[stdout]

starting vdj_asm_asm, mem = 0.01
available mem = 827.89 GB
/scratch/etanis/software/cellranger-6.1.2/lib/bin/cr_vdj martian assembly main /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_T_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk72-ucc6a7a72e6 /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_T_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk72-ucc6a7a72e6/files /scratch/etanis/ST-118/multi/dimitri-multi/journal/SC_MULTI_CS.SC_MULTI_CORE.MULTI_GEM_WELL_PROCESSOR.VDJ_T_GEM_WELL_PROCESSOR.SC_VDJ_CONTIG_ASSEMBLER.ASSEMBLE_VDJ.fork0.chnk72.ucc6a7a72e6
n50_n50_rpu = 2159

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.0 = GTGCATATCAGGTTCA-1

using 242 reads

====================================================================================

graph has 249 edges initially, 16 edges after simplification

total ucounts = 12
nonsolo ucounts = 9[3^2, 4, 8, 11, 22, 47, 70, 71]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.3 = GTGCATATCATAGCAC-1

using 70 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[70]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.7 = GTGCATATCATTGCGA-1

using 66 reads

====================================================================================

graph has 84 edges initially, 2 edges after simplification

total ucounts = 13
nonsolo ucounts = 8[2^2, 3, 6, 7, 11, 15^2]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.8 = GTGCATATCCAAACTG-1

using 94 reads

====================================================================================

graph has 74 edges initially, 4 edges after simplification

total ucounts = 4
nonsolo ucounts = 4[8, 11, 29, 46]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.10 = GTGCATATCCAGAGGA-1

using 25076 reads

====================================================================================

graph has 13880 edges initially, 390 edges after simplification

total ucounts = 1237
nonsolo ucounts = 951[2^115, 3^73, 4^73, 5^60, 6^44, 7^45, 8^28, 9^33, 10^32, 11^24, 12^27, 13^29, 14^33, 15^23, 16^25, 17^34, 18^24, 19^19, 20^21, 21^14, 22^17, 23^12, 24^10, 25^9, 26^13, 27^9, 28^9, 29^8, 30^12, 31^5, 32^9, 33^6, 34^5, 35^4, 36^3, 37^2, 38^4, 39^3, 42^3, 43^3, 46, 47, 49^2, 50^2, 52^2, 53, 54^3, 60, 61, 68, 70, 73, 88, 89, 91, 95^2, 99, 107, 137, 465, 1455, 2942, 7270]
surviving nonsolo ucounts = 4[465, 1455, 2942, 7270]
ids = [588, 1195, 782, 244]

====================================================================================

REJECT CONTIGS

TIG 1[bases=309]
13-84 ==> 0-71 on segment before TRBJ2-5 exon 1 [len=71] (mis=0)
84-132 ==> 0-48 on |548|TRBJ2-5|J-REGION| [len=48] (mis=0)
132-204 ==> 0-72 on segment before TRBJ2-6 exon 1 [len=72] (mis=0)
257-309 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
umis assigned: [244, 588, 782, 1049]
of which 3 are surviving nonsolos
reads assigned: 10451
start codons at 
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.21 = GTGCATATCCTTGGTC-1

using 52700 reads

====================================================================================

graph has 13783 edges initially, 202 edges after simplification

total ucounts = 584
nonsolo ucounts = 435[2^59, 3^40, 4^30, 5^35, 6^23, 7^20, 8^24, 9^21, 10^11, 11^12, 12^9, 13^12, 14^11, 15^4, 16^13, 17^9, 18^11, 19^7, 20^10, 21^12, 22^2, 23^3, 24^7, 25^2, 26, 27^2, 28^2, 29, 30^2, 31^3, 32, 33, 34, 35^3, 36, 39, 40, 41, 45, 48, 49, 50, 52, 53, 58, 59, 62, 184, 359, 828, 837, 933, 1000, 1025, 1040, 1061, 1909, 1924, 3029, 3458, 3543, 4720, 5708, 8106, 8346]
surviving nonsolo ucounts = 16[828, 837, 933, 1000, 1025, 1040, 1061, 1909, 1924, 3029, 3458, 3543, 4720, 5708, 8106, 8346]
ids = [152, 9, 198, 456, 246, 299, 418, 107, 509, 39, ...]

====================================================================================

UMI info for barcode GTGCATATCCTTGGTC-1 contig 1 = AGATCAGAAG...
umi AACAATTCCT = 843 reads: +409 validated
umi AATCTCTCGT = 3013 reads: +361 -1XX +2 -1XX +4 -2XX +1 -2XX +1 -7XX +1 -1X +1 -5X +2 -5XX +2 -3XX +2 -2XX +1 -2XX invalidated
umi ATAAGGCCGT = 1909 reads: +409 validated
umi GTCCGGACCA = 3463 reads: +361 -1XX +2 -1XX +4 -2XX +1 -2XX +1 -7XX +1 -1XX +1 -5XX +2 -5XX +2 -3XX +2 -2XX +1 -2XX invalidated
umi TGTGATCACT = 1928 reads: +409 validated

UMI info for barcode GTGCATATCCTTGGTC-1 contig 2 = GTCCTGTCTC...
umi ATTGCATTTC = 831 reads: +400 validated
umi CCGAATTATC = 904 reads: +387 -1X +12 invalidated
umi CTACGCTCCT = 1031 reads: +381 -12 +7 non-validated
umi GACTTGTGTC = 1040 reads: +400 validated
umi TATCCCATTA = 1059 reads: +400 validated
umi TCGTAACACG = 1003 reads: +384 -4 +3 -1 +8 non-validated

GOOD CONTIGS

TIG 1[bases=497]
0-46 ==> 0-46 on |506|TRAV38-2/DV8|5'UTR| [len=46] (mis=0)
46-395 ==> 0-349 on |507|TRAV38-2/DV8|L-REGION+V-REGION| [len=349] (mis=0)
399-455 ==> 0-56 on |436|TRAJ49|J-REGION| [len=56] (mis=3)
455-497 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 3 umis using 244 reads
cdr3 = CAYRSASITGNQFYF at 379, score = 7 + 7
umis assigned: [9, 39, 107, 360, 509]
of which 5 are surviving nonsolos
reads assigned: 11000
start codons at 46, 51, 103, 136, 179, 238, 278, 362, 370
confident = true

TIG 2[bases=635]
0-183 ==> 25-208 on |587|TRBV19|5'UTR| [len=208] (mis=0)
183-527 ==> 0-344 on |588|TRBV19|L-REGION+V-REGION| [len=344] (mis=1)
546-583 ==> 11-48 on |536|TRBJ1-1|J-REGION| [len=48] (mis=0)
583-635 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 3 umis using 86 reads
cdr3 = CASSIGDSTPFGAFF at 510, score = 6 + 8
umis assigned: [152, 198, 246, 299, 418, 456]
of which 6 are surviving nonsolos
reads assigned: 5778
start codons at 183, 241, 292, 328, 333, 397
confident = true

REJECT CONTIGS

TIG 1[bases=387]
5-281 ==> 2301-2577 on segment before TRBJ2-1 exon 1 [len=3226] (mis=0)
285-335 ==> 0-50 on |543|TRBJ2-1|J-REGION| [len=50] (mis=0)
335-387 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
umis assigned: [291, 400, 414, 520]
of which 4 are surviving nonsolos
reads assigned: 22970
start codons at 38, 55, 59, 254, 293
confident = false
did not find CDR3

TIG 2[bases=436]
15-340 ==> 0-325 on |481|TRAV21|L-REGION+V-REGION| [len=336] (mis=0)
346-394 ==> 14-62 on |423|TRAJ37|J-REGION| [len=62] (mis=0)
394-436 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
umis assigned: [569]
of which 1 are surviving nonsolos
reads assigned: 3509
start codons at 15, 59, 259
confident = false
full length transcript of length 436
did not find CDR3
now this is a cell
paired!

CTGGGGGATGCCGCGATGTATTTCTGTGCTTATAGGAGCGCGTCGATAACGGGTAACCAGTTCTATTTTGGGACAGGGACAAGTTTGACGGTCATTCCAA <==> CAAAAGAACCCGACAGCTTTCTATCTCTGTGCCAGTAGTATAGGGGACAGCACCCCCTTTGGGGCTTTCTTTGGACAAGGCACCAGACTCACAGTTGTAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.24 = GTGCATATCGAATGGG-1

using 59 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[58]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.25 = GTGCATATCGAGAGCA-1

using 80 reads

====================================================================================

graph has 72 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[80]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.28 = GTGCATATCGTACCGG-1

using 18 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.29 = GTGCATATCGTCCGTT-1

using 69 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 3[2, 12, 51]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.32 = GTGCATATCTACGAGT-1

using 77 reads

====================================================================================

graph has 72 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 5[8, 12, 17, 18, 19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.33 = GTGCATATCTCCAGGG-1

using 19 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[8, 11]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.35 = GTGCATATCTGCAGTA-1

using 12888 reads

====================================================================================

graph has 7480 edges initially, 212 edges after simplification

total ucounts = 671
nonsolo ucounts = 492[2^101, 3^33, 4^32, 5^24, 6^28, 7^26, 8^11, 9^23, 10^12, 11^23, 12^8, 13^14, 14^9, 15^10, 16^7, 17^10, 18^10, 19^17, 20^9, 21^5, 22^9, 23^11, 24^5, 25^7, 26^8, 27^2, 28, 29, 31^3, 32^4, 34^3, 35, 36^3, 37, 40, 42, 43^3, 44^2, 45, 47, 52, 58, 59^2, 60, 63^2, 82, 84, 96, 107, 6776]
surviving nonsolo ucounts = 1[6776]
ids = [548]

====================================================================================

REJECT CONTIGS

TIG 1[bases=301]
9-199 ==> 3036-3226 on segment before TRBJ2-1 exon 1 [len=3226] (mis=0)
199-249 ==> 0-50 on |543|TRBJ2-1|J-REGION| [len=50] (mis=0)
249-301 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
umis assigned: [548]
of which 1 are surviving nonsolos
reads assigned: 6664
start codons at 58, 207
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.40 = GTGCGGTAGAAACCAT-1

using 55 reads

====================================================================================

graph has 48 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 5[2^2, 5, 7, 34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.42 = GTGCGGTAGAACTGTA-1

using 93 reads

====================================================================================

graph has 110 edges initially, 2 edges after simplification

total ucounts = 14
nonsolo ucounts = 10[3, 4, 5, 6^2, 8, 10, 12, 16, 19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.44 = GTGCGGTAGACAATAC-1

using 112 reads

====================================================================================

graph has 148 edges initially, 4 edges after simplification

total ucounts = 24
nonsolo ucounts = 14[2, 3^2, 4^4, 5, 6, 7, 11, 12, 16, 21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.47 = GTGCGGTAGAGCCTAG-1

using 171 reads

====================================================================================

graph has 192 edges initially, 8 edges after simplification

total ucounts = 14
nonsolo ucounts = 11[2^2, 3, 8, 9^2, 16, 17, 21, 23, 58]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.51 = GTGCGGTAGATAGCAT-1

using 403 reads

====================================================================================

graph has 378 edges initially, 14 edges after simplification

total ucounts = 30
nonsolo ucounts = 27[2, 3^3, 4^2, 5, 6^2, 8, 9, 11^3, 12, 14^3, 19, 20, 22, 23^2, 25, 30, 49^2]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.53 = GTGCGGTAGATGTGTA-1

using 94 reads

====================================================================================

graph has 122 edges initially, 2 edges after simplification

total ucounts = 23
nonsolo ucounts = 16[2^4, 3^2, 5^2, 6^3, 7, 8, 9, 10, 11]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.54 = GTGCGGTAGCAACGGT-1

using 303 reads

====================================================================================

graph has 287 edges initially, 12 edges after simplification

total ucounts = 24
nonsolo ucounts = 18[2^2, 3^2, 5^2, 8, 9, 14^3, 15, 18^2, 21^2, 56, 69]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.55 = GTGCGGTAGCAGCGTA-1

using 115 reads

====================================================================================

graph has 136 edges initially, 2 edges after simplification

total ucounts = 14
nonsolo ucounts = 9[3, 4, 5^2, 7, 20, 21, 22, 23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.63 = GTGCGGTAGCTAAACA-1

using 77 reads

====================================================================================

graph has 86 edges initially, 2 edges after simplification

total ucounts = 15
nonsolo ucounts = 11[2^2, 4^4, 7, 9, 10, 13, 14]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.66 = GTGCGGTAGCTCCCAG-1

using 47 reads

====================================================================================

graph has 62 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 5[2^2, 8, 10, 24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.68 = GTGCGGTAGCTTATCG-1

using 148 reads

====================================================================================

graph has 188 edges initially, 4 edges after simplification

total ucounts = 17
nonsolo ucounts = 15[2^2, 4, 5, 6, 9, 10, 11, 12^2, 13^3, 15, 19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.70 = GTGCGGTAGGCCGAAT-1

using 118 reads

====================================================================================

graph has 148 edges initially, 2 edges after simplification

total ucounts = 14
nonsolo ucounts = 8[2^3, 6, 13, 26^2, 35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.73 = GTGCGGTAGGCTCAGA-1

using 274 reads

====================================================================================

graph has 156 edges initially, 8 edges after simplification

total ucounts = 20
nonsolo ucounts = 17[2^4, 4^3, 6, 8, 9, 11, 14, 19, 21, 27, 35, 101]
surviving nonsolo ucounts = 1[101]
ids = [10]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.74 = GTGCGGTAGGTGCTTT-1

using 182 reads

====================================================================================

graph has 262 edges initially, 4 edges after simplification

total ucounts = 19
nonsolo ucounts = 13[2^2, 3, 4, 5, 7, 10, 14, 16, 17, 22, 31, 43]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.75 = GTGCGGTAGGTGTGGT-1

using 252 reads

====================================================================================

graph has 350 edges initially, 8 edges after simplification

total ucounts = 33
nonsolo ucounts = 22[2^5, 4, 5, 6^2, 8^3, 12^2, 14, 15, 17, 18^2, 20, 29, 31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.76 = GTGCGGTAGTAGTGCG-1

using 29 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.77 = GTGCGGTAGTATCTCG-1

using 153 reads

====================================================================================

graph has 148 edges initially, 8 edges after simplification

total ucounts = 18
nonsolo ucounts = 15[2, 3^2, 5^4, 8^3, 16^3, 17, 33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.79 = GTGCGGTAGTCCGTAT-1

using 243 reads

====================================================================================

graph has 233 edges initially, 4 edges after simplification

total ucounts = 29
nonsolo ucounts = 24[2^5, 3^2, 4, 5, 6^3, 8, 9, 12^2, 13, 15, 16, 17, 18, 21, 24, 30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.80 = GTGCGGTAGTGATCGG-1

using 81 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[81]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.83 = GTGCGGTAGTTACCCA-1

using 55 reads

====================================================================================

graph has 60 edges initially, 2 edges after simplification

total ucounts = 14
nonsolo ucounts = 10[2^3, 3^2, 4, 5, 6, 9, 15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.85 = GTGCGGTAGTTGTAGA-1

using 25 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.87 = GTGCGGTCAAAGCGGT-1

using 2643 reads

====================================================================================

graph has 1426 edges initially, 38 edges after simplification

total ucounts = 175
nonsolo ucounts = 127[2^28, 3^8, 4^6, 5^7, 6^5, 7^6, 8^5, 9^5, 10, 11^3, 12, 13^2, 15, 16, 17, 19^2, 20, 22, 23, 24^2, 25^2, 28^3, 30^3, 31, 33, 34^3, 36^3, 38^3, 39, 41^2, 42, 51^2, 52^2, 53, 55, 61, 64, 72, 75, 79, 91^2, 92, 98, 102, 105]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.88 = GTGCGGTCAAATTGCC-1

using 121 reads

====================================================================================

graph has 108 edges initially, 4 edges after simplification

total ucounts = 15
nonsolo ucounts = 10[2, 4, 6, 7^2, 13^2, 14, 16, 34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.90 = GTGCGGTCAACGATCT-1

using 120 reads

====================================================================================

graph has 148 edges initially, 2 edges after simplification

total ucounts = 12
nonsolo ucounts = 11[2, 3^2, 5, 6, 7, 8, 10, 12, 14, 49]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.91 = GTGCGGTCAACTGCTA-1

using 66 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[66]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.92 = GTGCGGTCAAGCCATT-1

using 189 reads

====================================================================================

graph has 241 edges initially, 4 edges after simplification

total ucounts = 19
nonsolo ucounts = 16[3^6, 5^2, 6, 7, 11, 12^2, 30^2, 50]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.94 = GTGCGGTCAATGCCAT-1

using 210 reads

====================================================================================

graph has 225 edges initially, 10 edges after simplification

total ucounts = 21
nonsolo ucounts = 15[2^2, 3^2, 4^2, 8, 10, 11, 13, 17, 19, 30, 35, 43]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.95 = GTGCGGTCAATGTAAG-1

using 1253 reads

====================================================================================

graph has 591 edges initially, 10 edges after simplification

total ucounts = 20
nonsolo ucounts = 12[2^2, 8, 10, 11^3, 17^2, 24, 35, 1097]
surviving nonsolo ucounts = 1[1097]
ids = [5]

====================================================================================

UMI info for barcode GTGCGGTCAATGTAAG-1 contig 1 = AGACACAGTG...
umi CGTCAAGAGG = 1112 reads: +400 validated

GOOD CONTIGS

TIG 1[bases=481]
0-29 ==> 280-309 on |630|TRBV5-1|5'UTR| [len=309] (mis=0)
29-372 ==> 0-343 on |631|TRBV5-1|L-REGION+V-REGION| [len=343] (mis=0)
373-385 ==> 0-12 on |535|TRBD1|D-REGION| [len=12] (mis=3)
382-429 ==> 0-47 on |550|TRBJ2-7|J-REGION| [len=47] (mis=2)
429-481 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 1 umis using 83 reads
cdr3 = CASSLDRQGPYEQYF at 356, score = 9 + 8
umis assigned: [5]
of which 1 are surviving nonsolos
reads assigned: 1090
start codons at 29, 311, 315
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.96 = GTGCGGTCACAACGTT-1

using 82 reads

====================================================================================

graph has 52 edges initially, 4 edges after simplification

total ucounts = 12
nonsolo ucounts = 9[2, 3, 4^2, 5^2, 6, 16, 34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.97 = GTGCGGTCACAAGTAA-1

using 139 reads

====================================================================================

graph has 208 edges initially, 6 edges after simplification

total ucounts = 18
nonsolo ucounts = 11[2, 3, 4, 9, 10^2, 11^2, 17, 20, 35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.99 = GTGCGGTCACCACCAG-1

using 31 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.100 = GTGCGGTCACCAGATT-1

using 92 reads

====================================================================================

graph has 144 edges initially, 2 edges after simplification

total ucounts = 17
nonsolo ucounts = 12[2^2, 3^2, 4^2, 5, 6, 7, 8, 12, 31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.102 = GTGCGGTCACCGGAAA-1

using 77 reads

====================================================================================

graph has 70 edges initially, 4 edges after simplification

total ucounts = 9
nonsolo ucounts = 6[4, 8, 11, 12, 13, 26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.104 = GTGCGGTCACGACTCG-1

using 188 reads

====================================================================================

graph has 138 edges initially, 10 edges after simplification

total ucounts = 15
nonsolo ucounts = 11[2^2, 3, 4, 8, 9^2, 15, 17, 22, 93]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.105 = GTGCGGTCACGGTGTC-1

using 91 reads

====================================================================================

graph has 56 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[91]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.106 = GTGCGGTCACTTCGAA-1

using 112 reads

====================================================================================

graph has 92 edges initially, 2 edges after simplification

total ucounts = 15
nonsolo ucounts = 10[2^2, 7, 9, 10^2, 11, 13, 21, 22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.107 = GTGCGGTCAGAAGCAC-1

using 18 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.110 = GTGCGGTCAGATAATG-1

using 71 reads

====================================================================================

graph has 70 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 8[2^2, 4^2, 7, 8, 14, 27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.116 = GTGCGGTCAGGAACGT-1

using 138 reads

====================================================================================

graph has 118 edges initially, 4 edges after simplification

total ucounts = 22
nonsolo ucounts = 14[2^3, 4^2, 6, 7, 8, 10, 13, 14^2, 16, 28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.120 = GTGCGGTCAGGGCATA-1

using 78 reads

====================================================================================

graph has 72 edges initially, 4 edges after simplification

total ucounts = 13
nonsolo ucounts = 11[2^3, 3, 5^2, 7, 10, 13^2, 14]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.122 = GTGCGGTCATATGAGA-1

using 200 reads

====================================================================================

graph has 256 edges initially, 12 edges after simplification

total ucounts = 31
nonsolo ucounts = 20[2^4, 3^2, 4^2, 5, 6, 8, 9^2, 11, 13, 14, 15, 16, 25, 36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.123 = GTGCGGTCATCACGTA-1

using 212 reads

====================================================================================

graph has 218 edges initially, 12 edges after simplification

total ucounts = 21
nonsolo ucounts = 17[2^4, 3^2, 4^2, 6, 10, 12^2, 19, 21, 22, 37, 47]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.124 = GTGCGGTCATCCTTGC-1

using 33 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.126 = GTGCGGTCATCTCCCA-1

using 97 reads

====================================================================================

graph has 112 edges initially, 6 edges after simplification

total ucounts = 12
nonsolo ucounts = 8[2, 4, 6, 7, 9, 15, 20, 30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.127 = GTGCGGTCATCTCGCT-1

using 20141 reads

====================================================================================

graph has 13202 edges initially, 316 edges after simplification

total ucounts = 1722
nonsolo ucounts = 1325[2^181, 3^129, 4^103, 5^73, 6^70, 7^74, 8^63, 9^56, 10^47, 11^32, 12^28, 13^41, 14^30, 15^18, 16^21, 17^24, 18^19, 19^17, 20^17, 21^11, 22^16, 23^19, 24^15, 25^14, 26^11, 27^11, 28^8, 29^19, 30^9, 31^7, 32^6, 33^3, 34^6, 35^5, 36^7, 37^5, 38^2, 39^6, 40^3, 41^3, 42^7, 43^4, 44, 45^3, 46^2, 48^3, 49^2, 50^4, 51^4, 53, 54^2, 55^2, 56^3, 57^3, 58^2, 60^2, 61^2, 62, 64, 65, 67^5, 68^3, 69, 70, 71, 72, 73^3, 74, 75, 77, 79^4, 82^2, 83, 84^2, 85^2, 86^2, 89, 93, 94^2, 95^2, 97, 98, 101, 103, 112, 121, 138, 145, 551]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.129 = GTGCGGTCATGCATGT-1

using 34831 reads

====================================================================================

graph has 13248 edges initially, 248 edges after simplification

total ucounts = 986
nonsolo ucounts = 751[2^81, 3^56, 4^57, 5^57, 6^52, 7^34, 8^37, 9^41, 10^28, 11^23, 12^14, 13^24, 14^13, 15^18, 16^8, 17^10, 18^10, 19^12, 20^11, 21^11, 22^8, 23^12, 24^2, 25^8, 26^2, 27^5, 28^2, 29^3, 30^3, 31^2, 32^2, 33^2, 34^5, 35^3, 36^5, 37^3, 38, 39^3, 40^2, 41^2, 43^4, 44^2, 45^4, 46, 47^3, 48^2, 50, 51^3, 52^3, 53, 54^2, 55^3, 56, 57^2, 59^2, 62, 64, 65^2, 67, 69^2, 72^4, 74, 75, 76, 77, 78, 79, 80, 81, 84, 85, 86, 88^3, 89, 90^2, 94, 99, 102, 104, 123, 256, 265, 281, 288, 966, 1446, 1724, 1819, 2058, 2662, 3560, 8019]
surviving nonsolo ucounts = 11[123, 256, 288, 966, 1446, 1724, 1819, 2058, 2662, 3560, 8019]
ids = [67, 325, 902, 910, 736, 566, 610, 573, 417, 942, ...]

====================================================================================

UMI info for barcode GTGCGGTCATGCATGT-1 contig 1 = AGATCAGAAG...
umi GAGTCAGTCA = 7088 reads: -369X +1 -2XX +7 -1XX +3 -1XX +2 -5XX +1 -1XX +1 -2XX +2 -5XX +3 invalidated
umi GCGTCCCTGT = 1721 reads: +406 validated
umi GCTTCGCCTC = 2082 reads: +406 validated
umi TATGGTTATC = 1456 reads: +406 validated
umi TTATTCGGGA = 975 reads: +406 validated
umi TTGCCCCTTG = 3376 reads: -368X +11 -1XX +6 -1XX +1 -2XX +2 -4XX +3 -2XX +1 -1XX +3 invalidated

UMI info for barcode GTGCGGTCATGCATGT-1 contig 2 = GAGTGTCCTG...
umi ACATCATTAC = 91 reads: -375X +3 -2X +1 -5XX +2 -1XX +2 invalidated
umi AGGAGTTCGA = 1 reads: -387 +2 -2X invalidated
umi CCCACGGCAT = 257 reads: +391 validated
umi CGTCCACGTC = 2367 reads: -370 +5 -1XX +2 -1XX +1 -2XX +1 -1XX +2 -2XX +1 -1XX +1 invalidated
umi GGTATGGCTG = 1821 reads: +391 validated
umi TTAGCACAGG = 239 reads: -373 +2 -1X +2 -1X +1 -1XX +2 -1XX +2 -2XX +1 -1XX +1 invalidated

GOOD CONTIGS

TIG 1[bases=494]
0-46 ==> 0-46 on |506|TRAV38-2/DV8|5'UTR| [len=46] (mis=0)
46-395 ==> 0-349 on |507|TRAV38-2/DV8|L-REGION+V-REGION| [len=349] (mis=2)
391-452 ==> 0-61 on |427|TRAJ40|J-REGION| [len=61] (mis=4)
452-494 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 4 umis using 372 reads
cdr3 = CAYRPDSGTYKYIF at 379, score = 7 + 8
umis assigned: [524, 566, 573, 736, 910, 942]
of which 6 are surviving nonsolos
reads assigned: 16531
start codons at 46, 51, 103, 136, 179, 238, 278, 362, 370
confident = true

TIG 2[bases=612]
169-512 ==> 0-343 on |643|TRBV5-6|L-REGION+V-REGION| [len=343] (mis=1)
520-560 ==> 8-48 on |537|TRBJ1-2|J-REGION| [len=48] (mis=0)
560-612 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 2 umis using 129 reads
cdr3 = CASSLRVSGYTF at 496, score = 8 + 9
umis assigned: [67, 150, 325, 417, 610, 902]
of which 5 are surviving nonsolos
reads assigned: 4740
start codons at 35, 142, 169, 291, 311, 374, 455
confident = true
now this is a cell
paired!

CAGCTGGGGGATGCCGCGATGTATTTCTGTGCTTATAGGCCCGACTCAGGAACCTACAAATACATCTTTGGAACAGGCACCAGGCTGAAGGTTTTAGCAA <==> AACGCCTTGTTGCTGGGGGACTCGGCCCTCTATCTCTGTGCCAGCAGCTTGCGAGTTTCAGGCTACACCTTCGGTTCGGGGACCAGGTTAACCGTTGTAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.132 = GTGCGGTCATGTCGAT-1

using 87 reads

====================================================================================

graph has 130 edges initially, 2 edges after simplification

total ucounts = 12
nonsolo ucounts = 7[4, 5, 7^2, 17, 21^2]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.133 = GTGCGGTCATGTCTCC-1

using 287 reads

====================================================================================

graph has 242 edges initially, 16 edges after simplification

total ucounts = 22
nonsolo ucounts = 18[2^2, 3, 4^4, 5, 8, 14, 19, 20, 23^2, 26, 39, 41, 42]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.134 = GTGCGGTCATGTTGAC-1

using 41 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[41]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.135 = GTGCGGTCATTACGAC-1

using 33 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.138 = GTGCGGTCATTTCACT-1

using 53 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[53]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.143 = GTGCGGTGTAAGTGTA-1

using 170 reads

====================================================================================

graph has 150 edges initially, 8 edges after simplification

total ucounts = 14
nonsolo ucounts = 12[2, 3^3, 6, 7, 8, 16, 18, 19, 22, 61]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.146 = GTGCGGTGTACATCCA-1

using 78 reads

====================================================================================

graph has 66 edges initially, 4 edges after simplification

total ucounts = 15
nonsolo ucounts = 12[2^2, 3^2, 4, 5, 6^2, 8, 10, 12, 14]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.148 = GTGCGGTGTAGAAGGA-1

using 136 reads

====================================================================================

graph has 173 edges initially, 4 edges after simplification

total ucounts = 18
nonsolo ucounts = 16[2^2, 3^5, 4^2, 7, 8, 12, 15, 19, 23^2]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.152 = GTGCGGTGTCAAAGAT-1

using 100 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[100]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.155 = GTGCGGTGTCATATCG-1

using 85 reads

====================================================================================

graph has 102 edges initially, 4 edges after simplification

total ucounts = 12
nonsolo ucounts = 9[2^3, 5, 10, 12, 13, 16, 20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.159 = GTGCGGTGTCCCTACT-1

using 46 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[46]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.160 = GTGCGGTGTCCGTCAG-1

using 100 reads

====================================================================================

graph has 48 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[99]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.164 = GTGCGGTGTCGGCTCA-1

using 14 reads

====================================================================================

graph has 2 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[14]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.165 = GTGCGGTGTCTAGCCG-1

using 136 reads

====================================================================================

graph has 76 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[9, 126]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.166 = GTGCGGTGTCTAGCGC-1

using 31 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.167 = GTGCGGTGTCTAGTCA-1

using 174 reads

====================================================================================

graph has 284 edges initially, 10 edges after simplification

total ucounts = 40
nonsolo ucounts = 21[2^2, 3^5, 4, 5^2, 6, 7^2, 8, 11^2, 12, 13, 14, 16, 17]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.168 = GTGCGGTGTCTCACCT-1

using 157 reads

====================================================================================

graph has 172 edges initially, 4 edges after simplification

total ucounts = 14
nonsolo ucounts = 13[2^2, 4^2, 5, 9^2, 11, 13, 17, 18, 28, 34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.172 = GTGCGGTGTCTGGTCG-1

using 101 reads

====================================================================================

graph has 108 edges initially, 6 edges after simplification

total ucounts = 17
nonsolo ucounts = 12[2^2, 3^3, 5, 6, 8, 10, 16, 17, 21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.177 = GTGCGGTGTGCAACGA-1

using 196 reads

====================================================================================

graph has 254 edges initially, 6 edges after simplification

total ucounts = 30
nonsolo ucounts = 20[2^3, 4^2, 6, 7^3, 9^2, 11^2, 12^2, 13^2, 14, 20, 21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.183 = GTGCGGTGTTAAAGTG-1

using 109 reads

====================================================================================

graph has 130 edges initially, 4 edges after simplification

total ucounts = 14
nonsolo ucounts = 9[2^2, 5, 9, 10, 13^2, 24, 25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.186 = GTGCGGTGTTCAGGCC-1

using 55 reads

====================================================================================

graph has 74 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 6[2^2, 7, 9, 11, 20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.187 = GTGCGGTGTTGCTCCT-1

using 89 reads

====================================================================================

graph has 116 edges initially, 2 edges after simplification

total ucounts = 16
nonsolo ucounts = 10[2^4, 5^3, 7, 13, 40]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.190 = GTGCGGTGTTTGACTG-1

using 31951 reads

====================================================================================

graph has 11596 edges initially, 200 edges after simplification

total ucounts = 1104
nonsolo ucounts = 852[2^95, 3^87, 4^62, 5^65, 6^44, 7^47, 8^52, 9^38, 10^29, 11^35, 12^32, 13^23, 14^22, 15^19, 16^25, 17^19, 18^14, 19^16, 20^14, 21^19, 22^12, 23^13, 24^8, 25^5, 26^2, 27^7, 28^5, 29, 30^3, 31^3, 32, 33, 34^4, 35^4, 36, 37^5, 39, 42^2, 43, 44, 45, 55, 56, 61^2, 191, 198, 730, 1434, 1751, 1847, 2099, 2246, 4197, 8198]
surviving nonsolo ucounts = 8[730, 1434, 1751, 1847, 2099, 2246, 4197, 8198]
ids = [744, 543, 994, 384, 198, 970, 408, 30]

====================================================================================

UMI info for barcode GTGCGGTGTTTGACTG-1 contig 1 = CTTTTCTCAG...
umi AGTCATCGTA = 2120 reads: +400 validated
umi CCCGTCACCA = 1855 reads: +400 validated
umi CTCCTTCGTC = 1434 reads: +400 validated
umi GGTTCTACTC = 733 reads: +400 validated
umi TGCCCTAGGT = 2245 reads: +400 validated
umi TGTACACGGT = 1757 reads: +400 validated

GOOD CONTIGS

TIG 1[bases=483]
0-31 ==> 36-67 on |614|TRBV29-1|5'UTR| [len=67] (mis=0)
31-366 ==> 0-335 on |615|TRBV29-1|L-REGION+V-REGION| [len=335] (mis=2)
390-431 ==> 9-50 on |543|TRBJ2-1|J-REGION| [len=50] (mis=0)
431-483 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 6 umis using 640 reads
cdr3 = CSVEFLAGGIAMGEQFF at 352, score = 7 + 8
umis assigned: [198, 384, 543, 744, 970, 994]
of which 6 are surviving nonsolos
reads assigned: 9969
start codons at 31, 166, 169, 245, 322, 385
confident = true

REJECT CONTIGS

TIG 1[bases=308]
5-200 ==> 5805-6000 on segment before TRBD1 exon 1 [len=6000] (mis=0)
206-256 ==> 0-50 on |540|TRBJ1-5|J-REGION| [len=50] (mis=4)
256-308 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
umis assigned: [30]
of which 1 are surviving nonsolos
reads assigned: 8112
start codons at 77, 87, 161, 232
confident = false
did not find CDR3

TIG 2[bases=384]
27-131 ==> 0-104 on segment before TRBJ2-3 exon 1 [len=104] (mis=0)
131-180 ==> 0-49 on |546|TRBJ2-3|J-REGION| [len=49] (mis=0)
180-282 ==> 0-102 on segment before TRBJ2-4 exon 1 [len=102] (mis=0)
332-384 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
umis assigned: [408]
of which 1 are surviving nonsolos
reads assigned: 4142
start codons at 41
confident = false
did not find CDR3
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.191 = GTGCGGTTCAAACGGG-1

using 48 reads

====================================================================================

graph has 38 edges initially, 4 edges after simplification

total ucounts = 10
nonsolo ucounts = 5[3, 5, 10^2, 15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.194 = GTGCGGTTCACATACG-1

using 294 reads

====================================================================================

graph has 270 edges initially, 16 edges after simplification

total ucounts = 39
nonsolo ucounts = 26[2^3, 3, 4^4, 5, 6, 7, 8, 9, 10^2, 11, 12^2, 13^2, 14, 15, 20, 28, 31, 32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.199 = GTGCGGTTCAGGCCCA-1

using 67 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[67]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.203 = GTGCGGTTCCAAGCCG-1

using 21 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.204 = GTGCGGTTCCACGAAT-1

using 263 reads

====================================================================================

graph has 246 edges initially, 4 edges after simplification

total ucounts = 46
nonsolo ucounts = 31[2^10, 3, 4, 5^2, 6^2, 7, 8^2, 10^3, 11, 12, 13^2, 14, 15, 17^2, 34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.205 = GTGCGGTTCCACTCCA-1

using 243 reads

====================================================================================

graph has 174 edges initially, 8 edges after simplification

total ucounts = 32
nonsolo ucounts = 20[2, 3^3, 5^4, 7, 10, 11^2, 12, 14, 15, 17^2, 19, 32, 35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.208 = GTGCGGTTCCAGTATG-1

using 140 reads

====================================================================================

graph has 114 edges initially, 4 edges after simplification

total ucounts = 25
nonsolo ucounts = 16[2^4, 3^2, 4, 7^2, 8^3, 14, 16, 18, 27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.209 = GTGCGGTTCCCTCAGT-1

using 90 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[90]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.211 = GTGCGGTTCCGATATG-1

using 54 reads

====================================================================================

graph has 112 edges initially, 2 edges after simplification

total ucounts = 14
nonsolo ucounts = 5[4^2, 11, 12, 14]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.212 = GTGCGGTTCCGCATCT-1

using 194 reads

====================================================================================

graph has 200 edges initially, 6 edges after simplification

total ucounts = 22
nonsolo ucounts = 18[2^7, 4, 7, 10, 16^2, 18^2, 19, 20, 24^2]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.214 = GTGCGGTTCCGTACAA-1

using 134 reads

====================================================================================

graph has 164 edges initially, 6 edges after simplification

total ucounts = 18
nonsolo ucounts = 12[2^3, 4, 5^2, 6^2, 14, 15, 29, 38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.215 = GTGCGGTTCCGTCAAA-1

using 29 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.216 = GTGCGGTTCCGTCATC-1

using 164 reads

====================================================================================

graph has 226 edges initially, 4 edges after simplification

total ucounts = 19
nonsolo ucounts = 15[2, 3^2, 4, 6^3, 7, 9, 12, 15^2, 20^2, 32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.221 = GTGCGGTTCCTTGCCA-1

using 180 reads

====================================================================================

graph has 156 edges initially, 8 edges after simplification

total ucounts = 24
nonsolo ucounts = 18[2^2, 3, 4^3, 7^2, 9, 10^3, 11^2, 14, 15, 21, 30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.222 = GTGCGGTTCCTTGGTC-1

using 213 reads

====================================================================================

graph has 236 edges initially, 4 edges after simplification

total ucounts = 25
nonsolo ucounts = 16[2^2, 3^2, 4, 5, 7, 12, 13, 14, 16, 18, 19^2, 31, 36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.224 = GTGCGGTTCGAGAACG-1

using 93 reads

====================================================================================

graph has 94 edges initially, 2 edges after simplification

total ucounts = 14
nonsolo ucounts = 11[2^2, 3, 4^2, 7^2, 8, 9, 16, 28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.227 = GTGCGGTTCGCCAGCA-1

using 110 reads

====================================================================================

graph has 156 edges initially, 4 edges after simplification

total ucounts = 15
nonsolo ucounts = 13[2, 3^3, 4, 6, 7, 8, 11, 12, 13, 15, 21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.230 = GTGCGGTTCGCGTTTC-1

using 45932 reads

====================================================================================

graph has 19828 edges initially, 412 edges after simplification

total ucounts = 2518
nonsolo ucounts = 1937[2^234, 3^133, 4^132, 5^125, 6^129, 7^94, 8^102, 9^96, 10^60, 11^72, 12^53, 13^57, 14^51, 15^43, 16^44, 17^53, 18^53, 19^41, 20^39, 21^23, 22^36, 23^21, 24^30, 25^26, 26^12, 27^25, 28^17, 29^18, 30^13, 31^10, 32^6, 33^10, 34^9, 35^10, 36^7, 37^8, 38^3, 40, 41, 42^2, 43, 45, 46, 47, 48^2, 49, 50, 54^2, 55, 57^2, 64, 69, 71, 79, 82, 96, 124, 183, 201, 232, 351, 399, 903, 1019, 1210, 1324, 1353, 1463, 1479, 1513, 1838, 1858, 2031, 2474, 3086]
surviving nonsolo ucounts = 19[124, 183, 201, 232, 351, 399, 903, 1019, 1210, 1324, 1353, 1463, 1479, 1513, 1838, 1858, 2031, 2474, 3086]
ids = [622, 1909, 1214, 346, 1286, 2263, 2477, 1081, 309, 326, ...]

====================================================================================

UMI info for barcode GTGCGGTTCGCGTTTC-1 contig 1 = ACAGAAGTGG...
umi ACTATCTTTT = 1221 reads: -29X +3 -3XX +1 -3XX +2 -7XX +1 -3XX +339 invalidated
umi CGCATAATGG = 2047 reads: +391 validated
umi CGTCACTATA = 1314 reads: +391 validated
umi CTGCGTACCA = 1841 reads: +391 validated
umi CTTTTCCCGC = 1864 reads: +391 validated
umi GCTCTGCCCT = 1510 reads: +391 validated
umi GTAATTCGTA = 2484 reads: +391 validated
umi TACGGATCTT = 161 reads: -369 +4 -2XX +2 -1XX +1 -1XX +3 -2XX +1 -1XX +2 -2XX invalidated

UMI info for barcode GTGCGGTTCGCGTTTC-1 contig 2 = GTTCCCCTAT...
umi ACAAAGGTTT = 1468 reads: +394 validated
umi ACTGTCAGCC = 1285 reads: +394 validated
umi ACTTTTCTTA = 209 reads: -363X +2 -1X +3 -3X +5 -2X +1 -2X +1 -2XX +2 -1XX +1 -2XX +3 invalidated
umi ATTACTTTAA = 96 reads: -385X +2 -1XX +1 -2XX +3 invalidated
umi CACCCCGGGT = 2825 reads: -373 +1 -1XX +3 -1XX +2 -1XX +1 -1XX +2 -1XX +2 -2XX +1 -1XX +1 invalidated
umi CTAGAGGGTC = 1463 reads: +394 validated
umi CTCGCATAGC = 148 reads: -376 +1 -2X +2 -1X +1 -1XX +5 -2XX +3 invalidated
umi CTTCAATTCC = 309 reads: -376X +1 -1X +3 -1XX +1 -1XX +5 -2XX +1 -1XX +1 invalidated
umi TGCTCCATCA = 352 reads: -378 +3 -1XX +1 -1XX +5 -2XX +1 -1XX +1 invalidated
umi TTTACATCTC = 901 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=502]
0-69 ==> 0-69 on |478|TRAV20|5'UTR| [len=69] (mis=0)
69-406 ==> 0-337 on |479|TRAV20|L-REGION+V-REGION| [len=337] (mis=2)
405-460 ==> 0-55 on |406|TRAJ21|J-REGION| [len=55] (mis=2)
460-502 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 7 umis using 580 reads
cdr3 = CAVRGNFNKFYF at 393, score = 8 + 7
umis assigned: [309, 1030, 1114, 1249, 1326, 1565, 1702, 1909]
of which 8 are surviving nonsolos
reads assigned: 12219
start codons at 69, 78, 448
confident = true

TIG 2[bases=503]
0-57 ==> 0-57 on |647|TRBV6-1|5'UTR| [len=57] (mis=0)
57-401 ==> 0-344 on |648|TRBV6-1|L-REGION+V-REGION| [len=344] (mis=1)
411-451 ==> 8-48 on |537|TRBJ1-2|J-REGION| [len=48] (mis=0)
451-503 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 4 umis using 270 reads
cdr3 = CASSESSSGGYTF at 384, score = 9 + 9
umis assigned: [157, 326, 346, 622, 731, 1157, 1214, 1286, 2263, 2477]
of which 10 are surviving nonsolos
reads assigned: 8961
start codons at 15, 57, 115, 168, 192, 207, 234, 298, 307
confident = true

REJECT CONTIGS

TIG 1[bases=403]
5-312 ==> 23-330 on |501|TRAV35|L-REGION+V-REGION| [len=330] (mis=2)
312-372 ==> 0-60 on |441|TRAJ54|J-REGION| [len=60] (mis=1)
372-403 ==> 0-31 on |393|TRAC|C-REGION| [len=422] (mis=1)
cdr3 = CWAGIQGAQKLVF at 302, score = 3 + 9
umis assigned: [1081]
of which 1 are surviving nonsolos
reads assigned: 990
start codons at 15, 26, 66, 88, 96, 134, 208, 283
confident = false
VJ delta = 19
not full
not full
now this is a cell
paired!

GCCCCTAAACCTGAAGACTCAGCCACTTATCTCTGTGCTGTGCGCGGCAACTTCAACAAATTTTACTTTGGATCTGGGACCAAACTCAATGTAAAACCAA <==> TCGGCTGCTCCCTCCCAGACATCTGTGTACTTCTGTGCCAGCAGTGAATCGAGCTCGGGGGGCTACACCTTCGGTTCGGGGACCAGGTTAACCGTTGTAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.231 = GTGCGGTTCGGATGGA-1

using 186 reads

====================================================================================

graph has 142 edges initially, 6 edges after simplification

total ucounts = 20
nonsolo ucounts = 16[4^2, 5, 6, 7^2, 9, 11^2, 12^2, 13, 15, 17, 18, 31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.232 = GTGCGGTTCGGCCGAT-1

using 83 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[82]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.235 = GTGCGGTTCGTCTGCT-1

using 204 reads

====================================================================================

graph has 262 edges initially, 16 edges after simplification

total ucounts = 24
nonsolo ucounts = 17[2^4, 3, 4^2, 7^2, 8, 15, 16, 19, 20, 21, 32, 33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.237 = GTGCGGTTCGTTTATC-1

using 44 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[44]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.241 = GTGCGGTTCTATCCCG-1

using 147 reads

====================================================================================

graph has 189 edges initially, 4 edges after simplification

total ucounts = 23
nonsolo ucounts = 13[2, 3, 4, 5, 8, 9, 12, 13, 14^2, 15, 17, 21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.248 = GTGCGGTTCTGGCGAC-1

using 381 reads

====================================================================================

graph has 328 edges initially, 20 edges after simplification

total ucounts = 28
nonsolo ucounts = 21[2^5, 3^2, 4, 7^2, 9^2, 19, 21, 26, 35^2, 38, 40, 52, 56]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.254 = GTGCGGTTCTTTAGGG-1

using 81 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[80]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.263 = GTGCTTCAGACTTGAA-1

using 142 reads

====================================================================================

graph has 180 edges initially, 4 edges after simplification

total ucounts = 16
nonsolo ucounts = 12[2^2, 3^3, 5, 6, 8^2, 18, 27, 53]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.264 = GTGCTTCAGAGACTTA-1

using 27 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.265 = GTGCTTCAGAGCTGCA-1

using 8298 reads

====================================================================================

graph has 1214 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 5[2^2, 6, 7, 8277]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.267 = GTGCTTCAGATCCTGT-1

using 93 reads

====================================================================================

graph has 62 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[93]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.268 = GTGCTTCAGATCGATA-1

using 93 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[93]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.272 = GTGCTTCAGATGTGTA-1

using 73 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[73]
surviving nonsolo ucounts = 1[73]
ids = [0]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.274 = GTGCTTCAGCCACGTC-1

using 14976 reads

====================================================================================

graph has 8156 edges initially, 176 edges after simplification

total ucounts = 982
nonsolo ucounts = 719[2^131, 3^78, 4^61, 5^60, 6^39, 7^43, 8^36, 9^24, 10^21, 11^20, 12^14, 13^14, 14^17, 15^13, 16^11, 17^9, 18^7, 19^8, 20^8, 21^10, 22^6, 23^6, 24^9, 25^3, 26^5, 27^3, 28^2, 29^4, 30^2, 31^2, 32^4, 33^4, 34^4, 35^5, 36^5, 38^4, 39, 40, 41^2, 42, 43^2, 46, 48^2, 49, 51^2, 52^2, 54, 55, 56, 66, 70, 79, 80, 81^2, 95^2, 6976]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================

REJECT CONTIGS

TIG 1[bases=329]
1-35 ==> 293-327 on segment before TRAJ18 exon 1 [len=327] (mis=0)
35-101 ==> 0-66 on |402|TRAJ18|J-REGION| [len=66] (mis=0)
287-329 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
umis assigned: [181]
of which 0 are surviving nonsolos
reads assigned: 6892
start codons at 12, 125, 171, 247
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.275 = GTGCTTCAGCGTGAAC-1

using 50 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 3[2, 18, 26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.276 = GTGCTTCAGCTAAACA-1

using 44 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[44]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.283 = GTGCTTCAGGATGGTC-1

using 49 reads

====================================================================================

graph has 78 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 5[2, 3, 5^2, 32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.284 = GTGCTTCAGGATGTAT-1

using 27 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.287 = GTGCTTCAGGCATGTG-1

using 28 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.292 = GTGCTTCAGGTGCTTT-1

using 123 reads

====================================================================================

graph has 126 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 10[3^2, 6, 8, 9, 10, 12, 19, 22, 30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.298 = GTGCTTCAGTCGTACT-1

using 91 reads

====================================================================================

graph has 97 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 5[2, 4, 7, 23, 53]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.300 = GTGCTTCAGTGAACGC-1

using 18 reads

====================================================================================

graph has 6 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[2, 16]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.301 = GTGCTTCAGTGACTCT-1

using 140 reads

====================================================================================

graph has 68 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[139]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.302 = GTGCTTCAGTGATCGG-1

using 71 reads

====================================================================================

graph has 80 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 7[2^2, 5, 6, 12, 21^2]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.303 = GTGCTTCAGTGGCACA-1

using 43 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[43]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.305 = GTGCTTCAGTGGTAGC-1

using 72 reads

====================================================================================

graph has 56 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 3[7, 9, 52]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.306 = GTGCTTCAGTGTACTC-1

using 87 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[87]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.307 = GTGCTTCAGTGTGAAT-1

using 37 reads

====================================================================================

graph has 54 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 3[2, 12, 16]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.314 = GTGCTTCCAACGATGG-1

using 720 reads

====================================================================================

graph has 810 edges initially, 22 edges after simplification

total ucounts = 109
nonsolo ucounts = 69[2^11, 3^9, 4^2, 5^9, 6^7, 7^2, 8, 9^5, 10, 11, 12^2, 13^3, 14, 15, 16^2, 17^2, 18^2, 19, 21, 22^2, 28, 34, 44, 64]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.317 = GTGCTTCCAACTGGCC-1

using 44 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[43]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.319 = GTGCTTCCAAGAGGCT-1

using 51 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[51]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.321 = GTGCTTCCAAGCGATG-1

using 60 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[60]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.322 = GTGCTTCCAAGCGCTC-1

using 73 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[3, 69]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.323 = GTGCTTCCAAGCTGAG-1

using 6466 reads

====================================================================================

graph has 3035 edges initially, 86 edges after simplification

total ucounts = 383
nonsolo ucounts = 268[2^65, 3^26, 4^25, 5^20, 6^17, 7^17, 8^8, 9^14, 10^4, 11^4, 12^7, 13^2, 14^2, 15^5, 16^5, 18^2, 19^2, 20^3, 21^3, 22^4, 23, 24^4, 25^2, 26^6, 27^2, 28, 29, 31, 34, 36, 39, 41, 42, 45, 46, 49, 52, 57, 63, 155, 237, 293, 3214]
surviving nonsolo ucounts = 5[2, 46, 155, 237, 3214]
ids = [109, 341, 297, 320, 310]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.324 = GTGCTTCCAAGTAATG-1

using 106 reads

====================================================================================

graph has 38 edges initially, 4 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[21, 85]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.325 = GTGCTTCCAAGTCTGT-1

using 134 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[134]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.327 = GTGCTTCCAATAGAGT-1

using 125 reads

====================================================================================

graph has 124 edges initially, 10 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[15, 17, 18, 73]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.330 = GTGCTTCCAATGGTCT-1

using 214 reads

====================================================================================

graph has 174 edges initially, 6 edges after simplification

total ucounts = 21
nonsolo ucounts = 15[2, 4, 5^3, 7, 8^2, 9, 13, 16, 18, 25, 41, 42]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.332 = GTGCTTCCACATCCGG-1

using 139 reads

====================================================================================

graph has 104 edges initially, 4 edges after simplification

total ucounts = 13
nonsolo ucounts = 10[2^4, 3, 4, 6, 15, 48, 52]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.337 = GTGCTTCCACCAGTTA-1

using 45 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[45]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.340 = GTGCTTCCACCTGGTG-1

using 237 reads

====================================================================================

graph has 142 edges initially, 6 edges after simplification

total ucounts = 8
nonsolo ucounts = 7[5, 7, 8, 10^2, 48, 148]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.343 = GTGCTTCCACGCTTTC-1

using 79 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[4, 9, 64]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.345 = GTGCTTCCACGTTGGC-1

using 99 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[99]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.350 = GTGCTTCCAGACAGGT-1

using 70 reads

====================================================================================

graph has 54 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[70]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.354 = GTGCTTCCAGCGTCCA-1

using 15 reads

====================================================================================

graph has 4 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.357 = GTGCTTCCAGGACCCT-1

using 46 reads

====================================================================================

graph has 56 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 4[3, 7, 9, 26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.358 = GTGCTTCCAGGACGTA-1

using 15 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[3, 10]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.360 = GTGCTTCCAGGGATTG-1

using 27 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.362 = GTGCTTCCAGGTCGTC-1

using 67 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[67]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.364 = GTGCTTCCAGTATAAG-1

using 116 reads

====================================================================================

graph has 126 edges initially, 4 edges after simplification

total ucounts = 15
nonsolo ucounts = 11[2^2, 3^2, 5, 6, 9, 13, 18, 25, 26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.368 = GTGCTTCCATCCGGGT-1

using 6947 reads

====================================================================================

graph has 5987 edges initially, 154 edges after simplification

total ucounts = 862
nonsolo ucounts = 613[2^99, 3^70, 4^51, 5^42, 6^46, 7^47, 8^26, 9^26, 10^17, 11^16, 12^16, 13^17, 14^13, 15^10, 16^9, 17^10, 18^6, 19^7, 20^8, 21^4, 22^4, 23^5, 24^7, 25^2, 26^2, 27^5, 28^5, 29^2, 31, 32, 33^3, 34^3, 35, 37^2, 39^2, 40^2, 41^4, 42, 43^2, 44, 45^2, 46, 47, 48, 51, 52^3, 54, 56, 57, 60, 67, 70, 80, 114, 269]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.370 = GTGCTTCCATCTGGTA-1

using 70 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[4, 66]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.375 = GTGCTTCCATTAGGCT-1

using 26 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.378 = GTGCTTCGTAAACGCG-1

using 240 reads

====================================================================================

graph has 168 edges initially, 6 edges after simplification

total ucounts = 9
nonsolo ucounts = 6[6, 17, 32, 35, 65, 82]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.379 = GTGCTTCGTAAAGTCA-1

using 8396 reads

====================================================================================

graph has 4907 edges initially, 152 edges after simplification

total ucounts = 531
nonsolo ucounts = 404[2^67, 3^33, 4^29, 5^29, 6^21, 7^15, 8^24, 9^15, 10^7, 11^11, 12^5, 13^8, 14^8, 15^12, 16^7, 17^4, 18^9, 19^3, 20^4, 21^10, 22^6, 23^7, 24^5, 25^4, 26^3, 27^4, 28^3, 29^4, 31^7, 32^4, 33^2, 34^5, 36^3, 37, 38^2, 39^4, 42, 44, 45^3, 47, 49, 50^3, 54, 55, 70, 71, 79, 97, 290, 719, 2122]
surviving nonsolo ucounts = 2[719, 2122]
ids = [484, 135]

====================================================================================

UMI info for barcode GTGCTTCGTAAAGTCA-1 contig 1 = CTTTTCTCAG...
umi ATATCCTCTG = 6 reads: -385X +1 -2XX invalidated
umi ATCCCATTAA = 2126 reads: +388 validated
umi TGCGGCACAC = 669 reads: -368 +4 -1XX +2 -1XX +1 -2XX +1 -1XX +2 -2XX +1 -1XX +1 invalidated

GOOD CONTIGS

TIG 1[bases=471]
0-31 ==> 36-67 on |614|TRBV29-1|5'UTR| [len=67] (mis=0)
31-366 ==> 0-335 on |615|TRBV29-1|L-REGION+V-REGION| [len=335] (mis=3)
370-382 ==> 0-12 on |535|TRBD1|D-REGION| [len=12] (mis=1)
379-419 ==> 8-48 on |537|TRBJ1-2|J-REGION| [len=48] (mis=0)
419-471 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 1 umis using 136 reads
cdr3 = CSVDRTGTGGYTF at 352, score = 7 + 9
umis assigned: [124, 135, 484]
of which 2 are surviving nonsolos
reads assigned: 2773
start codons at 31, 166, 169, 245, 322
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.380 = GTGCTTCGTAACGTTC-1

using 49 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[49]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.382 = GTGCTTCGTAAGTTCC-1

using 73 reads

====================================================================================

graph has 58 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[73]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.386 = GTGCTTCGTAGCTGCC-1

using 29 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.387 = GTGCTTCGTATAAACG-1

using 17184 reads

====================================================================================

graph has 9310 edges initially, 302 edges after simplification

total ucounts = 906
nonsolo ucounts = 690[2^103, 3^57, 4^52, 5^31, 6^29, 7^30, 8^32, 9^29, 10^24, 11^20, 12^15, 13^16, 14^12, 15^17, 16^10, 17^17, 18^9, 19^18, 20^11, 21^9, 22^8, 23^10, 24^10, 25^5, 26^7, 27^6, 28^3, 29^3, 30^6, 31^6, 32, 33^5, 34^7, 35^4, 36^2, 38^3, 39^3, 40^5, 41^2, 42^4, 43^7, 44^2, 45, 46, 47^2, 48, 49, 50^3, 51^2, 52, 53^2, 54^2, 55^4, 57, 58^2, 59^2, 60^2, 62, 64, 67, 73, 81, 84, 88, 91, 95, 102, 1882, 2324, 2881]
surviving nonsolo ucounts = 3[1882, 2324, 2881]
ids = [257, 774, 591]

====================================================================================

UMI info for barcode GTGCTTCGTATAAACG-1 contig 1 = GGAGGCAGTG...
umi CACACTACAG = 1895 reads: +397 validated

UMI info for barcode GTGCTTCGTATAAACG-1 contig 2 = ATCTCAGACC...
umi CAATGGGTAC = 1 reads: -397X +3 invalidated
umi TCGCCCTGGC = 2319 reads: +400 validated

GOOD CONTIGS

TIG 1[bases=504]
0-55 ==> 23-78 on |597|TRBV20-1|5'UTR| [len=78] (mis=0)
55-390 ==> 0-335 on |598|TRBV20-1|L-REGION+V-REGION| [len=335] (mis=3)
402-452 ==> 0-50 on |543|TRBJ2-1|J-REGION| [len=50] (mis=5)
452-504 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 1 umis using 106 reads
cdr3 = CSARPGVAGEFNEQFF at 376, score = 7 + 8
umis assigned: [257]
of which 1 are surviving nonsolos
reads assigned: 1856
start codons at 55, 193, 229, 235, 248, 308, 410
confident = false

TIG 2[bases=472]
0-20 ==> 91-111 on |628|TRBV4-3|5'UTR| [len=111] (mis=0)
20-364 ==> 0-344 on |629|TRBV4-3|L-REGION+V-REGION| [len=344] (mis=0)
373-420 ==> 0-47 on |550|TRBJ2-7|J-REGION| [len=47] (mis=2)
420-472 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 1 umis using 136 reads
cdr3 = CASSQDEGGTYEQYF at 347, score = 10 + 8
umis assigned: [254, 774]
of which 1 are surviving nonsolos
reads assigned: 2289
start codons at 20, 74, 113, 119, 142, 170, 212, 277, 363
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.391 = GTGCTTCGTCAAACTC-1

using 106 reads

====================================================================================

graph has 90 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[105]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.397 = GTGCTTCGTCTCCATC-1

using 33 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.399 = GTGCTTCGTCTTCTCG-1

using 13 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[13]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.404 = GTGCTTCGTGCCTGTG-1

using 52 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 6[2, 3, 5, 6, 8, 26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.405 = GTGCTTCGTGCTCTTC-1

using 40 reads

====================================================================================

graph has 56 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 4[3, 4, 6, 22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.411 = GTGCTTCGTTCATGGT-1

using 61 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[61]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.412 = GTGCTTCGTTCCCTTG-1

using 76 reads

====================================================================================

graph has 41 edges initially, 4 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[23, 53]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.418 = GTGCTTCGTTTGACTG-1

using 93 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[92]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.420 = GTGCTTCTCAAAGACA-1

using 53 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[53]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.423 = GTGCTTCTCAATCTCT-1

using 45 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[2, 43]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.424 = GTGCTTCTCACCCGAG-1

using 160 reads

====================================================================================

graph has 168 edges initially, 2 edges after simplification

total ucounts = 18
nonsolo ucounts = 13[2, 3^2, 5^2, 8, 10, 11, 14, 17, 22, 27, 28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.431 = GTGCTTCTCATACGGT-1

using 398 reads

====================================================================================

graph has 314 edges initially, 12 edges after simplification

total ucounts = 19
nonsolo ucounts = 14[2, 3, 4, 8, 10, 12, 27^2, 28, 31, 44, 48, 65, 84]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.432 = GTGCTTCTCATGCTCC-1

using 65 reads

====================================================================================

graph has 76 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 6[5, 6, 7, 10, 12, 21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.433 = GTGCTTCTCATGTGGT-1

using 85 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[85]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.444 = GTGCTTCTCCGAACGC-1

using 156 reads

====================================================================================

graph has 140 edges initially, 6 edges after simplification

total ucounts = 9
nonsolo ucounts = 5[3, 12, 15, 52, 70]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.446 = GTGCTTCTCCGCGGTA-1

using 167 reads

====================================================================================

graph has 88 edges initially, 4 edges after simplification

total ucounts = 7
nonsolo ucounts = 6[2, 5, 23, 29, 40, 67]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.448 = GTGCTTCTCCTATTCA-1

using 106 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[106]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.451 = GTGCTTCTCCTGTAGA-1

using 70 reads

====================================================================================

graph has 62 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 6[2^2, 5^2, 6, 46]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.460 = GTGCTTCTCGCGGATC-1

using 107 reads

====================================================================================

graph has 120 edges initially, 4 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[5, 17, 38, 45]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.461 = GTGCTTCTCGCGTTTC-1

using 148 reads

====================================================================================

graph has 48 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[148]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.463 = GTGCTTCTCGGACAAG-1

using 27 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.464 = GTGCTTCTCGGCCGAT-1

using 93 reads

====================================================================================

graph has 56 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 5[2, 5, 9, 11, 66]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.466 = GTGCTTCTCGTAGGAG-1

using 965 reads

====================================================================================

graph has 432 edges initially, 4 edges after simplification

total ucounts = 11
nonsolo ucounts = 5[2, 3, 11, 30, 913]
surviving nonsolo ucounts = 1[913]
ids = [1]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.467 = GTGCTTCTCGTCACGG-1

using 112 reads

====================================================================================

graph has 116 edges initially, 4 edges after simplification

total ucounts = 16
nonsolo ucounts = 13[2^6, 4, 7, 8, 9, 16, 17, 36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.477 = GTGCTTCTCTCACATT-1

using 12 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[12]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.478 = GTGCTTCTCTCCAACC-1

using 62 reads

====================================================================================

graph has 92 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 7[2, 3, 7, 8^2, 9, 21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.479 = GTGCTTCTCTCCAGGG-1

using 28 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.482 = GTGCTTCTCTCGTATT-1

using 108 reads

====================================================================================

graph has 66 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[3, 4, 8, 91]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.483 = GTGCTTCTCTCTAGGA-1

using 19 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.486 = GTGCTTCTCTCTTGAT-1

using 11058 reads

====================================================================================

graph has 9055 edges initially, 316 edges after simplification

total ucounts = 1268
nonsolo ucounts = 970[2^137, 3^99, 4^80, 5^61, 6^52, 7^50, 8^46, 9^44, 10^31, 11^28, 12^35, 13^33, 14^23, 15^29, 16^15, 17^20, 18^13, 19^13, 20^21, 21^9, 22^12, 23^15, 24^6, 25^11, 26^13, 27^9, 28^6, 29^6, 30^2, 31^4, 32^3, 34^5, 36^5, 37^4, 38, 39^3, 40^3, 41^4, 42, 44, 45, 46, 48^2, 51^2, 52, 56^2, 57, 58^2, 59, 60^2, 91, 93]
surviving nonsolo ucounts = 1[36]
ids = [873]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.489 = GTGCTTCTCTGCTTGC-1

using 60 reads

====================================================================================

graph has 6 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[60]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.490 = GTGCTTCTCTGGCGTG-1

using 119 reads

====================================================================================

graph has 60 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[119]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.499 = GTGCTTCTCTTGAGAC-1

using 2721 reads

====================================================================================

graph has 2294 edges initially, 80 edges after simplification

total ucounts = 498
nonsolo ucounts = 282[2^70, 3^28, 4^35, 5^21, 6^10, 7^9, 8^15, 9^14, 10^11, 11^5, 12^6, 13^4, 14^3, 15, 16^8, 17^3, 18^2, 19^5, 20^3, 21^3, 23^5, 24^2, 26, 27, 28^3, 29, 30, 34^2, 36, 37, 39, 40^2, 41, 47, 54, 57, 69]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.500 = GTGCTTCTCTTGTATC-1

using 29 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.503 = GTGCTTCTCTTTAGGG-1

using 55 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[55]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.504 = GTGGGTCAGAAACGAG-1

using 19 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 1[15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.510 = GTGGGTCAGAGACTAT-1

using 325 reads

====================================================================================

graph has 280 edges initially, 12 edges after simplification

total ucounts = 31
nonsolo ucounts = 26[2^3, 3^2, 4^2, 6^2, 7, 8^2, 9^2, 10^2, 11, 13, 15^3, 20, 24, 29, 38, 47]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.511 = GTGGGTCAGAGATGAG-1

using 34 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.518 = GTGGGTCAGATATGGT-1

using 17790 reads

====================================================================================

graph has 12354 edges initially, 298 edges after simplification

total ucounts = 1645
nonsolo ucounts = 1169[2^189, 3^137, 4^125, 5^79, 6^60, 7^61, 8^66, 9^49, 10^38, 11^37, 12^36, 13^31, 14^22, 15^18, 16^19, 17^22, 18^13, 19^16, 20^17, 21^17, 22^19, 23^7, 24^8, 25^5, 26^11, 27^9, 28^5, 29^3, 30^5, 31^4, 32^6, 33^3, 34^2, 35^5, 39, 40, 42^4, 45, 46, 48, 51, 52, 53^2, 56, 57^2, 61, 62^2, 63^2, 91, 94, 955, 5085]
surviving nonsolo ucounts = 2[955, 5085]
ids = [1516, 1126]

====================================================================================

REJECT CONTIGS

TIG 1[bases=310]
5-59 ==> 32-86 on segment before TRBJ2-2P exon 1 [len=86] (mis=0)
59-105 ==> 0-46 on |545|TRBJ2-2P|J-REGION| [len=46] (mis=0)
105-209 ==> 0-104 on segment before TRBJ2-3 exon 1 [len=104] (mis=0)
258-310 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
umis assigned: [1126, 1516]
of which 2 are surviving nonsolos
reads assigned: 5578
start codons at 16, 119
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.522 = GTGGGTCAGATGCCAG-1

using 44 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[9, 35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.528 = GTGGGTCAGCATCATC-1

using 86 reads

====================================================================================

graph has 106 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 4[2, 9, 31, 39]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.540 = GTGGGTCAGCTGCAAG-1

using 45 reads

====================================================================================

graph has 60 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[45]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.543 = GTGGGTCAGCTGTCTA-1

using 47 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[47]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.545 = GTGGGTCAGGACGAAA-1

using 6395 reads

====================================================================================

graph has 4705 edges initially, 112 edges after simplification

total ucounts = 607
nonsolo ucounts = 428[2^78, 3^63, 4^45, 5^31, 6^31, 7^22, 8^16, 9^13, 10^7, 11^11, 12^17, 13^13, 14^6, 15^7, 16^5, 17^2, 18^11, 19^3, 20^5, 21^2, 22^2, 23^3, 24^2, 25^2, 26, 27, 28, 29, 30^2, 31, 33^3, 37, 39, 43, 44^2, 46^2, 48, 52, 58, 59, 64, 67, 79, 99, 100, 102, 108, 142, 751, 1148]
surviving nonsolo ucounts = 2[751, 1148]
ids = [425, 554]

====================================================================================

UMI info for barcode GTGGGTCAGGACGAAA-1 contig 1 = AGAATGACGC...
umi GTCCATTTTA = 752 reads: +394 validated
umi TTAACCGCTT = 1141 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=526]
0-80 ==> 0-80 on |651|TRBV6-2|5'UTR| [len=80] (mis=0)
80-424 ==> 0-344 on |652|TRBV6-2|L-REGION+V-REGION| [len=344] (mis=4)
433-474 ==> 7-48 on |548|TRBJ2-5|J-REGION| [len=48] (mis=0)
474-526 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 2 umis using 110 reads
cdr3 = CASSSGLAGTQYF at 407, score = 9 + 7
umis assigned: [425, 554]
of which 2 are surviving nonsolos
reads assigned: 1864
start codons at 3, 38, 80, 138, 191, 215, 222, 230, 257, 321, 330
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.550 = GTGGGTCAGGCATGGT-1

using 268 reads

====================================================================================

graph has 242 edges initially, 6 edges after simplification

total ucounts = 11
nonsolo ucounts = 9[9^2, 12, 19, 20, 36, 41, 44, 76]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.552 = GTGGGTCAGGCCCTCA-1

using 260 reads

====================================================================================

graph has 204 edges initially, 6 edges after simplification

total ucounts = 20
nonsolo ucounts = 13[2^2, 4^2, 7^2, 8, 9, 11, 38^2, 57, 66]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.555 = GTGGGTCAGGCTCTTA-1

using 49 reads

====================================================================================

graph has 68 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 4[3, 7, 9, 30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.558 = GTGGGTCAGTAGGTGC-1

using 36 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.562 = GTGGGTCAGTCTCCTC-1

using 54 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[5, 49]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.563 = GTGGGTCAGTGAACAT-1

using 5074 reads

====================================================================================

graph has 1215 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[2, 5070]
surviving nonsolo ucounts = 1[5070]
ids = [0]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.566 = GTGGGTCAGTTAGGTA-1

using 755 reads

====================================================================================

graph has 322 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[755]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.577 = GTGGGTCCAAGCCGTC-1

using 39 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[39]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.579 = GTGGGTCCAAGCTGTT-1

using 49 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[19, 29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.580 = GTGGGTCCAAGGACTG-1

using 39 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[39]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.586 = GTGGGTCCAAGTTAAG-1

using 5567 reads

====================================================================================

graph has 4884 edges initially, 122 edges after simplification

total ucounts = 729
nonsolo ucounts = 502[2^86, 3^60, 4^39, 5^43, 6^40, 7^21, 8^18, 9^19, 10^16, 11^24, 12^7, 13^16, 14^7, 15^10, 16^6, 17^10, 18^7, 19^4, 20, 21^9, 22^7, 23^6, 24^6, 25^2, 27^2, 29, 30^3, 32^2, 33^2, 35^2, 36^3, 38, 39, 41, 42^2, 43^3, 46, 48, 49, 50^2, 51, 54^2, 55, 59, 63, 65, 70, 91, 171]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.591 = GTGGGTCCAATTGCTG-1

using 77 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[2, 75]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.592 = GTGGGTCCACAAGTAA-1

using 75 reads

====================================================================================

graph has 62 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 5[2^2, 8, 15, 45]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.593 = GTGGGTCCACACGCTG-1

using 30 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.597 = GTGGGTCCACATGACT-1

using 40 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[40]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.598 = GTGGGTCCACATTCGA-1

using 82 reads

====================================================================================

graph has 100 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[82]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.603 = GTGGGTCCACGAAACG-1

using 48 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[6, 40]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.615 = GTGGGTCCAGATTGCT-1

using 71 reads

====================================================================================

graph has 56 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[71]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.617 = GTGGGTCCAGCCTTTC-1

using 53 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[53]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.619 = GTGGGTCCAGCGATCC-1

using 449 reads

====================================================================================

graph has 360 edges initially, 14 edges after simplification

total ucounts = 25
nonsolo ucounts = 19[3, 6^2, 7, 8^4, 13, 14, 20, 23, 33, 35, 37, 41, 49, 62^2]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.623 = GTGGGTCCAGTGAGTG-1

using 40 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 2[2, 35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.627 = GTGGGTCCATACAGCT-1

using 487 reads

====================================================================================

graph has 300 edges initially, 14 edges after simplification

total ucounts = 23
nonsolo ucounts = 18[2^2, 3^3, 5, 8, 9, 10, 13, 18, 28, 40, 51, 55, 60, 77, 95]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.628 = GTGGGTCCATACCATG-1

using 48 reads

====================================================================================

graph has 60 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 4[3, 6, 14, 20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.630 = GTGGGTCCATACTACG-1

using 70 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[70]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.646 = GTGGGTCCATTTGCTT-1

using 41 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[41]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.651 = GTGGGTCGTAGCCTAT-1

using 56 reads

====================================================================================

graph has 58 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[56]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.655 = GTGGGTCGTATCTGCA-1

using 26 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.657 = GTGGGTCGTCAATGTC-1

using 33 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.663 = GTGGGTCGTCCGTGAC-1

using 705 reads

====================================================================================

graph has 621 edges initially, 32 edges after simplification

total ucounts = 48
nonsolo ucounts = 36[2^7, 3^3, 4^2, 5^2, 6^3, 7^2, 8, 11, 12, 16, 20^2, 24^2, 41, 45, 47^3, 49, 57, 71, 81]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.680 = GTGGGTCGTTCAGCGC-1

using 295 reads

====================================================================================

graph has 244 edges initially, 10 edges after simplification

total ucounts = 11
nonsolo ucounts = 7[4^2, 24, 47, 49, 73, 90]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.682 = GTGGGTCGTTCCATGA-1

using 86 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[86]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.689 = GTGGGTCTCAACGGGA-1

using 49 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[49]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.691 = GTGGGTCTCAAGAAGT-1

using 19 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.695 = GTGGGTCTCACAGGCC-1

using 73 reads

====================================================================================

graph has 68 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 4[4, 12, 21, 36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.697 = GTGGGTCTCACCACCT-1

using 21 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.698 = GTGGGTCTCACCCGAG-1

using 13 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[4, 7]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.699 = GTGGGTCTCACCGGGT-1

using 49 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[49]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.701 = GTGGGTCTCAGAGGTG-1

using 86 reads

====================================================================================

graph has 98 edges initially, 2 edges after simplification

total ucounts = 18
nonsolo ucounts = 10[2^4, 4, 5, 6, 7, 16, 32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.704 = GTGGGTCTCAGCTTAG-1

using 36 reads

====================================================================================

graph has 52 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 6[2^2, 3, 5, 7, 15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.708 = GTGGGTCTCATCTGTT-1

using 2447 reads

====================================================================================

graph has 2185 edges initially, 64 edges after simplification

total ucounts = 374
nonsolo ucounts = 252[2^43, 3^50, 4^25, 5^19, 6^10, 7^9, 8^6, 9^13, 10^5, 11^13, 12^4, 13^3, 14^6, 15^2, 16^5, 17^4, 18, 19, 20^4, 21^3, 22^2, 23, 25^2, 26, 27^2, 28^3, 29^2, 30^2, 32^3, 36^2, 38, 42, 45, 55, 63, 74]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.724 = GTGGGTCTCCGCGCAA-1

using 53 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[8, 45]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.725 = GTGGGTCTCCGTAGGC-1

using 26 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.730 = GTGGGTCTCCTGTACC-1

using 1522 reads

====================================================================================

graph has 482 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 3[2^2, 1514]
surviving nonsolo ucounts = 1[1514]
ids = [1]

====================================================================================

UMI info for barcode GTGGGTCTCCTGTACC-1 contig 1 = GATATTCCGA...
umi CGACCGTGAA = 1523 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=483]
53-389 ==> 0-336 on |512|TRAV41|L-REGION+V-REGION| [len=336] (mis=2)
385-441 ==> 0-56 on |436|TRAJ49|J-REGION| [len=56] (mis=4)
441-483 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 1 umis using 85 reads
cdr3 = CAVLTGNQFYF at 377, score = 6 + 7
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 1498
start codons at 53, 123, 260, 285
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.735 = GTGGGTCTCGCCATAA-1

using 17493 reads

====================================================================================

graph has 6812 edges initially, 140 edges after simplification

total ucounts = 821
nonsolo ucounts = 534[2^129, 3^67, 4^63, 5^39, 6^28, 7^22, 8^27, 9^20, 10^14, 11^15, 12^11, 13^10, 14^9, 15^4, 16^8, 17^7, 18^2, 19^5, 20^6, 21^2, 22^4, 23^4, 24^4, 25^3, 26^3, 27^2, 28^2, 30, 31, 32^3, 33^2, 34^2, 37^2, 38, 42, 43, 44, 49^2, 265, 962, 1451, 1541, 2108, 2890, 3903]
surviving nonsolo ucounts = 7[265, 962, 1451, 1541, 2108, 2890, 3903]
ids = [454, 439, 666, 743, 754, 795, 443]

====================================================================================

UMI info for barcode GTGGGTCTCGCCATAA-1 contig 1 = AGACACAGTG...
umi GACCACCCTG = 963 reads: +391 validated
umi GACGTACCCC = 3540 reads: -349 +1 -1XX +2 -1XX +5 -1XX +2 -1XX +2 -1XX +8 -1XX +5 -4XX +7 invalidated
umi GATCTTTTTC = 246 reads: -364 +7 -1X +2 -1X +6 -3XX +5 -1XX +1 invalidated
umi TCTGTGGGTG = 1447 reads: +391 validated
umi TTATTAAGGC = 1543 reads: +391 validated
umi TTCCTACTGC = 2121 reads: +391 validated
umi TTTCACTTGG = 2898 reads: +391 validated

GOOD CONTIGS

TIG 1[bases=472]
0-29 ==> 280-309 on |630|TRBV5-1|5'UTR| [len=309] (mis=0)
29-372 ==> 0-343 on |631|TRBV5-1|L-REGION+V-REGION| [len=343] (mis=0)
372-420 ==> 0-48 on |548|TRBJ2-5|J-REGION| [len=48] (mis=5)
420-472 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 5 umis using 509 reads
cdr3 = CASSLGGETQYF at 356, score = 9 + 7
umis assigned: [439, 443, 454, 666, 743, 754, 795]
of which 7 are surviving nonsolos
reads assigned: 12562
start codons at 29, 311, 315
confident = true
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.748 = GTGGGTCTCTCATTCA-1

using 60 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[60]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.749 = GTGGGTCTCTCCGGTT-1

using 118 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[118]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.750 = GTGGGTCTCTCGATGA-1

using 60 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[60]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.765 = GTGTGCGAGACTACAA-1

using 38 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.768 = GTGTGCGAGATCCCAT-1

using 276 reads

====================================================================================

graph has 283 edges initially, 10 edges after simplification

total ucounts = 17
nonsolo ucounts = 12[2, 8, 10, 12, 14, 15, 19, 20, 24, 33, 41, 73]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.771 = GTGTGCGAGATGCCTT-1

using 144 reads

====================================================================================

graph has 122 edges initially, 6 edges after simplification

total ucounts = 12
nonsolo ucounts = 9[2, 4, 7, 8, 11, 17, 22, 30, 40]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.773 = GTGTGCGAGCAGCGTA-1

using 31 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.774 = GTGTGCGAGCCACGCT-1

using 59 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 5[2, 4, 15, 16, 20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.783 = GTGTGCGAGGACACCA-1

using 165 reads

====================================================================================

graph has 194 edges initially, 10 edges after simplification

total ucounts = 15
nonsolo ucounts = 10[2^2, 5, 8, 11^2, 12, 18, 34, 57]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.786 = GTGTGCGAGGCGACAT-1

using 53 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[53]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.788 = GTGTGCGAGGTAGCTG-1

using 62 reads

====================================================================================

graph has 98 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 7[2, 3, 5^2, 6^2, 32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.789 = GTGTGCGAGGTCGGAT-1

using 57 reads

====================================================================================

graph has 66 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 5[4, 8, 9, 12, 24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.791 = GTGTGCGAGGTGCTTT-1

using 63 reads

====================================================================================

graph has 96 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 7[2, 3^2, 4, 11, 16, 21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.795 = GTGTGCGAGTATTGGA-1

using 119 reads

====================================================================================

graph has 134 edges initially, 4 edges after simplification

total ucounts = 20
nonsolo ucounts = 12[2^2, 3, 4^3, 7, 9, 11, 16, 24, 25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.796 = GTGTGCGAGTCGATAA-1

using 60 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[60]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.799 = GTGTGCGAGTGATCGG-1

using 48520 reads

====================================================================================

graph has 11978 edges initially, 162 edges after simplification

total ucounts = 435
nonsolo ucounts = 282[2^54, 3^34, 4^28, 5^18, 6^11, 7^6, 8^10, 9^6, 10^11, 11^9, 12^8, 13^5, 14, 15^3, 16^8, 17^3, 18^3, 19^2, 20^4, 21, 22^2, 24^2, 25, 26^2, 28^3, 31, 33, 34, 35, 36, 37, 39, 40, 41, 42, 43^3, 44, 45, 47, 50, 54^2, 56, 59, 68, 72, 77, 83, 99, 103, 154, 704, 829, 911, 1056, 1157, 1188, 1321, 1610, 1839, 2149, 2233, 2299, 2308, 2703, 2788, 2861, 3158, 6085, 7898]
surviving nonsolo ucounts = 18[829, 911, 1056, 1157, 1188, 1321, 1610, 1839, 2149, 2233, 2299, 2308, 2703, 2788, 2861, 3158, 6085, 7898]
ids = [106, 370, 28, 119, 318, 120, 195, 238, 123, 71, ...]

====================================================================================

UMI info for barcode GTGTGCGAGTGATCGG-1 contig 1 = CTCACAGTGA...
umi ACGCATTTGC = 1016 reads: -385 +1 -1XX +3 -1XX +4 -1XX +2 -1XX +3 -3XX +1 invalidated
umi AGCAGTCACT = 2241 reads: +406 validated
umi ATATAACGGG = 867 reads: +139 -6XX +3 -5XX +1 -1XX +1 -3XX +1 -5XX +1 -2X +1 -57X +1 -1X +1 -2X +1 -4XX +2 -1XX +2 -4XX +1 -7XX +2 -1XX +150 invalidated
umi ATTAGGGACC = 1174 reads: +406 validated
umi ATTATAGGCA = 1309 reads: +406 validated
umi ATTCCTAGCG = 717 reads: -387X +3 -1XX +4 -1XX +2 -1XX +3 -3XX +1 invalidated
umi ATTCGTTTAT = 5095 reads: -327X +1 -4XX +1 -5XX +1 -5XX +4 -2XX +2 -2XX +1 -2XX +1 -1XX +45 -1XX +1 invalidated
umi CACTGGGCTT = 8154 reads: +122 -4XX +1 -4XX +1 -7XX +3 -6XX +1 -5XX +1 -73X +3 -2XX +1 -7XX +1 -2XX +2 -2XX +2 -6XX +150 invalidated
umi CGTACAGTCG = 1621 reads: +406 validated
umi CTTTCTAGCT = 1834 reads: +406 validated
umi GGTCACACAC = 2794 reads: +406 validated
umi GGTCCACCGC = 3182 reads: -43 +3 -1XX +359 invalidated
umi GTGTCTCTTA = 1186 reads: +406 validated
umi GTTGGGGGGT = 2537 reads: -327X +1 -4XX +1 -5XX +1 -5XX +4 -2XX +2 -2XX +1 -2XX +1 -1XX +47 invalidated
umi TTGAGGTAGC = 2316 reads: +406 validated

UMI info for barcode GTGTGCGAGTGATCGG-1 contig 2 = TGGGGAAGTA...
umi ACACACCATG = 1053 reads: +394 validated
umi TCTGTTTATG = 921 reads: +394 validated
umi TTTGCATCCT = 2288 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=508]
0-50 ==> 0-50 on |678|TRBV7-3|5'UTR| [len=50] (mis=0)
50-397 ==> 0-347 on |679|TRBV7-3|L-REGION+V-REGION| [len=347] (mis=4)
407-456 ==> 0-49 on |546|TRBJ2-3|J-REGION| [len=49] (mis=1)
456-508 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 11 umis using 1958 reads
cdr3 = CASSFLSGAITDTQYF at 380, score = 8 + 8
umis assigned: [49, 71, 106, 119, 120, 123, 128, 145, 195, 238] and 5 others
of which 15 are surviving nonsolos
reads assigned: 35218
start codons at 50, 159, 273
confident = true

TIG 2[bases=616]
180-516 ==> 0-336 on |512|TRAV41|L-REGION+V-REGION| [len=336] (mis=1)
520-574 ==> 9-63 on |444|TRAJ58|J-REGION| [len=63] (mis=0)
574-616 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 3 umis using 181 reads
cdr3 = CAVRGASGSRLTF at 504, score = 6 + 9
umis assigned: [28, 370, 429]
of which 3 are surviving nonsolos
reads assigned: 4206
start codons at 19, 51, 85, 180, 250, 387, 412
confident = true
now this is a cell
paired!

TCCCATCCCAGAGACTCTGCCGTCTACATCTGTGCTGTCAGGGGAGCCAGTGGCTCTAGGTTGACCTTTGGGGAAGGAACACAGCTCACAGTGAATCCTG <==> CGGGGGGACTCAGCCGTGTATCTCTGTGCCAGCAGTTTCCTCTCGGGGGCCATCACAGATACGCAGTATTTTGGCCCAGGCACCCGGCTGACAGTGCTCG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.800 = GTGTGCGAGTGCCAGA-1

using 1706 reads

====================================================================================

graph has 1671 edges initially, 50 edges after simplification

total ucounts = 186
nonsolo ucounts = 136[2^30, 3^18, 4^11, 5^8, 6^5, 7^5, 8^6, 9^7, 10^4, 11^5, 13^5, 14, 15, 16^2, 17, 18, 19, 20, 21, 22, 24^2, 27^2, 30, 35^2, 37, 39, 40, 42, 44^2, 46, 49^2, 50, 52, 57, 58, 59, 76]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.806 = GTGTGCGAGTTGCAGG-1

using 15338 reads

====================================================================================

graph has 7122 edges initially, 114 edges after simplification

total ucounts = 1724
nonsolo ucounts = 1341[2^162, 3^81, 4^76, 5^73, 6^72, 7^66, 8^74, 9^74, 10^63, 11^72, 12^66, 13^63, 14^66, 15^51, 16^53, 17^41, 18^33, 19^15, 20^19, 21^18, 22^14, 23^7, 24^12, 25^7, 26, 27^5, 28^4, 29^4, 31, 32, 33^3, 34, 35^3, 36, 37, 38, 39, 41, 42^5, 43^2, 45, 46^4, 47^4, 48^2, 49, 50, 53, 54, 55^2, 59^2, 60^2, 61, 67, 68, 69, 73, 74, 75]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.816 = GTGTGCGCACATTAGC-1

using 239 reads

====================================================================================

graph has 186 edges initially, 12 edges after simplification

total ucounts = 12
nonsolo ucounts = 10[3, 5^2, 8, 9, 10, 36, 38, 48, 75]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.821 = GTGTGCGCACGACTCG-1

using 30 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.827 = GTGTGCGCAGATGAGC-1

using 95 reads

====================================================================================

graph has 59 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[95]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.829 = GTGTGCGCAGCGATCC-1

using 47 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 4[4^2, 7, 27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.830 = GTGTGCGCAGCTCGAC-1

using 61 reads

====================================================================================

graph has 88 edges initially, 2 edges after simplification

total ucounts = 14
nonsolo ucounts = 8[2^2, 3^2, 4, 13^2, 15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.831 = GTGTGCGCAGGCAGTA-1

using 20 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.836 = GTGTGCGCAGTTAACC-1

using 31 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.841 = GTGTGCGCATCTCGCT-1

using 207 reads

====================================================================================

graph has 98 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[32, 175]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.842 = GTGTGCGCATCTGGTA-1

using 2396 reads

====================================================================================

graph has 596 edges initially, 4 edges after simplification

total ucounts = 19
nonsolo ucounts = 14[2^2, 4^3, 7^2, 11, 12, 13^2, 29^2, 2254]
surviving nonsolo ucounts = 1[2254]
ids = [4]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.844 = GTGTGCGCATGGAATA-1

using 17 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[17]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.846 = GTGTGCGCATTGGTAC-1

using 56 reads

====================================================================================

graph has 70 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 7[2, 4, 5^2, 8, 9, 19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.848 = GTGTGCGGTAATTGGA-1

using 106 reads

====================================================================================

graph has 110 edges initially, 4 edges after simplification

total ucounts = 7
nonsolo ucounts = 5[11^2, 14, 28, 40]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.849 = GTGTGCGGTACAGCAG-1

using 40 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 4[2^2, 3, 30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.850 = GTGTGCGGTACAGTGG-1

using 84 reads

====================================================================================

graph has 52 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[9, 75]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.851 = GTGTGCGGTACATCCA-1

using 46 reads

====================================================================================

graph has 66 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 5[2^2, 10, 11, 20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.862 = GTGTGCGGTCCATGAT-1

using 24 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.864 = GTGTGCGGTCCGTGAC-1

using 41 reads

====================================================================================

graph has 88 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[41]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.865 = GTGTGCGGTCGCATAT-1

using 45 reads

====================================================================================

graph has 74 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[2, 3, 39]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.866 = GTGTGCGGTCGTGGCT-1

using 29 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.867 = GTGTGCGGTCTAGCGC-1

using 139 reads

====================================================================================

graph has 138 edges initially, 4 edges after simplification

total ucounts = 14
nonsolo ucounts = 10[2^3, 4, 7, 9, 10, 22, 36, 41]
surviving nonsolo ucounts = 1[41]
ids = [12]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.870 = GTGTGCGGTCTGCGGT-1

using 53 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 6[3^2, 4, 5, 8, 29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.872 = GTGTGCGGTGAGTATA-1

using 61 reads

====================================================================================

graph has 66 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 8[2^3, 6^2, 9^2, 23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.873 = GTGTGCGGTGAGTGAC-1

using 123 reads

====================================================================================

graph has 186 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[123]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.874 = GTGTGCGGTGATAAGT-1

using 18 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.875 = GTGTGCGGTGCAGACA-1

using 70 reads

====================================================================================

graph has 76 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 6[2, 6, 9, 13, 14, 24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.877 = GTGTGCGGTGGAAAGA-1

using 226 reads

====================================================================================

graph has 134 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 8[2, 3, 6, 15, 18, 46, 51, 84]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.889 = GTGTGCGGTTGAGTTC-1

using 99 reads

====================================================================================

graph has 136 edges initially, 2 edges after simplification

total ucounts = 14
nonsolo ucounts = 11[2^3, 4, 5, 7, 11^3, 16, 25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.892 = GTGTGCGGTTTGACTG-1

using 32 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.898 = GTGTGCGTCAGCACAT-1

using 123 reads

====================================================================================

graph has 128 edges initially, 6 edges after simplification

total ucounts = 10
nonsolo ucounts = 6[2^2, 3, 17, 30, 65]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.901 = GTGTGCGTCATGCATG-1

using 83 reads

====================================================================================

graph has 100 edges initially, 4 edges after simplification

total ucounts = 10
nonsolo ucounts = 8[2, 3, 4, 5^3, 11, 46]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.904 = GTGTGCGTCCAAGTAC-1

using 55 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[55]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.907 = GTGTGCGTCCCTAATT-1

using 50 reads

====================================================================================

graph has 71 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[50]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.913 = GTGTGCGTCGCTTGTC-1

using 3684 reads

====================================================================================

graph has 1952 edges initially, 28 edges after simplification

total ucounts = 326
nonsolo ucounts = 187[2^38, 3^21, 4^13, 5^16, 6^18, 7^10, 8^4, 9^13, 10^3, 11^5, 12^7, 13^6, 14^2, 15^2, 16^3, 17, 18^3, 19, 20^3, 21, 22, 23^3, 25, 27^2, 28, 29, 30, 31^2, 32, 37, 44, 993, 998]
surviving nonsolo ucounts = 2[993, 998]
ids = [303, 246]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.917 = GTGTGCGTCTACTCAT-1

using 26 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.919 = GTGTGCGTCTCAAGTG-1

using 73 reads

====================================================================================

graph has 106 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 5[3, 11, 16, 20, 21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.922 = GTGTGCGTCTCGTTTA-1

using 59 reads

====================================================================================

graph has 58 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 8[2, 3^2, 5, 6, 7, 14, 18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.923 = GTGTGCGTCTCTGTCG-1

using 269 reads

====================================================================================

graph has 320 edges initially, 18 edges after simplification

total ucounts = 22
nonsolo ucounts = 17[2^2, 3, 4, 6^3, 8, 9, 13, 14, 16, 17^2, 20, 45, 76]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.924 = GTGTGCGTCTGCCAGG-1

using 100 reads

====================================================================================

graph has 104 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 9[2, 3^2, 4^2, 9, 11, 12, 50]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.931 = GTGTTAGAGAACAACT-1

using 2819 reads

====================================================================================

graph has 1060 edges initially, 12 edges after simplification

total ucounts = 18
nonsolo ucounts = 12[2^2, 5, 6, 8^2, 10, 14, 24, 45, 1305, 1384]
surviving nonsolo ucounts = 2[1305, 1384]
ids = [6, 3]

====================================================================================

UMI info for barcode GTGTTAGAGAACAACT-1 contig 1 = GTCATCCCTC...
umi ATGAACCGTT = 1444 reads: +391 validated
umi CGTGACTCAA = 1310 reads: +391 validated

GOOD CONTIGS

TIG 1[bases=521]
0-78 ==> 0-78 on |597|TRBV20-1|5'UTR| [len=78] (mis=0)
78-413 ==> 0-335 on |598|TRBV20-1|L-REGION+V-REGION| [len=335] (mis=1)
421-469 ==> 0-48 on |548|TRBJ2-5|J-REGION| [len=48] (mis=2)
469-521 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 2 umis using 112 reads
cdr3 = CSARHPEEEETQYF at 399, score = 7 + 7
umis assigned: [3, 6]
of which 2 are surviving nonsolos
reads assigned: 2650
start codons at 21, 78, 216, 252, 258, 271, 331
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.933 = GTGTTAGAGAAGGGTA-1

using 63 reads

====================================================================================

graph has 50 edges initially, 4 edges after simplification

total ucounts = 4
nonsolo ucounts = 4[2, 10, 22, 29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.936 = GTGTTAGAGACAGGCT-1

using 14 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[2, 12]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.938 = GTGTTAGAGACTACAA-1

using 37 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.939 = GTGTTAGAGACTAGAT-1

using 125 reads

====================================================================================

graph has 72 edges initially, 4 edges after simplification

total ucounts = 9
nonsolo ucounts = 7[5, 9, 16^2, 23, 25, 29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.944 = GTGTTAGAGATCCTGT-1

using 38 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.950 = GTGTTAGAGCATCATC-1

using 153 reads

====================================================================================

graph has 104 edges initially, 4 edges after simplification

total ucounts = 23
nonsolo ucounts = 19[2^2, 3^4, 4, 5, 6^3, 9^2, 11, 12^2, 13, 16, 24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 72.951 = GTGTTAGAGCCAACAG-1

using 19488 reads

====================================================================================

graph has 6698 edges initially, 128 edges after simplification

total ucounts = 623
nonsolo ucounts = 462[2^68, 3^43, 4^49, 5^32, 6^24, 7^29, 8^28, 9^14, 10^17, 11^15, 12^15, 13^11, 14^9, 15^7, 16^11, 17^9, 18^6, 19^5, 20^4, 21^3, 22^4, 23^3, 24^3, 25^6, 26^2, 27^2, 28^2, 29^2, 30^4, 31^4, 33^5, 34, 35, 37^3, 38, 39^4, 43, 44, 45, 46, 49, 54^2, 55, 56, 57, 62, 67, 77, 420, 4426, 9508]
surviving nonsolo ucounts = 3[420, 4426, 9508]
ids = [106, 171, 18]

====================================================================================
NOT paired!

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BARCODE 72.958 = GTGTTAGAGGATGGTC-1

using 46 reads

====================================================================================

graph has 48 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[3^2, 39]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

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BARCODE 72.962 = GTGTTAGAGGCGACAT-1

using 91 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[14, 77]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!
sorting bam, mem = 0.09
running samtools sort -l 8G -o /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_T_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk72-ucc6a7a72e6/files/contig_bam_sorted.bam /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_T_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk72-ucc6a7a72e6/files/contig_bam.bam

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54.454 seconds used processing barcodes, peak mem = 0.26
