## htsjdk.samtools.metrics.StringHeader # MarkDuplicates MAX_FILE_HANDLES_FOR_READ_ENDS_MAP=999 INPUT=[/ceph/rimlsfnwi/data/moldevbio/heeringen/siebrenf/ghe_2021/results/star/GRCh37-NP_Day19_supernatant_DMSO_A63_S6.samtools-coordinate-sieved.bam] OUTPUT=/ceph/rimlsfnwi/data/moldevbio/heeringen/siebrenf/ghe_2021/results/final_bam/GRCh37-NP_Day19_supernatant_DMSO_A63_S6.samtools-coordinate.bam METRICS_FILE=/ceph/rimlsfnwi/data/moldevbio/heeringen/siebrenf/ghe_2021/results/qc/markdup/GRCh37-NP_Day19_supernatant_DMSO_A63_S6.samtools-coordinate.metrics.txt MAX_SEQUENCES_FOR_DISK_READ_ENDS_MAP=50000 SORTING_COLLECTION_SIZE_RATIO=0.25 TAG_DUPLICATE_SET_MEMBERS=false REMOVE_SEQUENCING_DUPLICATES=false TAGGING_POLICY=DontTag CLEAR_DT=true DUPLEX_UMI=false ADD_PG_TAG_TO_READS=true REMOVE_DUPLICATES=false ASSUME_SORTED=false DUPLICATE_SCORING_STRATEGY=SUM_OF_BASE_QUALITIES PROGRAM_RECORD_ID=MarkDuplicates PROGRAM_GROUP_NAME=MarkDuplicates READ_NAME_REGEX= OPTICAL_DUPLICATE_PIXEL_DISTANCE=100 MAX_OPTICAL_DUPLICATE_SET_SIZE=300000 VERBOSITY=INFO QUIET=false VALIDATION_STRINGENCY=STRICT COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false GA4GH_CLIENT_SECRETS=client_secrets.json USE_JDK_DEFLATER=false USE_JDK_INFLATER=false ## htsjdk.samtools.metrics.StringHeader # Started on: Tue Aug 31 19:16:51 CEST 2021 ## METRICS CLASS picard.sam.DuplicationMetrics LIBRARY UNPAIRED_READS_EXAMINED READ_PAIRS_EXAMINED SECONDARY_OR_SUPPLEMENTARY_RDS UNMAPPED_READS UNPAIRED_READ_DUPLICATES READ_PAIR_DUPLICATES READ_PAIR_OPTICAL_DUPLICATES PERCENT_DUPLICATION ESTIMATED_LIBRARY_SIZE Unknown Library 157 20393369 0 0 52 2004513 5042 0.098293 97035053 ## HISTOGRAM java.lang.Double BIN CoverageMult all_sets optical_sets non_optical_sets 1.0 1.000222 17054196 0 17058312 2.0 1.810853 1156155 5036 1152753 3.0 2.467829 109789 3 109185 4.0 3.000275 26110 0 26028 5.0 3.431797 11910 0 11899 6.0 3.781524 7026 0 7014 7.0 4.06496 4567 0 4568 8.0 4.294672 3038 0 3033 9.0 4.480841 2275 0 2277 10.0 4.631722 1849 0 1850 11.0 4.754004 1315 0 1314 12.0 4.853107 1083 0 1082 13.0 4.933426 889 0 888 14.0 4.99852 762 0 761 15.0 5.051275 625 0 626 16.0 5.094031 524 0 523 17.0 5.128682 432 0 432 18.0 5.156766 396 0 396 19.0 5.179526 388 0 389 20.0 5.197972 340 0 340 21.0 5.212921 283 0 282 22.0 5.225037 290 0 290 23.0 5.234856 247 0 248 24.0 5.242814 184 0 184 25.0 5.249264 197 0 196 26.0 5.254491 173 0 173 27.0 5.258727 157 0 157 28.0 5.26216 182 0 183 29.0 5.264943 146 0 147 30.0 5.267198 147 0 145 31.0 5.269026 140 0 140 32.0 5.270507 133 0 134 33.0 5.271707 121 0 120 34.0 5.27268 98 0 98 35.0 5.273469 109 0 110 36.0 5.274108 105 0 105 37.0 5.274626 79 0 79 38.0 5.275045 79 0 78 39.0 5.275386 60 0 61 40.0 5.275661 72 0 72 41.0 5.275885 74 0 73 42.0 5.276066 76 0 77 43.0 5.276213 56 0 55 44.0 5.276332 69 0 69 45.0 5.276428 57 0 57 46.0 5.276506 51 0 52 47.0 5.276569 50 0 49 48.0 5.276621 50 0 50 49.0 5.276662 53 0 53 50.0 5.276696 48 0 48 51.0 5.276723 49 0 49 52.0 5.276745 40 0 41 53.0 5.276763 39 0 38 54.0 5.276778 41 0 41 55.0 5.27679 39 0 39 56.0 5.276799 44 0 44 57.0 5.276807 35 0 35 58.0 5.276813 36 0 36 59.0 5.276818 26 0 27 60.0 5.276822 30 0 30 61.0 5.276826 38 0 37 62.0 5.276829 29 0 29 63.0 5.276831 14 0 14 64.0 5.276833 27 0 27 65.0 5.276834 24 0 24 66.0 5.276835 25 0 25 67.0 5.276836 27 0 27 68.0 5.276837 26 0 26 69.0 5.276837 16 0 16 70.0 5.276838 23 0 23 71.0 5.276838 20 0 20 72.0 5.276839 10 0 10 73.0 5.276839 17 0 17 74.0 5.276839 18 0 18 75.0 5.276839 15 0 15 76.0 5.27684 21 0 21 77.0 5.27684 22 0 22 78.0 5.27684 10 0 10 79.0 5.27684 9 0 9 80.0 5.27684 16 0 16 81.0 5.27684 24 0 24 82.0 5.27684 9 0 9 83.0 5.27684 10 0 10 84.0 5.27684 7 0 7 85.0 5.27684 14 0 14 86.0 5.27684 12 0 12 87.0 5.27684 16 0 16 88.0 5.27684 10 0 10 89.0 5.27684 11 0 11 90.0 5.27684 9 0 9 91.0 5.27684 15 0 15 92.0 5.27684 8 0 8 93.0 5.27684 10 0 10 94.0 5.27684 10 0 10 95.0 5.27684 6 0 6 96.0 5.27684 6 0 6 97.0 5.27684 15 0 15 98.0 5.27684 4 0 4 99.0 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