FastQC Report![[OK]](Icons/tick.png)
Basic Statistics
| Measure | Value |
|---|---|
| Filename | B188i_DC_1h_RNA_Seq.bam |
| File type | Conventional base calls |
| Encoding | Sanger / Illumina 1.9 |
| Total Sequences | 18436901 |
| Filtered Sequences | 0 |
| Sequence length | 42 |
| %GC | 51 |
Per base sequence quality
Per sequence quality scores
![[FAIL]](Icons/error.png)
Per base sequence content
Per base GC content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
No overrepresented sequences
Kmer Content
| Sequence | Count | Obs/Exp Overall | Obs/Exp Max | Max Obs/Exp Position |
|---|---|---|---|---|
| AAAAA | 3048520 | 7.330757 | 11.9118185 | 10 |
| TTTTT | 4445860 | 5.2065043 | 6.8640656 | 6 |
| CAGGC | 2695435 | 3.9778652 | 10.661101 | 29 |
| TTCAC | 3094660 | 3.8706844 | 18.184126 | 24 |
| TGGAG | 2096335 | 3.633411 | 12.698685 | 9 |
| GGAGT | 2040915 | 3.5373554 | 12.85408 | 10 |
| CCTCC | 3249110 | 3.2548866 | 10.913118 | 13 |
| CACAG | 2070390 | 3.2482095 | 11.589856 | 27 |
| CTGGA | 2100585 | 3.2234144 | 10.800195 | 8 |
| GGGAG | 1666785 | 3.1380265 | 6.0732536 | 19 |
| AAAAT | 1495180 | 3.1135755 | 5.4859905 | 10 |
| CCGGG | 2202670 | 3.0577354 | 14.760403 | 31 |
| ACAGG | 1725345 | 3.057354 | 12.625402 | 28 |
| TGCAG | 1986415 | 3.048217 | 11.095484 | 14 |
| CGGGG | 1837425 | 2.8809655 | 15.210372 | 32 |
| CAGTG | 1865470 | 2.862623 | 10.925546 | 16 |
| GCCGG | 2049655 | 2.845321 | 13.188517 | 30 |
| GCTGG | 1954615 | 2.8214118 | 10.202281 | 7 |
| TAAAA | 1345365 | 2.801599 | 6.4752803 | 9 |
| GGGGC | 1784185 | 2.7974887 | 15.065593 | 33 |
| AGTGG | 1613200 | 2.7960312 | 11.825062 | 17 |
| GGCTG | 1891320 | 2.7300475 | 10.319045 | 6 |
| CTCCT | 2596695 | 2.7048745 | 10.496977 | 14 |
| TCACA | 1862085 | 2.689479 | 10.146539 | 26 |
| ATTCA | 1783130 | 2.6779742 | 10.539224 | 24 |
| CCGCC | 2443705 | 2.6591537 | 11.6406765 | 10 |
| GTTCA | 1865270 | 2.6350837 | 14.926168 | 23 |
| GAGTG | 1487680 | 2.5784776 | 11.521033 | 11 |
| CTCCC | 2572450 | 2.5770235 | 12.154789 | 17 |
| TGGCT | 1921235 | 2.5530689 | 9.353332 | 19 |
| GCAGT | 1648005 | 2.5289161 | 10.553608 | 15 |
| CCAGG | 1686525 | 2.4889374 | 9.137155 | 3 |
| GTGGC | 1714145 | 2.4743025 | 9.968654 | 18 |
| CAGCA | 1571260 | 2.4651306 | 5.0831614 | 34 |
| GTGCA | 1582340 | 2.4281514 | 10.440499 | 13 |
| GCTAT | 1718430 | 2.4276416 | 9.237602 | 21 |
| GGCGG | 1548125 | 2.4273615 | 14.620434 | 35 |
| ACAAA | 1199130 | 2.4014711 | 5.20168 | 38 |
| AGGCT | 1562455 | 2.397637 | 9.771726 | 5 |
| AGTGC | 1532505 | 2.3516777 | 10.303208 | 12 |
| GCGCG | 1672945 | 2.3223739 | 9.612607 | 32 |
| GGGCG | 1455990 | 2.2828996 | 14.523915 | 34 |
| TCCTC | 2159085 | 2.2490335 | 10.57375 | 15 |
| TCACC | 1851790 | 2.2274697 | 12.83217 | 25 |
| AGGCG | 1280375 | 2.1342056 | 11.224152 | 30 |
| GGCTA | 1387680 | 2.129439 | 9.963277 | 20 |
| CTCAG | 1515805 | 2.059403 | 9.2180605 | 1 |
| TATTC | 1582945 | 2.058711 | 8.896857 | 23 |
| CCCCG | 1871890 | 2.0369246 | 10.489827 | 19 |
| CCCAG | 1549525 | 2.0246122 | 6.8804617 | 2 |
| CCGTT | 1714115 | 2.016713 | 11.5485115 | 21 |
| CTATT | 1543335 | 2.0071957 | 8.707026 | 22 |
| TCCCA | 1660305 | 1.9971373 | 8.6377535 | 38 |
| GGCGC | 1414340 | 1.9633799 | 9.270608 | 31 |
| GCGGC | 1408415 | 1.9551548 | 12.702859 | 36 |
| GAAGA | 908490 | 1.9330298 | 5.0212326 | 6 |
| TCCCC | 1928945 | 1.9323745 | 9.716725 | 18 |
| CGCCG | 1567650 | 1.9267337 | 11.632747 | 29 |
| GCCTC | 1702615 | 1.9264861 | 11.312101 | 12 |
| GGTTC | 1447905 | 1.9240754 | 13.491071 | 5 |
| CGTTC | 1609530 | 1.8936651 | 11.090906 | 22 |
| GGCTC | 1474190 | 1.8839995 | 11.97359 | 38 |
| ACCGC | 1432630 | 1.8718768 | 11.930124 | 27 |
| TCAGG | 1217845 | 1.8688219 | 6.950279 | 2 |
| TTTAA | 1190570 | 1.85923 | 5.054955 | 7 |
| ACTGA | 1079705 | 1.7613745 | 5.3764606 | 38 |
| GGAGA | 856155 | 1.7135632 | 5.7262874 | 38 |
| TCCAG | 1242585 | 1.6882007 | 7.1678057 | 2 |
| CACCG | 1290290 | 1.6858952 | 11.841934 | 26 |
| CCCGC | 1524885 | 1.6593261 | 10.272946 | 9 |
| CCCAC | 1394950 | 1.6137044 | 5.052042 | 38 |
| CTCCA | 1339155 | 1.6108345 | 8.647715 | 1 |
| ATGTT | 1078495 | 1.5842569 | 5.0730515 | 7 |
| CCCGT | 1399785 | 1.5838377 | 10.513297 | 20 |
| GATCC | 1157945 | 1.5732071 | 9.063957 | 36 |
| GCGAT | 1024020 | 1.5713912 | 9.814855 | 34 |
| TCAGA | 931975 | 1.5203755 | 5.62921 | 3 |
| CGCGA | 1014350 | 1.496956 | 9.415829 | 33 |
| ATCCC | 1239630 | 1.4911184 | 8.106275 | 37 |
| CGGCT | 1161855 | 1.4848386 | 11.414768 | 37 |
| CTCTC | 1412435 | 1.4712777 | 5.068086 | 1 |
| TTCCC | 1377205 | 1.4345801 | 10.144371 | 7 |
| CGATC | 1029875 | 1.3992089 | 8.93827 | 35 |
| GCCCA | 1049765 | 1.3716251 | 5.6957464 | 1 |
| TCCCG | 1203795 | 1.3620778 | 10.548676 | 8 |
| GTTCC | 1142035 | 1.3436419 | 11.232904 | 6 |
| TCTGG | 1005100 | 1.3356457 | 5.6656237 | 2 |
| CGGTT | 971185 | 1.2905772 | 12.318011 | 4 |
| CGCCT | 1125205 | 1.2731544 | 10.346644 | 11 |
| TCCTG | 1078840 | 1.2692908 | 6.296997 | 2 |
| CTCTG | 1066590 | 1.2548783 | 6.736094 | 1 |
| CTCGT | 1058475 | 1.2453307 | 6.4214134 | 32 |
| GTCCG | 934575 | 1.1943773 | 6.266588 | 35 |
| CGTCC | 1051560 | 1.189826 | 5.7790527 | 34 |
| CCGCT | 1037220 | 1.1736004 | 5.771181 | 37 |
| CTCTT | 1046065 | 1.1330253 | 5.6518307 | 1 |
| GCTCG | 880055 | 1.1247013 | 6.36681 | 31 |
| TCGTC | 888025 | 1.0447906 | 5.7798877 | 33 |
| CAGAC | 663210 | 1.040502 | 5.0238605 | 4 |
| TTCGG | 747755 | 0.99366814 | 12.543436 | 2 |
| TCCGC | 870715 | 0.98520225 | 5.5490737 | 36 |
| CGCTC | 865995 | 0.97986174 | 5.7466373 | 38 |
| TCGGT | 733080 | 0.9741669 | 12.344011 | 3 |
| CTTCG | 702440 | 0.82644385 | 11.218105 | 1 |