FastQCFastQC Report
Thu 6 Jul 2023
D3_R1_VP6_S5_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameD3_R1_VP6_S5_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1327853
Total Bases78.3 Mbp
Sequences flagged as poor quality0
Sequence length59
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTGCCAGTAGGGAAGGATCCCTGTTATAACCCCTCTCCAGGCAGGCTT67621350.92529067600103No Hit
ACCTGCCAGTAGGGAAGGATCACAGGTGAGTCAAACAGGAAGCCTGCTCC164891.2417790222261047No Hit
ACCTGCCAGTAGGGAAGGATCCCTGTTATAACCCCTCTCCAGGCAGGCGT80430.605714638593278No Hit
ACCTGCCAGTAGGGAAGGATCCCTGTTATAACCCCACTCCAGGCAGGCTT49000.3690167511012138No Hit
ACCTGCCAGTAGGGAAGGATCCCTGTTATAACCCCTCTCCAGGCGGGCTT38210.2877577563179057No Hit
ACCTGCCAGTAGGGAAGGATCCCTGTTATAACCCCTCTCCAGGCCGGCGT36910.27796751598256736No Hit
ACCTGCCAGTAGGGAAGGATCCCTGTTATAACCCCTCCCCAGGCAGGCTT33540.25258820065172877No Hit
ACCTGCCAGTAGGGAAGGATCCCTGTTATAACCCCCCTCCAGGCAGGCTT31980.24083991224932277No Hit
ACCTGCCAGTAGGGAAGGATCCCTGCTATAACCCCTCTCCAGGCAGGCTT30460.22939286201108106No Hit
ACCTGCCAGTAGGGAAGGATCCCTGTTATAACCCCTCACCAGGCAGGCTT30020.22607924220527423No Hit
ACCTGCCAGTAGGGAAGGATCCCTGTTATAACCCCTCTCCGGGCAGGCTT29450.22178659836593356No Hit
ACCTGCCAGTAGGGAAGGATCCCTGTTATAGCCCCTCTCCAGGCAGGCTT26110.19663321165821818No Hit
ACCTGCCAGTAGGGAAGGATCTCATTGAATTTCTTTAAAGGTGGAATCTC25200.18978004342348137No Hit
ACCTGCCAGTAGGGAAGGATCCCTGTTATAACCCCTCTCCAGGCCGGCTT25060.18872570984890646No Hit
ACCTGCCAGTAGGGAAGGATCCAGATTCCTGGGAATCCTATAAGCCCTCC25050.1886504003078654No Hit
ACCTGCCAGTAGGGAAGGATCCCTGTTATAACCCCTCTCCAGGCAGGCCT24840.18706889994600304No Hit
ACCTGCCAGTAGGGAAGGATCCCCGTTATAACCCCTCTCCAGGCAGGCTT23160.1744168970511043No Hit
ACCTGCCAGTAGGGAAGGATCATGAGGAGACCAGGAGGACATTCCACAGT21810.16425010901056067No Hit
ACCTGCCAGTAGGGAAGGATCCCTGTTATAACCCCTCTCCAGGCAGGCTC21250.16003277471226107No Hit
ACCTGCCAGTAGGGAAGGATCCCTGTTGTAACCCCTCTCCAGGCAGGCTT21080.158752512514563No Hit
ACCTGCCAGTAGGGAAGGATCATTCTCTTGAGAGCTTTCCCTTGTCTCTG20300.15287836831336No Hit
ACCTGCCAGTAGGGAAGGATCCCTGTCATAACCCCTCTCCAGGCAGGCTT19660.14805855768673187No Hit
ACCTGCCAGTAGGGAAGGATCCCTGTTATGACCCCTCTCCAGGCAGGCTT19580.14745608135840338No Hit
ACCTGCCAGTAGGGAAGGATCCCTGTTACAACCCCTCTCCAGGCAGGCTT19350.1457239619144589No Hit
ACCTGCCAGTAGGGAAGGATCCCTGTTATAACCCCTCTCCAGGCAGGCTA18390.13849424597451676No Hit
ACCTGCCAGTAGGGAAGGATCCCTGTTATAACCCCTCTCCAGGCTGGCTT18020.13570779295599739No Hit
ACCTGCCAGTAGGGAAGGATCCCTGTTTTAACCCCTCTCCAGGCAGGCTT17780.13390036397101185No Hit
ACCTGCCAGTAGGGAAGGATCCCTGTTATAACCCCTCTCCACGCAGGCTT17110.12885462472126055No Hit
ACCTGCCAGTAGGGAAGGACCCCTGTTATAACCCCTCTCCAGGCAGGCTT17110.12885462472126055No Hit
TCCTGCCAGTAGGGAAGGATCCCTGTTATAACCCCTCTCCAGGCAGGCTT16700.12576693353857693No Hit
ACCTGCCAGTAGGGAAGGATCCTATTGATTAAAATGTATTGGTCCTATTG15560.11718164585989563No Hit
ACCTGCCAGTAGGGAGGGATCCCTGTTATAACCCCTCTCCAGGCAGGCTT15190.11439519284137625No Hit
ACCTGCCAGTAGGGAAGGATCCCTGTTATAACCCCTCTCCACGCCGGCGT14250.10731609598351624No Hit
ACCTGCCAGTAGGGAAGGATCCCTGTTATTACCCCTCTCCAGGCAGGCTT14180.10678892919622879No Hit
ACCTGCCAGTAGGGAAGGATCCCTGTTATAACCCCACACCAGGCAGGCTT13340.10046292774877942No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)