FastQCFastQC Report
Thu 6 Jul 2023
D6_R2_VP2_S2_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameD6_R2_VP2_S2_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1104128
Total Bases65.1 Mbp
Sequences flagged as poor quality0
Sequence length59
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGTGCTGGCCACGTGTGATCTTAGAATCTTGGTGTCTGCCTTCTCGTGG18831717.05572180037097No Hit
AAGTGCTGGCCACGTCTGGTCAAGAGAGGGCTTCGGAAGAGCACACGTCT803377.276058572919082No Hit
AAGTGCTGGCCATGTCTGGTCAAGAGAGGGCTTCGGAAGAGCACACGTCT429143.8866870507767217No Hit
AAGTGCTGGCCACGTGTGGTCAAGAGAGGGCTTCGGAAGAGCACACGTCT369623.347619116624159No Hit
AAGTGCTGGCCACGTGTGATCAAGAGAGGGCTTCGGAAGAGCACACGTCT280262.53829266172038No Hit
AAGTGCTGGCCAAGAGAGGGCTTCGGAAGAGCACACGTCTGAACTCCAGT121631.1015932935311847Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
AAGTGCTGGTCAAGAGAGGGCTTCGGAAGAGCACACGTCTGAACTCCAGT79260.717851553443079Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
AAGTGCTGGCCACGTGTGATCTGGTCAAGAGAGGGCTTCGGAAGAGCACA55680.5042893577556226No Hit
AAGTGCTGGCCACGTGTGATCTCCAGTCATGTCTGGTCAAGAGAGGGCTT38250.3464272258288894No Hit
AAGTGCTGGCCACGTGTGTCTGGTCAAGAGAGGGCTTCGGAAGAGCACAC37460.33927225828889407No Hit
AAGTGCTGGCCACGTGTGATCTCCAGCATGTCTGGTCAAGAGAGGGCTTC37270.33755144331092046No Hit
AAGTGCTGGCCACGTCTGGTCAAGAGAGGGCTTCGGAAAAGCACACGTCT32800.2970670067238581No Hit
AAGTGCTGGCCACGTGTGATCAGCTACTTAAGAGCCCTGACTTTATAGGT27200.24634824947832135No Hit
AAGTGCTGGCCACGTGTGATCTTAGAATCTTGGTGTCTGCCGTCTCGTGG25830.23394026779503824No Hit
AAGTGCTGGCCACGTGTGGTCTGGTCAAGAGAGGGCTTCGGAAGAGCACA23490.21274707280315325No Hit
AAGTGCTGGCCACGTGTGATGTCTGGTCAAGAGAGGGCTTCGGAAGAGCA23460.21247536517505217No Hit
AAGTGCTGGCCACGTGTGATCATGTCTGGTCAAGAGAGGGCTAAGGAACT23400.21193194991884998No Hit
AAGTGCTGGCCACGTGTCTGGTCAAGAGAGGGCTTCGGAAGAGCACACGT22910.20749405865986553No Hit
AAGTGCTGGCCACGAGAGGGCTTCGGAAGAGCACACGTCTGAACTCCAGT20680.18729712497101786Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
AAGTGCTGGCCACGTCTGGTCAAGAGAGGGCTTCGGAAGAGCACACGACT19200.17389288198469743No Hit
TAGTGCTGGCCACGTGTGATCTTAGAATCTTGGTGTCTGCCTTCTCGTGG17660.15994522374217482No Hit
AAGTGCTGGCCATGTCTGGTCAAGAGAGGGCTTCGGAAAAGCACACGTCT17510.15858668560166939No Hit
AAGTGCTGGCCACGTCTGGTCAAGAGAGGGCTTCGGAAGAGCACACCTCT16780.15197513331787618No Hit
AAGTGCTGGCCACGTGTGATCTCCAGTATGTCTGGTCAAGAGAGGGCTTC16490.14934862624623232No Hit
AAGTGCTGGCCACGTGTGGTCAAGAGAGGGCTTCGGAAAAGCACACGTCT15480.1402011361001623No Hit
AAGTGCTGGCCACGTGTGATCATACTCTTTGCCTACCATCTATTTTATCC14420.13060079990725712No Hit
AAGTGCTGGCCATGTGTGGTCAAGAGAGGGCTTCGGAAGAGCACACGTCT12270.11112841989334571No Hit
AAGTGCTGGCCACGTGTGATCAAGAAACTTCAAGGAGGCAGGGTACAATG12120.10976988175284025No Hit
AAGTGCTGGCCACGTGTGATCAAGAGAGGGCTTCGGAAAAGCACACGTCT12110.10967931254347323No Hit
AAGTGCTGGCCACGTATGTCTGGTCAAGAGAGGGCTTCGGAAGAGCACAC11480.10397345235335033No Hit
AAGTGCTGGCCACGTGTGATCAAGAGAGTAGGGCTGGAATGGCTGCTTTA11410.10333946788778113No Hit
AAGTGCTGGCCACGTGTGATCTCAGGTCACTGCAACCTCCGCCTCCTGGG11380.10306776025968004No Hit
AAGTGCTGGCCACATGTCTGGTCAAGAGAGGGCTTCGGAAGAGCACACGT11100.10053182239740321No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)