## htsjdk.samtools.metrics.StringHeader # MarkDuplicates MAX_FILE_HANDLES_FOR_READ_ENDS_MAP=999 INPUT=[/ceph/rimlsfnwi/data/moldevbio/heeringen/siebrenf/ghe_2021/results/star/hg38-NP_Day10_supernatant_DMSO_A72_S3.samtools-coordinate-sieved.bam] OUTPUT=/ceph/rimlsfnwi/data/moldevbio/heeringen/siebrenf/ghe_2021/results/final_bam/hg38-NP_Day10_supernatant_DMSO_A72_S3.samtools-coordinate.bam METRICS_FILE=/ceph/rimlsfnwi/data/moldevbio/heeringen/siebrenf/ghe_2021/results/qc/markdup/hg38-NP_Day10_supernatant_DMSO_A72_S3.samtools-coordinate.metrics.txt MAX_SEQUENCES_FOR_DISK_READ_ENDS_MAP=50000 SORTING_COLLECTION_SIZE_RATIO=0.25 TAG_DUPLICATE_SET_MEMBERS=false REMOVE_SEQUENCING_DUPLICATES=false TAGGING_POLICY=DontTag CLEAR_DT=true DUPLEX_UMI=false ADD_PG_TAG_TO_READS=true REMOVE_DUPLICATES=false ASSUME_SORTED=false DUPLICATE_SCORING_STRATEGY=SUM_OF_BASE_QUALITIES PROGRAM_RECORD_ID=MarkDuplicates PROGRAM_GROUP_NAME=MarkDuplicates READ_NAME_REGEX= OPTICAL_DUPLICATE_PIXEL_DISTANCE=100 MAX_OPTICAL_DUPLICATE_SET_SIZE=300000 VERBOSITY=INFO QUIET=false VALIDATION_STRINGENCY=STRICT COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false GA4GH_CLIENT_SECRETS=client_secrets.json USE_JDK_DEFLATER=false USE_JDK_INFLATER=false ## htsjdk.samtools.metrics.StringHeader # Started on: Mon Aug 30 14:09:10 CEST 2021 ## METRICS CLASS picard.sam.DuplicationMetrics LIBRARY UNPAIRED_READS_EXAMINED READ_PAIRS_EXAMINED SECONDARY_OR_SUPPLEMENTARY_RDS UNMAPPED_READS UNPAIRED_READ_DUPLICATES READ_PAIR_DUPLICATES READ_PAIR_OPTICAL_DUPLICATES PERCENT_DUPLICATION ESTIMATED_LIBRARY_SIZE Unknown Library 289 16768487 0 0 139 3806754 5041 0.227021 31129533 ## HISTOGRAM java.lang.Double BIN CoverageMult all_sets optical_sets non_optical_sets 1.0 1.000227 10228885 0 10231726 2.0 1.583884 2159690 5025 2158300 3.0 1.924463 426673 8 425707 4.0 2.123199 87958 0 87618 5.0 2.239166 23519 0 23405 6.0 2.306836 9285 0 9267 7.0 2.346323 5086 0 5080 8.0 2.369365 3215 0 3212 9.0 2.382811 2320 0 2320 10.0 2.390656 1735 0 1733 11.0 2.395234 1298 0 1302 12.0 2.397906 1081 0 1078 13.0 2.399465 856 0 855 14.0 2.400374 720 0 719 15.0 2.400905 606 0 608 16.0 2.401215 495 0 492 17.0 2.401396 418 0 418 18.0 2.401501 374 0 374 19.0 2.401563 329 0 330 20.0 2.401599 295 0 295 21.0 2.40162 284 0 283 22.0 2.401632 234 0 234 23.0 2.401639 223 0 223 24.0 2.401643 214 0 214 25.0 2.401646 194 0 194 26.0 2.401647 163 0 163 27.0 2.401648 152 0 155 28.0 2.401648 135 0 133 29.0 2.401649 135 0 134 30.0 2.401649 125 0 126 31.0 2.401649 111 0 111 32.0 2.401649 99 0 99 33.0 2.401649 97 0 96 34.0 2.401649 94 0 94 35.0 2.401649 59 0 60 36.0 2.401649 77 0 77 37.0 2.401649 65 0 64 38.0 2.401649 63 0 64 39.0 2.401649 49 0 49 40.0 2.401649 74 0 73 41.0 2.401649 51 0 52 42.0 2.401649 75 0 74 43.0 2.401649 48 0 48 44.0 2.401649 54 0 54 45.0 2.401649 44 0 44 46.0 2.401649 32 0 32 47.0 2.401649 35 0 35 48.0 2.401649 38 0 38 49.0 2.401649 31 0 33 50.0 2.401649 41 0 39 51.0 2.401649 36 0 36 52.0 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