## htsjdk.samtools.metrics.StringHeader # MarkDuplicates MAX_FILE_HANDLES_FOR_READ_ENDS_MAP=999 INPUT=[/ceph/rimlsfnwi/data/moldevbio/heeringen/slrinzema/ghe/2023_s2s_run/results/star/GRCh38.p13-RU1uMTA100nM_1.samtools-coordinate-unsieved.bam] OUTPUT=/ceph/rimlsfnwi/data/moldevbio/heeringen/slrinzema/ghe/2023_s2s_run/results/star/GRCh38.p13-RU1uMTA100nM_1.samtools-coordinate-dupmarked.bam METRICS_FILE=/ceph/rimlsfnwi/data/moldevbio/heeringen/slrinzema/ghe/2023_s2s_run/results/qc/markdup/GRCh38.p13-RU1uMTA100nM_1.samtools-coordinate.metrics.txt TMP_DIR=[/scratch/slrinzema/tmp] MAX_SEQUENCES_FOR_DISK_READ_ENDS_MAP=50000 SORTING_COLLECTION_SIZE_RATIO=0.25 TAG_DUPLICATE_SET_MEMBERS=false REMOVE_SEQUENCING_DUPLICATES=false TAGGING_POLICY=DontTag CLEAR_DT=true DUPLEX_UMI=false FLOW_MODE=false FLOW_QUALITY_SUM_STRATEGY=false USE_END_IN_UNPAIRED_READS=false USE_UNPAIRED_CLIPPED_END=false UNPAIRED_END_UNCERTAINTY=0 FLOW_SKIP_FIRST_N_FLOWS=0 FLOW_Q_IS_KNOWN_END=false FLOW_EFFECTIVE_QUALITY_THRESHOLD=15 ADD_PG_TAG_TO_READS=true REMOVE_DUPLICATES=false ASSUME_SORTED=false DUPLICATE_SCORING_STRATEGY=SUM_OF_BASE_QUALITIES PROGRAM_RECORD_ID=MarkDuplicates PROGRAM_GROUP_NAME=MarkDuplicates READ_NAME_REGEX= OPTICAL_DUPLICATE_PIXEL_DISTANCE=100 MAX_OPTICAL_DUPLICATE_SET_SIZE=300000 VERBOSITY=INFO QUIET=false VALIDATION_STRINGENCY=STRICT COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false USE_JDK_DEFLATER=false USE_JDK_INFLATER=false ## htsjdk.samtools.metrics.StringHeader # Started on: Wed Oct 04 10:21:19 CEST 2023 ## METRICS CLASS picard.sam.DuplicationMetrics LIBRARY UNPAIRED_READS_EXAMINED READ_PAIRS_EXAMINED SECONDARY_OR_SUPPLEMENTARY_RDS UNMAPPED_READS UNPAIRED_READ_DUPLICATES READ_PAIR_DUPLICATES READ_PAIR_OPTICAL_DUPLICATES PERCENT_DUPLICATION ESTIMATED_LIBRARY_SIZE Unknown Library 931 43132414 18945077 0 748 6900133 0 0.159983 120011890 ## HISTOGRAM java.lang.Double BIN CoverageMult all_sets non_optical_sets 1.0 1 33239016 33239016 2.0 1.698094 2114492 2114492 3.0 2.18543 394192 394192 4.0 2.525636 159775 159775 5.0 2.763132 87220 87220 6.0 2.928926 53507 53507 7.0 3.044667 36175 36175 8.0 3.125464 25915 25915 9.0 3.181869 19112 19112 10.0 3.221244 14910 14910 11.0 3.248732 11572 11572 12.0 3.267921 9456 9456 13.0 3.281317 7599 7599 14.0 3.290668 6195 6195 15.0 3.297197 5364 5364 16.0 3.301754 4580 4580 17.0 3.304935 3880 3880 18.0 3.307156 3332 3332 19.0 3.308707 3007 3007 20.0 3.309789 2553 2553 21.0 3.310545 2302 2302 22.0 3.311072 2039 2039 23.0 3.31144 1825 1825 24.0 3.311697 1592 1592 25.0 3.311877 1412 1412 26.0 3.312002 1268 1268 27.0 3.31209 1226 1226 28.0 3.312151 1133 1133 29.0 3.312193 1028 1028 30.0 3.312223 903 903 31.0 3.312244 790 790 32.0 3.312258 756 756 33.0 3.312268 685 685 34.0 3.312276 675 675 35.0 3.31228 597 597 36.0 3.312284 545 545 37.0 3.312286 493 493 38.0 3.312288 432 432 39.0 3.312289 400 400 40.0 3.31229 396 396 41.0 3.312291 402 402 42.0 3.312291 398 398 43.0 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