FastQCFastQC Report
Wed 26 Feb 2014
B188i_DC_24h_RNA_Seq.bam

Summary

[OK] Basic Statistics

Measure Value
Filename B188i_DC_24h_RNA_Seq.bam
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 21748875
Filtered Sequences 0
Sequence length 42
%GC 53

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 3068830 9.183714 14.185614 10
TTCAC 5294975 5.765454 30.389143 24
TTTTT 5107380 5.7333922 7.218553 6
CAGGC 4688845 5.319 15.712261 29
GGAGT 3554280 5.162064 20.680077 10
TGGAG 3432165 4.98471 20.857742 9
CACAG 3310075 4.475804 18.845892 27
ATTCA 2967130 4.4022803 20.284332 24
ACAGG 2810650 4.344527 21.109741 28
TCACA 3111665 4.122197 18.138933 26
CTGGA 3242650 4.1197357 17.417387 8
GCTAT 3273390 4.0744567 16.368237 21
AGTGG 2788870 4.0504193 19.548454 17
CAGTG 3169690 4.0270414 17.521917 16
TGCAG 3128230 3.9743671 17.596008 14
CCTCC 5386680 3.8433845 15.164666 13
GAGTG 2610505 3.791371 19.247719 11
GTTCA 2895395 3.6039586 25.227865 23
GCAGT 2818525 3.5808914 17.158712 15
AAAAT 1429920 3.51715 6.134637 10
GTGCA 2751240 3.4954073 17.17292 13
AGTGC 2685905 3.4123998 17.117413 12
TATTC 2736510 3.3371193 16.402723 23
GGCTA 2605740 3.3105514 16.687216 20
CTATT 2711575 3.3067114 16.215422 22
GGGAG 2218755 3.28911 7.13081 19
TGGCT 3142975 3.2820396 14.277524 19
CCGCC 4485250 3.2664547 14.998243 10
GCTGG 2953715 3.1482465 14.594326 7
AGGCT 2465955 3.1329572 15.785031 5
GTGGC 2933305 3.1264925 14.380585 18
CGGGG 2865390 3.1173182 21.042175 32
TAAAA 1267115 3.1167014 7.0569353 9
AGGCG 2376240 3.0814626 17.331968 30
CCGGG 3232300 3.0761435 19.33396 31
GGCTG 2848005 3.0355747 14.3238945 6
GCGCG 3167720 3.0146835 13.136675 32
GCCGG 3119690 2.9689739 18.232857 30
CTCCT 3680920 2.9414015 15.764778 14
GGGGC 2698315 2.935554 20.81173 33
CAGCA 2058965 2.784083 7.394804 34
TCACC 2863045 2.783508 19.812931 25
ACAAA 1251445 2.7484367 6.766237 38
AAATA 1101765 2.7099926 5.1653895 6
GGCGG 2461205 2.677597 20.530416 35
GCGAT 2100055 2.6680865 16.543152 34
CCGTT 2905820 2.6544163 18.126154 21
GGGCG 2424480 2.6376429 20.444624 34
CTCCC 3645315 2.600925 15.497216 17
TCCTC 3239735 2.5888531 15.862229 15
GGCGC 2718200 2.586881 12.794042 31
TCCCA 2556585 2.4855618 13.462973 38
CGTTC 2703010 2.469153 17.599354 22
CCAGG 2170565 2.4622767 10.748262 3
CGCGA 2160155 2.4504678 14.812092 33
GGTTC 2334745 2.4380484 20.855644 5
ACCGC 2438770 2.4200954 18.669994 27
GATCC 2170410 2.4121737 14.885814 36
ACTGA 1582485 2.3965032 10.039609 38
CCCCG 3238610 2.3585691 14.191307 19
CGATC 2108285 2.3431284 14.699603 35
TTAAA 1129320 2.2831254 5.4274035 8
CACCG 2291215 2.2736702 18.532314 26
AATAA 912440 2.2443132 5.3641863 7
GCCTC 2747010 2.2405539 15.957187 12
TTCTT 2212655 2.2177982 6.0915594 6
ATCAG 1463020 2.2155862 7.2359033 32
CGCCG 2640590 2.198333 15.984515 29
GCGGC 2295305 2.1844158 17.75923 36
GGAGG 1467510 2.175455 5.212765 16
ATCCC 2229165 2.1672375 13.065051 37
AGCAC 1589495 2.149277 9.387582 10
GGAAG 1211580 2.1408699 11.303784 5
AGTTT 1520420 2.1195357 5.1354585 22
GAAGA 996400 2.098655 13.793174 6
TCCCC 2939355 2.097224 13.816744 18
GGCTC 2237000 2.0857568 17.539215 38
ATGTT 1495520 2.084824 7.4613414 7
AGCAG 1335520 2.0643632 5.505905 25
ACTAC 1552770 2.0570414 8.3961735 35
TTTGA 1472525 2.052768 5.0566745 25
CTCAG 1839985 2.0449426 9.139693 1
CCCGT 2492420 2.0329018 15.484214 20
GATCA 1341940 2.0322237 7.0992274 31
TCAGG 1588060 2.0176053 8.243307 2
CCCAG 1976845 1.9617072 8.138399 2
CCACT 2017120 1.9610834 6.5692506 33
CCCGC 2688920 1.9582487 13.99268 9
TTTAA 1150750 1.9121734 5.05686 7
CACTA 1442770 1.9113183 8.381316 34
TCTTC 2133760 1.9096276 5.46889 7
CCCAC 2192770 1.9034971 6.8891864 38
TCAGC 1701205 1.8907036 5.641878 33
CTACT 1720050 1.8728826 7.1004424 36
TGATC 1490835 1.8556737 7.9056416 38
TGTTG 1576310 1.8435279 6.0976686 8
CTGAT 1473285 1.8338286 5.849301 29
CGGCT 1966680 1.8337129 17.074913 37
TACTG 1469390 1.8289804 8.043805 37
GGGGA 1200800 1.7800809 5.1121564 29
AAGAG 831340 1.7509992 13.414451 7
ACGGG 1338430 1.7356502 6.086065 38
CGGTT 1647380 1.7202698 19.76542 4
CGCCT 2108735 1.7199554 15.294391 11
TTCCC 2133800 1.7051071 15.534941 7
TCCCG 2085570 1.7010612 15.511604 8
GTTCC 1851705 1.6915008 17.562088 6
TTGGA 1177950 1.6761054 5.056742 14
TATGT 1165270 1.6244402 6.937355 6
CTTGA 1293290 1.6097851 5.1649055 1
ACACA 998080 1.6086729 9.517011 32
GAGAG 890270 1.573113 6.059723 4
TGAGA 907380 1.5708333 5.82799 3
TCCAG 1367595 1.5199326 6.815108 25
CTGAA 1001160 1.516149 8.858012 19
CTCCA 1504470 1.4626751 6.700041 1
GCCCA 1464995 1.453777 7.236759 1
AGAGC 934815 1.4449785 10.473307 8
GTTGC 1374790 1.4356191 5.1104736 9
CTCGT 1566305 1.4307928 10.074356 32
TCGGT 1369585 1.4301838 19.732098 3
TTCGG 1366135 1.4265811 19.816006 2
GCTCA 1283120 1.4260478 6.624165 1
CACGG 1218475 1.3822311 5.178525 37
CTATG 1056870 1.3155081 6.071297 5
GCTCG 1407405 1.3122505 9.627161 31
GCACG 1143425 1.2970948 5.029371 36
GTCCG 1363850 1.2716404 9.479761 35
CGTCC 1558730 1.2713528 8.543827 34
CCGCT 1549415 1.2637551 8.5666065 37
TCGTC 1362020 1.2441818 9.292995 33
AGTCA 808965 1.2250904 8.547879 28
TCTGA 981755 1.222011 7.222946 18
CACAC 1019530 1.205954 6.8517957 12
ACAGT 789830 1.1961125 8.451378 34
CTTCG 1253845 1.145366 17.303497 1
AGTGA 659845 1.1423069 9.440523 36
GAGCA 687995 1.0634596 9.179632 9
TCCGC 1277430 1.0419152 8.335289 36
GCACA 728995 0.98572946 7.766093 11
CCAGT 879855 0.9778627 6.276121 26
GAACT 633185 0.9588905 8.352216 21
TGAAC 629785 0.9537416 8.423063 20
CGCTC 1155275 0.94228125 8.47093 38
GTCTG 896030 0.9356758 5.9807467 17
CAGTC 742315 0.8250021 6.2569013 27
GTGAT 578230 0.8227636 7.659023 37
AACTC 614585 0.81417525 7.3461213 22
ACTCC 820360 0.79756993 5.559143 23
GTCAC 673845 0.7489052 6.1694546 29
CGCTA 664520 0.7385414 5.2157507 3
ACACG 520175 0.7033682 7.2390385 13
CGGAA 338900 0.5238503 8.416325 4
CACGT 461195 0.5125679 5.9712987 14
CGTCT 512120 0.4678128 5.0020223 16
TCGGA 342825 0.43555376 7.0049763 3
ACGTC 336805 0.37432197 5.847376 15
ATCGG 279670 0.35531634 6.6053133 2
GATCG 256240 0.32554892 6.578536 1
Produced by FastQC (version 0.10.1)