[stdout]

starting vdj_asm_asm, mem = 0.01
available mem = 828.30 GB
/scratch/etanis/software/cellranger-6.1.2/lib/bin/cr_vdj martian assembly main /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_T_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk84-ucc6a7a72e6 /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_T_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk84-ucc6a7a72e6/files /scratch/etanis/ST-118/multi/dimitri-multi/journal/SC_MULTI_CS.SC_MULTI_CORE.MULTI_GEM_WELL_PROCESSOR.VDJ_T_GEM_WELL_PROCESSOR.SC_VDJ_CONTIG_ASSEMBLER.ASSEMBLE_VDJ.fork0.chnk84.ucc6a7a72e6
n50_n50_rpu = 2159

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.0 = TCCCGATTCGCCAAAT-1

using 40565 reads

====================================================================================

graph has 12925 edges initially, 166 edges after simplification

total ucounts = 722
nonsolo ucounts = 514[2^83, 3^57, 4^53, 5^35, 6^26, 7^29, 8^15, 9^28, 10^18, 11^14, 12^13, 13^14, 14^12, 15^14, 16^8, 17^6, 18^4, 19^7, 20^3, 21^2, 22^3, 23^2, 24^4, 25^7, 26^2, 27^5, 28^3, 29^4, 30^3, 33, 34^2, 35^2, 36, 38^2, 39, 42^2, 43, 44, 48, 49, 50^2, 51, 54, 83, 89, 92, 101, 165, 321, 360, 500, 757, 771, 1598, 1640, 1666, 1724, 1932, 2645, 2768, 2838, 2907, 3278, 9425]
surviving nonsolo ucounts = 15[92, 500, 757, 771, 1598, 1640, 1666, 1724, 1932, 2645, 2768, 2838, 2907, 3278, 9425]
ids = [144, 112, 688, 459, 16, 183, 209, 519, 673, 463, ...]

====================================================================================

UMI info for barcode TCCCGATTCGCCAAAT-1 contig 1 = AAGCACTCTT...
umi AACCGACTAC = 1612 reads: +394 validated
umi ATCCTTAGGC = 92 reads: +394 validated
umi CAACGCGCAC = 1649 reads: +394 validated
umi GTAGACCGGG = 770 reads: +394 validated

UMI info for barcode TCCCGATTCGCCAAAT-1 contig 2 = ATTCTTTCTT...
umi AAAGTCCGCA = 2769 reads: +397 validated
umi CAGCTCTTAT = 1658 reads: +397 validated
umi CGTTTCCACT = 3291 reads: +397 validated
umi GTATACGCTC = 2655 reads: +397 validated
umi TACTCCGAGG = 1720 reads: +397 validated
umi TCATCAGCTG = 2849 reads: +397 validated
umi TTATTAGGGA = 1980 reads: +111 -1XX +285 invalidated

GOOD CONTIGS

TIG 1[bases=533]
0-97 ==> 0-97 on |492|TRAV27|5'UTR| [len=97] (mis=0)
97-425 ==> 0-328 on |493|TRAV27|L-REGION+V-REGION| [len=328] (mis=3)
436-491 ==> 0-55 on |406|TRAJ21|J-REGION| [len=55] (mis=3)
491-533 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 4 umis using 199 reads
cdr3 = CAGRSYDGSFNKFYF at 415, score = 10 + 7
umis assigned: [16, 144, 183, 459]
of which 4 are surviving nonsolos
reads assigned: 4054
start codons at 70, 79, 97, 141, 249, 350, 431, 479
confident = true

TIG 2[bases=469]
0-20 ==> 0-20 on |612|TRBV28|5'UTR| [len=20] (mis=0)
20-356 ==> 0-336 on |613|TRBV28|L-REGION+V-REGION| [len=344] (mis=0)
375-417 ==> 8-50 on |540|TRBJ1-5|J-REGION| [len=50] (mis=0)
417-469 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 7 umis using 1267 reads
cdr3 = CASLRSGSVEPQHF at 347, score = 6 + 8
umis assigned: [8, 209, 315, 463, 519, 568, 673]
of which 7 are surviving nonsolos
reads assigned: 16617
start codons at 20, 78, 142, 155, 162, 170, 225, 228, 236, 338, 393
confident = true

REJECT CONTIGS

TIG 1[bases=548]
7-92 ==> 4758-4843 on segment before TRBV6-7 exon 1 [len=4843] (mis=2)
7-92 ==> 6762-6847 on segment before TRBV6-8 exon 1 [len=6847] (mis=2)
92-436 ==> 0-344 on |669|TRBV6-9|L-REGION+V-REGION| [len=344] (mis=4)
446-496 ==> 0-50 on |540|TRBJ1-5|J-REGION| [len=50] (mis=2)
496-548 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
umis assigned: [68, 112, 197, 293, 525, 688]
of which 4 are surviving nonsolos
reads assigned: 11552
start codons at 15, 50, 92, 150, 203, 227, 234, 269, 333, 342, 472
confident = false
did not find CDR3
now this is a cell
paired!

CCTGGTGATACAGGCCTCTACCTCTGTGCAGGGCGTTCCTATGACGGCAGCTTCAACAAATTTTACTTTGGATCTGGGACCAAACTCAATGTAAAACCAA <==> GCCAGCACCAACCAGACATCTATGTACCTCTGTGCCAGCCTCCGTAGCGGGTCGGTGGAGCCCCAGCATTTTGGTGATGGGACTCGACTCTCCATCCTAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.6 = TCCCGATTCGGTCTAA-1

using 641 reads

====================================================================================

graph has 304 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 6[2^2, 4^3, 622]
surviving nonsolo ucounts = 1[622]
ids = [2]

====================================================================================

UMI info for barcode TCCCGATTCGGTCTAA-1 contig 1 = GTGGATAGGG...
umi GATAATGCTT = 628 reads: +397 validated

GOOD CONTIGS

TIG 1[bases=640]
0-201 ==> 19-220 on |525|TRAV8-6|5'UTR| [len=220] (mis=1)
201-542 ==> 0-341 on |526|TRAV8-6|L-REGION+V-REGION| [len=341] (mis=5)
538-598 ==> 0-60 on |414|TRAJ29|J-REGION| [len=60] (mis=0)
598-640 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 1 umis using 10 reads
cdr3 = CAVWNSGNTPLVF at 528, score = 7 + 9
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 614
start codons at 201, 364
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.7 = TCCCGATTCGGTTAAC-1

using 83 reads

====================================================================================

graph has 60 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 8[2^3, 3^2, 7, 14, 48]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.16 = TCCCGATTCTAGAGTC-1

using 35 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.17 = TCCCGATTCTCCAGGG-1

using 26 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[3, 22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.18 = TCCCGATTCTCCCTGA-1

using 69 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[69]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.20 = TCCCGATTCTCGTATT-1

using 203 reads

====================================================================================

graph has 154 edges initially, 4 edges after simplification

total ucounts = 13
nonsolo ucounts = 11[2, 3, 4^2, 12, 15, 18, 20, 24, 38, 61]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.27 = TCCCGATTCTGTCAAG-1

using 65 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[65]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.28 = TCCCGATTCTGTTGAG-1

using 60 reads

====================================================================================

graph has 56 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 4[2, 6^2, 40]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.29 = TCCCGATTCTGTTTGT-1

using 15720 reads

====================================================================================

graph has 11848 edges initially, 406 edges after simplification

total ucounts = 1463
nonsolo ucounts = 1102[2^175, 3^105, 4^113, 5^71, 6^57, 7^48, 8^39, 9^36, 10^34, 11^35, 12^43, 13^35, 14^29, 15^30, 16^24, 17^26, 18^23, 19^13, 20^14, 21^13, 22^18, 23^11, 24^16, 25^15, 26^5, 27^6, 28^8, 29^6, 30^4, 31^8, 32^2, 34^3, 35^5, 37^2, 38^2, 39, 41^2, 42^2, 43, 44, 45, 46, 47, 48^3, 50^2, 51, 53, 54, 57, 62, 64^2, 66^3, 76, 121, 3549]
surviving nonsolo ucounts = 1[3549]
ids = [184]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.33 = TCCCGATTCTTGCCGT-1

using 101 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[2, 98]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.36 = TCCCGATTCTTTAGTC-1

using 126 reads

====================================================================================

graph has 138 edges initially, 4 edges after simplification

total ucounts = 15
nonsolo ucounts = 12[2^3, 4, 5, 6, 7^2, 14, 20, 25, 29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.40 = TCGAGGCAGACATAAC-1

using 607 reads

====================================================================================

graph has 758 edges initially, 16 edges after simplification

total ucounts = 136
nonsolo ucounts = 87[2^22, 3^11, 4^7, 5^7, 6^8, 7^7, 8^5, 9^4, 10^2, 11^3, 12^2, 13, 14^2, 15, 16, 21^2, 22, 32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.45 = TCGAGGCAGAGACTAT-1

using 307 reads

====================================================================================

graph has 252 edges initially, 10 edges after simplification

total ucounts = 13
nonsolo ucounts = 11[2, 5^2, 6, 26^2, 30, 31, 41, 48, 85]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.50 = TCGAGGCAGATAGGAG-1

using 15 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.54 = TCGAGGCAGATGCGAC-1

using 57 reads

====================================================================================

graph has 72 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 7[2^2, 3, 4, 5, 6, 35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.57 = TCGAGGCAGCATCATC-1

using 1470 reads

====================================================================================

graph has 592 edges initially, 4 edges after simplification

total ucounts = 11
nonsolo ucounts = 7[2, 3, 4, 5, 14, 19, 1419]
surviving nonsolo ucounts = 1[1419]
ids = [10]

====================================================================================

REJECT CONTIGS

TIG 1[bases=472]
5-55 ==> 9886-9936 on segment before TRAV40 exon 1 [len=9936] (mis=0)
55-372 ==> 0-317 on |511|TRAV40|L-REGION+V-REGION| [len=317] (mis=3)
369-430 ==> 0-61 on |427|TRAJ40|J-REGION| [len=61] (mis=3)
430-472 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
umis assigned: [10]
of which 1 are surviving nonsolos
reads assigned: 1401
start codons at 25, 55, 91, 166, 212, 259
confident = false
frameshifted full length stopped transcript of length 472
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.66 = TCGAGGCAGGGAGTAA-1

using 30 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.76 = TCGAGGCCAAACTGTC-1

using 3403 reads

====================================================================================

graph has 4397 edges initially, 60 edges after simplification

total ucounts = 1044
nonsolo ucounts = 612[2^216, 3^120, 4^108, 5^43, 6^30, 7^19, 8^18, 9^8, 10^7, 11^5, 12^3, 13^4, 14^3, 15^4, 16^4, 18^4, 21^2, 22^4, 24, 25, 33, 35, 36, 37, 41, 51, 64, 76]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.78 = TCGAGGCCAAAGGTGC-1

using 22 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.80 = TCGAGGCCAAATCCGT-1

using 33 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.88 = TCGAGGCCAAGTTCTG-1

using 33 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.91 = TCGAGGCCACAACGCC-1

using 29 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.92 = TCGAGGCCACAAGACG-1

using 32 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.95 = TCGAGGCCACGCTTTC-1

using 1390 reads

====================================================================================

graph has 1164 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[1390]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.99 = TCGAGGCCACTGTGTA-1

using 12401 reads

====================================================================================

graph has 5133 edges initially, 66 edges after simplification

total ucounts = 443
nonsolo ucounts = 291[2^83, 3^54, 4^37, 5^29, 6^18, 7^14, 8^9, 9^7, 10^3, 11^4, 12^5, 13^4, 14^3, 15, 17^3, 19, 21^2, 23, 25, 27, 35, 36, 38, 51, 425, 610, 762, 812, 1034, 2394, 4659]
surviving nonsolo ucounts = 7[425, 610, 762, 812, 1034, 2394, 4659]
ids = [113, 255, 316, 45, 38, 203, 27]

====================================================================================

UMI info for barcode TCGAGGCCACTGTGTA-1 contig 1 = AAGCCCTGAG...
umi ACCGGCTCGC = 1043 reads: +397 validated
umi CACGGAACGC = 430 reads: +397 validated
umi GCATTCTCAA = 606 reads: +397 validated
umi TAACCATGCT = 761 reads: +397 validated

GOOD CONTIGS

TIG 1[bases=491]
0-42 ==> 267-309 on |630|TRBV5-1|5'UTR| [len=309] (mis=0)
42-385 ==> 0-343 on |631|TRBV5-1|L-REGION+V-REGION| [len=343] (mis=3)
391-439 ==> 0-48 on |548|TRBJ2-5|J-REGION| [len=48] (mis=3)
439-491 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 4 umis using 175 reads
cdr3 = CASSQEGAEETQYF at 369, score = 9 + 7
umis assigned: [38, 113, 255, 316]
of which 4 are surviving nonsolos
reads assigned: 2791
start codons at 42, 324, 328
confident = true

REJECT CONTIGS

TIG 1[bases=497]
4-86 ==> 31-113 on segment before TRBV21-1 exon 2 [len=113] (mis=0)
404-445 ==> 9-50 on |543|TRBJ2-1|J-REGION| [len=50] (mis=0)
445-497 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
umis assigned: [27, 45]
of which 2 are surviving nonsolos
reads assigned: 5242
start codons at 154, 185, 245, 278, 297
confident = false
did not find CDR3

TIG 2[bases=393]
4-289 ==> 282-567 on segment before TRAJ38 exon 1 [len=567] (mis=1)
289-351 ==> 0-62 on |424|TRAJ38|J-REGION| [len=62] (mis=1)
351-393 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
umis assigned: [203]
of which 1 are surviving nonsolos
reads assigned: 2352
start codons at 43, 93, 133, 280, 291
confident = false
did not find CDR3
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.101 = TCGAGGCCAGACGCTC-1

using 47 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[47]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.102 = TCGAGGCCAGATGGGT-1

using 54 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[2^2, 49]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.104 = TCGAGGCCAGCCTGTG-1

using 63 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 4[2, 3, 4, 51]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.105 = TCGAGGCCAGCGATCC-1

using 550 reads

====================================================================================

graph has 460 edges initially, 12 edges after simplification

total ucounts = 38
nonsolo ucounts = 34[2^4, 3^4, 5^3, 6^2, 7^2, 8, 9^2, 13, 16^2, 18, 22^2, 25, 27, 28, 29, 31, 34^2, 42, 48, 54]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.115 = TCGAGGCCATCCGTGG-1

using 58 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[58]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.120 = TCGAGGCCATTACGAC-1

using 62 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[62]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.123 = TCGAGGCGTAAGAGAG-1

using 5385 reads

====================================================================================

graph has 2350 edges initially, 44 edges after simplification

total ucounts = 338
nonsolo ucounts = 209[2^51, 3^37, 4^38, 5^17, 6^13, 7^6, 8^3, 9^7, 10^4, 11^4, 12^4, 13^2, 14, 15, 16^2, 17, 18^2, 19, 22, 23^2, 27, 29, 37, 42, 45^2, 48^2, 64, 180, 366, 3309]
surviving nonsolo ucounts = 4[64, 180, 366, 3309]
ids = [175, 50, 163, 214]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.124 = TCGAGGCGTAAGTGGC-1

using 2131 reads

====================================================================================

graph has 2094 edges initially, 54 edges after simplification

total ucounts = 392
nonsolo ucounts = 242[2^64, 3^51, 4^33, 5^13, 6^12, 7^7, 8^8, 9^7, 10^6, 11^3, 12^6, 13^2, 14^4, 15, 16, 17^2, 19^3, 20^2, 22^2, 27, 28, 31, 37, 38, 40, 41, 42, 45, 58, 59, 62, 79, 84, 131]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.126 = TCGAGGCGTAGCGTCC-1

using 134 reads

====================================================================================

graph has 112 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[134]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.134 = TCGAGGCGTCGACTGC-1

using 25 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.136 = TCGAGGCGTCTAAACC-1

using 82 reads

====================================================================================

graph has 58 edges initially, 2 edges after simplification

total ucounts = 14
nonsolo ucounts = 8[2, 3^2, 5, 8^2, 10, 37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.138 = TCGAGGCGTGATAAGT-1

using 55 reads

====================================================================================

graph has 58 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[55]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.145 = TCGAGGCGTGTCTGAT-1

using 48 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[47]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.146 = TCGAGGCGTGTGACGA-1

using 53 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[53]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.149 = TCGAGGCGTTATCACG-1

using 153 reads

====================================================================================

graph has 152 edges initially, 2 edges after simplification

total ucounts = 26
nonsolo ucounts = 16[2^4, 3, 4, 5, 6^2, 10^2, 12, 17, 20, 21^2]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.151 = TCGAGGCGTTCGCTAA-1

using 6337 reads

====================================================================================

graph has 3162 edges initially, 64 edges after simplification

total ucounts = 316
nonsolo ucounts = 212[2^59, 3^29, 4^26, 5^22, 6^12, 7^3, 8^8, 9^7, 10, 11^2, 12^5, 13^4, 14^4, 15, 16, 17^2, 20^2, 21^2, 23^2, 25, 28, 29, 42, 43, 47, 58, 60, 63, 66, 72, 76, 153, 347, 426, 655, 671, 699, 765, 876]
surviving nonsolo ucounts = 9[58, 153, 347, 426, 655, 671, 699, 765, 876]
ids = [69, 108, 257, 34, 314, 24, 191, 271, 61]

====================================================================================

UMI info for barcode TCGAGGCGTTCGCTAA-1 contig 1 = GAGATGCCTC...
umi ACAGATGCTA = 1 reads: -389 +1 -7X +1 -5X invalidated
umi ACAGGGTCGT = 682 reads: +403 validated
umi AGTTCAACCA = 882 reads: +403 validated
umi CCCTATTGCT = 155 reads: +403 validated
umi TTTTGATATC = 652 reads: +403 validated

UMI info for barcode TCGAGGCGTTCGCTAA-1 contig 2 = ACAGGACCAG...
umi ACGTCTGCAC = 430 reads: +391 validated
umi GGCGCTAGGT = 539 reads: +372 -2 +17 non-validated

GOOD CONTIGS

TIG 1[bases=556]
111-451 ==> 0-340 on |514|TRAV5|L-REGION+V-REGION| [len=340] (mis=3)
448-514 ==> 0-66 on |402|TRAJ18|J-REGION| [len=66] (mis=2)
514-556 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 4 umis using 107 reads
cdr3 = CAEKDRGSTLGRLYF at 438, score = 7 + 8
umis assigned: [22, 24, 61, 108, 314]
of which 4 are surviving nonsolos
reads assigned: 2327
start codons at 3, 111, 165, 181, 330, 336
confident = true

TIG 2[bases=496]
0-53 ==> 21-74 on |591|TRBV2|5'UTR| [len=74] (mis=0)
53-400 ==> 0-347 on |592|TRBV2|L-REGION+V-REGION| [len=347] (mis=4)
397-444 ==> 0-47 on |550|TRBJ2-7|J-REGION| [len=47] (mis=2)
444-496 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 1 umis using 32 reads
cdr3 = CASSIDYEQYF at 383, score = 6 + 8
umis assigned: [34, 191]
of which 2 are surviving nonsolos
reads assigned: 952
start codons at 10, 53, 70, 152, 264, 294, 321, 374
confident = true

REJECT CONTIGS

TIG 1[bases=478]
0-24 ==> 0-24 on |558|TRBV10-3|5'UTR| [len=24] (mis=0)
24-368 ==> 0-344 on |559|TRBV10-3|L-REGION+V-REGION| [len=344] (mis=2)
376-426 ==> 0-50 on |547|TRBJ2-4|J-REGION| [len=50] (mis=2)
426-478 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
umis assigned: [69, 271]
of which 2 are surviving nonsolos
reads assigned: 806
start codons at 0, 24, 46, 78, 82, 146, 174, 202, 229, 265
confident = false
did not find CDR3
now this is a cell
paired!

ACTGGGGACTCAGCTATCTACTTCTGTGCAGAGAAGGACAGAGGCTCAACCCTGGGGAGGCTATACTTTGGAAGAGGAACTCAGTTGACTGTCTGGCCTG <==> ATCCGGTCCACAAAGCTGGAGGACTCAGCCATGTACTTCTGTGCCAGCAGTATCGACTACGAGCAGTACTTCGGGCCGGGCACCAGGCTCACGGTCACAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.155 = TCGAGGCTCAACACGT-1

using 5654 reads

====================================================================================

graph has 2158 edges initially, 52 edges after simplification

total ucounts = 348
nonsolo ucounts = 222[2^49, 3^29, 4^32, 5^17, 6^24, 7^15, 8^7, 9^7, 10^6, 11^6, 12^5, 13^2, 14^4, 15^3, 18, 19, 20, 25, 26, 32, 41^2, 42^2, 49, 59, 60, 66, 1257, 2652]
surviving nonsolo ucounts = 2[1257, 2652]
ids = [277, 5]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.156 = TCGAGGCTCAACCATG-1

using 30 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 2[3, 24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.157 = TCGAGGCTCAACGGGA-1

using 26 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.161 = TCGAGGCTCACCCGAG-1

using 54 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[54]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.163 = TCGAGGCTCAGAGCTT-1

using 84 reads

====================================================================================

graph has 82 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 5[2, 3, 15, 23, 39]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.165 = TCGAGGCTCAGCAACT-1

using 17 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[17]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.172 = TCGAGGCTCATTCACT-1

using 180 reads

====================================================================================

graph has 199 edges initially, 6 edges after simplification

total ucounts = 14
nonsolo ucounts = 13[2^2, 3, 4^3, 8^2, 16, 21, 25, 29, 53]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.175 = TCGAGGCTCCCAAGAT-1

using 21 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.176 = TCGAGGCTCCTAGTGA-1

using 27 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.178 = TCGAGGCTCCTTGCCA-1

using 36 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.179 = TCGAGGCTCGAGAACG-1

using 31 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.185 = TCGAGGCTCGTACCGG-1

using 82 reads

====================================================================================

graph has 68 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 6[2^2, 3, 4, 13, 56]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.186 = TCGAGGCTCGTCACGG-1

using 26 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.190 = TCGAGGCTCTACCTGC-1

using 47 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[47]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.191 = TCGAGGCTCTAGCACA-1

using 24 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.197 = TCGAGGCTCTGGCGTG-1

using 29 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.199 = TCGAGGCTCTGGTTCC-1

using 21 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.205 = TCGAGGCTCTTATCTG-1

using 35 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[2, 33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.207 = TCGCGAGAGACAGGCT-1

using 29 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.220 = TCGCGAGAGGAGCGTT-1

using 2567 reads

====================================================================================

graph has 998 edges initially, 18 edges after simplification

total ucounts = 118
nonsolo ucounts = 64[2^16, 3^8, 4^8, 5^7, 6, 7^3, 8^3, 9, 10, 11, 13, 15, 16, 18, 20, 24, 28, 29, 30, 34, 35, 37, 59, 181, 1770]
surviving nonsolo ucounts = 2[181, 1770]
ids = [34, 56]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.225 = TCGCGAGAGTACTTGC-1

using 50 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[50]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.229 = TCGCGAGAGTTGCAGG-1

using 62 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[4, 58]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.230 = TCGCGAGAGTTTCCTT-1

using 885 reads

====================================================================================

graph has 364 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 1[882]
surviving nonsolo ucounts = 1[882]
ids = [2]

====================================================================================

UMI info for barcode TCGCGAGAGTTTCCTT-1 contig 1 = GGATTCAGTA...
umi GTCGAATATG = 882 reads: +397 validated

GOOD CONTIGS

TIG 1[bases=486]
47-384 ==> 0-337 on |500|TRAV34|L-REGION+V-REGION| [len=337] (mis=5)
385-444 ==> 7-66 on |432|TRAJ45|J-REGION| [len=66] (mis=0)
444-486 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 1 umis using 44 reads
cdr3 = CGPGRGGGADGLTF at 371, score = 7 + 8
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 868
start codons at 47, 195, 222, 245, 248, 279, 303, 354
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.237 = TCGCGAGCAATGCCAT-1

using 146 reads

====================================================================================

graph has 88 edges initially, 6 edges after simplification

total ucounts = 7
nonsolo ucounts = 6[4, 8, 12, 30, 41, 50]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.245 = TCGCGAGCACCTCGTT-1

using 1075 reads

====================================================================================

graph has 1279 edges initially, 20 edges after simplification

total ucounts = 282
nonsolo ucounts = 145[2^53, 3^25, 4^14, 5^10, 6^5, 7^7, 8^6, 9^5, 10^2, 11, 12, 13^2, 14, 15, 17, 21^2, 22^2, 23, 26, 37^2, 38, 47, 70]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.247 = TCGCGAGCACGACTCG-1

using 76 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[34, 42]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.250 = TCGCGAGCACTGTCGG-1

using 27 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.258 = TCGCGAGCATAACCTG-1

using 37 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.261 = TCGCGAGCATCGGAAG-1

using 29 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.263 = TCGCGAGCATGAACCT-1

using 11 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 1[9]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.265 = TCGCGAGCATGTCGAT-1

using 25 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.270 = TCGCGAGCATTTCACT-1

using 21 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.272 = TCGCGAGCATTTGCCC-1

using 387 reads

====================================================================================

graph has 361 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[387]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.275 = TCGCGAGGTAACGTTC-1

using 16 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[6, 9]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.283 = TCGCGAGGTCCTCTTG-1

using 22 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 2[2, 16]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.294 = TCGCGAGGTTACGTCA-1

using 10760 reads

====================================================================================

graph has 4211 edges initially, 108 edges after simplification

total ucounts = 524
nonsolo ucounts = 338[2^109, 3^58, 4^40, 5^19, 6^22, 7^15, 8^15, 9^6, 10^5, 11^4, 12^4, 13^4, 14, 15, 16^4, 17, 19, 21, 22, 23, 25, 27^2, 29, 32^2, 33^2, 39^2, 42^2, 47, 57, 89, 103, 196, 301, 342, 424, 467, 478, 533, 821, 1724, 3145]
surviving nonsolo ucounts = 12[57, 89, 103, 196, 301, 342, 424, 467, 478, 533, 1724, 3145]
ids = [310, 339, 213, 454, 270, 200, 260, 439, 79, 510, ...]

====================================================================================

UMI info for barcode TCGCGAGGTTACGTCA-1 contig 1 = TTTTGAAACC...
umi CGATCAACAG = 100 reads: +287 -96 +17 non-validated
umi CTCTTGTTCG = 423 reads: +400 validated
umi CTTCGCCAAT = 282 reads: -378X +5 -1XX +2 -2XX +1 -1XX +2 -4XX +2 -2XX invalidated
umi GCCGACCATG = 49 reads: +293 -27 +3 -1 +7 -1 +1 -1X +42 -1X +23 invalidated
umi TCTATTCACA = 354 reads: -375X +5 -1XX +1 -3XX +1 -1XX +1 -2XX +2 -1XX +1 -2XX +2 -1XX +1 invalidated
umi TCTCACTGGC = 463 reads: +400 validated
umi TGCTGTCCGC = 1722 reads: -233 +167 non-validated
umi TTGTCGCCCG = 516 reads: +400 validated

UMI info for barcode TCGCGAGGTTACGTCA-1 contig 2 = AGCCCCAAGC...
umi AGCGCTGTAG = 405 reads: -381X +1 -3X +5 -1XX +1 -1XX +2 -2XX +1 -1XX +1 invalidated
umi CCTCCTTTTC = 342 reads: +400 validated
umi GACCACGCCA = 2781 reads: -378 +2 -1XX +1 -3XX +5 -1XX +1 -1XX +2 -2XX +1 -1XX +1 invalidated
umi GTATCAAAGA = 73 reads: -388 +1 -1X +2 -1XX +2 -2XX +1 -1XX +1 invalidated
umi TGACTGTCCT = 172 reads: -336 +64 non-validated

GOOD CONTIGS

TIG 1[bases=570]
0-128 ==> 0-128 on |523|TRAV8-4|5'UTR| [len=128] (mis=0)
128-469 ==> 0-341 on |524|TRAV8-4|L-REGION+V-REGION| [len=341] (mis=0)
468-528 ==> 0-60 on |397|TRAJ12|J-REGION| [len=60] (mis=2)
528-570 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 3 umis using 120 reads
cdr3 = CAVSEVDSSYKLIF at 455, score = 7 + 9
umis assigned: [213, 260, 270, 310, 437, 439, 469, 510]
of which 7 are surviving nonsolos
reads assigned: 3860
start codons at 128, 291, 431
confident = true

TIG 2[bases=476]
0-24 ==> 23-47 on |692|TRBV9|5'UTR| [len=47] (mis=0)
24-367 ==> 0-343 on |693|TRBV9|L-REGION+V-REGION| [len=343] (mis=2)
362-374 ==> 0-12 on |535|TRBD1|D-REGION| [len=12] (mis=3)
374-424 ==> 0-50 on |538|TRBJ1-3|J-REGION| [len=50] (mis=0)
424-476 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 1 umis using 19 reads
cdr3 = CASSAQGSSGNTIYF at 351, score = 9 + 7
umis assigned: [79, 200, 290, 339, 454]
of which 5 are surviving nonsolos
reads assigned: 3768
start codons at 24, 146, 232
confident = true
now this is a cell
paired!

CATATGAGCGACGCGGCTGAGTACTTCTGTGCTGTGAGTGAGGTGGATAGCAGCTATAAATTGATCTTCGGGAGTGGGACCAGACTGCTGGTCAGGCCTG <==> GAGCTGGGGGACTCAGCTTTGTATTTCTGTGCCAGCAGCGCACAGGGGTCCTCTGGAAACACCATATATTTTGGAGAGGGAAGTTGGCTCACTGTTGTAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.303 = TCGCGAGTCAAGAAGT-1

using 56 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[56]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.304 = TCGCGAGTCAATCACG-1

using 48 reads

====================================================================================

graph has 56 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[48]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.305 = TCGCGAGTCACATAGC-1

using 51 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[51]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.306 = TCGCGAGTCACCACCT-1

using 3602 reads

====================================================================================

graph has 4095 edges initially, 106 edges after simplification

total ucounts = 931
nonsolo ucounts = 519[2^184, 3^107, 4^48, 5^51, 6^30, 7^14, 8^11, 9^5, 10^6, 11^7, 12^4, 13^3, 14^4, 15^4, 16, 17^4, 18^3, 19, 20^3, 21, 23^2, 25, 26^2, 27^2, 29, 30^3, 31, 32, 34, 35, 37^2, 39, 41, 43^2, 48, 52^2, 54, 55, 62, 74]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.307 = TCGCGAGTCAGCTTAG-1

using 25 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.314 = TCGCGAGTCATGGTCA-1

using 52 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[52]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.315 = TCGCGAGTCATGTCCC-1

using 35 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 1[33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.321 = TCGCGAGTCCCTAACC-1

using 30 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.324 = TCGCGAGTCGAGAGCA-1

using 5121 reads

====================================================================================

graph has 3334 edges initially, 76 edges after simplification

total ucounts = 498
nonsolo ucounts = 275[2^96, 3^45, 4^28, 5^19, 6^11, 7^6, 8^5, 9^8, 10^5, 11^4, 12^4, 14^2, 15, 16^3, 18^4, 20^2, 21^3, 22, 25^3, 26^4, 27, 28, 29, 30, 31, 32, 33, 34, 38^2, 39^2, 41^3, 44, 50, 55, 60, 833, 1972]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================

REJECT CONTIGS

TIG 1[bases=412]
3-306 ==> 632-935 on segment before TRAJ10 exon 1 [len=935] (mis=1)
306-370 ==> 0-64 on |395|TRAJ10|J-REGION| [len=64] (mis=0)
370-412 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
umis assigned: [59, 224]
of which 0 are surviving nonsolos
reads assigned: 2727
start codons at 26, 54, 107, 124, 157, 161
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.325 = TCGCGAGTCGATAGAA-1

using 894 reads

====================================================================================

graph has 330 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 3[6^2, 876]
surviving nonsolo ucounts = 1[876]
ids = [7]

====================================================================================

UMI info for barcode TCGCGAGTCGATAGAA-1 contig 1 = CCCATCCTGC...
umi TGCGTTTTCT = 883 reads: +409 validated

GOOD CONTIGS

TIG 1[bases=483]
0-22 ==> 17-39 on |672|TRBV7-2|5'UTR| [len=39] (mis=0)
22-369 ==> 0-347 on |673|TRBV7-2|L-REGION+V-REGION| [len=347] (mis=0)
383-431 ==> 0-48 on |548|TRBJ2-5|J-REGION| [len=48] (mis=1)
431-483 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 1 umis using 78 reads
cdr3 = CASSLAQLEIFQETQYF at 352, score = 8 + 7
umis assigned: [7]
of which 1 are surviving nonsolos
reads assigned: 865
start codons at 22, 131
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.333 = TCGCGAGTCTCCAACC-1

using 147 reads

====================================================================================

graph has 130 edges initially, 4 edges after simplification

total ucounts = 15
nonsolo ucounts = 12[2^3, 4, 5, 8^2, 11, 15, 25, 30, 32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.334 = TCGCGAGTCTCCGGTT-1

using 52 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[52]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.335 = TCGCGAGTCTCGATGA-1

using 9198 reads

====================================================================================

graph has 4319 edges initially, 90 edges after simplification

total ucounts = 412
nonsolo ucounts = 248[2^88, 3^45, 4^24, 5^18, 6^8, 7^15, 8^3, 9^4, 10^6, 11^3, 12^2, 13^3, 14^2, 15, 16, 17^2, 18, 19, 22, 25, 27, 31, 32, 33, 42, 53, 54, 57, 59, 94, 302, 706, 779, 811, 819, 824, 921, 1022, 1323]
surviving nonsolo ucounts = 7[302, 706, 811, 819, 824, 921, 1022]
ids = [400, 271, 39, 132, 407, 195, 245]

====================================================================================

UMI info for barcode TCGCGAGTCTCGATGA-1 contig 1 = AGAGGCCCCA...
umi AGTTTTTGCC = 78 reads: -387X +3 -1XX +1 -2XX +2 -2XX +2 invalidated
umi CCCCATCGGC = 820 reads: +400 validated
umi CTCCACTGGT = 934 reads: +400 validated
umi GCAGGCCAGT = 1031 reads: +400 validated
umi GGCTTCTCAG = 713 reads: +400 validated
umi TTGTAAACAG = 298 reads: +400 validated
umi TTTGGTACTA = 830 reads: +400 validated

GOOD CONTIGS

TIG 1[bases=481]
0-29 ==> 82-111 on |628|TRBV4-3|5'UTR| [len=111] (mis=0)
29-373 ==> 0-344 on |629|TRBV4-3|L-REGION+V-REGION| [len=344] (mis=1)
382-429 ==> 0-47 on |550|TRBJ2-7|J-REGION| [len=47] (mis=5)
429-481 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 6 umis using 295 reads
cdr3 = CASSQGTQTGEEQYF at 356, score = 10 + 8
umis assigned: [57, 132, 195, 245, 271, 400, 407]
of which 6 are surviving nonsolos
reads assigned: 4611
start codons at 29, 83, 122, 128, 151, 179, 221, 286
confident = true

REJECT CONTIGS

TIG 1[bases=444]
0-56 ==> 0-56 on |529|TRAV9-2|5'UTR| [len=56] (mis=1)
56-394 ==> 0-338 on |530|TRAV9-2|L-REGION+V-REGION| [len=338] (mis=5)
387-444 ==> 0-57 on |396|TRAJ11|J-REGION| [len=60] (mis=3)
cdr3 = CALGSGYSTLTF at 380, score = 9 + 8
umis assigned: [39, 253, 354]
of which 1 are surviving nonsolos
reads assigned: 1529
start codons at 15, 56, 131, 219, 273, 428
confident = false
VJ delta = 23
not full
not full
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.337 = TCGCGAGTCTTACCGC-1

using 36 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.339 = TCGCGAGTCTTGAGGT-1

using 566 reads

====================================================================================

graph has 232 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 1[563]
surviving nonsolo ucounts = 1[563]
ids = [0]

====================================================================================

UMI info for barcode TCGCGAGTCTTGAGGT-1 contig 1 = ATTATTTTTT...
umi CGCGTTGCCT = 540 reads: +406 validated

GOOD CONTIGS

TIG 1[bases=504]
0-56 ==> 0-56 on |461|TRAV12-3|5'UTR| [len=56] (mis=0)
56-399 ==> 0-343 on |462|TRAV12-3|L-REGION+V-REGION| [len=343] (mis=0)
402-462 ==> 0-60 on |437|TRAJ5|J-REGION| [len=60] (mis=1)
462-504 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 1 umis using 17 reads
cdr3 = CAMSGADTGRRALTF at 386, score = 9 + 9
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 535
start codons at 56, 59, 221, 263, 294, 392
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.343 = TCGCGTTAGAAGCCCA-1

using 167 reads

====================================================================================

graph has 178 edges initially, 4 edges after simplification

total ucounts = 12
nonsolo ucounts = 10[3, 5^2, 10, 11, 12, 17, 21, 36, 45]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.344 = TCGCGTTAGACAATAC-1

using 37 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.345 = TCGCGTTAGACACTAA-1

using 440 reads

====================================================================================

graph has 391 edges initially, 8 edges after simplification

total ucounts = 25
nonsolo ucounts = 21[2^2, 3, 7^3, 9, 10^2, 11^2, 13, 17, 18, 27, 32, 38, 39, 52, 55, 66]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.347 = TCGCGTTAGACGCACA-1

using 290 reads

====================================================================================

graph has 206 edges initially, 12 edges after simplification

total ucounts = 23
nonsolo ucounts = 18[2, 4, 5^2, 6, 8^2, 10, 13, 15, 16, 18, 20^2, 23, 27, 35, 50]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.350 = TCGCGTTAGAGTGAGA-1

using 256 reads

====================================================================================

graph has 269 edges initially, 16 edges after simplification

total ucounts = 21
nonsolo ucounts = 15[2^2, 3, 5^3, 7, 8, 16, 25^2, 27^2, 32, 61]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.352 = TCGCGTTAGATGCGAC-1

using 11853 reads

====================================================================================

graph has 9470 edges initially, 326 edges after simplification

total ucounts = 974
nonsolo ucounts = 733[2^109, 3^90, 4^67, 5^50, 6^22, 7^24, 8^14, 9^17, 10^14, 11^9, 12^8, 13^15, 14^15, 15^7, 16^6, 17^13, 18^9, 19^16, 20^4, 21^12, 22^12, 23^8, 24^8, 25^8, 26^3, 27^11, 28^5, 29^2, 30^11, 31^11, 32^5, 33^7, 34^6, 35^8, 36^3, 37^9, 38^4, 39^5, 40^11, 41^4, 42^2, 43^3, 44^6, 45^7, 46^6, 47^3, 48^7, 49^2, 50^2, 51^4, 52^4, 53^3, 54, 55, 56^3, 57, 58^2, 59^2, 61^2, 62^2, 64, 66, 68, 70, 74, 79, 87, 94]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.353 = TCGCGTTAGCACCGCT-1

using 154 reads

====================================================================================

graph has 148 edges initially, 8 edges after simplification

total ucounts = 11
nonsolo ucounts = 10[2, 3^2, 7, 8, 15, 18, 23, 29, 45]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.354 = TCGCGTTAGCAGATCG-1

using 49 reads

====================================================================================

graph has 29 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[48]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.355 = TCGCGTTAGCCAACAG-1

using 101 reads

====================================================================================

graph has 122 edges initially, 4 edges after simplification

total ucounts = 13
nonsolo ucounts = 9[3, 4, 6, 7, 8^2, 9, 16, 36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.356 = TCGCGTTAGCCACGTC-1

using 28 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 4[2^3, 19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.357 = TCGCGTTAGCGATATA-1

using 29 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.358 = TCGCGTTAGCGCTTAT-1

using 219 reads

====================================================================================

graph has 356 edges initially, 10 edges after simplification

total ucounts = 9
nonsolo ucounts = 8[5^2, 9, 12, 14, 51, 59, 63]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.362 = TCGCGTTAGGCATGGT-1

using 220 reads

====================================================================================

graph has 178 edges initially, 8 edges after simplification

total ucounts = 18
nonsolo ucounts = 14[2^5, 8, 9, 11, 19, 21, 26, 33, 39, 40]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.363 = TCGCGTTAGGCGACAT-1

using 629 reads

====================================================================================

graph has 619 edges initially, 46 edges after simplification

total ucounts = 42
nonsolo ucounts = 37[2^3, 3^4, 5^3, 6^2, 7, 9, 12^2, 14, 15^3, 18, 19, 21, 22^2, 24, 25^2, 26, 28, 31^2, 32, 34^2, 37, 51]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.365 = TCGCGTTAGGGTCTCC-1

using 138 reads

====================================================================================

graph has 136 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 7[5, 6, 12, 20, 25, 33, 36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.366 = TCGCGTTAGGTGCAAC-1

using 202 reads

====================================================================================

graph has 302 edges initially, 10 edges after simplification

total ucounts = 13
nonsolo ucounts = 10[5, 6^2, 9, 20, 21, 29, 32^2, 39]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.367 = TCGCGTTAGTACATGA-1

using 151 reads

====================================================================================

graph has 142 edges initially, 14 edges after simplification

total ucounts = 20
nonsolo ucounts = 16[2^4, 4^3, 7^3, 8, 11, 12, 14, 18, 43]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.368 = TCGCGTTAGTACGATA-1

using 59 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[59]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.369 = TCGCGTTAGTACGTTC-1

using 56 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[56]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.372 = TCGCGTTAGTCGCCGT-1

using 94 reads

====================================================================================

graph has 102 edges initially, 4 edges after simplification

total ucounts = 9
nonsolo ucounts = 5[2, 16, 20^2, 32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.373 = TCGCGTTAGTGCCAGA-1

using 97 reads

====================================================================================

graph has 78 edges initially, 2 edges after simplification

total ucounts = 17
nonsolo ucounts = 12[2^2, 3^2, 4, 5, 9^2, 10, 14, 15, 16]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.379 = TCGCGTTCAACACCTA-1

using 23 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.384 = TCGCGTTCAAGTTCTG-1

using 197 reads

====================================================================================

graph has 126 edges initially, 12 edges after simplification

total ucounts = 13
nonsolo ucounts = 13[2^3, 4^2, 6, 7, 23, 26, 27^2, 30, 37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.398 = TCGCGTTCACGAAACG-1

using 23 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 1[21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.405 = TCGCGTTCAGCCACCA-1

using 36 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 5[2, 3, 4, 5, 18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.408 = TCGCGTTCAGCCTTGG-1

using 37 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 5[2^2, 3, 5, 24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.409 = TCGCGTTCAGCGAACA-1

using 16023 reads

====================================================================================

graph has 9717 edges initially, 171 edges after simplification

total ucounts = 1217
nonsolo ucounts = 772[2^233, 3^153, 4^109, 5^52, 6^36, 7^27, 8^21, 9^12, 10^8, 11^16, 12^4, 13^3, 14^3, 15^3, 17^2, 18^5, 19^3, 20^2, 21^5, 22^4, 24^2, 25^4, 26^4, 27, 29^2, 30^3, 31, 32, 33, 35, 36, 37, 39, 40, 41, 42^2, 43, 44, 47, 48, 49^3, 50, 52^2, 54^2, 59, 64^2, 66, 68, 70, 75, 78, 79^2, 81^2, 92, 97, 103, 131, 149, 276, 288, 352, 389, 441, 516, 573, 582, 584, 597, 724, 851, 856, 960, 1581]
surviving nonsolo ucounts = 18[79, 103, 131, 149, 276, 352, 389, 441, 516, 573, 582, 584, 597, 724, 851, 856, 960, 1581]
ids = [701, 978, 1083, 100, 882, 1084, 38, 49, 499, 923, ...]

====================================================================================

UMI info for barcode TCGCGTTCAGCGAACA-1 contig 1 = GCCAGGTTCA...
umi AACGGCATGT = 442 reads: +409 validated
umi ACAAGTTTTA = 583 reads: +409 validated
umi ACAGATGGTC = 152 reads: +363 -29 +17 non-validated
umi CGCATAGGGC = 525 reads: +409 validated
umi CTTAAGACGC = 737 reads: +409 validated
umi GCACCAGTCG = 1464 reads: -378 +14 -1XX +3 -1XX +1 -1XX +3 -1XX +1 -1XX +3 -1XX invalidated
umi TATGAATGGC = 594 reads: +57 -1XX +351 invalidated
umi TGTGATGTCC = 353 reads: +409 validated

UMI info for barcode TCGCGTTCAGCGAACA-1 contig 2 = GGAGGCAGTG...
umi AACAGAAGGG = 360 reads: -379 +2 -1XX +1 -2XX +1 -3XX +2 invalidated
umi ACCATAGATC = 601 reads: +391 validated
umi ATTGTTCTCT = 965 reads: +391 validated
umi CCATAGCGCT = 587 reads: +391 validated
umi CTCGCTCTTT = 855 reads: +391 validated
umi GCGATTGCAG = 79 reads: -10X +381 invalidated
umi TAGACAGCCT = 252 reads: -379 +2 -1XX +1 -2XX +1 -3XX +2 invalidated
umi TCGCCGGGCT = 91 reads: +391 validated
umi TGATCGGTTA = 258 reads: -376X +1 -4X +1 -2XX +1 -2XX +1 -1XX +2 invalidated
umi TGGCATAGCT = 854 reads: +391 validated
umi TGTGATGGAT = 113 reads: -382X +1 -2XX +1 -3XX +2 invalidated

GOOD CONTIGS

TIG 1[bases=526]
0-75 ==> 0-75 on |467|TRAV14/DV4|5'UTR| [len=75] (mis=3)
75-425 ==> 0-350 on |468|TRAV14/DV4|L-REGION+V-REGION| [len=350] (mis=7)
421-484 ==> 0-63 on |426|TRAJ4|J-REGION| [len=63] (mis=2)
484-526 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 6 umis using 179 reads
cdr3 = CAMRQFSGGYNKLIF at 408, score = 7 + 9
umis assigned: [49, 91, 100, 499, 600, 677, 923, 1084]
of which 8 are surviving nonsolos
reads assigned: 4740
start codons at 75, 165, 208, 229, 267, 295, 307, 399, 414
confident = true

TIG 2[bases=498]
0-55 ==> 23-78 on |595|TRBV20-1|5'UTR| [len=78] (mis=0)
55-382 ==> 0-327 on |596|TRBV20-1|L-REGION+V-REGION| [len=335] (mis=0)
399-446 ==> 6-53 on |541|TRBJ1-6|J-REGION| [len=53] (mis=0)
446-498 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 7 umis using 264 reads
cdr3 = CSIHTGEGNSPLHF at 376, score = 7 + 9
umis assigned: [38, 115, 305, 409, 571, 701, 882, 978, 1027, 1061] and 1 others
of which 10 are surviving nonsolos
reads assigned: 4920
start codons at 55, 193, 229, 235, 248, 308
confident = true
now this is a cell
paired!

CTGGGGGACTCAGCAATGTATTTCTGTGCAATGAGACAATTTTCTGGTGGCTACAATAAGCTGATTTTTGGAGCAGGGACCAGGCTGGCTGTACACCCAT <==> GCCCATCCTGAAGACAGCAGCTTCTACATCTGCAGTATTCATACAGGGGAGGGTAATTCACCCCTCCACTTTGGGAACGGGACCAGGCTCACTGTGACAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.410 = TCGCGTTCAGCTGCAC-1

using 61 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[61]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.411 = TCGCGTTCAGGGATTG-1

using 47 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[47]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.412 = TCGCGTTCAGGTCTCG-1

using 25 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.413 = TCGCGTTCATACGCCG-1

using 67 reads

====================================================================================

graph has 54 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 3[4, 30, 33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.419 = TCGCGTTCATTTGCCC-1

using 34 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.421 = TCGCGTTGTAATCACC-1

using 41 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[10, 31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.422 = TCGCGTTGTACATCCA-1

using 4515 reads

====================================================================================

graph has 1936 edges initially, 32 edges after simplification

total ucounts = 320
nonsolo ucounts = 190[2^67, 3^37, 4^24, 5^14, 6^8, 7^9, 8^5, 9^5, 10, 11^5, 12, 13, 14, 20^2, 22, 31, 41, 47, 49, 51, 54, 56, 59, 3223]
surviving nonsolo ucounts = 1[3223]
ids = [74]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.423 = TCGCGTTGTACCCAAT-1

using 58 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[58]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.426 = TCGCGTTGTATCGCAT-1

using 261 reads

====================================================================================

graph has 56 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[5, 256]
surviving nonsolo ucounts = 1[256]
ids = [1]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.431 = TCGCGTTGTCAGTGGA-1

using 27 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 1[25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.432 = TCGCGTTGTCATCCCT-1

using 230 reads

====================================================================================

graph has 120 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[229]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.434 = TCGCGTTGTCGCGGTT-1

using 43 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[43]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.440 = TCGCGTTGTGAAAGAG-1

using 19 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.455 = TCGCGTTGTTGTACAC-1

using 15063 reads

====================================================================================

graph has 4098 edges initially, 28 edges after simplification

total ucounts = 225
nonsolo ucounts = 127[2^38, 3^19, 4^19, 5^11, 6^10, 7^2, 8^2, 9^4, 10^2, 13, 19, 20, 27, 43, 88, 103, 311, 612, 652, 653, 698, 745, 847, 946, 1059, 1352, 1658, 1927, 2782]
surviving nonsolo ucounts = 13[311, 612, 652, 653, 698, 745, 847, 946, 1059, 1352, 1658, 1927, 2782]
ids = [121, 68, 143, 59, 37, 51, 167, 208, 165, 12, ...]

====================================================================================

UMI info for barcode TCGCGTTGTTGTACAC-1 contig 1 = CCTTTTCACC...
umi AATATATCTC = 1341 reads: -24X +1 -3XX +1 -3XX +2 -4XX +1 -1XX +1 -1XX +1 -2XX +343 invalidated
umi ACATCGCGGT = 1656 reads: -42 +4 -1XX +341 invalidated
umi AGCGATCATT = 692 reads: +388 validated
umi ATATTCGCTT = 748 reads: +388 validated
umi ATTGATGAGT = 649 reads: +388 validated
umi CACTAACCGG = 612 reads: +388 validated
umi CGTATTTCAT = 2767 reads: -152X +1 -1XX +234 invalidated
umi GGTAGCGTTA = 1949 reads: -43X +3 -1XX +129 -8XX +4 -6XX +1 -5XX +1 -110X +1 -7XX +2 -3XX +1 -10XX +53 invalidated
umi GTCCTATCGT = 75 reads: -378 +2 -1XX +3 -2XX +2 invalidated
umi TACACTGTAG = 1054 reads: +388 validated
umi TACCGCGGGC = 842 reads: +388 validated
umi TTACCTATAT = 945 reads: +388 validated

UMI info for barcode TCGCGTTGTTGTACAC-1 contig 2 = CTCTGGAAAT...
umi GCAGACATAT = 320 reads: +391 validated
umi GGGCCTTAGG = 655 reads: +391 validated

GOOD CONTIGS

TIG 1[bases=493]
0-53 ==> 27-80 on |651|TRBV6-2|5'UTR| [len=80] (mis=0)
53-397 ==> 0-344 on |652|TRBV6-2|L-REGION+V-REGION| [len=344] (mis=3)
391-441 ==> 0-50 on |543|TRBJ2-1|J-REGION| [len=50] (mis=2)
441-493 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 10 umis using 889 reads
cdr3 = CASRFYNEQFF at 380, score = 9 + 8
umis assigned: [12, 18, 37, 51, 59, 68, 98, 148, 154, 165] and 2 others
of which 11 are surviving nonsolos
reads assigned: 13094
start codons at 11, 53, 111, 164, 188, 195, 203, 230, 294, 303, 399
confident = true

TIG 2[bases=510]
0-77 ==> 132-209 on |489|TRAV26-1|5'UTR| [len=209] (mis=0)
77-405 ==> 0-328 on |490|TRAV26-1|L-REGION+V-REGION| [len=328] (mis=1)
414-468 ==> 7-61 on |427|TRAJ40|J-REGION| [len=61] (mis=0)
468-510 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 2 umis using 52 reads
cdr3 = CIVRVPAQPGTYKYIF at 389, score = 8 + 8
umis assigned: [121, 143]
of which 2 are surviving nonsolos
reads assigned: 951
start codons at 77, 129, 155, 216, 222, 273, 288, 297, 302
confident = true
now this is a cell
paired!

AGAGACACTGCTGTGTACTATTGCATCGTCAGAGTCCCGGCGCAACCAGGAACCTACAAATACATCTTTGGAACAGGCACCAGGCTGAAGGTTTTAGCAA <==> TTGGAGTCGGCTGCTCCCTCCCAAACATCTGTGTACTTCTGTGCCAGCAGGTTCTACAATGAGCAGTTCTTCGGGCCAGGGACACGGCTCACCGTGCTAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.460 = TCGCGTTTCACCCGAG-1

using 48 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[48]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.462 = TCGCGTTTCACGCATA-1

using 56 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[56]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.465 = TCGCGTTTCAGAAATG-1

using 42 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 5[2, 3^2, 4, 25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.468 = TCGCGTTTCAGGCGAA-1

using 29 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.470 = TCGCGTTTCATGTCTT-1

using 64 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[63]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.476 = TCGCGTTTCCCGACTT-1

using 32 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.481 = TCGCGTTTCCGCAGTG-1

using 38 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.484 = TCGCGTTTCCTCAACC-1

using 30 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.485 = TCGCGTTTCCTCCTAG-1

using 43 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 5[2, 3, 4, 10, 21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.488 = TCGCGTTTCGATCCCT-1

using 30 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.489 = TCGCGTTTCGCTAGCG-1

using 1023 reads

====================================================================================

graph has 658 edges initially, 14 edges after simplification

total ucounts = 51
nonsolo ucounts = 47[2^5, 3^4, 4^3, 6^3, 7^3, 8^2, 10^2, 12, 15, 18^2, 20^2, 21, 22, 23, 27, 29, 33, 38^2, 39, 41^2, 43, 46, 47, 48, 55, 61, 69, 86]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.496 = TCGCGTTTCTTCCTTC-1

using 9522 reads

====================================================================================

graph has 6865 edges initially, 134 edges after simplification

total ucounts = 1382
nonsolo ucounts = 905[2^241, 3^146, 4^120, 5^89, 6^71, 7^53, 8^40, 9^29, 10^27, 11^15, 12^20, 13^9, 14^2, 15^5, 16^2, 17^3, 18^3, 20^3, 21^3, 23^2, 24, 25, 26^2, 28, 33, 34, 36, 39, 40, 45, 48, 49, 50^2, 56, 61, 64, 80, 89, 93, 3625]
surviving nonsolo ucounts = 2[93, 3625]
ids = [193, 636]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.497 = TCGCGTTTCTTGCATT-1

using 57 reads

====================================================================================

graph has 52 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[2, 4, 14, 35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.501 = TCGGGACAGACCGGAT-1

using 41 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[40]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.504 = TCGGGACAGATCCCGC-1

using 217 reads

====================================================================================

graph has 224 edges initially, 6 edges after simplification

total ucounts = 48
nonsolo ucounts = 16[2^3, 3, 4^3, 5^2, 6^2, 10, 12, 20, 22, 78]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.506 = TCGGGACAGCCACGCT-1

using 29 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.509 = TCGGGACAGCTGAAAT-1

using 26 reads

====================================================================================

graph has 6 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.511 = TCGGGACAGGATCGCA-1

using 60 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[60]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.513 = TCGGGACAGGCATGGT-1

using 93 reads

====================================================================================

graph has 108 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[93]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.515 = TCGGGACAGGCTAGAC-1

using 35 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.517 = TCGGGACAGGGCTTCC-1

using 16 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.521 = TCGGGACAGTTACCCA-1

using 727 reads

====================================================================================

graph has 802 edges initially, 18 edges after simplification

total ucounts = 146
nonsolo ucounts = 89[2^27, 3^11, 4^8, 5^7, 6^7, 7^2, 8^5, 9^5, 10^2, 11, 12^2, 13, 14, 16, 19, 20, 22^3, 28, 31, 35, 78]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.525 = TCGGGACCAAAGTCAA-1

using 101 reads

====================================================================================

graph has 112 edges initially, 2 edges after simplification

total ucounts = 12
nonsolo ucounts = 10[2^2, 3, 4, 5, 7, 8, 11^2, 46]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.526 = TCGGGACCAAGACGTG-1

using 18 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.527 = TCGGGACCAAGGTTTC-1

using 35 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.528 = TCGGGACCAATAGAGT-1

using 80 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[79]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.529 = TCGGGACCAATCTACG-1

using 6203 reads

====================================================================================

graph has 3791 edges initially, 68 edges after simplification

total ucounts = 537
nonsolo ucounts = 367[2^78, 3^59, 4^41, 5^41, 6^30, 7^15, 8^24, 9^15, 10^8, 11^6, 12^3, 13^3, 14^3, 15^4, 16^3, 17, 19, 20^4, 21^2, 22, 25^3, 26, 27, 28, 29, 32, 33, 34^2, 35, 37^2, 41, 51, 52, 56, 62, 63^2, 66, 67, 84, 3136]
surviving nonsolo ucounts = 1[3136]
ids = [106]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.542 = TCGGGACCAGGTTTCA-1

using 80 reads

====================================================================================

graph has 112 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 4[2, 5, 13, 59]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.554 = TCGGGACGTAAAGTCA-1

using 32 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.555 = TCGGGACGTAAATACG-1

using 100 reads

====================================================================================

graph has 64 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[12, 43, 44]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.556 = TCGGGACGTAAGAGGA-1

using 46 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[46]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.567 = TCGGGACGTCAGAATA-1

using 47 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[47]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.569 = TCGGGACGTCCCGACA-1

using 31 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.570 = TCGGGACGTCCGAGTC-1

using 55768 reads

====================================================================================

graph has 12571 edges initially, 88 edges after simplification

total ucounts = 342
nonsolo ucounts = 185[2^45, 3^20, 4^10, 5^11, 6^7, 7^8, 8^6, 9^4, 10^2, 11^4, 12^3, 13^2, 16, 18^2, 19, 27, 28^2, 36, 37^2, 41, 47, 59, 85, 92, 249, 337, 390, 476, 543, 592, 606, 621, 643, 660, 669, 670, 678, 723, 755, 764^2, 767, 773, 783, 792, 799, 808, 829, 839, 850, 857, 860, 886, 897, 906, 931, 952, 1049, 1074, 1129, 1181, 1196, 1474, 1554, 1945, 1987, 2344, 2365, 2722, 3234, 3347, 4200]
surviving nonsolo ucounts = 47[249, 337, 476, 543, 592, 606, 621, 643, 660, 669, 670, 678, 723, 755, 764^2, 767, 773, 783, 792, 799, 808, 829, 839, 850, 857, 860, 886, 897, 906, 931, 952, 1049, 1074, 1129, 1181, 1196, 1474, 1554, 1945, 1987, 2344, 2365, 2722, 3234, 3347, 4200]
ids = [164, 238, 57, 128, 167, 51, 136, 79, 11, 40, ...]

====================================================================================

UMI info for barcode TCGGGACGTCCGAGTC-1 contig 1 = ATCAATGCAC...
umi AAAGATATCT = 847 reads: +397 validated
umi AACACTCTTT = 832 reads: +397 validated
umi AAGATAAGGA = 655 reads: +397 validated
umi AAGGAAAGCA = 1842 reads: -360 +1 -1X +1 -3XX +6 -1XX +1 -1XX +4 -1XX +6 -1XX +2 -1XX +2 -4XX +1 invalidated
umi AGCACTATGT = 764 reads: +397 validated
umi AGTCTTTCGG = 598 reads: +397 validated
umi ATATTTCTCT = 793 reads: +397 validated
umi ATCAATATGC = 1855 reads: -363 +2 -1XX +2 -1XX +2 -1XX +2 -1XX +6 -1XX +4 -1XX +2 -1XX +2 -4XX +1 invalidated
umi ATGGTTCACG = 1997 reads: -49X +1 -3XX +344 invalidated
umi ATGTTTGTGT = 779 reads: +397 validated
umi ATTCGAACGC = 3474 reads: -360 +5 -1XX +2 -1XX +2 -1XX +2 -1XX +6 -1XX +4 -1XX +2 -1XX +2 -4XX +1 invalidated
umi CAATTCTGGG = 1950 reads: -44X +2 -3XX +348 invalidated
umi CGATTTCGCT = 794 reads: +397 validated
umi CGCGGAACCG = 540 reads: +382 -8 +7 non-validated
umi CGTTATACCA = 1531 reads: +397 validated
umi CTAAGATCAT = 619 reads: +397 validated
umi GACCTAATCA = 586 reads: +397 validated
umi GCGCGGAATT = 858 reads: +397 validated
umi GCTCCTGCCT = 540 reads: +23 -1 +5 -3XX +1 -2XX +1 -2XX +1 -1XX +1 -1XX +1 -2XX +352 invalidated
umi GTAAAAGGGT = 1485 reads: -73X +324 invalidated
umi GTCCAATCCA = 863 reads: +397 validated
umi GTCGTAGATA = 789 reads: +397 validated
umi GTTACGGAGT = 743 reads: +397 validated
umi TAATGTCTCA = 1090 reads: +397 validated
umi TAGCACAGGC = 1194 reads: +397 validated
umi TAGGTGCCTC = 1137 reads: -27X +1 -4XX +1 -2XX +1 -2XX +1 -1XX +1 -1XX +1 -2XX +352 invalidated
umi TATGAACCGC = 761 reads: +397 validated
umi TCTTTGGGTT = 816 reads: +397 validated
umi TGTCCGGGGC = 876 reads: -360 +5 -1XX +2 -1XX +2 -1XX +2 -1XX +6 -1XX +4 -1XX +2 -1XX +2 -4XX +1 invalidated
umi TTGGTTATTT = 1200 reads: +88 -4XX +1 -1XX +2 -2XX +1 -1XX +1 -4XX +1 -1XX +2 -33XX +2 -7XX +1 -6XX +1 -3XX +1 -1XX +1 -1XX +2 -2XX +227 invalidated
umi TTTCTTGGTG = 949 reads: +397 validated

UMI info for barcode TCGGGACGTCCGAGTC-1 contig 2 = AGCCCAGAGA...
umi AAATAACAGC = 767 reads: +394 validated
umi ACAATGGGCT = 928 reads: +394 validated
umi ACCTAGTTGG = 937 reads: +394 validated
umi ACGTCTAATG = 891 reads: +394 validated
umi AGAAAAGGAA = 674 reads: +394 validated
umi ATAGTTCGCT = 473 reads: +394 validated
umi ATTCAGCAGC = 643 reads: +394 validated
umi CACTCTGGAA = 675 reads: +394 validated
umi GCCATACAGC = 812 reads: +394 validated
umi GTTAGTAGGC = 341 reads: +394 validated
umi TCCCGTTAGA = 727 reads: +394 validated
umi TGTAGAAGTA = 904 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=495]
0-46 ==> 0-46 on |661|TRBV6-6|5'UTR| [len=46] (mis=0)
46-382 ==> 0-336 on |662|TRBV6-6|L-REGION+V-REGION| [len=344] (mis=2)
396-443 ==> 0-47 on |550|TRBJ2-7|J-REGION| [len=47] (mis=3)
443-495 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 25 umis using 1588 reads
cdr3 = CASDRGGGDYEQYF at 373, score = 9 + 8
umis assigned: [1, 4, 11, 13, 42, 51, 63, 64, 73, 76] and 21 others
of which 31 are surviving nonsolos
reads assigned: 33196
start codons at 4, 46, 104, 157, 181, 196, 223, 287
confident = true

TIG 2[bases=521]
0-85 ==> 12-97 on |492|TRAV27|5'UTR| [len=97] (mis=0)
85-413 ==> 0-328 on |493|TRAV27|L-REGION+V-REGION| [len=328] (mis=2)
413-479 ==> 0-66 on |402|TRAJ18|J-REGION| [len=66] (mis=1)
479-521 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 12 umis using 469 reads
cdr3 = CAGADRGSTLGRLYF at 403, score = 10 + 8
umis assigned: [2, 22, 29, 30, 40, 57, 79, 101, 176, 238] and 2 others
of which 12 are surviving nonsolos
reads assigned: 8595
start codons at 58, 67, 85, 129, 237, 338
confident = true

REJECT CONTIGS

TIG 1[bases=303]
4-199 ==> 5805-6000 on segment before TRBD1 exon 1 [len=6000] (mis=0)
203-251 ==> 0-48 on |537|TRBJ1-2|J-REGION| [len=48] (mis=1)
251-303 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
umis assigned: [164, 215, 335]
of which 3 are surviving nonsolos
reads assigned: 6608
start codons at 76, 86, 160, 209
confident = false
did not find CDR3

TIG 2[bases=322]
5-114 ==> 5719-5828 on segment before TRBD1 exon 1 [len=6000] (mis=0)
219-270 ==> 0-51 on |539|TRBJ1-4|J-REGION| [len=51] (mis=0)
270-322 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
umis assigned: [200]
of which 1 are surviving nonsolos
reads assigned: 2675
start codons at 211, 225
confident = false
did not find CDR3
now this is a cell
paired!

CCTGGTGATACAGGCCTCTACCTCTGTGCAGGAGCGGACAGAGGCTCAACCCTGGGGAGGCTATACTTTGGAAGAGGAACTCAGTTGACTGTCTGGCCTG <==> GCTGCTCCCTCCCAGACATCTGTGTACTTCTGTGCCAGCGACCGTGGGGGAGGGGACTACGAGCAGTACTTCGGGCCGGGCACCAGGCTCACGGTCACAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.578 = TCGGGACGTTAAAGAC-1

using 76 reads

====================================================================================

graph has 68 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 5[3^2, 4, 16, 48]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.579 = TCGGGACGTTACGGAG-1

using 591 reads

====================================================================================

graph has 116 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[591]
surviving nonsolo ucounts = 1[591]
ids = [0]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.581 = TCGGGACGTTATGTGC-1

using 23 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[8, 15]
surviving nonsolo ucounts = 1[15]
ids = [1]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.590 = TCGGGACTCACATACG-1

using 177 reads

====================================================================================

graph has 142 edges initially, 2 edges after simplification

total ucounts = 14
nonsolo ucounts = 11[2^3, 8^2, 10, 11, 14, 21, 34, 62]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.592 = TCGGGACTCACGATGT-1

using 9872 reads

====================================================================================

graph has 3982 edges initially, 26 edges after simplification

total ucounts = 315
nonsolo ucounts = 218[2^52, 3^43, 4^31, 5^13, 6^15, 7^16, 8^5, 9^5, 10^6, 11^4, 12^2, 13^5, 14^3, 15, 17, 19^2, 20, 21, 25, 33, 34^2, 40, 50, 198, 605, 1030, 1167, 2623, 2881]
surviving nonsolo ucounts = 6[198, 605, 1030, 1167, 2623, 2881]
ids = [8, 191, 311, 127, 101, 257]

====================================================================================

UMI info for barcode TCGGGACTCACGATGT-1 contig 1 = GATCTTAATT...
umi GCTGCAGTTT = 594 reads: +394 validated

UMI info for barcode TCGGGACTCACGATGT-1 contig 2 = TGGGGCTTCA...
umi AACCCAGTAC = 192 reads: -383X +1 -3XX +3 -5XX +1 -5XX +2 invalidated
umi CCAGGTCGAC = 2638 reads: +403 validated
umi CGCTATTTTA = 1186 reads: +403 validated
umi TCCCTTTCGA = 1788 reads: -338X +2 -2XX +2 -6XX +2 -1XX +1 -6XX +43 invalidated
umi TTTGTTCTAG = 1034 reads: +403 validated

GOOD CONTIGS

TIG 1[bases=459]
0-23 ==> 0-23 on |463|TRAV13-1|5'UTR| [len=23] (mis=0)
23-360 ==> 0-337 on |464|TRAV13-1|L-REGION+V-REGION| [len=337] (mis=5)
362-417 ==> 8-63 on |398|TRAJ13|J-REGION| [len=63] (mis=0)
417-459 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 1 umis using 35 reads
cdr3 = CAAVHGGYQKVTF at 347, score = 9 + 8
umis assigned: [191]
of which 1 are surviving nonsolos
reads assigned: 582
start codons at 23, 78, 87, 237
confident = true

TIG 2[bases=473]
18-362 ==> 0-344 on |613|TRBV28|L-REGION+V-REGION| [len=344] (mis=3)
359-375 ==> 0-16 on |730|TRBD2|D-REGION| [len=16] (mis=2)
374-421 ==> 0-47 on |550|TRBJ2-7|J-REGION| [len=47] (mis=5)
421-473 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 3 umis using 314 reads
cdr3 = CASSYGLAGGGDEQYF at 345, score = 6 + 8
umis assigned: [8, 101, 127, 257, 311]
of which 5 are surviving nonsolos
reads assigned: 6703
start codons at 18, 76, 140, 153, 160, 168, 223, 226, 234, 336
confident = true
now this is a cell
paired!

ACCCAACCTGAAGACTCGGCTGTCTACTTCTGTGCAGCGGTTCACGGGGGTTACCAGAAAGTTACCTTTGGAACTGGAACAAAGCTCCAAGTCATCCCAA <==> ACCAACCAGACATCTATGTACCTCTGTGCCAGCAGTTACGGACTAGCGGGAGGAGGGGACGAGCAGTACTTCGGGCCGGGCACCAGGCTCACGGTCACAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.595 = TCGGGACTCAGGATCT-1

using 38 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.598 = TCGGGACTCCAAACTG-1

using 44 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[44]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.600 = TCGGGACTCCACGAAT-1

using 25 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.610 = TCGGGACTCGGCATCG-1

using 25 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[4, 21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.611 = TCGGGACTCGTCACGG-1

using 62 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[4, 5, 51]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.612 = TCGGGACTCGTCTGAA-1

using 150 reads

====================================================================================

graph has 116 edges initially, 6 edges after simplification

total ucounts = 12
nonsolo ucounts = 8[3, 11, 12, 16, 18, 23, 24, 39]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.613 = TCGGGACTCGTGGACC-1

using 22 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.614 = TCGGGACTCTATCGCC-1

using 80 reads

====================================================================================

graph has 84 edges initially, 6 edges after simplification

total ucounts = 13
nonsolo ucounts = 9[2, 3^2, 5^2, 9, 13, 17, 19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.615 = TCGGGACTCTATGTGG-1

using 65 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[65]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.624 = TCGGTAAAGAGCTGGT-1

using 5558 reads

====================================================================================

graph has 3777 edges initially, 90 edges after simplification

total ucounts = 411
nonsolo ucounts = 320[2^73, 3^44, 4^28, 5^22, 6^21, 7^21, 8^10, 9^14, 10^4, 11^6, 12^10, 13^8, 14^6, 15^3, 16^5, 17^4, 18, 19, 20^3, 21, 22^3, 24^3, 25^2, 26, 30^2, 32, 34, 38, 39^2, 41, 48^2, 51, 54, 62, 68, 69, 72, 83, 87, 138, 253, 280, 324, 334, 354, 380, 561]
surviving nonsolo ucounts = 9[25, 138, 253, 280, 324, 334, 354, 380, 561]
ids = [285, 82, 179, 213, 275, 1, 25, 341, 76]

====================================================================================

UMI info for barcode TCGGTAAAGAGCTGGT-1 contig 1 = AGATCAGAAG...
umi AATGTTCAGA = 330 reads: -393X +3 -4XX +1 -1XX +2 -1XX +2 -1XX +1 -1XX +3 -2XX invalidated
umi AGGTATCTAT = 565 reads: +415 validated
umi CGTTCTCATT = 234 reads: -400X +1 -1XX +2 -1XX +2 -1XX +1 -1XX +3 -2XX invalidated
umi TCGTATCGCT = 377 reads: +415 validated

GOOD CONTIGS

TIG 1[bases=503]
0-46 ==> 0-46 on |506|TRAV38-2/DV8|5'UTR| [len=46] (mis=0)
46-395 ==> 0-349 on |507|TRAV38-2/DV8|L-REGION+V-REGION| [len=349] (mis=0)
398-461 ==> 0-63 on |444|TRAJ58|J-REGION| [len=63] (mis=5)
461-503 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 2 umis using 50 reads
cdr3 = CAYRSARGETSGSRLTF at 379, score = 7 + 9
umis assigned: [25, 76, 179, 341]
of which 4 are surviving nonsolos
reads assigned: 1484
start codons at 46, 51, 103, 136, 179, 238, 278, 362, 370
confident = true
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.625 = TCGGTAAAGAGGGATA-1

using 91 reads

====================================================================================

graph has 104 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 7[3, 4, 5, 8, 10, 11, 48]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.629 = TCGGTAAAGATCCTGT-1

using 28 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.633 = TCGGTAAAGCAGGTCA-1

using 8329 reads

====================================================================================

graph has 3916 edges initially, 62 edges after simplification

total ucounts = 301
nonsolo ucounts = 213[2^33, 3^28, 4^24, 5^21, 6^12, 7^9, 8^9, 9^15, 10^5, 11^3, 12^2, 13^6, 14^4, 15^2, 16^3, 18^4, 19^2, 20^6, 21, 23, 25, 28, 30, 31^2, 32^2, 35, 43, 47, 51, 52, 53, 62, 249, 421, 432, 477, 659, 726, 912, 957, 1570]
surviving nonsolo ucounts = 10[47, 249, 421, 432, 477, 659, 726, 912, 957, 1570]
ids = [267, 155, 206, 109, 275, 159, 225, 57, 126, 222]

====================================================================================

UMI info for barcode TCGGTAAAGCAGGTCA-1 contig 1 = AATGCACAGA...
umi CATTTAACCA = 422 reads: +388 validated
umi CGAGTCTACG = 954 reads: +388 validated
umi CTTTTCGGGT = 229 reads: -24X +1 -3XX +1 -3XX +2 -4XX +1 -1XX +1 -1XX +1 -2XX +343 invalidated
umi TAATCGTGGC = 726 reads: +388 validated
umi TGCTAAGTTG = 41 reads: -380 +8 non-validated
umi TGTTCTTTTA = 474 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=483]
0-43 ==> 5-48 on |649|TRBV6-2|5'UTR| [len=48] (mis=0)
43-387 ==> 0-344 on |650|TRBV6-2|L-REGION+V-REGION| [len=344] (mis=2)
383-431 ==> 0-48 on |548|TRBJ2-5|J-REGION| [len=48] (mis=2)
431-483 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 5 umis using 210 reads
cdr3 = CASSYLETQYF at 370, score = 9 + 7
umis assigned: [109, 126, 155, 225, 267, 275]
of which 6 are surviving nonsolos
reads assigned: 2789
start codons at 1, 43, 101, 154, 178, 185, 193, 220, 284, 293
confident = true

REJECT CONTIGS

TIG 1[bases=463]
0-23 ==> 0-23 on |463|TRAV13-1|5'UTR| [len=23] (mis=0)
23-360 ==> 0-337 on |464|TRAV13-1|L-REGION+V-REGION| [len=337] (mis=6)
359-421 ==> 0-62 on |415|TRAJ3|J-REGION| [len=62] (mis=1)
421-463 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
umis assigned: [57, 159]
of which 2 are surviving nonsolos
reads assigned: 1505
start codons at 23, 78, 87, 237
confident = false
frameshifted full length transcript of length 463
did not find CDR3

TIG 2[bases=439]
4-336 ==> 10-342 on |733|TRBV11-2|L-REGION+V-REGION| [len=342] (mis=4)
333-345 ==> 0-12 on |535|TRBD1|D-REGION| [len=12] (mis=3)
348-387 ==> 9-48 on |537|TRBJ1-2|J-REGION| [len=48] (mis=0)
387-439 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
cdr3 = CQLYRGPCYTF at 326, score = 3 + 9
umis assigned: [222]
of which 1 are surviving nonsolos
reads assigned: 1549
start codons at 136, 217
confident = false
not full
VJ delta = 10
not full
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.645 = TCGGTAAAGCTCAACT-1

using 74 reads

====================================================================================

graph has 64 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 6[2, 4^2, 5, 8, 50]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.646 = TCGGTAAAGCTGCAAG-1

using 58 reads

====================================================================================

graph has 48 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[58]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.648 = TCGGTAAAGGCATGTG-1

using 18105 reads

====================================================================================

graph has 8454 edges initially, 124 edges after simplification

total ucounts = 762
nonsolo ucounts = 555[2^103, 3^69, 4^52, 5^57, 6^42, 7^36, 8^17, 9^22, 10^27, 11^12, 12^13, 13^8, 14^3, 15^9, 16^6, 17^5, 18^8, 19^3, 20^3, 21^3, 22^3, 24, 25^3, 26^5, 28^2, 30^3, 32, 33^3, 35, 39, 44^2, 47, 48^2, 49, 51^3, 56, 60^2, 62, 67, 75, 78, 80^3, 363, 405, 493, 499, 629, 819, 971, 986, 1011, 1022, 1038, 1106, 1134, 1268, 1289]
surviving nonsolo ucounts = 16[78, 363, 405, 493, 499, 629, 819, 971, 986, 1011, 1022, 1038, 1106, 1134, 1268, 1289]
ids = [607, 171, 574, 515, 194, 369, 565, 750, 615, 342, ...]

====================================================================================

UMI info for barcode TCGGTAAAGGCATGTG-1 contig 1 = AGGAGGACCA...
umi AGGGAGCTCT = 1296 reads: +391 validated
umi ATGATCTTCC = 350 reads: +389 -1XX +1 invalidated
umi ATTTTCTTGT = 501 reads: +391 validated
umi CAGCGCCCTT = 1275 reads: +391 validated
umi CGGTCTTATA = 1019 reads: +391 validated
umi CTGTTGTCGT = 1109 reads: +391 validated
umi GTGGACCTGG = 1029 reads: +391 validated
umi TAAACGCGGA = 821 reads: +391 validated
umi TAGCACGTAT = 1045 reads: +391 validated
umi TCAACTTTAG = 987 reads: +391 validated

UMI info for barcode TCGGTAAAGGCATGTG-1 contig 2 = GCCAGGTTCA...
umi CTATGCGCAC = 627 reads: +409 validated
umi GGTCACTTCC = 490 reads: +409 validated
umi TAATCGCTTA = 329 reads: +385 -5 +19 non-validated

GOOD CONTIGS

TIG 1[bases=494]
0-51 ==> 258-309 on |630|TRBV5-1|5'UTR| [len=309] (mis=0)
51-394 ==> 0-343 on |631|TRBV5-1|L-REGION+V-REGION| [len=343] (mis=1)
401-442 ==> 8-49 on |546|TRBJ2-3|J-REGION| [len=49] (mis=0)
442-494 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 10 umis using 556 reads
cdr3 = CASSLTSSTQYF at 378, score = 9 + 8
umis assigned: [126, 171, 194, 228, 342, 394, 547, 565, 591, 615]
of which 10 are surviving nonsolos
reads assigned: 9255
start codons at 51, 333, 337
confident = true

TIG 2[bases=526]
0-75 ==> 0-75 on |467|TRAV14/DV4|5'UTR| [len=75] (mis=3)
75-419 ==> 0-344 on |468|TRAV14/DV4|L-REGION+V-REGION| [len=350] (mis=4)
422-484 ==> 0-62 on |428|TRAJ41|J-REGION| [len=62] (mis=1)
484-526 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 2 umis using 46 reads
cdr3 = CAMSFNSNSGYALNF at 408, score = 7 + 7
umis assigned: [369, 515, 574]
of which 3 are surviving nonsolos
reads assigned: 1416
start codons at 75, 165, 208, 229, 267, 295, 307, 399, 414, 439
confident = true

REJECT CONTIGS

TIG 1[bases=482]
3-25 ==> 9502-9524 on segment before TRBV21-1 exon 1 [len=9524] (mis=0)
380-430 ==> 0-50 on |540|TRBJ1-5|J-REGION| [len=50] (mis=0)
430-482 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
umis assigned: [268, 607]
of which 2 are surviving nonsolos
reads assigned: 1192
start codons at 25, 143, 174, 234, 267, 286, 406
confident = false
did not find CDR3

TIG 2[bases=470]
0-69 ==> 0-69 on |478|TRAV20|5'UTR| [len=69] (mis=0)
69-406 ==> 0-337 on |479|TRAV20|L-REGION+V-REGION| [len=337] (mis=2)
404-455 ==> 9-60 on |411|TRAJ26|J-REGION| [len=60] (mis=0)
cdr3 = CAVQWSEF at 393, score = 8 + 4
umis assigned: [750]
of which 1 are surviving nonsolos
reads assigned: 953
start codons at 69, 78, 404
confident = false
frameshifted full length transcript of length 470
VJ delta = 15
not full
not full
now this is a cell
paired!

CTGGGGGACTCAGCAATGTATTTCTGTGCAATGAGCTTCAACTCAAATTCCGGGTATGCACTCAACTTCGGCAAAGGCACCTCGCTGTTGGTCACACCCC <==> AGCACCTTGGAGCTGGGGGACTCGGCCCTTTATCTTTGCGCCAGCAGCTTGACTAGTTCTACGCAGTATTTTGGCCCAGGCACCCGGCTGACAGTGCTCG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.649 = TCGGTAAAGGCCCGTT-1

using 260 reads

====================================================================================

graph has 212 edges initially, 10 edges after simplification

total ucounts = 19
nonsolo ucounts = 13[2, 4^2, 5, 7, 8, 9, 11, 18, 28^2, 61, 69]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.651 = TCGGTAAAGGGAAACA-1

using 23583 reads

====================================================================================

graph has 10641 edges initially, 190 edges after simplification

total ucounts = 928
nonsolo ucounts = 658[2^99, 3^67, 4^64, 5^40, 6^51, 7^42, 8^37, 9^27, 10^15, 11^25, 12^24, 13^21, 14^18, 15^14, 16^10, 17^8, 18^5, 19^6, 20^6, 21^7, 22^6, 24^2, 25^3, 26^2, 27^5, 28, 29, 30, 31^3, 32^4, 34, 35, 37^2, 39^3, 40^2, 41, 42, 45^2, 48, 49, 52^2, 53, 56, 59, 60^2, 69, 73, 102, 152, 387, 463, 470, 539, 550, 697, 840, 850, 885, 916, 921, 929, 1107, 1116, 1152, 1261, 1352, 2521]
surviving nonsolo ucounts = 19[152, 387, 463, 470, 539, 550, 697, 840, 850, 885, 916, 921, 929, 1107, 1116, 1152, 1261, 1352, 2521]
ids = [775, 327, 128, 668, 495, 585, 595, 810, 324, 122, ...]

====================================================================================

UMI info for barcode TCGGTAAAGGGAAACA-1 contig 1 = GTCATCCCTC...
umi AAAGGGTAGC = 915 reads: +388 validated
umi AACGGAGTAC = 1261 reads: +388 validated
umi AAGGGAGTAT = 1000 reads: -368 +1 -3X +3 -5XX +1 -5XX +2 invalidated
umi ACTATATCAT = 894 reads: +388 validated
umi ACTCTACGGA = 427 reads: -374 +1 -5X +1 -5XX +2 invalidated
umi ATCGTGTTTC = 925 reads: +388 validated
umi CAAATATATG = 1125 reads: +388 validated
umi CATTATTGGA = 862 reads: +388 validated
umi CCAAACGCTC = 385 reads: +388 validated
umi GAAAGTGCTC = 1146 reads: +388 validated
umi GCTTTTACAC = 512 reads: -368X +1 -3X +3 -5XX +1 -5XX +2 invalidated
umi GGCCAGGGGA = 703 reads: +388 validated
umi TACCCACTGT = 1657 reads: -369X +6 -1XX +1 -1XX +2 -1XX +2 -3XX +2 invalidated
umi TGAAATGTCA = 839 reads: +388 validated
umi TTCATGCAAT = 849 reads: -373 +2 -5XX +1 -5XX +2 invalidated

UMI info for barcode TCGGTAAAGGGAAACA-1 contig 2 = TTTTGAAACC...
umi CTTTATTTTA = 524 reads: -12XX +1 -44X +2 -1XX +1 -1XX +1 -5XX +1 -1XX +3 -2XX +1 -3XX +1 -1XX +316 invalidated
umi TAAACTCTTC = 463 reads: +397 validated
umi TCGACTGCTT = 143 reads: +324 -54 +19 non-validated

GOOD CONTIGS

TIG 1[bases=518]
0-78 ==> 0-78 on |597|TRBV20-1|5'UTR| [len=78] (mis=0)
78-413 ==> 0-335 on |598|TRBV20-1|L-REGION+V-REGION| [len=335] (mis=5)
419-466 ==> 0-47 on |550|TRBJ2-7|J-REGION| [len=47] (mis=1)
466-518 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 10 umis using 508 reads
cdr3 = CSAAPRTAYEQYF at 399, score = 7 + 8
umis assigned: [14, 36, 49, 122, 128, 223, 260, 324, 327, 503] and 5 others
of which 15 are surviving nonsolos
reads assigned: 13336
start codons at 21, 78, 216, 252, 258, 271, 331
confident = true

TIG 2[bases=567]
0-128 ==> 0-128 on |523|TRAV8-4|5'UTR| [len=128] (mis=0)
128-469 ==> 0-341 on |524|TRAV8-4|L-REGION+V-REGION| [len=341] (mis=0)
473-525 ==> 8-60 on |396|TRAJ11|J-REGION| [len=60] (mis=0)
525-567 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 2 umis using 48 reads
cdr3 = CAVSEGGYSTLTF at 455, score = 7 + 8
umis assigned: [495, 668, 775]
of which 3 are surviving nonsolos
reads assigned: 1097
start codons at 128, 291, 431, 506
confident = true

REJECT CONTIGS

TIG 1[bases=466]
16-354 ==> 0-338 on |477|TRAV2|L-REGION+V-REGION| [len=338] (mis=2)
360-424 ==> 0-64 on |395|TRAJ10|J-REGION| [len=64] (mis=2)
424-466 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
cdr3 = CCLLLCCGVFSGLTGGGNKLTF at 327, score = 4 + 8
umis assigned: [418]
of which 1 are surviving nonsolos
reads assigned: 1330
start codons at 16, 173, 210, 268, 275, 323
confident = false
frameshifted full length stopped transcript of length 466
VJ delta = 40
delta too large
not full
not full
now this is a cell
paired!

GCCCATATGAGCGACGCGGCTGAGTACTTCTGTGCTGTGAGTGAGGGGGGATACAGCACCCTCACCTTTGGGAAGGGGACTATGCTTCTAGTCTCTCCAG <==> AGTGCCCATCCTGAAGACAGCAGCTTCTACATCTGCAGTGCTGCCCCTAGGACCGCCTACGAGCAGTACTTCGGGCCGGGCACCAGGCTCACGGTCACAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.653 = TCGGTAAAGTATTGGA-1

using 6238 reads

====================================================================================

graph has 2623 edges initially, 64 edges after simplification

total ucounts = 254
nonsolo ucounts = 191[2^40, 3^22, 4^15, 5^11, 6^4, 7^12, 8^12, 9^3, 10^2, 11^7, 12^4, 13^3, 14^2, 15^3, 16^3, 17^4, 18^4, 19^3, 20^8, 21^3, 22, 23^3, 24^3, 25^6, 26, 27, 28^2, 29, 32, 33, 36, 39, 207, 351, 679, 3130]
surviving nonsolo ucounts = 4[207, 351, 679, 3130]
ids = [7, 169, 108, 9]

====================================================================================

UMI info for barcode TCGGTAAAGTATTGGA-1 contig 1 = TTTTGAAACC...
umi ACTCCTTCAC = 183 reads: -384X +2 -4XX +2 -4XX +2 -1XX +1 invalidated
umi CTGTTTGACC = 597 reads: +399 -1XX invalidated

GOOD CONTIGS

TIG 1[bases=570]
0-128 ==> 0-128 on |523|TRAV8-4|5'UTR| [len=128] (mis=0)
128-469 ==> 0-341 on |524|TRAV8-4|L-REGION+V-REGION| [len=341] (mis=4)
473-528 ==> 8-63 on |398|TRAJ13|J-REGION| [len=63] (mis=1)
528-570 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 1 umis using 9 reads
cdr3 = CAVTPGGGYQKVTF at 455, score = 7 + 8
umis assigned: [7, 108]
of which 2 are surviving nonsolos
reads assigned: 770
start codons at 128, 291, 431
confident = true
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.660 = TCGGTAAAGTTACGGG-1

using 912 reads

====================================================================================

graph has 659 edges initially, 24 edges after simplification

total ucounts = 41
nonsolo ucounts = 36[2^3, 4^2, 5, 6, 7^2, 8, 9, 10^2, 11, 15, 19^2, 21, 22, 23^2, 24, 26, 29, 33^2, 35, 39, 44, 48, 51, 55, 58, 65, 66, 72]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.672 = TCGGTAACACTAGTAC-1

using 38 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[14, 22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.674 = TCGGTAACACTGTTAG-1

using 16403 reads

====================================================================================

graph has 6761 edges initially, 112 edges after simplification

total ucounts = 433
nonsolo ucounts = 326[2^37, 3^36, 4^31, 5^26, 6^27, 7^16, 8^8, 9^11, 10^11, 11^8, 12^9, 13^11, 14^13, 15^6, 16^8, 17^11, 18^9, 19^4, 20^2, 21, 22^6, 23^4, 24^2, 26^3, 27, 28, 29, 31, 32^2, 36, 38^2, 45, 48, 51, 53, 60, 65, 76, 387, 492, 892, 1192, 1352, 1386, 1467, 1662, 1728, 2491]
surviving nonsolo ucounts = 10[387, 492, 892, 1192, 1352, 1386, 1467, 1662, 1728, 2491]
ids = [361, 7, 430, 227, 317, 95, 190, 43, 145, 322]

====================================================================================

UMI info for barcode TCGGTAACACTGTTAG-1 contig 1 = GGGATTCAGA...
umi AACTAGGGAT = 506 reads: +397 validated
umi CCACTGGATC = 1740 reads: +397 validated
umi CTGCCGGTTA = 1197 reads: +397 validated

UMI info for barcode TCGGTAACACTGTTAG-1 contig 2 = GGGCTTCAAA...
umi ACTATTTCCG = 1667 reads: +400 validated
umi ATTCCGCTGC = 1248 reads: -379 +4 -1XX +2 -1XX +3 -1XX +2 -1XX +1 -1XX +4 invalidated
umi CGGAGCCACA = 1479 reads: +400 validated
umi TACTCCGGGG = 1354 reads: +400 validated
umi TAGCACCGCG = 2506 reads: +400 validated
umi TCCTCCCGTC = 343 reads: -388 +2 -1XX +2 -1XX +1 -1XX +4 invalidated
umi TTTTGAGCGT = 893 reads: +400 validated

GOOD CONTIGS

TIG 1[bases=478]
39-376 ==> 0-337 on |498|TRAV30|L-REGION+V-REGION| [len=337] (mis=2)
380-436 ==> 10-66 on |429|TRAJ42|J-REGION| [len=66] (mis=0)
436-478 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 3 umis using 195 reads
cdr3 = CGTEMEGSQGNLIF at 363, score = 7 + 10
umis assigned: [7, 145, 227]
of which 3 are surviving nonsolos
reads assigned: 3371
start codons at 39, 151, 214, 237, 271, 295, 375
confident = true

TIG 2[bases=468]
16-360 ==> 0-344 on |613|TRBV28|L-REGION+V-REGION| [len=344] (mis=5)
360-372 ==> 0-12 on |535|TRBD1|D-REGION| [len=12] (mis=1)
366-416 ==> 0-50 on |543|TRBJ2-1|J-REGION| [len=50] (mis=5)
416-468 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 5 umis using 469 reads
cdr3 = CASSAPGQGDNEQFF at 343, score = 6 + 8
umis assigned: [43, 95, 190, 317, 322, 361, 430]
of which 7 are surviving nonsolos
reads assigned: 9357
start codons at 16, 74, 138, 151, 158, 166, 221, 224, 232, 334, 374
confident = true
now this is a cell
paired!

CAGCTCAGTTACTCAGGAACCTACTTCTGTGGCACAGAGATGGAAGGAAGCCAAGGAAATCTCATCTTTGGAAAAGGCACTAAACTCTCTGTTAAACCAA <==> AGCACCAACCAGACATCTATGTACCTCTGTGCCAGCAGTGCCCCGGGACAGGGGGACAATGAGCAGTTCTTCGGGCCAGGGACACGGCTCACCGTGCTAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.675 = TCGGTAACAGACAAAT-1

using 73 reads

====================================================================================

graph has 104 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 5[2, 3, 8, 11, 45]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.682 = TCGGTAACAGCTCGAC-1

using 10283 reads

====================================================================================

graph has 5220 edges initially, 86 edges after simplification

total ucounts = 684
nonsolo ucounts = 444[2^106, 3^76, 4^54, 5^45, 6^30, 7^17, 8^15, 9^13, 10^10, 11^6, 12^5, 13^6, 14^7, 15^7, 16^4, 17^3, 18^3, 19^2, 20, 21^2, 22, 23^3, 25^2, 27^2, 29^2, 30, 36, 38, 40, 42, 43, 44, 45, 47^3, 62, 86, 359, 386, 396, 400, 424, 528, 569, 643, 3285]
surviving nonsolo ucounts = 9[359, 386, 396, 400, 424, 528, 569, 643, 3285]
ids = [44, 572, 248, 442, 396, 216, 115, 49, 514]

====================================================================================

UMI info for barcode TCGGTAACAGCTCGAC-1 contig 1 = AGCTGTGAGG...
umi ACCAGATCAC = 637 reads: +409 validated
umi ATAGGGCCAG = 569 reads: +409 validated
umi CCCACTGGCC = 504 reads: -373X +1 -6X +1 -8XX +1 -4XX +2 -12XX +1 invalidated
umi CGATAATCTC = 403 reads: +409 validated
umi GGAGTGACAG = 426 reads: +409 validated
umi GTGATGCTCG = 372 reads: +409 validated
umi TCCAACCGCC = 2939 reads: -373X +1 -6XX +1 -8XX +1 -4XX +2 -12XX +1 invalidated
umi TGCTTAACGA = 353 reads: -373X +1 -6X +1 -8XX +1 -4XX +2 -12XX +1 invalidated

GOOD CONTIGS

TIG 1[bases=542]
0-81 ==> 0-81 on |583|TRBV18|5'UTR| [len=81] (mis=0)
81-428 ==> 0-347 on |584|TRBV18|L-REGION+V-REGION| [len=347] (mis=2)
441-490 ==> 0-49 on |546|TRBJ2-3|J-REGION| [len=49] (mis=5)
490-542 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 5 umis using 110 reads
cdr3 = CASSPTTSGGSGDTQYF at 411, score = 7 + 8
umis assigned: [49, 115, 216, 248, 396, 442, 514, 572]
of which 8 are surviving nonsolos
reads assigned: 6111
start codons at 66, 81, 139, 150, 203, 213, 229, 273, 304, 315
confident = true

REJECT CONTIGS

TIG 1[bases=515]
0-111 ==> 0-111 on |494|TRAV29/DV5|5'UTR| [len=111] (mis=0)
111-469 ==> 0-358 on |495|TRAV29/DV5|L-REGION+V-REGION| [len=358] (mis=0)
477-515 ==> 16-54 on |436|TRAJ49|J-REGION| [len=56] (mis=0)
cdr3 = CAASGNAFYF at 456, score = 9 + 7
umis assigned: [44]
of which 1 are surviving nonsolos
reads assigned: 270
start codons at 2, 9, 30, 111, 117, 184, 187, 273, 290, 358, 364, 472
confident = false
VJ delta = 18
not full
not full
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.688 = TCGGTAACAGTCAGCC-1

using 45 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[2, 42]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.692 = TCGGTAACATCAGTAC-1

using 75 reads

====================================================================================

graph has 68 edges initially, 4 edges after simplification

total ucounts = 8
nonsolo ucounts = 5[3, 6, 11, 17, 35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.693 = TCGGTAACATCGGAAG-1

using 22 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.695 = TCGGTAACATGCTGGC-1

using 55 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[55]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.697 = TCGGTAACATTAACCG-1

using 637 reads

====================================================================================

graph has 236 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 4[2^2, 4, 629]
surviving nonsolo ucounts = 1[629]
ids = [3]

====================================================================================

UMI info for barcode TCGGTAACATTAACCG-1 contig 1 = CTTCCTCACG...
umi TGTCTCGTGC = 629 reads: +400 validated

GOOD CONTIGS

TIG 1[bases=546]
0-104 ==> 28-132 on |518|TRAV8-1|5'UTR| [len=132] (mis=0)
104-445 ==> 0-341 on |519|TRAV8-1|L-REGION+V-REGION| [len=341] (mis=1)
438-504 ==> 0-66 on |429|TRAJ42|J-REGION| [len=66] (mis=2)
504-546 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 1 umis using 26 reads
cdr3 = CAVNDRGSQGNLIF at 431, score = 8 + 10
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 618
start codons at 104, 134, 153, 226, 240, 267, 441
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.699 = TCGGTAACATTTCACT-1

using 259 reads

====================================================================================

graph has 174 edges initially, 4 edges after simplification

total ucounts = 15
nonsolo ucounts = 12[2^2, 3^2, 5^2, 7, 9, 22^2, 80, 96]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.703 = TCGGTAAGTAGAAAGG-1

using 59 reads

====================================================================================

graph has 56 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[59]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.712 = TCGGTAAGTCGAGATG-1

using 181 reads

====================================================================================

graph has 110 edges initially, 8 edges after simplification

total ucounts = 6
nonsolo ucounts = 5[2, 27, 33, 43, 75]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.714 = TCGGTAAGTGAGTGAC-1

using 31 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.716 = TCGGTAAGTGCAACTT-1

using 36 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.718 = TCGGTAAGTGGTAACG-1

using 35 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.720 = TCGGTAAGTGTCTGAT-1

using 34 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.731 = TCGGTAATCAACGGGA-1

using 386 reads

====================================================================================

graph has 286 edges initially, 16 edges after simplification

total ucounts = 19
nonsolo ucounts = 15[2^2, 4, 5, 6, 7, 10, 11, 14, 32, 47, 51, 58^2, 75]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.739 = TCGGTAATCCAAGTAC-1

using 11199 reads

====================================================================================

graph has 5147 edges initially, 98 edges after simplification

total ucounts = 541
nonsolo ucounts = 391[2^57, 3^43, 4^46, 5^36, 6^24, 7^17, 8^19, 9^15, 10^15, 11^12, 12^13, 13^9, 14^13, 15^14, 16^10, 17^6, 18^4, 19^2, 20^5, 21^4, 22^3, 24, 25^2, 26^2, 28, 33, 37, 39, 41^2, 42^2, 43, 44, 48^2, 49, 53, 54, 61, 1673, 1928, 3893]
surviving nonsolo ucounts = 3[1673, 1928, 3893]
ids = [426, 532, 381]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.740 = TCGGTAATCCACGACG-1

using 11751 reads

====================================================================================

graph has 1930 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 2[3949, 7799]
surviving nonsolo ucounts = 2[3949, 7799]
ids = [4, 2]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.743 = TCGGTAATCCATGAAC-1

using 157 reads

====================================================================================

graph has 168 edges initially, 2 edges after simplification

total ucounts = 20
nonsolo ucounts = 14[2, 3^3, 4, 5, 12, 13, 14, 15^3, 22, 25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.744 = TCGGTAATCCTTGACC-1

using 47 reads

====================================================================================

graph has 52 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[8, 13, 25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.746 = TCGGTAATCGCAGGCT-1

using 21 reads

====================================================================================

graph has 6 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.755 = TCGGTAATCTCATTCA-1

using 60 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[60]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.759 = TCGGTAATCTCGTATT-1

using 54 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[54]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.764 = TCGGTAATCTGCAGTA-1

using 48 reads

====================================================================================

graph has 76 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 4[2, 5, 9, 32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.766 = TCGGTAATCTGTCTAT-1

using 50443 reads

====================================================================================

graph has 16741 edges initially, 232 edges after simplification

total ucounts = 1028
nonsolo ucounts = 790[2^115, 3^88, 4^51, 5^63, 6^37, 7^48, 8^34, 9^32, 10^29, 11^28, 12^23, 13^23, 14^22, 15^18, 16^13, 17^20, 18^17, 19^12, 20^14, 21^10, 22^10, 23^14, 24^3, 25^3, 26, 27^3, 29, 30^3, 31^2, 32^2, 33^3, 36^2, 37, 38^3, 39, 42, 49, 52, 53, 57, 61, 63, 67, 79, 81^2, 85, 92, 97, 318, 384, 517, 583, 654, 724, 729, 762, 799, 829, 1100, 1144, 1149, 1242, 1243, 1246, 1249, 1291, 1298, 1329, 1436, 1457, 1567, 4334, 4767, 5111, 5128]
surviving nonsolo ucounts = 25[318, 583, 654, 724, 729, 762, 799, 829, 1100, 1144, 1149, 1242, 1243, 1246, 1249, 1291, 1298, 1329, 1436, 1457, 1567, 4334, 4767, 5111, 5128]
ids = [613, 882, 694, 766, 565, 904, 424, 0, 93, 604, ...]

====================================================================================

UMI info for barcode TCGGTAATCTGTCTAT-1 contig 1 = TGGGGAGAAG...
umi AAAACAGGGC = 807 reads: +400 validated
umi ACAAGTGGGT = 1468 reads: +400 validated
umi ATTTTTCGCC = 1147 reads: +400 validated
umi CCAATGCCGT = 1245 reads: +400 validated
umi CGTCTGTCAA = 797 reads: +400 validated
umi CTAGGAACTG = 1230 reads: +400 validated
umi GCTTGAACCG = 1159 reads: +400 validated
umi GGAGTTCGCA = 319 reads: +400 validated
umi TCAGTACATC = 1242 reads: +400 validated
umi TCCCACGACA = 1311 reads: +400 validated
umi TGCATCTGTT = 586 reads: +400 validated
umi TGGCTATCGT = 736 reads: +400 validated
umi TTCTGCTCCA = 1288 reads: +386 -2XX +1 -3XX +3 -3XX +1 -1XX invalidated

UMI info for barcode TCGGTAATCTGTCTAT-1 contig 2 = AGTGGTCACA...
umi ACTCTCGTAA = 1093 reads: +385 validated
umi ATTATGTCAA = 3660 reads: -310X +1 -11XX +1 -3XX +1 -7XX +1 -3XX +1 -4XX +42 invalidated
umi CATGCACGGT = 3521 reads: -310X +1 -11XX +1 -3XX +1 -7XX +1 -3XX +1 -4XX +42 invalidated
umi GAATTGCGCA = 1242 reads: -354X +5 -2XX +1 -1XX +2 -1XX +2 -2XX +5 -1XX +1 -2XX +1 -2XX +3 invalidated
umi GATATATTGC = 1565 reads: +385 validated
umi GCCAATCCTT = 731 reads: +385 validated
umi GTTACATCAC = 588 reads: -354X +5 -2XX +1 -1XX +2 -1XX +2 -2XX +5 -1XX +1 -2XX +1 -2XX +3 invalidated
umi TAATTAGGTA = 1438 reads: +385 validated
umi TACTGTCTAG = 1243 reads: +385 validated
umi TATCTTCGTA = 722 reads: +385 validated
umi TGATCAAGAG = 3720 reads: -310X +1 -11XX +1 -3XX +1 -7XX +1 -3XX +1 -4XX +42 invalidated
umi TTCCTCAGTC = 3208 reads: -310X +1 -11XX +1 -3XX +1 -7XX +1 -3XX +1 -4XX +42 invalidated

GOOD CONTIGS

TIG 1[bases=488]
0-46 ==> 0-46 on |506|TRAV38-2/DV8|5'UTR| [len=46] (mis=4)
46-388 ==> 0-342 on |507|TRAV38-2/DV8|L-REGION+V-REGION| [len=349] (mis=0)
393-446 ==> 13-66 on |418|TRAJ32|J-REGION| [len=66] (mis=0)
446-488 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 13 umis using 756 reads
cdr3 = CAYHVATNKLIF at 379, score = 7 + 8
umis assigned: [0, 47, 215, 292, 424, 443, 604, 613, 799, 813] and 3 others
of which 13 are surviving nonsolos
reads assigned: 13098
start codons at 46, 51, 103, 136, 179, 238, 278, 362, 370, 389
confident = true

TIG 2[bases=486]
0-49 ==> 29-78 on |595|TRBV20-1|5'UTR| [len=78] (mis=0)
49-384 ==> 0-335 on |596|TRBV20-1|L-REGION+V-REGION| [len=335] (mis=4)
386-434 ==> 0-48 on |536|TRBJ1-1|J-REGION| [len=48] (mis=4)
434-486 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 6 umis using 458 reads
cdr3 = CSAKMGGTEAFF at 370, score = 7 + 8
umis assigned: [93, 194, 274, 535, 546, 565, 694, 723, 738, 766] and 2 others
of which 12 are surviving nonsolos
reads assigned: 22401
start codons at 49, 187, 223, 229, 242, 302, 382
confident = true
now this is a cell
paired!

GACTCACAGCTGGGGGATGCCGCGATGTATTTCTGTGCTTATCATGTTGCTACAAACAAGCTCATCTTTGGAACTGGCACTCTGCTTGCTGTCCAGCCAA <==> ACCAGTGCCCATCCTGAAGACAGCAGCTTCTACATCTGCAGTGCTAAGATGGGGGGGACTGAAGCTTTCTTTGGACAAGGCACCAGACTCACAGTTGTAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.775 = TCGTACCAGAGAACAG-1

using 71 reads

====================================================================================

graph has 64 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 2[21, 47]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.780 = TCGTACCAGATGCGAC-1

using 3383 reads

====================================================================================

graph has 1425 edges initially, 48 edges after simplification

total ucounts = 247
nonsolo ucounts = 151[2^41, 3^27, 4^16, 5^18, 6^9, 7^9, 8^9, 9^2, 10, 11^8, 12^2, 14, 17, 22, 23, 26, 31, 32, 37, 2439]
surviving nonsolo ucounts = 1[2439]
ids = [198]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.790 = TCGTACCAGGCATTGG-1

using 27 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[2, 25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.801 = TCGTACCAGTGGTCCC-1

using 22 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.803 = TCGTACCAGTGTCCAT-1

using 57 reads

====================================================================================

graph has 48 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[56]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.806 = TCGTACCAGTGTTGAA-1

using 19 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.811 = TCGTACCCAAGACACG-1

using 298 reads

====================================================================================

graph has 224 edges initially, 12 edges after simplification

total ucounts = 19
nonsolo ucounts = 18[2, 3, 4^2, 6, 7^2, 9, 10, 11, 13, 15, 23^2, 25, 28, 42, 65]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.812 = TCGTACCCAAGCGAGT-1

using 185 reads

====================================================================================

graph has 166 edges initially, 6 edges after simplification

total ucounts = 15
nonsolo ucounts = 14[2^3, 4, 8^2, 10, 13, 15, 16, 19, 21, 29, 35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.815 = TCGTACCCACACATGT-1

using 1033 reads

====================================================================================

graph has 880 edges initially, 16 edges after simplification

total ucounts = 172
nonsolo ucounts = 99[2^32, 3^27, 4^13, 5^3, 6^2, 7^3, 9, 12^3, 13, 15^3, 16^2, 18^2, 28, 32, 34, 39, 45^2, 321]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.826 = TCGTACCCAGATCGGA-1

using 26 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.830 = TCGTACCCAGGCAGTA-1

using 400 reads

====================================================================================

graph has 378 edges initially, 18 edges after simplification

total ucounts = 100
nonsolo ucounts = 53[2^12, 3^11, 4^5, 5^7, 6^3, 7^3, 8, 9, 10^2, 11, 15, 20, 21^3, 27, 29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.831 = TCGTACCCAGGGTACA-1

using 23 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.832 = TCGTACCCAGTCAGCC-1

using 47 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[47]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.837 = TCGTACCCATGGGACA-1

using 1893 reads

====================================================================================

graph has 1268 edges initially, 42 edges after simplification

total ucounts = 271
nonsolo ucounts = 149[2^53, 3^25, 4^16, 5^15, 6^8, 7^3, 8^3, 9^4, 10, 11, 12^2, 13^2, 14, 15, 17, 19^2, 20, 24, 26, 34, 43, 44^2, 49, 70, 130, 683]
surviving nonsolo ucounts = 1[683]
ids = [113]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.840 = TCGTACCCATTTCACT-1

using 19 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.842 = TCGTACCGTAATCACC-1

using 25 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.843 = TCGTACCGTAATTGGA-1

using 18 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 2[3, 12]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.844 = TCGTACCGTACAGACG-1

using 1886 reads

====================================================================================

graph has 544 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 2[2, 1881]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.846 = TCGTACCGTAGCCTAT-1

using 23 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[2, 20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.849 = TCGTACCGTATAATGG-1

using 24 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.850 = TCGTACCGTCACACGC-1

using 39 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[39]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.851 = TCGTACCGTCAGATAA-1

using 274 reads

====================================================================================

graph has 110 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[274]
surviving nonsolo ucounts = 1[274]
ids = [0]

====================================================================================

UMI info for barcode TCGTACCGTCAGATAA-1 contig 1 = GGCTTTGTCG...
umi TGCGTCAAAC = 275 reads: +391 validated

GOOD CONTIGS

TIG 1[bases=566]
0-133 ==> 0-133 on |482|TRAV22|5'UTR| [len=133] (mis=0)
133-464 ==> 0-331 on |483|TRAV22|L-REGION+V-REGION| [len=331] (mis=1)
461-524 ==> 0-63 on |443|TRAJ57|J-REGION| [len=63] (mis=2)
524-566 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 1 umis using 9 reads
cdr3 = CAVETQGGSEKLVF at 451, score = 7 + 9
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 270
start codons at 58, 97, 106, 133, 359
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.855 = TCGTACCGTCGCCATG-1

using 611 reads

====================================================================================

graph has 685 edges initially, 30 edges after simplification

total ucounts = 139
nonsolo ucounts = 83[2^22, 3^17, 4^13, 5^5, 6^5, 7^6, 8^2, 9^3, 10, 11, 18, 21, 22^2, 31, 42, 44, 47]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.856 = TCGTACCGTCGCTTCT-1

using 20 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[7, 11]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.869 = TCGTACCGTGTGACCC-1

using 3909 reads

====================================================================================

graph has 2282 edges initially, 56 edges after simplification

total ucounts = 485
nonsolo ucounts = 247[2^80, 3^46, 4^31, 5^17, 6^12, 7^6, 8^7, 9^3, 10^6, 11^4, 12^4, 13^5, 14^2, 15^2, 16, 17^2, 18, 19^3, 20^2, 21, 22, 23, 25, 29, 33, 39, 40, 42, 55, 63, 91, 2044]
surviving nonsolo ucounts = 2[63, 2044]
ids = [109, 287]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.882 = TCGTACCTCAACGCTA-1

using 27 reads

====================================================================================

graph has 56 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.884 = TCGTACCTCACAACGT-1

using 47 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[47]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.890 = TCGTACCTCAGTCCCT-1

using 34 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.901 = TCGTACCTCCGATATG-1

using 49 reads

====================================================================================

graph has 52 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[2, 45]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.904 = TCGTACCTCCTCGCAT-1

using 34 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.907 = TCGTACCTCGCCAAAT-1

using 204 reads

====================================================================================

graph has 164 edges initially, 8 edges after simplification

total ucounts = 23
nonsolo ucounts = 19[2^2, 3^2, 4^4, 5^2, 8, 10, 11^2, 12, 13, 18, 22, 59]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.908 = TCGTACCTCGCCGTGA-1

using 99 reads

====================================================================================

graph has 136 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 8[2, 4, 10, 12, 15, 17, 19, 20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.915 = TCGTACCTCTAACTTC-1

using 37 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[11, 26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.917 = TCGTACCTCTCTGAGA-1

using 22 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.918 = TCGTACCTCTTACCTA-1

using 114 reads

====================================================================================

graph has 108 edges initially, 6 edges after simplification

total ucounts = 31
nonsolo ucounts = 21[2^9, 3^3, 4, 5^3, 6^2, 7, 10, 29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.925 = TCGTAGAAGAGTAAGG-1

using 15 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.936 = TCGTAGAAGGACAGCT-1

using 20 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.939 = TCGTAGAAGGCAATTA-1

using 10 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[10]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.942 = TCGTAGAAGGTGTGGT-1

using 24 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.944 = TCGTAGAAGTGTCCAT-1

using 697 reads

====================================================================================

graph has 280 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[697]
surviving nonsolo ucounts = 1[697]
ids = [0]

====================================================================================

UMI info for barcode TCGTAGAAGTGTCCAT-1 contig 1 = ATGCTCACAG...
umi GATGGTGTCC = 702 reads: +406 validated

GOOD CONTIGS

TIG 1[bases=510]
0-52 ==> 10-62 on |565|TRBV12-3|5'UTR| [len=62] (mis=0)
52-399 ==> 0-347 on |566|TRBV12-3|L-REGION+V-REGION| [len=347] (mis=5)
407-458 ==> 0-51 on |539|TRBJ1-4|J-REGION| [len=51] (mis=5)
458-510 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 1 umis using 47 reads
cdr3 = CASSPLAGWFSEKLFF at 382, score = 8 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 688
start codons at 0, 52, 110, 137, 151, 174, 223, 226, 275, 286, 313, 320
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.948 = TCGTAGACAACACCCG-1

using 205 reads

====================================================================================

graph has 158 edges initially, 8 edges after simplification

total ucounts = 13
nonsolo ucounts = 12[2^2, 3, 4^2, 5, 8, 14, 22, 29, 55, 56]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.949 = TCGTAGACAAGAGGCT-1

using 2464 reads

====================================================================================

graph has 1694 edges initially, 28 edges after simplification

total ucounts = 231
nonsolo ucounts = 155[2^49, 3^28, 4^25, 5^17, 6^11, 7^3, 8^2, 9^3, 11^2, 12^2, 13, 15, 27, 28, 32, 37, 52, 96, 136, 162, 170, 333, 744]
surviving nonsolo ucounts = 6[96, 136, 162, 170, 333, 744]
ids = [64, 200, 190, 10, 105, 47]

====================================================================================

UMI info for barcode TCGTAGACAAGAGGCT-1 contig 1 = CCGGTGTCGG...
umi AAGCGTTTAT = 167 reads: +295 -1 +41 -3 +1 -2 +4 -1 +2 -1 +1 -1 +2 -1 +1 -1 +1 -1 +2 -2 +4 -1 +5 -1 +9 -1 +15 non-validated
umi CGTCTTACCG = 333 reads: +400 validated
umi TCCGGTAGGG = 155 reads: +321 -3 +56 -5 +15 non-validated
umi TGACATCTGT = 137 reads: +339 -46 +15 non-validated

UMI info for barcode TCGTAGACAAGAGGCT-1 contig 2 = TTTCTTTCTT...
umi AGTTCAGCGG = 744 reads: +400 validated
umi CAAGAATCCT = 56 reads: +224 -1 +87 -84 +4 non-validated

GOOD CONTIGS

TIG 1[bases=618]
0-176 ==> 0-176 on |496|TRAV3|5'UTR| [len=176] (mis=1)
176-519 ==> 0-343 on |497|TRAV3|L-REGION+V-REGION| [len=343] (mis=1)
519-576 ==> 0-57 on |419|TRAJ33|J-REGION| [len=57] (mis=2)
576-618 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 1 umis using 8 reads
cdr3 = CAVRDRGSNYQLIW at 503, score = 8 + 9
umis assigned: [10, 105, 190, 200]
of which 4 are surviving nonsolos
reads assigned: 775
start codons at 176, 197, 206, 298, 339, 414
confident = true

TIG 2[bases=472]
0-20 ==> 0-20 on |612|TRBV28|5'UTR| [len=20] (mis=1)
20-364 ==> 0-344 on |613|TRBV28|L-REGION+V-REGION| [len=344] (mis=5)
372-420 ==> 0-48 on |548|TRBJ2-5|J-REGION| [len=48] (mis=1)
420-472 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 1 umis using 41 reads
cdr3 = CASRLTTGNQETQYF at 347, score = 6 + 7
umis assigned: [47, 64]
of which 2 are surviving nonsolos
reads assigned: 789
start codons at 20, 78, 142, 155, 162, 170, 225, 228, 236, 338
confident = true
now this is a cell
paired!

CTTGTGAGCGACTCCGCTTTGTACTTCTGTGCTGTGAGAGACCGGGGTAGCAACTATCAGTTAATCTGGGGCGCTGGGACCAAGCTAATTATAAAGCCAG <==> AGCACCAACCAGACATCTATGTACCTCTGTGCCAGCAGACTCACTACTGGGAATCAAGAGACCCAGTACTTCGGGCCAGGCACGCGGCTCCTGGTGCTCG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.964 = TCGTAGACAGCTGTAT-1

using 36 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.970 = TCGTAGACAGTCGTGC-1

using 34 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.974 = TCGTAGACATCTCGCT-1

using 76 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[76]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.980 = TCGTAGACATTTCACT-1

using 20 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.988 = TCGTAGAGTATCTGCA-1

using 1408 reads

====================================================================================

graph has 1864 edges initially, 20 edges after simplification

total ucounts = 309
nonsolo ucounts = 213[2^52, 3^42, 4^27, 5^19, 6^13, 7^9, 8^5, 9^10, 10^6, 11^8, 12^3, 13^3, 15^3, 16^2, 17^2, 18, 20, 22, 24, 26^2, 39, 44, 55]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.989 = TCGTAGAGTCAAACTC-1

using 45 reads

====================================================================================

graph has 46 edges initially, 4 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[16, 29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.994 = TCGTAGAGTCATATCG-1

using 4821 reads

====================================================================================

graph has 3450 edges initially, 48 edges after simplification

total ucounts = 490
nonsolo ucounts = 356[2^83, 3^49, 4^48, 5^44, 6^30, 7^22, 8^19, 9^11, 10^5, 11^8, 12^10, 13^4, 14^3, 15, 16^3, 18^2, 20, 24, 28, 33, 36, 42, 45, 64, 84, 145, 310, 473, 666, 955]
surviving nonsolo ucounts = 7[14, 33, 145, 310, 473, 666, 955]
ids = [185, 437, 14, 360, 305, 321, 329]

====================================================================================

UMI info for barcode TCGTAGAGTCATATCG-1 contig 1 = ATTATTTTTT...
umi AAGTTGTTGA = 130 reads: -346 +5 -1 +48 non-validated
umi CGGTAACCCT = 14 reads: +67 -55 +88 -44 +5 -1 +87 -38 +15 non-validated
umi GTCCTGCTTT = 957 reads: +400 validated
umi TGTAGTGTTG = 33 reads: +205 -4 +57 -1 +1 -66 +18 -1 +47 non-validated

UMI info for barcode TCGTAGAGTCATATCG-1 contig 2 = ATTCTTTCTT...
umi GGCCGGCCTT = 417 reads: +360 -1 +6 -1 +6 -10 +10 non-validated
umi TACCTACCCC = 313 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=498]
0-56 ==> 0-56 on |461|TRAV12-3|5'UTR| [len=56] (mis=0)
56-399 ==> 0-343 on |462|TRAV12-3|L-REGION+V-REGION| [len=343] (mis=0)
401-456 ==> 0-55 on |406|TRAJ21|J-REGION| [len=55] (mis=1)
456-498 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 1 umis using 63 reads
cdr3 = CAMSADNFNKFYF at 386, score = 9 + 7
umis assigned: [14, 185, 329, 437]
of which 4 are surviving nonsolos
reads assigned: 1113
start codons at 56, 59, 221, 263, 294, 392, 444
confident = true

TIG 2[bases=466]
0-20 ==> 0-20 on |612|TRBV28|5'UTR| [len=20] (mis=0)
20-358 ==> 0-338 on |613|TRBV28|L-REGION+V-REGION| [len=344] (mis=0)
367-414 ==> 0-47 on |550|TRBJ2-7|J-REGION| [len=47] (mis=2)
414-466 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 1 umis using 27 reads
cdr3 = CASRSTGPYEQYF at 347, score = 6 + 8
umis assigned: [305, 360]
of which 2 are surviving nonsolos
reads assigned: 711
start codons at 20, 78, 142, 155, 162, 170, 225, 228, 236, 338
confident = true
now this is a cell
paired!

TCACAGCCCAGTGATTCAGCCACCTACCTCTGTGCAATGAGCGCGGACAACTTCAACAAATTTTACTTTGGATCTGGGACCAAACTCAATGTAAAACCAA <==> TCCGCCAGCACCAACCAGACATCTATGTACCTCTGTGCCAGCAGGAGCACAGGGCCCTACGAGCAGTACTTCGGGCCGGGCACCAGGCTCACGGTCACAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.995 = TCGTAGAGTCATCGGC-1

using 1086 reads

====================================================================================

graph has 334 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 1[1083]
surviving nonsolo ucounts = 1[1083]
ids = [3]

====================================================================================

UMI info for barcode TCGTAGAGTCATCGGC-1 contig 1 = GGGATCCTTC...
umi CTTCAGTCTT = 1085 reads: +397 validated

GOOD CONTIGS

TIG 1[bases=471]
22-364 ==> 0-342 on |733|TRBV11-2|L-REGION+V-REGION| [len=342] (mis=1)
372-419 ==> 0-47 on |550|TRBJ2-7|J-REGION| [len=47] (mis=1)
419-471 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 1 umis using 86 reads
cdr3 = CASSLLGSYEQYF at 352, score = 8 + 8
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 1069
start codons at 22, 164, 245
confident = false
NOT paired!

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BARCODE 84.998 = TCGTAGAGTCGGCTCA-1

using 233 reads

====================================================================================

graph has 308 edges initially, 4 edges after simplification

total ucounts = 123
nonsolo ucounts = 45[2^25, 3^9, 4^5, 5^2, 9^2, 13, 17]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

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BARCODE 84.1002 = TCGTAGAGTGAAAGAG-1

using 46 reads

====================================================================================

graph has 56 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 8[2^2, 3^2, 4, 5, 6, 18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!
sorting bam, mem = 0.12
running samtools sort -l 8G -o /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_T_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk84-ucc6a7a72e6/files/contig_bam_sorted.bam /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_T_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk84-ucc6a7a72e6/files/contig_bam.bam

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53.823 seconds used processing barcodes, peak mem = 0.27
