## htsjdk.samtools.metrics.StringHeader # MarkDuplicates MAX_FILE_HANDLES_FOR_READ_ENDS_MAP=999 INPUT=[/ceph/rimlsfnwi/data/moldevbio/heeringen/siebrenf/ghe_2021/results/star/GRCh37-NP_Day19_supernatant_TA_A62_S5.samtools-coordinate-sieved.bam] OUTPUT=/ceph/rimlsfnwi/data/moldevbio/heeringen/siebrenf/ghe_2021/results/final_bam/GRCh37-NP_Day19_supernatant_TA_A62_S5.samtools-coordinate.bam METRICS_FILE=/ceph/rimlsfnwi/data/moldevbio/heeringen/siebrenf/ghe_2021/results/qc/markdup/GRCh37-NP_Day19_supernatant_TA_A62_S5.samtools-coordinate.metrics.txt MAX_SEQUENCES_FOR_DISK_READ_ENDS_MAP=50000 SORTING_COLLECTION_SIZE_RATIO=0.25 TAG_DUPLICATE_SET_MEMBERS=false REMOVE_SEQUENCING_DUPLICATES=false TAGGING_POLICY=DontTag CLEAR_DT=true DUPLEX_UMI=false ADD_PG_TAG_TO_READS=true REMOVE_DUPLICATES=false ASSUME_SORTED=false DUPLICATE_SCORING_STRATEGY=SUM_OF_BASE_QUALITIES PROGRAM_RECORD_ID=MarkDuplicates PROGRAM_GROUP_NAME=MarkDuplicates READ_NAME_REGEX= OPTICAL_DUPLICATE_PIXEL_DISTANCE=100 MAX_OPTICAL_DUPLICATE_SET_SIZE=300000 VERBOSITY=INFO QUIET=false VALIDATION_STRINGENCY=STRICT COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false GA4GH_CLIENT_SECRETS=client_secrets.json USE_JDK_DEFLATER=false USE_JDK_INFLATER=false ## htsjdk.samtools.metrics.StringHeader # Started on: Tue Aug 31 19:21:12 CEST 2021 ## METRICS CLASS picard.sam.DuplicationMetrics LIBRARY UNPAIRED_READS_EXAMINED READ_PAIRS_EXAMINED SECONDARY_OR_SUPPLEMENTARY_RDS UNMAPPED_READS UNPAIRED_READ_DUPLICATES READ_PAIR_DUPLICATES READ_PAIR_OPTICAL_DUPLICATES PERCENT_DUPLICATION ESTIMATED_LIBRARY_SIZE Unknown Library 145 18467025 0 0 70 2645321 5539 0.143247 58242937 ## HISTOGRAM java.lang.Double BIN CoverageMult all_sets optical_sets non_optical_sets 1.0 1.000255 13894533 0 13898397 2.0 1.728721 1633564 5533 1630955 3.0 2.259249 211594 3 210613 4.0 2.645622 39706 0 39488 5.0 2.92701 13314 0 13268 6.0 3.13194 7062 0 7063 7.0 3.281186 4439 0 4435 8.0 3.389879 3103 0 3098 9.0 3.469038 2213 0 2216 10.0 3.526688 1670 0 1668 11.0 3.568673 1310 0 1309 12.0 3.59925 1028 0 1029 13.0 3.621519 860 0 859 14.0 3.637737 680 0 679 15.0 3.649548 573 0 573 16.0 3.65815 542 0 541 17.0 3.664414 442 0 442 18.0 3.668977 384 0 385 19.0 3.672299 349 0 348 20.0 3.674719 291 0 293 21.0 3.676482 241 0 240 22.0 3.677765 234 0 234 23.0 3.6787 211 0 210 24.0 3.679381 213 0 214 25.0 3.679876 158 0 157 26.0 3.680237 168 0 168 27.0 3.6805 135 0 135 28.0 3.680692 127 0 128 29.0 3.680831 131 0 130 30.0 3.680933 109 0 111 31.0 3.681007 112 0 111 32.0 3.681061 111 0 111 33.0 3.6811 91 0 90 34.0 3.681128 82 0 84 35.0 3.681149 80 0 79 36.0 3.681164 71 0 70 37.0 3.681175 77 0 77 38.0 3.681184 59 0 59 39.0 3.681189 67 0 67 40.0 3.681194 53 0 53 41.0 3.681197 59 0 61 42.0 3.681199 46 0 44 43.0 3.681201 40 0 40 44.0 3.681202 54 0 55 45.0 3.681203 42 0 42 46.0 3.681203 30 0 29 47.0 3.681204 29 0 29 48.0 3.681204 40 0 40 49.0 3.681204 29 0 30 50.0 3.681205 28 0 27 51.0 3.681205 29 0 30 52.0 3.681205 33 0 32 53.0 3.681205 24 0 25 54.0 3.681205 33 0 32 55.0 3.681205 17 0 17 56.0 3.681205 25 0 25 57.0 3.681205 18 0 18 58.0 3.681205 23 0 24 59.0 3.681205 21 0 20 60.0 3.681205 25 0 25 61.0 3.681205 27 0 27 62.0 3.681205 21 0 21 63.0 3.681205 21 0 22 64.0 3.681205 18 0 17 65.0 3.681205 23 0 23 66.0 3.681205 23 0 23 67.0 3.681205 17 0 17 68.0 3.681205 14 0 14 69.0 3.681205 12 0 12 70.0 3.681205 16 0 16 71.0 3.681205 8 0 8 72.0 3.681205 16 0 16 73.0 3.681205 14 0 14 74.0 3.681205 17 0 17 75.0 3.681205 8 0 8 76.0 3.681205 10 0 10 77.0 3.681205 12 0 12 78.0 3.681205 10 0 10 79.0 3.681205 9 0 9 80.0 3.681205 9 0 9 81.0 3.681205 10 0 10 82.0 3.681205 11 0 11 83.0 3.681205 8 0 8 84.0 3.681205 8 0 9 85.0 3.681205 16 0 15 86.0 3.681205 9 0 10 87.0 3.681205 13 0 12 88.0 3.681205 12 0 12 89.0 3.681205 10 0 11 90.0 3.681205 3 0 3 91.0 3.681205 9 0 8 92.0 3.681205 8 0 8 93.0 3.681205 5 0 5 94.0 3.681205 9 0 9 95.0 3.681205 2 0 2 96.0 3.681205 5 0 5 97.0 3.681205 7 0 7 98.0 3.681205 6 0 6 99.0 3.681205 10 0 10 100.0 3.681205 4 0 4 101.0 0 7 0 7 102.0 0 8 0 8 103.0 0 9 0 9 104.0 0 6 0 6 105.0 0 7 0 7 106.0 0 8 0 8 107.0 0 8 0 8 108.0 0 1 0 1 109.0 0 1 0 1 110.0 0 1 0 1 111.0 0 3 0 3 112.0 0 4 0 4 113.0 0 4 0 4 114.0 0 6 0 6 115.0 0 3 0 3 116.0 0 1 0 2 117.0 0 5 0 4 118.0 0 3 0 3 119.0 0 6 0 6 120.0 0 2 0 2 121.0 0 4 0 4 122.0 0 3 0 3 123.0 0 5 0 5 124.0 0 5 0 5 125.0 0 4 0 4 126.0 0 2 0 2 127.0 0 7 0 7 128.0 0 5 0 5 129.0 0 5 0 5 130.0 0 2 0 2 131.0 0 5 0 5 132.0 0 2 0 3 133.0 0 2 0 1 134.0 0 1 0 1 135.0 0 3 0 3 136.0 0 3 0 3 137.0 0 3 0 3 138.0 0 1 0 1 139.0 0 4 0 4 140.0 0 1 0 1 141.0 0 2 0 2 142.0 0 4 0 4 143.0 0 2 0 2 144.0 0 2 0 2 145.0 0 2 0 2 146.0 0 3 0 4 147.0 0 5 0 4 148.0 0 3 0 3 149.0 0 4 0 4 150.0 0 3 0 3 151.0 0 2 0 2 152.0 0 4 0 4 153.0 0 3 0 3 154.0 0 1 0 1 155.0 0 1 0 1 156.0 0 1 0 1 157.0 0 1 0 1 158.0 0 1 0 1 159.0 0 4 0 4 160.0 0 2 0 2 161.0 0 5 0 5 162.0 0 4 0 4 163.0 0 1 0 1 164.0 0 3 0 3 165.0 0 1 0 1 166.0 0 3 0 3 167.0 0 1 0 1 168.0 0 1 0 1 169.0 0 4 0 4 170.0 0 4 0 4 171.0 0 1 0 1 173.0 0 2 0 2 175.0 0 2 0 3 176.0 0 1 0 0 179.0 0 1 0 1 180.0 0 2 0 2 181.0 0 1 0 1 182.0 0 3 0 3 183.0 0 3 0 3 184.0 0 2 0 2 185.0 0 1 0 1 186.0 0 1 0 1 187.0 0 2 0 2 188.0 0 1 0 1 189.0 0 1 0 1 190.0 0 1 0 2 191.0 0 1 0 0 192.0 0 1 0 1 193.0 0 2 0 2 194.0 0 1 0 1 195.0 0 1 0 1 196.0 0 1 0 1 197.0 0 1 0 1 198.0 0 0 0 1 199.0 0 1 0 0 200.0 0 5 0 5 201.0 0 2 0 2 202.0 0 3 0 4 203.0 0 1 0 0 204.0 0 1 0 1 205.0 0 2 0 2 206.0 0 5 0 5 207.0 0 1 0 1 208.0 0 5 0 5 209.0 0 1 0 1 211.0 0 2 0 2 212.0 0 1 0 1 213.0 0 2 0 2 214.0 0 1 0 1 215.0 0 2 0 2 216.0 0 1 0 2 217.0 0 1 0 0 218.0 0 1 0 1 219.0 0 2 0 2 220.0 0 3 0 3 221.0 0 2 0 2 223.0 0 1 0 1 224.0 0 1 0 1 228.0 0 2 0 2 229.0 0 1 0 1 232.0 0 2 0 2 235.0 0 2 0 2 237.0 0 1 0 1 238.0 0 1 0 1 239.0 0 2 0 2 240.0 0 1 0 1 241.0 0 1 0 1 242.0 0 1 0 1 243.0 0 2 0 2 244.0 0 1 0 2 246.0 0 1 0 0 251.0 0 1 0 1 253.0 0 1 0 1 256.0 0 1 0 1 260.0 0 1 0 2 261.0 0 2 0 1 262.0 0 1 0 1 270.0 0 1 0 1 274.0 0 1 0 1 276.0 0 2 0 2 277.0 0 2 0 2 279.0 0 1 0 1 280.0 0 1 0 1 283.0 0 1 0 1 284.0 0 1 0 1 285.0 0 1 0 1 293.0 0 1 0 1 297.0 0 1 0 1 304.0 0 1 0 1 308.0 0 1 0 1 318.0 0 1 0 2 319.0 0 1 0 0 322.0 0 1 0 1 325.0 0 1 0 1 333.0 0 1 0 1 335.0 0 1 0 1 336.0 0 1 0 1 343.0 0 1 0 1 348.0 0 1 0 1 351.0 0 1 0 1 354.0 0 1 0 1 359.0 0 1 0 1 365.0 0 1 0 1 370.0 0 1 0 1 371.0 0 1 0 1 379.0 0 1 0 1 390.0 0 0 0 1 391.0 0 1 0 0 401.0 0 1 0 1 419.0 0 1 0 1 423.0 0 1 0 1 446.0 0 1 0 1 467.0 0 1 0 1 470.0 0 0 0 1 471.0 0 1 0 0 490.0 0 3 0 3 495.0 0 1 0 1 499.0 0 1 0 1 510.0 0 1 0 1 529.0 0 1 0 1 564.0 0 1 0 1 596.0 0 1 0 1 601.0 0 0 0 1 603.0 0 1 0 0 655.0 0 1 0 1 689.0 0 1 0 1 720.0 0 2 0 2 721.0 0 1 0 1 749.0 0 1 0 1 762.0 0 1 0 1 842.0 0 1 0 1 859.0 0 1 0 1 926.0 0 1 0 1 1149.0 0 1 0 1 1172.0 0 1 0 1