## htsjdk.samtools.metrics.StringHeader # MarkDuplicates MAX_FILE_HANDLES_FOR_READ_ENDS_MAP=999 INPUT=[/ceph/rimlsfnwi/data/moldevbio/share_moldevbio/ghe_2022/results/bwa-mem2/GRCh38-RU_1uM_2.samtools-coordinate-unsieved.bam] OUTPUT=/ceph/rimlsfnwi/data/moldevbio/share_moldevbio/ghe_2022/results/bwa-mem2/GRCh38-RU_1uM_2.samtools-coordinate-dupmarked.bam METRICS_FILE=/ceph/rimlsfnwi/data/moldevbio/share_moldevbio/ghe_2022/results/qc/markdup/GRCh38-RU_1uM_2.samtools-coordinate.metrics.txt TMP_DIR=[/scratch/slrinzema/tmp] MAX_SEQUENCES_FOR_DISK_READ_ENDS_MAP=50000 SORTING_COLLECTION_SIZE_RATIO=0.25 TAG_DUPLICATE_SET_MEMBERS=false REMOVE_SEQUENCING_DUPLICATES=false TAGGING_POLICY=DontTag CLEAR_DT=true DUPLEX_UMI=false ADD_PG_TAG_TO_READS=true REMOVE_DUPLICATES=false ASSUME_SORTED=false DUPLICATE_SCORING_STRATEGY=SUM_OF_BASE_QUALITIES PROGRAM_RECORD_ID=MarkDuplicates PROGRAM_GROUP_NAME=MarkDuplicates READ_NAME_REGEX= OPTICAL_DUPLICATE_PIXEL_DISTANCE=100 MAX_OPTICAL_DUPLICATE_SET_SIZE=300000 VERBOSITY=INFO QUIET=false VALIDATION_STRINGENCY=STRICT COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false GA4GH_CLIENT_SECRETS=client_secrets.json USE_JDK_DEFLATER=false USE_JDK_INFLATER=false ## htsjdk.samtools.metrics.StringHeader # Started on: Thu Sep 22 17:43:35 CEST 2022 ## METRICS CLASS picard.sam.DuplicationMetrics LIBRARY UNPAIRED_READS_EXAMINED READ_PAIRS_EXAMINED SECONDARY_OR_SUPPLEMENTARY_RDS UNMAPPED_READS UNPAIRED_READ_DUPLICATES READ_PAIR_DUPLICATES READ_PAIR_OPTICAL_DUPLICATES PERCENT_DUPLICATION ESTIMATED_LIBRARY_SIZE Unknown Library 972499 41672408 0 1187981 701869 6934751 0 0.172816 110894635 ## HISTOGRAM java.lang.Double BIN CoverageMult all_sets non_optical_sets 1.0 1 31968941 31968941 2.0 1.686751 1816063 1816063 3.0 2.158377 409111 409111 4.0 2.482267 181188 181188 5.0 2.704699 100663 100663 6.0 2.857454 62037 62037 7.0 2.962359 41671 41671 8.0 3.034402 29262 29262 9.0 3.083878 21627 21627 10.0 3.117856 16216 16216 11.0 3.14119 12904 12904 12.0 3.157215 10104 10104 13.0 3.16822 8078 8078 14.0 3.175777 6633 6633 15.0 3.180968 5509 5509 16.0 3.184532 4729 4729 17.0 3.18698 3920 3920 18.0 3.188661 3429 3429 19.0 3.189815 2934 2934 20.0 3.190608 2495 2495 21.0 3.191153 2293 2293 22.0 3.191527 2005 2005 23.0 3.191783 1698 1698 24.0 3.19196 1551 1551 25.0 3.192081 1391 1391 26.0 3.192164 1303 1303 27.0 3.192221 1143 1143 28.0 3.19226 1045 1045 29.0 3.192287 939 939 30.0 3.192306 862 862 31.0 3.192319 833 833 32.0 3.192327 714 714 33.0 3.192333 740 740 34.0 3.192337 626 626 35.0 3.19234 594 594 36.0 3.192342 489 489 37.0 3.192343 521 521 38.0 3.192344 468 468 39.0 3.192345 475 475 40.0 3.192345 425 425 41.0 3.192346 392 392 42.0 3.192346 357 357 43.0 3.192346 323 323 44.0 3.192346 324 324 45.0 3.192346 307 307 46.0 3.192346 301 301 47.0 3.192346 276 276 48.0 3.192346 224 224 49.0 3.192346 243 243 50.0 3.192346 240 240 51.0 3.192346 236 236 52.0 3.192346 188 188 53.0 3.192346 176 176 54.0 3.192346 176 176 55.0 3.192346 185 185 56.0 3.192346 158 158 57.0 3.192346 139 139 58.0 3.192346 151 151 59.0 3.192346 146 146 60.0 3.192346 140 140 61.0 3.192346 155 155 62.0 3.192346 138 138 63.0 3.192346 117 117 64.0 3.192346 107 107 65.0 3.192346 103 103 66.0 3.192346 113 113 67.0 3.192346 109 109 68.0 3.192346 107 107 69.0 3.192346 105 105 70.0 3.192346 109 109 71.0 3.192346 99 99 72.0 3.192346 96 96 73.0 3.192346 86 86 74.0 3.192346 95 95 75.0 3.192346 69 69 76.0 3.192346 80 80 77.0 3.192346 95 95 78.0 3.192346 65 65 79.0 3.192346 67 67 80.0 3.192346 74 74 81.0 3.192346 59 59 82.0 3.192346 63 63 83.0 3.192346 48 48 84.0 3.192346 61 61 85.0 3.192346 50 50 86.0 3.192346 47 47 87.0 3.192346 63 63 88.0 3.192346 61 61 89.0 3.192346 64 64 90.0 3.192346 41 41 91.0 3.192346 45 45 92.0 3.192346 49 49 93.0 3.192346 33 33 94.0 3.192346 55 55 95.0 3.192346 43 43 96.0 3.192346 44 44 97.0 3.192346 48 48 98.0 3.192346 49 49 99.0 3.192346 38 38 100.0 3.192346 36 36 101.0 0 34 34 102.0 0 30 30 103.0 0 43 43 104.0 0 24 24 105.0 0 39 39 106.0 0 20 20 107.0 0 30 30 108.0 0 34 34 109.0 0 28 28 110.0 0 28 28 111.0 0 31 31 112.0 0 34 34 113.0 0 33 33 114.0 0 23 23 115.0 0 29 29 116.0 0 23 23 117.0 0 18 18 118.0 0 26 26 119.0 0 19 19 120.0 0 28 28 121.0 0 23 23 122.0 0 21 21 123.0 0 20 20 124.0 0 26 26 125.0 0 33 33 126.0 0 21 21 127.0 0 16 16 128.0 0 18 18 129.0 0 14 14 130.0 0 23 23 131.0 0 11 11 132.0 0 22 22 133.0 0 18 18 134.0 0 15 15 135.0 0 23 23 136.0 0 21 21 137.0 0 18 18 138.0 0 16 16 139.0 0 23 23 140.0 0 18 18 141.0 0 17 17 142.0 0 22 22 143.0 0 16 16 144.0 0 10 10 145.0 0 17 17 146.0 0 18 18 147.0 0 14 14 148.0 0 20 20 149.0 0 12 12 150.0 0 7 7 151.0 0 13 13 152.0 0 9 9 153.0 0 13 13 154.0 0 12 12 155.0 0 16 16 156.0 0 12 12 157.0 0 13 13 158.0 0 11 11 159.0 0 20 20 160.0 0 8 8 161.0 0 9 9 162.0 0 7 7 163.0 0 12 12 164.0 0 5 5 165.0 0 9 9 166.0 0 12 12 167.0 0 13 13 168.0 0 11 11 169.0 0 11 11 170.0 0 9 9 171.0 0 13 13 172.0 0 8 8 173.0 0 13 13 174.0 0 8 8 175.0 0 9 9 176.0 0 4 4 177.0 0 6 6 178.0 0 8 8 179.0 0 10 10 180.0 0 8 8 181.0 0 13 13 182.0 0 7 7 183.0 0 8 8 184.0 0 10 10 185.0 0 6 6 186.0 0 8 8 187.0 0 9 9 188.0 0 9 9 189.0 0 9 9 190.0 0 8 8 191.0 0 3 3 192.0 0 6 6 193.0 0 10 10 194.0 0 4 4 195.0 0 11 11 196.0 0 7 7 197.0 0 4 4 198.0 0 7 7 199.0 0 7 7 200.0 0 7 7 201.0 0 8 8 202.0 0 5 5 203.0 0 7 7 204.0 0 9 9 205.0 0 3 3 206.0 0 9 9 207.0 0 8 8 208.0 0 8 8 209.0 0 5 5 210.0 0 4 4 211.0 0 3 3 212.0 0 5 5 213.0 0 5 5 214.0 0 9 9 215.0 0 7 7 216.0 0 7 7 217.0 0 7 7 218.0 0 9 9 219.0 0 7 7 220.0 0 13 13 221.0 0 6 6 222.0 0 7 7 223.0 0 5 5 224.0 0 3 3 225.0 0 3 3 226.0 0 5 5 227.0 0 6 6 228.0 0 7 7 229.0 0 7 7 230.0 0 9 9 231.0 0 4 4 232.0 0 2 2 233.0 0 8 8 234.0 0 7 7 235.0 0 4 4 236.0 0 6 6 237.0 0 4 4 238.0 0 5 5 239.0 0 5 5 240.0 0 2 2 241.0 0 4 4 242.0 0 6 6 243.0 0 8 8 244.0 0 3 3 245.0 0 5 5 246.0 0 3 3 247.0 0 4 4 248.0 0 3 3 249.0 0 3 3 250.0 0 1 1 251.0 0 4 4 252.0 0 6 6 253.0 0 4 4 254.0 0 8 8 255.0 0 4 4 256.0 0 2 2 257.0 0 8 8 258.0 0 1 1 259.0 0 4 4 260.0 0 5 5 261.0 0 5 5 262.0 0 2 2 263.0 0 2 2 264.0 0 3 3 265.0 0 3 3 266.0 0 2 2 267.0 0 6 6 268.0 0 5 5 269.0 0 5 5 270.0 0 4 4 271.0 0 1 1 272.0 0 5 5 273.0 0 3 3 274.0 0 3 3 275.0 0 2 2 276.0 0 2 2 277.0 0 2 2 278.0 0 3 3 279.0 0 2 2 280.0 0 2 2 281.0 0 3 3 282.0 0 2 2 283.0 0 2 2 284.0 0 1 1 285.0 0 3 3 286.0 0 3 3 287.0 0 3 3 288.0 0 3 3 289.0 0 2 2 290.0 0 3 3 291.0 0 2 2 292.0 0 4 4 293.0 0 6 6 294.0 0 3 3 296.0 0 1 1 297.0 0 3 3 298.0 0 4 4 299.0 0 1 1 300.0 0 4 4 301.0 0 2 2 302.0 0 1 1 303.0 0 2 2 304.0 0 3 3 305.0 0 1 1 306.0 0 4 4 307.0 0 2 2 308.0 0 2 2 309.0 0 1 1 310.0 0 2 2 311.0 0 2 2 312.0 0 4 4 313.0 0 4 4 314.0 0 3 3 315.0 0 2 2 316.0 0 1 1 317.0 0 3 3 318.0 0 3 3 319.0 0 2 2 320.0 0 1 1 321.0 0 1 1 322.0 0 1 1 323.0 0 4 4 324.0 0 4 4 325.0 0 2 2 326.0 0 1 1 328.0 0 2 2 329.0 0 3 3 330.0 0 1 1 331.0 0 1 1 332.0 0 1 1 334.0 0 2 2 335.0 0 1 1 336.0 0 1 1 338.0 0 1 1 341.0 0 3 3 342.0 0 1 1 343.0 0 1 1 344.0 0 1 1 345.0 0 3 3 348.0 0 3 3 349.0 0 1 1 350.0 0 1 1 355.0 0 1 1 356.0 0 1 1 358.0 0 1 1 359.0 0 3 3 362.0 0 1 1 363.0 0 3 3 364.0 0 2 2 365.0 0 1 1 367.0 0 1 1 370.0 0 2 2 371.0 0 2 2 372.0 0 3 3 373.0 0 1 1 374.0 0 3 3 375.0 0 2 2 376.0 0 3 3 377.0 0 2 2 378.0 0 1 1 379.0 0 1 1 380.0 0 2 2 381.0 0 1 1 382.0 0 2 2 387.0 0 1 1 392.0 0 2 2 393.0 0 1 1 394.0 0 1 1 397.0 0 1 1 398.0 0 2 2 399.0 0 1 1 401.0 0 1 1 402.0 0 1 1 403.0 0 2 2 404.0 0 1 1 406.0 0 3 3 407.0 0 2 2 408.0 0 3 3 409.0 0 1 1 412.0 0 1 1 413.0 0 2 2 415.0 0 1 1 417.0 0 1 1 420.0 0 2 2 423.0 0 1 1 429.0 0 1 1 431.0 0 1 1 433.0 0 2 2 434.0 0 1 1 438.0 0 1 1 439.0 0 1 1 442.0 0 1 1 444.0 0 1 1 446.0 0 1 1 447.0 0 1 1 448.0 0 1 1 453.0 0 1 1 456.0 0 1 1 458.0 0 2 2 460.0 0 1 1 466.0 0 1 1 467.0 0 1 1 471.0 0 1 1 473.0 0 1 1 474.0 0 1 1 477.0 0 2 2 479.0 0 1 1 481.0 0 2 2 484.0 0 1 1 486.0 0 3 3 492.0 0 1 1 495.0 0 1 1 505.0 0 1 1 508.0 0 1 1 517.0 0 1 1 520.0 0 1 1 521.0 0 4 4 522.0 0 2 2 523.0 0 1 1 527.0 0 1 1 528.0 0 1 1 529.0 0 1 1 532.0 0 1 1 534.0 0 2 2 537.0 0 1 1 539.0 0 1 1 543.0 0 1 1 545.0 0 1 1 550.0 0 1 1 551.0 0 2 2 552.0 0 2 2 555.0 0 1 1 556.0 0 1 1 558.0 0 1 1 560.0 0 1 1 562.0 0 2 2 565.0 0 1 1 571.0 0 1 1 578.0 0 1 1 580.0 0 2 2 581.0 0 1 1 582.0 0 1 1 583.0 0 1 1 586.0 0 1 1 589.0 0 1 1 591.0 0 1 1 596.0 0 1 1 597.0 0 1 1 598.0 0 1 1 602.0 0 1 1 606.0 0 2 2 608.0 0 1 1 609.0 0 1 1 613.0 0 2 2 617.0 0 1 1 621.0 0 1 1 626.0 0 1 1 628.0 0 1 1 631.0 0 1 1 640.0 0 1 1 648.0 0 1 1 654.0 0 1 1 658.0 0 1 1 664.0 0 1 1 666.0 0 1 1 669.0 0 1 1 675.0 0 1 1 682.0 0 1 1 685.0 0 1 1 686.0 0 1 1 697.0 0 2 2 698.0 0 1 1 701.0 0 1 1 709.0 0 1 1 711.0 0 1 1 718.0 0 1 1 720.0 0 1 1 723.0 0 1 1 724.0 0 1 1 730.0 0 1 1 731.0 0 1 1 733.0 0 1 1 752.0 0 1 1 764.0 0 1 1 769.0 0 1 1 775.0 0 1 1 778.0 0 1 1 789.0 0 2 2 790.0 0 2 2 793.0 0 1 1 799.0 0 1 1 823.0 0 1 1 830.0 0 1 1 833.0 0 1 1 837.0 0 1 1 851.0 0 1 1 861.0 0 1 1 862.0 0 1 1 869.0 0 1 1 877.0 0 1 1 882.0 0 1 1 885.0 0 1 1 887.0 0 1 1 889.0 0 1 1 895.0 0 1 1 897.0 0 1 1 898.0 0 1 1 899.0 0 1 1 909.0 0 1 1 917.0 0 1 1 922.0 0 1 1 940.0 0 1 1 947.0 0 1 1 949.0 0 1 1 990.0 0 1 1 1012.0 0 1 1 1028.0 0 1 1 1035.0 0 1 1 1045.0 0 1 1 1053.0 0 1 1 1078.0 0 1 1 1120.0 0 1 1 1121.0 0 1 1 1141.0 0 1 1 1149.0 0 1 1 1171.0 0 1 1 1206.0 0 1 1 1265.0 0 1 1 1270.0 0 1 1 1319.0 0 1 1 1470.0 0 1 1 1561.0 0 1 1 1562.0 0 1 1 1694.0 0 1 1