FastQCFastQC Report
Thu 6 Jul 2023
D3_R2_VP2_S1_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameD3_R2_VP2_S1_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences812272
Total Bases47.9 Mbp
Sequences flagged as poor quality0
Sequence length59
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGTGCTGGCCACGTGTGATCTTAGAATCTTGGTGTCTGCCTTCTCGTGG15163418.667884649477024No Hit
AAGTGCTGGCCACGTGTGATCAGCTACTTAAGAGCCCTGACTTTATAGGT46840.5766541257115844No Hit
AAGTGCTGGCCACGTGTGATCCAGGGAGAGATCTGGACTGAAATGTACAC23180.28537238757460553No Hit
AAGTGCTGGCCACGTGTGATCTTAGAATCTTGGTGTCTGCCGTCTCGTGG21920.2698603423483759No Hit
AAGTGCTGGCCACGTGTGATCATGTCTGGTCAAGAGAGGGCTAAGGAACT21450.26407410325605213No Hit
AAGTGCTGGCCACGTGTGATCATACTCTTTGCCTACCATCTATTTTATCC17930.2207388657986487No Hit
AAGTGCTGGCCACGTGTGATCAGTAGAAGTACTTTCAACAGCAATGTCTG15000.18466720507416234No Hit
AAGTGCTGGCCACGTGTGATCCTCCACCTCAGCCTCCTGAGTAGCTAGGA14070.17321783835956428No Hit
TAGTGCTGGCCACGTGTGATCTTAGAATCTTGGTGTCTGCCTTCTCGTGG13000.16004491106427404No Hit
AAGTGCTGGCCACGTGTGATCCAGACTCATTCCTGCCTTAAGGTCTTGCA12540.1543817834419997No Hit
AAGTGCTGGCCACGTGTGATCATAGCACCCTGAATTAAGTTGTTTACTTA11700.14404041995784664No Hit
AAGTGCTGGCCACGTGTGATCAGCCCTCTCCCTCTACAAACATACATACT11560.14231685937715446No Hit
AAGTGCTGGCCACGTGTGATCAAGAGAGTAGGGCTGGAATGGCTGCTTTA10900.13419150235389132No Hit
AAGTGCTGGCCACGTGTGATCTCAGGTCACTGCAACCTCCGCCTCCTGGG10880.13394527941379245No Hit
AAGTGCTGGCCACGTGTGATCACCTGAGGTCAGGAGTTTGAGACCAGCCT10720.13197549589300137No Hit
AAGTGCTGGCCACGTGTGATCAAGTCTGAAAAACAAACCACAGAAAGAAT9750.12003368329820553No Hit
AAGTGCTGGCCACGTGTGATCTAAGGTTTCAATTAAAAAGCTAGGAAAAG9740.11991057182815608No Hit
AAGTGCTGGCCACGTGTGATCCTTGCCTAGAGCAAAACTCATTCAGTCCT9630.11855634565761224No Hit
AAGTGCTGGCCACGTGTGATCAAGAAACTTCAAGGAGGCAGGGTACAATG8840.10883053952370635No Hit
AAGTGCTGGCCACGTGTGATCTTTTAAAAAGATTGAGAAAAAATCCCTTT8610.1059989757125692No Hit
AAGTGCTGGCCACGTGTGATCCACGCACCTTGGCCTCCCAAAGTGCTGGG8280.10193629720093762No Hit
AAGTGCTGGCCACGTGTGATCACTGGAGGTCAGGACTTCAAGACCAACCT8250.10156696279078929No Hit
AAGTGCTGGCCACGTGTGATCTTGGCTCACTGCAACCTCTGCCTCCCAGG8240.10144385132073985No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)