## htsjdk.samtools.metrics.StringHeader # MarkDuplicates MAX_FILE_HANDLES_FOR_READ_ENDS_MAP=999 INPUT=[/ceph/rimlsfnwi/data/moldevbio/heeringen/siebrenf/ghe_2021/results/star/GRCh37-NP_Day12_adherent_TA_A60_S4.samtools-coordinate-sieved.bam] OUTPUT=/ceph/rimlsfnwi/data/moldevbio/heeringen/siebrenf/ghe_2021/results/final_bam/GRCh37-NP_Day12_adherent_TA_A60_S4.samtools-coordinate.bam METRICS_FILE=/ceph/rimlsfnwi/data/moldevbio/heeringen/siebrenf/ghe_2021/results/qc/markdup/GRCh37-NP_Day12_adherent_TA_A60_S4.samtools-coordinate.metrics.txt MAX_SEQUENCES_FOR_DISK_READ_ENDS_MAP=50000 SORTING_COLLECTION_SIZE_RATIO=0.25 TAG_DUPLICATE_SET_MEMBERS=false REMOVE_SEQUENCING_DUPLICATES=false TAGGING_POLICY=DontTag CLEAR_DT=true DUPLEX_UMI=false ADD_PG_TAG_TO_READS=true REMOVE_DUPLICATES=false ASSUME_SORTED=false DUPLICATE_SCORING_STRATEGY=SUM_OF_BASE_QUALITIES PROGRAM_RECORD_ID=MarkDuplicates PROGRAM_GROUP_NAME=MarkDuplicates READ_NAME_REGEX= OPTICAL_DUPLICATE_PIXEL_DISTANCE=100 MAX_OPTICAL_DUPLICATE_SET_SIZE=300000 VERBOSITY=INFO QUIET=false VALIDATION_STRINGENCY=STRICT COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false GA4GH_CLIENT_SECRETS=client_secrets.json USE_JDK_DEFLATER=false USE_JDK_INFLATER=false ## htsjdk.samtools.metrics.StringHeader # Started on: Tue Aug 31 19:21:17 CEST 2021 ## METRICS CLASS picard.sam.DuplicationMetrics LIBRARY UNPAIRED_READS_EXAMINED READ_PAIRS_EXAMINED SECONDARY_OR_SUPPLEMENTARY_RDS UNMAPPED_READS UNPAIRED_READ_DUPLICATES READ_PAIR_DUPLICATES READ_PAIR_OPTICAL_DUPLICATES PERCENT_DUPLICATION ESTIMATED_LIBRARY_SIZE Unknown Library 227 18660132 0 0 145 2736578 6592 0.146657 57345620 ## HISTOGRAM java.lang.Double BIN CoverageMult all_sets optical_sets non_optical_sets 1.0 1.000299 13923978 0 13928605 2.0 1.722755 1649015 6578 1645797 3.0 2.244542 241544 7 240470 4.0 2.621397 53580 0 53336 5.0 2.893577 19800 0 19738 6.0 3.090156 10537 0 10525 7.0 3.232134 6310 0 6305 8.0 3.334675 4145 0 4137 9.0 3.408735 2913 0 2918 10.0 3.462224 2115 0 2111 11.0 3.500856 1648 0 1648 12.0 3.528758 1167 0 1165 13.0 3.548909 947 0 948 14.0 3.563463 776 0 776 15.0 3.573975 610 0 609 16.0 3.581567 515 0 515 17.0 3.58705 392 0 392 18.0 3.59101 337 0 335 19.0 3.593871 331 0 330 20.0 3.595936 247 0 249 21.0 3.597428 216 0 216 22.0 3.598506 208 0 208 23.0 3.599284 184 0 184 24.0 3.599846 159 0 157 25.0 3.600252 143 0 144 26.0 3.600545 111 0 111 27.0 3.600757 98 0 99 28.0 3.60091 109 0 107 29.0 3.601021 78 0 78 30.0 3.6011 65 0 66 31.0 3.601158 75 0 77 32.0 3.6012 70 0 68 33.0 3.60123 55 0 56 34.0 3.601251 58 0 56 35.0 3.601267 54 0 54 36.0 3.601278 41 0 42 37.0 3.601287 49 0 48 38.0 3.601292 42 0 42 39.0 3.601297 41 0 41 40.0 3.6013 22 0 22 41.0 3.601302 32 0 32 42.0 3.601304 23 0 23 43.0 3.601305 33 0 33 44.0 3.601306 29 0 29 45.0 3.601306 17 0 17 46.0 3.601307 28 0 28 47.0 3.601307 34 0 35 48.0 3.601307 18 0 17 49.0 3.601307 17 0 17 50.0 3.601308 19 0 19 51.0 3.601308 20 0 20 52.0 3.601308 12 0 14 53.0 3.601308 18 0 16 54.0 3.601308 18 0 18 55.0 3.601308 10 0 10 56.0 3.601308 14 0 14 57.0 3.601308 16 0 16 58.0 3.601308 8 0 8 59.0 3.601308 20 0 20 60.0 3.601308 15 0 15 61.0 3.601308 8 0 8 62.0 3.601308 13 0 13 63.0 3.601308 12 0 12 64.0 3.601308 9 0 9 65.0 3.601308 12 0 12 66.0 3.601308 4 0 4 67.0 3.601308 6 0 7 68.0 3.601308 6 0 5 69.0 3.601308 8 0 8 70.0 3.601308 6 0 6 71.0 3.601308 10 0 10 72.0 3.601308 4 0 5 73.0 3.601308 9 0 8 74.0 3.601308 6 0 6 75.0 3.601308 2 0 2 76.0 3.601308 6 0 6 77.0 3.601308 10 0 10 78.0 3.601308 3 0 3 79.0 3.601308 7 0 7 80.0 3.601308 7 0 7 81.0 3.601308 3 0 3 82.0 3.601308 4 0 4 83.0 3.601308 0 0 0 84.0 3.601308 6 0 6 85.0 3.601308 4 0 4 86.0 3.601308 7 0 7 87.0 3.601308 3 0 3 88.0 3.601308 7 0 7 89.0 3.601308 3 0 3 90.0 3.601308 7 0 7 91.0 3.601308 2 0 2 92.0 3.601308 5 0 5 93.0 3.601308 3 0 3 94.0 3.601308 2 0 2 95.0 3.601308 4 0 4 96.0 3.601308 4 0 4 97.0 3.601308 2 0 2 98.0 3.601308 3 0 3 99.0 3.601308 2 0 3 100.0 3.601308 7 0 6 101.0 0 2 0 2 102.0 0 3 0 3 103.0 0 4 0 4 105.0 0 2 0 2 106.0 0 2 0 2 107.0 0 1 0 1 109.0 0 3 0 3 110.0 0 2 0 2 111.0 0 1 0 1 113.0 0 2 0 2 114.0 0 1 0 1 115.0 0 2 0 2 117.0 0 3 0 3 118.0 0 4 0 4 119.0 0 2 0 2 120.0 0 1 0 1 121.0 0 3 0 3 123.0 0 1 0 1 124.0 0 4 0 4 126.0 0 1 0 1 127.0 0 2 0 2 128.0 0 1 0 1 129.0 0 2 0 2 130.0 0 1 0 1 131.0 0 1 0 1 132.0 0 1 0 1 133.0 0 1 0 1 134.0 0 2 0 2 135.0 0 1 0 1 137.0 0 1 0 1 139.0 0 3 0 3 141.0 0 1 0 1 142.0 0 2 0 2 144.0 0 2 0 2 147.0 0 1 0 1 148.0 0 1 0 1 154.0 0 1 0 1 156.0 0 2 0 2 157.0 0 1 0 1 163.0 0 1 0 1 164.0 0 1 0 1 167.0 0 1 0 1 170.0 0 1 0 1 171.0 0 2 0 2 172.0 0 1 0 1 174.0 0 1 0 1 177.0 0 1 0 1 181.0 0 1 0 1 182.0 0 1 0 1 183.0 0 1 0 1 185.0 0 1 0 1 188.0 0 2 0 2 189.0 0 1 0 1 190.0 0 1 0 1 191.0 0 2 0 2 194.0 0 2 0 2 197.0 0 1 0 1 199.0 0 1 0 1 200.0 0 1 0 1 201.0 0 1 0 1 202.0 0 1 0 1 203.0 0 3 0 3 204.0 0 1 0 1 207.0 0 2 0 2 213.0 0 1 0 1 217.0 0 1 0 1 220.0 0 1 0 1 225.0 0 1 0 1 230.0 0 0 0 1 231.0 0 1 0 0 234.0 0 2 0 2 239.0 0 1 0 1 240.0 0 1 0 1 242.0 0 1 0 1 243.0 0 1 0 1 244.0 0 1 0 1 245.0 0 1 0 1 249.0 0 1 0 1 252.0 0 1 0 1 254.0 0 1 0 1 255.0 0 1 0 1 258.0 0 1 0 1 261.0 0 1 0 1 262.0 0 1 0 1 278.0 0 1 0 1 290.0 0 1 0 1 304.0 0 1 0 1 307.0 0 0 0 1 308.0 0 1 0 0 346.0 0 1 0 1 349.0 0 1 0 1 372.0 0 2 0 2 374.0 0 1 0 1 384.0 0 1 0 1 387.0 0 1 0 1 388.0 0 1 0 1 448.0 0 1 0 1 453.0 0 1 0 1 467.0 0 1 0 1 512.0 0 1 0 1 635.0 0 1 0 1 638.0 0 1 0 1 651.0 0 1 0 1