## htsjdk.samtools.metrics.StringHeader # MarkDuplicates MAX_FILE_HANDLES_FOR_READ_ENDS_MAP=999 INPUT=[/ceph/rimlsfnwi/data/moldevbio/heeringen/siebrenf/ghe_2021/results/star/GRCh37-NP_Day12_adherent_DMSO_A61_S9.samtools-coordinate-sieved.bam] OUTPUT=/ceph/rimlsfnwi/data/moldevbio/heeringen/siebrenf/ghe_2021/results/final_bam/GRCh37-NP_Day12_adherent_DMSO_A61_S9.samtools-coordinate.bam METRICS_FILE=/ceph/rimlsfnwi/data/moldevbio/heeringen/siebrenf/ghe_2021/results/qc/markdup/GRCh37-NP_Day12_adherent_DMSO_A61_S9.samtools-coordinate.metrics.txt MAX_SEQUENCES_FOR_DISK_READ_ENDS_MAP=50000 SORTING_COLLECTION_SIZE_RATIO=0.25 TAG_DUPLICATE_SET_MEMBERS=false REMOVE_SEQUENCING_DUPLICATES=false TAGGING_POLICY=DontTag CLEAR_DT=true DUPLEX_UMI=false ADD_PG_TAG_TO_READS=true REMOVE_DUPLICATES=false ASSUME_SORTED=false DUPLICATE_SCORING_STRATEGY=SUM_OF_BASE_QUALITIES PROGRAM_RECORD_ID=MarkDuplicates PROGRAM_GROUP_NAME=MarkDuplicates READ_NAME_REGEX= OPTICAL_DUPLICATE_PIXEL_DISTANCE=100 MAX_OPTICAL_DUPLICATE_SET_SIZE=300000 VERBOSITY=INFO QUIET=false VALIDATION_STRINGENCY=STRICT COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false GA4GH_CLIENT_SECRETS=client_secrets.json USE_JDK_DEFLATER=false USE_JDK_INFLATER=false ## htsjdk.samtools.metrics.StringHeader # Started on: Tue Aug 31 19:19:03 CEST 2021 ## METRICS CLASS picard.sam.DuplicationMetrics LIBRARY UNPAIRED_READS_EXAMINED READ_PAIRS_EXAMINED SECONDARY_OR_SUPPLEMENTARY_RDS UNMAPPED_READS UNPAIRED_READ_DUPLICATES READ_PAIR_DUPLICATES READ_PAIR_OPTICAL_DUPLICATES PERCENT_DUPLICATION ESTIMATED_LIBRARY_SIZE Unknown Library 264 27811283 0 0 159 3944521 8709 0.141834 88694253 ## HISTOGRAM java.lang.Double BIN CoverageMult all_sets optical_sets non_optical_sets 1.0 1.000267 21231956 0 21238466 2.0 1.7313 2176139 8697 2171200 3.0 2.265566 288651 6 287379 4.0 2.656028 73318 0 73101 5.0 2.941393 32190 0 32151 6.0 3.149948 17562 0 17542 7.0 3.302368 11054 0 11046 8.0 3.413763 7372 0 7371 9.0 3.495174 5201 0 5201 10.0 3.554673 3708 0 3703 11.0 3.598156 2794 0 2792 12.0 3.629936 2218 0 2218 13.0 3.653162 1688 0 1685 14.0 3.670136 1413 0 1418 15.0 3.682541 1084 0 1080 16.0 3.691608 959 0 955 17.0 3.698234 780 0 783 18.0 3.703076 721 0 717 19.0 3.706615 595 0 595 20.0 3.709202 502 0 502 21.0 3.711092 481 0 483 22.0 3.712474 411 0 410 23.0 3.713483 378 0 378 24.0 3.714221 317 0 320 25.0 3.714761 291 0 287 26.0 3.715155 284 0 285 27.0 3.715443 240 0 241 28.0 3.715653 195 0 194 29.0 3.715807 204 0 205 30.0 3.71592 172 0 170 31.0 3.716002 186 0 186 32.0 3.716062 148 0 149 33.0 3.716106 156 0 155 34.0 3.716138 111 0 112 35.0 3.716161 128 0 128 36.0 3.716178 118 0 119 37.0 3.716191 112 0 110 38.0 3.7162 115 0 115 39.0 3.716207 82 0 82 40.0 3.716212 103 0 105 41.0 3.716215 70 0 70 42.0 3.716218 89 0 87 43.0 3.71622 90 0 92 44.0 3.716221 68 0 67 45.0 3.716222 69 0 68 46.0 3.716223 67 0 67 47.0 3.716223 62 0 62 48.0 3.716224 50 0 54 49.0 3.716224 54 0 50 50.0 3.716224 53 0 53 51.0 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