The samples file used for this run:
sample | assembly | technical_replicates | descriptivate_name |
---|---|---|---|
HSCD12_DMSO_1bio | GRCh38.p13 | DMSO_1 | HSCD12_DMSO_1bio |
HSCD12_DMSO_1tec | GRCh38.p13 | DMSO_1 | HSCD12_DMSO_1tec |
HSCD12_DMSO_2bio | GRCh38.p13 | DMSO_2 | HSCD12_DMSO_2bio |
HSCD12_DMSO_2tec | GRCh38.p13 | DMSO_2 | HSCD12_DMSO_2tec |
HSCD12_DMSO_3bio | GRCh38.p13 | DMSO_3 | HSCD12_DMSO_3bio |
HSCD12_DMSO_3tec | GRCh38.p13 | DMSO_3 | HSCD12_DMSO_3tec |
HSCD12_RU1uM_1bio | GRCh38.p13 | RU1uM_1 | HSCD12_RU1uM_1bio |
HSCD12_RU1uM_1tec | GRCh38.p13 | RU1uM_1 | HSCD12_RU1uM_1tec |
HSCD12_RU1uM_2bio | GRCh38.p13 | RU1uM_2 | HSCD12_RU1uM_2bio |
HSCD12_RU1uM_2tec | GRCh38.p13 | RU1uM_2 | HSCD12_RU1uM_2tec |
HSCD12_RU1uM_3bio | GRCh38.p13 | RU1uM_3 | HSCD12_RU1uM_3bio |
HSCD12_RU1uM_3tec | GRCh38.p13 | RU1uM_3 | HSCD12_RU1uM_3tec |
HSCD12_RU1uMTA100nM_1bio | GRCh38.p13 | RU1uMTA100nM_1 | HSCD12_RU1uMTA100nM_1bio |
HSCD12_RU1uMTA100nM_1tec | GRCh38.p13 | RU1uMTA100nM_1 | HSCD12_RU1uMTA100nM_1tec |
HSCD12_RU1uMTA100nM_2bio | GRCh38.p13 | RU1uMTA100nM_2 | HSCD12_RU1uMTA100nM_2bio |
HSCD12_RU1uMTA100nM_2tec | GRCh38.p13 | RU1uMTA100nM_2 | HSCD12_RU1uMTA100nM_2tec |
HSCD12_RU1uMTA100nM_3bio | GRCh38.p13 | RU1uMTA100nM_3 | HSCD12_RU1uMTA100nM_3bio |
HSCD12_RU1uMTA100nM_3tec | GRCh38.p13 | RU1uMTA100nM_3 | HSCD12_RU1uMTA100nM_3tec |
HSCD12_RU1uMTA1uM_1bio | GRCh38.p13 | RU1uMTA1uM_1 | HSCD12_RU1uMTA1uM_1bio |
HSCD12_RU1uMTA1uM_1tec | GRCh38.p13 | RU1uMTA1uM_1 | HSCD12_RU1uMTA1uM_1tec |
HSCD12_RU1uMTA1uM_2bio | GRCh38.p13 | RU1uMTA1uM_2 | HSCD12_RU1uMTA1uM_2bio |
HSCD12_RU1uMTA1uM_2tec | GRCh38.p13 | RU1uMTA1uM_2 | HSCD12_RU1uMTA1uM_2tec |
HSCD12_RU1uMTA1uM_3bio | GRCh38.p13 | RU1uMTA1uM_3 | HSCD12_RU1uMTA1uM_3bio |
HSCD12_RU1uMTA1uM_3tec | GRCh38.p13 | RU1uMTA1uM_3 | HSCD12_RU1uMTA1uM_3tec |
HSCD12_TA100nM_1bio | GRCh38.p13 | TA100nM_1 | HSCD12_TA100nM_1bio |
HSCD12_TA100nM_1tec | GRCh38.p13 | TA100nM_1 | HSCD12_TA100nM_1tec |
HSCD12_TA100nM_2bio | GRCh38.p13 | TA100nM_2 | HSCD12_TA100nM_2bio |
HSCD12_TA100nM_2tec | GRCh38.p13 | TA100nM_2 | HSCD12_TA100nM_2tec |
HSCD12_TA100nM_3bio | GRCh38.p13 | TA100nM_3 | HSCD12_TA100nM_3bio |
HSCD12_TA100nM_3tec | GRCh38.p13 | TA100nM_3 | HSCD12_TA100nM_3tec |
HSCD12_TA1uM_1bio | GRCh38.p13 | TA1uM_1 | HSCD12_TA1uM_1bio |
HSCD12_TA1uM_1tec | GRCh38.p13 | TA1uM_1 | HSCD12_TA1uM_1tec |
HSCD12_TA1uM_2bio | GRCh38.p13 | TA1uM_2 | HSCD12_TA1uM_2bio |
HSCD12_TA1uM_2tec | GRCh38.p13 | TA1uM_2 | HSCD12_TA1uM_2tec |
HSCD12_TA1uM_3bio | GRCh38.p13 | TA1uM_3 | HSCD12_TA1uM_3bio |
HSCD12_TA1uM_3tec | GRCh38.p13 | TA1uM_3 | HSCD12_TA1uM_3tec |
NP_Day12_supernatant_DMSO_1 | GRCh38.p13 | DMSO_4 | NP_Day12_supernatant_DMSO_1 |
NP_Day12_supernatant_DMSO_2 | GRCh38.p13 | DMSO_4 | NP_Day12_supernatant_DMSO_2 |
NP_Day12_supernatant_DMSO_3 | GRCh38.p13 | DMSO_4 | NP_Day12_supernatant_DMSO_3 |
NP_Day12_supernatant_TA_1 | GRCh38.p13 | TA_1 | NP_Day12_supernatant_TA_1 |
NP_Day12_supernatant_TA_2 | GRCh38.p13 | TA_1 | NP_Day12_supernatant_TA_2 |
NP_Day12_supernatant_TA_3 | GRCh38.p13 | TA_1 | NP_Day12_supernatant_TA_3 |
NP_Day1_adherentcells_DMSO_1 | GRCh38.p13 | day_1_dmso | NP_Day1_adherentcells_DMSO_1 |
NP_Day1_adherentcells_DMSO_2 | GRCh38.p13 | day_1_dmso | NP_Day1_adherentcells_DMSO_2 |
NP_Day1_adherentcells_DMSO_3 | GRCh38.p13 | day_1_dmso | NP_Day1_adherentcells_DMSO_3 |
NP_Day1_adherentcells_TA_1 | GRCh38.p13 | day_1_TA | NP_Day1_adherentcells_TA_1 |
NP_Day1_adherentcells_TA_2 | GRCh38.p13 | day_1_TA | NP_Day1_adherentcells_TA_2 |
NP_Day1_adherentcells_TA_3 | GRCh38.p13 | day_1_TA | NP_Day1_adherentcells_TA_3 |
# tab-separated file of the samples
samples: samples.tsv
# pipeline file locations
result_dir: ./results # where to store results
genome_dir: /ceph/rimlsfnwi/data/genomes/
fastq_dir: /home/slrinzema/ceph/ghe/samples
# contact info for multiqc report and trackhub
email: slrinzema@science.ru.nl
# produce a UCSC trackhub?
create_trackhub: true
# how to handle replicates
technical_replicates: merge # change to "keep" to not combine them
# which trimmer to use
trimmer: fastp
# which quantifier to use
quantifier: salmon
# which aligner to use
aligner: star
# how to sort bam
bam_sorter:
samtools:
coordinate
# filtering after alignment
remove_blacklist: true
only_primary_align: true
min_mapping_quality: 255 # instead of 30
remove_dups: false