FastQCFastQC Report
Thu 6 Jul 2023
D3_R2_VP3_S3_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameD3_R2_VP3_S3_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1207695
Total Bases71.2 Mbp
Sequences flagged as poor quality0
Sequence length59
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTCCTGTCACTGGCTTTGATCCTCCTTCGTTCAGCTTGTAATCACATC52109943.14822865044569No Hit
CCTTCCTGTCACTGGCTTTGATCTGATACATACGTTGCACAACCTATTTG75990.6292151578006037No Hit
CCTTCCTGTCACTGGCTTTGATCCTCCTTCGTTCAGCTTGTAATCACAAC69090.5720815272067864No Hit
CCTTCCTGTCACTGGCTTTGATCCTCCTTCGTTCAGCTTGTAATAACATC56620.4688269803220184No Hit
CCTTCCTGTCACTGGCTTTGATCATGTCTTCATCTCTGTGGTCCCACCAT26100.21611416789835183No Hit
CCTTCCTGTCACTGGCTTTGATCCTCCTTCGTTCAGCTTGTAATCACATA24200.20038171889425724No Hit
CCTTCCTGTCACTGGCTTTGATCCTGTGGAGGCAAAACAGAGGAGTCCCC23850.19748363618297665No Hit
CCTTCCTGTCACTGGCTTTGATCCAAAGGAAGGACAAGGTCTTATTGGTC23150.1916874707604155No Hit
CCTTCCTGTCACTGGCTTTGATCGGAAGGAAGTGCTGTGGCCTGAGCGGT21650.17926711628349873No Hit
CCTTCCTGTCACTGGCTTTGATCCTCCTTCGTTCAGCTTGTAATAACAAC21420.1773626619303715No Hit
CCTTCCTGTCACTGGCTTTGATCCTCCTTCGCTCAGCTTGTAATCACATC20070.1661843429011464No Hit
CCTTCCTGTCACTGGCTTTGATCCTCCTTCGTTCAGCCTGTAATCACATC19350.16022257275222634No Hit
CCTTCCTGTCACTGGCTTTGATCAACCTTGATAACTGGCTGCGAAGCCCA18000.14904425372300126No Hit
CCTTCCTGTCACTGGCTTTGATCCTCCCTCGTTCAGCTTGTAATCACATC17670.14631177573807955No Hit
CCTTCCTGTCACTGGCTTTGATCACAGCAGCCTCAAACCCTTCCTCAGAG16520.13678950397244335No Hit
CCTTCCTGTCACTGGCTTTGATCCACCCGCCTCGGCCTCCCAAAGTGCTG15960.13215257163439445No Hit
CCTTCCTGTCACTGGCTTTGATCACTAAAAACCCTGATTTGGAAAGTCCC15460.1280124534754222No Hit
CCTTCCTGTCACTGGCTTTGATCCTCCTTCGTTCGGCTTGTAATCACATC14950.12378953295327048No Hit
CCTTCCTGTCACTGGCTTTGATCCACCCACCTTGGCCTCCCAAAGTGCTG14670.12147106678424603No Hit
CCTTCCTGTCACTGGCTTTGATCCTCCTTCGTTCAGCTTGTAGTCACATC14500.12006342661019546No Hit
CCTTCCTGTCACTGGCTTTGATCCCCCTTCGTTCAGCTTGTAATCACATC14360.11890419352568324No Hit
CCTTCCTGTCACTGGCTTTGATCCTCCTTCGTTCAGCTTGTAATCACGTC14350.11882139116250379No Hit
CCCTCCTGTCACTGGCTTTGATCCTCCTTCGTTCAGCTTGTAATCACATC14130.11699973917255599No Hit
CCTTCCTGTCACTGGCTTTGATCCTCCTTCGTCCAGCTTGTAATCACATC13690.1133564351926604No Hit
CCTTCCTGTCACTGGCTTTGATCCTCCTTCGTTCAGCTTGTAATAACATA13580.1124456091976865No Hit
CCTTCCTGTCACTGGCTTTGATCCGCCCGCCTCGGCCTCCCAAAGTGCTG13510.11186599265543039No Hit
CCTTCCTGTCACTGGCTTTGATCCTCCTTCGTTCAGCTTGTAATCACACC13500.11178319029225095No Hit
CCTTCCTGTCACTGGCTTTGATCCTCCTTCGTTCAGCTTGTAACCACATC13420.11112077138681538No Hit
CCTTCCTGTCACTGGCTTTGATCCTCCTTCGTTCAGCTCGTAATCACATC12690.10507619887471589No Hit
CCTTCCTGTCACTGGCTTTGATCCAAGAGGGAGAAGAAGCCCATTGGAGA12660.10482779178517755No Hit
CCTTCCTGTCACTGGCTTTGATCCTCCTTCGTTCAGCTTGTAATCGCATC12410.10275773270569141No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)