## htsjdk.samtools.metrics.StringHeader # MarkDuplicates MAX_FILE_HANDLES_FOR_READ_ENDS_MAP=999 INPUT=[/ceph/rimlsfnwi/data/moldevbio/heeringen/siebrenf/ghe_2021/results/star/GRCh37-NP_Day1_adherentcells_DMSO_A70_S14.samtools-coordinate-sieved.bam] OUTPUT=/ceph/rimlsfnwi/data/moldevbio/heeringen/siebrenf/ghe_2021/results/final_bam/GRCh37-NP_Day1_adherentcells_DMSO_A70_S14.samtools-coordinate.bam METRICS_FILE=/ceph/rimlsfnwi/data/moldevbio/heeringen/siebrenf/ghe_2021/results/qc/markdup/GRCh37-NP_Day1_adherentcells_DMSO_A70_S14.samtools-coordinate.metrics.txt MAX_SEQUENCES_FOR_DISK_READ_ENDS_MAP=50000 SORTING_COLLECTION_SIZE_RATIO=0.25 TAG_DUPLICATE_SET_MEMBERS=false REMOVE_SEQUENCING_DUPLICATES=false TAGGING_POLICY=DontTag CLEAR_DT=true DUPLEX_UMI=false ADD_PG_TAG_TO_READS=true REMOVE_DUPLICATES=false ASSUME_SORTED=false DUPLICATE_SCORING_STRATEGY=SUM_OF_BASE_QUALITIES PROGRAM_RECORD_ID=MarkDuplicates PROGRAM_GROUP_NAME=MarkDuplicates READ_NAME_REGEX= OPTICAL_DUPLICATE_PIXEL_DISTANCE=100 MAX_OPTICAL_DUPLICATE_SET_SIZE=300000 VERBOSITY=INFO QUIET=false VALIDATION_STRINGENCY=STRICT COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false GA4GH_CLIENT_SECRETS=client_secrets.json USE_JDK_DEFLATER=false USE_JDK_INFLATER=false ## htsjdk.samtools.metrics.StringHeader # Started on: Tue Aug 31 19:17:30 CEST 2021 ## METRICS CLASS picard.sam.DuplicationMetrics LIBRARY UNPAIRED_READS_EXAMINED READ_PAIRS_EXAMINED SECONDARY_OR_SUPPLEMENTARY_RDS UNMAPPED_READS UNPAIRED_READ_DUPLICATES READ_PAIR_DUPLICATES READ_PAIR_OPTICAL_DUPLICATES PERCENT_DUPLICATION ESTIMATED_LIBRARY_SIZE Unknown Library 241 17875888 0 0 121 2064244 5341 0.115479 71476150 ## HISTOGRAM java.lang.Double BIN CoverageMult all_sets optical_sets non_optical_sets 1.0 1.000263 14685043 0 14689366 2.0 1.779194 938580 5325 934931 3.0 2.385768 101906 8 101329 4.0 2.858123 29125 0 29054 5.0 3.225958 14303 0 14289 6.0 3.512401 8675 0 8674 7.0 3.735462 5619 0 5616 8.0 3.909165 3976 0 3975 9.0 4.044432 3077 0 3077 10.0 4.149768 2361 0 2357 11.0 4.231796 1904 0 1906 12.0 4.295673 1646 0 1642 13.0 4.345416 1330 0 1330 14.0 4.384152 1112 0 1117 15.0 4.414317 1019 0 1017 16.0 4.437807 864 0 863 17.0 4.456099 753 0 753 18.0 4.470344 667 0 671 19.0 4.481437 570 0 566 20.0 4.490075 542 0 543 21.0 4.496802 446 0 446 22.0 4.50204 478 0 475 23.0 4.50612 385 0 385 24.0 4.509296 372 0 375 25.0 4.51177 309 0 307 26.0 4.513696 301 0 300 27.0 4.515196 291 0 294 28.0 4.516364 268 0 265 29.0 4.517274 234 0 236 30.0 4.517983 221 0 218 31.0 4.518534 217 0 219 32.0 4.518964 185 0 184 33.0 4.519298 192 0 193 34.0 4.519559 167 0 166 35.0 4.519762 165 0 165 36.0 4.51992 147 0 147 37.0 4.520043 130 0 130 38.0 4.520138 145 0 145 39.0 4.520213 118 0 121 40.0 4.520271 123 0 121 41.0 4.520316 110 0 109 42.0 4.520352 108 0 108 43.0 4.520379 99 0 99 44.0 4.5204 100 0 100 45.0 4.520417 109 0 108 46.0 4.52043 82 0 85 47.0 4.52044 97 0 95 48.0 4.520448 84 0 83 49.0 4.520454 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