[stdout]

starting vdj_asm_asm, mem = 0.01
available mem = 827.96 GB
/scratch/etanis/software/cellranger-6.1.2/lib/bin/cr_vdj martian assembly main /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_T_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk78-ucc6a7a72e6 /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_T_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk78-ucc6a7a72e6/files /scratch/etanis/ST-118/multi/dimitri-multi/journal/SC_MULTI_CS.SC_MULTI_CORE.MULTI_GEM_WELL_PROCESSOR.VDJ_T_GEM_WELL_PROCESSOR.SC_VDJ_CONTIG_ASSEMBLER.ASSEMBLE_VDJ.fork0.chnk78.ucc6a7a72e6
n50_n50_rpu = 2159

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.0 = TACTTGTCAGATCTGT-1

using 31 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.3 = TACTTGTCAGCCACCA-1

using 47 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[47]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.4 = TACTTGTCAGCTTAAC-1

using 127 reads

====================================================================================

graph has 116 edges initially, 2 edges after simplification

total ucounts = 17
nonsolo ucounts = 11[2^2, 4, 5^2, 6, 13, 14^2, 21, 35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.5 = TACTTGTCAGGAATGC-1

using 70 reads

====================================================================================

graph has 66 edges initially, 4 edges after simplification

total ucounts = 11
nonsolo ucounts = 6[2^2, 4, 5, 24, 28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.11 = TACTTGTCATCTCCCA-1

using 16 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[16]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.14 = TACTTGTCATGGTTGT-1

using 117 reads

====================================================================================

graph has 52 edges initially, 4 edges after simplification

total ucounts = 7
nonsolo ucounts = 6[2, 7, 12, 14, 18, 63]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.16 = TACTTGTCATGTCTCC-1

using 41545 reads

====================================================================================

graph has 15353 edges initially, 312 edges after simplification

total ucounts = 1163
nonsolo ucounts = 884[2^113, 3^77, 4^72, 5^72, 6^48, 7^43, 8^35, 9^50, 10^40, 11^23, 12^23, 13^17, 14^19, 15^11, 16^16, 17^19, 18^11, 19^12, 20^13, 21^18, 22^14, 23^8, 24^8, 25^9, 26^5, 27^9, 28, 29^8, 30^6, 31^6, 32^2, 33^3, 34^5, 35^2, 37^5, 38^3, 39^4, 40^2, 41^2, 42, 43^3, 44, 46, 47, 48^2, 50, 51^2, 52, 54, 56, 60^2, 65^2, 67, 68, 69^2, 70, 71, 72^2, 77, 78^2, 80, 88, 93, 97^2, 104, 348, 736, 952, 1259, 1304, 1384, 1412, 1572, 1609, 2107, 2924, 3038, 3146, 8581]
surviving nonsolo ucounts = 14[348, 736, 952, 1259, 1304, 1384, 1412, 1572, 1609, 2107, 2924, 3038, 3146, 8581]
ids = [500, 232, 208, 750, 138, 387, 1105, 652, 78, 511, ...]

====================================================================================

UMI info for barcode TACTTGTCATGTCTCC-1 contig 1 = AGACCAGAAT...
umi ATAGCGTTAT = 950 reads: +403 validated
umi ATCTCTACTC = 729 reads: +403 validated
umi CGTTACGTAC = 3082 reads: +403 validated
umi GGGGCACGTT = 7248 reads: -366 +5 -1XX +2 -1XX +2 -1XX +2 -1XX +6 -1XX +4 -1XX +2 -1XX +2 -4XX +1 invalidated
umi TTCTTGATCT = 1405 reads: +403 validated

UMI info for barcode TACTTGTCATGTCTCC-1 contig 2 = CTTCTGCAGA...
umi ACAGCAAGCA = 1596 reads: +376 validated
umi ACTTAGGGTT = 1337 reads: +232 -2XX +128 -2XX +6 -3XX +3 invalidated
umi CCGATCGGCA = 1377 reads: +376 validated
umi CTCACTTCTT = 334 reads: +373 -1 +2 non-validated
umi CTCCTAACCC = 2093 reads: +376 validated
umi GCGTTACCGC = 1581 reads: +376 validated
umi GTCCCAGCAA = 1256 reads: +376 validated
umi TTTGTTCTCT = 2929 reads: +376 validated

GOOD CONTIGS

TIG 1[bases=502]
0-47 ==> 0-47 on |616|TRBV3-1|5'UTR| [len=47] (mis=0)
47-391 ==> 0-344 on |617|TRBV3-1|L-REGION+V-REGION| [len=344] (mis=2)
403-450 ==> 0-47 on |550|TRBJ2-7|J-REGION| [len=47] (mis=5)
450-502 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 4 umis using 387 reads
cdr3 = CASSQTVAGGPGEQYF at 374, score = 9 + 8
umis assigned: [208, 232, 473, 705, 1105]
of which 5 are surviving nonsolos
reads assigned: 13201
start codons at 47, 146, 169, 189, 197, 239, 273
confident = true

TIG 2[bases=484]
0-66 ==> 0-66 on |451|TRAV1-1|5'UTR| [len=66] (mis=0)
66-392 ==> 0-326 on |452|TRAV1-1|L-REGION+V-REGION| [len=326] (mis=4)
382-442 ==> 0-60 on |414|TRAJ29|J-REGION| [len=60] (mis=4)
442-484 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 8 umis using 817 reads
cdr3 = CAVSGNTPLVF at 378, score = 7 + 9
umis assigned: [78, 138, 387, 500, 511, 652, 750, 1147]
of which 8 are surviving nonsolos
reads assigned: 12224
start codons at 33, 49, 66, 88, 96, 102, 205, 229, 232, 262, 271, 325, 354
confident = true

REJECT CONTIGS

TIG 1[bases=417]
5-22 ==> 171-188 on segment before TRAV6 exon 2 [len=188] (mis=0)
22-310 ==> 109-397 on |516|TRAV6|L-REGION+V-REGION| [len=397] (mis=5)
313-375 ==> 0-62 on |423|TRAJ37|J-REGION| [len=62] (mis=3)
375-417 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
cdr3 = CSPYGASSNTGKLIF at 299, score = 3 + 8
umis assigned: [115, 741, 892]
of which 1 are surviving nonsolos
reads assigned: 3097
start codons at 187, 309
confident = false
VJ delta = 22
not full
not full
now this is a cell
paired!

CAGGAGCTCCAGATGAAAGACTCTGCCTCTTACTTCTGCGCTGTTTCAGGAAACACACCTCTTGTCTTTGGAAAGGGCACAAGACTTTCTGTGATTGCAA <==> CTTGGTGACTCTGCTGTGTATTTCTGTGCCAGCAGCCAAACGGTAGCGGGAGGGCCGGGCGAGCAGTACTTCGGGCCGGGCACCAGGCTCACGGTCACAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.19 = TACTTGTCATTCCTGC-1

using 235 reads

====================================================================================

graph has 166 edges initially, 8 edges after simplification

total ucounts = 9
nonsolo ucounts = 7[2, 6, 11, 34, 36, 72^2]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.20 = TACTTGTCATTCTCAT-1

using 49 reads

====================================================================================

graph has 52 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 6[2, 3, 6, 7, 8, 20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.22 = TACTTGTGTACAGTTC-1

using 31 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.28 = TACTTGTGTATCTGCA-1

using 14 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[14]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.30 = TACTTGTGTATTCGTG-1

using 31 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.32 = TACTTGTGTCAAAGCG-1

using 70 reads

====================================================================================

graph has 46 edges initially, 4 edges after simplification

total ucounts = 8
nonsolo ucounts = 6[2, 5^3, 22, 29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.34 = TACTTGTGTCAGCTAT-1

using 14 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[14]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.36 = TACTTGTGTCATTAGC-1

using 98 reads

====================================================================================

graph has 74 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 4[2, 8, 12, 73]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.37 = TACTTGTGTCCAGTGC-1

using 67 reads

====================================================================================

graph has 78 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 8[3^4, 4^2, 5, 40]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.39 = TACTTGTGTCCATGAT-1

using 34 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.40 = TACTTGTGTCCTGCTT-1

using 88 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[87]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.42 = TACTTGTGTCGAACAG-1

using 13003 reads

====================================================================================

graph has 9978 edges initially, 368 edges after simplification

total ucounts = 1169
nonsolo ucounts = 887[2^127, 3^77, 4^80, 5^59, 6^38, 7^42, 8^39, 9^29, 10^33, 11^33, 12^21, 13^25, 14^25, 15^23, 16^14, 17^18, 18^10, 19^23, 20^10, 21^6, 22^12, 23^10, 24^12, 25^11, 26^7, 27^7, 28^9, 29^5, 30^3, 31^7, 32^5, 33, 34^4, 35, 36^3, 38^3, 39^2, 40, 41^3, 42, 44, 46^4, 47^3, 48^2, 52, 53, 54^3, 55^2, 57^2, 59, 60^2, 61, 62, 63, 64, 67, 68^2, 70, 71, 73^3, 74, 77, 78, 80, 81, 84, 87, 90, 91, 93, 98, 101, 112, 993]
surviving nonsolo ucounts = 3[2, 10, 993]
ids = [322, 567, 400]

====================================================================================

REJECT CONTIGS

TIG 1[bases=441]
5-53 ==> 0-48 on |548|TRBJ2-5|J-REGION| [len=48] (mis=0)
53-125 ==> 0-72 on segment before TRBJ2-6 exon 1 [len=72] (mis=0)
178-342 ==> 0-164 on segment before TRBJ2-7 exon 1 [len=164] (mis=2)
389-441 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
umis assigned: [400]
of which 1 are surviving nonsolos
reads assigned: 979
start codons at 321
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.49 = TACTTGTGTCTCAACA-1

using 48 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[48]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.52 = TACTTGTGTGAGGGAG-1

using 133 reads

====================================================================================

graph has 120 edges initially, 4 edges after simplification

total ucounts = 10
nonsolo ucounts = 7[2, 6^2, 10, 24, 39, 43]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.54 = TACTTGTGTGATGTCT-1

using 18 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.55 = TACTTGTGTGCAACGA-1

using 34 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.56 = TACTTGTGTGGTAACG-1

using 34 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 5[2^2, 3, 4, 20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.57 = TACTTGTGTGTCCTCT-1

using 20 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.61 = TACTTGTGTTACTGAC-1

using 321 reads

====================================================================================

graph has 216 edges initially, 8 edges after simplification

total ucounts = 13
nonsolo ucounts = 12[3^2, 7, 8, 11, 12, 15, 20, 44, 50, 72, 75]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.62 = TACTTGTGTTCGGCAC-1

using 121 reads

====================================================================================

graph has 76 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 3[2, 8, 108]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.63 = TACTTGTGTTCGGGCT-1

using 89 reads

====================================================================================

graph has 72 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 6[2, 5^3, 6, 64]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.67 = TACTTGTTCAAACCAC-1

using 124 reads

====================================================================================

graph has 110 edges initially, 4 edges after simplification

total ucounts = 16
nonsolo ucounts = 9[2^2, 3, 6, 11, 16, 24^2, 29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.72 = TACTTGTTCACCCGAG-1

using 72 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[72]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.73 = TACTTGTTCACCCTCA-1

using 48 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[2, 46]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.74 = TACTTGTTCACTATTC-1

using 77 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[2, 3, 71]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.76 = TACTTGTTCACTTCAT-1

using 108 reads

====================================================================================

graph has 64 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 8[2, 3, 4, 5, 8^2, 9, 67]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.77 = TACTTGTTCAGCACAT-1

using 18 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.78 = TACTTGTTCAGCATGT-1

using 107 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[106]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.81 = TACTTGTTCAGGTAAA-1

using 29 reads

====================================================================================

graph has 62 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.84 = TACTTGTTCATCTGCC-1

using 92 reads

====================================================================================

graph has 86 edges initially, 4 edges after simplification

total ucounts = 9
nonsolo ucounts = 6[4, 7, 11, 13, 17, 37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.85 = TACTTGTTCATGTCTT-1

using 33 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.86 = TACTTGTTCATTATCC-1

using 73 reads

====================================================================================

graph has 64 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 7[2^2, 3^2, 9, 12, 39]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.88 = TACTTGTTCCAATGGT-1

using 139 reads

====================================================================================

graph has 104 edges initially, 4 edges after simplification

total ucounts = 12
nonsolo ucounts = 8[2, 5, 8^2, 9, 13, 21, 69]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.90 = TACTTGTTCCCAGGTG-1

using 90 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[90]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.93 = TACTTGTTCCGAGCCA-1

using 51 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 1[49]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.94 = TACTTGTTCCGCGGTA-1

using 3244 reads

====================================================================================

graph has 2810 edges initially, 104 edges after simplification

total ucounts = 421
nonsolo ucounts = 320[2^57, 3^40, 4^41, 5^16, 6^26, 7^13, 8^14, 9^10, 10^16, 11^9, 12^4, 13^10, 14^7, 15^6, 16^3, 17^5, 18^3, 19^5, 20^3, 21, 22, 24^4, 25, 26, 27^3, 29, 30^2, 31^3, 32, 34, 38, 39^3, 41, 43, 44, 50, 54, 58, 84, 96, 104]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.96 = TACTTGTTCCGTTGCT-1

using 379 reads

====================================================================================

graph has 164 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[379]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.97 = TACTTGTTCCTACAGA-1

using 39 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[39]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.98 = TACTTGTTCCTTGACC-1

using 69 reads

====================================================================================

graph has 26 edges initially, 4 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[34, 35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.99 = TACTTGTTCGATCCCT-1

using 25 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.104 = TACTTGTTCGCGGATC-1

using 97 reads

====================================================================================

graph has 68 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 10[2, 4, 5, 8^2, 10^2, 11, 18, 21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.109 = TACTTGTTCTCCTATA-1

using 134 reads

====================================================================================

graph has 52 edges initially, 4 edges after simplification

total ucounts = 6
nonsolo ucounts = 5[6, 8, 9, 41, 69]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.112 = TACTTGTTCTGAAAGA-1

using 99 reads

====================================================================================

graph has 66 edges initially, 4 edges after simplification

total ucounts = 9
nonsolo ucounts = 7[2, 3, 4^2, 6, 22, 56]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.113 = TACTTGTTCTGACCTC-1

using 87 reads

====================================================================================

graph has 64 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[87]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.114 = TACTTGTTCTGCTGCT-1

using 59 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[59]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.115 = TACTTGTTCTGGCGAC-1

using 80 reads

====================================================================================

graph has 48 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[80]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.117 = TACTTGTTCTGTACGA-1

using 206 reads

====================================================================================

graph has 190 edges initially, 4 edges after simplification

total ucounts = 18
nonsolo ucounts = 13[2, 3^3, 4, 9, 10, 21, 22, 24, 26, 35, 39]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.119 = TACTTGTTCTTAGCCC-1

using 59 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[58]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.120 = TACTTGTTCTTATCTG-1

using 37 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[5, 32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.121 = TACTTGTTCTTCATGT-1

using 18 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.131 = TAGACCAAGATAGGAG-1

using 39 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[39]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.138 = TAGACCAAGCGCCTTG-1

using 52 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[52]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.139 = TAGACCAAGCGTCTAT-1

using 64 reads

====================================================================================

graph has 70 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[64]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.141 = TAGACCAAGCGTTGCC-1

using 16 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.143 = TAGACCAAGCTACCGC-1

using 77 reads

====================================================================================

graph has 74 edges initially, 2 edges after simplification

total ucounts = 13
nonsolo ucounts = 9[2^2, 3^2, 5, 8^2, 20, 22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.145 = TAGACCAAGCTTATCG-1

using 75 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[75]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.146 = TAGACCAAGCTTCGCG-1

using 51 reads

====================================================================================

graph has 56 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 5[4^2, 6, 14, 20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.151 = TAGACCAAGGGATACC-1

using 59 reads

====================================================================================

graph has 56 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 8[3, 4, 5, 6^2, 7, 8, 19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.157 = TAGACCAAGTATCGAA-1

using 27 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[2, 3, 20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.158 = TAGACCAAGTCATCCA-1

using 48 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[48]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.161 = TAGACCAAGTGCGTGA-1

using 13270 reads

====================================================================================

graph has 5666 edges initially, 78 edges after simplification

total ucounts = 255
nonsolo ucounts = 187[2^25, 3^14, 4^14, 5^12, 6^8, 7^9, 8^7, 9^6, 10^5, 11, 12^9, 13^9, 14^5, 15^2, 16^9, 17^3, 18^5, 19^6, 20^3, 21^3, 22, 25^3, 27, 28, 29, 30, 32, 33, 34, 36, 37, 39, 40, 42, 50, 52, 53, 59, 62^2, 63, 67, 107, 113, 571, 1120, 1785, 2017, 2066, 3114]
surviving nonsolo ucounts = 4[571, 1785, 2017, 2066]
ids = [192, 92, 196, 236]

====================================================================================

UMI info for barcode TAGACCAAGTGCGTGA-1 contig 1 = ATGCTCACAG...
umi ATAGACCTCC = 72 reads: -379X +1 -5X +3 -13X +2 invalidated
umi CCTCTTTCCT = 1793 reads: +403 validated
umi TATTGTCGGG = 499 reads: -372X +5 -4X +2 -1X +1 -3X +5 -1XX +1 -2XX +1 -2XX +3 invalidated
umi TCATCTATTT = 2036 reads: +403 validated

GOOD CONTIGS

TIG 1[bases=507]
0-52 ==> 10-62 on |565|TRBV12-3|5'UTR| [len=62] (mis=0)
52-399 ==> 0-347 on |566|TRBV12-3|L-REGION+V-REGION| [len=347] (mis=5)
407-455 ==> 0-48 on |536|TRBJ1-1|J-REGION| [len=48] (mis=5)
455-507 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 2 umis using 244 reads
cdr3 = CASSDQMSGQIEAFF at 382, score = 8 + 8
umis assigned: [42, 92, 192, 196]
of which 3 are surviving nonsolos
reads assigned: 4310
start codons at 0, 52, 110, 137, 151, 174, 223, 226, 275, 286, 313, 320, 400
confident = true
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.162 = TAGACCAAGTGCTGCC-1

using 1034 reads

====================================================================================

graph has 1054 edges initially, 28 edges after simplification

total ucounts = 158
nonsolo ucounts = 112[2^16, 3^20, 4^11, 5^12, 6^10, 7^3, 8^7, 9^6, 10^3, 11^2, 12^4, 13, 14, 16^3, 18^2, 19, 22, 26^2, 27, 31, 33, 39, 40, 55, 72]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.166 = TAGACCAAGTTGCAGG-1

using 58 reads

====================================================================================

graph has 66 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 3[9, 15, 34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.168 = TAGACCAAGTTTCCTT-1

using 99 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[99]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.169 = TAGACCACAAACTGCT-1

using 42 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[42]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.170 = TAGACCACAACACGCC-1

using 26 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.172 = TAGACCACAACTTGAC-1

using 35 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.173 = TAGACCACAAGCCATT-1

using 49 reads

====================================================================================

graph has 50 edges initially, 4 edges after simplification

total ucounts = 8
nonsolo ucounts = 5[5, 7, 9, 10, 15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.176 = TAGACCACAATCCAAC-1

using 57 reads

====================================================================================

graph has 80 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 7[3^2, 5, 7^2, 10, 21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.177 = TAGACCACAATGAAAC-1

using 66 reads

====================================================================================

graph has 80 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 5[3, 4, 9, 22, 27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.178 = TAGACCACACAACTGT-1

using 16 reads

====================================================================================

graph has 4 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[16]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.180 = TAGACCACACACCGCA-1

using 49 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[2, 45]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.183 = TAGACCACACATGACT-1

using 55 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[55]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.186 = TAGACCACACCGAAAG-1

using 21 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.191 = TAGACCACACTACAGT-1

using 31 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 5[2^3, 11, 14]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.198 = TAGACCACAGCTCGAC-1

using 26 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.199 = TAGACCACAGCTCGCA-1

using 27 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.202 = TAGACCACAGGTCCAC-1

using 71060 reads

====================================================================================

graph has 18559 edges initially, 262 edges after simplification

total ucounts = 956
nonsolo ucounts = 695[2^97, 3^74, 4^53, 5^53, 6^44, 7^24, 8^26, 9^36, 10^28, 11^26, 12^19, 13^6, 14^25, 15^17, 16^7, 17^9, 18^13, 19^10, 20^10, 21^2, 22^6, 23^7, 24^7, 25^6, 26^6, 27, 28^3, 29^4, 30^4, 31^2, 32^3, 33, 34^3, 35, 36, 38^2, 44, 45^2, 46, 47, 48, 49^2, 50, 52, 53^3, 54, 58, 59, 63, 64^2, 68^3, 71, 75, 77^2, 79, 81, 86^2, 91^2, 92, 93, 94, 111, 202, 227, 273, 340, 471, 698, 730, 911, 951, 1007, 1138, 1685, 1787, 1912, 2540, 2791, 3150, 3823, 4048, 4624, 6583, 10283, 12402]
surviving nonsolo ucounts = 21[25, 340, 471, 698, 730, 911, 951, 1007, 1138, 1685, 1787, 1912, 2540, 2791, 3150, 3823, 4048, 4624, 6583, 10283, 12402]
ids = [71, 795, 222, 724, 309, 463, 694, 943, 138, 164, ...]

====================================================================================

UMI info for barcode TAGACCACAGGTCCAC-1 contig 1 = GCCAGGTTCA...
umi AGCCTATCGG = 1135 reads: +409 validated
umi AGCTTGGATT = 3824 reads: -262 +1 -3X +143 invalidated
umi CCCGTGACGG = 669 reads: +409 validated
umi TAGGCTGTCA = 955 reads: +409 validated
umi TATTATGGCC = 619 reads: +409 validated
umi TGTCTCGGGC = 1964 reads: +409 validated
umi TTTCGTTAAG = 1009 reads: +409 validated

UMI info for barcode TAGACCACAGGTCCAC-1 contig 2 = GGAGGCAGTG...
umi AGGAACGCAT = 2552 reads: +391 validated
umi AGGTTTGGCA = 1690 reads: +391 validated
umi TATCATAGGG = 1793 reads: +391 validated

GOOD CONTIGS

TIG 1[bases=526]
0-75 ==> 0-75 on |467|TRAV14/DV4|5'UTR| [len=75] (mis=3)
75-425 ==> 0-350 on |468|TRAV14/DV4|L-REGION+V-REGION| [len=350] (mis=7)
421-484 ==> 0-63 on |426|TRAJ4|J-REGION| [len=63] (mis=2)
484-526 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 6 umis using 454 reads
cdr3 = CAMRQFSGGYNKLIF at 408, score = 7 + 9
umis assigned: [138, 146, 309, 694, 724, 848, 943]
of which 7 are surviving nonsolos
reads assigned: 9946
start codons at 75, 165, 208, 229, 267, 295, 307, 399, 414
confident = true

TIG 2[bases=498]
0-55 ==> 23-78 on |595|TRBV20-1|5'UTR| [len=78] (mis=0)
55-382 ==> 0-327 on |596|TRBV20-1|L-REGION+V-REGION| [len=335] (mis=0)
399-446 ==> 6-53 on |541|TRBJ1-6|J-REGION| [len=53] (mis=0)
446-498 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 3 umis using 428 reads
cdr3 = CSIHTGEGNSPLHF at 376, score = 7 + 9
umis assigned: [148, 164, 709]
of which 3 are surviving nonsolos
reads assigned: 5923
start codons at 55, 193, 229, 235, 248, 308
confident = true

REJECT CONTIGS

TIG 1[bases=418]
4-23 ==> 203-222 on segment before TRBJ1-5 exon 1 [len=222] (mis=0)
23-73 ==> 0-50 on |540|TRBJ1-5|J-REGION| [len=50] (mis=0)
366-418 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
umis assigned: [31, 184, 188, 222, 247, 418, 463, 589, 620, 790] and 2 others
of which 10 are surviving nonsolos
reads assigned: 39225
start codons at 10, 13, 49, 74, 118, 179, 261, 277, 336
confident = false
did not find CDR3
now this is a cell
paired!

CTGGGGGACTCAGCAATGTATTTCTGTGCAATGAGACAATTTTCTGGTGGCTACAATAAGCTGATTTTTGGAGCAGGGACCAGGCTGGCTGTACACCCAT <==> GCCCATCCTGAAGACAGCAGCTTCTACATCTGCAGTATTCATACAGGGGAGGGTAATTCACCCCTCCACTTTGGGAACGGGACCAGGCTCACTGTGACAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.203 = TAGACCACAGGTCTCG-1

using 55 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[55]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.206 = TAGACCACAGTACACT-1

using 24 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.208 = TAGACCACATATACGC-1

using 115 reads

====================================================================================

graph has 84 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 5[2, 3^2, 5, 97]
surviving nonsolo ucounts = 1[97]
ids = [5]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.210 = TAGACCACATCAGTCA-1

using 40 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[40]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.215 = TAGACCACATTCCTGC-1

using 39 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[39]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.216 = TAGACCACATTCTTAC-1

using 64 reads

====================================================================================

graph has 60 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[14, 16, 33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.219 = TAGACCAGTAAGTGTA-1

using 1392 reads

====================================================================================

graph has 586 edges initially, 6 edges after simplification

total ucounts = 11
nonsolo ucounts = 8[2^2, 10, 11, 12, 25^2, 1302]
surviving nonsolo ucounts = 1[1302]
ids = [1]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.221 = TAGACCAGTACCATCA-1

using 110 reads

====================================================================================

graph has 72 edges initially, 6 edges after simplification

total ucounts = 9
nonsolo ucounts = 5[3, 11, 12, 33, 47]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.224 = TAGACCAGTATAGGTA-1

using 143 reads

====================================================================================

graph has 88 edges initially, 4 edges after simplification

total ucounts = 13
nonsolo ucounts = 10[2^3, 3^3, 5, 9, 39, 72]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.225 = TAGACCAGTATCTGCA-1

using 50 reads

====================================================================================

graph has 66 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 5[2, 3, 4, 11, 25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.226 = TAGACCAGTATGAATG-1

using 122 reads

====================================================================================

graph has 150 edges initially, 2 edges after simplification

total ucounts = 19
nonsolo ucounts = 13[2^2, 4^2, 5^2, 6, 7^2, 9, 10, 16, 39]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.232 = TAGACCAGTCCTCCAT-1

using 57 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[57]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.234 = TAGACCAGTCTAGAGG-1

using 51 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[13, 38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.236 = TAGACCAGTCTCCATC-1

using 32 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 1[30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.237 = TAGACCAGTCTGATCA-1

using 46 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[46]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.238 = TAGACCAGTGAGGCTA-1

using 12 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[2, 9]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.240 = TAGACCAGTGATGTGG-1

using 75 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[75]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.242 = TAGACCAGTGCTGTAT-1

using 73 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[73]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.249 = TAGACCAGTTCTGTTT-1

using 52 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 3[10, 19, 20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.252 = TAGACCATCAACCATG-1

using 81 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[81]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.254 = TAGACCATCACATGCA-1

using 45 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[45]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.255 = TAGACCATCACGCGGT-1

using 41 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[41]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.259 = TAGACCATCAGCTGGC-1

using 54 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[54]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.262 = TAGACCATCCACTGGG-1

using 89 reads

====================================================================================

graph has 116 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 9[2, 3^2, 5, 6, 8, 13, 23, 25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.264 = TAGACCATCCAGATCA-1

using 50 reads

====================================================================================

graph has 60 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 3[4, 9, 31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.266 = TAGACCATCCATGAGT-1

using 87 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[87]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.268 = TAGACCATCCCTAACC-1

using 84 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[84]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.269 = TAGACCATCCGCAGTG-1

using 9164 reads

====================================================================================

graph has 5025 edges initially, 102 edges after simplification

total ucounts = 608
nonsolo ucounts = 438[2^86, 3^52, 4^40, 5^31, 6^33, 7^22, 8^20, 9^16, 10^14, 11^9, 12^15, 13^10, 14^8, 15^6, 16^7, 17^5, 18^2, 19^8, 20^9, 21^2, 23^5, 24^2, 25^3, 26, 27^3, 28^2, 29, 30, 31^3, 32^2, 33, 35, 36, 38, 41^2, 42, 46, 49^2, 54, 57^2, 66, 71, 88, 95, 105, 109, 4608]
surviving nonsolo ucounts = 1[4608]
ids = [435]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.270 = TAGACCATCCGTCATC-1

using 23042 reads

====================================================================================

graph has 11068 edges initially, 206 edges after simplification

total ucounts = 923
nonsolo ucounts = 699[2^110, 3^83, 4^60, 5^41, 6^37, 7^39, 8^31, 9^27, 10^32, 11^26, 12^23, 13^19, 14^11, 15^20, 16^14, 17^10, 18^9, 19^7, 20^11, 21^2, 22^9, 23^5, 24^4, 25^7, 26^10, 27^7, 28^4, 29^3, 30^2, 31^2, 32, 33, 34, 35, 36^4, 38, 40^3, 42, 43, 44, 48, 54^3, 56^2, 62, 67, 72, 86, 848, 1286, 1573, 1868, 1920, 1970, 2061, 2070, 2260]
surviving nonsolo ucounts = 9[848, 1286, 1573, 1868, 1920, 1970, 2061, 2070, 2260]
ids = [5, 99, 599, 123, 118, 825, 106, 228, 400]

====================================================================================

UMI info for barcode TAGACCATCCGTCATC-1 contig 1 = GATCTTAATT...
umi ACCTAGCGGC = 1283 reads: +391 validated
umi ACTAGGGAAA = 1928 reads: +391 validated
umi ACTCCCTATT = 1826 reads: +391 validated
umi GTCGCATTCA = 1575 reads: +391 validated
umi TGTGTCACTC = 1960 reads: +391 validated

UMI info for barcode TAGACCATCCGTCATC-1 contig 2 = GGGGGTCTTT...
umi ACGACTTGGC = 2071 reads: +400 validated
umi ATTGCTACCA = 2081 reads: +400 validated
umi CTCAGTGTCC = 2274 reads: +400 validated

GOOD CONTIGS

TIG 1[bases=456]
0-23 ==> 0-23 on |463|TRAV13-1|5'UTR| [len=23] (mis=0)
23-360 ==> 0-337 on |464|TRAV13-1|L-REGION+V-REGION| [len=337] (mis=6)
352-414 ==> 0-62 on |446|TRAJ6|J-REGION| [len=62] (mis=3)
414-456 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 5 umis using 541 reads
cdr3 = CASTGGSYIPTF at 347, score = 9 + 7
umis assigned: [99, 118, 123, 599, 825]
of which 5 are surviving nonsolos
reads assigned: 8431
start codons at 23, 78, 87, 237
confident = true

TIG 2[bases=475]
23-367 ==> 0-344 on |613|TRBV28|L-REGION+V-REGION| [len=344] (mis=1)
375-423 ==> 0-48 on |537|TRBJ1-2|J-REGION| [len=48] (mis=2)
423-475 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 3 umis using 492 reads
cdr3 = CASSLSPRADYGYTF at 350, score = 6 + 9
umis assigned: [106, 228, 400]
of which 3 are surviving nonsolos
reads assigned: 6329
start codons at 23, 81, 145, 158, 165, 173, 228, 231, 239, 341, 381
confident = true

REJECT CONTIGS

TIG 1[bases=501]
4-68 ==> 3661-3725 on segment before TRBV7-4 exon 1 [len=3725] (mis=0)
60-81 ==> 1110-1131 on segment before TRBV6-2 exon 1 [len=3166] (mis=1)
68-415 ==> 0-347 on |680|TRBV7-4|L-REGION+V-REGION| [len=347] (mis=1)
422-470 ==> 0-48 on |548|TRBJ2-5|J-REGION| [len=48] (mis=4)
470-501 ==> 0-31 on |534|TRBC2|C-REGION| [len=536] (mis=0)
cdr3 = CASSLVLIR at 398, score = 8 + 4
umis assigned: [5]
of which 1 are surviving nonsolos
reads assigned: 832
start codons at 68, 177, 210, 279
confident = false
not full
frameshifted full length transcript of length 501
VJ delta = 0
not full
now this is a cell
paired!

GAGACCCAACCTGAAGACTCGGCTGTCTACTTCTGTGCCTCAACAGGAGGAAGCTACATACCTACATTTGGAAGAGGAACCAGCCTTATTGTTCATCCGT <==> AGCACCAACCAGACATCTATGTACCTCTGTGCCAGCAGTTTATCCCCCCGGGCCGACTATGGCTACACCTTCGGTTCGGGGACCAGGTTAACCGTTGTAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.271 = TAGACCATCCGTTGTC-1

using 27 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[7, 20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.272 = TAGACCATCCTCCTAG-1

using 42 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[42]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.277 = TAGACCATCGACAGCC-1

using 25 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[3, 22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.278 = TAGACCATCGGAATCT-1

using 66 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[66]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.282 = TAGACCATCGTGGACC-1

using 49 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 2[7, 39]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.283 = TAGACCATCGTTACAG-1

using 77 reads

====================================================================================

graph has 82 edges initially, 2 edges after simplification

total ucounts = 12
nonsolo ucounts = 8[3^3, 4^2, 5, 18, 33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.284 = TAGACCATCTAACTCT-1

using 56 reads

====================================================================================

graph has 74 edges initially, 2 edges after simplification

total ucounts = 16
nonsolo ucounts = 6[2, 5^2, 8^2, 18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.285 = TAGACCATCTAACTGG-1

using 51 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[8, 17, 25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.288 = TAGACCATCTAGAGTC-1

using 69 reads

====================================================================================

graph has 86 edges initially, 2 edges after simplification

total ucounts = 13
nonsolo ucounts = 10[2^3, 3, 4, 5, 9, 11, 14^2]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.291 = TAGACCATCTGTACGA-1

using 44 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[44]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.295 = TAGACCATCTTATCTG-1

using 48 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[48]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.296 = TAGACCATCTTCAACT-1

using 48 reads

====================================================================================

graph has 42 edges initially, 4 edges after simplification

total ucounts = 6
nonsolo ucounts = 2[21, 23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.299 = TAGACCATCTTTCCTC-1

using 80 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[80]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.300 = TAGAGCTAGACAGGCT-1

using 19 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[2, 4, 12]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.320 = TAGAGCTAGGGAAACA-1

using 177 reads

====================================================================================

graph has 82 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[177]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.321 = TAGAGCTAGGGAGTAA-1

using 49 reads

====================================================================================

graph has 52 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[13, 17, 18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.329 = TAGAGCTAGTTACCCA-1

using 35890 reads

====================================================================================

graph has 9156 edges initially, 112 edges after simplification

total ucounts = 352
nonsolo ucounts = 231[2^51, 3^24, 4^15, 5^12, 6^15, 7^7, 8^7, 9^7, 10^10, 11^7, 12^5, 13^3, 14^7, 15^9, 16, 17^5, 18^4, 19^2, 20, 22, 23^6, 24, 26^2, 27, 28, 30, 32, 34, 37, 38, 40, 41, 42^3, 52, 53, 68, 70, 72, 86^2, 123, 338, 1320, 3359, 3411, 3630, 3969, 4273, 6126, 6772]
surviving nonsolo ucounts = 9[338, 1320, 3359, 3411, 3630, 3969, 4273, 6126, 6772]
ids = [303, 226, 262, 256, 67, 309, 129, 324, 274]

====================================================================================

REJECT CONTIGS

TIG 1[bases=334]
4-140 ==> 595-731 on segment before TRAJ49 exon 1 [len=835] (mis=0)
119-236 ==> 718-835 on segment before TRAJ49 exon 1 [len=835] (mis=0)
236-292 ==> 0-56 on |436|TRAJ49|J-REGION| [len=56] (mis=0)
292-334 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
umis assigned: [309, 324]
of which 2 are surviving nonsolos
reads assigned: 9963
start codons at 42, 118
confident = false
did not find CDR3

TIG 2[bases=315]
5-213 ==> 3018-3226 on segment before TRBJ2-1 exon 1 [len=3226] (mis=0)
213-263 ==> 0-50 on |543|TRBJ2-1|J-REGION| [len=50] (mis=0)
263-315 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
umis assigned: [67, 129, 226, 256, 262, 274, 303]
of which 7 are surviving nonsolos
reads assigned: 21323
start codons at 72, 221
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.331 = TAGAGCTCAAAGGAAG-1

using 39 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[2, 4, 32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.332 = TAGAGCTCAAGAAGAG-1

using 42 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 4[3, 8, 14, 16]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.333 = TAGAGCTCAAGTTCTG-1

using 99 reads

====================================================================================

graph has 76 edges initially, 4 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[11, 12, 34, 40]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.336 = TAGAGCTCACATCCAA-1

using 127 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[127]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.338 = TAGAGCTCACCAGATT-1

using 67 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[67]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.340 = TAGAGCTCACTATCTT-1

using 47 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[47]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.341 = TAGAGCTCAGCCTGTG-1

using 68 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[68]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.347 = TAGAGCTCATAAAGGT-1

using 54 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[3^2, 46]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.350 = TAGAGCTCATCACGTA-1

using 31 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.351 = TAGAGCTCATCATCCC-1

using 109 reads

====================================================================================

graph has 52 edges initially, 4 edges after simplification

total ucounts = 8
nonsolo ucounts = 5[2, 3, 7, 21, 73]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.353 = TAGAGCTCATCGATGT-1

using 77 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[77]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.354 = TAGAGCTCATCGGAAG-1

using 19 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.360 = TAGAGCTGTAAGGGCT-1

using 49 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[3, 5, 40]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.362 = TAGAGCTGTACATGTC-1

using 54 reads

====================================================================================

graph has 48 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 1[52]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.365 = TAGAGCTGTATGAATG-1

using 32 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[14, 18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.366 = TAGAGCTGTCAAAGCG-1

using 29 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[2, 27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.367 = TAGAGCTGTCATACTG-1

using 61632 reads

====================================================================================

graph has 15683 edges initially, 146 edges after simplification

total ucounts = 531
nonsolo ucounts = 372[2^40, 3^33, 4^21, 5^18, 6^19, 7^12, 8^15, 9^12, 10^16, 11^21, 12^8, 13^9, 14^9, 15^3, 16^11, 17^9, 18^7, 19^10, 20^5, 21^4, 22^4, 23^3, 24^2, 25^3, 26^2, 27^3, 28, 29^4, 30, 31^3, 32^2, 35, 36, 37, 39, 40^2, 41, 42, 44, 46, 51, 55, 61, 62, 71, 80^2, 81, 82, 108, 127, 165, 336, 460, 467, 469, 503, 531, 685, 769, 778, 924, 999, 1010, 1092, 1194, 1277, 1287, 1386, 1520, 1525, 1567, 1570, 1618, 1691, 1726, 1727, 1745, 1753, 1758, 1759, 1818, 1900, 1941, 1965, 1986, 1992, 2023, 2027, 2177, 2280, 2688]
surviving nonsolo ucounts = 39[460, 467, 469, 503, 531, 685, 769, 778, 924, 999, 1010, 1092, 1194, 1277, 1287, 1386, 1520, 1525, 1567, 1570, 1618, 1691, 1726, 1727, 1745, 1753, 1758, 1759, 1818, 1900, 1941, 1965, 1986, 1992, 2023, 2027, 2177, 2280, 2688]
ids = [528, 142, 344, 342, 228, 20, 268, 29, 115, 12, ...]

====================================================================================

UMI info for barcode TAGAGCTGTCATACTG-1 contig 1 = ACAGTGATCC...
umi AACTCGTTAC = 999 reads: +409 validated
umi AAGCGGTTAA = 680 reads: +409 validated
umi AAGGAAACCA = 1716 reads: +409 validated
umi AATCATAAGT = 784 reads: +409 validated
umi ATATTATATC = 1389 reads: +409 validated
umi ATGGTAGTAA = 1781 reads: +409 validated
umi ATGTAATTGT = 1772 reads: +409 validated
umi ATTTGTACGT = 1013 reads: +409 validated
umi ATTTGTTCGT = 1582 reads: +409 validated
umi CATCGTACCT = 465 reads: +409 validated
umi CATGAATCCG = 1820 reads: +409 validated
umi CCCCTTCCTC = 1616 reads: +409 validated
umi CCTCTTGGCC = 1901 reads: +409 validated
umi CGATAAGGCA = 2026 reads: +409 validated
umi CGCGCTGTGA = 1522 reads: +409 validated
umi CGGTAAGATC = 1987 reads: +409 validated
umi CTAAGGGCAA = 1753 reads: +409 validated
umi CTACGCCCTT = 1283 reads: +409 validated
umi CTATCATCTA = 463 reads: -364X +45 invalidated
umi CTTACCGTAC = 2181 reads: +409 validated
umi CTTATAATGC = 764 reads: +409 validated
umi GAACGGACCT = 1743 reads: +409 validated
umi GACACTCGCG = 2030 reads: +409 validated
umi GGTACGCTAC = 502 reads: +409 validated
umi GTAGCCTAGC = 2289 reads: +409 validated
umi GTCATACTGT = 1564 reads: +409 validated
umi TAGCCTTCTT = 1740 reads: +409 validated
umi TATATGGTCT = 2007 reads: +409 validated
umi TCTCCAGACC = 2700 reads: +409 validated
umi TCTTTCGTTC = 1692 reads: +409 validated
umi TGCTCTTTTG = 1527 reads: +409 validated
umi TTAGTAGCAT = 1970 reads: +409 validated
umi TTTGCTGGGA = 463 reads: +409 validated

UMI info for barcode TAGAGCTGTCATACTG-1 contig 2 = GAGAAGCCTC...
umi ATTTCCTCGA = 922 reads: +406 validated
umi CCATATAATA = 1194 reads: +406 validated
umi GCGACGCTGC = 1113 reads: +406 validated
umi TAACTTCATC = 1281 reads: +406 validated

GOOD CONTIGS

TIG 1[bases=508]
0-47 ==> 0-47 on |674|TRBV7-2|5'UTR| [len=47] (mis=0)
47-394 ==> 0-347 on |675|TRBV7-2|L-REGION+V-REGION| [len=347] (mis=6)
392-408 ==> 0-16 on |730|TRBD2|D-REGION| [len=16] (mis=3)
406-456 ==> 0-50 on |543|TRBJ2-1|J-REGION| [len=50] (mis=5)
456-508 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 32 umis using 2766 reads
cdr3 = CASSSETSGSFRNEQFF at 377, score = 8 + 8
umis assigned: [12, 20, 21, 29, 82, 100, 103, 116, 118, 142] and 23 others
of which 33 are surviving nonsolos
reads assigned: 48824
start codons at 47, 156, 414
confident = true

TIG 2[bases=565]
0-117 ==> 0-117 on |474|TRAV19|5'UTR| [len=117] (mis=1)
117-467 ==> 0-350 on |475|TRAV19|L-REGION+V-REGION| [len=350] (mis=1)
469-523 ==> 8-62 on |428|TRAJ41|J-REGION| [len=62] (mis=0)
523-565 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 4 umis using 202 reads
cdr3 = CALSETDSGYALNF at 450, score = 7 + 7
umis assigned: [115, 161, 311, 386]
of which 4 are surviving nonsolos
reads assigned: 4433
start codons at 111, 117, 174, 229, 250, 340, 349, 478
confident = true

REJECT CONTIGS

TIG 1[bases=540]
0-105 ==> 0-105 on |459|TRAV12-2|5'UTR| [len=105] (mis=0)
105-439 ==> 0-334 on |460|TRAV12-2|L-REGION+V-REGION| [len=340] (mis=0)
443-498 ==> 6-61 on |427|TRAJ40|J-REGION| [len=61] (mis=0)
498-540 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
cdr3 = CRRSGTYKYIF at 434, score = 3 + 8
umis assigned: [344, 432]
of which 2 are surviving nonsolos
reads assigned: 2349
start codons at 16, 102, 105, 309, 325, 340, 379
confident = false
frameshifted full length stopped transcript of length 540
VJ delta = 21
not full
not full
now this is a cell
paired!

CAAGTCGTGGACTCAGCAGTATACTTCTGTGCTCTGAGTGAGACTGATTCCGGGTATGCACTCAACTTCGGCAAAGGCACCTCGCTGTTGGTCACACCCC <==> GAGGACTCGGCCGTGTATCTCTGTGCCAGCAGCTCTGAGACTAGCGGGAGTTTCCGGAATGAGCAGTTCTTCGGGCCAGGGACACGGCTCACCGTGCTAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.369 = TAGAGCTGTCATCCCT-1

using 39 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[39]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.376 = TAGAGCTGTGCCTGGT-1

using 58 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 5[2^2, 3^2, 46]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.383 = TAGAGCTGTTCACCTC-1

using 30 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.387 = TAGAGCTGTTGATTCG-1

using 22 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.392 = TAGAGCTTCACATACG-1

using 375 reads

====================================================================================

graph has 216 edges initially, 6 edges after simplification

total ucounts = 23
nonsolo ucounts = 16[2^2, 3^2, 6^3, 7, 8, 9, 10, 11, 15, 79, 82, 119]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.400 = TAGAGCTTCCAATGGT-1

using 65 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[65]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.403 = TAGAGCTTCCGGGTGT-1

using 360 reads

====================================================================================

graph has 312 edges initially, 14 edges after simplification

total ucounts = 11
nonsolo ucounts = 9[4, 7, 14, 18, 36, 39, 61, 67, 112]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.405 = TAGAGCTTCGCGTTTC-1

using 46 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[46]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.409 = TAGAGCTTCTAGAGTC-1

using 61 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 4[4, 5, 8, 43]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.414 = TAGAGCTTCTTGACGA-1

using 279 reads

====================================================================================

graph has 150 edges initially, 8 edges after simplification

total ucounts = 13
nonsolo ucounts = 9[2^2, 4, 12, 17, 33, 61, 71, 73]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.415 = TAGAGCTTCTTGAGGT-1

using 254 reads

====================================================================================

graph has 198 edges initially, 10 edges after simplification

total ucounts = 19
nonsolo ucounts = 13[2, 4, 5, 6, 7^2, 9, 15, 16, 28, 34, 47, 68]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.418 = TAGAGCTTCTTGTTTG-1

using 461 reads

====================================================================================

graph has 240 edges initially, 6 edges after simplification

total ucounts = 36
nonsolo ucounts = 33[2, 3^2, 4^3, 5^3, 7^2, 8^4, 9, 11, 12^3, 14, 15^2, 20^2, 22, 23, 25^2, 26, 37, 38, 41]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.419 = TAGAGCTTCTTTAGGG-1

using 16743 reads

====================================================================================

graph has 8042 edges initially, 218 edges after simplification

total ucounts = 853
nonsolo ucounts = 591[2^98, 3^56, 4^57, 5^41, 6^34, 7^22, 8^27, 9^16, 10^16, 11^22, 12^11, 13^14, 14^15, 15^17, 16^10, 17^7, 18^10, 19^16, 20^8, 21^8, 22^12, 23^5, 24^6, 25^5, 26^6, 28, 29^5, 30^6, 31^3, 32^2, 33^3, 34^4, 35, 36^2, 37^3, 40, 41, 42, 47^2, 48, 50^2, 59, 60, 63, 66, 68^2, 71, 72, 77, 88, 89, 143, 632, 9006]
surviving nonsolo ucounts = 4[11, 143, 632, 9006]
ids = [783, 38, 403, 239]

====================================================================================

REJECT CONTIGS

TIG 1[bases=307]
5-78 ==> 727-800 on segment before TRAJ12 exon 1 [len=800] (mis=0)
201-265 ==> 0-64 on |395|TRAJ10|J-REGION| [len=64] (mis=0)
265-307 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
umis assigned: [239]
of which 1 are surviving nonsolos
reads assigned: 8911
start codons at 24, 33, 80
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.428 = TAGCCGGAGAGTAATC-1

using 61 reads

====================================================================================

graph has 84 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 5[5, 11, 13, 14, 15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.432 = TAGCCGGAGATGCCTT-1

using 227 reads

====================================================================================

graph has 245 edges initially, 6 edges after simplification

total ucounts = 16
nonsolo ucounts = 14[2^3, 4, 5, 6, 9^2, 10, 11, 21, 34, 53, 57]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.438 = TAGCCGGAGCCCTAAT-1

using 167 reads

====================================================================================

graph has 178 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 6[3, 5, 8, 22, 45, 82]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.441 = TAGCCGGAGCCGGTAA-1

using 18 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.443 = TAGCCGGAGCGATTCT-1

using 75 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[75]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.450 = TAGCCGGAGGATGGAA-1

using 8663 reads

====================================================================================

graph has 7555 edges initially, 200 edges after simplification

total ucounts = 1114
nonsolo ucounts = 797[2^150, 3^102, 4^71, 5^61, 6^38, 7^43, 8^33, 9^27, 10^23, 11^18, 12^17, 13^23, 14^16, 15^16, 16^13, 17^15, 18^16, 19^11, 20^8, 21^2, 22^6, 23^5, 24^7, 25^5, 26^3, 27^4, 28^8, 29, 30^5, 31^2, 32^4, 33^2, 35, 36^5, 37^2, 38^3, 39^4, 40, 41, 42, 43, 44, 48, 49, 50, 52^2, 54, 55, 57^2, 59, 60^2, 64^2, 65, 66, 70, 72, 73, 74, 98, 105]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.455 = TAGCCGGAGGGATGGG-1

using 41 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[41]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.458 = TAGCCGGAGGTGCAAC-1

using 46049 reads

====================================================================================

graph has 14790 edges initially, 158 edges after simplification

total ucounts = 722
nonsolo ucounts = 501[2^77, 3^62, 4^50, 5^34, 6^33, 7^20, 8^14, 9^25, 10^14, 11^17, 12^12, 13^14, 14^13, 15^7, 16^8, 17^5, 18^3, 19^7, 20^8, 21^4, 22^2, 23^7, 24^5, 25^3, 26^3, 29^3, 32^2, 33, 34, 36, 38^2, 39, 40^4, 42^3, 43, 44, 45, 46^2, 47^5, 53, 56, 80, 101^2, 304, 347, 400, 451, 493, 723, 907, 1151, 1241, 1254, 1420, 1524, 1539, 1631, 1737, 1947, 1957, 2378, 3314, 7462, 8696]
surviving nonsolo ucounts = 20[304, 347, 400, 451, 723, 907, 1151, 1241, 1254, 1420, 1524, 1539, 1631, 1737, 1947, 1957, 2378, 3314, 7462, 8696]
ids = [610, 428, 535, 591, 94, 543, 589, 647, 476, 38, ...]

====================================================================================

UMI info for barcode TAGCCGGAGGTGCAAC-1 contig 1 = AGCTGTGAGG...
umi AAGTCCCTTT = 1465 reads: +23 -1XX +373 invalidated
umi AGAATAGGAT = 721 reads: +397 validated
umi CAACGTGTGT = 1535 reads: +397 validated
umi CTCTTGGTAC = 2 reads: -394X +1 -2XX invalidated
umi GAAGCTCATT = 1762 reads: +397 validated
umi GGACTACCCT = 1639 reads: +397 validated
umi GGTATTATTC = 6785 reads: -375 +6 -1XX +1 -1XX +2 -1XX +5 -2XX +1 -1XX +1 invalidated
umi GTGATATCTT = 6155 reads: -375 +6 -1XX +1 -1XX +2 -1XX +5 -2XX +1 -1XX +1 invalidated
umi GTGTGTAGTT = 1243 reads: +397 validated
umi TAGTGATTGC = 1503 reads: +397 validated
umi TATACGTCCA = 348 reads: -379 +4 -1X +5 -1XX +2 -3XX +2 invalidated
umi TCCGTCAGTT = 1956 reads: +397 validated
umi TCGCAATAGC = 1108 reads: +397 validated
umi TCGGCTTCGT = 469 reads: +160 -3XX +1 -2XX +231 invalidated
umi TGTCTACGGC = 1241 reads: +397 validated

UMI info for barcode TAGCCGGAGGTGCAAC-1 contig 2 = TGGGGAGTGA...
umi CATCGCTTGC = 1 reads: -378 +1 -1X +2 -1X +2 -6X invalidated
umi GGCTAGCGAC = 339 reads: +391 validated
umi TATGATATCG = 766 reads: +367 -1 +23 non-validated
umi TCTCTGCCAT = 3334 reads: +391 validated
umi TGAGCCGTGG = 309 reads: +391 validated
umi TGAGCCGTGT = 2378 reads: +391 validated
umi TGCTGCAGCA = 1993 reads: +30 -4XX +357 invalidated

GOOD CONTIGS

TIG 1[bases=530]
0-81 ==> 0-81 on |585|TRBV18|5'UTR| [len=81] (mis=0)
81-428 ==> 0-347 on |586|TRBV18|L-REGION+V-REGION| [len=347] (mis=3)
430-478 ==> 0-48 on |536|TRBJ1-1|J-REGION| [len=48] (mis=3)
478-530 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 11 umis using 854 reads
cdr3 = CASSADRVTEAFF at 411, score = 7 + 8
umis assigned: [38, 94, 203, 324, 361, 423, 444, 470, 476, 529] and 5 others
of which 14 are surviving nonsolos
reads assigned: 27574
start codons at 66, 81, 139, 150, 203, 213, 229, 273, 304, 315
confident = true

TIG 2[bases=483]
50-390 ==> 0-340 on |503|TRAV36/DV7|L-REGION+V-REGION| [len=340] (mis=3)
385-441 ==> 0-56 on |436|TRAJ49|J-REGION| [len=56] (mis=2)
441-483 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 6 umis using 521 reads
cdr3 = CAVVTGNQFYF at 377, score = 7 + 7
umis assigned: [232, 428, 543, 605, 610, 611, 632]
of which 6 are surviving nonsolos
reads assigned: 8895
start codons at 50, 53, 186, 214, 254
confident = true
now this is a cell
paired!

ACAGCCACCCAGACCGGAGACTCGGCCATCTACCTCTGTGCTGTGGTCACCGGTAACCAGTTCTATTTTGGGACAGGGACAAGTTTGACGGTCATTCCAA <==> CAGGTAGTGCGAGGAGATTCGGCAGCTTATTTCTGTGCCAGCTCAGCAGACAGGGTCACTGAAGCTTTCTTTGGACAAGGCACCAGACTCACAGTTGTAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.463 = TAGCCGGAGTACTTGC-1

using 41 reads

====================================================================================

graph has 56 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 7[2, 4^2, 5, 7, 8, 10]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.466 = TAGCCGGAGTCGTTTG-1

using 18 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.470 = TAGCCGGAGTTAGGTA-1

using 6910 reads

====================================================================================

graph has 6318 edges initially, 192 edges after simplification

total ucounts = 870
nonsolo ucounts = 632[2^103, 3^66, 4^56, 5^46, 6^36, 7^27, 8^24, 9^33, 10^22, 11^27, 12^23, 13^11, 14^19, 15^17, 16^12, 17^9, 18^11, 19^11, 20^6, 21^11, 22^5, 23^5, 24^2, 25^4, 26^4, 27^5, 28^2, 29, 30^2, 31^3, 32^2, 34, 35^4, 36^2, 37^2, 40^2, 41^2, 46, 47^2, 48, 51, 59, 65, 67, 79^2, 84, 92^2, 107]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.472 = TAGCCGGAGTTCGATC-1

using 75 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[75]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.473 = TAGCCGGAGTTGAGTA-1

using 39 reads

====================================================================================

graph has 60 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 6[2^3, 6^2, 18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.475 = TAGCCGGAGTTTCCTT-1

using 98 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[98]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.477 = TAGCCGGCAAATCCGT-1

using 181 reads

====================================================================================

graph has 188 edges initially, 4 edges after simplification

total ucounts = 6
nonsolo ucounts = 5[2, 14, 22, 48, 94]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.481 = TAGCCGGCAAGCTGAG-1

using 105 reads

====================================================================================

graph has 128 edges initially, 8 edges after simplification

total ucounts = 12
nonsolo ucounts = 10[2^3, 3, 4, 10, 12, 19, 20, 29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.482 = TAGCCGGCAATAGAGT-1

using 19 reads

====================================================================================

graph has 6 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.483 = TAGCCGGCACAAGTAA-1

using 1070 reads

====================================================================================

graph has 296 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[6, 1063]
surviving nonsolo ucounts = 1[1063]
ids = [2]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.485 = TAGCCGGCACAGGAGT-1

using 22 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.487 = TAGCCGGCACCAGGTC-1

using 92 reads

====================================================================================

graph has 72 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 4[3, 4, 6, 79]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.491 = TAGCCGGCACGCCAGT-1

using 86 reads

====================================================================================

graph has 54 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[86]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.492 = TAGCCGGCACGCTTTC-1

using 68 reads

====================================================================================

graph has 24 edges initially, 4 edges after simplification

total ucounts = 3
nonsolo ucounts = 3[7, 25, 36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.499 = TAGCCGGCACTTCGAA-1

using 113 reads

====================================================================================

graph has 96 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 5[2^2, 3, 5, 101]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.503 = TAGCCGGCAGATGAGC-1

using 23 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[9, 14]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.504 = TAGCCGGCAGATGGGT-1

using 60 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[60]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.507 = TAGCCGGCAGCTGCTG-1

using 39 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[39]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.510 = TAGCCGGCAGTATCTG-1

using 24 reads

====================================================================================

graph has 6 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.513 = TAGCCGGCATACGCTA-1

using 110 reads

====================================================================================

graph has 66 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[110]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.514 = TAGCCGGCATCACGAT-1

using 31 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 2[2, 26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.518 = TAGCCGGCATCGGTTA-1

using 21 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.519 = TAGCCGGCATGCATGT-1

using 88 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[88]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.521 = TAGCCGGCATGGTCTA-1

using 70 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[70]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.528 = TAGCCGGGTACCGAGA-1

using 89 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[89]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.532 = TAGCCGGGTAGGAGTC-1

using 109 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[108]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.533 = TAGCCGGGTAGGGTAC-1

using 60 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[60]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.535 = TAGCCGGGTCAAAGCG-1

using 490 reads

====================================================================================

graph has 342 edges initially, 14 edges after simplification

total ucounts = 31
nonsolo ucounts = 27[2^5, 3^2, 4^3, 5, 6^2, 7, 8, 12, 13, 14^2, 21, 23, 31^2, 47, 72, 73, 75]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.537 = TAGCCGGGTCAGAGGT-1

using 18 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.538 = TAGCCGGGTCAGTGGA-1

using 148 reads

====================================================================================

graph has 116 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[147]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.540 = TAGCCGGGTCATCGGC-1

using 12 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[2, 10]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.542 = TAGCCGGGTCCAGTAT-1

using 42 reads

====================================================================================

graph has 74 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 4[2, 3, 4, 33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.546 = TAGCCGGGTCGCATAT-1

using 40 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[40]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.548 = TAGCCGGGTCGGATCC-1

using 20 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.550 = TAGCCGGGTCTAGAGG-1

using 76 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[76]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.553 = TAGCCGGGTCTCTTAT-1

using 75 reads

====================================================================================

graph has 28 edges initially, 4 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[30, 45]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.555 = TAGCCGGGTCTGCCAG-1

using 25 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.558 = TAGCCGGGTGGACGAT-1

using 70 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[70]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.566 = TAGCCGGGTTCCAACA-1

using 37 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.570 = TAGCCGGGTTCCTCCA-1

using 44 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[10, 34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.573 = TAGCCGGGTTCTGTTT-1

using 29 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.575 = TAGCCGGGTTGTGGCC-1

using 47 reads

====================================================================================

graph has 58 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 4[3, 4, 13, 23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.582 = TAGCCGGTCAGCATGT-1

using 38 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.584 = TAGCCGGTCAGTGTTG-1

using 44 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[44]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.587 = TAGCCGGTCATCATTC-1

using 48 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[48]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.590 = TAGCCGGTCATTGCCC-1

using 65 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[5, 59]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.591 = TAGCCGGTCCACGAAT-1

using 238 reads

====================================================================================

graph has 104 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[238]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.592 = TAGCCGGTCCACGTGG-1

using 2092 reads

====================================================================================

graph has 846 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 2[23, 2060]
surviving nonsolo ucounts = 1[2060]
ids = [5]

====================================================================================

UMI info for barcode TAGCCGGTCCACGTGG-1 contig 1 = ACAGTGATCC...
umi TGCAACGGCG = 2064 reads: +400 validated

GOOD CONTIGS

TIG 1[bases=499]
0-47 ==> 0-47 on |674|TRBV7-2|5'UTR| [len=47] (mis=0)
47-394 ==> 0-347 on |675|TRBV7-2|L-REGION+V-REGION| [len=347] (mis=5)
400-447 ==> 0-47 on |550|TRBJ2-7|J-REGION| [len=47] (mis=3)
447-499 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 1 umis using 106 reads
cdr3 = CASSRNRENYEQYF at 377, score = 8 + 8
umis assigned: [5]
of which 1 are surviving nonsolos
reads assigned: 2023
start codons at 47, 156
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.599 = TAGCCGGTCCGCTGTT-1

using 85 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[85]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.607 = TAGCCGGTCGGCATCG-1

using 265 reads

====================================================================================

graph has 58 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[265]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.613 = TAGCCGGTCTAACTCT-1

using 88 reads

====================================================================================

graph has 96 edges initially, 4 edges after simplification

total ucounts = 15
nonsolo ucounts = 10[2, 3^3, 4^2, 7, 14, 17, 26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.617 = TAGCCGGTCTAGAGTC-1

using 274 reads

====================================================================================

graph has 285 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[274]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.619 = TAGCCGGTCTCCTATA-1

using 54 reads

====================================================================================

graph has 64 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 4[3, 4^2, 39]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.620 = TAGCCGGTCTCGTATT-1

using 35 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 3[3, 4, 21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.621 = TAGCCGGTCTGACCTC-1

using 155 reads

====================================================================================

graph has 84 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[155]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.624 = TAGCCGGTCTTACCTA-1

using 44 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[44]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.626 = TAGCCGGTCTTCGGTC-1

using 102 reads

====================================================================================

graph has 128 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 8[2^2, 3, 10, 11, 14^2, 43]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.627 = TAGCCGGTCTTGACGA-1

using 57148 reads

====================================================================================

graph has 23846 edges initially, 682 edges after simplification

total ucounts = 1178
nonsolo ucounts = 1001[2^84, 3^70, 4^56, 5^42, 6^22, 7^25, 8^29, 9^25, 10^15, 11^31, 12^19, 13^13, 14^14, 15^12, 16^19, 17^8, 18^10, 19^8, 20^10, 21^12, 22^9, 23^11, 24^12, 25^6, 26^10, 27^6, 28^10, 29^9, 30^7, 31^10, 32^10, 33^11, 34^4, 35^7, 36^4, 37^6, 38^9, 39^12, 40^5, 41^2, 42^5, 43^6, 44^5, 45^11, 46^5, 47^8, 48^7, 49^6, 50^3, 51^4, 52^5, 53^9, 54^5, 55^3, 56^3, 57^3, 58^4, 59^8, 60^5, 61^4, 62^6, 63^3, 64^7, 65^3, 66^4, 67^2, 68^4, 69^3, 70^4, 71^6, 72^5, 73^3, 74^6, 75^2, 76, 77^5, 78^4, 79, 80^3, 81^7, 82^6, 83^5, 84^3, 85^6, 86, 87^2, 88, 89^5, 90, 91^5, 92^6, 93^7, 94, 95^4, 96^4, 97^5, 98^3, 99^5, 100, 101^2, 102^4, 103^4, 104, 105^6, 106^3, 107, 109^2, 110^2, 111, 112, 114, 117, 118, 119^2, 120^3, 121, 123^2, 124, 128^2, 134, 135, 136^3, 138, 139, 142, 155, 160, 161, 165, 2181, 2307, 2596, 7535, 9528]
surviving nonsolo ucounts = 5[2181, 2307, 2596, 7535, 9528]
ids = [694, 50, 813, 842, 719]

====================================================================================

UMI info for barcode TAGCCGGTCTTGACGA-1 contig 1 = GGAGGCAGTG...
umi ACACAATTTA = 2311 reads: +388 validated
umi GCTCTAATCT = 2188 reads: +388 validated
umi GGCCCTTCAT = 9823 reads: +131 -2XX +1 -1XX +2 -9XX +1 -134XX +2 -9XX +1 -4XX +4 -1XX +1 -7XX +78 invalidated
umi GTTGCTCAGA = 2593 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=495]
0-55 ==> 23-78 on |597|TRBV20-1|5'UTR| [len=78] (mis=0)
55-390 ==> 0-335 on |598|TRBV20-1|L-REGION+V-REGION| [len=335] (mis=4)
396-443 ==> 0-47 on |550|TRBJ2-7|J-REGION| [len=47] (mis=4)
443-495 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 3 umis using 446 reads
cdr3 = CSADGRGRYEQYF at 376, score = 7 + 8
umis assigned: [50, 694, 719, 813]
of which 4 are surviving nonsolos
reads assigned: 16375
start codons at 55, 193, 229, 235, 248, 308
confident = true

REJECT CONTIGS

TIG 1[bases=303]
4-199 ==> 5805-6000 on segment before TRBD1 exon 1 [len=6000] (mis=0)
211-251 ==> 8-48 on |537|TRBJ1-2|J-REGION| [len=48] (mis=0)
251-303 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
umis assigned: [254, 281, 842, 932]
of which 1 are surviving nonsolos
reads assigned: 7434
start codons at 76, 86, 160
confident = false
did not find CDR3
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.629 = TAGGCATAGAAACCGC-1

using 64 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[64]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.634 = TAGGCATAGATCGGGT-1

using 85 reads

====================================================================================

graph has 78 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 7[2^2, 6^3, 10, 50]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.636 = TAGGCATAGATGTAAC-1

using 52 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 3[3, 4, 42]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.641 = TAGGCATAGCCACGCT-1

using 49 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[49]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.643 = TAGGCATAGCCCAACC-1

using 26389 reads

====================================================================================

graph has 10101 edges initially, 150 edges after simplification

total ucounts = 802
nonsolo ucounts = 571[2^107, 3^68, 4^48, 5^34, 6^33, 7^29, 8^25, 9^15, 10^26, 11^21, 12^15, 13^10, 14^10, 15^10, 16^9, 17^8, 18^8, 19^3, 20^5, 21^7, 22^6, 23^6, 24^7, 25^7, 26^3, 27^5, 28^4, 29^2, 30, 31, 32^2, 33^3, 34, 37, 39^3, 41, 44^3, 49, 50, 53, 57, 58, 59, 61^2, 66^3, 70, 85, 652, 1000, 1156, 1159, 1643, 1939, 2370, 2456, 2516, 2639, 2911]
surviving nonsolo ucounts = 11[652, 1000, 1156, 1159, 1643, 1939, 2370, 2456, 2516, 2639, 2911]
ids = [717, 372, 219, 201, 387, 342, 490, 437, 76, 800, ...]

====================================================================================

UMI info for barcode TAGGCATAGCCCAACC-1 contig 1 = ACAGTGATCC...
umi ACAGTATTCA = 2530 reads: +406 validated
umi ACGTTCCATT = 2910 reads: +406 validated
umi ATGTGCACCT = 1169 reads: +406 validated
umi ATTTTCTGGA = 1156 reads: +406 validated
umi CGCGACCGTT = 1931 reads: +406 validated
umi CTCAGGCCCC = 997 reads: +406 validated
umi CTCTTTCCGT = 1656 reads: +406 validated
umi GAGTAACAAG = 2463 reads: +406 validated
umi GGAAGGTCAA = 2394 reads: +406 validated
umi TTAAGTCCAG = 657 reads: +406 validated
umi TTTTTTGTTC = 2877 reads: +146 -2XX +2 -1XX +255 invalidated

GOOD CONTIGS

TIG 1[bases=505]
0-47 ==> 0-47 on |674|TRBV7-2|5'UTR| [len=47] (mis=0)
47-394 ==> 0-347 on |675|TRBV7-2|L-REGION+V-REGION| [len=347] (mis=6)
404-453 ==> 0-49 on |546|TRBJ2-3|J-REGION| [len=49] (mis=2)
453-505 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 11 umis using 1225 reads
cdr3 = CASSLGLAGVTDTQYF at 377, score = 8 + 8
umis assigned: [76, 112, 201, 219, 342, 372, 387, 437, 490, 717] and 1 others
of which 11 are surviving nonsolos
reads assigned: 20137
start codons at 47, 156
confident = true
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.649 = TAGGCATAGCTGCCCA-1

using 74 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[74]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.652 = TAGGCATAGGACACCA-1

using 79 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[79]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.657 = TAGGCATAGGCATTGG-1

using 52 reads

====================================================================================

graph has 60 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 5[2, 3^2, 12, 31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.660 = TAGGCATAGGGAGTAA-1

using 63 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 5[2, 13, 14, 16^2]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.663 = TAGGCATAGTACGATA-1

using 81 reads

====================================================================================

graph has 52 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[8, 9, 62]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.669 = TAGGCATAGTCGCCGT-1

using 413 reads

====================================================================================

graph has 86 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 2[3, 407]
surviving nonsolo ucounts = 1[407]
ids = [2]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.670 = TAGGCATAGTGGGATC-1

using 1666 reads

====================================================================================

graph has 736 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 4[2, 3, 20, 1637]
surviving nonsolo ucounts = 1[1637]
ids = [5]

====================================================================================

UMI info for barcode TAGGCATAGTGGGATC-1 contig 1 = AGAGCTGGAA...
umi TATAACTTAG = 1646 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=476]
0-36 ==> 0-36 on |604|TRBV24-1|5'UTR| [len=36] (mis=0)
36-381 ==> 0-345 on |605|TRBV24-1|L-REGION+V-REGION| [len=345] (mis=4)
382-424 ==> 8-50 on |543|TRBJ2-1|J-REGION| [len=50] (mis=0)
424-476 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 1 umis using 98 reads
cdr3 = CATSVDHEQFF at 363, score = 8 + 8
umis assigned: [5]
of which 1 are surviving nonsolos
reads assigned: 1607
start codons at 31, 36, 90, 94, 100, 150, 158, 178, 186, 244, 277, 382
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.672 = TAGGCATAGTGTCTCA-1

using 82 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[82]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.678 = TAGGCATCAAAGTCAA-1

using 48 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[48]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.679 = TAGGCATCAAATACAG-1

using 217 reads

====================================================================================

graph has 164 edges initially, 4 edges after simplification

total ucounts = 22
nonsolo ucounts = 16[2^2, 3^2, 4, 5^2, 6^2, 7, 8^2, 9, 11, 52, 80]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.688 = TAGGCATCAATTGCTG-1

using 84 reads

====================================================================================

graph has 90 edges initially, 4 edges after simplification

total ucounts = 7
nonsolo ucounts = 6[2, 3^2, 13, 30, 32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.691 = TAGGCATCACAGGCCT-1

using 62 reads

====================================================================================

graph has 32 edges initially, 4 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[17, 45]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.698 = TAGGCATCAGAAGCAC-1

using 106 reads

====================================================================================

graph has 66 edges initially, 6 edges after simplification

total ucounts = 9
nonsolo ucounts = 6[2, 4, 7, 17, 23, 50]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.699 = TAGGCATCAGACACTT-1

using 90 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[90]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.701 = TAGGCATCAGAGTGTG-1

using 19 reads

====================================================================================

graph has 6 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.704 = TAGGCATCAGCGATCC-1

using 79 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[79]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.708 = TAGGCATCAGGACCCT-1

using 20 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.709 = TAGGCATCAGGATCGA-1

using 61 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 4[2^3, 54]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.712 = TAGGCATCAGGTGGAT-1

using 68 reads

====================================================================================

graph has 78 edges initially, 2 edges after simplification

total ucounts = 12
nonsolo ucounts = 7[2^3, 4, 10, 11, 32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.714 = TAGGCATCATACAGCT-1

using 55 reads

====================================================================================

graph has 68 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 6[2, 4, 5, 6^2, 29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.717 = TAGGCATCATAGGATA-1

using 135 reads

====================================================================================

graph has 72 edges initially, 4 edges after simplification

total ucounts = 10
nonsolo ucounts = 7[2, 4, 14, 16, 19, 30, 47]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.719 = TAGGCATCATCACGAT-1

using 327 reads

====================================================================================

graph has 256 edges initially, 10 edges after simplification

total ucounts = 9
nonsolo ucounts = 6[2, 37, 64, 66, 77, 78]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.720 = TAGGCATCATCACGTA-1

using 28 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.721 = TAGGCATCATCATCCC-1

using 29 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.722 = TAGGCATCATCCAACA-1

using 34 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.725 = TAGGCATCATCGGAAG-1

using 15 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.726 = TAGGCATCATCGGACC-1

using 14 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[14]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.727 = TAGGCATCATGAGCGA-1

using 42 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 3[3, 15, 24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.733 = TAGGCATCATTCTCAT-1

using 93 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[93]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.734 = TAGGCATCATTGAGCT-1

using 12 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[12]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.738 = TAGGCATGTAAGTTCC-1

using 14665 reads

====================================================================================

graph has 12728 edges initially, 494 edges after simplification

total ucounts = 1436
nonsolo ucounts = 1063[2^142, 3^102, 4^63, 5^48, 6^50, 7^57, 8^58, 9^36, 10^35, 11^32, 12^32, 13^26, 14^28, 15^27, 16^14, 17^25, 18^24, 19^25, 20^13, 21^14, 22^18, 23^15, 24^16, 25^21, 26^13, 27^13, 28^14, 29^7, 30^6, 31^10, 32^3, 33^4, 34^7, 35^7, 36^5, 37, 38, 39^6, 40, 41, 42^2, 43^2, 44^4, 45^2, 46^2, 47^2, 49, 51^2, 52, 54^3, 56, 57, 58, 60, 61, 62, 64, 66, 67, 68, 69, 72, 75^2, 78, 81, 90, 95, 101, 103, 115, 127]
surviving nonsolo ucounts = 2[9, 39]
ids = [1282, 370]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.742 = TAGGCATGTAGGGACT-1

using 22 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.745 = TAGGCATGTCAACATC-1

using 60 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[60]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.748 = TAGGCATGTCATATCG-1

using 52 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 4[2, 3, 21, 23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.749 = TAGGCATGTCCAGTAT-1

using 102 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[102]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.752 = TAGGCATGTCCTCTTG-1

using 66 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[66]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.754 = TAGGCATGTCGGCACT-1

using 46 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[46]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.755 = TAGGCATGTCGGCATC-1

using 71 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[35, 36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.756 = TAGGCATGTCTAGTCA-1

using 40 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 1[35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.758 = TAGGCATGTGACTCAT-1

using 59 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[3, 15, 40]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.759 = TAGGCATGTGCAGGTA-1

using 58 reads

====================================================================================

graph has 58 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 3[4, 9, 45]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.762 = TAGGCATGTGCTAGCC-1

using 48 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[48]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.765 = TAGGCATGTGTAAGTA-1

using 48 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 1[43]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.767 = TAGGCATGTGTGCCTG-1

using 19 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.773 = TAGGCATGTTCCTCCA-1

using 1897 reads

====================================================================================

graph has 702 edges initially, 4 edges after simplification

total ucounts = 7
nonsolo ucounts = 6[7, 8^2, 10, 19, 1844]
surviving nonsolo ucounts = 1[1844]
ids = [6]

====================================================================================

UMI info for barcode TAGGCATGTTCCTCCA-1 contig 1 = AATGCACAGA...
umi CTGTTATCCT = 1865 reads: +397 validated

GOOD CONTIGS

TIG 1[bases=492]
0-43 ==> 23-66 on |657|TRBV6-5|5'UTR| [len=66] (mis=0)
43-387 ==> 0-344 on |658|TRBV6-5|L-REGION+V-REGION| [len=344] (mis=4)
381-393 ==> 0-12 on |535|TRBD1|D-REGION| [len=12] (mis=3)
392-440 ==> 0-48 on |536|TRBJ1-1|J-REGION| [len=48] (mis=4)
440-492 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 1 umis using 117 reads
cdr3 = CASSKQGARTEAFF at 370, score = 9 + 8
umis assigned: [6]
of which 1 are surviving nonsolos
reads assigned: 1812
start codons at 1, 29, 43, 101, 154, 178, 185, 193, 220, 284, 293
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.776 = TAGGCATTCAAGCCTA-1

using 73 reads

====================================================================================

graph has 74 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 5[3, 10, 13, 20, 27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.779 = TAGGCATTCACAGTAC-1

using 37 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.781 = TAGGCATTCACTATTC-1

using 32 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.784 = TAGGCATTCATAGCAC-1

using 255 reads

====================================================================================

graph has 495 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[254]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.785 = TAGGCATTCATCACCC-1

using 2720 reads

====================================================================================

graph has 800 edges initially, 4 edges after simplification

total ucounts = 8
nonsolo ucounts = 3[33, 34, 2648]
surviving nonsolo ucounts = 1[2648]
ids = [5]

====================================================================================

UMI info for barcode TAGGCATTCATCACCC-1 contig 1 = AGTCTGCCCA...
umi GGCAGCCTCC = 2666 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=497]
0-51 ==> 17-68 on |552|TRBV10-1|5'UTR| [len=68] (mis=0)
51-389 ==> 0-338 on |553|TRBV10-1|L-REGION+V-REGION| [len=344] (mis=1)
405-445 ==> 7-47 on |550|TRBJ2-7|J-REGION| [len=47] (mis=0)
445-497 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 1 umis using 178 reads
cdr3 = CASSSNPAREQYF at 378, score = 9 + 8
umis assigned: [5]
of which 1 are surviving nonsolos
reads assigned: 2617
start codons at 51, 73, 109, 201, 229, 256, 292
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.789 = TAGGCATTCATGTGGT-1

using 48 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[48]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.795 = TAGGCATTCCGCAGTG-1

using 65 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[65]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.796 = TAGGCATTCCTAGGGC-1

using 42 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 7[2^3, 3, 6, 7, 17]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.802 = TAGGCATTCGATAGAA-1

using 85 reads

====================================================================================

graph has 56 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[85]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.804 = TAGGCATTCGGAGCAA-1

using 218 reads

====================================================================================

graph has 80 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 5[3^3, 9, 199]
surviving nonsolo ucounts = 1[199]
ids = [3]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.807 = TAGGCATTCGTGGGAA-1

using 109 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[109]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.811 = TAGGCATTCTCTTATG-1

using 122 reads

====================================================================================

graph has 170 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 6[3, 4, 5, 10, 16, 80]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.813 = TAGGCATTCTTCGGTC-1

using 33 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 5[2, 3^2, 5, 15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.816 = TAGGCATTCTTGCCGT-1

using 2098 reads

====================================================================================

graph has 814 edges initially, 4 edges after simplification

total ucounts = 10
nonsolo ucounts = 7[4^3, 7, 12, 55, 2009]
surviving nonsolo ucounts = 1[2009]
ids = [8]

====================================================================================

REJECT CONTIGS

TIG 1[bases=459]
4-197 ==> 154-347 on |584|TRBV18|L-REGION+V-REGION| [len=347] (mis=2)
194-206 ==> 0-12 on |535|TRBD1|D-REGION| [len=12] (mis=2)
356-407 ==> 0-51 on |539|TRBJ1-4|J-REGION| [len=51] (mis=0)
407-459 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
cdr3 = CASSPRQGARGYTF at 180, score = 7 + 9
umis assigned: [8]
of which 1 are surviving nonsolos
reads assigned: 1980
start codons at 42, 73, 84, 348, 362
confident = false
not full
VJ delta = -137
delta too large
not full
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.820 = TAGTGGTAGACTAAGT-1

using 189 reads

====================================================================================

graph has 182 edges initially, 8 edges after simplification

total ucounts = 24
nonsolo ucounts = 14[2^2, 3^3, 4, 5, 7, 10, 16, 25, 30, 33, 36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.821 = TAGTGGTAGAGACGAA-1

using 2038 reads

====================================================================================

graph has 966 edges initially, 14 edges after simplification

total ucounts = 28
nonsolo ucounts = 15[2^2, 3, 4^3, 7, 9, 11, 19, 22, 23, 40, 53, 1822]
surviving nonsolo ucounts = 1[1822]
ids = [10]

====================================================================================

UMI info for barcode TAGTGGTAGAGACGAA-1 contig 1 = CATGTTCACA...
umi CGGGGCGATG = 1823 reads: +400 validated

GOOD CONTIGS

TIG 1[bases=505]
0-53 ==> 6-59 on |569|TRBV12-4|5'UTR| [len=59] (mis=0)
53-400 ==> 0-347 on |570|TRBV12-4|L-REGION+V-REGION| [len=347] (mis=4)
405-453 ==> 0-48 on |536|TRBJ1-1|J-REGION| [len=48] (mis=0)
453-505 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 1 umis using 130 reads
cdr3 = CASRQAGVNTEAFF at 383, score = 8 + 8
umis assigned: [10]
of which 1 are surviving nonsolos
reads assigned: 1789
start codons at 1, 53, 111, 152, 175, 224, 227, 276, 287, 314, 321
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.822 = TAGTGGTAGAGCTTCT-1

using 276 reads

====================================================================================

graph has 236 edges initially, 6 edges after simplification

total ucounts = 19
nonsolo ucounts = 13[2^2, 5, 6^2, 7^2, 17, 22, 26, 27, 64, 79]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.823 = TAGTGGTAGAGGTAGA-1

using 65 reads

====================================================================================

graph has 80 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[65]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.824 = TAGTGGTAGAGTTGGC-1

using 96 reads

====================================================================================

graph has 121 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 10[2, 3, 4, 5^2, 7, 8, 11, 19, 32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.826 = TAGTGGTAGATCCGAG-1

using 816 reads

====================================================================================

graph has 408 edges initially, 16 edges after simplification

total ucounts = 48
nonsolo ucounts = 36[2^4, 3^2, 4^2, 5^5, 6, 7, 8^2, 10^3, 11, 13, 14, 17, 21, 25, 26, 27, 28, 49, 54, 71, 77, 78, 87, 100]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.827 = TAGTGGTAGATCTGAA-1

using 302 reads

====================================================================================

graph has 284 edges initially, 8 edges after simplification

total ucounts = 20
nonsolo ucounts = 16[2, 4^2, 6^2, 7, 8^2, 16, 17, 21, 24, 25^2, 44, 81]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.828 = TAGTGGTAGATCTGCT-1

using 211 reads

====================================================================================

graph has 226 edges initially, 8 edges after simplification

total ucounts = 23
nonsolo ucounts = 16[2^3, 3^3, 4, 6^2, 9, 12, 14, 15, 35, 43, 45]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.829 = TAGTGGTAGATTACCC-1

using 60 reads

====================================================================================

graph has 78 edges initially, 2 edges after simplification

total ucounts = 12
nonsolo ucounts = 7[3^2, 6, 7, 9^2, 18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.833 = TAGTGGTAGCAGGCTA-1

using 152 reads

====================================================================================

graph has 128 edges initially, 6 edges after simplification

total ucounts = 14
nonsolo ucounts = 10[2, 3, 5, 7^2, 9, 17, 21, 25, 52]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.836 = TAGTGGTAGCGAAGGG-1

using 51 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[51]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.837 = TAGTGGTAGCGCTTAT-1

using 74658 reads

====================================================================================

graph has 26620 edges initially, 543 edges after simplification

total ucounts = 2162
nonsolo ucounts = 1705[2^186, 3^133, 4^111, 5^108, 6^94, 7^93, 8^59, 9^62, 10^56, 11^59, 12^62, 13^54, 14^43, 15^43, 16^45, 17^22, 18^32, 19^35, 20^22, 21^28, 22^17, 23^21, 24^15, 25^9, 26^16, 27^12, 28^10, 29^10, 30^6, 31^5, 32^9, 33^7, 34^7, 35^5, 36^7, 37^4, 38^4, 39^9, 40^6, 41^9, 42^4, 43^2, 44^5, 45^6, 46^5, 47^3, 48^5, 49^6, 50^6, 51^5, 52^4, 53^4, 54^5, 55^2, 56^4, 57^2, 58^7, 59^4, 60^3, 61^3, 62^4, 63^3, 64^2, 65^3, 66^2, 67^5, 68^2, 69^3, 70^2, 71, 73^4, 74, 75^4, 76^2, 77, 80^4, 81^2, 82^2, 83^4, 84, 85, 86, 89, 90^3, 91^2, 95, 96^2, 97, 101, 105, 107, 110, 112, 114, 147, 357, 411, 519, 1330, 1703, 1798, 1800, 1838, 1846, 1926, 2147, 3354, 4023, 24376]
surviving nonsolo ucounts = 13[411, 519, 1330, 1703, 1798, 1800, 1838, 1846, 1926, 2147, 3354, 4023, 24376]
ids = [574, 1756, 485, 1195, 570, 347, 762, 650, 950, 1529, ...]

====================================================================================

UMI info for barcode TAGTGGTAGCGCTTAT-1 contig 1 = TGGGGGCTCA...
umi ATGTAACCTG = 1326 reads: +400 validated
umi CACAGCGGCA = 1884 reads: +122 -5XX +1 -1XX +1 -2XX +1 -2XX +1 -3XX +2 -1XX +1 -2XX +1 -3XX +1 -21X +1 -2X +1 -2XX +2 -7XX +1 -1XX +1 -8XX +1 -1XX +4 -1XX +196 invalidated
umi CACATAAACA = 408 reads: +400 validated
umi CATATGCTAC = 1858 reads: +400 validated
umi CCCGGCTTCT = 1859 reads: +400 validated
umi CGGTATACCG = 1940 reads: +400 validated
umi GGTCACCTAG = 8 reads: -396X +2 -2XX invalidated
umi GTTACGGGCT = 2169 reads: +400 validated
umi TAGAGGCCTT = 4236 reads: +122 -5XX +1 -1XX +1 -2XX +1 -2XX +1 -3XX +2 -1XX +1 -2XX +1 -2XX +2 -27 +2 -7XX +1 -1XX +1 -8XX +1 -1XX +4 -1XX +196 invalidated
umi TCCACTCCGT = 524 reads: +400 validated
umi TGTCGTGCAC = 3509 reads: +122 -5XX +1 -1XX +1 -2XX +1 -2XX +1 -3XX +2 -1XX +1 -2XX +1 -25XX +1 -5X +2 -7XX +1 -1XX +1 -8XX +1 -1XX +4 -1XX +196 invalidated

GOOD CONTIGS

TIG 1[bases=508]
56-403 ==> 0-347 on |688|TRBV7-9|L-REGION+V-REGION| [len=347] (mis=4)
406-456 ==> 0-50 on |543|TRBJ2-1|J-REGION| [len=50] (mis=4)
456-508 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 10 umis using 1319 reads
cdr3 = CASSAGQGHNEQFF at 386, score = 7 + 8
umis assigned: [485, 570, 574, 650, 762, 950, 1432, 1529, 1637, 1756] and 1 others
of which 10 are surviving nonsolos
reads assigned: 18954
start codons at 56, 80, 165, 264, 377, 414
confident = true

REJECT CONTIGS

TIG 1[bases=471]
6-97 ==> 131-222 on segment before TRBJ1-5 exon 1 [len=222] (mis=0)
97-147 ==> 0-50 on |540|TRBJ1-5|J-REGION| [len=50] (mis=0)
440-471 ==> 0-31 on |531|TRBC1|C-REGION| [len=530] (mis=0)
umis assigned: [347]
of which 1 are surviving nonsolos
reads assigned: 1748
start codons at 84, 87, 123, 148, 192, 253, 335, 351, 410
confident = false
did not find CDR3

TIG 2[bases=318]
5-219 ==> 126-340 on |460|TRAV12-2|L-REGION+V-REGION| [len=340] (mis=3)
221-276 ==> 8-63 on |398|TRAJ13|J-REGION| [len=63] (mis=1)
276-318 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
cdr3 = CAVPLGGYQKVTF at 206, score = 9 + 8
umis assigned: [820]
of which 1 are surviving nonsolos
reads assigned: 24036
start codons at 83, 99, 114, 153
confident = false
VJ delta = 25
not full
not full

TIG 3[bases=458]
0-23 ==> 0-23 on |463|TRAV13-1|5'UTR| [len=23] (mis=0)
23-354 ==> 0-331 on |464|TRAV13-1|L-REGION+V-REGION| [len=337] (mis=1)
361-416 ==> 7-62 on |415|TRAJ3|J-REGION| [len=62] (mis=0)
416-458 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
cdr3 = CRPGSSASKIIF at 349, score = 3 + 9
umis assigned: [1195, 1357]
of which 1 are surviving nonsolos
reads assigned: 1642
start codons at 23, 78, 87, 237
confident = false
frameshifted full length stopped transcript of length 458
VJ delta = 22
not full
not full
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.840 = TAGTGGTAGCTTATCG-1

using 17 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[17]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.841 = TAGTGGTAGCTTCGCG-1

using 208 reads

====================================================================================

graph has 148 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[208]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.843 = TAGTGGTAGGAATCGC-1

using 168 reads

====================================================================================

graph has 248 edges initially, 8 edges after simplification

total ucounts = 21
nonsolo ucounts = 10[2^2, 3, 4, 6, 10^3, 47, 63]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.844 = TAGTGGTAGGACATTA-1

using 188 reads

====================================================================================

graph has 233 edges initially, 8 edges after simplification

total ucounts = 18
nonsolo ucounts = 14[2^2, 3^3, 4, 5, 7, 8, 13, 14, 27, 37, 56]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.845 = TAGTGGTAGGACTGGT-1

using 54 reads

====================================================================================

graph has 68 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[54]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.846 = TAGTGGTAGGAGCGAG-1

using 221 reads

====================================================================================

graph has 214 edges initially, 18 edges after simplification

total ucounts = 15
nonsolo ucounts = 15[2^3, 3, 4, 6, 10^2, 11, 13, 18, 23, 26^2, 65]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.847 = TAGTGGTAGGATATAC-1

using 153 reads

====================================================================================

graph has 120 edges initially, 4 edges after simplification

total ucounts = 12
nonsolo ucounts = 10[3^2, 6, 9^2, 11, 20, 22, 24, 44]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.848 = TAGTGGTAGGCACATG-1

using 151 reads

====================================================================================

graph has 162 edges initially, 4 edges after simplification

total ucounts = 14
nonsolo ucounts = 10[3^2, 5, 7, 8, 11, 12, 13, 22, 63]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.849 = TAGTGGTAGGCATTGG-1

using 296 reads

====================================================================================

graph has 263 edges initially, 6 edges after simplification

total ucounts = 23
nonsolo ucounts = 17[2^3, 4^3, 7, 8, 11, 17, 22, 26, 28, 31, 33, 40, 49]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.856 = TAGTGGTAGGTGCTTT-1

using 73 reads

====================================================================================

graph has 50 edges initially, 6 edges after simplification

total ucounts = 10
nonsolo ucounts = 8[2, 3, 5, 8, 9, 12^2, 20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.858 = TAGTGGTAGTACATGA-1

using 18 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.859 = TAGTGGTAGTAGTGCG-1

using 280 reads

====================================================================================

graph has 252 edges initially, 6 edges after simplification

total ucounts = 28
nonsolo ucounts = 23[2^4, 3, 4, 5, 6^3, 7^4, 8, 10, 12, 18, 20, 21, 26, 38, 56]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.860 = TAGTGGTAGTCCAGGA-1

using 85 reads

====================================================================================

graph has 84 edges initially, 4 edges after simplification

total ucounts = 16
nonsolo ucounts = 11[2^3, 3^3, 7, 9, 15, 16, 18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.861 = TAGTGGTAGTCCGTAT-1

using 92 reads

====================================================================================

graph has 114 edges initially, 4 edges after simplification

total ucounts = 10
nonsolo ucounts = 3[12, 18, 55]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.862 = TAGTGGTAGTCCTCCT-1

using 46 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[46]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.863 = TAGTGGTAGTGAACAT-1

using 245 reads

====================================================================================

graph has 206 edges initially, 6 edges after simplification

total ucounts = 23
nonsolo ucounts = 18[2^2, 3^3, 4^2, 5, 12, 14^2, 15, 17, 19^2, 32, 35, 37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.864 = TAGTGGTAGTGAAGTT-1

using 246 reads

====================================================================================

graph has 166 edges initially, 6 edges after simplification

total ucounts = 18
nonsolo ucounts = 13[2^3, 4^2, 6, 10, 15, 27, 29, 30, 50, 60]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.869 = TAGTGGTAGTGTACCT-1

using 352 reads

====================================================================================

graph has 296 edges initially, 10 edges after simplification

total ucounts = 18
nonsolo ucounts = 13[2^3, 7, 9, 10, 16, 23, 24, 37, 52, 61, 102]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.872 = TAGTGGTAGTTGTCGT-1

using 43 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[42]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.874 = TAGTGGTAGTTTCCTT-1

using 69 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[69]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.877 = TAGTGGTCAAGCCGCT-1

using 111 reads

====================================================================================

graph has 72 edges initially, 4 edges after simplification

total ucounts = 12
nonsolo ucounts = 6[4, 7^2, 11, 17, 59]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.878 = TAGTGGTCAAGGTTCT-1

using 59 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[59]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.888 = TAGTGGTCACCAGGTC-1

using 128 reads

====================================================================================

graph has 124 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 8[4^2, 7, 11, 14, 21, 27, 38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.890 = TAGTGGTCACCTCGTT-1

using 69 reads

====================================================================================

graph has 82 edges initially, 4 edges after simplification

total ucounts = 7
nonsolo ucounts = 6[3^2, 4, 8, 22, 28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.892 = TAGTGGTCACGGCTAC-1

using 132 reads

====================================================================================

graph has 92 edges initially, 4 edges after simplification

total ucounts = 7
nonsolo ucounts = 6[2, 3^2, 30, 43, 50]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.893 = TAGTGGTCACGGTAAG-1

using 136 reads

====================================================================================

graph has 128 edges initially, 4 edges after simplification

total ucounts = 9
nonsolo ucounts = 9[2, 3^3, 11^2, 19, 40, 44]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.894 = TAGTGGTCACTAAGTC-1

using 1151 reads

====================================================================================

graph has 562 edges initially, 6 edges after simplification

total ucounts = 11
nonsolo ucounts = 9[2, 6, 7, 8, 10, 13, 14, 18, 1071]
surviving nonsolo ucounts = 1[1071]
ids = [8]

====================================================================================

UMI info for barcode TAGTGGTCACTAAGTC-1 contig 1 = AGCTGTGAGG...
umi TATACTTTCT = 949 reads: +400 validated

GOOD CONTIGS

TIG 1[bases=489]
0-81 ==> 0-81 on |585|TRBV18|5'UTR| [len=81] (mis=0)
81-422 ==> 0-341 on |586|TRBV18|L-REGION+V-REGION| [len=347] (mis=0)
431-481 ==> 0-50 on |540|TRBJ1-5|J-REGION| [len=50] (mis=0)
junction support: 1 umis using 35 reads
cdr3 = CASSEGFSNQPQHF at 411, score = 7 + 8
umis assigned: [8]
of which 1 are surviving nonsolos
reads assigned: 934
start codons at 66, 81, 139, 150, 203, 213, 229, 273, 304, 315, 457
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.896 = TAGTGGTCAGACGCAA-1

using 42 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[42]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.899 = TAGTGGTCAGATGAGC-1

using 16 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[7, 9]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.900 = TAGTGGTCAGATGGCA-1

using 143 reads

====================================================================================

graph has 118 edges initially, 4 edges after simplification

total ucounts = 8
nonsolo ucounts = 5[2, 4, 15, 40, 79]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.901 = TAGTGGTCAGATTGCT-1

using 101 reads

====================================================================================

graph has 48 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[101]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.904 = TAGTGGTCAGCTGTAT-1

using 76 reads

====================================================================================

graph has 76 edges initially, 4 edges after simplification

total ucounts = 9
nonsolo ucounts = 7[3, 4, 6^2, 11, 21, 23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.905 = TAGTGGTCAGCTGTTA-1

using 83 reads

====================================================================================

graph has 90 edges initially, 2 edges after simplification

total ucounts = 16
nonsolo ucounts = 11[2^3, 3^2, 4, 7^2, 8, 9, 31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.909 = TAGTGGTCAGTATCTG-1

using 31 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.910 = TAGTGGTCAGTTTACG-1

using 224 reads

====================================================================================

graph has 205 edges initially, 6 edges after simplification

total ucounts = 20
nonsolo ucounts = 13[2, 3^2, 5, 9^2, 18, 19, 22, 27, 30, 32, 38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.914 = TAGTGGTCATCGACGC-1

using 207 reads

====================================================================================

graph has 172 edges initially, 12 edges after simplification

total ucounts = 10
nonsolo ucounts = 8[2, 6^2, 8, 14, 32, 63, 74]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.919 = TAGTGGTCATTCTTAC-1

using 186 reads

====================================================================================

graph has 104 edges initially, 6 edges after simplification

total ucounts = 13
nonsolo ucounts = 10[2^2, 10^2, 16, 17, 19, 22, 33, 52]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.923 = TAGTGGTGTAAAGTCA-1

using 132 reads

====================================================================================

graph has 169 edges initially, 2 edges after simplification

total ucounts = 13
nonsolo ucounts = 11[2, 4^2, 7^2, 10^2, 14, 15, 20, 37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.928 = TAGTGGTGTACCGTTA-1

using 112 reads

====================================================================================

graph has 106 edges initially, 4 edges after simplification

total ucounts = 5
nonsolo ucounts = 4[11, 15, 35, 50]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.929 = TAGTGGTGTACTCAAC-1

using 36845 reads

====================================================================================

graph has 19105 edges initially, 454 edges after simplification

total ucounts = 1918
nonsolo ucounts = 1484[2^170, 3^99, 4^76, 5^89, 6^87, 7^59, 8^61, 9^60, 10^44, 11^49, 12^47, 13^37, 14^33, 15^39, 16^28, 17^27, 18^26, 19^28, 20^28, 21^20, 22^32, 23^21, 24^28, 25^15, 26^22, 27^24, 28^17, 29^16, 30^26, 31^10, 32^16, 33^8, 34^8, 35^6, 36^11, 37^7, 38^8, 39^6, 40^2, 41^11, 42^3, 43^4, 44^5, 45^6, 46^3, 47^7, 48^2, 49^6, 50^2, 51^4, 52^3, 53^4, 56^3, 57^3, 60^2, 61^2, 62^2, 63^2, 64, 65^2, 66^2, 70, 73, 75, 77, 82, 83, 89, 93, 97, 103, 1312, 1562, 1943, 2745, 6760]
surviving nonsolo ucounts = 5[1312, 1562, 1943, 2745, 6760]
ids = [215, 585, 1247, 638, 355]

====================================================================================

UMI info for barcode TAGTGGTGTACTCAAC-1 contig 1 = ACTACTGGGA...
umi ACCCGTAAGT = 1302 reads: +394 validated
umi AGATCCCGGT = 5657 reads: -321X +1 -2XX +2 -1XX +2 -11XX +1 -1XX +1 -2XX +1 -2XX +1 -3XX +1 -1XX +40 invalidated
umi CAAACACATC = 1536 reads: +394 validated
umi CACCTGTTCC = 2779 reads: +394 validated
umi GCTTTCCTCT = 1949 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=483]
0-37 ==> 0-37 on |606|TRBV25-1|5'UTR| [len=37] (mis=0)
37-381 ==> 0-344 on |607|TRBV25-1|L-REGION+V-REGION| [len=344] (mis=1)
391-431 ==> 8-48 on |537|TRBJ1-2|J-REGION| [len=48] (mis=0)
431-483 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 4 umis using 486 reads
cdr3 = CASSELAGDGYTF at 364, score = 7 + 9
umis assigned: [215, 355, 585, 638, 1247]
of which 5 are surviving nonsolos
reads assigned: 12972
start codons at 37, 61, 91, 159, 172, 179, 187, 214, 242
confident = true
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.930 = TAGTGGTGTAGCACGA-1

using 139 reads

====================================================================================

graph has 142 edges initially, 4 edges after simplification

total ucounts = 14
nonsolo ucounts = 10[4^2, 5, 6, 10, 11, 14, 16, 30, 35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.937 = TAGTGGTGTATAGGGC-1

using 209 reads

====================================================================================

graph has 159 edges initially, 12 edges after simplification

total ucounts = 23
nonsolo ucounts = 15[2^3, 3, 5^2, 6, 10, 14, 19^3, 28, 30, 37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.939 = TAGTGGTGTATATGAG-1

using 34 reads

====================================================================================

graph has 56 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 3[3, 4, 23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.944 = TAGTGGTGTCAGCTAT-1

using 108 reads

====================================================================================

graph has 110 edges initially, 8 edges after simplification

total ucounts = 11
nonsolo ucounts = 8[3, 6, 7, 8, 9, 14, 20, 38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.948 = TAGTGGTGTCATCCCT-1

using 191 reads

====================================================================================

graph has 206 edges initially, 8 edges after simplification

total ucounts = 13
nonsolo ucounts = 11[3, 5, 6, 8, 13, 19, 23^2, 27, 30, 32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.951 = TAGTGGTGTCCGAGTC-1

using 75 reads

====================================================================================

graph has 68 edges initially, 2 edges after simplification

total ucounts = 14
nonsolo ucounts = 9[2, 3, 6, 7^2, 9^3, 18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.952 = TAGTGGTGTCCTGCTT-1

using 43 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[43]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.956 = TAGTGGTGTCGGATCC-1

using 22900 reads

====================================================================================

graph has 11404 edges initially, 262 edges after simplification

total ucounts = 1084
nonsolo ucounts = 840[2^104, 3^65, 4^57, 5^38, 6^35, 7^26, 8^28, 9^26, 10^25, 11^30, 12^21, 13^25, 14^18, 15^19, 16^16, 17^25, 18^19, 19^16, 20^12, 21^18, 22^15, 23^14, 24^15, 25^19, 26^12, 27^11, 28^8, 29^10, 30^6, 31^3, 32^8, 33^9, 34^4, 35^8, 36^9, 37^5, 38^4, 39^3, 40^4, 41^4, 42, 43^2, 44^4, 45^5, 46^4, 48^2, 49, 50^2, 51, 52, 53, 54^3, 55, 56^2, 63, 69, 72, 76^2, 77, 81, 82, 85, 137, 179, 845, 1501, 2344, 2581, 2681]
surviving nonsolo ucounts = 5[845, 1501, 2344, 2581, 2681]
ids = [749, 718, 1, 157, 151]

====================================================================================

UMI info for barcode TAGTGGTGTCGGATCC-1 contig 1 = AAAAAAAAAA...
umi ACGCAATCGA = 2694 reads: +394 validated
umi ACGCGCTCTC = 2446 reads: -360 +2 -1XX +2 -1XX +1 -2XX +5 -2XX +4 -1XX +1 -3XX +1 -4XX +2 -2XX invalidated
umi GTTTAAAGCA = 846 reads: +394 validated

UMI info for barcode TAGTGGTGTCGGATCC-1 contig 2 = TGGGGACTCT...
umi AAAACACCCG = 2364 reads: +403 validated
umi AAGCAACGGC = 1 reads: -398 +2 -1X +1 -1X invalidated
umi GTCGATATGA = 1467 reads: +403 validated

GOOD CONTIGS

TIG 1[bases=485]
49-379 ==> 0-330 on |501|TRAV35|L-REGION+V-REGION| [len=330] (mis=2)
386-443 ==> 0-57 on |416|TRAJ30|J-REGION| [len=57] (mis=1)
443-485 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 2 umis using 210 reads
cdr3 = CAGQLPGNRDDKIIF at 367, score = 6 + 8
umis assigned: [151, 157, 749]
of which 3 are surviving nonsolos
reads assigned: 5925
start codons at 49, 82, 93, 133, 155, 163, 201, 275, 350, 395
confident = true

TIG 2[bases=468]
13-360 ==> 0-347 on |679|TRBV7-3|L-REGION+V-REGION| [len=347] (mis=4)
367-416 ==> 0-49 on |546|TRBJ2-3|J-REGION| [len=49] (mis=4)
416-468 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 2 umis using 269 reads
cdr3 = CASSSLAHGVDTQYF at 343, score = 8 + 8
umis assigned: [1, 53, 718]
of which 2 are surviving nonsolos
reads assigned: 3742
start codons at 13, 122, 236
confident = true
now this is a cell
paired!

CCTAGTGATGTAGGCATCTACTTCTGTGCTGGGCAGCTACCTGGGAACAGAGATGACAAGATCATCTTTGGAAAAGGGACACGACTTCATATTCTCCCCA <==> GAGCGGGGGGACTCAGCCGTGTATCTCTGTGCCAGCAGTTCACTAGCCCACGGGGTAGATACGCAGTATTTTGGCCCAGGCACCCGGCTGACAGTGCTCG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.957 = TAGTGGTGTCGGCATC-1

using 48 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[7, 41]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.958 = TAGTGGTGTCGTTGTA-1

using 35 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.961 = TAGTGGTGTCTAGTCA-1

using 65025 reads

====================================================================================

graph has 25624 edges initially, 568 edges after simplification

total ucounts = 2422
nonsolo ucounts = 1986[2^171, 3^113, 4^102, 5^86, 6^90, 7^74, 8^85, 9^56, 10^56, 11^60, 12^60, 13^53, 14^58, 15^46, 16^47, 17^47, 18^46, 19^41, 20^38, 21^38, 22^46, 23^43, 24^34, 25^33, 26^40, 27^45, 28^36, 29^20, 30^24, 31^22, 32^25, 33^16, 34^13, 35^18, 36^18, 37^13, 38^10, 39^14, 40^11, 41^10, 42^9, 43^7, 44^6, 45^7, 46^7, 47^7, 48^4, 49^2, 50^5, 51^4, 52^6, 53^6, 54^2, 55^5, 56, 57^2, 58^3, 59, 60^3, 61^5, 62^2, 64^3, 65^2, 67, 68^2, 69^3, 75, 76, 78, 79, 80, 83, 84, 93^2, 108, 136, 501, 781, 793, 809, 1041, 1111, 1114, 2321, 2548, 4697, 7502, 8176]
surviving nonsolo ucounts = 11[781, 793, 809, 1041, 1111, 1114, 2321, 2548, 4697, 7502, 8176]
ids = [1777, 1861, 1915, 509, 1410, 2146, 147, 1163, 2392, 1890, ...]

====================================================================================

UMI info for barcode TAGTGGTGTCTAGTCA-1 contig 1 = CAGAAGCCTC...
umi GATTTTTTCT = 1102 reads: +406 validated
umi TACTCACTCT = 802 reads: +406 validated
umi TGAGCTACTG = 1119 reads: +406 validated

UMI info for barcode TAGTGGTGTCTAGTCA-1 contig 2 = GACTCTGCTC...
umi ATCTAGTGAT = 1056 reads: +397 validated
umi GTTATGGATA = 776 reads: +359 -33X +5 invalidated
umi TAAGTAGCTT = 784 reads: +369 -1 +1 -21 +5 non-validated

GOOD CONTIGS

TIG 1[bases=565]
0-117 ==> 0-117 on |474|TRAV19|5'UTR| [len=117] (mis=0)
117-467 ==> 0-350 on |475|TRAV19|L-REGION+V-REGION| [len=350] (mis=4)
466-523 ==> 6-63 on |431|TRAJ44|J-REGION| [len=63] (mis=0)
523-565 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 3 umis using 127 reads
cdr3 = CALSRAGTASKLTF at 450, score = 7 + 9
umis assigned: [1410, 1915, 2146]
of which 3 are surviving nonsolos
reads assigned: 2974
start codons at 111, 117, 174, 229, 250, 340, 349
confident = true

TIG 2[bases=645]
0-196 ==> 12-208 on |587|TRBV19|5'UTR| [len=208] (mis=0)
196-540 ==> 0-344 on |588|TRBV19|L-REGION+V-REGION| [len=344] (mis=2)
548-593 ==> 8-53 on |541|TRBJ1-6|J-REGION| [len=53] (mis=0)
593-645 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 1 umis using 26 reads
cdr3 = CASSISDPDSPLHF at 523, score = 6 + 9
umis assigned: [509, 1777, 1861]
of which 3 are surviving nonsolos
reads assigned: 2552
start codons at 196, 254, 305, 341, 346, 410
confident = true

REJECT CONTIGS

TIG 1[bases=307]
5-200 ==> 5805-6000 on segment before TRBD1 exon 1 [len=6000] (mis=0)
205-255 ==> 0-50 on |540|TRBJ1-5|J-REGION| [len=50] (mis=5)
255-307 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
umis assigned: [147, 842, 1163, 1275, 1890, 2392]
of which 5 are surviving nonsolos
reads assigned: 23514
start codons at 77, 87, 161, 231
confident = false
did not find CDR3
now this is a cell
paired!

CAAGTCGTGGACTCAGCAGTATACTTCTGTGCTCTGAGTCGCGCCGGCACTGCCAGTAAACTCACCTTTGGGACTGGAACAAGACTTCAGGTCACGCTCG <==> GCCCAAAAGAACCCGACAGCTTTCTATCTCTGTGCCAGTAGTATATCGGACCCAGATTCACCCCTCCACTTTGGGAACGGGACCAGGCTCACTGTGACAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.972 = TAGTGGTGTGCTTCTC-1

using 237 reads

====================================================================================

graph has 298 edges initially, 20 edges after simplification

total ucounts = 19
nonsolo ucounts = 16[2, 3^4, 6, 7, 10, 13, 14, 16, 18, 27^2, 30, 52]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.974 = TAGTGGTGTGTAATGA-1

using 19 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.978 = TAGTGGTGTGTTCGAT-1

using 185 reads

====================================================================================

graph has 199 edges initially, 10 edges after simplification

total ucounts = 16
nonsolo ucounts = 13[2, 3, 5, 7, 8, 12, 13, 17, 19, 21, 22, 24, 29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.980 = TAGTGGTGTGTTTGTG-1

using 32 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.981 = TAGTGGTGTTAAAGAC-1

using 26 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.984 = TAGTGGTGTTATCACG-1

using 46 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[46]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.986 = TAGTGGTGTTCCCTTG-1

using 20 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.987 = TAGTGGTGTTCCTCCA-1

using 115 reads

====================================================================================

graph has 46 edges initially, 4 edges after simplification

total ucounts = 3
nonsolo ucounts = 3[4, 50, 61]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.990 = TAGTGGTGTTGACGTT-1

using 125 reads

====================================================================================

graph has 116 edges initially, 6 edges after simplification

total ucounts = 15
nonsolo ucounts = 11[2^3, 3^3, 15^2, 17, 18, 41]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.993 = TAGTGGTGTTGCCTCT-1

using 853 reads

====================================================================================

graph has 318 edges initially, 6 edges after simplification

total ucounts = 11
nonsolo ucounts = 6[2, 3, 10, 34, 46, 753]
surviving nonsolo ucounts = 1[753]
ids = [10]

====================================================================================

UMI info for barcode TAGTGGTGTTGCCTCT-1 contig 1 = GGGGAACTGT...
umi TGTGTAGTGG = 763 reads: +397 validated

GOOD CONTIGS

TIG 1[bases=469]
30-367 ==> 0-337 on |500|TRAV34|L-REGION+V-REGION| [len=337] (mis=5)
368-427 ==> 7-66 on |432|TRAJ45|J-REGION| [len=66] (mis=0)
427-469 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 1 umis using 49 reads
cdr3 = CGPGRGGGADGLTF at 354, score = 7 + 8
umis assigned: [10]
of which 1 are surviving nonsolos
reads assigned: 746
start codons at 30, 178, 205, 228, 231, 262, 286, 337
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.995 = TAGTGGTGTTGGAGGT-1

using 24 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.1000 = TAGTGGTTCAAACGGG-1

using 48 reads

====================================================================================

graph has 84 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 7[2^3, 3, 4^2, 29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.1003 = TAGTGGTTCAACGGCC-1

using 21161 reads

====================================================================================

graph has 10854 edges initially, 250 edges after simplification

total ucounts = 937
nonsolo ucounts = 711[2^91, 3^48, 4^41, 5^45, 6^23, 7^35, 8^27, 9^24, 10^23, 11^23, 12^18, 13^22, 14^18, 15^15, 16^17, 17^19, 18^12, 19^16, 20^13, 21^12, 22^15, 23^10, 24^4, 25^10, 26^9, 27^5, 28^14, 29^7, 30^8, 31^5, 32^3, 33^4, 34^2, 35^7, 36^3, 37^2, 38^4, 39^2, 40^4, 41^3, 42^3, 44^4, 45^4, 47, 48^3, 49, 50, 51^4, 53^2, 54, 56, 57^2, 60, 61^2, 63, 64, 65, 66, 67, 68, 70, 73, 83, 89, 98, 112, 162, 184, 430, 897, 1788, 6948]
surviving nonsolo ucounts = 6[89, 184, 430, 897, 1788, 6948]
ids = [791, 745, 204, 52, 100, 705]

====================================================================================

UMI info for barcode TAGTGGTTCAACGGCC-1 contig 1 = GTCCTGTCTC...
umi AATTAAGGGT = 902 reads: +397 validated
umi ATACTACAGG = 429 reads: +397 validated
umi TCACTTCTCT = 154 reads: -385 +1 -1X +2 -1XX +2 -4XX +1 invalidated
umi TCTGAGTCAA = 90 reads: +348 -45 +4 non-validated

GOOD CONTIGS

TIG 1[bases=632]
0-183 ==> 25-208 on |589|TRBV19|5'UTR| [len=208] (mis=0)
183-527 ==> 0-344 on |590|TRBV19|L-REGION+V-REGION| [len=344] (mis=1)
531-580 ==> 0-49 on |546|TRBJ2-3|J-REGION| [len=49] (mis=0)
580-632 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 2 umis using 32 reads
cdr3 = CASSIADSTDTQYF at 510, score = 6 + 8
umis assigned: [52, 204, 745, 791]
of which 4 are surviving nonsolos
reads assigned: 1549
start codons at 183, 241, 292, 328, 333, 397
confident = true

REJECT CONTIGS

TIG 1[bases=509]
0-100 ==> 5783-5883 on segment before TRBV5-2 exon 1 [len=6000] (mis=13)
50-393 ==> 0-343 on |631|TRBV5-1|L-REGION+V-REGION| [len=343] (mis=0)
396-408 ==> 0-12 on |535|TRBD1|D-REGION| [len=12] (mis=1)
409-457 ==> 0-48 on |536|TRBJ1-1|J-REGION| [len=48] (mis=1)
457-509 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
umis assigned: [100]
of which 1 are surviving nonsolos
reads assigned: 1757
start codons at 50, 332, 336
confident = false
did not find CDR3
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.1005 = TAGTGGTTCAATAAGG-1

using 197 reads

====================================================================================

graph has 120 edges initially, 10 edges after simplification

total ucounts = 22
nonsolo ucounts = 17[2^4, 3, 4, 5, 9^2, 11, 12, 14, 15, 16, 17, 18, 51]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.1008 = TAGTGGTTCACCAGGC-1

using 39 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[39]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 78.1009 = TAGTGGTTCACTTCAT-1

using 151 reads

====================================================================================

graph has 198 edges initially, 6 edges after simplification

total ucounts = 19
nonsolo ucounts = 17[2^5, 3, 4^3, 5, 6, 7, 10, 15, 19, 28, 34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!
sorting bam, mem = 0.16
running samtools sort -l 8G -o /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_T_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk78-ucc6a7a72e6/files/contig_bam_sorted.bam /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_T_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk78-ucc6a7a72e6/files/contig_bam.bam

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76.512 seconds used processing barcodes, peak mem = 0.32
