## htsjdk.samtools.metrics.StringHeader # MarkDuplicates MAX_FILE_HANDLES_FOR_READ_ENDS_MAP=999 INPUT=[/ceph/rimlsfnwi/data/moldevbio/heeringen/siebrenf/ghe_2021/results/star/hg38-NP_Day12_adherent_TA_A60_S4.samtools-coordinate-sieved.bam] OUTPUT=/ceph/rimlsfnwi/data/moldevbio/heeringen/siebrenf/ghe_2021/results/final_bam/hg38-NP_Day12_adherent_TA_A60_S4.samtools-coordinate.bam METRICS_FILE=/ceph/rimlsfnwi/data/moldevbio/heeringen/siebrenf/ghe_2021/results/qc/markdup/hg38-NP_Day12_adherent_TA_A60_S4.samtools-coordinate.metrics.txt MAX_SEQUENCES_FOR_DISK_READ_ENDS_MAP=50000 SORTING_COLLECTION_SIZE_RATIO=0.25 TAG_DUPLICATE_SET_MEMBERS=false REMOVE_SEQUENCING_DUPLICATES=false TAGGING_POLICY=DontTag CLEAR_DT=true DUPLEX_UMI=false ADD_PG_TAG_TO_READS=true REMOVE_DUPLICATES=false ASSUME_SORTED=false DUPLICATE_SCORING_STRATEGY=SUM_OF_BASE_QUALITIES PROGRAM_RECORD_ID=MarkDuplicates PROGRAM_GROUP_NAME=MarkDuplicates READ_NAME_REGEX= OPTICAL_DUPLICATE_PIXEL_DISTANCE=100 MAX_OPTICAL_DUPLICATE_SET_SIZE=300000 VERBOSITY=INFO QUIET=false VALIDATION_STRINGENCY=STRICT COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false GA4GH_CLIENT_SECRETS=client_secrets.json USE_JDK_DEFLATER=false USE_JDK_INFLATER=false ## htsjdk.samtools.metrics.StringHeader # Started on: Mon Aug 30 14:07:45 CEST 2021 ## METRICS CLASS picard.sam.DuplicationMetrics LIBRARY UNPAIRED_READS_EXAMINED READ_PAIRS_EXAMINED SECONDARY_OR_SUPPLEMENTARY_RDS UNMAPPED_READS UNPAIRED_READ_DUPLICATES READ_PAIR_DUPLICATES READ_PAIR_OPTICAL_DUPLICATES PERCENT_DUPLICATION ESTIMATED_LIBRARY_SIZE Unknown Library 224 18573011 0 0 144 2705068 6567 0.145648 57519557 ## HISTOGRAM java.lang.Double BIN CoverageMult all_sets optical_sets non_optical_sets 1.0 1.0003 13881608 0 13886225 2.0 1.724564 1640051 6553 1636839 3.0 2.248965 238523 7 237447 4.0 2.628656 52439 0 52197 5.0 2.90357 19230 0 19172 6.0 3.10262 10179 0 10167 7.0 3.246742 6085 0 6081 8.0 3.351094 3988 0 3979 9.0 3.426649 2795 0 2799 10.0 3.481354 2037 0 2035 11.0 3.520963 1583 0 1583 12.0 3.549642 1123 0 1119 13.0 3.570407 922 0 924 14.0 3.585442 756 0 754 15.0 3.596328 590 0 591 16.0 3.60421 510 0 510 17.0 3.609917 383 0 383 18.0 3.614049 321 0 319 19.0 3.617041 321 0 320 20.0 3.619207 241 0 243 21.0 3.620775 214 0 214 22.0 3.621911 199 0 199 23.0 3.622733 178 0 178 24.0 3.623329 155 0 153 25.0 3.62376 136 0 137 26.0 3.624072 106 0 106 27.0 3.624298 96 0 97 28.0 3.624461 106 0 104 29.0 3.62458 76 0 76 30.0 3.624666 60 0 61 31.0 3.624728 71 0 73 32.0 3.624773 66 0 64 33.0 3.624805 53 0 54 34.0 3.624829 56 0 54 35.0 3.624846 54 0 54 36.0 3.624858 40 0 41 37.0 3.624867 49 0 48 38.0 3.624874 42 0 42 39.0 3.624878 40 0 40 40.0 3.624882 20 0 20 41.0 3.624884 28 0 28 42.0 3.624886 23 0 23 43.0 3.624887 33 0 33 44.0 3.624888 29 0 29 45.0 3.624889 17 0 17 46.0 3.624889 28 0 28 47.0 3.62489 34 0 35 48.0 3.62489 18 0 17 49.0 3.62489 17 0 17 50.0 3.62489 18 0 18 51.0 3.62489 19 0 19 52.0 3.62489 12 0 14 53.0 3.62489 18 0 16 54.0 3.62489 18 0 18 55.0 3.624891 10 0 10 56.0 3.624891 14 0 14 57.0 3.624891 15 0 15 58.0 3.624891 8 0 8 59.0 3.624891 20 0 20 60.0 3.624891 15 0 15 61.0 3.624891 8 0 8 62.0 3.624891 13 0 13 63.0 3.624891 12 0 12 64.0 3.624891 8 0 8 65.0 3.624891 12 0 12 66.0 3.624891 4 0 4 67.0 3.624891 6 0 7 68.0 3.624891 6 0 5 69.0 3.624891 8 0 8 70.0 3.624891 6 0 6 71.0 3.624891 10 0 10 72.0 3.624891 4 0 5 73.0 3.624891 9 0 8 74.0 3.624891 6 0 6 75.0 3.624891 2 0 2 76.0 3.624891 6 0 6 77.0 3.624891 9 0 9 78.0 3.624891 3 0 3 79.0 3.624891 7 0 7 80.0 3.624891 7 0 7 81.0 3.624891 3 0 3 82.0 3.624891 4 0 4 83.0 3.624891 0 0 0 84.0 3.624891 6 0 6 85.0 3.624891 4 0 4 86.0 3.624891 7 0 7 87.0 3.624891 3 0 3 88.0 3.624891 7 0 7 89.0 3.624891 3 0 3 90.0 3.624891 7 0 7 91.0 3.624891 2 0 2 92.0 3.624891 5 0 5 93.0 3.624891 3 0 3 94.0 3.624891 2 0 2 95.0 3.624891 4 0 4 96.0 3.624891 4 0 4 97.0 3.624891 2 0 2 98.0 3.624891 3 0 3 99.0 3.624891 1 0 2 100.0 3.624891 7 0 6 101.0 0 2 0 2 102.0 0 3 0 3 103.0 0 4 0 4 105.0 0 2 0 2 106.0 0 2 0 2 107.0 0 1 0 1 109.0 0 3 0 3 110.0 0 2 0 2 111.0 0 1 0 1 113.0 0 2 0 2 114.0 0 1 0 1 115.0 0 2 0 2 117.0 0 3 0 3 118.0 0 4 0 4 119.0 0 2 0 2 120.0 0 1 0 1 121.0 0 3 0 3 123.0 0 1 0 1 124.0 0 4 0 4 126.0 0 1 0 1 127.0 0 2 0 2 128.0 0 1 0 1 129.0 0 2 0 2 130.0 0 1 0 1 131.0 0 1 0 1 132.0 0 1 0 1 133.0 0 1 0 1 134.0 0 2 0 2 135.0 0 1 0 1 137.0 0 1 0 1 139.0 0 3 0 3 141.0 0 1 0 1 142.0 0 2 0 2 144.0 0 2 0 2 147.0 0 1 0 1 148.0 0 1 0 1 154.0 0 1 0 1 156.0 0 2 0 2 157.0 0 1 0 1 163.0 0 1 0 1 164.0 0 1 0 1 167.0 0 1 0 1 170.0 0 1 0 1 171.0 0 2 0 2 172.0 0 1 0 1 174.0 0 1 0 1 177.0 0 1 0 1 181.0 0 1 0 1 182.0 0 1 0 1 183.0 0 1 0 1 185.0 0 1 0 1 188.0 0 2 0 2 189.0 0 1 0 1 190.0 0 1 0 1 191.0 0 2 0 2 194.0 0 2 0 2 197.0 0 1 0 1 199.0 0 1 0 1 200.0 0 1 0 1 201.0 0 1 0 1 202.0 0 1 0 1 203.0 0 3 0 3 204.0 0 1 0 1 207.0 0 2 0 2 213.0 0 1 0 1 217.0 0 1 0 1 220.0 0 1 0 1 225.0 0 1 0 1 230.0 0 0 0 1 231.0 0 1 0 0 234.0 0 2 0 2 239.0 0 1 0 1 240.0 0 1 0 1 242.0 0 1 0 1 243.0 0 1 0 1 244.0 0 1 0 1 245.0 0 1 0 1 249.0 0 1 0 1 252.0 0 1 0 1 254.0 0 1 0 1 255.0 0 1 0 1 258.0 0 1 0 1 261.0 0 1 0 1 262.0 0 1 0 1 278.0 0 1 0 1 290.0 0 1 0 1 304.0 0 1 0 1 307.0 0 0 0 1 308.0 0 1 0 0 346.0 0 1 0 1 349.0 0 1 0 1 372.0 0 2 0 2 374.0 0 1 0 1 384.0 0 1 0 1 387.0 0 1 0 1 388.0 0 1 0 1 448.0 0 1 0 1 453.0 0 1 0 1 467.0 0 1 0 1 512.0 0 1 0 1 635.0 0 1 0 1 638.0 0 1 0 1 651.0 0 1 0 1