## htsjdk.samtools.metrics.StringHeader # MarkDuplicates MAX_FILE_HANDLES_FOR_READ_ENDS_MAP=999 INPUT=[/ceph/rimlsfnwi/data/moldevbio/heeringen/siebrenf/ghe_2021/results/star/hg38-NP_Day10_supernatant_TA_A71_S7.samtools-coordinate-sieved.bam] OUTPUT=/ceph/rimlsfnwi/data/moldevbio/heeringen/siebrenf/ghe_2021/results/final_bam/hg38-NP_Day10_supernatant_TA_A71_S7.samtools-coordinate.bam METRICS_FILE=/ceph/rimlsfnwi/data/moldevbio/heeringen/siebrenf/ghe_2021/results/qc/markdup/hg38-NP_Day10_supernatant_TA_A71_S7.samtools-coordinate.metrics.txt MAX_SEQUENCES_FOR_DISK_READ_ENDS_MAP=50000 SORTING_COLLECTION_SIZE_RATIO=0.25 TAG_DUPLICATE_SET_MEMBERS=false REMOVE_SEQUENCING_DUPLICATES=false TAGGING_POLICY=DontTag CLEAR_DT=true DUPLEX_UMI=false ADD_PG_TAG_TO_READS=true REMOVE_DUPLICATES=false ASSUME_SORTED=false DUPLICATE_SCORING_STRATEGY=SUM_OF_BASE_QUALITIES PROGRAM_RECORD_ID=MarkDuplicates PROGRAM_GROUP_NAME=MarkDuplicates READ_NAME_REGEX= OPTICAL_DUPLICATE_PIXEL_DISTANCE=100 MAX_OPTICAL_DUPLICATE_SET_SIZE=300000 VERBOSITY=INFO QUIET=false VALIDATION_STRINGENCY=STRICT COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false GA4GH_CLIENT_SECRETS=client_secrets.json USE_JDK_DEFLATER=false USE_JDK_INFLATER=false ## htsjdk.samtools.metrics.StringHeader # Started on: Mon Aug 30 14:11:43 CEST 2021 ## METRICS CLASS picard.sam.DuplicationMetrics LIBRARY UNPAIRED_READS_EXAMINED READ_PAIRS_EXAMINED SECONDARY_OR_SUPPLEMENTARY_RDS UNMAPPED_READS UNPAIRED_READ_DUPLICATES READ_PAIR_DUPLICATES READ_PAIR_OPTICAL_DUPLICATES PERCENT_DUPLICATION ESTIMATED_LIBRARY_SIZE Unknown Library 192 14056657 0 0 91 3333225 3854 0.237129 24760355 ## HISTOGRAM java.lang.Double BIN CoverageMult all_sets optical_sets non_optical_sets 1.0 1.000204 8328809 0 8330966 2.0 1.567142 1909126 3847 1908147 3.0 1.888496 369907 2 369052 4.0 2.070647 70245 1 70018 5.0 2.173894 16972 0 16895 6.0 2.232417 6637 0 6627 7.0 2.265589 3703 0 3699 8.0 2.284392 2436 0 2435 9.0 2.295049 1871 0 1870 10.0 2.30109 1375 0 1378 11.0 2.304515 1119 0 1117 12.0 2.306456 940 0 939 13.0 2.307556 703 0 701 14.0 2.308179 640 0 639 15.0 2.308533 533 0 534 16.0 2.308733 469 0 469 17.0 2.308847 417 0 419 18.0 2.308911 365 0 363 19.0 2.308948 327 0 328 20.0 2.308968 278 0 276 21.0 2.30898 248 0 249 22.0 2.308987 246 0 246 23.0 2.30899 243 0 242 24.0 2.308993 185 0 186 25.0 2.308994 200 0 199 26.0 2.308994 161 0 162 27.0 2.308995 159 0 159 28.0 2.308995 120 0 119 29.0 2.308995 122 0 122 30.0 2.308995 105 0 107 31.0 2.308995 104 0 102 32.0 2.308995 91 0 92 33.0 2.308995 100 0 99 34.0 2.308995 102 0 103 35.0 2.308995 79 0 78 36.0 2.308995 69 0 69 37.0 2.308995 76 0 76 38.0 2.308995 73 0 73 39.0 2.308995 81 0 81 40.0 2.308995 73 0 75 41.0 2.308995 48 0 46 42.0 2.308995 58 0 58 43.0 2.308995 43 0 43 44.0 2.308995 50 0 50 45.0 2.308995 43 0 43 46.0 2.308995 48 0 48 47.0 2.308995 35 0 35 48.0 2.308995 54 0 54 49.0 2.308995 52 0 52 50.0 2.308995 28 0 28 51.0 2.308995 37 0 38 52.0 2.308995 35 0 34 53.0 2.308995 42 0 43 54.0 2.308995 40 0 39 55.0 2.308995 31 0 31 56.0 2.308995 27 0 27 57.0 2.308995 22 0 22 58.0 2.308995 21 0 22 59.0 2.308995 27 0 26 60.0 2.308995 29 0 30 61.0 2.308995 18 0 17 62.0 2.308995 21 0 21 63.0 2.308995 21 0 21 64.0 2.308995 30 0 31 65.0 2.308995 17 0 18 66.0 2.308995 20 0 18 67.0 2.308995 23 0 23 68.0 2.308995 17 0 17 69.0 2.308995 20 0 20 70.0 2.308995 21 0 21 71.0 2.308995 20 0 20 72.0 2.308995 18 0 18 73.0 2.308995 8 0 8 74.0 2.308995 22 0 22 75.0 2.308995 20 0 20 76.0 2.308995 15 0 15 77.0 2.308995 12 0 12 78.0 2.308995 17 0 17 79.0 2.308995 20 0 20 80.0 2.308995 13 0 14 81.0 2.308995 16 0 15 82.0 2.308995 11 0 11 83.0 2.308995 8 0 8 84.0 2.308995 14 0 14 85.0 2.308995 7 0 7 86.0 2.308995 8 0 8 87.0 2.308995 6 0 6 88.0 2.308995 4 0 4 89.0 2.308995 12 0 12 90.0 2.308995 10 0 10 91.0 2.308995 11 0 11 92.0 2.308995 10 0 11 93.0 2.308995 6 0 5 94.0 2.308995 6 0 6 95.0 2.308995 6 0 6 96.0 2.308995 8 0 8 97.0 2.308995 4 0 4 98.0 2.308995 11 0 11 99.0 2.308995 10 0 10 100.0 2.308995 5 0 5 101.0 0 10 0 10 102.0 0 6 0 6 103.0 0 7 0 7 104.0 0 6 0 6 105.0 0 7 0 8 106.0 0 6 0 5 107.0 0 6 0 6 108.0 0 5 0 5 109.0 0 12 0 12 110.0 0 5 0 5 111.0 0 8 0 9 112.0 0 7 0 6 113.0 0 9 0 10 114.0 0 9 0 8 115.0 0 7 0 7 116.0 0 4 0 4 117.0 0 3 0 3 118.0 0 6 0 6 119.0 0 7 0 7 120.0 0 3 0 3 121.0 0 3 0 3 122.0 0 7 0 7 123.0 0 3 0 3 124.0 0 1 0 1 125.0 0 3 0 3 126.0 0 3 0 3 127.0 0 4 0 4 128.0 0 1 0 1 129.0 0 7 0 7 130.0 0 3 0 3 131.0 0 2 0 2 132.0 0 6 0 6 133.0 0 5 0 5 134.0 0 6 0 6 135.0 0 1 0 1 136.0 0 6 0 6 137.0 0 4 0 4 138.0 0 1 0 1 139.0 0 1 0 1 140.0 0 4 0 4 141.0 0 8 0 8 142.0 0 3 0 3 143.0 0 2 0 2 144.0 0 2 0 2 145.0 0 3 0 3 146.0 0 1 0 1 147.0 0 2 0 2 148.0 0 2 0 2 149.0 0 1 0 1 150.0 0 3 0 3 152.0 0 1 0 1 153.0 0 3 0 3 154.0 0 2 0 2 155.0 0 1 0 1 156.0 0 3 0 3 157.0 0 1 0 1 158.0 0 3 0 3 159.0 0 1 0 1 160.0 0 5 0 5 161.0 0 2 0 2 163.0 0 1 0 1 164.0 0 2 0 2 165.0 0 1 0 1 167.0 0 3 0 3 168.0 0 2 0 2 169.0 0 1 0 1 171.0 0 2 0 2 172.0 0 3 0 3 173.0 0 1 0 2 174.0 0 1 0 0 175.0 0 1 0 1 176.0 0 5 0 6 177.0 0 3 0 2 179.0 0 1 0 1 180.0 0 1 0 1 181.0 0 1 0 1 182.0 0 2 0 2 183.0 0 1 0 1 184.0 0 3 0 3 185.0 0 3 0 3 186.0 0 2 0 2 190.0 0 3 0 3 191.0 0 1 0 1 192.0 0 2 0 2 193.0 0 1 0 1 194.0 0 3 0 3 196.0 0 1 0 1 197.0 0 1 0 1 198.0 0 1 0 1 199.0 0 2 0 2 200.0 0 1 0 1 203.0 0 3 0 3 205.0 0 1 0 2 206.0 0 1 0 0 207.0 0 1 0 1 208.0 0 3 0 3 209.0 0 1 0 1 210.0 0 2 0 2 211.0 0 1 0 1 212.0 0 3 0 3 213.0 0 2 0 2 214.0 0 1 0 1 216.0 0 2 0 2 219.0 0 1 0 1 220.0 0 3 0 3 221.0 0 2 0 2 222.0 0 3 0 3 224.0 0 2 0 2 225.0 0 2 0 2 226.0 0 2 0 2 228.0 0 2 0 2 230.0 0 1 0 1 232.0 0 1 0 1 233.0 0 2 0 2 234.0 0 0 0 1 235.0 0 1 0 0 236.0 0 3 0 3 238.0 0 1 0 1 239.0 0 1 0 1 241.0 0 1 0 1 242.0 0 1 0 1 248.0 0 3 0 3 253.0 0 3 0 4 254.0 0 1 0 0 256.0 0 1 0 1 257.0 0 1 0 1 258.0 0 1 0 1 259.0 0 0 0 1 260.0 0 1 0 0 261.0 0 2 0 2 263.0 0 1 0 1 271.0 0 1 0 1 274.0 0 1 0 1 277.0 0 1 0 1 278.0 0 1 0 1 280.0 0 1 0 1 288.0 0 1 0 2 290.0 0 1 0 1 291.0 0 1 0 0 293.0 0 1 0 1 295.0 0 1 0 1 301.0 0 1 0 1 304.0 0 1 0 1 305.0 0 1 0 1 306.0 0 1 0 1 307.0 0 1 0 1 309.0 0 2 0 2 310.0 0 1 0 1 314.0 0 1 0 1 315.0 0 1 0 1 318.0 0 0 0 1 319.0 0 1 0 0 327.0 0 1 0 1 340.0 0 0 0 1 341.0 0 2 0 1 361.0 0 1 0 1 363.0 0 1 0 1 378.0 0 1 0 1 387.0 0 1 0 1 392.0 0 1 0 1 434.0 0 1 0 1 460.0 0 1 0 1 467.0 0 1 0 1 480.0 0 1 0 1 489.0 0 1 0 1 502.0 0 1 0 1 506.0 0 2 0 2 541.0 0 1 0 1 545.0 0 1 0 1 566.0 0 1 0 1 601.0 0 1 0 1 650.0 0 0 0 1 651.0 0 1 0 0 727.0 0 1 0 1 738.0 0 1 0 1 739.0 0 1 0 1 742.0 0 1 0 1 779.0 0 0 0 1 780.0 0 1 0 0 835.0 0 1 0 1 881.0 0 1 0 1 884.0 0 0 0 1 885.0 0 1 0 0 886.0 0 0 0 1 887.0 0 1 0 0 942.0 0 1 0 1