FastQCFastQC Report
Thu 6 Jul 2023
D0_R2_VP6_S4_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameD0_R2_VP6_S4_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1567623
Total Bases92.4 Mbp
Sequences flagged as poor quality0
Sequence length59
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTGCCAGTAGGGAAGGATCCCTGTTATAACCCCTCTCCAGGCAGGCTT79757350.87785775023714No Hit
ACCTGCCAGTAGGGAAGGATCACAGGTGAGTCAAACAGGAAGCCTGCTCC103150.6580025937358663No Hit
ACCTGCCAGTAGGGAAGGATCCCTGTTATAACCCCTCTCCAGGCAGGCGT89330.5698436422532713No Hit
ACCTGCCAGTAGGGAAGGTGGGAAGAAATGAGTTCCCTGTACAATGTAAA79720.5085406376405551No Hit
ACCTGCCAGTAGGGAAGGATCCCTGTTATAACCCCACTCCAGGCAGGCTT57400.3661594656368272No Hit
ACCTGCCAGTAGGGAAGGATCCCTGTTATAACCCCTCTCCAGGCCGGCGT41350.26377515512339383No Hit
ACCTGCCAGTAGGGAAGGATCCCTGTTATAACCCCTCACCAGGCAGGCTT36110.23034875094330715No Hit
ACCTGCCAGTAGGGAAGGATCCCTGTTATAACCCCTCTCCAGGCGGGCTT31450.2006222159281919No Hit
ACCTGCCAGTAGGGAAGGATCATGAGGAGACCAGGAGGACATTCCACAGT30810.19653960167718898No Hit
ACCTGCCAGTAGGGAAGGATCCCTGTTATAACCCCTCTCCAGGCCGGCTT28730.18327110536142938No Hit
ACCTGCCAGTAGGGAAGGATCCCTGTTATAACCCCCCTCCAGGCAGGCTT25690.16387868766916536No Hit
ACCTGCCAGTAGGGAAGGATCCCTGTTATAACCCCTCCCCAGGCAGGCTT25610.16336836088779No Hit
ACCTGCCAGTAGGGAAGGATCCCTGCTATAACCCCTCTCCAGGCAGGCTT25290.1613270537622885No Hit
ACCTGCCAGTAGAGAAGGATCCCTGTTATAACCCCTCTCCAGGCAGGCTT24660.1573082303589575No Hit
ACCTGCCAGTAGGGAAGGATCCCTGTTATAACCCCTCTCCGGGCAGGCTT21830.13925542046780381No Hit
ACCTGCCAGTAGGGAAGGATCCCTGTTATAACCCCTCTCCACGCAGGCTT20710.13211084552854863No Hit
ACCTGCCAGTAGGGAAGGATCCCTGTTATAACCCCTCTCCAGGCAGGCTA20410.130197120098391No Hit
ACCTGCCAGTAGGGAAGGATCCCCGTTATAACCCCTCTCCAGGCAGGCTT20410.130197120098391No Hit
ACCTGCCAGTAGGGAAGGATCCCTGTTATAGCCCCTCTCCAGGCAGGCTT20370.1299419567077033No Hit
ACCTGCCAGTAGGGAAGGATCTCATTGAATTTCTTTAAAGGTGGAATCTC19890.1268799960194511No Hit
ACCTGCCAGTAGGGAAGGATCCCTGTTATAACCCCTCTCCAGGCAGGCCT19710.12573176076135653No Hit
ACCTGCCAGTAGGGAAGGATCCCTGTTTTAACCCCTCTCCAGGCAGGCTT19300.12311633600680776No Hit
ACCTGCCAGTAGGGAAGGACCCCTGTTATAACCCCTCTCCAGGCAGGCTT19180.12235084583474472No Hit
ACCTGCCAGTAGGGAGGGATCCCTGTTATAACCCCTCTCCAGGCAGGCTT18670.11909751260347672No Hit
ACCTGCCAGTAGGGAAGGATCCCTGTTATAACCCCTCTCCAGGCAGGCTC18440.11763032310702255No Hit
ACCTGCCAGTAGGGAAGGATCCCTGTTATAACCCCTCTCCAGGCTGGCTT17820.11367529055136344No Hit
ACCTGCCAGTAGGGAAGGATCCCTGTTGTAACCCCTCTCCAGGCAGGCTT17710.11297359122697231No Hit
ACCTGCCAGTAGGGAAGGATCGCTGTTATAACCCCTCTCCAGGCAGGCTT17200.10972025799570431No Hit
ACCTGCCAGTAGGGAAGGATCCCTGTTATAACCCCTCTCCACGCCGGCGT16670.10633934306909251No Hit
ACCTGCCAGTAGGGAAGGATCCCTGTTACAACCCCTCTCCAGGCAGGCTT16180.10321359153316836No Hit
ACCTGCCAGTAGGGAAGGATCCCTGTTATAACCCCACACCAGGCAGGCTT16040.10232051966576147No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)