[stdout]

starting vdj_asm_asm, mem = 0.01
available mem = 828.60 GB
/scratch/etanis/software/cellranger-6.1.2/lib/bin/cr_vdj martian assembly main /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_T_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk65-ucc6a7a72e6 /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_T_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk65-ucc6a7a72e6/files /scratch/etanis/ST-118/multi/dimitri-multi/journal/SC_MULTI_CS.SC_MULTI_CORE.MULTI_GEM_WELL_PROCESSOR.VDJ_T_GEM_WELL_PROCESSOR.SC_VDJ_CONTIG_ASSEMBLER.ASSEMBLE_VDJ.fork0.chnk65.ucc6a7a72e6
n50_n50_rpu = 2159

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.0 = GGGAATGAGCTTTGGT-1

using 8384 reads

====================================================================================

graph has 7362 edges initially, 244 edges after simplification

total ucounts = 854
nonsolo ucounts = 629[2^85, 3^62, 4^55, 5^35, 6^38, 7^21, 8^33, 9^29, 10^20, 11^15, 12^14, 13^19, 14^17, 15^14, 16^11, 17^16, 18^4, 19^12, 20^7, 21^12, 22^9, 23^8, 24^7, 25^7, 26^11, 27^8, 28^5, 29, 30^4, 31^2, 32^2, 33^2, 34^3, 35^4, 37^3, 38^2, 40, 41^2, 42, 44, 46^2, 47^2, 49, 50, 54, 55, 56, 61, 62^3, 64, 65, 66, 71, 72^2, 75, 79, 80^2, 85, 88, 92, 109]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.2 = GGGAATGAGGACCACA-1

using 263 reads

====================================================================================

graph has 222 edges initially, 6 edges after simplification

total ucounts = 12
nonsolo ucounts = 9[2, 5, 12, 15, 22, 29, 40, 63, 72]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.3 = GGGAATGAGGATGTAT-1

using 231 reads

====================================================================================

graph has 214 edges initially, 6 edges after simplification

total ucounts = 15
nonsolo ucounts = 13[3^2, 4, 5^2, 9, 10, 21, 26, 31^2, 40, 41]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.6 = GGGAATGAGGCCCTTG-1

using 132 reads

====================================================================================

graph has 72 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 5[2^2, 4, 6, 118]
surviving nonsolo ucounts = 1[118]
ids = [3]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.7 = GGGAATGAGGCTAGCA-1

using 77 reads

====================================================================================

graph has 34 edges initially, 4 edges after simplification

total ucounts = 7
nonsolo ucounts = 4[3, 4, 33, 34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.8 = GGGAATGAGGCTCAGA-1

using 86 reads

====================================================================================

graph has 66 edges initially, 4 edges after simplification

total ucounts = 6
nonsolo ucounts = 6[2, 4, 5, 7, 26, 42]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.10 = GGGAATGAGGTGCTTT-1

using 38 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.15 = GGGAATGAGTGGTAAT-1

using 26975 reads

====================================================================================

graph has 11976 edges initially, 197 edges after simplification

total ucounts = 584
nonsolo ucounts = 421[2^61, 3^40, 4^29, 5^22, 6^29, 7^19, 8^12, 9^16, 10^11, 11^11, 12^10, 13^15, 14^15, 15^2, 16^13, 17^9, 18^4, 19^6, 20^10, 21^6, 22^3, 23^6, 24^5, 25^6, 26^3, 27^3, 28^6, 29^6, 31^2, 32^3, 34^2, 35, 38, 39^2, 41^2, 43, 44^2, 45^2, 46, 47, 49, 51^2, 53, 59, 60, 64, 140, 248, 401, 699, 1033, 1034, 1185, 1276, 1400, 1440, 1672, 1710, 2041, 2138, 2540, 3046]
surviving nonsolo ucounts = 15[248, 401, 699, 1033, 1034, 1185, 1276, 1400, 1440, 1672, 1710, 2041, 2138, 2540, 3046]
ids = [376, 167, 192, 112, 183, 317, 39, 19, 390, 582, ...]

====================================================================================

UMI info for barcode GGGAATGAGTGGTAAT-1 contig 1 = AGAGGCCCCA...
umi AAACTTCTGC = 1733 reads: +400 validated
umi AATGCTGCTG = 1174 reads: +400 validated
umi CAGGCGAGGC = 384 reads: +400 validated
umi CATTAACATG = 976 reads: -302X +1 -2X +95 invalidated
umi CCACGTGCTC = 708 reads: +400 validated
umi GCTTTCTCAT = 250 reads: +400 validated
umi TTTTTCGGTC = 1679 reads: +400 validated

UMI info for barcode GGGAATGAGTGGTAAT-1 contig 2 = ATTATTTTTT...
umi AACGGTAAGC = 1368 reads: +403 validated
umi ATCATTTCAA = 1027 reads: +403 validated
umi CTGGGGGTTT = 1164 reads: +403 validated
umi GGCTTCGTCT = 1395 reads: +403 validated

UMI info for barcode GGGAATGAGTGGTAAT-1 contig 3 = AGATCTCTGC...
umi AATGATCACC = 2188 reads: +218 -1XX +190 invalidated
umi CGTAGGGCAA = 2031 reads: +409 validated

GOOD CONTIGS

TIG 1[bases=481]
0-29 ==> 82-111 on |628|TRBV4-3|5'UTR| [len=111] (mis=0)
29-373 ==> 0-344 on |629|TRBV4-3|L-REGION+V-REGION| [len=344] (mis=3)
379-429 ==> 0-50 on |543|TRBJ2-1|J-REGION| [len=50] (mis=3)
429-481 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 6 umis using 375 reads
cdr3 = CASSPGTSFNNEQFF at 356, score = 10 + 8
umis assigned: [3, 39, 167, 183, 192, 376, 582]
of which 7 are surviving nonsolos
reads assigned: 6759
start codons at 29, 83, 122, 128, 151, 179, 221, 286, 387
confident = true

TIG 2[bases=501]
0-56 ==> 0-56 on |461|TRAV12-3|5'UTR| [len=56] (mis=0)
56-399 ==> 0-343 on |462|TRAV12-3|L-REGION+V-REGION| [len=343] (mis=3)
396-459 ==> 0-63 on |435|TRAJ48|J-REGION| [len=63] (mis=4)
459-501 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 4 umis using 206 reads
cdr3 = CAMGTYFGNEKLTF at 386, score = 9 + 10
umis assigned: [19, 112, 317, 390]
of which 4 are surviving nonsolos
reads assigned: 4853
start codons at 56, 59, 221, 263, 294, 392, 411
confident = true

TIG 3[bases=470]
0-19 ==> 17-36 on |455|TRAV10|5'UTR| [len=36] (mis=0)
19-362 ==> 0-343 on |456|TRAV10|L-REGION+V-REGION| [len=343] (mis=2)
362-428 ==> 0-66 on |413|TRAJ28|J-REGION| [len=66] (mis=0)
428-470 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 2 umis using 253 reads
cdr3 = CVVSSYSGAGSYQLTF at 349, score = 6 + 10
umis assigned: [35, 276]
of which 2 are surviving nonsolos
reads assigned: 4073
start codons at 19, 77, 147, 229, 281
confident = true

REJECT CONTIGS

TIG 1[bases=471]
0-24 ==> 23-47 on |692|TRBV9|5'UTR| [len=47] (mis=0)
24-367 ==> 0-343 on |693|TRBV9|L-REGION+V-REGION| [len=343] (mis=0)
369-419 ==> 0-50 on |543|TRBJ2-1|J-REGION| [len=50] (mis=4)
419-471 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
umis assigned: [97, 179]
of which 2 are surviving nonsolos
reads assigned: 5508
start codons at 24, 146, 232, 377
confident = false
did not find CDR3
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.19 = GGGAATGAGTTGAGAT-1

using 34 reads

====================================================================================

graph has 27 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.22 = GGGAATGCAAATACAG-1

using 1493 reads

====================================================================================

graph has 732 edges initially, 22 edges after simplification

total ucounts = 56
nonsolo ucounts = 49[2^7, 3^5, 4^2, 5^2, 6, 7, 8, 12^2, 13, 14, 15, 18, 20^2, 21, 30, 31^2, 37, 41^2, 43, 46, 47, 48^2, 52^2, 56, 62, 73, 98, 102, 104, 110, 121]
surviving nonsolo ucounts = 1[47]
ids = [40]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.23 = GGGAATGCAACAACCT-1

using 36 reads

====================================================================================

graph has 76 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.30 = GGGAATGCAATGGACG-1

using 69 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[69]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.31 = GGGAATGCACATCCAA-1

using 87 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[87]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.33 = GGGAATGCACCAGTTA-1

using 25 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.34 = GGGAATGCACCGTTGG-1

using 240 reads

====================================================================================

graph has 216 edges initially, 10 edges after simplification

total ucounts = 20
nonsolo ucounts = 17[2, 3^3, 4^3, 5, 7^2, 9^2, 18, 21, 40, 42, 56]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.37 = GGGAATGCACTGTTAG-1

using 41 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[41]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.45 = GGGAATGCAGCTCCGA-1

using 31 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.53 = GGGAATGCATCACCCT-1

using 19 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.61 = GGGAATGCATTAGGCT-1

using 30 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.69 = GGGAATGGTACTCAAC-1

using 53 reads

====================================================================================

graph has 62 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[53]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.70 = GGGAATGGTACTTCTT-1

using 40 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[40]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.72 = GGGAATGGTAGCCTAT-1

using 73 reads

====================================================================================

graph has 74 edges initially, 4 edges after simplification

total ucounts = 7
nonsolo ucounts = 4[5, 6, 22, 37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.74 = GGGAATGGTATATCCG-1

using 54 reads

====================================================================================

graph has 66 edges initially, 4 edges after simplification

total ucounts = 7
nonsolo ucounts = 6[3^4, 17, 24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.81 = GGGAATGGTCCCTTGT-1

using 32875 reads

====================================================================================

graph has 11166 edges initially, 132 edges after simplification

total ucounts = 523
nonsolo ucounts = 376[2^62, 3^33, 4^40, 5^23, 6^25, 7^22, 8^20, 9^12, 10^8, 11^9, 12^6, 13^9, 14^4, 15^12, 16^9, 17^6, 18^2, 19^3, 20^5, 21^5, 22, 23^10, 24^3, 25^8, 27^4, 28, 29, 30, 31, 32, 39, 44, 45, 47, 52^2, 55, 57^2, 59, 64, 65, 71, 82, 97, 721, 829, 999, 1153^2, 1496, 1554, 1619, 1820, 1980, 2061^2, 2235, 3256, 5905]
surviving nonsolo ucounts = 16[64, 721, 829, 999, 1153^2, 1496, 1554, 1619, 1820, 1980, 2061^2, 2235, 3256, 5905]
ids = [493, 321, 339, 9, 213, 501, 47, 251, 490, 301, ...]

====================================================================================

UMI info for barcode GGGAATGGTCCCTTGT-1 contig 1 = GGGGAGTTTC...
umi AACGGGCACG = 1007 reads: +397 validated
umi GGGTTAGATT = 709 reads: +324 -56 +17 non-validated
umi GTCTAAGATC = 827 reads: +397 validated
umi TTCTTGTGCT = 65 reads: +316 -64 +5 -1 +2 -1 +4 -4 non-validated

UMI info for barcode GGGAATGGTCCCTTGT-1 contig 2 = CCTTTTTCTC...
umi ACGTCTTTCT = 1470 reads: +397 validated
umi CTTTTTTCGA = 1582 reads: +48 -1XX +348 invalidated
umi GCCTGTCCAA = 2251 reads: +397 validated
umi GCTAAACCCC = 1821 reads: +397 validated
umi GGCCTCTTAG = 2071 reads: +397 validated
umi TATGAGCTCC = 2094 reads: +44 -2XX +2 -1XX +348 invalidated
umi TCACTTCTCG = 2007 reads: +397 validated
umi TTCTCTTTCA = 1634 reads: +45 -1XX +2 -1XX +348 invalidated

GOOD CONTIGS

TIG 1[bases=677]
238-579 ==> 0-341 on |522|TRAV8-3|L-REGION+V-REGION| [len=341] (mis=2)
577-635 ==> 0-58 on |420|TRAJ34|J-REGION| [len=58] (mis=4)
635-677 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 2 umis using 26 reads
cdr3 = CAVAAWNTDKLIF at 565, score = 7 + 9
umis assigned: [9, 321, 339, 493]
of which 4 are surviving nonsolos
reads assigned: 2561
start codons at 238, 360, 374, 401, 548
confident = true

TIG 2[bases=508]
0-59 ==> 0-59 on |576|TRBV14|5'UTR| [len=59] (mis=0)
59-406 ==> 0-347 on |577|TRBV14|L-REGION+V-REGION| [len=347] (mis=0)
408-456 ==> 0-48 on |548|TRBJ2-5|J-REGION| [len=48] (mis=5)
456-508 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 8 umis using 1017 reads
cdr3 = CASSQDIAETQYF at 389, score = 9 + 7
umis assigned: [47, 251, 294, 301, 318, 383, 394, 490]
of which 8 are surviving nonsolos
reads assigned: 14620
start codons at 10, 36, 59, 181, 201, 230, 282, 293
confident = true

REJECT CONTIGS

TIG 1[bases=456]
0-47 ==> 0-47 on |692|TRBV9|5'UTR| [len=47] (mis=0)
47-390 ==> 0-343 on |693|TRBV9|L-REGION+V-REGION| [len=343] (mis=0)
404-456 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
umis assigned: [213, 282, 447, 501]
of which 4 are surviving nonsolos
reads assigned: 10518
start codons at 3, 47, 169, 255
confident = false
did not find CDR3
now this is a cell
paired!

GTGCATTGGAGTGATGCTGCTGAGTACTTCTGTGCTGTGGCCGCGTGGAACACCGACAAGCTCATCTTTGGGACTGGGACCAGATTACAAGTCTTTCCAA <==> CCTGCAGAACTGGAGGATTCTGGAGTTTATTTCTGTGCCAGCAGCCAAGACATTGCGGAGACCCAGTACTTCGGGCCAGGCACGCGGCTCCTGGTGCTCG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.82 = GGGAATGGTCCGACGT-1

using 140 reads

====================================================================================

graph has 233 edges initially, 4 edges after simplification

total ucounts = 9
nonsolo ucounts = 6[3, 4, 7, 11, 32, 80]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.89 = GGGAATGGTCTCTCGT-1

using 83 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[83]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.91 = GGGAATGGTCTTCAAG-1

using 65 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 5[5, 6, 10, 17, 26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.93 = GGGAATGGTGATAAAC-1

using 60 reads

====================================================================================

graph has 64 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[2, 58]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.94 = GGGAATGGTGCACGAA-1

using 192 reads

====================================================================================

graph has 130 edges initially, 8 edges after simplification

total ucounts = 11
nonsolo ucounts = 8[2, 6, 7^2, 8, 12, 71, 76]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.97 = GGGAATGGTGCCTTGG-1

using 34 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.99 = GGGAATGGTGGTCCGT-1

using 27 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.101 = GGGAATGGTGTGGTTT-1

using 19 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.114 = GGGAATGTCACAGTAC-1

using 39 reads

====================================================================================

graph has 30 edges initially, 4 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[15, 23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.119 = GGGAATGTCAGTGCAT-1

using 14324 reads

====================================================================================

graph has 11830 edges initially, 380 edges after simplification

total ucounts = 1314
nonsolo ucounts = 992[2^143, 3^85, 4^74, 5^69, 6^54, 7^51, 8^32, 9^33, 10^38, 11^29, 12^42, 13^26, 14^32, 15^26, 16^28, 17^11, 18^15, 19^12, 20^10, 21^12, 22^12, 23^14, 24^14, 25^6, 26^5, 27^8, 28^7, 29^7, 30^4, 31^10, 32^3, 33^5, 34^6, 35^2, 36^3, 37, 38^4, 39^2, 40, 41, 42^4, 43^2, 44^5, 45^2, 46^2, 47, 48, 50^4, 51, 52^2, 53^2, 54, 55^2, 56, 58, 62, 63^2, 64, 66^2, 69^3, 75^2, 77, 80, 81, 89^2, 97^2, 99, 101, 103, 109, 165, 966]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.122 = GGGAATGTCATCACCC-1

using 21 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.123 = GGGAATGTCATGGTCA-1

using 36 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 1[33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.124 = GGGAATGTCCAAATGC-1

using 41 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[7, 10, 22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.132 = GGGAATGTCCTCATTA-1

using 45 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[45]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.134 = GGGAATGTCCTTTACA-1

using 148 reads

====================================================================================

graph has 286 edges initially, 14 edges after simplification

total ucounts = 11
nonsolo ucounts = 11[2^3, 3^2, 4^2, 5, 12, 25, 86]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.141 = GGGAATGTCGTACCGG-1

using 63 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[7, 55]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.144 = GGGAATGTCTAACTCT-1

using 66 reads

====================================================================================

graph has 64 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 8[2^2, 3^3, 4, 5, 44]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.146 = GGGAATGTCTCGAGTA-1

using 24 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.147 = GGGAATGTCTGAAAGA-1

using 54168 reads

====================================================================================

graph has 17198 edges initially, 228 edges after simplification

total ucounts = 581
nonsolo ucounts = 430[2^44, 3^33, 4^44, 5^27, 6^14, 7^14, 8^19, 9^14, 10^16, 11^12, 12^7, 13^6, 14^14, 15^10, 16^9, 17^12, 18^4, 19^10, 20^5, 21^3, 22^11, 23^3, 24^4, 26^5, 27^7, 28^7, 29^6, 30^2, 31^3, 32^3, 33^3, 34, 35, 36^3, 37, 38^3, 39^3, 40^3, 41^2, 42, 44, 48^2, 49, 50^2, 51, 52, 54, 58, 59, 66, 67, 68, 84, 86, 88, 98, 162, 432, 464, 694, 815, 891, 1081, 1558, 1650, 2014, 2142, 2207, 2262, 2362, 2431, 2485, 2500, 2537, 2541, 2582, 2599, 3419, 8405]
surviving nonsolo ucounts = 22[162, 464, 694, 815, 891, 1081, 1558, 1650, 2014, 2142, 2207, 2262, 2362, 2431, 2485, 2500, 2537, 2541, 2582, 2599, 3419, 8405]
ids = [137, 18, 331, 266, 186, 162, 204, 437, 574, 346, ...]

====================================================================================

UMI info for barcode GGGAATGTCTGAAAGA-1 contig 1 = AGACACAGTG...
umi ACCCTTACCC = 465 reads: +395 -1 +7 non-validated
umi ACCGGTTTCC = 2453 reads: +403 validated
umi AGTAACCTCC = 2379 reads: +403 validated
umi CATGCGCTTA = 2263 reads: +403 validated
umi CATTACGCAT = 3427 reads: +403 validated
umi CCAAGACAAT = 1084 reads: +403 validated
umi CCTCTTTCCT = 1549 reads: +403 validated
umi CGTAATCAAC = 2251 reads: +310 -5XX +88 invalidated
umi CTTATCACAA = 2573 reads: +403 validated
umi GATGGTTCCA = 2607 reads: +403 validated
umi GCACTACCGT = 2142 reads: +403 validated
umi GCTAGCCCGT = 7192 reads: -334X +5 -1XX +2 -1XX +1 -2XX +1 -5XX +1 -7XX +43 invalidated
umi TCGTATAGGC = 2498 reads: +403 validated
umi TCTGAGTCGC = 2488 reads: +403 validated
umi TGTAAAAGGG = 2549 reads: +403 validated
umi TGTTACACCG = 2559 reads: +403 validated
umi TTTCTATGTT = 2020 reads: +403 validated

UMI info for barcode GGGAATGTCTGAAAGA-1 contig 2 = GGGGGTAGTT...
umi CATCTTACGG = 130 reads: +374 -4 +22 non-validated
umi CCCGTACACC = 774 reads: +356 -1 +3 -23 +17 non-validated
umi CTATATCGTG = 748 reads: +400 validated
umi GATATAGGTA = 703 reads: +400 validated
umi TAGTTCTTTA = 1670 reads: +400 validated

GOOD CONTIGS

TIG 1[bases=484]
0-29 ==> 280-309 on |630|TRBV5-1|5'UTR| [len=309] (mis=0)
29-372 ==> 0-343 on |631|TRBV5-1|L-REGION+V-REGION| [len=343] (mis=4)
371-387 ==> 0-16 on |730|TRBD2|D-REGION| [len=16] (mis=2)
389-432 ==> 7-50 on |543|TRBJ2-1|J-REGION| [len=50] (mis=0)
432-484 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 14 umis using 1986 reads
cdr3 = CASSALTSGRVNEQFF at 356, score = 9 + 8
umis assigned: [18, 22, 47, 140, 146, 162, 204, 234, 304, 338] and 7 others
of which 17 are surviving nonsolos
reads assigned: 41619
start codons at 29, 311, 315, 390
confident = true

TIG 2[bases=581]
139-480 ==> 0-341 on |522|TRAV8-3|L-REGION+V-REGION| [len=341] (mis=0)
477-539 ==> 0-62 on |446|TRAJ6|J-REGION| [len=62] (mis=0)
539-581 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 3 umis using 92 reads
cdr3 = CAVGASGGSYIPTF at 466, score = 7 + 7
umis assigned: [137, 186, 266, 331, 437]
of which 5 are surviving nonsolos
reads assigned: 3942
start codons at 139, 261, 275, 302, 449
confident = true
now this is a cell
paired!

CATTGGAGTGATGCTGCTGAGTACTTCTGTGCTGTGGGTGCATCAGGAGGAAGCTACATACCTACATTTGGAAGAGGAACCAGCCTTATTGTTCATCCGT <==> CTGGGGGACTCGGCCCTTTATCTTTGCGCCAGCAGCGCCCTGACTAGCGGGAGGGTGAATGAGCAGTTCTTCGGGCCAGGGACACGGCTCACCGTGCTAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.149 = GGGAATGTCTGTCAAG-1

using 90 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[90]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.151 = GGGAATGTCTTAGCCC-1

using 38269 reads

====================================================================================

graph has 10887 edges initially, 114 edges after simplification

total ucounts = 472
nonsolo ucounts = 339[2^56, 3^35, 4^24, 5^33, 6^25, 7^24, 8^15, 9^13, 10^10, 11^11, 12^8, 13^8, 14^5, 15^3, 16^2, 17^5, 18^4, 19^8, 20^2, 21^4, 22^2, 23^5, 24^4, 25, 26, 27^4, 28^3, 30, 32, 33, 35, 47, 50, 54, 55^2, 64, 163, 596, 919, 969, 1014, 1399, 1866, 1870, 1939, 2032, 2113, 3016, 7775, 9478]
surviving nonsolo ucounts = 13[596, 919, 969, 1014, 1399, 1866, 1870, 1939, 2032, 2113, 3016, 7775, 9478]
ids = [93, 448, 73, 299, 78, 75, 90, 255, 356, 133, ...]

====================================================================================

UMI info for barcode GGGAATGTCTTAGCCC-1 contig 1 = AGAGGCCCCA...
umi AGACCAACAA = 8038 reads: -375 +2 -1XX +1 -3XX +4 -1XX +2 -1XX +2 -2XX +1 -1XX +1 invalidated
umi AGAGGGGGGG = 1874 reads: +397 validated
umi AGATCATTAG = 1287 reads: -375 +2 -1XX +1 -3XX +7 -1XX +2 -2XX +3 invalidated
umi AGGCGCACAC = 1880 reads: +397 validated
umi CACTCACGGC = 2116 reads: +397 validated
umi CTTGTGTGAT = 6508 reads: -376 +1 -1XX +1 -3XX +4 -1XX +2 -1XX +2 -2XX +1 -1XX +1 invalidated
umi TATCTATAGC = 2038 reads: +397 validated
umi TGATCGTGTG = 3029 reads: +397 validated

UMI info for barcode GGGAATGTCTTAGCCC-1 contig 2 = AGATTTGCAG...
umi AGAGAGTCGG = 961 reads: +430 validated
umi AGGTCTATTT = 108 reads: -399 +2 -2XX +1 -1XX +7 -3XX +1 -2XX +1 -1XX +3 -3XX +4 invalidated
umi GATACAACTG = 1976 reads: +430 validated
umi GGTAATCCCT = 979 reads: +430 validated
umi TTCGAGTATA = 881 reads: +430 validated

GOOD CONTIGS

TIG 1[bases=478]
0-29 ==> 82-111 on |626|TRBV4-2|5'UTR| [len=111] (mis=0)
29-373 ==> 0-344 on |627|TRBV4-2|L-REGION+V-REGION| [len=344] (mis=3)
376-426 ==> 0-50 on |538|TRBJ1-3|J-REGION| [len=50] (mis=0)
426-478 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 5 umis using 871 reads
cdr3 = CASSPILSGNTIYF at 356, score = 10 + 7
umis assigned: [69, 75, 78, 90, 133, 231, 356, 407]
of which 8 are surviving nonsolos
reads assigned: 26549
start codons at 29, 83, 122, 128, 151, 179, 221, 286
confident = true

TIG 2[bases=526]
0-54 ==> 57-111 on |494|TRAV29/DV5|5'UTR| [len=111] (mis=1)
54-412 ==> 0-358 on |495|TRAV29/DV5|L-REGION+V-REGION| [len=358] (mis=2)
418-484 ==> 0-66 on |413|TRAJ28|J-REGION| [len=66] (mis=2)
484-526 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 4 umis using 225 reads
cdr3 = CAASPFTNSGAGSYQLTF at 399, score = 9 + 10
umis assigned: [73, 93, 255, 299, 448]
of which 5 are surviving nonsolos
reads assigned: 4786
start codons at 54, 60, 127, 130, 216, 233, 301, 307
confident = true
now this is a cell
paired!

TCTGCAGTGTACTTCTGTGCAGCAAGCCCCTTTACCAACTCTGGGGCTGGGAGTTACCAACTCACTTTCGGGAAGGGGACCAAACTCTCGGTCATACCAA <==> CTGCAGCCAGAAGACTCGGCCCTGTATCTCTGTGCCAGCAGCCCAATCCTCTCTGGAAACACCATATATTTTGGAGAGGGAAGTTGGCTCACTGTTGTAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.153 = GGGAATGTCTTCGAGA-1

using 113 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[112]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.154 = GGGAATGTCTTCGGTC-1

using 66 reads

====================================================================================

graph has 159 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[66]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.155 = GGGAATGTCTTGGGTA-1

using 96 reads

====================================================================================

graph has 96 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 6[4, 9, 11, 16, 17, 38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.156 = GGGAATGTCTTGTACT-1

using 30 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 1[27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.158 = GGGACCTAGAAACCTA-1

using 259 reads

====================================================================================

graph has 194 edges initially, 10 edges after simplification

total ucounts = 17
nonsolo ucounts = 15[2^3, 6, 7, 9^2, 10, 14^2, 15, 21, 41, 46, 59]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.159 = GGGACCTAGAAAGTGG-1

using 77 reads

====================================================================================

graph has 54 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[77]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.160 = GGGACCTAGAATCTCC-1

using 16 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[16]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.168 = GGGACCTAGAGATGAG-1

using 34 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.171 = GGGACCTAGATAGGAG-1

using 47 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 5[2, 4^2, 5, 31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.172 = GGGACCTAGATCGATA-1

using 30 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 1[27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.173 = GGGACCTAGATCGGGT-1

using 34 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.174 = GGGACCTAGCAATATG-1

using 41 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 1[38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.176 = GGGACCTAGCCAGTTT-1

using 110 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[110]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.177 = GGGACCTAGCCCAACC-1

using 17 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[17]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.182 = GGGACCTAGCTGCAAG-1

using 49 reads

====================================================================================

graph has 56 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[2, 3, 9, 33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.192 = GGGACCTAGTACGATA-1

using 72 reads

====================================================================================

graph has 84 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[72]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.196 = GGGACCTAGTCTTGCA-1

using 1717 reads

====================================================================================

graph has 486 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[1717]
surviving nonsolo ucounts = 1[1717]
ids = [0]

====================================================================================

UMI info for barcode GGGACCTAGTCTTGCA-1 contig 1 = ACAGTGATCC...
umi CAAGTGGGGT = 1712 reads: +403 validated

GOOD CONTIGS

TIG 1[bases=502]
0-47 ==> 0-47 on |674|TRBV7-2|5'UTR| [len=47] (mis=0)
47-394 ==> 0-347 on |675|TRBV7-2|L-REGION+V-REGION| [len=347] (mis=4)
402-450 ==> 0-48 on |548|TRBJ2-5|J-REGION| [len=48] (mis=2)
450-502 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 1 umis using 87 reads
cdr3 = CASSPTSGRQETQYF at 377, score = 8 + 7
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 1687
start codons at 47, 156
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.198 = GGGACCTAGTGCAAGC-1

using 49 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[2^2, 6, 37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.201 = GGGACCTAGTTTCCTT-1

using 16 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[16]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.205 = GGGACCTCAACACCTA-1

using 35 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.207 = GGGACCTCAACGATCT-1

using 1375 reads

====================================================================================

graph has 983 edges initially, 20 edges after simplification

total ucounts = 228
nonsolo ucounts = 151[2^28, 3^26, 4^19, 5^19, 6^8, 7^8, 8^7, 9^6, 10^5, 11^3, 12, 14^4, 16^2, 18, 21, 22, 25, 30, 32, 36, 38, 39, 41, 46, 52, 53, 68, 75]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.210 = GGGACCTCAATCTACG-1

using 133 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[133]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.215 = GGGACCTCACACCGCA-1

using 82 reads

====================================================================================

graph has 72 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[82]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.216 = GGGACCTCACCAGATT-1

using 206 reads

====================================================================================

graph has 105 edges initially, 8 edges after simplification

total ucounts = 13
nonsolo ucounts = 11[2^2, 3, 4, 10, 15, 16^2, 34, 46, 56]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.219 = GGGACCTCACGAAAGC-1

using 37 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.223 = GGGACCTCACTTGGAT-1

using 59 reads

====================================================================================

graph has 48 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[2, 3, 7, 45]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.232 = GGGACCTCAGCGTAAG-1

using 76 reads

====================================================================================

graph has 48 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 4[2^3, 67]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.233 = GGGACCTCAGCTCCGA-1

using 59 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[58]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.237 = GGGACCTCAGTAGAGC-1

using 23 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 5[2^2, 3, 5, 10]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.242 = GGGACCTCATCACGTA-1

using 99 reads

====================================================================================

graph has 62 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[11, 88]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.243 = GGGACCTCATCAGTCA-1

using 58 reads

====================================================================================

graph has 74 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 4[6, 9, 18, 22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.249 = GGGACCTCATGCCACG-1

using 740 reads

====================================================================================

graph has 250 edges initially, 2 edges after simplification

total ucounts = 51
nonsolo ucounts = 42[2^2, 3^7, 4^5, 5^5, 6^4, 7, 8, 11, 12, 13, 14, 16, 24, 31^2, 38, 41, 45, 47, 49, 51, 52, 73, 74]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.253 = GGGACCTCATGTCGAT-1

using 30 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.265 = GGGACCTGTACCAGTT-1

using 31 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.272 = GGGACCTGTAGTGAAT-1

using 45 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[45]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.275 = GGGACCTGTATTCGTG-1

using 75 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[75]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.277 = GGGACCTGTCAACTGT-1

using 102 reads

====================================================================================

graph has 94 edges initially, 4 edges after simplification

total ucounts = 7
nonsolo ucounts = 5[2, 3, 16, 37, 42]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.278 = GGGACCTGTCACACGC-1

using 5072 reads

====================================================================================

graph has 3690 edges initially, 98 edges after simplification

total ucounts = 537
nonsolo ucounts = 370[2^74, 3^57, 4^30, 5^34, 6^22, 7^19, 8^14, 9^12, 10^12, 11^10, 12^12, 13^2, 14^9, 15^4, 16^3, 17^6, 18^4, 19^4, 20^3, 21^3, 23^3, 24, 25^2, 26^4, 27^2, 28^3, 31, 33, 34^2, 35, 37, 39^2, 40, 41, 42, 46, 50, 54^2, 55, 56, 67, 71, 105, 1456]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.292 = GGGACCTGTCTCTCTG-1

using 17230 reads

====================================================================================

graph has 9418 edges initially, 168 edges after simplification

total ucounts = 1203
nonsolo ucounts = 854[2^162, 3^110, 4^71, 5^82, 6^49, 7^58, 8^33, 9^36, 10^21, 11^29, 12^20, 13^23, 14^18, 15^12, 16^17, 17^12, 18^12, 19^9, 20^3, 21^7, 22^3, 23^2, 24^6, 25^8, 26^2, 27^5, 28^4, 29, 30^6, 31^2, 32, 36, 37, 38, 39, 40, 41, 43^2, 44^2, 45, 46^3, 47, 48, 49, 50, 53, 55, 58, 59^2, 65, 69, 70, 71, 78, 1115, 8083]
surviving nonsolo ucounts = 2[1115, 8083]
ids = [220, 485]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.294 = GGGACCTGTCTCTTTA-1

using 55 reads

====================================================================================

graph has 58 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 4[5^3, 37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.303 = GGGACCTGTGTTTGTG-1

using 59 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 1[57]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.313 = GGGACCTGTTCTGTTT-1

using 28 reads

====================================================================================

graph has 6 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.319 = GGGACCTGTTTAGCTG-1

using 56 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 5[3^2, 7, 10, 31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.324 = GGGACCTTCAATCACG-1

using 25 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.327 = GGGACCTTCACCTCGT-1

using 48 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[48]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.330 = GGGACCTTCACTGGGC-1

using 75 reads

====================================================================================

graph has 84 edges initially, 2 edges after simplification

total ucounts = 18
nonsolo ucounts = 14[2^3, 3, 4^3, 5^2, 6^2, 8, 9, 11]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.331 = GGGACCTTCACTTACT-1

using 72 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[11, 61]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.333 = GGGACCTTCAGCACAT-1

using 86 reads

====================================================================================

graph has 86 edges initially, 2 edges after simplification

total ucounts = 14
nonsolo ucounts = 7[2, 4, 6^2, 9, 11, 41]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.334 = GGGACCTTCAGCCTAA-1

using 236 reads

====================================================================================

graph has 158 edges initially, 14 edges after simplification

total ucounts = 19
nonsolo ucounts = 11[2^3, 4, 5, 16, 23^2, 24, 30, 97]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.340 = GGGACCTTCATCATTC-1

using 321 reads

====================================================================================

graph has 192 edges initially, 8 edges after simplification

total ucounts = 17
nonsolo ucounts = 14[2^3, 4, 5, 6, 11^2, 16^2, 33, 34, 75, 101]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.344 = GGGACCTTCATTATCC-1

using 56 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[56]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.345 = GGGACCTTCATTGCCC-1

using 34 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[4, 8, 20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.348 = GGGACCTTCCAGGGCT-1

using 48 reads

====================================================================================

graph has 52 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[10, 38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.349 = GGGACCTTCCAGTAGT-1

using 34 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[3, 30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.350 = GGGACCTTCCATGAAC-1

using 29 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.351 = GGGACCTTCCCAACGG-1

using 38 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 4[3, 5, 7, 23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.352 = GGGACCTTCCCAAGTA-1

using 38 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 7[2^2, 3, 4, 5, 7, 13]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.355 = GGGACCTTCCCTGACT-1

using 57 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[57]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.356 = GGGACCTTCCCTTGCA-1

using 48 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[48]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.359 = GGGACCTTCCGTACAA-1

using 50 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[3, 7, 39]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.369 = GGGACCTTCGGCTACG-1

using 55 reads

====================================================================================

graph has 82 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 5[2, 3, 9, 11, 26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.371 = GGGACCTTCGGTGTTA-1

using 15380 reads

====================================================================================

graph has 13129 edges initially, 422 edges after simplification

total ucounts = 1648
nonsolo ucounts = 1214[2^191, 3^116, 4^86, 5^86, 6^58, 7^55, 8^60, 9^55, 10^43, 11^35, 12^32, 13^26, 14^32, 15^32, 16^20, 17^24, 18^16, 19^21, 20^22, 21^16, 22^14, 23^18, 24^7, 25^10, 26^14, 27^8, 28^6, 29^9, 30^9, 31^8, 32^3, 33^6, 34^6, 35^3, 36^2, 37^4, 38^2, 39^6, 41^5, 42^5, 43^2, 44, 45^2, 46^3, 47, 48^2, 49, 51, 52^2, 55, 56^2, 58, 60, 61, 62, 63^3, 65, 66^2, 68^2, 70^2, 72, 79, 82, 86, 88, 106, 108, 109, 115, 120, 123]
surviving nonsolo ucounts = 1[20]
ids = [1210]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.372 = GGGACCTTCGGTTAAC-1

using 41 reads

====================================================================================

graph has 48 edges initially, 2 edges after simplification

total ucounts = 13
nonsolo ucounts = 3[4, 11, 16]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.373 = GGGACCTTCGTCACGG-1

using 578 reads

====================================================================================

graph has 396 edges initially, 10 edges after simplification

total ucounts = 34
nonsolo ucounts = 27[2^4, 3^3, 7, 8^2, 10^2, 15, 16^2, 17, 18, 25, 31, 34, 42^2, 44, 46, 52, 54, 59]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.378 = GGGACCTTCTGATTCT-1

using 119 reads

====================================================================================

graph has 116 edges initially, 4 edges after simplification

total ucounts = 19
nonsolo ucounts = 10[2, 3, 4^2, 5, 6^2, 7, 24, 49]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.379 = GGGACCTTCTGCAGTA-1

using 7201 reads

====================================================================================

graph has 6687 edges initially, 200 edges after simplification

total ucounts = 882
nonsolo ucounts = 642[2^102, 3^74, 4^61, 5^44, 6^39, 7^39, 8^30, 9^26, 10^25, 11^25, 12^18, 13^14, 14^13, 15^13, 16^14, 17^11, 18^9, 19^8, 20^6, 21^4, 22^5, 23^5, 24^5, 25^4, 26^2, 27^2, 28^2, 29^5, 30^2, 31^3, 34, 35, 36^2, 38, 39^3, 41^2, 42^2, 43^2, 44, 49^2, 51, 52^2, 55, 58, 61, 62, 64, 66, 69, 75, 83, 88, 105, 325]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.384 = GGGACCTTCTGGGCCA-1

using 34 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.385 = GGGACCTTCTGGTGTA-1

using 79 reads

====================================================================================

graph has 88 edges initially, 2 edges after simplification

total ucounts = 13
nonsolo ucounts = 10[2^3, 4, 5, 6^2, 11, 15, 23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.389 = GGGACCTTCTTCATGT-1

using 24 reads

====================================================================================

graph has 4 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.391 = GGGACCTTCTTGAGAC-1

using 124 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[124]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.396 = GGGAGATAGAACAATC-1

using 219 reads

====================================================================================

graph has 176 edges initially, 6 edges after simplification

total ucounts = 9
nonsolo ucounts = 9[2^2, 5^2, 7^2, 43, 48, 100]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.405 = GGGAGATAGATCCCAT-1

using 50 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 2[4, 40]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.406 = GGGAGATAGCAAATCA-1

using 242 reads

====================================================================================

graph has 200 edges initially, 6 edges after simplification

total ucounts = 18
nonsolo ucounts = 14[3^2, 4^3, 5, 7, 9, 15, 17, 18, 25, 34, 90]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.408 = GGGAGATAGCATCATC-1

using 40 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[40]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.410 = GGGAGATAGCCAGGAT-1

using 83 reads

====================================================================================

graph has 84 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 2[24, 55]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.413 = GGGAGATAGCTAACAA-1

using 155 reads

====================================================================================

graph has 138 edges initially, 8 edges after simplification

total ucounts = 13
nonsolo ucounts = 9[2, 5, 6, 8, 12, 15, 17, 40, 46]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.414 = GGGAGATAGGAATCGC-1

using 70 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[70]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.417 = GGGAGATAGGAGTCTG-1

using 154 reads

====================================================================================

graph has 70 edges initially, 4 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[14, 139]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.420 = GGGAGATAGGGAAACA-1

using 99 reads

====================================================================================

graph has 54 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[99]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.421 = GGGAGATAGGGCACTA-1

using 93 reads

====================================================================================

graph has 82 edges initially, 4 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[3, 6, 35, 47]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.422 = GGGAGATAGGGCTCTC-1

using 522 reads

====================================================================================

graph has 422 edges initially, 16 edges after simplification

total ucounts = 20
nonsolo ucounts = 19[2, 4, 5, 6, 7, 8, 10, 15, 18^3, 19, 25, 27, 37, 44, 66, 95, 97]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.427 = GGGAGATAGGTGCTTT-1

using 22 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.428 = GGGAGATAGTAACCCT-1

using 44 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[17, 27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.432 = GGGAGATAGTCCCACG-1

using 31 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.436 = GGGAGATAGTGCCAGA-1

using 70 reads

====================================================================================

graph has 96 edges initially, 2 edges after simplification

total ucounts = 13
nonsolo ucounts = 9[2^2, 3^3, 5, 8, 12, 28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.437 = GGGAGATAGTGGGATC-1

using 110 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[110]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.449 = GGGAGATCAATGGAGC-1

using 2451 reads

====================================================================================

graph has 2113 edges initially, 76 edges after simplification

total ucounts = 233
nonsolo ucounts = 149[2^27, 3^21, 4^12, 5^16, 6^4, 7^7, 8^3, 9^2, 10^4, 11^2, 12^2, 13, 14^4, 15^2, 16^2, 17^2, 18, 19, 20, 21, 22^2, 23^2, 24^2, 25, 26, 29, 30^2, 32^2, 33, 34, 37, 38, 39, 42, 43, 45, 52, 55^2, 57, 73, 74, 75, 80, 88, 94, 105, 107, 110]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.451 = GGGAGATCACAAGACG-1

using 43 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[42]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.458 = GGGAGATCACGGACAA-1

using 32 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[2, 29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.459 = GGGAGATCACGGTGTC-1

using 43 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[43]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.465 = GGGAGATCAGCAGTTT-1

using 94 reads

====================================================================================

graph has 64 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[94]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.467 = GGGAGATCAGCGTAAG-1

using 42 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[42]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.470 = GGGAGATCAGGAATCG-1

using 81 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[81]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.474 = GGGAGATCAGTCACTA-1

using 62 reads

====================================================================================

graph has 66 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 8[2^2, 4, 5, 7, 8, 9, 23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.486 = GGGAGATCATTACGAC-1

using 39 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[39]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.487 = GGGAGATCATTTCAGG-1

using 93 reads

====================================================================================

graph has 80 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 5[8, 10, 12, 31, 32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.492 = GGGAGATGTACAGACG-1

using 127 reads

====================================================================================

graph has 176 edges initially, 6 edges after simplification

total ucounts = 9
nonsolo ucounts = 7[2^2, 3, 6, 8, 42, 62]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.493 = GGGAGATGTACCGTAT-1

using 55 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[55]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.494 = GGGAGATGTACGAAAT-1

using 543 reads

====================================================================================

graph has 350 edges initially, 12 edges after simplification

total ucounts = 91
nonsolo ucounts = 16[2^3, 4, 6, 7, 8, 10^2, 12, 14, 32, 66, 79, 100, 114]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.499 = GGGAGATGTATAGGGC-1

using 32 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.507 = GGGAGATGTCTAGGTT-1

using 89 reads

====================================================================================

graph has 108 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 4[14, 18, 19, 37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.512 = GGGAGATGTGTATGGG-1

using 9 reads

====================================================================================

graph has 4 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[9]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.513 = GGGAGATGTGTTTGGT-1

using 80 reads

====================================================================================

graph has 52 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[80]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.519 = GGGAGATGTTGATTGC-1

using 49 reads

====================================================================================

graph has 52 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 4[2^2, 21^2]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.535 = GGGAGATTCCAATGGT-1

using 89 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[5, 16, 67]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.536 = GGGAGATTCCACGAAT-1

using 19 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.541 = GGGAGATTCCGTTGTC-1

using 35 reads

====================================================================================

graph has 52 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.543 = GGGAGATTCCTTGGTC-1

using 19 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.547 = GGGAGATTCGGAATCT-1

using 54 reads

====================================================================================

graph has 62 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 5[3^2, 4, 19, 24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.549 = GGGAGATTCGGCTTGG-1

using 17 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[17]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.552 = GGGAGATTCTAACTTC-1

using 67 reads

====================================================================================

graph has 88 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 2[3, 60]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.554 = GGGAGATTCTCAAACG-1

using 120 reads

====================================================================================

graph has 56 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 1[117]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.556 = GGGAGATTCTGATACG-1

using 23307 reads

====================================================================================

graph has 13406 edges initially, 280 edges after simplification

total ucounts = 918
nonsolo ucounts = 701[2^95, 3^63, 4^57, 5^48, 6^27, 7^29, 8^26, 9^28, 10^23, 11^23, 12^13, 13^15, 14^14, 15^17, 16^12, 17^12, 18^12, 19^14, 20^13, 21^10, 22^15, 23^13, 24^11, 25^7, 26^6, 27^12, 28^8, 29^7, 30^7, 31^2, 32^4, 33^3, 34^3, 35^5, 36^2, 37, 38^3, 39, 40^5, 41^4, 43, 44, 45^2, 49, 51^2, 55^2, 57^2, 58, 68^2, 71, 74, 97, 110, 119, 128, 201, 305, 462, 863, 1134, 1185, 1492, 1509, 1782, 2033, 3097]
surviving nonsolo ucounts = 11[201, 305, 462, 863, 1134, 1185, 1492, 1509, 1782, 2033, 3097]
ids = [140, 608, 575, 224, 654, 792, 419, 282, 495, 787, ...]

====================================================================================

UMI info for barcode GGGAGATTCTGATACG-1 contig 1 = TTCCTCTGCT...
umi CCAGCTGGCC = 1498 reads: +382 validated
umi CTGCCACAGC = 1492 reads: +382 validated
umi GATCGCTTGG = 1789 reads: +382 validated
umi GGTATGAGCA = 456 reads: +382 validated
umi TGAGTCTTTT = 2101 reads: -1XX +1 -2XX +2 -15XX +2 -3XX +2 -3XX +1 -10XX +340 invalidated

GOOD CONTIGS

TIG 1[bases=546]
0-112 ==> 0-112 on |618|TRBV30|5'UTR| [len=112] (mis=0)
112-447 ==> 0-335 on |619|TRBV30|L-REGION+V-REGION| [len=335] (mis=2)
451-494 ==> 8-51 on |539|TRBJ1-4|J-REGION| [len=51] (mis=0)
494-546 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 5 umis using 443 reads
cdr3 = CAWSRQEKLFF at 433, score = 7 + 8
umis assigned: [282, 419, 495, 575, 787]
of which 5 are surviving nonsolos
reads assigned: 7131
start codons at 109, 112, 180
confident = true

REJECT CONTIGS

TIG 1[bases=657]
4-169 ==> 7189-7354 on segment before TRBV5-6 exon 1 [len=7354] (mis=0)
49-210 ==> 7801-7962 on segment before TRBV5-7 exon 1 [len=7962] (mis=12)
210-553 ==> 0-343 on |643|TRBV5-6|L-REGION+V-REGION| [len=343] (mis=2)
555-605 ==> 0-50 on |540|TRBJ1-5|J-REGION| [len=50] (mis=4)
605-657 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
umis assigned: [140, 224, 608, 654, 699]
of which 5 are surviving nonsolos
reads assigned: 5385
start codons at 76, 183, 210, 332, 352, 415, 496, 581
confident = false
did not find CDR3
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.560 = GGGAGATTCTTGCAAG-1

using 102 reads

====================================================================================

graph has 106 edges initially, 4 edges after simplification

total ucounts = 8
nonsolo ucounts = 6[4, 10, 14, 17, 26, 29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.561 = GGGATGAAGAAGATTC-1

using 66 reads

====================================================================================

graph has 60 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 5[2, 5, 6^2, 43]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.562 = GGGATGAAGAAGGACA-1

using 54349 reads

====================================================================================

graph has 17643 edges initially, 250 edges after simplification

total ucounts = 730
nonsolo ucounts = 510[2^72, 3^45, 4^43, 5^26, 6^25, 7^20, 8^15, 9^19, 10^14, 11^23, 12^15, 13^12, 14^11, 15^4, 16^16, 17^10, 18^12, 19^13, 20^5, 21^6, 22^5, 23^8, 24^9, 25^8, 26^6, 27^5, 28, 29^2, 30^3, 31^2, 33^4, 34^3, 35^5, 36^2, 37^3, 38^2, 39, 43^2, 44^2, 47, 48^2, 50, 53^3, 56^2, 58^2, 65, 67^2, 76, 77, 78, 109, 114, 149, 1005, 1124, 1447, 1457, 1525, 1725, 4384, 7769, 8276, 9241, 9454]
surviving nonsolo ucounts = 11[1005, 1124, 1447, 1457, 1525, 1725, 4384, 7769, 8276, 9241, 9454]
ids = [119, 281, 51, 690, 381, 495, 285, 706, 185, 39, ...]

====================================================================================

UMI info for barcode GGGATGAAGAAGGACA-1 contig 1 = GAGAAGCCTC...
umi CCGGCCATCG = 1133 reads: +400 validated
umi CCTAACTCCT = 4411 reads: -13X +1 -2XX +384 invalidated

UMI info for barcode GGGATGAAGAAGGACA-1 contig 2 = AGCAGATCTC...
umi AATCTGATGG = 1445 reads: +394 validated
umi AGGCCACATT = 1013 reads: +394 validated
umi TTCTACCTGC = 1455 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=559]
0-117 ==> 0-117 on |474|TRAV19|5'UTR| [len=117] (mis=1)
117-467 ==> 0-350 on |475|TRAV19|L-REGION+V-REGION| [len=350] (mis=4)
461-517 ==> 7-63 on |407|TRAJ22|J-REGION| [len=63] (mis=0)
517-559 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 2 umis using 325 reads
cdr3 = CALRGSARQLTF at 450, score = 7 + 9
umis assigned: [281, 285]
of which 2 are surviving nonsolos
reads assigned: 5425
start codons at 111, 117, 174, 229, 250, 340, 349
confident = true

TIG 2[bases=543]
0-97 ==> 15-112 on |618|TRBV30|5'UTR| [len=112] (mis=0)
97-432 ==> 0-335 on |619|TRBV30|L-REGION+V-REGION| [len=335] (mis=2)
443-491 ==> 0-48 on |548|TRBJ2-5|J-REGION| [len=48] (mis=2)
491-543 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 3 umis using 211 reads
cdr3 = CAWSRLAGGQETQYF at 418, score = 7 + 7
umis assigned: [51, 119, 690]
of which 3 are surviving nonsolos
reads assigned: 3840
start codons at 94, 97, 165
confident = true

REJECT CONTIGS

TIG 1[bases=330]
4-211 ==> 5793-6000 on segment before TRBD1 exon 1 [len=6000] (mis=0)
228-278 ==> 0-50 on |540|TRBJ1-5|J-REGION| [len=50] (mis=1)
278-330 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
umis assigned: [39, 185, 477, 706]
of which 4 are surviving nonsolos
reads assigned: 34162
start codons at 88, 98, 172, 254
confident = false
did not find CDR3

TIG 2[bases=495]
5-42 ==> 11323-11360 on segment before TRAV21 exon 1 [len=11360] (mis=0)
49-385 ==> 0-336 on |481|TRAV21|L-REGION+V-REGION| [len=336] (mis=2)
387-453 ==> 0-66 on |432|TRAJ45|J-REGION| [len=66] (mis=0)
453-495 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
umis assigned: [264, 381, 495]
of which 2 are surviving nonsolos
reads assigned: 3201
start codons at 49, 93, 293
confident = false
frameshifted full length stopped transcript of length 495
did not find CDR3
now this is a cell
paired!

GCCTCACAAGTCGTGGACTCAGCAGTATACTTCTGTGCTCTGCGTGGTTCTGCAAGGCAACTGACCTTTGGATCTGGGACACAATTGACTGTTTTACCTG <==> CTTCTCAGTGACTCTGGCTTCTATCTCTGTGCCTGGAGTAGGTTAGCGGGAGGGCAAGAGACCCAGTACTTCGGGCCAGGCACGCGGCTCCTGGTGCTCG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.569 = GGGATGAAGAGAGCTC-1

using 33110 reads

====================================================================================

graph has 10925 edges initially, 158 edges after simplification

total ucounts = 476
nonsolo ucounts = 339[2^56, 3^23, 4^26, 5^12, 6^14, 7^18, 8^11, 9^17, 10^7, 11^11, 12^9, 13^7, 14^8, 15^9, 16^5, 17^4, 18^10, 19^5, 20^5, 21^6, 22^4, 23^6, 24^5, 25^3, 26^7, 27, 28, 29, 30^3, 31, 32, 33^2, 34^2, 35, 37^3, 39, 40, 41^2, 47^2, 48, 59, 60^2, 62, 63, 71, 86, 90, 96^2, 108, 115, 320, 1154, 1196, 1290, 1566, 1640, 1655, 1679, 1819, 1867, 2062, 2218, 2321, 2375, 2668, 2672]
surviving nonsolo ucounts = 16[320, 1154, 1196, 1290, 1566, 1640, 1655, 1679, 1819, 1867, 2062, 2218, 2321, 2375, 2668, 2672]
ids = [162, 118, 318, 262, 1, 17, 197, 421, 204, 309, ...]

====================================================================================

UMI info for barcode GGGATGAAGAGAGCTC-1 contig 1 = GGGACAGTGA...
umi AAACAAGTAC = 1571 reads: +403 validated
umi AACATACGGC = 1651 reads: +403 validated
umi ACATATTCCT = 2676 reads: +403 validated
umi ACTGTTTTTA = 2398 reads: +403 validated
umi ATTGGTGTTA = 2677 reads: +403 validated
umi CACGCTTCTG = 1653 reads: +403 validated
umi CATACTGCAT = 1829 reads: +403 validated
umi CGGGTAGCTG = 1294 reads: +403 validated
umi CTTGTTGTTT = 1877 reads: +403 validated
umi GCACCATTCT = 2217 reads: +403 validated
umi GGCGTTAGTC = 2340 reads: +403 validated
umi TATTCTTCAG = 1699 reads: +353 -2XX +1 -1XX +46 invalidated

UMI info for barcode GGGATGAAGAGAGCTC-1 contig 2 = TTTTGAAACC...
umi AGGCTTAGGT = 1117 reads: +380 -1XX +1 -1XX +1 -2XX +1 -3XX +7 invalidated
umi ATGTACCCAA = 295 reads: +397 validated
umi GAGAGTTTCC = 1187 reads: +397 validated

GOOD CONTIGS

TIG 1[bases=505]
0-50 ==> 0-50 on |678|TRBV7-3|5'UTR| [len=50] (mis=3)
50-397 ==> 0-347 on |679|TRBV7-3|L-REGION+V-REGION| [len=347] (mis=2)
404-453 ==> 0-49 on |546|TRBJ2-3|J-REGION| [len=49] (mis=2)
453-505 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 12 umis using 1560 reads
cdr3 = CASSLAGGGTDTQYF at 380, score = 8 + 8
umis assigned: [1, 17, 65, 93, 178, 197, 204, 262, 309, 330] and 2 others
of which 12 are surviving nonsolos
reads assigned: 23424
start codons at 50, 159, 273
confident = true

TIG 2[bases=567]
0-128 ==> 0-128 on |523|TRAV8-4|5'UTR| [len=128] (mis=0)
128-469 ==> 0-341 on |524|TRAV8-4|L-REGION+V-REGION| [len=341] (mis=6)
462-525 ==> 0-63 on |425|TRAJ39|J-REGION| [len=63] (mis=5)
525-567 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 2 umis using 38 reads
cdr3 = CAVGPNAGNMLTF at 455, score = 7 + 9
umis assigned: [118, 162, 318]
of which 3 are surviving nonsolos
reads assigned: 2550
start codons at 128, 291, 431, 471, 482, 512
confident = true

REJECT CONTIGS

TIG 1[bases=437]
15-350 ==> 0-335 on |615|TRBV29-1|L-REGION+V-REGION| [len=335] (mis=4)
370-417 ==> 0-47 on |550|TRBJ2-7|J-REGION| [len=47] (mis=5)
cdr3 = CSVVWTPVEVAGETSSTSGRAP at 336, score = 7 + 5
umis assigned: [356]
of which 1 are surviving nonsolos
reads assigned: 2015
start codons at 15, 150, 153, 229, 306
confident = false
not full
frameshifted full length transcript of length 437
VJ delta = 26
not full
now this is a cell
paired!

GCCCATATGAGCGACGCGGCTGAGTACTTCTGTGCTGTGGGTCCCAATGCAGGCAACATGCTCACCTTTGGAGGGGGAACAAGGTTAATGGTCAAACCCC <==> GAGCGGGGGGACTCAGCCGTGTATCTCTGTGCCAGCAGCTTGGCGGGGGGCGGTACAGATACGCAGTATTTTGGCCCAGGCACCCGGCTGACAGTGCTCG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.570 = GGGATGAAGAGCTTCT-1

using 61 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[61]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.571 = GGGATGAAGAGGTTAT-1

using 20 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.572 = GGGATGAAGATAGGAG-1

using 90 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[90]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.577 = GGGATGAAGATTACCC-1

using 4792 reads

====================================================================================

graph has 3884 edges initially, 114 edges after simplification

total ucounts = 531
nonsolo ucounts = 398[2^43, 3^32, 4^35, 5^27, 6^18, 7^26, 8^29, 9^20, 10^14, 11^13, 12^14, 13^12, 14^9, 15^10, 16^7, 17^12, 18^13, 19^8, 20^6, 21^3, 22^4, 23^4, 24^3, 25^2, 27^2, 28^3, 29^5, 30^5, 31^3, 33, 37, 41, 45, 46, 48, 50, 54, 57, 60, 61, 62, 67, 77, 80, 86]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.580 = GGGATGAAGCCAGGAT-1

using 76 reads

====================================================================================

graph has 58 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[76]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.582 = GGGATGAAGCGAAGGG-1

using 31 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.583 = GGGATGAAGCGCCTTG-1

using 52 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[52]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.586 = GGGATGAAGCTACCTA-1

using 34 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.587 = GGGATGAAGCTGCAAG-1

using 33 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.591 = GGGATGAAGGACTGGT-1

using 67 reads

====================================================================================

graph has 56 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 6[2^2, 4^2, 11, 44]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.593 = GGGATGAAGGAGTACC-1

using 64 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[64]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.595 = GGGATGAAGGGAGTAA-1

using 23785 reads

====================================================================================

graph has 8627 edges initially, 100 edges after simplification

total ucounts = 368
nonsolo ucounts = 235[2^43, 3^21, 4^17, 5^10, 6^11, 7^5, 8^5, 9^7, 10^3, 11^7, 12^8, 13^9, 14^5, 15^2, 16^7, 17^5, 18^5, 19^3, 20^5, 21, 22^3, 23^4, 24^6, 25, 26^3, 27, 28, 29^4, 30^3, 34^4, 35^2, 36, 37, 38, 44, 49, 50, 53, 56, 63, 68, 86, 96, 111, 151, 275, 758, 1093, 1119, 1491, 1531, 1587, 2347, 2424, 7861]
surviving nonsolo ucounts = 10[275, 758, 1093, 1119, 1491, 1531, 1587, 2347, 2424, 7861]
ids = [233, 164, 302, 291, 192, 358, 106, 329, 264, 214]

====================================================================================

UMI info for barcode GGGATGAAGGGAGTAA-1 contig 1 = ATATATGGGG...
umi CATGAAGGCA = 1608 reads: +400 validated
umi CTCTTCGGTT = 1498 reads: +400 validated
umi GATTCTAGCC = 8145 reads: +76 -3XX +1 -20XX +4 -104XX +1 -1X +1 -1X +1 -8XX +1 -1XX +1 -6XX +2 -1XX +1 -3XX +163 invalidated
umi TACAAGGCAT = 2412 reads: +400 validated
umi TGGGTCCCCC = 2350 reads: +400 validated
umi TTTATACAGC = 1523 reads: +400 validated

GOOD CONTIGS

TIG 1[bases=510]
58-405 ==> 0-347 on |687|TRBV7-8|L-REGION+V-REGION| [len=347] (mis=1)
410-458 ==> 0-48 on |536|TRBJ1-1|J-REGION| [len=48] (mis=1)
458-510 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 6 umis using 1413 reads
cdr3 = CASSLDRGNTEAFF at 388, score = 8 + 8
umis assigned: [106, 192, 214, 264, 329, 358]
of which 6 are surviving nonsolos
reads assigned: 16920
start codons at 4, 58, 167, 200, 266
confident = true

REJECT CONTIGS

TIG 1[bases=494]
0-74 ==> 132-206 on segment before TRBV23-1 exon 2 [len=206] (mis=0)
71-372 ==> 46-347 on |601|TRBV23-1|L-REGION+V-REGION| [len=347] (mis=1)
395-442 ==> 0-47 on |550|TRBJ2-7|J-REGION| [len=47] (mis=2)
442-494 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
umis assigned: [164, 233, 291, 302]
of which 4 are surviving nonsolos
reads assigned: 2750
start codons at 83, 142, 211, 233, 259, 285
confident = false
did not find CDR3
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.597 = GGGATGAAGGGATGGG-1

using 93 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[22, 70]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.599 = GGGATGAAGGGTGTTG-1

using 24 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.603 = GGGATGAAGTGACTCT-1

using 49 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[49]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.609 = GGGATGAAGTTACGGG-1

using 39336 reads

====================================================================================

graph has 11700 edges initially, 92 edges after simplification

total ucounts = 421
nonsolo ucounts = 280[2^59, 3^37, 4^25, 5^15, 6^9, 7^11, 8^10, 9^13, 10^7, 11^8, 12^4, 13^7, 14^6, 15^11, 16^2, 17^2, 18^3, 19^4, 20^4, 21^3, 22^2, 23, 24, 25, 26, 27, 28, 29^2, 31^3, 32, 33, 43, 46, 48, 50, 61, 81, 84, 120, 143, 821, 907, 1046, 1106, 1200, 1206, 1533, 1740, 1911, 2238, 2420, 2505, 2996, 4614, 5106, 5140]
surviving nonsolo ucounts = 16[821, 907, 1046, 1106, 1200, 1206, 1533, 1740, 1911, 2238, 2420, 2505, 2996, 4614, 5106, 5140]
ids = [163, 345, 166, 28, 411, 237, 37, 45, 410, 68, ...]

====================================================================================

UMI info for barcode GGGATGAAGTTACGGG-1 contig 1 = ACAGTGATCC...
umi ACAGTAATAC = 1735 reads: +403 validated
umi ACTTGCCGCT = 2242 reads: +403 validated
umi CCGCGGGCCT = 220 reads: -368 +2 -2XX +2 -1XX +2 -4XX +3 -1XX +1 -1XX +5 -1XX +2 -1XX +2 -4XX +1 invalidated
umi CCGTGAAGGT = 1047 reads: +403 validated
umi CTTGGCTACT = 3005 reads: +403 validated
umi GCATTCGTGC = 1237 reads: +76 -1XX +326 invalidated
umi GTGCACAACC = 4635 reads: -173X +230 invalidated
umi TCTGTGTCTA = 913 reads: +403 validated
umi TCTTAATCGA = 2517 reads: +403 validated
umi TTCCGTCCGG = 5118 reads: -228X +175 invalidated
umi TTTAATCAAT = 1894 reads: +403 validated
umi TTTAGAGGCC = 1208 reads: +403 validated

UMI info for barcode GGGATGAAGTTACGGG-1 contig 2 = GAGATATAAG...
umi AAGCAACCAA = 2437 reads: +391 validated
umi AATAATATTG = 1116 reads: +391 validated
umi AATCGTGATC = 1517 reads: +391 validated

GOOD CONTIGS

TIG 1[bases=502]
0-47 ==> 0-47 on |674|TRBV7-2|5'UTR| [len=47] (mis=0)
47-394 ==> 0-347 on |675|TRBV7-2|L-REGION+V-REGION| [len=347] (mis=1)
403-450 ==> 0-47 on |550|TRBJ2-7|J-REGION| [len=47] (mis=2)
450-502 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 11 umis using 1667 reads
cdr3 = CASSLGGRGLYEQYF at 377, score = 8 + 8
umis assigned: [45, 68, 163, 166, 208, 237, 273, 345, 346, 390] and 2 others
of which 12 are surviving nonsolos
reads assigned: 25257
start codons at 47, 156
confident = true

TIG 2[bases=478]
45-381 ==> 0-336 on |481|TRAV21|L-REGION+V-REGION| [len=336] (mis=3)
374-436 ==> 0-62 on |423|TRAJ37|J-REGION| [len=62] (mis=3)
436-478 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 3 umis using 311 reads
cdr3 = CAVSGNTGKLIF at 369, score = 9 + 8
umis assigned: [23, 28, 37]
of which 3 are surviving nonsolos
reads assigned: 4973
start codons at 45, 89, 289
confident = true

REJECT CONTIGS

TIG 1[bases=385]
5-282 ==> 60-337 on |464|TRAV13-1|L-REGION+V-REGION| [len=337] (mis=1)
288-343 ==> 8-63 on |401|TRAJ17|J-REGION| [len=63] (mis=0)
343-385 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
umis assigned: [148]
of which 1 are surviving nonsolos
reads assigned: 5060
start codons at 9, 159
confident = false
did not find CDR3
now this is a cell
paired!

GCTTCTCAGCCTGGTGACTCAGCCACCTACCTCTGTGCTGTGTCTGGCAACACAGGCAAACTAATCTTTGGGCAAGGGACAACTTTACAAGTAAAACCAG <==> CAGCAGGAGGACTCGGCCGTGTATCTCTGTGCCAGCAGCTTAGGGGGGCGGGGCCTCTACGAGCAGTACTTCGGGCCGGGCACCAGGCTCACGGTCACAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.611 = GGGATGAAGTTCGCAT-1

using 50234 reads

====================================================================================

graph has 20093 edges initially, 325 edges after simplification

total ucounts = 1406
nonsolo ucounts = 985[2^156, 3^111, 4^77, 5^63, 6^67, 7^47, 8^55, 9^39, 10^33, 11^25, 12^31, 13^30, 14^27, 15^22, 16^24, 17^13, 18^16, 19^14, 20^13, 21^12, 22^13, 23^5, 24^7, 25^9, 26^6, 27^5, 28^5, 29^3, 30, 32, 33, 34^3, 36^3, 38^2, 39, 42, 44^2, 45, 47, 48, 49^3, 52, 53, 54, 57^2, 65, 68, 71, 76, 77, 78, 82, 84, 91, 92, 101, 105, 107, 113, 162, 353, 928, 986, 1074, 1171, 1331, 1477, 1501, 1524, 1707, 1772, 1826, 2389, 2896, 5952, 12596]
surviving nonsolo ucounts = 14[353, 986, 1171, 1331, 1477, 1501, 1524, 1707, 1772, 1826, 2389, 2896, 5952, 12596]
ids = [342, 996, 528, 1051, 519, 885, 870, 951, 549, 849, ...]

====================================================================================

UMI info for barcode GGGATGAAGTTCGCAT-1 contig 1 = GTTCCCCTAT...
umi ATACCCTTCT = 2388 reads: +400 validated
umi ATGGTCCACT = 2718 reads: -322X +1 -7XX +1 -1XX +3 -8XX +2 -2XX +1 -2XX +2 -3XX +45 invalidated
umi ATTATGTTTC = 312 reads: -384 +2 -1XX +2 -2XX +1 -2XX +1 -1XX +2 -1XX +1 invalidated
umi CCCCAGGTTA = 1462 reads: +400 validated
umi CCCGCTACCA = 1091 reads: -332 +3 -8XX +2 -2XX +1 -2XX +2 -3XX +45 invalidated
umi CCGCCCTCGT = 1775 reads: +400 validated
umi CTCGGACAGA = 278 reads: -388 +1 -2XX +2 -1XX +1 -1XX +2 -1XX +1 invalidated
umi GAATATTCCT = 1 reads: -399X +1 invalidated
umi GCGTCCCGCT = 1786 reads: +400 validated
umi GGAGCAGGTG = 1506 reads: +400 validated
umi GGCACTCATC = 1 reads: -400X invalidated
umi GTCAGTATCT = 1695 reads: +400 validated
umi GTTTTATGCC = 902 reads: -379X +1 -5X +1 -9XX +1 -2XX +2 invalidated
umi TCGTAACGTT = 2 reads: -400X invalidated
umi TTGAGTGTCT = 5107 reads: -378 +2 -1XX +2 -1XX +2 -1XX +2 -2XX +1 -2XX +1 -1XX +2 -1XX +1 invalidated

UMI info for barcode GGGATGAAGTTCGCAT-1 contig 2 = GGAAGAAGCT...
umi ACCGCCAGCT = 1 reads: -364X +1 -4X +1 -6X invalidated
umi ACGATACATA = 10382 reads: -341 +6 -1XX +1 -1XX +3 -1XX +2 -4XX +2 -1XX +5 -1XX +2 -1XX +2 -2XX invalidated
umi GGCGTAGCAC = 1496 reads: +376 validated
umi TAGTTTCGTG = 1167 reads: +376 validated

GOOD CONTIGS

TIG 1[bases=509]
0-57 ==> 0-57 on |647|TRBV6-1|5'UTR| [len=57] (mis=0)
57-401 ==> 0-344 on |648|TRBV6-1|L-REGION+V-REGION| [len=344] (mis=1)
407-457 ==> 0-50 on |540|TRBJ1-5|J-REGION| [len=50] (mis=5)
457-509 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 6 umis using 658 reads
cdr3 = CASSEGRGSYQPQHF at 384, score = 9 + 8
umis assigned: [292, 328, 342, 519, 528, 549, 702, 767, 849, 870] and 5 others
of which 11 are surviving nonsolos
reads assigned: 20792
start codons at 15, 57, 115, 168, 192, 207, 234, 298, 307, 433
confident = true

TIG 2[bases=477]
0-59 ==> 17-76 on |487|TRAV25|5'UTR| [len=76] (mis=0)
59-380 ==> 0-321 on |488|TRAV25|L-REGION+V-REGION| [len=327] (mis=1)
381-435 ==> 0-54 on |430|TRAJ43|J-REGION| [len=54] (mis=2)
435-477 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 2 umis using 145 reads
cdr3 = CLNNNDMRF at 377, score = 6 + 8
umis assigned: [159, 176, 885, 1051]
of which 3 are surviving nonsolos
reads assigned: 12985
start codons at 41, 59, 77, 88, 92, 111, 125, 147, 360, 390, 395
confident = true
now this is a cell
paired!

CACATCACAGCCACCCAGACTACAGATGTAGGAACCTACTTCTGTCTGAATAACAATGACATGCGCTTTGGAGCAGGGACCAGACTGACAGTAAAACCAA <==> GCTCCCTCCCAGACATCTGTGTACTTCTGTGCCAGCAGTGAAGGGCGGGGGTCGTATCAGCCCCAGCATTTTGGTGATGGGACTCGACTCTCCATCCTAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.617 = GGGATGACAACACCTA-1

using 68 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[68]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.619 = GGGATGACAAGGACAC-1

using 12313 reads

====================================================================================

graph has 6232 edges initially, 68 edges after simplification

total ucounts = 349
nonsolo ucounts = 261[2^42, 3^17, 4^17, 5^23, 6^16, 7^12, 8^9, 9^11, 10^9, 11^8, 12^10, 13^10, 14^5, 15^7, 16^7, 17^3, 18^4, 19, 20^4, 21^6, 22^2, 23^2, 25^2, 26, 27^3, 28^2, 29^4, 30, 31, 32, 37, 38, 41, 42, 44^3, 45, 47, 48, 53, 61, 72, 73, 81, 85, 93, 105, 583, 2012, 6333]
surviving nonsolo ucounts = 1[6333]
ids = [284]

====================================================================================

UMI info for barcode GGGATGACAAGGACAC-1 contig 1 = GACTGTGATT...
umi CGCGCCTTTT = 662 reads: -366X +2 -1XX +3 -2XX +1 -1XX +1 -4XX +2 -1XX +2 -2XX +3 invalidated
umi TCGCTGCCTT = 6369 reads: +391 validated

GOOD CONTIGS

TIG 1[bases=489]
0-56 ==> 0-56 on |529|TRAV9-2|5'UTR| [len=56] (mis=1)
56-394 ==> 0-338 on |530|TRAV9-2|L-REGION+V-REGION| [len=338] (mis=2)
390-447 ==> 0-57 on |405|TRAJ20|J-REGION| [len=57] (mis=2)
447-489 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 1 umis using 368 reads
cdr3 = CALSANDYKLSF at 380, score = 9 + 8
umis assigned: [125, 284]
of which 1 are surviving nonsolos
reads assigned: 6885
start codons at 15, 56, 131, 219, 273
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.621 = GGGATGACAATCGAAA-1

using 80 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[2, 77]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.623 = GGGATGACAATTCCTT-1

using 103 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[102]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.624 = GGGATGACAATTGCTG-1

using 70 reads

====================================================================================

graph has 80 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 6[3, 4, 5, 12, 13, 32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.626 = GGGATGACACAGGCCT-1

using 2224 reads

====================================================================================

graph has 2336 edges initially, 70 edges after simplification

total ucounts = 316
nonsolo ucounts = 217[2^40, 3^25, 4^22, 5^20, 6^10, 7^11, 8^12, 9^5, 10^5, 11^7, 12^10, 13^8, 14^7, 15^2, 16, 17^2, 18^2, 19^4, 20^3, 22^2, 23^2, 24, 25^2, 27^2, 28^2, 33, 37, 38, 39, 47, 51, 53, 74^2, 83]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.627 = GGGATGACACATGACT-1

using 80 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[80]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.629 = GGGATGACACTTAACG-1

using 40 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[39]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.631 = GGGATGACAGATAATG-1

using 149 reads

====================================================================================

graph has 92 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[149]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.635 = GGGATGACAGGCTCAC-1

using 412 reads

====================================================================================

graph has 232 edges initially, 8 edges after simplification

total ucounts = 20
nonsolo ucounts = 17[2, 3^3, 4, 5, 9^4, 16, 17, 38, 48^2, 64, 122]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.637 = GGGATGACAGGTCGTC-1

using 38 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.638 = GGGATGACAGGTCTCG-1

using 36 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.640 = GGGATGACAGTACACT-1

using 96 reads

====================================================================================

graph has 74 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 6[2^2, 4, 5, 6, 75]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.642 = GGGATGACAGTTTACG-1

using 59 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[59]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.643 = GGGATGACATACAGCT-1

using 96 reads

====================================================================================

graph has 76 edges initially, 6 edges after simplification

total ucounts = 6
nonsolo ucounts = 5[2^2, 20, 26, 45]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.646 = GGGATGACATACTACG-1

using 75 reads

====================================================================================

graph has 74 edges initially, 4 edges after simplification

total ucounts = 11
nonsolo ucounts = 9[2^2, 3^3, 5, 7, 12, 36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.651 = GGGATGACATGAACCT-1

using 3146 reads

====================================================================================

graph has 1097 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 3[5, 11, 3126]
surviving nonsolo ucounts = 1[3126]
ids = [2]

====================================================================================

UMI info for barcode GGGATGACATGAACCT-1 contig 1 = TGGGGGAGAT...
umi ATATCTTTAT = 3119 reads: +397 validated

GOOD CONTIGS

TIG 1[bases=465]
16-359 ==> 0-343 on |693|TRBV9|L-REGION+V-REGION| [len=343] (mis=1)
364-413 ==> 0-49 on |546|TRBJ2-3|J-REGION| [len=49] (mis=1)
413-465 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 1 umis using 262 reads
cdr3 = CASSVQGITDTQYF at 343, score = 9 + 8
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 3065
start codons at 16, 138, 224
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.654 = GGGATGACATGATCCA-1

using 96 reads

====================================================================================

graph has 62 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[96]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.655 = GGGATGACATGCCCGA-1

using 184 reads

====================================================================================

graph has 184 edges initially, 2 edges after simplification

total ucounts = 60
nonsolo ucounts = 10[2, 3, 4, 6, 8, 14, 15^2, 17, 50]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.656 = GGGATGACATGGAATA-1

using 120 reads

====================================================================================

graph has 114 edges initially, 4 edges after simplification

total ucounts = 11
nonsolo ucounts = 7[3, 5, 6, 12, 14, 37, 39]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.664 = GGGATGAGTACCATCA-1

using 41 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[40]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.669 = GGGATGAGTAGCACGA-1

using 73 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[25, 48]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.682 = GGGATGAGTCCGACGT-1

using 35 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[17, 18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.683 = GGGATGAGTCCGTTAA-1

using 128 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[128]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.689 = GGGATGAGTCTGGAGA-1

using 73 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[73]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.692 = GGGATGAGTGATGTCT-1

using 60 reads

====================================================================================

graph has 48 edges initially, 4 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[9, 19, 30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.707 = GGGATGAGTTCTCATT-1

using 173 reads

====================================================================================

graph has 88 edges initially, 4 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[60, 113]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.718 = GGGATGATCATCGATG-1

using 30 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.723 = GGGATGATCATGTCCC-1

using 20 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[3, 17]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.724 = GGGATGATCATGTGGT-1

using 78 reads

====================================================================================

graph has 116 edges initially, 4 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[28, 49]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.733 = GGGATGATCGCGCCAA-1

using 50 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[7, 18, 24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.737 = GGGATGATCGGCGCTA-1

using 5815 reads

====================================================================================

graph has 931 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 3[2^2, 5806]
surviving nonsolo ucounts = 1[5806]
ids = [3]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.738 = GGGATGATCGGTCTAA-1

using 52 reads

====================================================================================

graph has 48 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 1[48]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.740 = GGGATGATCGTGGACC-1

using 223 reads

====================================================================================

graph has 264 edges initially, 6 edges after simplification

total ucounts = 17
nonsolo ucounts = 11[2, 3, 5^2, 6^2, 19, 25, 41, 44, 61]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.742 = GGGATGATCTATCCCG-1

using 50 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[50]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.745 = GGGATGATCTGTACGA-1

using 35 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.747 = GGGATGATCTTGAGAC-1

using 33 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.749 = GGGATGATCTTTACAC-1

using 88 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[88]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.759 = GGGCACTAGACTTTCG-1

using 127 reads

====================================================================================

graph has 146 edges initially, 4 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[15, 112]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.764 = GGGCACTAGATATGCA-1

using 65 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[65]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.772 = GGGCACTAGCCGCCTA-1

using 61 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[61]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.775 = GGGCACTAGCGGCTTC-1

using 71 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 5[2^2, 3, 10, 51]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.777 = GGGCACTAGCTAACTC-1

using 70 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[70]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.779 = GGGCACTAGCTCCCAG-1

using 127 reads

====================================================================================

graph has 54 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 3[9, 10, 105]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.780 = GGGCACTAGCTCCTCT-1

using 52 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[52]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.782 = GGGCACTAGCTGAAAT-1

using 43 reads

====================================================================================

graph has 52 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 5[2^2, 3, 7, 23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.783 = GGGCACTAGCTGAACG-1

using 101 reads

====================================================================================

graph has 80 edges initially, 2 edges after simplification

total ucounts = 13
nonsolo ucounts = 5[4, 6, 12, 25, 46]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.784 = GGGCACTAGCTGCAAG-1

using 122 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[122]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.790 = GGGCACTAGGCAGTCA-1

using 42 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[42]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.792 = GGGCACTAGGCTAGGT-1

using 472 reads

====================================================================================

graph has 352 edges initially, 14 edges after simplification

total ucounts = 23
nonsolo ucounts = 19[2^2, 3^2, 4, 5, 6, 7, 9, 17, 21, 22, 26, 30, 32, 48, 52, 77, 102]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.793 = GGGCACTAGGCTATCT-1

using 60 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[60]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.794 = GGGCACTAGGGCTTCC-1

using 36 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 5[2^3, 9, 19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.801 = GGGCACTAGTACTTGC-1

using 42 reads

====================================================================================

graph has 54 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 8[2^3, 3, 5, 7, 9^2]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.808 = GGGCACTAGTGGAGTC-1

using 57 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[57]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.810 = GGGCACTAGTTAGGTA-1

using 19 reads

====================================================================================

graph has 6 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.812 = GGGCACTAGTTTCCTT-1

using 24 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 65.817 = GGGCACTCAACACCTA-1

using 23598 reads

====================================================================================

graph has 12583 edges initially, 330 edges after simplification

total ucounts = 1990
nonsolo ucounts = 1424[2^241, 3^158, 4^148, 5^121, 6^98, 7^85, 8^87, 9^55, 10^39, 11^35, 12^36, 13^29, 14^35, 15^26, 16^27, 17^16, 18^18, 19^12, 20^16, 21^12, 22^12, 23^13, 24^7, 25^6, 26^6, 27^6, 28^5, 29^6, 30^5, 31^3, 32^3, 33^4, 35^7, 36^4, 37^2, 39^3, 40^2, 42^3, 43^3, 44^3, 45^2, 47^2, 48^2, 50, 51, 52, 55, 56^2, 60, 62, 63, 64, 66, 67, 68, 69, 75, 80, 82, 99, 233, 3807, 5686]
surviving nonsolo ucounts = 4[42, 233, 3807, 5686]
ids = [425, 962, 1312, 457]

====================================================================================
NOT paired!
sorting bam, mem = 0.11
running samtools sort -l 8G -o /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_T_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk65-ucc6a7a72e6/files/contig_bam_sorted.bam /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_T_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk65-ucc6a7a72e6/files/contig_bam.bam

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

67.853 seconds used processing barcodes, peak mem = 0.27
