 1.560286 468 0 465 18.0 1.560286 408 0 409 19.0 1.560286 357 0 356 20.0 1.560286 356 0 359 21.0 1.560286 267 0 264 22.0 1.560286 236 0 237 23.0 1.560286 235 0 234 24.0 1.560286 202 0 202 25.0 1.560286 188 0 191 26.0 1.560286 160 0 157 27.0 1.560286 161 0 162 28.0 1.560286 147 0 146 29.0 1.560286 128 0 128 30.0 1.560286 113 0 114 31.0 1.560286 105 0 105 32.0 1.560286 87 0 86 33.0 1.560286 99 0 100 34.0 1.560286 87 0 86 35.0 1.560286 83 0 83 36.0 1.560286 76 0 76 37.0 1.560286 60 0 61 38.0 1.560286 72 0 72 39.0 1.560286 71 0 70 40.0 1.560286 66 0 66 41.0 1.560286 54 0 54 42.0 1.560286 60 0 60 43.0 1.560286 53 0 53 44.0 1.560286 45 0 45 45.0 1.560286 41 0 41 46.0 1.560286 50 0 50 47.0 1.560286 36 0 37 48.0 1.560286 40 0 39 49.0 1.560286 31 0 31 50.0 1.560286 28 0 29 51.0 1.560286 32 0 31 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