## htsjdk.samtools.metrics.StringHeader # MarkDuplicates MAX_FILE_HANDLES_FOR_READ_ENDS_MAP=999 INPUT=[/ceph/rimlsfnwi/data/moldevbio/heeringen/siebrenf/ghe_2021/results/star/GRCh37-NP_Day12_supernatant_TA_A73_S10.samtools-coordinate-sieved.bam] OUTPUT=/ceph/rimlsfnwi/data/moldevbio/heeringen/siebrenf/ghe_2021/results/final_bam/GRCh37-NP_Day12_supernatant_TA_A73_S10.samtools-coordinate.bam METRICS_FILE=/ceph/rimlsfnwi/data/moldevbio/heeringen/siebrenf/ghe_2021/results/qc/markdup/GRCh37-NP_Day12_supernatant_TA_A73_S10.samtools-coordinate.metrics.txt MAX_SEQUENCES_FOR_DISK_READ_ENDS_MAP=50000 SORTING_COLLECTION_SIZE_RATIO=0.25 TAG_DUPLICATE_SET_MEMBERS=false REMOVE_SEQUENCING_DUPLICATES=false TAGGING_POLICY=DontTag CLEAR_DT=true DUPLEX_UMI=false ADD_PG_TAG_TO_READS=true REMOVE_DUPLICATES=false ASSUME_SORTED=false DUPLICATE_SCORING_STRATEGY=SUM_OF_BASE_QUALITIES PROGRAM_RECORD_ID=MarkDuplicates PROGRAM_GROUP_NAME=MarkDuplicates READ_NAME_REGEX= OPTICAL_DUPLICATE_PIXEL_DISTANCE=100 MAX_OPTICAL_DUPLICATE_SET_SIZE=300000 VERBOSITY=INFO QUIET=false VALIDATION_STRINGENCY=STRICT COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false GA4GH_CLIENT_SECRETS=client_secrets.json USE_JDK_DEFLATER=false USE_JDK_INFLATER=false ## htsjdk.samtools.metrics.StringHeader # Started on: Tue Aug 31 19:22:41 CEST 2021 ## METRICS CLASS picard.sam.DuplicationMetrics LIBRARY UNPAIRED_READS_EXAMINED READ_PAIRS_EXAMINED SECONDARY_OR_SUPPLEMENTARY_RDS UNMAPPED_READS UNPAIRED_READ_DUPLICATES READ_PAIR_DUPLICATES READ_PAIR_OPTICAL_DUPLICATES PERCENT_DUPLICATION ESTIMATED_LIBRARY_SIZE Unknown Library 278 17348780 0 0 158 2225669 5273 0.128293 61821301 ## HISTOGRAM java.lang.Double BIN CoverageMult all_sets optical_sets non_optical_sets 1.0 1.000263 13647354 0 13651376 2.0 1.755772 1228884 5269 1225803 3.0 2.326414 150751 2 149944 4.0 2.757426 39012 0 38917 5.0 3.082973 17597 0 17580 6.0 3.328862 10068 0 10064 7.0 3.514584 6508 0 6498 8.0 3.654861 4378 0 4379 9.0 3.760814 3152 0 3155 10.0 3.840842 2342 0 2340 11.0 3.901287 1769 0 1762 12.0 3.946942 1376 0 1379 13.0 3.981426 1089 0 1086 14.0 4.007471 934 0 932 15.0 4.027144 895 0 896 16.0 4.042003 695 0 694 17.0 4.053226 535 0 537 18.0 4.061703 503 0 502 19.0 4.068106 389 0 388 20.0 4.072942 325 0 324 21.0 4.076594 312 0 314 22.0 4.079353 258 0 256 23.0 4.081437 247 0 247 24.0 4.083011 247 0 248 25.0 4.0842 207 0 207 26.0 4.085098 196 0 196 27.0 4.085776 176 0 176 28.0 4.086288 166 0 165 29.0 4.086675 152 0 152 30.0 4.086967 124 0 125 31.0 4.087188 119 0 119 32.0 4.087355 126 0 125 33.0 4.087481 109 0 109 34.0 4.087576 92 0 93 35.0 4.087648 87 0 86 36.0 4.087702 82 0 83 37.0 4.087743 89 0 88 38.0 4.087774 62 0 62 39.0 4.087797 58 0 58 40.0 4.087815 62 0 62 41.0 4.087828 54 0 55 42.0 4.087838 55 0 55 43.0 4.087846 46 0 45 44.0 4.087852 44 0 44 45.0 4.087856 47 0 48 46.0 4.087859 50 0 49 47.0 4.087862 40 0 40 48.0 4.087864 37 0 38 49.0 4.087865 33 0 32 50.0 4.087866 35 0 35 51.0 4.087867 43 0 43 52.0 4.087867 33 0 33 53.0 4.087868 29 0 31 54.0 4.087868 33 0 31 55.0 4.087868 23 0 24 56.0 4.087869 30 0 29 57.0 4.087869 22 0 23 58.0 4.087869 29 0 28 59.0 4.087869 19 0 20 60.0 4.087869 24 0 23 61.0 4.087869 26 0 27 62.0 4.087869 23 0 22 63.0 4.087869 14 0 14 64.0 4.087869 23 0 23 65.0 4.087869 26 0 26 66.0 4.087869 10 0 10 67.0 4.087869 11 0 11 68.0 4.087869 13 0 14 69.0 4.087869 17 0 16 70.0 4.087869 13 0 13 71.0 4.087869 17 0 17 72.0 4.087869 11 0 11 73.0 4.087869 14 0 14 74.0 4.087869 10 0 10 75.0 4.087869 10 0 10 76.0 4.087869 15 0 15 77.0 4.087869 9 0 9 78.0 4.087869 11 0 11 79.0 4.087869 10 0 10 80.0 4.087869 11 0 11 81.0 4.087869 15 0 15 82.0 4.087869 14 0 14 83.0 4.087869 10 0 10 84.0 4.087869 6 0 6 85.0 4.087869 5 0 5 86.0 4.087869 8 0 8 87.0 4.087869 7 0 7 88.0 4.087869 9 0 9 89.0 4.087869 9 0 9 90.0 4.087869 8 0 8 91.0 4.087869 5 0 5 92.0 4.087869 13 0 13 93.0 4.087869 10 0 10 94.0 4.087869 15 0 15 95.0 4.087869 8 0 8 96.0 4.087869 4 0 4 97.0 4.087869 4 0 4 98.0 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174.0 0 1 0 1 175.0 0 2 0 3 176.0 0 3 0 2 177.0 0 2 0 2 178.0 0 1 0 1 180.0 0 2 0 2 181.0 0 3 0 3 183.0 0 4 0 4 187.0 0 1 0 1 188.0 0 2 0 2 191.0 0 2 0 2 192.0 0 3 0 3 193.0 0 3 0 3 194.0 0 2 0 2 195.0 0 1 0 1 197.0 0 2 0 2 202.0 0 1 0 1 203.0 0 3 0 3 204.0 0 2 0 2 206.0 0 2 0 2 207.0 0 1 0 1 208.0 0 1 0 1 210.0 0 2 0 2 211.0 0 1 0 1 214.0 0 1 0 1 218.0 0 2 0 2 219.0 0 3 0 3 222.0 0 1 0 1 223.0 0 1 0 1 224.0 0 2 0 2 225.0 0 1 0 1 229.0 0 2 0 2 232.0 0 1 0 1 233.0 0 2 0 2 234.0 0 1 0 1 237.0 0 1 0 1 241.0 0 0 0 1 242.0 0 2 0 1 249.0 0 1 0 1 250.0 0 1 0 1 251.0 0 1 0 1 253.0 0 2 0 2 255.0 0 2 0 2 258.0 0 1 0 1 265.0 0 0 0 1 266.0 0 1 0 0 270.0 0 1 0 1 273.0 0 1 0 1 275.0 0 1 0 1 277.0 0 1 0 1 278.0 0 1 0 1 283.0 0 1 0 1 284.0 0 1 0 1 285.0 0 1 0 1 286.0 0 1 0 1 289.0 0 1 0 1 290.0 0 2 0 2 291.0 0 1 0 1 294.0 0 1 0 1 305.0 0 1 0 1 309.0 0 1 0 1 314.0 0 2 0 2 315.0 0 1 0 1 317.0 0 1 0 1 320.0 0 1 0 1 327.0 0 1 0 1 330.0 0 1 0 1 332.0 0 1 0 1 336.0 0 1 0 1 345.0 0 1 0 1 348.0 0 1 0 1 349.0 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