[stdout]

starting vdj_asm_asm, mem = 0.01
available mem = 830.59 GB
/scratch/etanis/software/cellranger-6.1.2/lib/bin/cr_vdj martian assembly main /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_T_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk35-ucc6a7a72e6 /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_T_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk35-ucc6a7a72e6/files /scratch/etanis/ST-118/multi/dimitri-multi/journal/SC_MULTI_CS.SC_MULTI_CORE.MULTI_GEM_WELL_PROCESSOR.VDJ_T_GEM_WELL_PROCESSOR.SC_VDJ_CONTIG_ASSEMBLER.ASSEMBLE_VDJ.fork0.chnk35.ucc6a7a72e6
n50_n50_rpu = 2159

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.0 = CGAATGTCATGTTCCC-1

using 8468 reads

====================================================================================

graph has 8072 edges initially, 214 edges after simplification

total ucounts = 1225
nonsolo ucounts = 830[2^148, 3^101, 4^68, 5^65, 6^50, 7^36, 8^40, 9^45, 10^30, 11^31, 12^21, 13^18, 14^14, 15^20, 16^13, 17^12, 18^16, 19^13, 20^13, 21^10, 22^7, 23^6, 24^3, 25^3, 26^5, 27, 28^5, 29^3, 30, 31, 32, 33^3, 34, 36^2, 39, 40^3, 42^2, 43, 45, 47, 49^2, 51^2, 55^2, 62, 69, 72^2, 77, 96, 97, 99, 106]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.4 = CGAATGTCATTGCGGC-1

using 138 reads

====================================================================================

graph has 120 edges initially, 4 edges after simplification

total ucounts = 7
nonsolo ucounts = 3[2, 37, 95]
surviving nonsolo ucounts = 1[95]
ids = [2]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.5 = CGAATGTGTAAATACG-1

using 35 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.7 = CGAATGTGTACATGTC-1

using 75 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[75]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.10 = CGAATGTGTAGAAAGG-1

using 15 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.12 = CGAATGTGTAGCGTAG-1

using 86 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[85]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.15 = CGAATGTGTAGCTTGT-1

using 69 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 3[7, 14, 42]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.17 = CGAATGTGTATAGGGC-1

using 501 reads

====================================================================================

graph has 252 edges initially, 8 edges after simplification

total ucounts = 66
nonsolo ucounts = 45[2^7, 3^2, 5^4, 6^2, 7^4, 8^3, 9^3, 10^3, 11^3, 12^3, 14^2, 15^2, 16, 20, 23, 25, 29, 31, 48]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.24 = CGAATGTGTCTAACGT-1

using 135 reads

====================================================================================

graph has 118 edges initially, 4 edges after simplification

total ucounts = 14
nonsolo ucounts = 11[2^5, 3^2, 4, 8, 40, 64]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.26 = CGAATGTGTCTAGTGT-1

using 115 reads

====================================================================================

graph has 74 edges initially, 16 edges after simplification

total ucounts = 21
nonsolo ucounts = 12[2^6, 4, 12, 18, 19, 20, 21]
surviving nonsolo ucounts = 1[19]
ids = [19]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.29 = CGAATGTGTGAAGGCT-1

using 6246 reads

====================================================================================

graph has 3500 edges initially, 88 edges after simplification

total ucounts = 396
nonsolo ucounts = 277[2^46, 3^32, 4^20, 5^24, 6^22, 7^14, 8^10, 9^6, 10^6, 11^12, 12^14, 13^6, 14^3, 15^8, 16^5, 17^5, 18^3, 19^3, 20^2, 21^2, 22^3, 23^3, 24^2, 26, 27, 28^2, 29^2, 30^3, 31, 32^3, 33, 34, 37^2, 41^3, 45, 81, 82, 89, 140, 3149]
surviving nonsolo ucounts = 2[140, 3149]
ids = [285, 142]

====================================================================================

REJECT CONTIGS

TIG 1[bases=376]
19-123 ==> 0-104 on segment before TRBJ2-3 exon 1 [len=104] (mis=0)
123-172 ==> 0-49 on |546|TRBJ2-3|J-REGION| [len=49] (mis=0)
172-274 ==> 0-102 on segment before TRBJ2-4 exon 1 [len=102] (mis=0)
324-376 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
umis assigned: [142, 285]
of which 2 are surviving nonsolos
reads assigned: 3223
start codons at 33
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.31 = CGAATGTGTGCAGACA-1

using 11 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[11]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.33 = CGAATGTGTGGACGAT-1

using 934 reads

====================================================================================

graph has 1050 edges initially, 18 edges after simplification

total ucounts = 162
nonsolo ucounts = 103[2^21, 3^7, 4^15, 5^10, 6^7, 7^8, 8^5, 9^4, 11^2, 12^4, 13, 15^2, 16^2, 17^3, 19^2, 20^3, 22^2, 25, 37, 39, 42, 47]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.34 = CGAATGTGTGTAATGA-1

using 30 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.36 = CGAATGTGTGTGAATA-1

using 35411 reads

====================================================================================

graph has 8909 edges initially, 156 edges after simplification

total ucounts = 573
nonsolo ucounts = 413[2^76, 3^36, 4^24, 5^19, 6^21, 7^19, 8^21, 9^14, 10^16, 11^13, 12^11, 13^9, 14^10, 15^5, 16^11, 17^6, 18^4, 19^8, 20^7, 21^5, 22^10, 23^5, 24^5, 25^7, 26^3, 27^4, 28^3, 29, 30, 31^3, 32^2, 33^3, 34^2, 35^2, 36^4, 39^3, 40, 41^2, 43^3, 45, 49^2, 50, 71, 76^2, 91, 92, 103, 7206, 7513, 7633, 7814]
surviving nonsolo ucounts = 4[7206, 7513, 7633, 7814]
ids = [40, 279, 410, 56]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.44 = CGAATGTGTTCAGACT-1

using 273 reads

====================================================================================

graph has 198 edges initially, 8 edges after simplification

total ucounts = 11
nonsolo ucounts = 10[2, 3, 4^2, 31, 32, 38, 39, 59, 60]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.46 = CGAATGTGTTCTGAAC-1

using 15 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.47 = CGAATGTGTTCTGTTT-1

using 78 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[78]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.52 = CGAATGTTCAACGGCC-1

using 57 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[57]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.54 = CGAATGTTCAAGGTAA-1

using 89 reads

====================================================================================

graph has 132 edges initially, 2 edges after simplification

total ucounts = 14
nonsolo ucounts = 7[2, 3, 6, 10^2, 15, 36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.55 = CGAATGTTCACCTTAT-1

using 28 reads

====================================================================================

graph has 6 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.56 = CGAATGTTCACTTATC-1

using 34 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.59 = CGAATGTTCATATCGG-1

using 24623 reads

====================================================================================

graph has 10776 edges initially, 246 edges after simplification

total ucounts = 784
nonsolo ucounts = 577[2^90, 3^49, 4^47, 5^26, 6^32, 7^31, 8^18, 9^22, 10^18, 11^22, 12^11, 13^19, 14^16, 15^14, 16^13, 17^10, 18^10, 19^15, 20^9, 21^5, 22^10, 23^9, 24^5, 25^6, 26^6, 27^3, 28^4, 29^4, 30^7, 31^3, 32^4, 33, 34, 35, 36^2, 37, 38^2, 39^3, 40, 41^2, 46^4, 48^2, 49, 50, 53, 58^2, 59, 60, 98, 198, 275, 379, 441, 520, 661, 1108, 1347, 1868, 1988, 8988]
surviving nonsolo ucounts = 12[98, 198, 275, 379, 441, 520, 661, 1108, 1347, 1868, 1988, 8988]
ids = [323, 99, 98, 265, 101, 356, 52, 113, 90, 253, ...]

====================================================================================

UMI info for barcode CGAATGTTCATATCGG-1 contig 1 = GGGGAGATAT...
umi ACGTTTATGC = 661 reads: +391 validated
umi CCCCTCCGCA = 1778 reads: -363X +1 -1XX +2 -1XX +3 -1XX +1 -4XX +1 -2XX +1 -4XX +1 -2XX +2 -1XX invalidated
umi CCCTTTTTGT = 342 reads: +391 validated
umi CGTTGGATCG = 100 reads: +391 validated

UMI info for barcode CGAATGTTCATATCGG-1 contig 2 = CCCCGACGTG...
umi AGTCGTGGGC = 279 reads: -63 +337 non-validated
umi AGTCGTGGGG = 195 reads: -60 +1 -2X +337 invalidated
umi AGTCGTTGGC = 1997 reads: -60 +1 -2XX +337 invalidated
umi AGTCGTTGGG = 449 reads: -63 +337 non-validated
umi ATAGTTTGGG = 1107 reads: +400 validated
umi CAGCAACAGC = 9054 reads: +181 -10XX +2 -1XX +3 -1XX +1 -2XX +1 -2XX +1 -165XX +1 -4XX +1 -1XX +1 -8XX +1 -1XX +1 -2XX +1 -1XX +1 -2XX +1 -1XX +2 invalidated

GOOD CONTIGS

TIG 1[bases=481]
48-384 ==> 0-336 on |481|TRAV21|L-REGION+V-REGION| [len=336] (mis=4)
376-439 ==> 0-63 on |443|TRAJ57|J-REGION| [len=63] (mis=4)
439-481 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 2 umis using 113 reads
cdr3 = CAAQGGSEKLVF at 372, score = 9 + 9
umis assigned: [52, 253, 265, 323]
of which 4 are surviving nonsolos
reads assigned: 2856
start codons at 48, 92, 292
confident = true

TIG 2[bases=516]
0-64 ==> 17-81 on |585|TRBV18|5'UTR| [len=81] (mis=0)
64-411 ==> 0-347 on |586|TRBV18|L-REGION+V-REGION| [len=347] (mis=2)
408-420 ==> 0-12 on |535|TRBD1|D-REGION| [len=12] (mis=2)
424-464 ==> 8-48 on |537|TRBJ1-2|J-REGION| [len=48] (mis=0)
464-516 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 5 umis using 391 reads
cdr3 = CASSPRQGARGYTF at 394, score = 7 + 9
umis assigned: [98, 99, 100, 101, 113, 185]
of which 6 are surviving nonsolos
reads assigned: 12816
start codons at 49, 64, 122, 133, 186, 196, 212, 256, 287, 298
confident = true

REJECT CONTIGS

TIG 1[bases=591]
0-46 ==> 0-46 on |661|TRBV6-6|5'UTR| [len=46] (mis=0)
46-91 ==> 0-45 on |662|TRBV6-6|L-REGION+V-REGION| [len=344] (mis=0)
49-148 ==> 0-99 on segment before TRBV6-7 exon 2 [len=135] (mis=13)
95-184 ==> 0-89 on segment before TRBV6-6 exon 2 [len=89] (mis=2)
180-479 ==> 45-344 on |662|TRBV6-6|L-REGION+V-REGION| [len=344] (mis=3) [SHIFT!]
476-492 ==> 0-16 on |730|TRBD2|D-REGION| [len=16] (mis=2)
497-539 ==> 8-50 on |543|TRBJ2-1|J-REGION| [len=50] (mis=0)
539-591 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
umis assigned: [90, 356]
of which 2 are surviving nonsolos
reads assigned: 1824
start codons at 4, 46, 193, 246, 270, 285, 312, 376, 497
confident = false
did not find CDR3
now this is a cell
paired!

GCTTCTCAGCCTGGTGACTCAGCCACCTACCTCTGTGCTGCGCAGGGCGGATCTGAAAAGCTGGTCTTTGGAAAGGGAACGAAACTGACAGTAAACCCAT <==> GTAGTGCGAGGAGATTCGGCAGCTTATTTCTGTGCCAGCTCACCTCGACAGGGGGCGCGGGGCTACACCTTCGGTTCGGGGACCAGGTTAACCGTTGTAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.60 = CGAATGTTCCAAATGC-1

using 38 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.62 = CGAATGTTCCGCAGTG-1

using 88 reads

====================================================================================

graph has 62 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[88]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.68 = CGAATGTTCGCAAACT-1

using 1404 reads

====================================================================================

graph has 1030 edges initially, 38 edges after simplification

total ucounts = 65
nonsolo ucounts = 52[2^6, 3^3, 4, 5^3, 7, 8^5, 9^2, 10^4, 11, 14, 15, 16, 17, 18^3, 22, 29^2, 30, 34, 41, 43, 46, 51, 52, 58, 72, 75, 76, 78, 80, 96, 102, 105]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.69 = CGAATGTTCGCAGGCT-1

using 3204 reads

====================================================================================

graph has 1126 edges initially, 10 edges after simplification

total ucounts = 9
nonsolo ucounts = 7[2, 3, 13, 34, 774, 1086, 1290]
surviving nonsolo ucounts = 3[774, 1086, 1290]
ids = [4, 0, 7]

====================================================================================

UMI info for barcode CGAATGTTCGCAGGCT-1 contig 1 = AGATGCTTCA...
umi AAACCGATGG = 1084 reads: +403 validated
umi CGGCATTTCC = 1213 reads: -388X +2 -11XX +2 invalidated

UMI info for barcode CGAATGTTCGCAGGCT-1 contig 2 = GAGCACTCTT...
umi CAATGTCTCG = 775 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=491]
0-36 ==> 41-77 on |578|TRBV15|5'UTR| [len=77] (mis=0)
36-372 ==> 0-336 on |579|TRBV15|L-REGION+V-REGION| [len=344] (mis=0)
375-391 ==> 0-16 on |730|TRBD2|D-REGION| [len=16] (mis=3)
389-439 ==> 0-50 on |543|TRBJ2-1|J-REGION| [len=50] (mis=1)
439-491 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 1 umis using 75 reads
cdr3 = CATGGTSGIPYNEQFF at 363, score = 6 + 8
umis assigned: [0, 7]
of which 2 are surviving nonsolos
reads assigned: 2264
start codons at 2, 17, 36, 60, 88, 94, 99, 186, 241, 259, 354, 397
confident = false

TIG 2[bases=533]
0-97 ==> 0-97 on |492|TRAV27|5'UTR| [len=97] (mis=1)
97-425 ==> 0-328 on |493|TRAV27|L-REGION+V-REGION| [len=328] (mis=2)
425-491 ==> 0-66 on |413|TRAJ28|J-REGION| [len=66] (mis=3)
491-533 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 1 umis using 38 reads
cdr3 = CAGEDSGAGSYQLTF at 415, score = 10 + 10
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 762
start codons at 70, 79, 97, 141, 249, 350
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.72 = CGAATGTTCTAACGGT-1

using 72 reads

====================================================================================

graph has 109 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[3, 6, 61]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.73 = CGAATGTTCTAGAGTC-1

using 5660 reads

====================================================================================

graph has 4541 edges initially, 166 edges after simplification

total ucounts = 565
nonsolo ucounts = 387[2^69, 3^51, 4^26, 5^14, 6^20, 7^23, 8^14, 9^11, 10^7, 11^6, 12^12, 13^12, 14^15, 15^9, 16^13, 17^6, 18^6, 19^6, 20^10, 21^6, 22^6, 23^5, 24^5, 25^5, 26^4, 27^4, 28, 29, 30, 31^2, 32, 33, 34, 35^3, 36^2, 37, 40, 49, 50, 56, 60, 63, 69, 1333]
surviving nonsolo ucounts = 1[1333]
ids = [556]

====================================================================================

REJECT CONTIGS

TIG 1[bases=562]
3-40 ==> 49-86 on segment before TRBJ2-2P exon 1 [len=86] (mis=0)
40-86 ==> 0-46 on |545|TRBJ2-2P|J-REGION| [len=46] (mis=0)
86-190 ==> 0-104 on segment before TRBJ2-3 exon 1 [len=104] (mis=0)
239-341 ==> 0-102 on segment before TRBJ2-4 exon 1 [len=102] (mis=0)
391-462 ==> 0-71 on segment before TRBJ2-5 exon 1 [len=71] (mis=0)
510-562 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
umis assigned: [556]
of which 1 are surviving nonsolos
reads assigned: 1317
start codons at 100
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.77 = CGAATGTTCTGTCTCG-1

using 61 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[61]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.78 = CGAATGTTCTGTTTGT-1

using 100 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 1[98]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.81 = CGAATGTTCTTGTACT-1

using 2367 reads

====================================================================================

graph has 2306 edges initially, 82 edges after simplification

total ucounts = 285
nonsolo ucounts = 202[2^34, 3^14, 4^18, 5^16, 6^12, 7^13, 8^6, 9^3, 10^9, 11^7, 12^5, 13^2, 14^5, 15^3, 16^3, 17^4, 18^6, 19^4, 20, 21, 22, 23^8, 24^4, 25^8, 26^2, 27^2, 28^2, 29, 32, 35^2, 40, 50, 53, 64, 73]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.83 = CGACCTTAGAACAATC-1

using 34 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.86 = CGACCTTAGACTGTAA-1

using 709 reads

====================================================================================

graph has 268 edges initially, 2 edges after simplification

total ucounts = 15
nonsolo ucounts = 7[2, 4, 5^2, 8^2, 669]
surviving nonsolo ucounts = 1[669]
ids = [11]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.88 = CGACCTTAGATAGGAG-1

using 82 reads

====================================================================================

graph has 90 edges initially, 2 edges after simplification

total ucounts = 12
nonsolo ucounts = 9[3^2, 5^2, 6, 12, 13, 14, 18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.89 = CGACCTTAGATATGGT-1

using 15 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.92 = CGACCTTAGCCAGGAT-1

using 79 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[79]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.96 = CGACCTTAGCGCCTTG-1

using 88 reads

====================================================================================

graph has 90 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[9, 77]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.97 = CGACCTTAGCGTAATA-1

using 30 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.99 = CGACCTTAGCTTTGGT-1

using 43 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 4[3, 4, 6, 26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.105 = CGACCTTAGGTGCACA-1

using 67 reads

====================================================================================

graph has 112 edges initially, 2 edges after simplification

total ucounts = 16
nonsolo ucounts = 9[2^4, 3^3, 10, 33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.106 = CGACCTTAGTACACCT-1

using 16 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[16]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.107 = CGACCTTAGTACCGGA-1

using 101 reads

====================================================================================

graph has 86 edges initially, 4 edges after simplification

total ucounts = 13
nonsolo ucounts = 10[2^3, 4, 7, 8, 10, 17, 19, 27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.110 = CGACCTTAGTCCAGGA-1

using 109 reads

====================================================================================

graph has 72 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 3[13, 16, 74]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.113 = CGACCTTAGTGTACCT-1

using 38 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 5[2, 3, 7, 8, 15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.116 = CGACCTTCAAACTGTC-1

using 23 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.117 = CGACCTTCAAAGCAAT-1

using 52 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 7[2, 4, 5, 8, 9^2, 14]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.118 = CGACCTTCAAATCCGT-1

using 52 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[5, 45]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.120 = CGACCTTCAAGCCATT-1

using 21 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.122 = CGACCTTCAAGGTTCT-1

using 335 reads

====================================================================================

graph has 458 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[5, 329]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.125 = CGACCTTCAATAGCGG-1

using 171 reads

====================================================================================

graph has 82 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[171]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.126 = CGACCTTCAATCACAC-1

using 196 reads

====================================================================================

graph has 136 edges initially, 6 edges after simplification

total ucounts = 14
nonsolo ucounts = 11[2^2, 3^2, 5, 13, 18, 34, 35, 39^2]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.130 = CGACCTTCACACATGT-1

using 74 reads

====================================================================================

graph has 66 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 5[2, 6, 7, 8, 49]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.134 = CGACCTTCACCCATGG-1

using 3218 reads

====================================================================================

graph has 774 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 3[4, 62, 3147]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.137 = CGACCTTCACGAAATA-1

using 14 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[14]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.139 = CGACCTTCACGGTGTC-1

using 77 reads

====================================================================================

graph has 60 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 8[2, 3, 4, 5, 7, 14, 19, 20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.141 = CGACCTTCAGACAAAT-1

using 49 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[49]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.146 = CGACCTTCAGATCTGT-1

using 1355 reads

====================================================================================

graph has 1140 edges initially, 10 edges after simplification

total ucounts = 18
nonsolo ucounts = 16[2^2, 4^2, 8, 9, 18, 30, 42, 52, 60, 81, 98, 277, 295, 371]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.147 = CGACCTTCAGATGAGC-1

using 44 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 2[6, 35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.151 = CGACCTTCAGCTGCTG-1

using 50 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[50]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.152 = CGACCTTCAGCTTAAC-1

using 7618 reads

====================================================================================

graph has 6990 edges initially, 200 edges after simplification

total ucounts = 1056
nonsolo ucounts = 750[2^135, 3^94, 4^55, 5^55, 6^47, 7^44, 8^28, 9^28, 10^24, 11^21, 12^23, 13^24, 14^21, 15^21, 16^17, 17^8, 18^10, 19^5, 20^6, 21^8, 22^10, 23^8, 24^3, 25^6, 26^7, 27^7, 28, 29^2, 30, 31^3, 32^3, 34, 35^3, 36, 37^2, 41, 42^2, 43, 44, 45^2, 46, 47, 48, 50, 51, 53, 54, 56, 59, 115, 127]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.160 = CGACCTTCATAACCTG-1

using 110 reads

====================================================================================

graph has 48 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[109]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.161 = CGACCTTCATACGCCG-1

using 72 reads

====================================================================================

graph has 44 edges initially, 4 edges after simplification

total ucounts = 3
nonsolo ucounts = 3[5, 21, 46]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.162 = CGACCTTCATAGTAAG-1

using 13443 reads

====================================================================================

graph has 8423 edges initially, 264 edges after simplification

total ucounts = 1147
nonsolo ucounts = 826[2^131, 3^110, 4^69, 5^65, 6^39, 7^44, 8^33, 9^34, 10^31, 11^20, 12^26, 13^20, 14^22, 15^18, 16^10, 17^10, 18^13, 19^11, 20^12, 21^8, 22^6, 23^9, 24^7, 25^2, 26^4, 27^13, 28^6, 29^4, 30^3, 31^2, 32^2, 33^5, 34^3, 35, 36^2, 37^2, 38^2, 39^4, 42^2, 44^2, 46, 49, 50, 54, 55^2, 62, 63^2, 65, 74, 75^2, 100, 106, 456, 661, 1503, 1979]
surviving nonsolo ucounts = 8[2^2, 3, 50, 456, 661, 1503, 1979]
ids = [498, 940, 580, 888, 390, 564, 121, 329]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.163 = CGACCTTCATCCCACT-1

using 48 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[48]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.164 = CGACCTTCATCCGGGT-1

using 78 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[78]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.166 = CGACCTTCATCCTAGA-1

using 43 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[43]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.167 = CGACCTTCATCTATGG-1

using 21 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.169 = CGACCTTCATGGTAGG-1

using 438 reads

====================================================================================

graph has 372 edges initially, 28 edges after simplification

total ucounts = 27
nonsolo ucounts = 23[2^3, 3^2, 4^2, 5^3, 7, 8, 13, 14, 18, 20, 26, 33, 35, 42, 45, 53, 85]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.173 = CGACCTTCATGTTGAC-1

using 21 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.175 = CGACCTTCATTCTCAT-1

using 107 reads

====================================================================================

graph has 70 edges initially, 4 edges after simplification

total ucounts = 11
nonsolo ucounts = 6[2, 5, 7, 8, 20, 60]
surviving nonsolo ucounts = 1[60]
ids = [8]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.178 = CGACCTTGTAAACCTC-1

using 50 reads

====================================================================================

graph has 54 edges initially, 2 edges after simplification

total ucounts = 13
nonsolo ucounts = 5[2^2, 3, 4, 31]
surviving nonsolo ucounts = 1[31]
ids = [11]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.183 = CGACCTTGTACCGTAT-1

using 175 reads

====================================================================================

graph has 84 edges initially, 4 edges after simplification

total ucounts = 8
nonsolo ucounts = 6[2, 6, 7, 26^2, 106]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.185 = CGACCTTGTAGGACAC-1

using 34 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.195 = CGACCTTGTCCATCCT-1

using 61 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[61]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.197 = CGACCTTGTCCCTACT-1

using 26 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.199 = CGACCTTGTCCTCCAT-1

using 23 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.203 = CGACCTTGTCTAGTCA-1

using 121 reads

====================================================================================

graph has 122 edges initially, 6 edges after simplification

total ucounts = 23
nonsolo ucounts = 12[2^3, 3, 5^2, 9, 10, 16, 18, 19^2]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.206 = CGACCTTGTCTCTCTG-1

using 18 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.208 = CGACCTTGTCTGATCA-1

using 73 reads

====================================================================================

graph has 52 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 6[4^2, 6, 7, 12, 37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.211 = CGACCTTGTCTTGCGG-1

using 112 reads

====================================================================================

graph has 64 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 1[107]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.215 = CGACCTTGTGCACCAC-1

using 75 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[3, 72]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.217 = CGACCTTGTGCCTGCA-1

using 111 reads

====================================================================================

graph has 56 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[111]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.218 = CGACCTTGTGCGATAG-1

using 58 reads

====================================================================================

graph has 60 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 5[2^2, 4, 18, 29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.223 = CGACCTTGTGGTCTCG-1

using 48 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[48]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.226 = CGACCTTGTGTTGAGG-1

using 311 reads

====================================================================================

graph has 182 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[7, 304]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.228 = CGACCTTGTTACGACT-1

using 680 reads

====================================================================================

graph has 206 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[680]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.237 = CGACCTTGTTGGTAAA-1

using 23 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.239 = CGACCTTGTTGTCGCG-1

using 86 reads

====================================================================================

graph has 90 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 9[2^2, 6, 7, 8^2, 10, 21^2]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.242 = CGACCTTTCAACACAC-1

using 116 reads

====================================================================================

graph has 106 edges initially, 6 edges after simplification

total ucounts = 13
nonsolo ucounts = 11[2^2, 5^2, 7^2, 8, 13, 17, 23, 25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.244 = CGACCTTTCAACGCTA-1

using 64 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[64]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.245 = CGACCTTTCAAGATCC-1

using 35 reads

====================================================================================

graph has 6 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.246 = CGACCTTTCAAGGTAA-1

using 116 reads

====================================================================================

graph has 74 edges initially, 4 edges after simplification

total ucounts = 12
nonsolo ucounts = 7[2^2, 3^2, 4, 47, 50]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.248 = CGACCTTTCACGGTTA-1

using 74 reads

====================================================================================

graph has 68 edges initially, 6 edges after simplification

total ucounts = 17
nonsolo ucounts = 9[2^3, 3^3, 10, 17, 24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.254 = CGACCTTTCATCTGCC-1

using 74 reads

====================================================================================

graph has 92 edges initially, 4 edges after simplification

total ucounts = 10
nonsolo ucounts = 8[3^2, 5, 9, 10, 13, 14, 15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.255 = CGACCTTTCATCTGTT-1

using 457 reads

====================================================================================

graph has 294 edges initially, 12 edges after simplification

total ucounts = 23
nonsolo ucounts = 21[2^4, 3^2, 4^3, 5, 6, 9, 14, 19, 22, 23, 48, 52, 64, 76, 91]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.256 = CGACCTTTCATGCATG-1

using 70 reads

====================================================================================

graph has 74 edges initially, 2 edges after simplification

total ucounts = 17
nonsolo ucounts = 11[2^3, 3, 4^3, 6^2, 11, 20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.258 = CGACCTTTCATGGTCA-1

using 111 reads

====================================================================================

graph has 68 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[110]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.259 = CGACCTTTCATGTCTT-1

using 143 reads

====================================================================================

graph has 48 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 3[2, 10, 131]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.261 = CGACCTTTCCAACCAA-1

using 71 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[71]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.262 = CGACCTTTCCAAGTAC-1

using 35 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.263 = CGACCTTTCCACGAAT-1

using 80000 reads

====================================================================================

graph has 33566 edges initially, 756 edges after simplification

total ucounts = 3839
nonsolo ucounts = 2860[2^444, 3^355, 4^290, 5^198, 6^173, 7^144, 8^144, 9^129, 10^87, 11^104, 12^88, 13^93, 14^63, 15^51, 16^49, 17^43, 18^44, 19^32, 20^31, 21^41, 22^18, 23^23, 24^18, 25^17, 26^10, 27^18, 28^16, 29^10, 30^12, 31^7, 32^7, 33^8, 34^4, 35^5, 36^4, 37^4, 38^4, 39, 40^2, 41^3, 42^6, 43, 44^2, 45^3, 46^4, 47^3, 48^3, 49, 50^3, 52^2, 54^2, 57^2, 60, 65, 68, 69, 71^2, 73, 76^2, 77^2, 78, 79, 82, 83, 86, 91^2, 94, 95, 97, 118, 965, 1215, 1448, 3182, 3582, 4161, 4179, 4656, 6117, 6945, 7487, 7681]
surviving nonsolo ucounts = 12[965, 1215, 1448, 3182, 3582, 4161, 4179, 4656, 6117, 6945, 7487, 7681]
ids = [3466, 504, 1557, 2286, 556, 3819, 491, 3122, 2517, 492, ...]

====================================================================================

REJECT CONTIGS

TIG 1[bases=335]
5-236 ==> 647-878 on segment before TRAJ33 exon 1 [len=878] (mis=0)
236-293 ==> 0-57 on |419|TRAJ33|J-REGION| [len=57] (mis=0)
293-335 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
umis assigned: [491, 504, 1238, 1557, 3466]
of which 5 are surviving nonsolos
reads assigned: 15166
start codons at 16, 29, 56, 81, 91, 124, 142
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.272 = CGACCTTTCCTTCAAT-1

using 20 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.275 = CGACCTTTCGACCAGC-1

using 1782 reads

====================================================================================

graph has 604 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 4[2, 3, 6, 1764]
surviving nonsolo ucounts = 1[1764]
ids = [1]

====================================================================================

UMI info for barcode CGACCTTTCGACCAGC-1 contig 1 = AGACCAGAAT...
umi ATGACGTCGC = 1770 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=491]
0-47 ==> 0-47 on |616|TRBV3-1|5'UTR| [len=47] (mis=0)
47-391 ==> 0-344 on |617|TRBV3-1|L-REGION+V-REGION| [len=344] (mis=0)
394-441 ==> 0-47 on |550|TRBJ2-7|J-REGION| [len=47] (mis=5)
439-491 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 1 umis using 125 reads
cdr3 = CASSQDTQSEQYF at 374, score = 9 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 1738
start codons at 47, 146, 169, 189, 197, 239, 273
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.278 = CGACCTTTCGCAAGCC-1

using 1718 reads

====================================================================================

graph has 430 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 5[2, 3, 8, 21, 1680]
surviving nonsolo ucounts = 1[1680]
ids = [7]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.280 = CGACCTTTCGCCAAAT-1

using 35 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.284 = CGACCTTTCGCGTTTC-1

using 29 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.285 = CGACCTTTCGCTGATA-1

using 29 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.287 = CGACCTTTCGTACCGG-1

using 437 reads

====================================================================================

graph has 218 edges initially, 4 edges after simplification

total ucounts = 15
nonsolo ucounts = 12[2^5, 3^2, 5, 6, 10, 18, 379]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.289 = CGACCTTTCGTCACGG-1

using 67 reads

====================================================================================

graph has 60 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 4[2, 5, 20, 36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.292 = CGACCTTTCTAACTGG-1

using 42 reads

====================================================================================

graph has 40 edges initially, 4 edges after simplification

total ucounts = 11
nonsolo ucounts = 7[2, 3^2, 4, 5^2, 16]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.297 = CGACCTTTCTCGAGTA-1

using 30 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.299 = CGACCTTTCTCTTATG-1

using 72 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 7[2^2, 3^2, 6, 17, 39]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.300 = CGACCTTTCTCTTGAT-1

using 63 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[63]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.302 = CGACCTTTCTGCCAGG-1

using 35 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.304 = CGACCTTTCTGGCGAC-1

using 44 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 2[12, 29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.306 = CGACCTTTCTGTCTAT-1

using 35 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.307 = CGACCTTTCTTAACCT-1

using 92 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[92]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.308 = CGACCTTTCTTCAACT-1

using 50 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[50]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.310 = CGACCTTTCTTCTGGC-1

using 34 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 4[2, 3, 6, 19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.317 = CGACTTCAGACAGGCT-1

using 33 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.319 = CGACTTCAGAGTGACC-1

using 20813 reads

====================================================================================

graph has 7719 edges initially, 202 edges after simplification

total ucounts = 781
nonsolo ucounts = 560[2^96, 3^59, 4^51, 5^38, 6^33, 7^22, 8^24, 9^16, 10^20, 11^18, 12^7, 13^12, 14^14, 15^11, 16^8, 17^6, 18^6, 19^4, 20^11, 21^13, 22^4, 23^5, 24^4, 25^4, 26^4, 27^4, 29^3, 30^3, 31^2, 32, 33^5, 34^2, 35^4, 36^2, 37^3, 38^2, 39^2, 40, 41^3, 42, 43^2, 45^2, 46^2, 47^3, 48^4, 49, 50, 51, 53^2, 54, 64, 65, 66, 68, 92, 101, 103, 109, 127, 148, 3096, 3608, 6880]
surviving nonsolo ucounts = 4[148, 3096, 3608, 6880]
ids = [600, 307, 743, 84]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.323 = CGACTTCAGATGTGGC-1

using 35 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.325 = CGACTTCAGCCAGTAG-1

using 51 reads

====================================================================================

graph has 54 edges initially, 6 edges after simplification

total ucounts = 9
nonsolo ucounts = 8[2^2, 3, 4, 5, 6, 8, 20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.333 = CGACTTCAGCTCCCAG-1

using 86 reads

====================================================================================

graph has 48 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[85]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.334 = CGACTTCAGCTGGAAC-1

using 31 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.335 = CGACTTCAGCTGTTCA-1

using 51 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[51]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.338 = CGACTTCAGGACAGCT-1

using 46 reads

====================================================================================

graph has 52 edges initially, 2 edges after simplification

total ucounts = 14
nonsolo ucounts = 8[3^3, 4, 5^2, 8, 9]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.339 = CGACTTCAGGAGCGAG-1

using 80000 reads

====================================================================================

graph has 19352 edges initially, 214 edges after simplification

total ucounts = 1149
nonsolo ucounts = 819[2^137, 3^95, 4^71, 5^55, 6^57, 7^33, 8^37, 9^20, 10^25, 11^24, 12^7, 13^16, 14^17, 15^17, 16^7, 17^11, 18^13, 19^2, 20^9, 21^4, 22^5, 23^8, 24^9, 25^4, 26^3, 27, 28^3, 29^5, 30^4, 31^3, 32^3, 34^2, 35, 36^2, 37^3, 40^3, 41, 42^3, 43, 45, 48^2, 50, 53, 54, 58, 59, 60, 62, 68, 70, 77, 94, 104, 113, 139, 241, 274, 286, 293, 304, 317, 331, 337, 362, 415, 427, 439, 448, 456, 464, 481, 482, 504^2, 531, 573, 646, 659, 663, 682, 702, 718, 731, 745, 748, 755, 771, 772, 786, 799, 807, 820, 837, 843, 855, 863, 866, 877, 885, 906, 907, 916, 934, 936^2, 946, 950, 960^2, 965, 992, 1034, 1042, 1047, 1072, 1083, 1087, 1136, 1158, 1163, 1166, 1171, 1184, 1186, 1211, 1239, 1246, 1264, 1333, 1355, 1404, 1430, 1464, 1618, 1656, 4942]
surviving nonsolo ucounts = 80[274, 286, 293, 304, 317, 331, 337, 362, 415, 427, 439, 448, 456, 464, 481, 482, 504^2, 531, 573, 646, 659, 663, 682, 702, 718, 731, 745, 748, 755, 771, 772, 786, 799, 807, 820, 837, 843, 855, 863, 866, 877, 885, 906, 907, 916, 934, 936^2, 946, 950, 960^2, 965, 992, 1034, 1042, 1047, 1072, 1083, 1087, 1136, 1158, 1163, 1166, 1171, 1184, 1186, 1211, 1239, 1246, 1264, 1333, 1355, 1404, 1430, 1464, 1618, 1656, 4942]
ids = [476, 605, 920, 831, 1101, 520, 907, 640, 565, 817, ...]

====================================================================================

UMI info for barcode CGACTTCAGGAGCGAG-1 contig 1 = ACAGTGATCC...
umi AAAATTGTGT = 1087 reads: +397 validated
umi AACGCACCCT = 906 reads: +397 validated
umi AACTACGCGG = 1041 reads: +397 validated
umi AATGGTCGCG = 1186 reads: +162 -1XX +2 -1XX +81 -5XX +145 invalidated
umi ACACTCTTAT = 1400 reads: +397 validated
umi ACCCTGTATC = 1249 reads: +397 validated
umi ACCCTTACGA = 1053 reads: +397 validated
umi ACGGTTGGCT = 1132 reads: +397 validated
umi ACGGTTTAGT = 1069 reads: +397 validated
umi ACTGCGGGAA = 666 reads: +380 -1 +16 non-validated
umi AGCAAAAGGT = 456 reads: +397 validated
umi AGCACGGTGC = 982 reads: +391 -6XX invalidated
umi AGCCGCCTCA = 1008 reads: +397 validated
umi AGCGCATCAG = 1192 reads: +397 validated
umi AGGCCTGTAT = 1158 reads: +397 validated
umi AGGTACCTGG = 1215 reads: +397 validated
umi AGTCACCTAT = 995 reads: +273 -1XX +123 invalidated
umi AGTGTCGTGA = 1008 reads: +103 -1XX +293 invalidated
umi ATAAACCTAA = 649 reads: +397 validated
umi ATCCTGTTTG = 1262 reads: +397 validated
umi ATGTTGTCAG = 947 reads: +397 validated
umi ATTCCAGGCG = 1335 reads: +397 validated
umi ATTGGCACAA = 452 reads: +397 validated
umi ATTGTTCCGC = 1041 reads: +397 validated
umi ATTTTCTCAT = 734 reads: +397 validated
umi ATTTTTCACT = 861 reads: +397 validated
umi CAAGATTCGG = 737 reads: +397 validated
umi CACCATGAAA = 901 reads: +397 validated
umi CACCCGCTCA = 1255 reads: +397 validated
umi CAGAGGTTTC = 732 reads: +397 validated
umi CATAATCAGC = 498 reads: +397 validated
umi CCCTTTAGAG = 1365 reads: +397 validated
umi CCGGCCTGGG = 805 reads: +397 validated
umi CCTAACAGTA = 579 reads: +397 validated
umi CCTGGGATAG = 256 reads: +334 -57 +6 non-validated
umi CGCCCGGCCC = 664 reads: +397 validated
umi CGTCACACGT = 331 reads: +397 validated
umi CGTTAATGCG = 1178 reads: +397 validated
umi CGTTCTATAC = 956 reads: +397 validated
umi CTATAAGTAT = 990 reads: +397 validated
umi CTCTTTATTA = 851 reads: +397 validated
umi CTGTGCTGTA = 733 reads: +397 validated
umi CTTAGCTATT = 410 reads: +397 validated
umi GAAGGGGTGA = 881 reads: +397 validated
umi GACTCCCGGG = 286 reads: +397 validated
umi GATCACAATC = 727 reads: +397 validated
umi GATCACCAAC = 1416 reads: -268 +129 non-validated
umi GCGAAACGCA = 485 reads: +397 validated
umi GCGCAACCAC = 711 reads: +397 validated
umi GCGGATCTTG = 535 reads: +397 validated
umi GCGTTATGCA = 1 reads: -396X +1 invalidated
umi GTCTATTCTT = 933 reads: +397 validated
umi GTGCCCGCAG = 1391 reads: -319X +1 -1XX +1 -2XX +1 -1XX +1 -6XX +1 -4XX +1 -1XX +1 -3XX +1 -1XX +1 -2XX +1 -3XX +42 -1XX +1 invalidated
umi GTGTCTCCTA = 767 reads: +397 validated
umi GTTATAATCG = 843 reads: +397 validated
umi TAAGTGGGTG = 427 reads: +397 validated
umi TAATAATTTC = 772 reads: +397 validated
umi TACATAGGTC = 671 reads: +397 validated
umi TACCCGTAGG = 840 reads: +397 validated
umi TACCGCATAT = 793 reads: +397 validated
umi TATCAACTAG = 1174 reads: +397 validated
umi TCACATATAC = 460 reads: +373 -1 +2 -1 +20 non-validated
umi TCACTTTGCT = 833 reads: +397 validated
umi TCAGAGGAGT = 339 reads: +397 validated
umi TCCACCGTTG = 1462 reads: +397 validated
umi TCGATTCCGA = 814 reads: +397 validated
umi TCTACGCCGG = 1662 reads: +397 validated
umi TGACTCAGGG = 883 reads: +397 validated
umi TTAGCTAGCG = 1140 reads: +397 validated
umi TTCCAACTTT = 1194 reads: +397 validated
umi TTCCCTGATC = 929 reads: +397 validated
umi TTGGACTCAA = 901 reads: +397 validated
umi TTTCATTGGC = 819 reads: +397 validated

UMI info for barcode CGACTTCAGGAGCGAG-1 contig 2 = GTAGCTCGTT...
umi AAACGTTAAT = 466 reads: +358 -11 +19 non-validated
umi ACGATTGCGG = 441 reads: +329 -42 +17 non-validated
umi CTCATTAGTC = 3795 reads: -368X +1 -2XX +1 -1XX +2 -1XX +1 -2XX +1 -1XX +1 -5XX +1 invalidated
umi GCATGACCGA = 367 reads: +388 validated
umi GTATAGCGAT = 480 reads: +355 -1 +32 non-validated
umi TACCCGGAAT = 301 reads: +309 -1X +31 -33 +14 invalidated
umi TCCATCAGGA = 294 reads: +333 -1X +8 -29 +17 invalidated
umi TTGCCGCCTC = 332 reads: +348 -23 +1 -1 +15 non-validated

GOOD CONTIGS

TIG 1[bases=496]
0-47 ==> 0-47 on |674|TRBV7-2|5'UTR| [len=47] (mis=0)
47-394 ==> 0-347 on |675|TRBV7-2|L-REGION+V-REGION| [len=347] (mis=6)
396-444 ==> 0-48 on |548|TRBJ2-5|J-REGION| [len=48] (mis=3)
444-496 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 69 umis using 3887 reads
cdr3 = CASSPWKRETQYF at 377, score = 8 + 7
umis assigned: [0, 26, 31, 63, 76, 91, 93, 127, 128, 153] and 63 others
of which 72 are surviving nonsolos
reads assigned: 63115
start codons at 47, 156
confident = true

TIG 2[bases=650]
0-220 ==> 0-220 on |525|TRAV8-6|5'UTR| [len=220] (mis=2)
220-561 ==> 0-341 on |526|TRAV8-6|L-REGION+V-REGION| [len=341] (mis=3)
565-608 ==> 14-57 on |417|TRAJ31|J-REGION| [len=57] (mis=0)
608-650 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 2 umis using 16 reads
cdr3 = CAVSGERLMF at 547, score = 7 + 7
umis assigned: [5, 108, 546, 640, 738, 831, 920, 1101]
of which 8 are surviving nonsolos
reads assigned: 6399
start codons at 220, 383, 571, 581
confident = true
now this is a cell
paired!

AAACCCTCAGTCCATATAAGCGACACGGCTGAGTACTTCTGTGCTGTGAGTGGGGAAAGACTCATGTTTGGAGATGGAACTCAGCTGGTGGTGAAGCCCA <==> CGCACACAGCAGGAGGACTCAGCCGTGTATCTCTGTGCCAGCAGCCCCTGGAAGAGAGAGACCCAGTACTTCGGGCCAGGCACGCGGCTCCTGGTGCTCG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.341 = CGACTTCAGGAGTCTG-1

using 77 reads

====================================================================================

graph has 82 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 5[4, 6, 10, 12, 45]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.342 = CGACTTCAGGATATAC-1

using 17 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[17]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.351 = CGACTTCAGGTGTTAA-1

using 170 reads

====================================================================================

graph has 80 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[18, 152]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.353 = CGACTTCAGTAGCCGA-1

using 49 reads

====================================================================================

graph has 64 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 4[4^2, 14, 24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.356 = CGACTTCAGTCCCACG-1

using 44 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[44]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.358 = CGACTTCAGTCTCCTC-1

using 28 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.360 = CGACTTCAGTGCGATG-1

using 54 reads

====================================================================================

graph has 68 edges initially, 2 edges after simplification

total ucounts = 12
nonsolo ucounts = 8[2, 3^3, 4^2, 5, 26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.361 = CGACTTCAGTGGCACA-1

using 32 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.362 = CGACTTCAGTGTACCT-1

using 44 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[44]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.363 = CGACTTCAGTGTTGAA-1

using 773 reads

====================================================================================

graph has 613 edges initially, 20 edges after simplification

total ucounts = 42
nonsolo ucounts = 34[2^5, 3^3, 4^2, 5, 6^2, 7, 9, 10, 13^2, 15, 16, 17, 20, 21, 22, 25, 29, 47, 55, 61, 63, 64, 68, 71, 75]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.366 = CGACTTCAGTTGAGTA-1

using 30 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.369 = CGACTTCCAAAGCGGT-1

using 199 reads

====================================================================================

graph has 146 edges initially, 4 edges after simplification

total ucounts = 22
nonsolo ucounts = 19[2^3, 3^2, 4^4, 5, 7, 10^2, 12, 13, 15, 20, 36, 40]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.370 = CGACTTCCAACACCCG-1

using 776 reads

====================================================================================

graph has 520 edges initially, 24 edges after simplification

total ucounts = 35
nonsolo ucounts = 29[2^9, 3, 4, 5, 6, 11, 12, 14, 16, 17, 20, 24, 31, 41, 55, 64, 75, 76, 86^2, 106]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.375 = CGACTTCCAAGTTCTG-1

using 781 reads

====================================================================================

graph has 314 edges initially, 4 edges after simplification

total ucounts = 8
nonsolo ucounts = 3[6, 33, 737]
surviving nonsolo ucounts = 1[737]
ids = [5]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.376 = CGACTTCCAATCTGCA-1

using 120 reads

====================================================================================

graph has 108 edges initially, 4 edges after simplification

total ucounts = 13
nonsolo ucounts = 10[2, 4^2, 5^2, 6, 16, 22, 26, 27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.378 = CGACTTCCAATGGACG-1

using 39 reads

====================================================================================

graph has 48 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 7[2^2, 3, 4^2, 6, 17]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.380 = CGACTTCCACAAGCCC-1

using 26 reads

====================================================================================

graph has 4 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.381 = CGACTTCCACAGTCGC-1

using 66 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[66]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.385 = CGACTTCCACCGAAAG-1

using 106 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[106]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.387 = CGACTTCCACGCTTTC-1

using 76 reads

====================================================================================

graph has 62 edges initially, 4 edges after simplification

total ucounts = 6
nonsolo ucounts = 6[3, 4, 7, 8, 25, 29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.391 = CGACTTCCACGTGAGA-1

using 47607 reads

====================================================================================

graph has 23980 edges initially, 833 edges after simplification

total ucounts = 1329
nonsolo ucounts = 1138[2^85, 3^63, 4^44, 5^38, 6^35, 7^29, 8^27, 9^29, 10^22, 11^23, 12^25, 13^23, 14^28, 15^14, 16^22, 17^14, 18^14, 19^17, 20^13, 21^17, 22^16, 23^19, 24^19, 25^18, 26^13, 27^8, 28^7, 29^9, 30^7, 31^8, 32^15, 33^14, 34^10, 35^10, 36^4, 37^6, 38^9, 39^7, 40^8, 41^7, 42^13, 43^8, 44^4, 45^9, 46^9, 47^4, 48^6, 49^4, 50^7, 51^6, 52^9, 53^8, 54^5, 55^6, 56^5, 57^4, 58^8, 59^9, 60^5, 61^5, 62^5, 63^3, 64^7, 65^6, 66^2, 67^8, 68^5, 69^3, 70^7, 71^4, 72^3, 73^3, 74^5, 75^3, 77^3, 78^4, 79^2, 80^5, 81^2, 82^5, 83^3, 84^5, 85^7, 86^8, 87^4, 88^3, 89^2, 90^2, 91^4, 92^3, 93, 94^4, 95, 96^2, 97^4, 98^3, 99, 100^2, 101^2, 102^3, 103^4, 104, 105, 107^3, 108^3, 109^4, 110, 111^2, 112^2, 114^3, 118, 119, 120, 121, 122^2, 125, 126, 127^2, 128, 130^2, 131, 133^2, 135, 140^2, 141, 144, 151, 155, 156, 213, 303, 328, 357, 621, 870, 885, 1603, 1843, 1965, 2550]
surviving nonsolo ucounts = 15[18, 29, 56, 155, 213, 303, 328, 357, 621, 870, 885, 1603, 1843, 1965, 2550]
ids = [102, 200, 403, 1293, 639, 121, 292, 1263, 969, 1087, ...]

====================================================================================

UMI info for barcode CGACTTCCACGTGAGA-1 contig 1 = ACAGTGATCC...
umi AGGAGTCTAT = 1627 reads: +403 validated
umi AGGGCCTGGA = 2 reads: -403X invalidated
umi AGTCTAGTTT = 304 reads: +403 validated
umi CAAGATAGCG = 19 reads: -400X +3 invalidated
umi CAGCTTTTGT = 1 reads: -403X invalidated
umi CATTTGGACC = 2539 reads: +403 validated
umi CCAAACTCCG = 323 reads: +403 validated
umi CCTATTGGTA = 1832 reads: +403 validated
umi GATCTCTACT = 212 reads: +403 validated
umi GGTACATAGG = 2 reads: -400X +2 -1XX invalidated
umi TAGTGTAGGG = 622 reads: +403 validated
umi TCCCTATGTA = 1962 reads: +403 validated
umi TGCACAAACG = 1 reads: -403 non-validated
umi TGTGCACTCG = 3 reads: -400X +1 -2XX invalidated
umi TTCGTACCTT = 307 reads: -390X +2 -2XX +2 -1XX +1 -1XX +2 -1XX +1 invalidated
umi TTGGTTCGTA = 1 reads: -401X +2 invalidated
umi TTTAACATGG = 121 reads: -382 +1 -2X +2 -1X +1 -1X +2 -2XX +2 -1XX +1 -1XX +2 -1XX +1 invalidated

UMI info for barcode CGACTTCCACGTGAGA-1 contig 2 = GGGAAGAATA...
umi ACTGCTTGTT = 3 reads: -371 +1 -4XX +3 -2XX +1 -1XX +1 -1XX invalidated
umi AGGATTGCCG = 3 reads: -374X +1 -4XX +1 -5XX invalidated
umi ATACTTCTCT = 1 reads: -372X +1 -3X +3 -2X +2 -2X invalidated
umi ATTGGTGACT = 1 reads: -372X +1 -1X +1 -3X +2 -3X +1 -1X invalidated
umi CGCATAATTG = 3 reads: -370 +1 -3X +1 -4X +1 -5X invalidated
umi GGTACACTTC = 1 reads: -372X +1 -2X +1 -8X +1 invalidated
umi TCGTATTTTA = 876 reads: +385 validated
umi TTTAAGTTTC = 884 reads: +358 -8 +19 non-validated

GOOD CONTIGS

TIG 1[bases=502]
0-47 ==> 0-47 on |674|TRBV7-2|5'UTR| [len=47] (mis=0)
47-394 ==> 0-347 on |675|TRBV7-2|L-REGION+V-REGION| [len=347] (mis=2)
402-414 ==> 0-12 on |535|TRBD1|D-REGION| [len=12] (mis=2)
406-450 ==> 7-51 on |544|TRBJ2-2|J-REGION| [len=51] (mis=0)
450-502 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 8 umis using 607 reads
cdr3 = CASSLPAYRDGELFF at 377, score = 8 + 9
umis assigned: [100, 108, 121, 200, 247, 288, 292, 352, 639, 784] and 7 others
of which 11 are surviving nonsolos
reads assigned: 9712
start codons at 47, 156
confident = true

TIG 2[bases=556]
129-458 ==> 0-329 on |469|TRAV16|L-REGION+V-REGION| [len=329] (mis=0)
453-514 ==> 0-61 on |427|TRAJ40|J-REGION| [len=61] (mis=5)
514-556 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 1 umis using 32 reads
cdr3 = CALSGPGTYKYIF at 444, score = 8 + 8
umis assigned: [64, 102, 135, 178, 403, 783, 1087, 1294]
of which 4 are surviving nonsolos
reads assigned: 1744
start codons at 24, 63, 129, 292, 435
confident = true
now this is a cell
paired!

GCTCAAGAGGAAGACTCAGCCATGTATTACTGTGCTCTAAGTGGGCCAGGAACCTACAAATACATCTTTGGAACAGGCACCAGGCTGAAGGTTTTAGCAA <==> CAGCAGGAGGACTCGGCCGTGTATCTCTGTGCCAGCAGCCTACCAGCGTATCGGGACGGGGAGCTGTTTTTTGGAGAAGGCTCTAGGCTGACCGTACTGG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.394 = CGACTTCCACTGTCGG-1

using 39 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[39]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.395 = CGACTTCCACTTAACG-1

using 57 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[57]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.396 = CGACTTCCACTTAAGC-1

using 514 reads

====================================================================================

graph has 330 edges initially, 16 edges after simplification

total ucounts = 25
nonsolo ucounts = 16[2, 3, 4, 5^3, 6, 14, 28, 29, 40, 54, 69, 77, 81, 83]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.397 = CGACTTCCACTTCTGC-1

using 18 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.400 = CGACTTCCAGACACTT-1

using 47 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[10, 12, 24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.404 = CGACTTCCAGATGGCA-1

using 58 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[4, 8, 45]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.405 = CGACTTCCAGCTCGAC-1

using 93 reads

====================================================================================

graph has 96 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 9[2, 3^2, 5, 6^2, 7, 28, 31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.410 = CGACTTCCAGTCAGCC-1

using 132 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[132]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.412 = CGACTTCCAGTGAGTG-1

using 48 reads

====================================================================================

graph has 56 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 5[2, 4^2, 5, 31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.413 = CGACTTCCATACTCTT-1

using 72 reads

====================================================================================

graph has 80 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 3[3, 29, 37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.414 = CGACTTCCATCATCCC-1

using 35 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.416 = CGACTTCCATGACGGA-1

using 89 reads

====================================================================================

graph has 90 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 8[3^2, 8, 10^2, 12, 20, 22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.426 = CGACTTCCATTACCTT-1

using 46 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[4, 40]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.428 = CGACTTCCATTGAGCT-1

using 55 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[55]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.429 = CGACTTCGTAAACCTC-1

using 118 reads

====================================================================================

graph has 157 edges initially, 4 edges after simplification

total ucounts = 11
nonsolo ucounts = 9[2, 3, 4, 6, 8^2, 10, 26, 49]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.430 = CGACTTCGTAACGTTC-1

using 38 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.431 = CGACTTCGTAAGCACG-1

using 53 reads

====================================================================================

graph has 56 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[53]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.432 = CGACTTCGTACCGTAT-1

using 98 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[98]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.434 = CGACTTCGTAGCAAAT-1

using 129 reads

====================================================================================

graph has 98 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 5[4, 5, 30, 34, 54]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.435 = CGACTTCGTAGCCTAT-1

using 206 reads

====================================================================================

graph has 128 edges initially, 2 edges after simplification

total ucounts = 13
nonsolo ucounts = 10[2, 6, 7, 9^2, 11, 19, 43, 44, 53]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.437 = CGACTTCGTCAAACTC-1

using 32 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.439 = CGACTTCGTCAGTGGA-1

using 60 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 5[3^2, 4, 5, 44]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.440 = CGACTTCGTCGAAAGC-1

using 41 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[41]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.444 = CGACTTCGTCTTTCAT-1

using 93 reads

====================================================================================

graph has 48 edges initially, 4 edges after simplification

total ucounts = 6
nonsolo ucounts = 6[6, 15, 16^2, 17, 23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.446 = CGACTTCGTGCGGTAA-1

using 81 reads

====================================================================================

graph has 46 edges initially, 4 edges after simplification

total ucounts = 8
nonsolo ucounts = 4[2, 6, 13, 56]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.448 = CGACTTCGTGTGAATA-1

using 32 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[3, 8^2, 11]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.450 = CGACTTCGTTAAGAAC-1

using 110 reads

====================================================================================

graph has 80 edges initially, 4 edges after simplification

total ucounts = 6
nonsolo ucounts = 5[3, 10, 21, 35, 40]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.452 = CGACTTCGTTCAGACT-1

using 35 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.453 = CGACTTCGTTCCTCCA-1

using 37 reads

====================================================================================

graph has 6 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.455 = CGACTTCGTTGACGTT-1

using 39 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[39]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.457 = CGACTTCGTTGTGGAG-1

using 85 reads

====================================================================================

graph has 62 edges initially, 6 edges after simplification

total ucounts = 8
nonsolo ucounts = 6[4, 5^2, 9, 26, 34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.461 = CGACTTCTCAACGGGA-1

using 54 reads

====================================================================================

graph has 52 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[12, 42]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.462 = CGACTTCTCAAGGTAA-1

using 41 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[41]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.466 = CGACTTCTCACGCGGT-1

using 65 reads

====================================================================================

graph has 70 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[3, 19, 42]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.470 = CGACTTCTCACTTACT-1

using 45 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[45]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.471 = CGACTTCTCAGAAATG-1

using 56 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[56]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.472 = CGACTTCTCAGAGACG-1

using 10 reads

====================================================================================

graph has 6 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[10]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.476 = CGACTTCTCAGTGCAT-1

using 21 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.477 = CGACTTCTCAGTTCGA-1

using 213 reads

====================================================================================

graph has 156 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 9[2, 6^3, 8, 14, 23, 67, 80]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.480 = CGACTTCTCATGGTCA-1

using 81 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[81]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.481 = CGACTTCTCATGTCTT-1

using 172 reads

====================================================================================

graph has 78 edges initially, 4 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[76, 96]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.485 = CGACTTCTCCAATGGT-1

using 77 reads

====================================================================================

graph has 52 edges initially, 4 edges after simplification

total ucounts = 10
nonsolo ucounts = 5[2^2, 3, 32, 33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.486 = CGACTTCTCCAGAAGG-1

using 97 reads

====================================================================================

graph has 98 edges initially, 4 edges after simplification

total ucounts = 10
nonsolo ucounts = 4[2^2, 23, 64]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.489 = CGACTTCTCCCAACGG-1

using 10965 reads

====================================================================================

graph has 9083 edges initially, 266 edges after simplification

total ucounts = 1146
nonsolo ucounts = 824[2^118, 3^89, 4^81, 5^63, 6^45, 7^43, 8^51, 9^26, 10^32, 11^23, 12^20, 13^29, 14^22, 15^22, 16^21, 17^14, 18^15, 19^10, 20^11, 21^2, 22^5, 23^5, 24^4, 25^4, 26^6, 27, 28, 29^2, 30^2, 31^7, 32^2, 33^3, 34, 35^2, 36, 37^3, 38, 39, 40, 41^3, 43, 45, 46^3, 47, 50, 51, 52^3, 53, 54, 55^2, 56, 60, 61, 64^2, 69, 73, 75, 76, 91, 95^2, 100, 102, 104, 123, 1488]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.491 = CGACTTCTCCCGGATG-1

using 142 reads

====================================================================================

graph has 132 edges initially, 4 edges after simplification

total ucounts = 16
nonsolo ucounts = 12[2^2, 4, 5, 6, 7, 9, 11^2, 23, 29^2]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.492 = CGACTTCTCCGCATCT-1

using 80 reads

====================================================================================

graph has 48 edges initially, 4 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[23, 57]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.495 = CGACTTCTCCTCGCAT-1

using 47 reads

====================================================================================

graph has 44 edges initially, 4 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[7, 11, 27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.498 = CGACTTCTCCTGCTTG-1

using 21 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.499 = CGACTTCTCCTGTAGA-1

using 46 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 3[8, 16, 18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.504 = CGACTTCTCGCACTCT-1

using 137 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[136]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.508 = CGACTTCTCGGACAAG-1

using 36 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.510 = CGACTTCTCTATCCCG-1

using 59 reads

====================================================================================

graph has 4 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[59]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.515 = CGACTTCTCTCCGGTT-1

using 7823 reads

====================================================================================

graph has 6858 edges initially, 162 edges after simplification

total ucounts = 1081
nonsolo ucounts = 776[2^123, 3^96, 4^78, 5^56, 6^62, 7^41, 8^39, 9^37, 10^26, 11^27, 12^22, 13^25, 14^17, 15^11, 16^17, 17^8, 18^7, 19^9, 20^4, 21^2, 22^3, 23^6, 24^7, 25^3, 26^5, 27^4, 28^4, 29^4, 30, 31^2, 32^2, 33, 34^2, 36^3, 38^3, 39^2, 40^2, 41^2, 51^2, 54, 55, 59, 64^2, 68, 71, 77, 84, 106, 276]
surviving nonsolo ucounts = 2[106, 276]
ids = [976, 379]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.519 = CGACTTCTCTGGTTCC-1

using 143 reads

====================================================================================

graph has 74 edges initially, 6 edges after simplification

total ucounts = 6
nonsolo ucounts = 6[2^2, 4, 38, 41, 56]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.520 = CGACTTCTCTGTACGA-1

using 38 reads

====================================================================================

graph has 6 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.522 = CGACTTCTCTGTCTCG-1

using 54 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[54]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.523 = CGACTTCTCTTACCTA-1

using 102 reads

====================================================================================

graph has 58 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[102]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.531 = CGAGAAGAGAACAACT-1

using 13706 reads

====================================================================================

graph has 7932 edges initially, 207 edges after simplification

total ucounts = 562
nonsolo ucounts = 432[2^51, 3^30, 4^31, 5^26, 6^15, 7^19, 8^19, 9^15, 10^10, 11^12, 12^11, 13^6, 14^10, 15^12, 16^16, 17^9, 18^8, 19^14, 20^7, 21^10, 22^8, 23^13, 24^6, 25^7, 26^2, 27^7, 28^2, 29^2, 30^2, 31, 32^5, 33^3, 34^3, 35^2, 36^3, 37^2, 38^5, 39^4, 41, 42, 44, 45, 46^2, 47, 48, 51, 53, 54, 57, 59^2, 67, 526, 627, 678, 750, 867, 978, 1075, 1084, 1146]
surviving nonsolo ucounts = 9[526, 627, 678, 750, 867, 978, 1075, 1084, 1146]
ids = [378, 447, 310, 369, 475, 445, 398, 18, 276]

====================================================================================

UMI info for barcode CGAGAAGAGAACAACT-1 contig 1 = GAGAAGCCTC...
umi CTCGGACGAG = 1156 reads: +412 validated

UMI info for barcode CGAGAAGAGAACAACT-1 contig 2 = GTCCTGTCTC...
umi AACCTTTGCC = 1086 reads: +376 -10 +8 non-validated
umi GACAATCTTC = 677 reads: +368 -1 +11 -1 +13 non-validated
umi GGGAAAACCA = 741 reads: +331 -1 +4 -51 +7 non-validated
umi GTAACGGGGA = 522 reads: +371 -18 +5 non-validated
umi TAACAAGGTA = 1079 reads: +394 validated
umi TATTTCTGGA = 981 reads: +386 -2 +6 non-validated
umi TCACTGGCGA = 637 reads: +394 validated
umi TCTCTCTAGG = 863 reads: +365 -4 +1 -1 +1 -1X +1 -1 +1 -4 +1 -1 +12 invalidated

GOOD CONTIGS

TIG 1[bases=571]
0-117 ==> 0-117 on |474|TRAV19|5'UTR| [len=117] (mis=1)
117-467 ==> 0-350 on |475|TRAV19|L-REGION+V-REGION| [len=350] (mis=2)
470-529 ==> 0-59 on |421|TRAJ35|J-REGION| [len=59] (mis=1)
529-571 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 1 umis using 31 reads
cdr3 = CALSEGLRGFGNVLHC at 450, score = 7 + 6
umis assigned: [276]
of which 1 are surviving nonsolos
reads assigned: 1132
start codons at 111, 117, 174, 229, 250, 340, 349, 484
confident = true

TIG 2[bases=629]
0-183 ==> 25-208 on |589|TRBV19|5'UTR| [len=208] (mis=0)
183-527 ==> 0-344 on |590|TRBV19|L-REGION+V-REGION| [len=344] (mis=5)
523-535 ==> 0-12 on |535|TRBD1|D-REGION| [len=12] (mis=2)
534-577 ==> 7-50 on |543|TRBJ2-1|J-REGION| [len=50] (mis=0)
577-629 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 2 umis using 17 reads
cdr3 = CASTSDRGNEQFF at 510, score = 6 + 8
umis assigned: [18, 310, 369, 378, 398, 445, 447, 475]
of which 8 are surviving nonsolos
reads assigned: 6463
start codons at 183, 241, 292, 328, 333, 397, 535
confident = true
now this is a cell
paired!

GTGGACTCAGCAGTATACTTCTGTGCTCTGAGTGAGGGTTTGAGAGGCTTTGGGAATGTGCTGCATTGCGGGTCCGGCACTCAAGTGATTGTTTTACCAC <==> TCGGCCCAAAAGAACCCGACAGCTTTCTATCTCTGTGCCAGTACCTCGGACAGGGGGAATGAGCAGTTCTTCGGGCCAGGGACACGGCTCACCGTGCTAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.534 = CGAGAAGAGAGGTACC-1

using 41 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[41]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.536 = CGAGAAGAGAGTAAGG-1

using 54 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[54]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.538 = CGAGAAGAGATCTGAA-1

using 213 reads

====================================================================================

graph has 94 edges initially, 4 edges after simplification

total ucounts = 3
nonsolo ucounts = 3[2, 79, 132]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.540 = CGAGAAGAGATGTGGC-1

using 2544 reads

====================================================================================

graph has 560 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[2, 2540]
surviving nonsolo ucounts = 1[2540]
ids = [2]

====================================================================================

UMI info for barcode CGAGAAGAGATGTGGC-1 contig 1 = TGGGGAACAC...
umi CGTGTATAGG = 2543 reads: +385 validated

GOOD CONTIGS

TIG 1[bases=444]
17-355 ==> 0-338 on |530|TRAV9-2|L-REGION+V-REGION| [len=338] (mis=3)
351-402 ==> 9-60 on |441|TRAJ54|J-REGION| [len=60] (mis=0)
402-444 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 1 umis using 183 reads
cdr3 = CALRAQKLVF at 341, score = 9 + 9
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 2508
start codons at 17, 92, 180, 234
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.550 = CGAGAAGAGGCCGAAT-1

using 67 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[67]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.562 = CGAGAAGAGTGTGGCA-1

using 72 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[72]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.563 = CGAGAAGAGTTAGCGG-1

using 27433 reads

====================================================================================

graph has 14587 edges initially, 327 edges after simplification

total ucounts = 1242
nonsolo ucounts = 901[2^113, 3^95, 4^66, 5^50, 6^42, 7^54, 8^43, 9^41, 10^32, 11^42, 12^30, 13^29, 14^21, 15^19, 16^11, 17^21, 18^16, 19^17, 20^17, 21^14, 22^12, 23^14, 24^6, 25^10, 26^7, 27^6, 28^4, 29^4, 30^4, 31^2, 32, 33^2, 34^2, 36, 37^3, 38, 39^3, 41^2, 42, 43, 44^2, 45, 46, 47, 48^2, 49, 54, 55, 57^2, 60^2, 61, 62, 63, 65, 66, 67, 68, 70, 71^2, 72, 82, 83, 92, 93^2, 96, 108, 113, 114, 115, 119, 125, 150, 1308, 2534, 5573, 6231]
surviving nonsolo ucounts = 4[1308, 2534, 5573, 6231]
ids = [25, 771, 920, 342]

====================================================================================

UMI info for barcode CGAGAAGAGTTAGCGG-1 contig 1 = GCCTCTGAGA...
umi AAAGTGCCAG = 1310 reads: +400 validated
umi GCTTGTGCTT = 2380 reads: -361 +3 -1XX +3 -1XX +3 -3XX +2 -1XX +2 -4XX +2 -4XX +2 -1XX +1 -2XX +4 invalidated

GOOD CONTIGS

TIG 1[bases=500]
0-58 ==> 11-69 on |478|TRAV20|5'UTR| [len=69] (mis=0)
58-395 ==> 0-337 on |479|TRAV20|L-REGION+V-REGION| [len=337] (mis=0)
403-458 ==> 8-63 on |444|TRAJ58|J-REGION| [len=63] (mis=0)
458-500 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 1 umis using 80 reads
cdr3 = CAVQGLLTSGSRLTF at 382, score = 8 + 9
umis assigned: [25, 771]
of which 2 are surviving nonsolos
reads assigned: 3664
start codons at 58, 67
confident = true
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.569 = CGAGAAGCACACATGT-1

using 98 reads

====================================================================================

graph has 66 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 5[2, 3, 6, 8, 77]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.573 = CGAGAAGCACGAGGTA-1

using 59 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[12, 47]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.575 = CGAGAAGCACGGCTAC-1

using 109 reads

====================================================================================

graph has 70 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[16, 93]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.587 = CGAGAAGCATCCTTGC-1

using 402 reads

====================================================================================

graph has 215 edges initially, 12 edges after simplification

total ucounts = 20
nonsolo ucounts = 18[2^3, 3, 4, 5^2, 6, 24^2, 25, 26, 27, 29, 43, 45, 49, 79]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.588 = CGAGAAGCATCGGGTC-1

using 34 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.594 = CGAGAAGGTTGTACAC-1

using 28 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.596 = CGAGAAGTCAACACTG-1

using 61 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[61]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.601 = CGAGAAGTCACAACGT-1

using 96 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[96]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.605 = CGAGAAGTCCACTGGG-1

using 29 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.611 = CGAGAAGTCCTGCTTG-1

using 31 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.620 = CGAGAAGTCTAACTCT-1

using 42 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[42]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.623 = CGAGAAGTCTGCAAGT-1

using 22 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 1[20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.625 = CGAGAAGTCTGCCAGG-1

using 24709 reads

====================================================================================

graph has 8414 edges initially, 94 edges after simplification

total ucounts = 358
nonsolo ucounts = 250[2^42, 3^25, 4^29, 5^12, 6^18, 7^10, 8^9, 9^10, 10^9, 11^8, 12^8, 13^5, 14^4, 15^4, 16^10, 17^4, 18^6, 19, 20^4, 21, 22, 23, 24^2, 27, 30, 31^2, 37, 38, 49, 59, 83, 98, 125, 237, 599, 637, 757, 781, 868, 961, 1004, 1015, 1298, 2080, 2209, 2272, 2324, 2502, 2770]
surviving nonsolo ucounts = 16[237, 599, 637, 757, 781, 868, 961, 1004, 1015, 1298, 2080, 2209, 2272, 2324, 2502, 2770]
ids = [310, 8, 256, 98, 144, 274, 240, 132, 312, 347, ...]

====================================================================================

UMI info for barcode CGAGAAGTCTGCCAGG-1 contig 1 = GGGGCTTCAA...
umi AACGGTTTGT = 602 reads: +394 validated
umi ACGCTCGAAG = 2516 reads: +394 validated
umi ATGATACCAT = 2063 reads: +394 validated
umi ATGTCGGTAC = 2216 reads: +394 validated
umi CCTGCCTATT = 672 reads: +394 validated
umi CGCGGTTTGG = 2292 reads: +394 validated
umi GCATAGCATA = 2760 reads: +394 validated
umi GGTTTTAGGC = 894 reads: -358X +3 -1X +9 -1XX +2 -1XX +2 -2XX +7 -1XX +2 -3XX +2 invalidated
umi TACACTTTGA = 588 reads: -358 +3 -1X +9 -1X +2 -1X +2 -2XX +7 -1XX +2 -3XX +2 invalidated
umi TCTCCCACTC = 2323 reads: +394 validated
umi TGATTTTCTA = 203 reads: -382 +4 -1XX +2 -3XX +2 invalidated
umi TGCACGACTC = 1018 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=463]
17-361 ==> 0-344 on |613|TRBV28|L-REGION+V-REGION| [len=344] (mis=4)
359-375 ==> 0-16 on |730|TRBD2|D-REGION| [len=16] (mis=1)
375-411 ==> 11-47 on |550|TRBJ2-7|J-REGION| [len=47] (mis=0)
411-463 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 9 umis using 1222 reads
cdr3 = CASSPGTSGSEYF at 344, score = 6 + 8
umis assigned: [8, 36, 82, 88, 144, 155, 207, 240, 256, 302] and 2 others
of which 12 are surviving nonsolos
reads assigned: 17837
start codons at 17, 75, 139, 152, 159, 167, 222, 225, 233, 335
confident = true

REJECT CONTIGS

TIG 1[bases=512]
0-45 ==> 0-45 on |484|TRAV23/DV6|5'UTR| [len=45] (mis=0)
45-409 ==> 0-364 on |485|TRAV23/DV6|L-REGION+V-REGION| [len=364] (mis=5)
409-470 ==> 0-61 on |427|TRAJ40|J-REGION| [len=61] (mis=3)
470-512 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
umis assigned: [274, 347]
of which 2 are surviving nonsolos
reads assigned: 2082
start codons at 45, 92, 202, 230, 286, 360
confident = false
frameshifted full length stopped transcript of length 512
did not find CDR3
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.629 = CGAGAAGTCTTCGGTC-1

using 51 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[51]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.633 = CGAGCACAGAAGAAGC-1

using 193 reads

====================================================================================

graph has 166 edges initially, 10 edges after simplification

total ucounts = 20
nonsolo ucounts = 15[2^2, 3, 4, 5, 6^2, 7, 11, 14, 16, 17, 18, 31, 46]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.637 = CGAGCACAGACGCAAC-1

using 43 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[43]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.639 = CGAGCACAGATCGATA-1

using 64 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[64]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.640 = CGAGCACAGATGAGAG-1

using 35 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.647 = CGAGCACAGCGGATCA-1

using 37 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.648 = CGAGCACAGCTAAACA-1

using 95 reads

====================================================================================

graph has 52 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[95]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.656 = CGAGCACAGGGTGTGT-1

using 77 reads

====================================================================================

graph has 60 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[77]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.658 = CGAGCACAGGTGTTAA-1

using 29 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.660 = CGAGCACAGTAGCCGA-1

using 62 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[62]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.662 = CGAGCACAGTATCGAA-1

using 875 reads

====================================================================================

graph has 622 edges initially, 24 edges after simplification

total ucounts = 54
nonsolo ucounts = 43[2^7, 3^5, 4^2, 5, 7^3, 10, 12, 13^2, 14^2, 15^2, 16, 18, 23, 24, 25, 29, 33, 34, 36^2, 39, 41, 46, 48, 56, 87, 104]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.664 = CGAGCACAGTGCCAGA-1

using 13 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[13]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.668 = CGAGCACCAAATACAG-1

using 131 reads

====================================================================================

graph has 76 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 4[3, 8, 20, 97]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.669 = CGAGCACCAACACCTA-1

using 445 reads

====================================================================================

graph has 306 edges initially, 14 edges after simplification

total ucounts = 25
nonsolo ucounts = 19[2^3, 6, 8^2, 11, 12, 13, 16, 21, 24, 25, 34, 39, 47, 55, 57^2]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.670 = CGAGCACCAACACGCC-1

using 72 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[2, 3, 65]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.675 = CGAGCACCAATGGACG-1

using 47 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[47]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.678 = CGAGCACCACCAGGCT-1

using 20 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.683 = CGAGCACCACGGTAAG-1

using 3765 reads

====================================================================================

graph has 4465 edges initially, 74 edges after simplification

total ucounts = 667
nonsolo ucounts = 473[2^86, 3^65, 4^46, 5^51, 6^38, 7^25, 8^22, 9^22, 10^18, 11^12, 12^14, 13^11, 14^7, 15^9, 16^8, 17^5, 18^3, 19^4, 20^5, 21^3, 22^2, 23^2, 24, 25^3, 26^2, 28, 29, 31, 32, 33^2, 38, 62, 99]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.695 = CGAGCACCAGGTCGTC-1

using 81 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[6, 74]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.698 = CGAGCACCAGTCAGAG-1

using 37 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.699 = CGAGCACCAGTGGGAT-1

using 49 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[48]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.701 = CGAGCACCATAACCTG-1

using 28 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.704 = CGAGCACCATGTTGAC-1

using 60 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[60]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.707 = CGAGCACGTAAGTGGC-1

using 27 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.708 = CGAGCACGTAATCACC-1

using 186 reads

====================================================================================

graph has 168 edges initially, 2 edges after simplification

total ucounts = 12
nonsolo ucounts = 9[2^2, 3, 4, 6, 12, 18, 27, 109]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.716 = CGAGCACGTAGCACGA-1

using 7741 reads

====================================================================================

graph has 2734 edges initially, 30 edges after simplification

total ucounts = 217
nonsolo ucounts = 138[2^42, 3^9, 4^10, 5^10, 6^8, 7^9, 8^10, 9^3, 10^5, 11^4, 12^2, 13^2, 14, 15, 16^2, 18^3, 20^2, 22, 23, 24^3, 27, 30, 39, 40, 41, 46, 52, 60, 83, 6409]
surviving nonsolo ucounts = 1[6409]
ids = [211]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.721 = CGAGCACGTCACTTCC-1

using 57 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[57]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.722 = CGAGCACGTCAGGACA-1

using 55 reads

====================================================================================

graph has 81 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[8, 47]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.736 = CGAGCACGTGTCGCTG-1

using 39 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[39]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.737 = CGAGCACGTTAAGGGC-1

using 1866 reads

====================================================================================

graph has 1208 edges initially, 22 edges after simplification

total ucounts = 127
nonsolo ucounts = 87[2^19, 3^14, 4^9, 5^7, 6^4, 7, 8^8, 9^3, 11^2, 12, 14^4, 15^2, 16^2, 19, 20, 21, 24, 25, 32, 33, 37, 41, 52, 1097]
surviving nonsolo ucounts = 1[1097]
ids = [119]

====================================================================================

UMI info for barcode CGAGCACGTTAAGGGC-1 contig 1 = GTTCCCCTAT...
umi TTCAACTCGT = 1102 reads: +391 validated

GOOD CONTIGS

TIG 1[bases=500]
0-57 ==> 0-57 on |647|TRBV6-1|5'UTR| [len=57] (mis=0)
57-401 ==> 0-344 on |648|TRBV6-1|L-REGION+V-REGION| [len=344] (mis=4)
400-448 ==> 0-48 on |548|TRBJ2-5|J-REGION| [len=48] (mis=4)
448-500 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 1 umis using 74 reads
cdr3 = CASSPILETQYF at 384, score = 9 + 7
umis assigned: [119]
of which 1 are surviving nonsolos
reads assigned: 1078
start codons at 15, 57, 115, 168, 192, 207, 234, 298, 307
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.739 = CGAGCACGTTAGTGGG-1

using 18 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.740 = CGAGCACGTTATCACG-1

using 309 reads

====================================================================================

graph has 182 edges initially, 12 edges after simplification

total ucounts = 14
nonsolo ucounts = 14[2, 4^3, 5^2, 9, 12, 25, 26, 40, 43, 61, 69]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.742 = CGAGCACGTTCCAACA-1

using 43 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[43]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.743 = CGAGCACGTTCCCTTG-1

using 65 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[65]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.747 = CGAGCACTCAATACCG-1

using 84 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[84]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.750 = CGAGCACTCAGCTTAG-1

using 31 reads

====================================================================================

graph has 52 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.761 = CGAGCACTCCGATATG-1

using 89 reads

====================================================================================

graph has 48 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[88]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.763 = CGAGCACTCCTCAATT-1

using 80 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[80]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.764 = CGAGCACTCCTCCTAG-1

using 69 reads

====================================================================================

graph has 102 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[4, 64]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.767 = CGAGCACTCCTTGGTC-1

using 24 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 1[22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.772 = CGAGCACTCGATAGAA-1

using 16943 reads

====================================================================================

graph has 9966 edges initially, 214 edges after simplification

total ucounts = 980
nonsolo ucounts = 699[2^114, 3^78, 4^68, 5^59, 6^53, 7^27, 8^34, 9^26, 10^23, 11^27, 12^20, 13^13, 14^17, 15^17, 16^12, 17^14, 18^13, 19^12, 20^3, 21^10, 22^4, 23^2, 24^6, 25^3, 26^2, 27, 28^4, 29^2, 30^2, 31^4, 32^2, 33, 34^2, 35, 37, 38, 45, 46, 50, 51, 60, 63, 64, 68, 73, 91, 105^2, 122, 163, 208, 403, 542, 963, 1239, 1405, 5102]
surviving nonsolo ucounts = 8[73, 163, 403, 542, 963, 1239, 1405, 5102]
ids = [594, 425, 845, 727, 2, 730, 193, 108]

====================================================================================

UMI info for barcode CGAGCACTCGATAGAA-1 contig 1 = AGCTGTGAGG...
umi CATTAATCCT = 5127 reads: -145X +1 -2XX +252 invalidated
umi CTTCGCTTCC = 158 reads: +400 validated
umi TGCAGTCGTT = 277 reads: -119X +281 invalidated

UMI info for barcode CGAGCACTCGATAGAA-1 contig 2 = TGGGGAGAGT...
umi AAATAGAGTG = 971 reads: +391 validated
umi CCGCATCACC = 1420 reads: +391 validated
umi TATTTCCCTT = 527 reads: +391 validated

GOOD CONTIGS

TIG 1[bases=533]
0-81 ==> 0-81 on |585|TRBV18|5'UTR| [len=81] (mis=0)
81-428 ==> 0-347 on |586|TRBV18|L-REGION+V-REGION| [len=347] (mis=2)
425-437 ==> 0-12 on |535|TRBD1|D-REGION| [len=12] (mis=2)
441-481 ==> 8-48 on |537|TRBJ1-2|J-REGION| [len=48] (mis=0)
481-533 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 3 umis using 713 reads
cdr3 = CASSPRQGARGYTF at 411, score = 7 + 9
umis assigned: [108, 425, 845]
of which 3 are surviving nonsolos
reads assigned: 5467
start codons at 66, 81, 139, 150, 203, 213, 229, 273, 304, 315
confident = true

TIG 2[bases=517]
84-420 ==> 0-336 on |481|TRAV21|L-REGION+V-REGION| [len=336] (mis=4)
412-475 ==> 0-63 on |443|TRAJ57|J-REGION| [len=63] (mis=4)
475-517 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 3 umis using 177 reads
cdr3 = CAAQGGSEKLVF at 408, score = 9 + 9
umis assigned: [2, 193, 727]
of which 3 are surviving nonsolos
reads assigned: 2856
start codons at 84, 128, 328
confident = true

REJECT CONTIGS

TIG 1[bases=502]
0-46 ==> 0-46 on |661|TRBV6-6|5'UTR| [len=46] (mis=0)
46-390 ==> 0-344 on |662|TRBV6-6|L-REGION+V-REGION| [len=344] (mis=3)
387-403 ==> 0-16 on |730|TRBD2|D-REGION| [len=16] (mis=2)
408-450 ==> 8-50 on |543|TRBJ2-1|J-REGION| [len=50] (mis=0)
450-502 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
umis assigned: [730]
of which 1 are surviving nonsolos
reads assigned: 1201
start codons at 4, 46, 104, 157, 181, 196, 223, 287, 408
confident = false
did not find CDR3
now this is a cell
paired!

GCTTCTCAGCCTGGTGACTCAGCCACCTACCTCTGTGCTGCGCAGGGCGGATCTGAAAAGCTGGTCTTTGGAAAGGGAACGAAACTGACAGTAAACCCAT <==> GTAGTGCGAGGAGATTCGGCAGCTTATTTCTGTGCCAGCTCACCTCGACAGGGGGCGCGGGGCTACACCTTCGGTTCGGGGACCAGGTTAACCGTTGTAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.773 = CGAGCACTCGCCTGAG-1

using 87 reads

====================================================================================

graph has 56 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[2, 3, 6, 74]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.774 = CGAGCACTCGGAAACG-1

using 52 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[52]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.775 = CGAGCACTCGGTTCGG-1

using 106 reads

====================================================================================

graph has 88 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[5, 100]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.776 = CGAGCACTCGTGACAT-1

using 34 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.781 = CGAGCACTCTATGTGG-1

using 64 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[64]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.782 = CGAGCACTCTCGAGTA-1

using 53 reads

====================================================================================

graph has 72 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[9, 12, 31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.789 = CGAGCACTCTTCAACT-1

using 4275 reads

====================================================================================

graph has 4984 edges initially, 122 edges after simplification

total ucounts = 631
nonsolo ucounts = 480[2^83, 3^50, 4^48, 5^37, 6^37, 7^31, 8^25, 9^27, 10^18, 11^23, 12^18, 13^8, 14^10, 15^7, 16^7, 17^10, 18^5, 19^5, 20^2, 21^3, 22^3, 23, 24^2, 25^2, 26^2, 27^2, 28^3, 32, 33^2, 37^2, 39, 49, 55, 60, 78, 151]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.798 = CGAGCCAAGAATTCCC-1

using 21 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.800 = CGAGCCAAGACAATAC-1

using 223 reads

====================================================================================

graph has 273 edges initially, 6 edges after simplification

total ucounts = 22
nonsolo ucounts = 17[2^4, 6^2, 8, 11^2, 15, 18, 19^2, 21, 23, 26, 27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.803 = CGAGCCAAGACTCGGA-1

using 45556 reads

====================================================================================

graph has 25460 edges initially, 836 edges after simplification

total ucounts = 1608
nonsolo ucounts = 1390[2^114, 3^95, 4^76, 5^49, 6^57, 7^50, 8^34, 9^57, 10^26, 11^24, 12^28, 13^22, 14^27, 15^31, 16^19, 17^17, 18^22, 19^18, 20^14, 21^17, 22^14, 23^9, 24^15, 25^15, 26^15, 27^13, 28^17, 29^11, 30^15, 31^9, 32^11, 33^10, 34^16, 35^10, 36^8, 37^11, 38^10, 39^13, 40^6, 41^8, 42^11, 43^8, 44^12, 45^10, 46^8, 47^10, 48^4, 49^11, 50^6, 51^5, 52^4, 53^11, 54^5, 55^5, 56^12, 57^4, 58^9, 59^6, 60^8, 61^10, 62^7, 63^3, 64^5, 65^7, 66, 67^4, 68^8, 69^8, 70^5, 71^2, 72^8, 73^4, 74^7, 75^4, 76^2, 77^7, 78^6, 79^6, 80^6, 81^6, 82^9, 83^4, 84^2, 85^3, 86^5, 87^3, 88^5, 89^2, 90^2, 91^6, 92^5, 93, 94^2, 95, 96^2, 98^2, 99^3, 100^4, 101^3, 104, 105, 106^3, 107, 109^3, 112^3, 113, 114^2, 117, 118^2, 119, 121, 129, 130, 136, 140, 146^2, 191, 6885]
surviving nonsolo ucounts = 1[6885]
ids = [1533]

====================================================================================

REJECT CONTIGS

TIG 1[bases=358]
1-105 ==> 0-104 on segment before TRBJ2-3 exon 1 [len=104] (mis=4)
105-154 ==> 0-49 on |546|TRBJ2-3|J-REGION| [len=49] (mis=0)
154-256 ==> 0-102 on segment before TRBJ2-4 exon 1 [len=102] (mis=0)
306-358 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
umis assigned: [533, 608, 1533, 1595]
of which 1 are surviving nonsolos
reads assigned: 6768
start codons at 15
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.808 = CGAGCCAAGATAGGAG-1

using 6674 reads

====================================================================================

graph has 4167 edges initially, 157 edges after simplification

total ucounts = 444
nonsolo ucounts = 362[2^27, 3^28, 4^19, 5^11, 6^14, 7^13, 8^9, 9^11, 10^8, 11^8, 12^10, 13^10, 14^13, 15^5, 16^6, 17^8, 18^5, 19^9, 20^16, 21^14, 22^8, 23^4, 24^7, 25^9, 26^5, 27^4, 28^6, 29^3, 30^6, 31^3, 32^3, 33^3, 34^6, 35, 36^2, 37^4, 38, 39^4, 40^2, 41^2, 42^2, 43^2, 44^3, 45^5, 46^2, 47^4, 48^2, 49^2, 52^2, 53^4, 54, 55, 57, 59, 64, 74, 78]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.811 = CGAGCCAAGATGGCGT-1

using 16758 reads

====================================================================================

graph has 7795 edges initially, 164 edges after simplification

total ucounts = 836
nonsolo ucounts = 607[2^106, 3^86, 4^54, 5^43, 6^50, 7^39, 8^24, 9^24, 10^13, 11^14, 12^12, 13^20, 14^10, 15^12, 16^10, 17^10, 18^6, 19^6, 20^8, 21^3, 22^3, 23^3, 24, 25^3, 26^3, 27, 28^3, 30^3, 31^4, 32^2, 33^3, 34, 35, 36, 37, 41^2, 43, 46, 47, 51^2, 56, 58, 60, 62, 68^2, 71, 73, 74, 81, 92, 105, 109, 113, 429, 3804, 6279]
surviving nonsolo ucounts = 3[429, 3804, 6279]
ids = [670, 660, 484]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.816 = CGAGCCAAGCCCAACC-1

using 7220 reads

====================================================================================

graph has 3398 edges initially, 70 edges after simplification

total ucounts = 294
nonsolo ucounts = 178[2^45, 3^28, 4^15, 5^14, 6^6, 7^13, 8, 9^5, 10, 11^3, 12, 13^3, 14^2, 15^3, 17^3, 19^3, 23, 26^2, 27, 32, 33, 37, 40^2, 45, 48^2, 49, 51, 53^2, 57, 60, 79, 83, 87, 88, 94, 111, 165, 424, 557, 572, 662, 691, 908, 1076]
surviving nonsolo ucounts = 9[60, 165, 424, 557, 572, 662, 691, 908, 1076]
ids = [111, 67, 7, 188, 39, 102, 68, 65, 231]

====================================================================================

UMI info for barcode CGAGCCAAGCCCAACC-1 contig 1 = GACTCTGCTC...
umi ACTATTGAGT = 577 reads: +394 validated
umi ATCATATGAG = 917 reads: +394 validated
umi ATCTCTATTA = 164 reads: +335 -1 +51 -1 +6 non-validated
umi ATGAAGCTCG = 703 reads: +394 validated
umi CACCGAGGTG = 1 reads: -390 +2 -2X invalidated
umi CCAATACTAG = 663 reads: +394 validated
umi CCCCAATTTC = 59 reads: +346 -43 +5 non-validated
umi GTCGAGTCTA = 563 reads: +394 validated
umi TCCGCTTACG = 996 reads: -371X +1 -7XX +2 -12XX +1 invalidated

GOOD CONTIGS

TIG 1[bases=642]
0-196 ==> 12-208 on |589|TRBV19|5'UTR| [len=208] (mis=0)
196-540 ==> 0-344 on |590|TRBV19|L-REGION+V-REGION| [len=344] (mis=4)
552-590 ==> 12-50 on |547|TRBJ2-4|J-REGION| [len=50] (mis=0)
590-642 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 5 umis using 83 reads
cdr3 = CASSPGLEIRQYF at 523, score = 6 + 8
umis assigned: [39, 65, 67, 68, 80, 102, 111, 188, 231]
of which 8 are surviving nonsolos
reads assigned: 4562
start codons at 196, 254, 305, 341, 346, 410
confident = true
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.818 = CGAGCCAAGCGTTCCG-1

using 32 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.819 = CGAGCCAAGCGTTTAC-1

using 6960 reads

====================================================================================

graph has 4911 edges initially, 148 edges after simplification

total ucounts = 832
nonsolo ucounts = 589[2^120, 3^81, 4^57, 5^55, 6^35, 7^26, 8^23, 9^35, 10^18, 11^19, 12^14, 13^8, 14^10, 15^12, 16^10, 17^6, 18^7, 19^7, 20^4, 21^2, 22^2, 23, 24, 25, 26^3, 27^3, 29^2, 30^2, 31, 34, 36^2, 39^2, 40, 41^3, 45, 47, 49^3, 52, 53^2, 55, 58, 65, 66, 67, 91, 1645]
surviving nonsolo ucounts = 1[1645]
ids = [414]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.822 = CGAGCCAAGCTATGCT-1

using 50 reads

====================================================================================

graph has 72 edges initially, 2 edges after simplification

total ucounts = 13
nonsolo ucounts = 9[2^2, 3^5, 4, 23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.828 = CGAGCCAAGGACACCA-1

using 68 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[68]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.832 = CGAGCCAAGGCTCTTA-1

using 56 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 5[2, 4, 8, 11, 31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.834 = CGAGCCAAGGTGATTA-1

using 48 reads

====================================================================================

graph has 27 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[48]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.837 = CGAGCCAAGTAGCGGT-1

using 77 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[2, 3, 70]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.838 = CGAGCCAAGTCCAGGA-1

using 52 reads

====================================================================================

graph has 48 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[52]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.843 = CGAGCCAAGTGGAGAA-1

using 138 reads

====================================================================================

graph has 104 edges initially, 6 edges after simplification

total ucounts = 9
nonsolo ucounts = 7[3, 4, 5, 10, 22, 30, 62]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.844 = CGAGCCAAGTGTACGG-1

using 20 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.849 = CGAGCCACAAACAACA-1

using 119 reads

====================================================================================

graph has 58 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[119]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.851 = CGAGCCACAACACCTA-1

using 36 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.856 = CGAGCCACAAGGACTG-1

using 6277 reads

====================================================================================

graph has 4741 edges initially, 110 edges after simplification

total ucounts = 623
nonsolo ucounts = 426[2^76, 3^52, 4^51, 5^33, 6^24, 7^15, 8^16, 9^20, 10^18, 11^12, 12^18, 13^3, 14^9, 15^10, 16^8, 17^6, 18^3, 19^8, 20^6, 21, 23^4, 24, 25^2, 26^4, 28, 29^3, 30^3, 35, 36, 37, 39, 40, 42, 51^2, 53, 55, 66, 71, 76, 81, 102, 104, 110, 126, 1776]
surviving nonsolo ucounts = 1[1776]
ids = [573]

====================================================================================

REJECT CONTIGS

TIG 1[bases=491]
39-111 ==> 0-72 on segment before TRBJ2-6 exon 1 [len=72] (mis=0)
111-164 ==> 0-53 on |549|TRBJ2-6|J-REGION| [len=53] (mis=0)
164-328 ==> 0-164 on segment before TRBJ2-7 exon 1 [len=164] (mis=0)
439-491 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
umis assigned: [573]
of which 1 are surviving nonsolos
reads assigned: 1760
start codons at 307
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.862 = CGAGCCACACATAACC-1

using 49 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[49]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.865 = CGAGCCACACCAGTTA-1

using 5537 reads

====================================================================================

graph has 5731 edges initially, 116 edges after simplification

total ucounts = 915
nonsolo ucounts = 632[2^118, 3^96, 4^67, 5^47, 6^47, 7^41, 8^38, 9^18, 10^23, 11^19, 12^19, 13^17, 14^11, 15^14, 16^6, 17^6, 18^6, 19^7, 20^2, 21^3, 22, 23^2, 24^2, 25, 26, 27, 28, 29, 31, 36^2, 42^2, 52, 65, 66, 72^2, 74, 76, 90, 93, 98, 101^2]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.872 = CGAGCCACACTTGGAT-1

using 48 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[48]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.874 = CGAGCCACAGACGTAG-1

using 17 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[17]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.878 = CGAGCCACAGATGGCA-1

using 67 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[67]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.879 = CGAGCCACAGATGGGT-1

using 66 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[66]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.892 = CGAGCCACATACTCTT-1

using 81 reads

====================================================================================

graph has 46 edges initially, 4 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[2, 4, 26, 47]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.896 = CGAGCCACATCCCACT-1

using 72 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 3[2, 3, 64]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.903 = CGAGCCACATTGTGCA-1

using 199 reads

====================================================================================

graph has 80 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[199]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.904 = CGAGCCACATTTCACT-1

using 14692 reads

====================================================================================

graph has 14434 edges initially, 391 edges after simplification

total ucounts = 2163
nonsolo ucounts = 1608[2^267, 3^203, 4^167, 5^139, 6^112, 7^86, 8^85, 9^64, 10^63, 11^64, 12^46, 13^36, 14^33, 15^29, 16^26, 17^18, 18^23, 19^17, 20^16, 21^19, 22^9, 23^7, 24^6, 25^6, 26^11, 27^4, 28^5, 29^4, 30^5, 32, 33, 34^2, 35^2, 36, 37, 39^2, 40^3, 41, 42, 43, 47, 48^3, 49, 51, 52, 59, 64, 66, 67^2, 68, 77^2, 81, 84, 89^2, 103, 104, 181]
surviving nonsolo ucounts = 8[2^2, 3^2, 7, 12, 34, 181]
ids = [897, 939, 77, 388, 428, 390, 211, 604]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.914 = CGAGCCAGTACTTAGC-1

using 39 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[39]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.916 = CGAGCCAGTAGATTAG-1

using 86 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[86]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.918 = CGAGCCAGTCAAACTC-1

using 54 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[54]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.922 = CGAGCCAGTCAGGACA-1

using 93 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[93]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.924 = CGAGCCAGTCATCCCT-1

using 1819 reads

====================================================================================

graph has 1460 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[1819]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.931 = CGAGCCAGTCGCGAAA-1

using 187 reads

====================================================================================

graph has 64 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[5, 8, 172]
surviving nonsolo ucounts = 1[172]
ids = [0]

====================================================================================

REJECT CONTIGS

TIG 1[bases=438]
0-53 ==> 6-59 on |567|TRBV12-4|5'UTR| [len=59] (mis=0)
53-400 ==> 0-347 on |568|TRBV12-4|L-REGION+V-REGION| [len=347] (mis=1)
414-438 ==> 0-24 on |548|TRBJ2-5|J-REGION| [len=48] (mis=1)
cdr3 = CASSLVG at 383, score = 8 + 4
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 126
start codons at 1, 53, 111, 152, 175, 224, 227, 276, 287, 314, 321
confident = false
not full
VJ delta = -13
not full
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.935 = CGAGCCAGTCTTGCGG-1

using 89 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[88]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.937 = CGAGCCAGTGATGCCC-1

using 47 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[47]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.940 = CGAGCCAGTGCCTGTG-1

using 261 reads

====================================================================================

graph has 226 edges initially, 6 edges after simplification

total ucounts = 12
nonsolo ucounts = 8[3^2, 6^2, 23, 49, 72, 95]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.941 = CGAGCCAGTGCCTTGG-1

using 45 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[44]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.942 = CGAGCCAGTGCTCTTC-1

using 64 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[64]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.952 = CGAGCCAGTTACTGAC-1

using 33 reads

====================================================================================

graph has 52 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 6[2^2, 5, 6, 8, 9]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.955 = CGAGCCAGTTCAGTAC-1

using 68 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[68]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.956 = CGAGCCAGTTCATGGT-1

using 61 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[3, 6, 51]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.957 = CGAGCCAGTTCCACAA-1

using 71 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[71]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.970 = CGAGCCATCACATACG-1

using 20 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.975 = CGAGCCATCATAGCAC-1

using 78 reads

====================================================================================

graph has 60 edges initially, 4 edges after simplification

total ucounts = 14
nonsolo ucounts = 11[2^3, 4^2, 5, 6, 8^2, 9, 25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.976 = CGAGCCATCATCACCC-1

using 381 reads

====================================================================================

graph has 238 edges initially, 8 edges after simplification

total ucounts = 23
nonsolo ucounts = 23[2^3, 3^3, 4, 5^2, 6^2, 7, 10, 11, 12, 14, 15, 20, 29, 44, 51, 62, 65]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.977 = CGAGCCATCATCTGTT-1

using 19 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.978 = CGAGCCATCATTCACT-1

using 89 reads

====================================================================================

graph has 96 edges initially, 2 edges after simplification

total ucounts = 13
nonsolo ucounts = 8[2, 5^2, 6, 7, 8^2, 43]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.987 = CGAGCCATCCCTTGCA-1

using 26 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.988 = CGAGCCATCCGCGCAA-1

using 38 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.994 = CGAGCCATCGCAAGCC-1

using 54 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[54]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.998 = CGAGCCATCTCAACTT-1

using 366 reads

====================================================================================

graph has 308 edges initially, 8 edges after simplification

total ucounts = 24
nonsolo ucounts = 16[2^2, 3^2, 4, 5, 8, 12, 13^2, 19, 33, 50, 51, 66, 74]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 35.1001 = CGAGCCATCTCCGGTT-1

using 109 reads

====================================================================================

graph has 118 edges initially, 4 edges after simplification

total ucounts = 14
nonsolo ucounts = 9[2, 4^3, 13, 14, 15, 23, 25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!
sorting bam, mem = 0.16
running samtools sort -l 8G -o /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_T_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk35-ucc6a7a72e6/files/contig_bam_sorted.bam /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_T_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk35-ucc6a7a72e6/files/contig_bam.bam

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

81.180 seconds used processing barcodes, peak mem = 0.34
