FastQCFastQC Report
Thu 6 Jul 2023
D3_R2_VP6_S6_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameD3_R2_VP6_S6_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1866494
Total Bases110.1 Mbp
Sequences flagged as poor quality0
Sequence length59
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTGCCAGTAGGGAAGGATCCCTGTTATAACCCCTCTCCAGGCAGGCTT92331449.46782577388408No Hit
ACCTGCCAGTAGGGAAGGATCACAGGTGAGTCAAACAGGAAGCCTGCTCC303071.623739481616335No Hit
ACCTGCCAGTAGGGAAGGATCCCTGTTATAACCCCTCTCCAGGCAGGCGT107660.5768033543102737No Hit
ACCTGCCAGTAGGGAAGGATCCCTGTTATAACCCCACTCCAGGCAGGCTT67610.3622299348404013No Hit
ACCTGCCAGTAGGGAAGGATCCCTGTTATAACCCCTCTCCAGGCCGGCGT50120.26852483854756565No Hit
ACCTGCCAGTAGGGAAGGATCCCTGTTATAACCCCTCACCAGGCAGGCTT41820.2240564395063686No Hit
ACCTGCCAGTAGGGAAGGATCCCTGTTATAACCCCTCTCCAGGCGGGCTT35670.19110696310837325No Hit
ACCTGCCAGTAGGGAAGGATCCCTGTTATAACCCCTCTCCAGGCCGGCTT33060.17712352678337032No Hit
ACCTGCCAGTAGGGAAGGATCCCTGTTATAACCCCCCTCCAGGCAGGCTT28500.15269269550290543No Hit
ACCTGCCAGTAGGGAAGGATCCCTGCTATAACCCCTCTCCAGGCAGGCTT27670.14824585559878575No Hit
ACCTGCCAGTAGGGAAGGATCCCTGTTATAACCCCTCCCCAGGCAGGCTT27530.14749578621736797No Hit
ACCTGCCAGTAGGGAAGGATCATGAGGAGACCAGGAGGACATTCCACAGT25660.13747700233700189No Hit
ACCTGCCAGTAGGGAAGGATCCCTGTTATAACCCCTCTCCGGGCAGGCTT25600.13715554403068No Hit
ACCTGCCAGTAGGGAAGGACCCCTGTTATAACCCCTCTCCAGGCAGGCTT25160.13479818311765268No Hit
ACCTGCCAGTAGGGAAGGATCCCTGTTATAACCCCTCTCCACGCAGGCTT24700.1323336694358514No Hit
ACCTGCCAGTAGGGAAGGATCCAGATTCCTGGGAATCCTATAAGCCCTCC23730.1271367601503139No Hit
ACCTGCCAGTAGGGAAGGATCCCCGTTATAACCCCTCTCCAGGCAGGCTT23530.12606523246257423No Hit
ACCTGCCAGTAGGGAAGGATCCCTGTTATAGCCCCTCTCCAGGCAGGCTT22850.12242203832425928No Hit
ACCTGCCAGTAGGGAAGGATCCCTGTTATAACCCCTCTCCAGGCAGGCCT22020.1179751984201396No Hit
ACCTGCCAGTAGGGAAGGATCCCTGTTTTAACCCCTCTCCAGGCAGGCTT21770.11663578881046498No Hit
ACCTGCCAGTAGGGAAGGATCCCTGTTATAACCCCTCTCCAGGCAGGCTA21610.11577856666027321No Hit
ACCTGCCAGTAGGGAAGGATCCCTGTTATAACCCCTCTCCACGCCGGCGT20660.1106888101435097No Hit
ACCTGCCAGTAGGGAAGGATCCCTGTTGTAACCCCTCTCCAGGCAGGCTT20570.11020662268402684No Hit
ACCTGCCAGTAGGGAGGGATCCCTGTTATAACCCCTCTCCAGGCAGGCTT19800.10608124108622906No Hit
ACCTGCCAGTAGGGAAGGATCCCTGTTATAACCCCTCTCCAGGCAGGCTC19740.10575978277990715No Hit
ACCTGCCAGTAGGGAAGGATCCCTGTTATAACCCCACACCAGGCAGGCTT19220.10297381079178394No Hit
ACCTGCCAGTAGGGAAGGATCCCTGTTACAACCCCTCTCCAGGCAGGCTT19080.10222374141036618No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)