[stdout]

starting vdj_asm_asm, mem = 0.01
available mem = 832.94 GB
/scratch/etanis/software/cellranger-6.1.2/lib/bin/cr_vdj martian assembly main /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_T_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk16-ucc6a7a72e6 /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_T_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk16-ucc6a7a72e6/files /scratch/etanis/ST-118/multi/dimitri-multi/journal/SC_MULTI_CS.SC_MULTI_CORE.MULTI_GEM_WELL_PROCESSOR.VDJ_T_GEM_WELL_PROCESSOR.SC_VDJ_CONTIG_ASSEMBLER.ASSEMBLE_VDJ.fork0.chnk16.ucc6a7a72e6
n50_n50_rpu = 2159

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.0 = AGTCTTTTCTTCGGTC-1

using 47905 reads

====================================================================================

graph has 16607 edges initially, 396 edges after simplification

total ucounts = 1398
nonsolo ucounts = 1072[2^130, 3^140, 4^86, 5^69, 6^75, 7^58, 8^39, 9^41, 10^34, 11^40, 12^30, 13^32, 14^21, 15^30, 16^14, 17^20, 18^13, 19^21, 20^17, 21^9, 22^19, 23^10, 24^10, 25^9, 26^9, 27^5, 28^6, 29^7, 30^4, 31^2, 32^5, 33^5, 34, 36^2, 37^5, 38^3, 39^2, 41^4, 42, 46, 47^2, 48, 49, 52, 53, 54, 55, 58^2, 59, 62, 66, 67, 71, 77, 81, 84, 89, 100, 109, 382, 666, 843, 867, 977, 1011, 1153, 1206, 1247, 1650, 1778, 1896, 1976, 2037, 2066, 2117, 2147, 2152, 2233, 2244, 2587, 2930]
surviving nonsolo ucounts = 22[382, 666, 843, 867, 977, 1011, 1153, 1206, 1247, 1650, 1778, 1896, 1976, 2037, 2066, 2117, 2147, 2152, 2233, 2244, 2587, 2930]
ids = [222, 391, 1315, 831, 597, 1296, 1002, 183, 31, 536, ...]

====================================================================================

UMI info for barcode AGTCTTTTCTTCGGTC-1 contig 1 = AGAGGCCCCA...
umi AACGAGTTCA = 1246 reads: +403 validated
umi AATGGGCTCT = 2575 reads: +403 validated
umi ACAAACCCGC = 2248 reads: +403 validated
umi ACTTATATTC = 1218 reads: +403 validated
umi AGCCGGTGGT = 2142 reads: +403 validated
umi AGGATGCCTC = 382 reads: +403 validated
umi CAATTCTCAC = 1785 reads: +403 validated
umi CACGACGGGC = 2237 reads: +403 validated
umi CCTCAGACAA = 2952 reads: +403 validated
umi CGGTGCTCCA = 1664 reads: +395 -6X +1 -1XX invalidated
umi CTCGATACCG = 152 reads: -379X +1 -1X +2 -1X +3 -1XX +5 -1XX +1 -1XX +2 -4XX +1 invalidated
umi CTTTTATGTC = 2076 reads: +403 validated
umi CTTTTTCTTG = 2115 reads: +403 validated
umi GCTGGCGATA = 2174 reads: +403 validated
umi GGACAATTCT = 1923 reads: +403 validated
umi TAAGTCCTTC = 1984 reads: +403 validated
umi TAGCCATCGA = 1154 reads: +403 validated
umi TCCCATGTCC = 2039 reads: +403 validated
umi TTCCTCTGTG = 1015 reads: +403 validated

UMI info for barcode AGTCTTTTCTTCGGTC-1 contig 2 = CTGGAAGACC...
umi CACGATCTTT = 670 reads: +424 validated
umi CTTACACCGC = 3 reads: -412X +1 -1XX +2 -1XX +1 -1XX +1 -1XX +1 -2XX invalidated
umi GGGGTGTTGT = 848 reads: +424 validated
umi TTCTTCCAGG = 791 reads: +424 validated

GOOD CONTIGS

TIG 1[bases=484]
0-29 ==> 0-29 on |622|TRBV4-1|5'UTR| [len=29] (mis=0)
29-373 ==> 0-344 on |623|TRBV4-1|L-REGION+V-REGION| [len=344] (mis=4)
382-432 ==> 0-50 on |547|TRBJ2-4|J-REGION| [len=50] (mis=5)
432-484 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 18 umis using 2050 reads
cdr3 = CASSLSQTSGSNIQYF at 356, score = 10 + 8
umis assigned: [31, 81, 90, 183, 210, 222, 377, 389, 485, 536] and 9 others
of which 19 are surviving nonsolos
reads assigned: 32538
start codons at 29, 122, 128, 151, 164, 179, 221, 237, 255, 286
confident = true

TIG 2[bases=511]
0-45 ==> 0-45 on |484|TRAV23/DV6|5'UTR| [len=45] (mis=0)
45-409 ==> 0-364 on |485|TRAV23/DV6|L-REGION+V-REGION| [len=364] (mis=2)
413-469 ==> 7-63 on |443|TRAJ57|J-REGION| [len=63] (mis=0)
469-511 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 1 umis using 28 reads
cdr3 = CAASKGGGSEKLVF at 396, score = 9 + 9
umis assigned: [391, 645, 831, 1315]
of which 3 are surviving nonsolos
reads assigned: 2280
start codons at 45, 92, 202, 230, 286, 360
confident = true
now this is a cell
paired!

CAGCCTGGAGACTCAGCCACCTACTTCTGTGCAGCAAGCAAGGGGGGCGGATCTGAAAAGCTGGTCTTTGGAAAGGGAACGAAACTGACAGTAAACCCAT <==> CCAGAAGACTCAGCCCTGTATCTCTGCGCCAGCAGCCTTAGTCAAACTAGCGGGAGTAACATTCAGTACTTCGGCGCCGGGACCCGGCTCTCAGTGCTGG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.2 = AGTCTTTTCTTGGGTA-1

using 111 reads

====================================================================================

graph has 90 edges initially, 4 edges after simplification

total ucounts = 23
nonsolo ucounts = 13[2^4, 3, 4^4, 5, 10, 11, 48]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.3 = AGTCTTTTCTTTACGT-1

using 29 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.5 = AGTGAGGAGAAGATTC-1

using 71 reads

====================================================================================

graph has 96 edges initially, 2 edges after simplification

total ucounts = 12
nonsolo ucounts = 6[5^2, 8, 9, 19^2]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.7 = AGTGAGGAGAAGGTTT-1

using 125 reads

====================================================================================

graph has 134 edges initially, 4 edges after simplification

total ucounts = 15
nonsolo ucounts = 11[2^3, 3, 4^2, 8, 11^2, 17, 57]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.8 = AGTGAGGAGACATAAC-1

using 56 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[56]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.9 = AGTGAGGAGACTCGGA-1

using 20 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.10 = AGTGAGGAGAGATGAG-1

using 43 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[43]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.14 = AGTGAGGAGCAATATG-1

using 80 reads

====================================================================================

graph has 32 edges initially, 4 edges after simplification

total ucounts = 3
nonsolo ucounts = 3[2, 15, 63]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.17 = AGTGAGGAGCCACGTC-1

using 47 reads

====================================================================================

graph has 79 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 3[3, 16, 25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.18 = AGTGAGGAGCCAGAAC-1

using 72 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[72]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.19 = AGTGAGGAGCCAGTAG-1

using 68 reads

====================================================================================

graph has 86 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 7[2^2, 3, 6, 8, 20, 24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.20 = AGTGAGGAGCCCAACC-1

using 95 reads

====================================================================================

graph has 92 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 8[2, 3, 4^2, 6, 16, 17, 43]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.27 = AGTGAGGAGCTAGCCC-1

using 73 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[73]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.28 = AGTGAGGAGCTCCTCT-1

using 77 reads

====================================================================================

graph has 83 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[77]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.29 = AGTGAGGAGCTGCGAA-1

using 301 reads

====================================================================================

graph has 234 edges initially, 8 edges after simplification

total ucounts = 22
nonsolo ucounts = 18[2^3, 3^2, 5, 6, 11^2, 18, 19, 20^2, 21, 26, 32, 44, 52]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.33 = AGTGAGGAGGGAGTAA-1

using 52 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[3, 49]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.36 = AGTGAGGAGGTAAACT-1

using 362 reads

====================================================================================

graph has 222 edges initially, 10 edges after simplification

total ucounts = 16
nonsolo ucounts = 12[2^2, 3^2, 6, 18, 26, 32, 48, 66, 72, 80]
surviving nonsolo ucounts = 1[72]
ids = [2]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.37 = AGTGAGGAGGTGCACA-1

using 121 reads

====================================================================================

graph has 100 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 8[2^3, 3, 4, 8, 13, 84]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.39 = AGTGAGGAGTACATGA-1

using 135 reads

====================================================================================

graph has 137 edges initially, 6 edges after simplification

total ucounts = 12
nonsolo ucounts = 9[2, 3, 5, 7^2, 14, 16, 27, 51]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.41 = AGTGAGGAGTACGTTC-1

using 164 reads

====================================================================================

graph has 152 edges initially, 6 edges after simplification

total ucounts = 11
nonsolo ucounts = 7[2, 9, 21, 24, 30, 36, 38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.45 = AGTGAGGAGTCCTCCT-1

using 10104 reads

====================================================================================

graph has 7303 edges initially, 188 edges after simplification

total ucounts = 887
nonsolo ucounts = 663[2^99, 3^81, 4^55, 5^35, 6^30, 7^31, 8^18, 9^14, 10^16, 11^24, 12^12, 13^16, 14^14, 15^13, 16^15, 17^9, 18^19, 19^7, 20^5, 21^13, 22^10, 23^5, 24^2, 25^6, 26^6, 27^4, 28^5, 29^8, 30^3, 31^2, 32, 33^4, 34^6, 35^3, 36, 37^3, 38^5, 39^6, 40, 41^3, 42^3, 44^2, 45, 46^4, 47, 48^3, 49^2, 51, 52, 54^3, 55, 56^3, 57^2, 58^3, 59^2, 62, 63^2, 65^2, 67^2, 70, 71, 72, 78, 79, 83, 87, 98, 100, 101, 102, 125, 140, 157]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.47 = AGTGAGGAGTGTCCCG-1

using 41 reads

====================================================================================

graph has 52 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 3[3, 6, 28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.49 = AGTGAGGAGTGTGGCA-1

using 30244 reads

====================================================================================

graph has 11372 edges initially, 262 edges after simplification

total ucounts = 804
nonsolo ucounts = 618[2^82, 3^73, 4^48, 5^38, 6^29, 7^19, 8^16, 9^18, 10^11, 11^17, 12^15, 13^7, 14^13, 15^11, 16^24, 17^12, 18^14, 19^9, 20^4, 21^6, 22^10, 23^6, 24^6, 25^13, 26^6, 27^7, 28^4, 29^4, 30^3, 31^3, 32^6, 33^4, 35^2, 36^2, 37^4, 38^3, 39^3, 40^2, 41^2, 42^4, 43^2, 44^3, 45^2, 46^2, 48, 49, 50^3, 51^2, 52^4, 53, 54, 57, 58^2, 60, 62, 63, 66^2, 67, 69^2, 70, 74, 75, 80, 81, 82^2, 83, 86, 89, 96, 101, 105, 109, 120, 129, 218, 391, 457, 1321, 1560, 2030, 2493, 6036, 6079]
surviving nonsolo ucounts = 10[129, 218, 391, 457, 1321, 1560, 2030, 2493, 6036, 6079]
ids = [662, 10, 35, 494, 218, 161, 768, 77, 330, 6]

====================================================================================

UMI info for barcode AGTGAGGAGTGTGGCA-1 contig 1 = CTGAGCACAG...
umi AAATGGCTAT = 5333 reads: -381 +6 -1XX +1 -1XX +2 -1XX +5 -2XX +1 -1XX +1 invalidated
umi AACAGGCGAT = 183 reads: -398X +2 -1XX +2 invalidated
umi AAGGTTTTAG = 334 reads: -383X +1 -3XX +2 -9XX +2 -1XX +2 invalidated
umi ATCATAGTTA = 1568 reads: +403 validated
umi CAATGTAGTG = 1232 reads: -388 +1 -9X +2 -1XX +2 invalidated
umi CGCTATGATT = 6148 reads: +121 -5XX +2 -6XX +1 -3XX +1 -2XX +1 -200XX +1 -8XX +1 -1XX +1 -1XX +1 -1XX +46 invalidated
umi GGAGATAGCG = 408 reads: -384X +2 -2XX +5 -1XX +1 -2XX +1 -2XX +3 invalidated
umi TCCTGGCCTT = 96 reads: -398X +2 -1XX +2 invalidated
umi TTCTCACATG = 2006 reads: +403 validated

GOOD CONTIGS

TIG 1[bases=492]
0-37 ==> 272-309 on |630|TRBV5-1|5'UTR| [len=309] (mis=0)
37-380 ==> 0-343 on |631|TRBV5-1|L-REGION+V-REGION| [len=343] (mis=0)
392-440 ==> 0-48 on |536|TRBJ1-1|J-REGION| [len=48] (mis=2)
440-492 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 2 umis using 227 reads
cdr3 = CASSLAPGVGDTEAFF at 364, score = 9 + 8
umis assigned: [6, 10, 35, 161, 218, 330, 494, 662, 768]
of which 9 are surviving nonsolos
reads assigned: 17036
start codons at 37, 319, 323
confident = true

REJECT CONTIGS

TIG 1[bases=457]
6-355 ==> 184-533 on segment before TRAJ54 exon 1 [len=533] (mis=0)
355-415 ==> 0-60 on |441|TRAJ54|J-REGION| [len=60] (mis=0)
415-457 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
umis assigned: [77]
of which 1 are surviving nonsolos
reads assigned: 2458
start codons at 58, 67, 110, 153, 192, 289
confident = false
did not find CDR3
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.50 = AGTGAGGAGTTAGCGG-1

using 67 reads

====================================================================================

graph has 66 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[2, 4, 23, 36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.53 = AGTGAGGCAAAGTCAA-1

using 105 reads

====================================================================================

graph has 190 edges initially, 8 edges after simplification

total ucounts = 13
nonsolo ucounts = 9[2^2, 3^2, 4, 5, 16, 21, 45]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.57 = AGTGAGGCAAGACGTG-1

using 9510 reads

====================================================================================

graph has 7716 edges initially, 262 edges after simplification

total ucounts = 1011
nonsolo ucounts = 733[2^113, 3^75, 4^58, 5^54, 6^44, 7^44, 8^35, 9^28, 10^19, 11^20, 12^27, 13^16, 14^16, 15^15, 16^11, 17^12, 18^8, 19^9, 20^11, 21^10, 22^5, 23^5, 24^6, 25^7, 26^5, 27^6, 28^4, 29^3, 30^2, 31^2, 32^5, 33^2, 34^3, 36, 37^2, 38^2, 39, 40^4, 41^3, 42, 43^2, 44^3, 48, 49, 50, 52, 53^3, 54^2, 56, 59, 61, 62^2, 63, 65^2, 66, 68, 69, 70, 76^2, 81, 82, 84, 87, 91, 94, 96, 97, 102, 103, 105]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.59 = AGTGAGGCAAGCCGCT-1

using 104 reads

====================================================================================

graph has 122 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[104]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.60 = AGTGAGGCAAGGACTG-1

using 81 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[81]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.61 = AGTGAGGCAAGGCTCC-1

using 16 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[2, 14]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.66 = AGTGAGGCAATGTAAG-1

using 121 reads

====================================================================================

graph has 104 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[120]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.69 = AGTGAGGCACACTGCG-1

using 16826 reads

====================================================================================

graph has 8330 edges initially, 190 edges after simplification

total ucounts = 1161
nonsolo ucounts = 842[2^172, 3^108, 4^73, 5^59, 6^42, 7^38, 8^34, 9^25, 10^24, 11^15, 12^20, 13^13, 14^10, 15^23, 16^13, 17^11, 18^8, 19^6, 20^6, 21^10, 22^4, 23^5, 24^5, 25^8, 26^9, 27^3, 28^6, 29, 30^5, 31^3, 32^8, 34^2, 35^4, 36^2, 37^2, 38^2, 39^2, 41^3, 42, 43, 44^4, 45, 46^2, 47^4, 48^2, 49, 50, 52, 54^2, 55^2, 56^3, 57^3, 58^2, 59, 61^2, 64^2, 65^2, 66, 67, 69, 72, 73^2, 74, 75, 80, 81, 83, 90, 91, 92, 93, 99, 103, 104, 112, 2204, 4044]
surviving nonsolo ucounts = 2[2204, 4044]
ids = [1048, 828]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.71 = AGTGAGGCACATGACT-1

using 31 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.73 = AGTGAGGCACCATCCT-1

using 2559 reads

====================================================================================

graph has 680 edges initially, 4 edges after simplification

total ucounts = 10
nonsolo ucounts = 5[2, 5, 10, 18, 2519]
surviving nonsolo ucounts = 1[2519]
ids = [2]

====================================================================================

REJECT CONTIGS

TIG 1[bases=417]
13-315 ==> 45-347 on |679|TRBV7-3|L-REGION+V-REGION| [len=347] (mis=1)
315-365 ==> 0-50 on |538|TRBJ1-3|J-REGION| [len=50] (mis=0)
365-417 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
cdr3 = CASSLNSGNTIYF at 298, score = 8 + 7
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 2497
start codons at 77, 191
confident = false
not full
VJ delta = 19
not full
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.75 = AGTGAGGCACCCTATC-1

using 70 reads

====================================================================================

graph has 48 edges initially, 4 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[11, 16, 41]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.77 = AGTGAGGCACCTATCC-1

using 112 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[112]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.80 = AGTGAGGCACGGCGTT-1

using 69 reads

====================================================================================

graph has 80 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 7[2^2, 3, 9, 15, 16, 21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.83 = AGTGAGGCAGAAGCAC-1

using 719 reads

====================================================================================

graph has 278 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 4[2, 3, 25, 689]
surviving nonsolo ucounts = 1[689]
ids = [2]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.87 = AGTGAGGCAGCCTATA-1

using 192 reads

====================================================================================

graph has 126 edges initially, 4 edges after simplification

total ucounts = 13
nonsolo ucounts = 9[2, 4, 5^3, 10, 14, 32, 111]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.91 = AGTGAGGCAGCTGGCT-1

using 78 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[2, 76]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.92 = AGTGAGGCAGTAAGCG-1

using 2245 reads

====================================================================================

graph has 756 edges initially, 4 edges after simplification

total ucounts = 6
nonsolo ucounts = 2[25, 2216]
surviving nonsolo ucounts = 1[2216]
ids = [1]

====================================================================================

REJECT CONTIGS

TIG 1[bases=471]
0-19 ==> 17-36 on |455|TRAV10|5'UTR| [len=36] (mis=0)
19-362 ==> 0-343 on |456|TRAV10|L-REGION+V-REGION| [len=343] (mis=2)
368-429 ==> 0-61 on |427|TRAJ40|J-REGION| [len=61] (mis=2)
429-471 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 2194
start codons at 19, 77, 147, 229, 281
confident = false
frameshifted full length transcript of length 471
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.96 = AGTGAGGCATATACCG-1

using 55 reads

====================================================================================

graph has 70 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 8[2^3, 3, 4, 7, 11, 21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.97 = AGTGAGGCATCAGTCA-1

using 74 reads

====================================================================================

graph has 60 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 3[2, 31, 41]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.99 = AGTGAGGCATCTCCCA-1

using 75 reads

====================================================================================

graph has 82 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[14, 16, 17, 26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.100 = AGTGAGGCATGCAACT-1

using 107 reads

====================================================================================

graph has 167 edges initially, 4 edges after simplification

total ucounts = 10
nonsolo ucounts = 9[4^2, 5^3, 10^2, 16, 47]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.103 = AGTGAGGCATTACGAC-1

using 123 reads

====================================================================================

graph has 116 edges initially, 4 edges after simplification

total ucounts = 18
nonsolo ucounts = 13[2^2, 3, 4^2, 5, 7, 9, 10^2, 11, 15, 36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.105 = AGTGAGGCATTGGCGC-1

using 205 reads

====================================================================================

graph has 212 edges initially, 12 edges after simplification

total ucounts = 17
nonsolo ucounts = 12[3^3, 7^2, 10, 15, 17, 21, 22, 40, 52]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.107 = AGTGAGGCATTTCAGG-1

using 55 reads

====================================================================================

graph has 58 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 5[2, 3, 9, 12, 26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.108 = AGTGAGGCATTTGCTT-1

using 15 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.109 = AGTGAGGGTAATTGGA-1

using 56 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[56]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.111 = AGTGAGGGTAGAAAGG-1

using 51 reads

====================================================================================

graph has 62 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 8[3, 4, 6^3, 7, 8, 10]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.115 = AGTGAGGGTCATATCG-1

using 116 reads

====================================================================================

graph has 142 edges initially, 4 edges after simplification

total ucounts = 12
nonsolo ucounts = 11[2^3, 3, 4^2, 7, 12, 14, 29, 36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.120 = AGTGAGGGTCTTCAAG-1

using 33 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.123 = AGTGAGGGTGATAAAC-1

using 267 reads

====================================================================================

graph has 126 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[266]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.124 = AGTGAGGGTGATGTCT-1

using 61 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[61]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.127 = AGTGAGGGTGTAACGG-1

using 80 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[2, 7, 16, 53]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.128 = AGTGAGGGTGTCAATC-1

using 28 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.129 = AGTGAGGGTGTCCTCT-1

using 511 reads

====================================================================================

graph has 477 edges initially, 18 edges after simplification

total ucounts = 39
nonsolo ucounts = 32[2^3, 3^3, 5, 7^2, 8^2, 9^3, 10, 11, 12^3, 16, 18, 21, 22^2, 27^3, 29, 33, 34^2, 60]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.130 = AGTGAGGGTGTCTGAT-1

using 67 reads

====================================================================================

graph has 58 edges initially, 4 edges after simplification

total ucounts = 5
nonsolo ucounts = 5[2, 5, 16, 17, 27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.138 = AGTGAGGGTTCATGGT-1

using 219 reads

====================================================================================

graph has 206 edges initially, 10 edges after simplification

total ucounts = 16
nonsolo ucounts = 13[2^3, 8, 11, 15, 16^2, 18, 19^2, 42, 46]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.139 = AGTGAGGGTTCCACTC-1

using 135 reads

====================================================================================

graph has 175 edges initially, 4 edges after simplification

total ucounts = 14
nonsolo ucounts = 6[3, 4, 6, 14, 36, 64]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.141 = AGTGAGGGTTCCCTTG-1

using 19 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.142 = AGTGAGGGTTCGCGAC-1

using 1789 reads

====================================================================================

graph has 889 edges initially, 22 edges after simplification

total ucounts = 337
nonsolo ucounts = 195[2^82, 3^43, 4^19, 5^12, 6^8, 7^4, 8^2, 10^2, 11, 12, 13^2, 15, 19, 26^2, 32, 33, 35, 36, 39, 47, 53, 56, 84, 85, 87^2, 90, 95, 112]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.144 = AGTGAGGGTTGACGTT-1

using 8691 reads

====================================================================================

graph has 8045 edges initially, 308 edges after simplification

total ucounts = 920
nonsolo ucounts = 697[2^87, 3^85, 4^47, 5^32, 6^43, 7^30, 8^29, 9^22, 10^33, 11^24, 12^26, 13^19, 14^15, 15^22, 16^14, 17^16, 18^18, 19^11, 20^19, 21^4, 22^11, 23^5, 24^6, 25^8, 26^5, 27^2, 28^8, 29^3, 30^6, 31^2, 32^5, 33^3, 34^4, 36, 37^2, 38^2, 39^2, 40, 42^3, 43^2, 44, 46^2, 47^3, 48^2, 49, 50, 53, 57, 74, 76, 79, 84, 85, 87, 90, 101]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.145 = AGTGAGGGTTGATTGC-1

using 104 reads

====================================================================================

graph has 96 edges initially, 6 edges after simplification

total ucounts = 11
nonsolo ucounts = 7[4, 6, 9, 14, 16, 22, 29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.146 = AGTGAGGGTTGTGGAG-1

using 21 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.148 = AGTGAGGGTTTGCATG-1

using 115 reads

====================================================================================

graph has 112 edges initially, 6 edges after simplification

total ucounts = 15
nonsolo ucounts = 8[2^3, 3, 4, 5, 39, 51]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.152 = AGTGAGGTCAACGCTA-1

using 79 reads

====================================================================================

graph has 88 edges initially, 4 edges after simplification

total ucounts = 10
nonsolo ucounts = 8[2^3, 3, 4, 5, 22, 37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.154 = AGTGAGGTCAATCTCT-1

using 133 reads

====================================================================================

graph has 180 edges initially, 2 edges after simplification

total ucounts = 18
nonsolo ucounts = 16[2^3, 3^4, 4, 8^2, 11^2, 15^2, 16, 25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.157 = AGTGAGGTCACGGTTA-1

using 110 reads

====================================================================================

graph has 80 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[109]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.158 = AGTGAGGTCAGCCTAA-1

using 41 reads

====================================================================================

graph has 4 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[41]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.160 = AGTGAGGTCAGGCAAG-1

using 84 reads

====================================================================================

graph has 96 edges initially, 2 edges after simplification

total ucounts = 12
nonsolo ucounts = 8[2^2, 3, 9^2, 12, 21, 22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.161 = AGTGAGGTCAGTGTTG-1

using 64 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 3[2, 4, 54]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.162 = AGTGAGGTCAGTTGAC-1

using 29 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.163 = AGTGAGGTCAGTTTGG-1

using 27 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.166 = AGTGAGGTCATGGTCA-1

using 21 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.169 = AGTGAGGTCCAGATCA-1

using 53 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[53]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.170 = AGTGAGGTCCAGTAGT-1

using 27 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.171 = AGTGAGGTCCAGTATG-1

using 106 reads

====================================================================================

graph has 68 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 6[2, 5, 7, 12, 32, 44]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.173 = AGTGAGGTCCGAATGT-1

using 148 reads

====================================================================================

graph has 114 edges initially, 4 edges after simplification

total ucounts = 11
nonsolo ucounts = 8[2, 4, 17, 18^2, 24, 30, 32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.174 = AGTGAGGTCCGCTGTT-1

using 70 reads

====================================================================================

graph has 84 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[70]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.176 = AGTGAGGTCCTAGGGC-1

using 87 reads

====================================================================================

graph has 62 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[87]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.179 = AGTGAGGTCGAATCCA-1

using 108 reads

====================================================================================

graph has 170 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 8[2^2, 5^2, 6, 15, 16, 55]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.180 = AGTGAGGTCGAATGGG-1

using 1824 reads

====================================================================================

graph has 708 edges initially, 4 edges after simplification

total ucounts = 13
nonsolo ucounts = 9[2^3, 3, 6, 11, 23, 33, 1738]
surviving nonsolo ucounts = 1[1738]
ids = [0]

====================================================================================

UMI info for barcode AGTGAGGTCGAATGGG-1 contig 1 = GGGAGCCATG...
umi AATATAGGTG = 1741 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=453]
7-351 ==> 0-344 on |613|TRBV28|L-REGION+V-REGION| [len=344] (mis=5)
354-401 ==> 0-47 on |550|TRBJ2-7|J-REGION| [len=47] (mis=2)
401-453 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 1 umis using 104 reads
cdr3 = CASSTTLFYEQYF at 334, score = 6 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 1719
start codons at 7, 65, 129, 142, 149, 157, 212, 215, 223, 325
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.183 = AGTGAGGTCGCCAAAT-1

using 32 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.187 = AGTGAGGTCGGACAAG-1

using 58 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[58]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.189 = AGTGAGGTCGGTTCGG-1

using 42 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[42]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.190 = AGTGAGGTCGTAGGAG-1

using 45 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[45]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.199 = AGTGAGGTCTCTAGGA-1

using 107 reads

====================================================================================

graph has 100 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 3[2, 37, 64]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.200 = AGTGAGGTCTCTTATG-1

using 78 reads

====================================================================================

graph has 104 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 7[2, 6, 8, 9^2, 20, 21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.201 = AGTGAGGTCTGAAAGA-1

using 88 reads

====================================================================================

graph has 99 edges initially, 2 edges after simplification

total ucounts = 16
nonsolo ucounts = 14[2^4, 3^2, 4, 5, 7^2, 8, 10, 15, 16]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.204 = AGTGAGGTCTGGAGCC-1

using 37 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[5, 32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.207 = AGTGAGGTCTGTTTGT-1

using 81 reads

====================================================================================

graph has 90 edges initially, 4 edges after simplification

total ucounts = 10
nonsolo ucounts = 7[2, 3^2, 5, 7, 26, 32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.208 = AGTGAGGTCTTAACCT-1

using 61 reads

====================================================================================

graph has 129 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 6[3^2, 5, 7, 15, 25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.209 = AGTGAGGTCTTACCGC-1

using 149 reads

====================================================================================

graph has 102 edges initially, 2 edges after simplification

total ucounts = 12
nonsolo ucounts = 5[5^2, 12, 17, 103]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.210 = AGTGAGGTCTTCATGT-1

using 62 reads

====================================================================================

graph has 52 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[62]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.213 = AGTGAGGTCTTTAGGG-1

using 26347 reads

====================================================================================

graph has 16801 edges initially, 562 edges after simplification

total ucounts = 2632
nonsolo ucounts = 1932[2^315, 3^199, 4^145, 5^121, 6^95, 7^108, 8^66, 9^46, 10^55, 11^54, 12^41, 13^50, 14^41, 15^45, 16^26, 17^35, 18^34, 19^16, 20^26, 21^27, 22^27, 23^18, 24^31, 25^17, 26^19, 27^12, 28^18, 29^16, 30^17, 31^16, 32^11, 33^12, 34^6, 35^12, 36^8, 37^7, 38^10, 39^5, 40^10, 41^6, 42^6, 43^6, 44^9, 45^4, 46^4, 47^3, 48^3, 49^3, 50^4, 51^2, 52^2, 53^6, 54^3, 55^4, 56^2, 57, 58^2, 59^3, 60, 61, 62^4, 63^3, 64, 65^4, 66^2, 67^3, 68^3, 69, 71, 72, 73^3, 74^2, 76, 79^2, 80^2, 81, 84, 85, 86, 90, 94, 126]
surviving nonsolo ucounts = 3[4, 15, 46]
ids = [752, 2151, 1751]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.215 = AGTGGGAAGAGCAATT-1

using 28 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.219 = AGTGGGAAGATGAGAG-1

using 220 reads

====================================================================================

graph has 102 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 5[3, 9, 16, 17, 172]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.222 = AGTGGGAAGATGTTAG-1

using 1915 reads

====================================================================================

graph has 598 edges initially, 4 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[2, 32, 1879]
surviving nonsolo ucounts = 1[1879]
ids = [2]

====================================================================================

UMI info for barcode AGTGGGAAGATGTTAG-1 contig 1 = ATCTCAGACC...
umi GACCCAGGGC = 1882 reads: +409 validated

GOOD CONTIGS

TIG 1[bases=481]
0-20 ==> 91-111 on |628|TRBV4-3|5'UTR| [len=111] (mis=0)
20-364 ==> 0-344 on |629|TRBV4-3|L-REGION+V-REGION| [len=344] (mis=1)
362-378 ==> 0-16 on |730|TRBD2|D-REGION| [len=16] (mis=3)
380-429 ==> 0-49 on |546|TRBJ2-3|J-REGION| [len=49] (mis=1)
429-481 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 1 umis using 119 reads
cdr3 = CASSQGGSGRPGTDTQYF at 347, score = 10 + 8
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 1854
start codons at 20, 74, 113, 119, 142, 170, 212, 277
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.228 = AGTGGGAAGCGACGTA-1

using 46 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 3[2^2, 39]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.230 = AGTGGGAAGCTAAACA-1

using 427 reads

====================================================================================

graph has 342 edges initially, 10 edges after simplification

total ucounts = 25
nonsolo ucounts = 17[2^3, 3, 5, 7, 8, 12, 20, 25, 29, 32, 36, 48, 56, 62, 70]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.231 = AGTGGGAAGCTAACTC-1

using 2938 reads

====================================================================================

graph has 2171 edges initially, 54 edges after simplification

total ucounts = 295
nonsolo ucounts = 193[2^41, 3^21, 4^14, 5^10, 6^7, 7^7, 8^3, 9^8, 10^4, 11^6, 12^4, 13^5, 14, 15^2, 16^2, 17^2, 18^3, 19^4, 20^3, 21, 23, 24^2, 25^3, 26^4, 27^2, 28^2, 29, 30^2, 33, 35, 37^2, 38^2, 41, 42, 45, 46^3, 47, 48, 49, 50^2, 51, 54, 55^2, 60^2, 64, 65, 67, 73, 77]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.232 = AGTGGGAAGCTAGTGG-1

using 72 reads

====================================================================================

graph has 34 edges initially, 4 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[29, 43]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.233 = AGTGGGAAGCTATGCT-1

using 131 reads

====================================================================================

graph has 182 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 9[2^2, 4, 5, 6, 7, 10, 16, 79]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.234 = AGTGGGAAGCTTTGGT-1

using 19 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.239 = AGTGGGAAGGAGCGAG-1

using 481 reads

====================================================================================

graph has 391 edges initially, 20 edges after simplification

total ucounts = 26
nonsolo ucounts = 19[2^3, 5, 6, 7^3, 9, 13, 20, 22, 39, 45, 48, 49, 50, 69, 72]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.240 = AGTGGGAAGGCCGAAT-1

using 47 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[2, 4, 40]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.242 = AGTGGGAAGGGCTTGA-1

using 56 reads

====================================================================================

graph has 52 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 3[6, 8, 42]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.245 = AGTGGGAAGGTTACCT-1

using 50 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[50]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.246 = AGTGGGAAGTACGACG-1

using 30 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 1[27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.253 = AGTGGGAAGTTGCAGG-1

using 53 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[53]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.255 = AGTGGGACAACACGCC-1

using 24 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 2[8, 13]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.263 = AGTGGGACAAGCTGTT-1

using 46 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[21, 25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.267 = AGTGGGACAATCGGTT-1

using 79 reads

====================================================================================

graph has 108 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 6[2, 9^2, 12, 14, 32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.268 = AGTGGGACAATGACCT-1

using 115 reads

====================================================================================

graph has 88 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 5[2, 3, 5, 14, 88]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.269 = AGTGGGACAATGTTGC-1

using 23 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 3[2, 5, 16]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.275 = AGTGGGACACAGGCCT-1

using 30 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.276 = AGTGGGACACATGACT-1

using 127 reads

====================================================================================

graph has 116 edges initially, 6 edges after simplification

total ucounts = 7
nonsolo ucounts = 7[2, 7, 10, 15, 22, 33, 38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.278 = AGTGGGACACCCAGTG-1

using 67 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[6, 59]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.279 = AGTGGGACACGAAACG-1

using 7330 reads

====================================================================================

graph has 4583 edges initially, 108 edges after simplification

total ucounts = 471
nonsolo ucounts = 322[2^47, 3^37, 4^24, 5^15, 6^25, 7^20, 8^11, 9^12, 10^6, 11^10, 12^7, 13^9, 14^10, 15^5, 16^7, 17^2, 18^3, 19^5, 20^2, 21^5, 22^3, 23^3, 24^3, 25, 26^6, 27^2, 28^2, 29^2, 30^5, 33, 34, 36, 38^2, 40^2, 41, 42^2, 46^2, 47, 49, 50^2, 51, 52^2, 53, 54^2, 60, 61, 64, 65, 68^2, 78, 89, 127, 1230, 1689]
surviving nonsolo ucounts = 2[1230, 1689]
ids = [329, 401]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.286 = AGTGGGACACTTACGA-1

using 42315 reads

====================================================================================

graph has 18070 edges initially, 348 edges after simplification

total ucounts = 1281
nonsolo ucounts = 919[2^161, 3^105, 4^79, 5^56, 6^41, 7^24, 8^36, 9^31, 10^25, 11^22, 12^19, 13^16, 14^23, 15^15, 16^11, 17^14, 18^12, 19^9, 20^12, 21^12, 22^7, 23^14, 24^13, 25, 26^6, 27^8, 28^5, 29^5, 30^5, 31^6, 32^9, 33^2, 34^9, 35^5, 36^2, 37^8, 38^5, 39^6, 40^4, 41^3, 42, 43^4, 44^7, 45^5, 46^2, 47^3, 48, 49, 51^3, 52^2, 53^2, 54^3, 55, 56, 57, 58, 59^2, 60^2, 62, 63^2, 64^2, 67, 71, 72, 76^2, 79, 80, 114, 263, 277, 307, 312, 825, 972, 1046, 1463, 2019, 2174^2, 2244, 2358, 2454, 2611, 2783, 2934, 3022]
surviving nonsolo ucounts = 18[263, 277, 307, 312, 825, 972, 1046, 1463, 2019, 2174^2, 2244, 2358, 2454, 2611, 2783, 2934, 3022]
ids = [108, 413, 152, 44, 1242, 952, 387, 1071, 297, 391, ...]

====================================================================================

UMI info for barcode AGTGGGACACTTACGA-1 contig 1 = AGAATGCTTA...
umi ACATGCGGTT = 171 reads: +381 -6 +4 non-validated
umi ATCCCACGGT = 2473 reads: +391 validated
umi CAGTGCCTCG = 2068 reads: +363 -1XX +1 -3XX +1 -1 +21 invalidated
umi CCCCTGCGCG = 2183 reads: +391 validated
umi GATGACAGCC = 2818 reads: +363 -1XX +1 -2XX +24 invalidated
umi GTATGCTACG = 2598 reads: +391 validated
umi TATAATGGCC = 641 reads: +371 -10 +10 non-validated
umi TCGTCCCAGC = 3041 reads: +391 validated
umi TTGCTATGGT = 2944 reads: +391 validated
umi TTTACGTTCT = 820 reads: +391 validated
umi TTTGTTCACC = 2356 reads: +391 validated

UMI info for barcode AGTGGGACACTTACGA-1 contig 2 = ACAGAAGTGG...
umi ACCGCCTTCG = 2263 reads: +400 validated
umi AGAACTACTA = 264 reads: +400 validated
umi ATAGCAACAC = 270 reads: -367 +5 -3X +6 -3XX +2 -4XX +2 -1XX +1 -2XX +1 -2XX +1 invalidated
umi CCCCCCTCTA = 954 reads: -376 +1 -1XX +2 -1XX +2 -1XX +2 -1XX +1 -3XX +1 -2XX +1 -1XX +2 -2XX invalidated
umi CCCTTTCTGT = 242 reads: -363 +7 -1 +1 -3XX +6 -3XX +2 -4XX +2 -1XX +1 -2XX +1 -2XX +1 invalidated
umi CTCTGCGGTT = 2193 reads: +400 validated
umi TCTCTTGTCT = 1413 reads: +400 validated

GOOD CONTIGS

TIG 1[bases=490]
0-47 ==> 0-47 on |692|TRBV9|5'UTR| [len=47] (mis=0)
47-390 ==> 0-343 on |693|TRBV9|L-REGION+V-REGION| [len=343] (mis=1)
387-438 ==> 0-51 on |544|TRBJ2-2|J-REGION| [len=51] (mis=5)
438-490 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 9 umis using 1652 reads
cdr3 = CASSVRSGELFF at 374, score = 9 + 9
umis assigned: [44, 174, 297, 391, 731, 849, 952, 1060, 1225, 1242] and 1 others
of which 11 are surviving nonsolos
reads assigned: 21641
start codons at 3, 47, 169, 255
confident = true

TIG 2[bases=511]
0-69 ==> 0-69 on |478|TRAV20|5'UTR| [len=69] (mis=0)
69-406 ==> 0-337 on |479|TRAV20|L-REGION+V-REGION| [len=337] (mis=0)
414-469 ==> 8-63 on |444|TRAJ58|J-REGION| [len=63] (mis=0)
469-511 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 4 umis using 328 reads
cdr3 = CAVQGLLTSGSRLTF at 393, score = 8 + 9
umis assigned: [62, 108, 152, 387, 413, 618, 1071]
of which 7 are surviving nonsolos
reads assigned: 7466
start codons at 69, 78
confident = true
now this is a cell
paired!

CCTGAAGACTCAGCCACTTATCTCTGTGCTGTGCAGGGGTTGTTGACCAGTGGCTCTAGGTTGACCTTTGGGGAAGGAACACAGCTCACAGTGAATCCTG <==> AGCTCTCTGGAGCTGGGGGACTCAGCTTTGTATTTCTGTGCCAGCAGCGTAAGGTCCGGGGAGCTGTTTTTTGGAGAAGGCTCTAGGCTGACCGTACTGG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.293 = AGTGGGACAGGTCGTC-1

using 49 reads

====================================================================================

graph has 28 edges initially, 4 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[48]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.298 = AGTGGGACAGTCTTCC-1

using 2782 reads

====================================================================================

graph has 1036 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 3[2, 6, 2770]
surviving nonsolo ucounts = 1[2770]
ids = [3]

====================================================================================

REJECT CONTIGS

TIG 1[bases=429]
13-315 ==> 45-347 on |687|TRBV7-8|L-REGION+V-REGION| [len=347] (mis=0)
326-377 ==> 0-51 on |544|TRBJ2-2|J-REGION| [len=51] (mis=3)
377-429 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
cdr3 = CASSLATSGSYTGELFF at 298, score = 8 + 9
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 2723
start codons at 77, 110, 176
confident = false
not full
VJ delta = 20
not full
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.301 = AGTGGGACAGTGGGAT-1

using 32 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[12, 20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.303 = AGTGGGACATACGCCG-1

using 32 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[2, 4, 8, 16]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.305 = AGTGGGACATCCGGGT-1

using 45 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[45]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.308 = AGTGGGACATCGTCGG-1

using 79 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[2, 76]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.309 = AGTGGGACATGCCCGA-1

using 80 reads

====================================================================================

graph has 59 edges initially, 4 edges after simplification

total ucounts = 7
nonsolo ucounts = 4[7^2, 31, 32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.316 = AGTGGGAGTAAAGTCA-1

using 128 reads

====================================================================================

graph has 104 edges initially, 4 edges after simplification

total ucounts = 14
nonsolo ucounts = 9[2^2, 3^3, 6, 20, 31, 53]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.318 = AGTGGGAGTAAGAGGA-1

using 32 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 1[30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.319 = AGTGGGAGTAAGGGAA-1

using 60 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[2, 58]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.320 = AGTGGGAGTAAGTGGC-1

using 31 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.322 = AGTGGGAGTACCGAGA-1

using 77 reads

====================================================================================

graph has 96 edges initially, 2 edges after simplification

total ucounts = 13
nonsolo ucounts = 9[2, 3^3, 5^3, 14, 33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.325 = AGTGGGAGTACGAAAT-1

using 8183 reads

====================================================================================

graph has 6487 edges initially, 194 edges after simplification

total ucounts = 776
nonsolo ucounts = 541[2^76, 3^54, 4^38, 5^32, 6^21, 7^16, 8^22, 9^16, 10^20, 11^18, 12^16, 13^20, 14^10, 15^13, 16^12, 17^12, 18^7, 19^6, 20^5, 21^5, 22^7, 23^4, 24^6, 25^7, 26^7, 27^4, 28^5, 29^6, 30, 31^6, 32^5, 33^3, 34^5, 35^2, 36, 37^2, 38^3, 39^2, 40^2, 41, 42, 43^3, 44^3, 45^4, 46, 47^3, 48^3, 49, 50, 52, 53^2, 55, 56^2, 58, 60, 62^2, 63, 65, 67, 68^2, 69, 71, 76^2, 77, 79, 82, 90]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.326 = AGTGGGAGTAGAGGAA-1

using 53 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[52]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.327 = AGTGGGAGTAGCGTAG-1

using 11504 reads

====================================================================================

graph has 7065 edges initially, 198 edges after simplification

total ucounts = 681
nonsolo ucounts = 495[2^59, 3^35, 4^41, 5^44, 6^23, 7^18, 8^20, 9^24, 10^15, 11^8, 12^18, 13^16, 14^19, 15^15, 16^9, 17^12, 18^7, 19^7, 20^7, 21^3, 22^5, 23^8, 24^8, 25^8, 26^5, 27^2, 28^2, 29^2, 30, 31^5, 32^5, 33^2, 34^6, 35, 37^4, 38, 39^4, 40^3, 44^4, 46^4, 51^2, 53, 57^2, 58, 60, 61, 70, 79^2, 100, 111, 113, 4652]
surviving nonsolo ucounts = 1[4652]
ids = [393]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.335 = AGTGGGAGTCCAGTTA-1

using 23 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.340 = AGTGGGAGTCGAGTTT-1

using 58 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[58]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.341 = AGTGGGAGTCGCGGTT-1

using 62 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 4[2, 5, 17, 33]
surviving nonsolo ucounts = 2[2, 5]
ids = [4, 2]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.344 = AGTGGGAGTCTCGTTC-1

using 204 reads

====================================================================================

graph has 328 edges initially, 2 edges after simplification

total ucounts = 12
nonsolo ucounts = 8[2, 3^2, 5, 7, 8, 12, 160]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.346 = AGTGGGAGTCTGATCA-1

using 96 reads

====================================================================================

graph has 84 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[96]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.350 = AGTGGGAGTGCAGTAG-1

using 72 reads

====================================================================================

graph has 70 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[35, 37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.352 = AGTGGGAGTGTCCTCT-1

using 286 reads

====================================================================================

graph has 214 edges initially, 6 edges after simplification

total ucounts = 13
nonsolo ucounts = 10[2, 3, 4, 8, 9, 13, 20, 53, 67, 104]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.353 = AGTGGGAGTGTGAATA-1

using 4220 reads

====================================================================================

graph has 1003 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 6[2, 3^2, 11, 13, 4185]
surviving nonsolo ucounts = 1[4185]
ids = [5]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.354 = AGTGGGAGTGTGACGA-1

using 21 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.357 = AGTGGGAGTGTTGGGA-1

using 49 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[49]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.358 = AGTGGGAGTGTTTGTG-1

using 79 reads

====================================================================================

graph has 60 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[35, 43]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.359 = AGTGGGAGTTCCACGG-1

using 24 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.362 = AGTGGGAGTTGGACCC-1

using 45 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[44]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.363 = AGTGGGAGTTGGTGGA-1

using 54 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 2[2, 48]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.366 = AGTGGGATCAACGCTA-1

using 100 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[100]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.371 = AGTGGGATCACGGTTA-1

using 77 reads

====================================================================================

graph has 68 edges initially, 4 edges after simplification

total ucounts = 7
nonsolo ucounts = 5[3^2, 5, 22, 42]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.372 = AGTGGGATCACTTATC-1

using 41 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[41]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.377 = AGTGGGATCATAGCAC-1

using 88 reads

====================================================================================

graph has 134 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 8[2, 3^2, 6, 7, 11, 20, 33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.382 = AGTGGGATCCACTGGG-1

using 25 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.387 = AGTGGGATCCGAATGT-1

using 50 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[7, 43]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.392 = AGTGGGATCCTTGGTC-1

using 326 reads

====================================================================================

graph has 196 edges initially, 20 edges after simplification

total ucounts = 7
nonsolo ucounts = 6[4, 19, 49, 60, 71, 122]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.396 = AGTGGGATCGAGAGCA-1

using 29 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[2^2, 23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.404 = AGTGGGATCGTGGTCG-1

using 16 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[16]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.405 = AGTGGGATCGTTACGA-1

using 24 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[2, 8, 12]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.406 = AGTGGGATCGTTGACA-1

using 38 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.407 = AGTGGGATCGTTGCCT-1

using 45 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[19, 24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.410 = AGTGGGATCTCTAGGA-1

using 65 reads

====================================================================================

graph has 62 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 3[16, 19, 27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.411 = AGTGGGATCTCTGAGA-1

using 66 reads

====================================================================================

graph has 58 edges initially, 4 edges after simplification

total ucounts = 7
nonsolo ucounts = 4[4, 5, 25, 29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.417 = AGTGGGATCTGATTCT-1

using 6516 reads

====================================================================================

graph has 5130 edges initially, 152 edges after simplification

total ucounts = 699
nonsolo ucounts = 447[2^75, 3^49, 4^31, 5^20, 6^25, 7^27, 8^10, 9^15, 10^8, 11^9, 12^18, 13^9, 14^9, 15^11, 16^10, 17^5, 18^6, 19^9, 20^9, 21^5, 22^3, 23^3, 24^6, 25^2, 26^4, 27, 28^4, 29^3, 30^4, 31^3, 32^3, 33, 34, 35, 36^5, 37, 38^3, 39^3, 40, 41^2, 42, 43^4, 44, 45^3, 46^2, 47, 48^2, 50, 52, 54, 56, 57, 59^3, 62, 63, 65, 69, 71, 72, 73, 74, 77, 114, 123]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.419 = AGTGGGATCTGGCGTG-1

using 136 reads

====================================================================================

graph has 70 edges initially, 4 edges after simplification

total ucounts = 7
nonsolo ucounts = 5[2^2, 8, 35, 87]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.423 = AGTGGGATCTTCCTTC-1

using 161 reads

====================================================================================

graph has 86 edges initially, 4 edges after simplification

total ucounts = 11
nonsolo ucounts = 6[5, 9, 22, 32, 35, 53]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.429 = AGTGTCAAGAATTGTG-1

using 31 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.431 = AGTGTCAAGACTAGGC-1

using 641 reads

====================================================================================

graph has 448 edges initially, 20 edges after simplification

total ucounts = 21
nonsolo ucounts = 18[5, 6, 7, 8, 9, 12^2, 16, 19, 35, 47^2, 49, 59, 60, 65, 85, 97]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.432 = AGTGTCAAGAGCAATT-1

using 154 reads

====================================================================================

graph has 132 edges initially, 6 edges after simplification

total ucounts = 13
nonsolo ucounts = 9[2, 3^2, 4, 11, 19, 23, 38, 47]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.438 = AGTGTCAAGCAGCGTA-1

using 33 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[2, 31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.442 = AGTGTCAAGCGTCTAT-1

using 8337 reads

====================================================================================

graph has 7930 edges initially, 214 edges after simplification

total ucounts = 1018
nonsolo ucounts = 768[2^116, 3^81, 4^83, 5^57, 6^42, 7^44, 8^52, 9^31, 10^20, 11^25, 12^26, 13^27, 14^16, 15^21, 16^11, 17^14, 18^8, 19^8, 20^6, 21^7, 22^4, 23^6, 24^6, 25^5, 26^4, 27^8, 28, 30^4, 31, 32^2, 34, 35^2, 36^2, 37, 40^3, 41, 42, 43, 44, 45^2, 46, 49^2, 53, 56, 57, 58, 61, 66, 69, 80, 84, 86, 89, 100, 107, 325]
surviving nonsolo ucounts = 1[325]
ids = [507]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.445 = AGTGTCAAGCTAGCCC-1

using 106 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[106]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.447 = AGTGTCAAGCTGATAA-1

using 32 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.451 = AGTGTCAAGGAGTAGA-1

using 843 reads

====================================================================================

graph has 292 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 3[2, 9, 825]
surviving nonsolo ucounts = 1[825]
ids = [2]

====================================================================================

REJECT CONTIGS

TIG 1[bases=598]
0-35 ==> 8698-8733 on segment before TRBV7-9 exon 1 [len=8733] (mis=0)
35-382 ==> 0-347 on |689|TRBV7-9|L-REGION+V-REGION| [len=347] (mis=2)
535-586 ==> 0-51 on |539|TRBJ1-4|J-REGION| [len=51] (mis=0)
cdr3 = CASSSAGTEAFF at 365, score = 7 + 8
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 796
start codons at 35, 59, 144, 243, 356, 527, 541
confident = false
not full
frameshifted full length stopped transcript of length 598
VJ delta = -137
delta too large
not full
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.455 = AGTGTCAAGGCCCTCA-1

using 43 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 1[40]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.458 = AGTGTCAAGGTGATAT-1

using 68 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[68]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.460 = AGTGTCAAGGTGGGTT-1

using 34 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[3, 9^2, 11]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.462 = AGTGTCAAGTAATCCC-1

using 53 reads

====================================================================================

graph has 74 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 7[2^2, 3, 8^2, 12, 14]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.466 = AGTGTCAAGTCGTTTG-1

using 154 reads

====================================================================================

graph has 76 edges initially, 4 edges after simplification

total ucounts = 8
nonsolo ucounts = 4[10, 13, 40, 87]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.470 = AGTGTCAAGTGTACTC-1

using 711 reads

====================================================================================

graph has 294 edges initially, 2 edges after simplification

total ucounts = 12
nonsolo ucounts = 8[2, 4^2, 5^2, 7, 15, 665]
surviving nonsolo ucounts = 1[665]
ids = [4]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.472 = AGTGTCAAGTGTGAAT-1

using 1388 reads

====================================================================================

graph has 300 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 3[4, 5, 1376]
surviving nonsolo ucounts = 1[1376]
ids = [3]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.475 = AGTGTCAAGTTGTAGA-1

using 63 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[63]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.482 = AGTGTCACAAGCTGTT-1

using 18 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.486 = AGTGTCACACAACGTT-1

using 62 reads

====================================================================================

graph has 84 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 4[2, 3, 20, 34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.487 = AGTGTCACACACCGAC-1

using 55 reads

====================================================================================

graph has 100 edges initially, 2 edges after simplification

total ucounts = 22
nonsolo ucounts = 11[2^2, 3^3, 4^2, 5, 6^3]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.490 = AGTGTCACACGACGAA-1

using 53 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 6[2, 5, 7, 8, 12, 18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.491 = AGTGTCACACGGTGTC-1

using 1943 reads

====================================================================================

graph has 678 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 4[2, 3, 4, 1931]
surviving nonsolo ucounts = 1[1931]
ids = [4]

====================================================================================

UMI info for barcode AGTGTCACACGGTGTC-1 contig 1 = GGGGATGGGT...
umi TACCACGGCT = 1957 reads: +418 validated

GOOD CONTIGS

TIG 1[bases=474]
4-366 ==> 0-362 on |739|TRBV7-7|L-REGION+V-REGION| [len=362] (mis=4)
369-381 ==> 0-12 on |535|TRBD1|D-REGION| [len=12] (mis=3)
374-422 ==> 0-48 on |548|TRBJ2-5|J-REGION| [len=48] (mis=4)
422-474 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 1 umis using 134 reads
cdr3 = CASSLINGQGETQYF at 349, score = 5 + 7
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 1909
start codons at 4, 21, 128, 161, 227, 340
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.498 = AGTGTCACAGAGTGTG-1

using 129 reads

====================================================================================

graph has 58 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[128]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.500 = AGTGTCACAGCCTGTG-1

using 54 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[54]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.502 = AGTGTCACAGCTCCGA-1

using 27 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 3[4, 8, 15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.508 = AGTGTCACAGGGTTAG-1

using 58 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[58]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.509 = AGTGTCACAGTCAGCC-1

using 45 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[5, 15, 24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.511 = AGTGTCACATACTACG-1

using 73 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[73]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.513 = AGTGTCACATAGGATA-1

using 206 reads

====================================================================================

graph has 150 edges initially, 6 edges after simplification

total ucounts = 14
nonsolo ucounts = 12[2, 3, 4^2, 8^2, 11, 12, 13, 22, 57, 60]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.514 = AGTGTCACATATGGTC-1

using 10573 reads

====================================================================================

graph has 4957 edges initially, 116 edges after simplification

total ucounts = 497
nonsolo ucounts = 370[2^47, 3^31, 4^26, 5^24, 6^22, 7^13, 8^16, 9^17, 10^18, 11^15, 12^15, 13^5, 14^13, 15^11, 16^11, 17^11, 18^5, 19^13, 20^5, 21^6, 22^3, 23, 24^4, 25^3, 26^2, 27^3, 28^2, 29^4, 31^4, 32^3, 33, 34^2, 35^2, 37, 43, 53, 57, 58, 63, 69, 72, 286, 1649, 2066, 2259]
surviving nonsolo ucounts = 4[286, 1649, 2066, 2259]
ids = [293, 52, 313, 178]

====================================================================================

UMI info for barcode AGTGTCACATATGGTC-1 contig 1 = AGAATGCTTA...
umi ACCTTACTTA = 1532 reads: -362X +1 -2XX +1 -1XX +1 -3XX +1 -3XX +3 -12XX +1 invalidated
umi CAGTGCGGCT = 1 reads: -388X +1 -2X invalidated
umi CCAGCGTGGG = 2264 reads: +391 validated
umi GGCCTTCCCT = 261 reads: -334 +1 -3X +1 -3X +1 -5X +1 -6X +1 -1XX +1 -2XX +1 -1XX +1 -5XX +23 invalidated
umi GTCCGCAGTG = 2081 reads: +391 validated
umi TCGTTCTCAC = 1 reads: -391X invalidated

GOOD CONTIGS

TIG 1[bases=490]
0-47 ==> 0-47 on |692|TRBV9|5'UTR| [len=47] (mis=0)
47-390 ==> 0-343 on |693|TRBV9|L-REGION+V-REGION| [len=343] (mis=3)
387-438 ==> 0-51 on |544|TRBJ2-2|J-REGION| [len=51] (mis=4)
438-490 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 2 umis using 335 reads
cdr3 = CASSPVPGELFF at 374, score = 9 + 9
umis assigned: [52, 162, 178, 293, 313, 402]
of which 4 are surviving nonsolos
reads assigned: 6034
start codons at 3, 47, 169, 255
confident = true
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.515 = AGTGTCACATCGATGT-1

using 54 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[54]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.517 = AGTGTCACATTATCTC-1

using 32 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.521 = AGTGTCAGTACAGCAG-1

using 94 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[94]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.522 = AGTGTCAGTACCCAAT-1

using 13 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[2, 11]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.523 = AGTGTCAGTACCTACA-1

using 51 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[9, 42]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.526 = AGTGTCAGTAGCAAAT-1

using 40 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[40]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.536 = AGTGTCAGTCTAGAGG-1

using 10115 reads

====================================================================================

graph has 2094 edges initially, 4 edges after simplification

total ucounts = 13
nonsolo ucounts = 4[2, 17, 18, 10069]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================

REJECT CONTIGS

TIG 1[bases=307]
5-78 ==> 727-800 on segment before TRAJ12 exon 1 [len=800] (mis=0)
201-265 ==> 0-64 on |395|TRAJ10|J-REGION| [len=64] (mis=0)
265-307 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
umis assigned: [9]
of which 0 are surviving nonsolos
reads assigned: 9953
start codons at 24, 33, 80
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.537 = AGTGTCAGTCTAGCGC-1

using 47 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[47]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.541 = AGTGTCAGTCTCTCGT-1

using 18 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[4, 12]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.549 = AGTGTCAGTGATGCCC-1

using 60 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[59]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.550 = AGTGTCAGTGCAACGA-1

using 70 reads

====================================================================================

graph has 76 edges initially, 2 edges after simplification

total ucounts = 14
nonsolo ucounts = 11[3^3, 4^2, 6^2, 7, 9, 10, 12]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.553 = AGTGTCAGTGTCTGAT-1

using 2009 reads

====================================================================================

graph has 596 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[2, 2006]
surviving nonsolo ucounts = 1[2006]
ids = [2]

====================================================================================

UMI info for barcode AGTGTCAGTGTCTGAT-1 contig 1 = ATGTTCACAG...
umi TGCTATCGGA = 2010 reads: +403 validated

GOOD CONTIGS

TIG 1[bases=507]
0-52 ==> 7-59 on |567|TRBV12-4|5'UTR| [len=59] (mis=0)
52-399 ==> 0-347 on |568|TRBV12-4|L-REGION+V-REGION| [len=347] (mis=2)
406-455 ==> 0-49 on |546|TRBJ2-3|J-REGION| [len=49] (mis=2)
455-507 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 1 umis using 117 reads
cdr3 = CASSLGLAGTDTQYF at 382, score = 8 + 8
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 1980
start codons at 0, 52, 110, 151, 174, 223, 226, 275, 286, 313, 320
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.558 = AGTGTCAGTTATTCTC-1

using 23 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.560 = AGTGTCAGTTCGAATC-1

using 37 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.562 = AGTGTCAGTTGAGGTG-1

using 856 reads

====================================================================================

graph has 607 edges initially, 16 edges after simplification

total ucounts = 44
nonsolo ucounts = 36[2^5, 3^3, 4^3, 7^2, 8^2, 9, 12, 13^3, 15, 16, 29^2, 31, 38, 39, 40, 41, 46, 51, 55, 58, 65, 86, 88]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.564 = AGTGTCAGTTGTACAC-1

using 33 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.569 = AGTGTCATCAAACAAG-1

using 101 reads

====================================================================================

graph has 78 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[3^2, 19, 74]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.570 = AGTGTCATCAACGGCC-1

using 29 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.571 = AGTGTCATCAACGGGA-1

using 35 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.576 = AGTGTCATCAGCCTAA-1

using 43107 reads

====================================================================================

graph has 14104 edges initially, 223 edges after simplification

total ucounts = 677
nonsolo ucounts = 492[2^60, 3^32, 4^27, 5^24, 6^22, 7^17, 8^22, 9^12, 10^22, 11^24, 12^20, 13^19, 14^17, 15^17, 16^9, 17^10, 18^15, 19^9, 20^10, 21^6, 22^10, 23^7, 24^5, 25^5, 26^5, 27^3, 28^2, 29^3, 30^5, 32, 33^3, 34, 35, 36^2, 37, 38, 39, 41, 43, 44, 45^2, 47, 51, 52, 53, 57, 59, 63, 65, 70, 72, 74, 75, 77, 88, 109, 119, 756, 811, 849, 872, 917, 918, 1163, 1203, 1239, 1753, 1893, 1899, 2007, 2155, 2233, 2345, 2463, 2477, 2521, 2712, 3347]
surviving nonsolo ucounts = 22[65, 756, 811, 849, 872, 917, 918, 1163, 1203, 1239, 1753, 1893, 1899, 2007, 2155, 2233, 2345, 2463, 2477, 2521, 2712, 3347]
ids = [238, 21, 124, 275, 443, 324, 501, 42, 149, 583, ...]

====================================================================================

UMI info for barcode AGTGTCATCAGCCTAA-1 contig 1 = TTTTGAAACC...
umi AACTGTATGG = 702 reads: +356 -18 +2 -1 +3 -1 +22 non-validated
umi AGGTTCATTG = 626 reads: +369 -17 +17 non-validated
umi CCATGTGTGC = 61 reads: +156 -28 +2 -1 +96 -120 non-validated
umi CTACATGGGT = 805 reads: +403 validated
umi GTCACCCAGA = 807 reads: +403 validated
umi TATTTGGCCT = 891 reads: +403 validated

UMI info for barcode AGTGTCATCAGCCTAA-1 contig 2 = GAGCACAGAC...
umi AATTTTAGCA = 1171 reads: +397 validated
umi ATCTGGCTTT = 1209 reads: +397 validated
umi ATGATACGGC = 2550 reads: +397 validated
umi ATGCTCCTCC = 2477 reads: +397 validated
umi CCCGTCTGAG = 1897 reads: +397 validated
umi CGCTAAAGGT = 2000 reads: +397 validated
umi CTACGCTCGG = 2148 reads: +397 validated
umi GGATACTCAC = 2481 reads: +397 validated
umi GTACTACTTA = 2245 reads: +397 validated
umi TCTCACCCTT = 2708 reads: +397 validated
umi TGCCCTCTCG = 2354 reads: +397 validated
umi TGCCTCCCTC = 1239 reads: +397 validated
umi TTGACCTTGA = 1768 reads: +397 validated

GOOD CONTIGS

TIG 1[bases=573]
0-128 ==> 0-128 on |523|TRAV8-4|5'UTR| [len=128] (mis=0)
128-469 ==> 0-341 on |524|TRAV8-4|L-REGION+V-REGION| [len=341] (mis=0)
479-531 ==> 9-61 on |427|TRAJ40|J-REGION| [len=61] (mis=0)
531-573 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 3 umis using 45 reads
cdr3 = CAVSEESAGTYKYIF at 455, score = 7 + 8
umis assigned: [21, 124, 238, 324, 443, 501]
of which 6 are surviving nonsolos
reads assigned: 3817
start codons at 128, 291, 431
confident = true

TIG 2[bases=484]
0-35 ==> 274-309 on |630|TRBV5-1|5'UTR| [len=309] (mis=0)
35-378 ==> 0-343 on |631|TRBV5-1|L-REGION+V-REGION| [len=343] (mis=0)
377-389 ==> 0-12 on |535|TRBD1|D-REGION| [len=12] (mis=3)
384-432 ==> 0-48 on |537|TRBJ1-2|J-REGION| [len=48] (mis=5)
432-484 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 13 umis using 1860 reads
cdr3 = CASSLESGRHGYTF at 362, score = 9 + 9
umis assigned: [42, 149, 155, 160, 249, 305, 327, 410, 436, 557] and 3 others
of which 13 are surviving nonsolos
reads assigned: 25764
start codons at 35, 317, 321, 390
confident = true

REJECT CONTIGS

TIG 1[bases=478]
0-36 ==> 31-67 on |614|TRBV29-1|5'UTR| [len=67] (mis=0)
0-36 ==> 5964-6000 on segment before TRBV29OR9-2 exon 1 [len=6000] (mis=0)
36-371 ==> 0-335 on |615|TRBV29-1|L-REGION+V-REGION| [len=335] (mis=2)
368-384 ==> 0-16 on |730|TRBD2|D-REGION| [len=16] (mis=3)
379-426 ==> 0-47 on |550|TRBJ2-7|J-REGION| [len=47] (mis=5)
426-478 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
umis assigned: [57, 275, 542]
of which 3 are surviving nonsolos
reads assigned: 5992
start codons at 36, 171, 174, 250, 327
confident = false
did not find CDR3
now this is a cell
paired!

ATGAGCGACGCGGCTGAGTACTTCTGTGCTGTGAGTGAGGAATCGGCCGGAACCTACAAATACATCTTTGGAACAGGCACCAGGCTGAAGGTTTTAGCAA <==> TTGGAGCTGGGGGACTCGGCCCTTTATCTTTGCGCCAGCAGCTTGGAGTCAGGGCGCCATGGCTACACCTTCGGTTCGGGGACCAGGTTAACCGTTGTAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.578 = AGTGTCATCAGGCGAA-1

using 20 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[2, 16]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.579 = AGTGTCATCAGTACGT-1

using 34 reads

====================================================================================

graph has 48 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[2, 9, 10, 11]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.582 = AGTGTCATCATGCAAC-1

using 58 reads

====================================================================================

graph has 60 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 5[2, 5, 11, 12, 26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.583 = AGTGTCATCATTTGGG-1

using 53 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[53]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.585 = AGTGTCATCCAGTATG-1

using 46 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[3, 13, 14^2]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.586 = AGTGTCATCCCAAGAT-1

using 74 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[73]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.589 = AGTGTCATCCGAATGT-1

using 118 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[118]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.590 = AGTGTCATCCGAGCCA-1

using 31 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.593 = AGTGTCATCCTTGCCA-1

using 114 reads

====================================================================================

graph has 72 edges initially, 4 edges after simplification

total ucounts = 4
nonsolo ucounts = 4[2, 9, 31, 72]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.595 = AGTGTCATCGCACTCT-1

using 267 reads

====================================================================================

graph has 204 edges initially, 12 edges after simplification

total ucounts = 19
nonsolo ucounts = 14[3^4, 7^2, 9^2, 17, 25, 35, 36, 43, 62]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.600 = AGTGTCATCTAGAGTC-1

using 115 reads

====================================================================================

graph has 103 edges initially, 4 edges after simplification

total ucounts = 7
nonsolo ucounts = 7[2, 5, 9, 10, 25, 30, 34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.601 = AGTGTCATCTATCCCG-1

using 43 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 4[2^3, 37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.609 = AGTGTCATCTGGAGCC-1

using 28 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.611 = AGTGTCATCTTCATGT-1

using 52 reads

====================================================================================

graph has 68 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 6[2^2, 5, 11, 12, 19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.613 = AGTGTCATCTTTACGT-1

using 86 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[86]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.614 = AGTTGGTAGACAGGCT-1

using 20 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 3[2^2, 13]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.615 = AGTTGGTAGACCTAGG-1

using 62 reads

====================================================================================

graph has 60 edges initially, 2 edges after simplification

total ucounts = 12
nonsolo ucounts = 7[2, 3^2, 4^2, 17, 24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.617 = AGTTGGTAGATATGCA-1

using 104 reads

====================================================================================

graph has 142 edges initially, 4 edges after simplification

total ucounts = 10
nonsolo ucounts = 10[2^2, 5^2, 6, 9, 10, 17, 22, 26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.618 = AGTTGGTAGATCCCGC-1

using 13 reads

====================================================================================

graph has 6 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[13]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.619 = AGTTGGTAGATTACCC-1

using 49 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[7, 10, 30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.621 = AGTTGGTAGCCACGCT-1

using 85 reads

====================================================================================

graph has 50 edges initially, 4 edges after simplification

total ucounts = 6
nonsolo ucounts = 6[2, 3, 6, 15, 28, 31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.622 = AGTTGGTAGCCGGTAA-1

using 28 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.623 = AGTTGGTAGCGAAGGG-1

using 44 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[44]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.628 = AGTTGGTAGCGTTGCC-1

using 51 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[51]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.629 = AGTTGGTAGCTAGTCT-1

using 11 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[11]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.630 = AGTTGGTAGCTCCTTC-1

using 400 reads

====================================================================================

graph has 236 edges initially, 6 edges after simplification

total ucounts = 12
nonsolo ucounts = 11[2, 6, 7, 8, 25, 41, 49, 54, 60, 69, 78]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.632 = AGTTGGTAGCTTCGCG-1

using 98 reads

====================================================================================

graph has 72 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[98]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.635 = AGTTGGTAGGATGTAT-1

using 32 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.639 = AGTTGGTAGGCGTACA-1

using 60 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[60]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.640 = AGTTGGTAGGCTAGGT-1

using 35 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.644 = AGTTGGTAGGTGTTAA-1

using 236 reads

====================================================================================

graph has 138 edges initially, 4 edges after simplification

total ucounts = 18
nonsolo ucounts = 13[2^2, 3, 6, 10, 13, 15, 17, 23, 29, 30, 32, 49]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.645 = AGTTGGTAGTCGTTTG-1

using 23 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.647 = AGTTGGTAGTGGTCCC-1

using 38 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.649 = AGTTGGTAGTTTAGGA-1

using 36 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.651 = AGTTGGTCAAATTGCC-1

using 23 reads

====================================================================================

graph has 20 edges initially, 4 edges after simplification

total ucounts = 5
nonsolo ucounts = 2[9, 11]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.654 = AGTTGGTCAAGTACCT-1

using 189 reads

====================================================================================

graph has 182 edges initially, 4 edges after simplification

total ucounts = 13
nonsolo ucounts = 12[2, 3, 4, 5^2, 11, 12, 13, 15, 19, 22, 77]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.655 = AGTTGGTCAAGTTGTC-1

using 64 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[64]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.658 = AGTTGGTCAATTCCTT-1

using 57 reads

====================================================================================

graph has 66 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 7[2^2, 3, 5^2, 10, 28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.659 = AGTTGGTCACACTGCG-1

using 54 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[54]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.663 = AGTTGGTCACATCCGG-1

using 85 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 12
nonsolo ucounts = 9[2^2, 3^2, 5, 10, 13, 22^2]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.664 = AGTTGGTCACATGACT-1

using 61 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[61]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.670 = AGTTGGTCACTGTTAG-1

using 21 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[4, 15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.672 = AGTTGGTCAGATCCAT-1

using 66 reads

====================================================================================

graph has 4 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[66]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.674 = AGTTGGTCAGCAGTTT-1

using 28 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.678 = AGTTGGTCAGGTGCCT-1

using 1530 reads

====================================================================================

graph has 1166 edges initially, 26 edges after simplification

total ucounts = 242
nonsolo ucounts = 158[2^33, 3^26, 4^25, 5^14, 6^6, 7^2, 8^9, 9^5, 10^2, 11^4, 12^3, 13^3, 14^2, 15, 16^2, 17, 18^2, 19, 20^3, 21^2, 22^3, 25^2, 37, 43, 71, 72, 75, 77, 86]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.680 = AGTTGGTCAGTCAGCC-1

using 312 reads

====================================================================================

graph has 214 edges initially, 10 edges after simplification

total ucounts = 21
nonsolo ucounts = 13[2^2, 3, 4, 9, 12, 16, 22, 24^2, 44, 49, 93]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.682 = AGTTGGTCATAGGATA-1

using 64 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[64]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.685 = AGTTGGTCATGGAATA-1

using 86 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[85]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.686 = AGTTGGTCATTAACCG-1

using 231 reads

====================================================================================

graph has 200 edges initially, 6 edges after simplification

total ucounts = 17
nonsolo ucounts = 15[3^2, 4^2, 5, 8, 9, 11^3, 12, 13^2, 49, 73]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.689 = AGTTGGTCATTCTTAC-1

using 35 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.690 = AGTTGGTCATTGAGCT-1

using 26 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.694 = AGTTGGTGTAATCACC-1

using 116 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[116]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.696 = AGTTGGTGTACCGTAT-1

using 115 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[114]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.698 = AGTTGGTGTAGAAAGG-1

using 18 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.700 = AGTTGGTGTAGCGTGA-1

using 38 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.701 = AGTTGGTGTAGCTAAA-1

using 91 reads

====================================================================================

graph has 118 edges initially, 2 edges after simplification

total ucounts = 12
nonsolo ucounts = 7[2, 3, 4, 5, 18, 22, 32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.703 = AGTTGGTGTCAATGTC-1

using 37 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[2, 3, 4, 26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.707 = AGTTGGTGTCCAGTGC-1

using 73 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[73]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.715 = AGTTGGTGTGCAACTT-1

using 251 reads

====================================================================================

graph has 206 edges initially, 6 edges after simplification

total ucounts = 25
nonsolo ucounts = 21[2^7, 3^3, 5^2, 6, 8, 11^2, 15, 18, 30, 57, 58]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.718 = AGTTGGTGTGCTCTTC-1

using 3403 reads

====================================================================================

graph has 1100 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 1[3397]
surviving nonsolo ucounts = 1[3397]
ids = [2]

====================================================================================

UMI info for barcode AGTTGGTGTGCTCTTC-1 contig 1 = GGGGACTAAA...
umi AACCCCCGCC = 3422 reads: +400 validated

GOOD CONTIGS

TIG 1[bases=453]
11-349 ==> 0-338 on |530|TRAV9-2|L-REGION+V-REGION| [len=338] (mis=1)
353-411 ==> 8-66 on |413|TRAJ28|J-REGION| [len=66] (mis=0)
411-453 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 1 umis using 214 reads
cdr3 = CALSDLGAGSYQLTF at 335, score = 9 + 10
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 3351
start codons at 11, 86, 174, 228
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.719 = AGTTGGTGTGGAAAGA-1

using 167 reads

====================================================================================

graph has 94 edges initially, 6 edges after simplification

total ucounts = 10
nonsolo ucounts = 8[2, 4, 8, 17, 29, 34, 35, 36]
surviving nonsolo ucounts = 1[4]
ids = [0]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.720 = AGTTGGTGTGGGTCAA-1

using 67 reads

====================================================================================

graph has 126 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 5[2, 4, 5^2, 48]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.721 = AGTTGGTGTGTCAATC-1

using 75 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[75]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.722 = AGTTGGTGTGTCCTCT-1

using 33 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.726 = AGTTGGTGTGTGCCTG-1

using 84 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[83]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.727 = AGTTGGTGTGTTTGGT-1

using 155 reads

====================================================================================

graph has 114 edges initially, 4 edges after simplification

total ucounts = 6
nonsolo ucounts = 5[2^2, 32, 35, 83]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.729 = AGTTGGTGTTAAGTAG-1

using 149 reads

====================================================================================

graph has 138 edges initially, 8 edges after simplification

total ucounts = 12
nonsolo ucounts = 8[2, 5, 8, 11, 17, 28, 31, 43]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.730 = AGTTGGTGTTACGTCA-1

using 109 reads

====================================================================================

graph has 164 edges initially, 4 edges after simplification

total ucounts = 9
nonsolo ucounts = 7[2^2, 3, 7, 9, 23, 61]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.731 = AGTTGGTGTTATGCGT-1

using 147 reads

====================================================================================

graph has 126 edges initially, 4 edges after simplification

total ucounts = 13
nonsolo ucounts = 8[4^2, 5^2, 14, 26, 35, 49]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.734 = AGTTGGTGTTCCTCCA-1

using 202 reads

====================================================================================

graph has 130 edges initially, 8 edges after simplification

total ucounts = 9
nonsolo ucounts = 9[2, 4, 7, 18, 23, 26, 27, 28, 67]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.735 = AGTTGGTGTTCTCATT-1

using 76 reads

====================================================================================

graph has 94 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[76]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.737 = AGTTGGTTCAAACGGG-1

using 36 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.738 = AGTTGGTTCAAAGTAG-1

using 47 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 7[2, 3^2, 4^2, 7, 22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.740 = AGTTGGTTCAACGCTA-1

using 1535 reads

====================================================================================

graph has 504 edges initially, 6 edges after simplification

total ucounts = 10
nonsolo ucounts = 7[2, 4, 9, 21, 28, 42, 1426]
surviving nonsolo ucounts = 1[1426]
ids = [8]

====================================================================================

UMI info for barcode AGTTGGTTCAACGCTA-1 contig 1 = TTCCTCTGCT...
umi TTGCCTGCTT = 1418 reads: +385 validated

GOOD CONTIGS

TIG 1[bases=549]
0-112 ==> 0-112 on |618|TRBV30|5'UTR| [len=112] (mis=0)
112-447 ==> 0-335 on |619|TRBV30|L-REGION+V-REGION| [len=335] (mis=2)
449-497 ==> 0-48 on |536|TRBJ1-1|J-REGION| [len=48] (mis=2)
497-549 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 1 umis using 54 reads
cdr3 = CAWIGQDTEAFF at 433, score = 7 + 8
umis assigned: [8]
of which 1 are surviving nonsolos
reads assigned: 1397
start codons at 109, 112, 180
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.742 = AGTTGGTTCACAGTAC-1

using 71 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[71]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.745 = AGTTGGTTCACCAGGC-1

using 1272 reads

====================================================================================

graph has 530 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 1[1269]
surviving nonsolo ucounts = 1[1269]
ids = [3]

====================================================================================

UMI info for barcode AGTTGGTTCACCAGGC-1 contig 1 = ATCCTGCCCT...
umi TTAGCCGCAA = 1277 reads: +400 validated

GOOD CONTIGS

TIG 1[bases=471]
0-19 ==> 26-45 on |563|TRBV11-3|5'UTR| [len=45] (mis=0)
19-366 ==> 0-347 on |564|TRBV11-3|L-REGION+V-REGION| [len=347] (mis=3)
371-419 ==> 0-48 on |548|TRBJ2-5|J-REGION| [len=48] (mis=4)
419-471 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 1 umis using 73 reads
cdr3 = CASSSQGRGETQYF at 349, score = 9 + 7
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 1245
start codons at 19, 221, 227
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.747 = AGTTGGTTCAGATAAG-1

using 107 reads

====================================================================================

graph has 52 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[107]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.750 = AGTTGGTTCATAGCAC-1

using 27 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.756 = AGTTGGTTCCCGGATG-1

using 123 reads

====================================================================================

graph has 94 edges initially, 4 edges after simplification

total ucounts = 9
nonsolo ucounts = 8[2, 3, 5, 6, 11, 14, 38, 43]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.758 = AGTTGGTTCCTCGCAT-1

using 82 reads

====================================================================================

graph has 52 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[81]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.761 = AGTTGGTTCGCACTCT-1

using 34 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.762 = AGTTGGTTCGCCTGAG-1

using 89 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[89]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.763 = AGTTGGTTCGCGCCAA-1

using 65 reads

====================================================================================

graph has 101 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[2, 29, 32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.764 = AGTTGGTTCGGTGTCG-1

using 51 reads

====================================================================================

graph has 54 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[50]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.767 = AGTTGGTTCGTGGTCG-1

using 90 reads

====================================================================================

graph has 90 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 5[3, 5, 8, 30, 41]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.768 = AGTTGGTTCTAAGCCA-1

using 50 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 4[2, 3, 12, 29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.771 = AGTTGGTTCTCGCTTG-1

using 112 reads

====================================================================================

graph has 52 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[112]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.774 = AGTTGGTTCTGGCGAC-1

using 10 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[2, 7]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.776 = AGTTGGTTCTTCAACT-1

using 52 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[52]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.777 = AGTTGGTTCTTCCTTC-1

using 80 reads

====================================================================================

graph has 46 edges initially, 4 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[35, 45]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.779 = AGTTGGTTCTTGTATC-1

using 92 reads

====================================================================================

graph has 100 edges initially, 2 edges after simplification

total ucounts = 12
nonsolo ucounts = 10[2, 3^2, 6^2, 7, 8, 11, 16, 28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.780 = AGTTGGTTCTTTCCTC-1

using 43 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[43]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.781 = ATAACGCAGAAACCGC-1

using 46 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[46]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.782 = ATAACGCAGAAGAAGC-1

using 59 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[5, 9, 44]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.786 = ATAACGCAGAGCCTAG-1

using 79 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[79]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.789 = ATAACGCAGATCGATA-1

using 93 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[93]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.791 = ATAACGCAGCACCGCT-1

using 38 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[15, 23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.795 = ATAACGCAGCGCTCCA-1

using 215 reads

====================================================================================

graph has 170 edges initially, 6 edges after simplification

total ucounts = 12
nonsolo ucounts = 8[3, 5, 7, 16, 17, 33, 38, 92]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.797 = ATAACGCAGCTGATAA-1

using 97 reads

====================================================================================

graph has 80 edges initially, 2 edges after simplification

total ucounts = 24
nonsolo ucounts = 12[2^2, 3^2, 4^2, 6^2, 7, 8, 15, 25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.798 = ATAACGCAGCTTCGCG-1

using 22035 reads

====================================================================================

graph has 7480 edges initially, 80 edges after simplification

total ucounts = 532
nonsolo ucounts = 352[2^69, 3^47, 4^37, 5^31, 6^18, 7^13, 8^14, 9^15, 10^8, 11^8, 12^10, 13^6, 14^6, 15^7, 16^8, 17^4, 18^4, 19, 20^5, 21^7, 22^3, 23, 24, 25^2, 26^2, 27, 28, 32^2, 33^2, 34, 36^2, 39, 40^2, 41, 45, 48, 50, 56, 61, 67, 76, 77, 2461, 4132, 4134, 7865]
surviving nonsolo ucounts = 4[2461, 4132, 4134, 7865]
ids = [233, 411, 141, 188]

====================================================================================

REJECT CONTIGS

TIG 1[bases=346]
6-238 ==> 450-682 on segment before TRAJ32 exon 1 [len=682] (mis=0)
238-304 ==> 0-66 on |418|TRAJ32|J-REGION| [len=66] (mis=0)
304-346 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
umis assigned: [188, 233]
of which 2 are surviving nonsolos
reads assigned: 10102
start codons at 54, 65, 95, 110, 244
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.802 = ATAACGCAGGCATTGG-1

using 73 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[73]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.803 = ATAACGCAGGGAAACA-1

using 40 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[39]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.804 = ATAACGCAGGGCTTCC-1

using 21 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.806 = ATAACGCAGGTGATTA-1

using 45 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[45]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.807 = ATAACGCAGGTGTTAA-1

using 34 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[12, 22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.808 = ATAACGCAGTAACCCT-1

using 50 reads

====================================================================================

graph has 92 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 4[3^2, 6, 34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.811 = ATAACGCAGTGAAGTT-1

using 43 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[43]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.814 = ATAACGCAGTGTACGG-1

using 203 reads

====================================================================================

graph has 202 edges initially, 6 edges after simplification

total ucounts = 15
nonsolo ucounts = 12[2^3, 3, 4, 8, 9, 12, 14, 36, 47, 61]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.818 = ATAACGCCAAGAAGAG-1

using 75 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[75]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.819 = ATAACGCCAAGCCGCT-1

using 72 reads

====================================================================================

graph has 78 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[72]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.822 = ATAACGCCAAGCTGGA-1

using 25 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.826 = ATAACGCCACATCTTT-1

using 82 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[82]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.827 = ATAACGCCACATGGGA-1

using 51 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[51]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.828 = ATAACGCCACCAGTTA-1

using 38 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.832 = ATAACGCCACGACTCG-1

using 2044 reads

====================================================================================

graph has 680 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[2043]
surviving nonsolo ucounts = 1[2043]
ids = [1]

====================================================================================

UMI info for barcode ATAACGCCACGACTCG-1 contig 1 = GAGCACAGAC...
umi ACCAGTTCTT = 2056 reads: +403 validated

GOOD CONTIGS

TIG 1[bases=490]
0-35 ==> 274-309 on |630|TRBV5-1|5'UTR| [len=309] (mis=0)
35-378 ==> 0-343 on |631|TRBV5-1|L-REGION+V-REGION| [len=343] (mis=2)
388-438 ==> 0-50 on |543|TRBJ2-1|J-REGION| [len=50] (mis=1)
438-490 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 1 umis using 103 reads
cdr3 = CASSSRQASSYNEQFF at 362, score = 9 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 2013
start codons at 35, 317, 321, 396
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.839 = ATAACGCCAGACGCAA-1

using 70 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[70]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.847 = ATAACGCCAGGTGGAT-1

using 27 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.851 = ATAACGCCAGTATGCT-1

using 65 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[65]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.853 = ATAACGCCAGTGACAG-1

using 326 reads

====================================================================================

graph has 251 edges initially, 12 edges after simplification

total ucounts = 20
nonsolo ucounts = 17[2^2, 3^3, 4, 5^2, 6, 10, 11^2, 14, 21, 67, 69, 87]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.854 = ATAACGCCAGTGGGAT-1

using 34 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.855 = ATAACGCCATAACCTG-1

using 103 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[103]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.856 = ATAACGCCATAAGACA-1

using 71 reads

====================================================================================

graph has 48 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[5, 65]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.860 = ATAACGCCATCGATGT-1

using 33 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[2, 29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.861 = ATAACGCCATCTGGTA-1

using 74 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 6[2, 3, 5, 6, 11, 46]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.868 = ATAACGCGTACAGACG-1

using 222 reads

====================================================================================

graph has 190 edges initially, 4 edges after simplification

total ucounts = 19
nonsolo ucounts = 14[3, 5^3, 7, 8^2, 11^2, 16, 22, 33, 40, 43]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.869 = ATAACGCGTACAGTTC-1

using 67 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[67]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.871 = ATAACGCGTAGTACCT-1

using 138 reads

====================================================================================

graph has 180 edges initially, 4 edges after simplification

total ucounts = 20
nonsolo ucounts = 10[2, 3, 4, 7, 12^2, 18, 19, 22, 29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.874 = ATAACGCGTCAGAGGT-1

using 65 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[65]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.883 = ATAACGCGTGCGATAG-1

using 46 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 1[43]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.885 = ATAACGCGTGGGTCAA-1

using 149 reads

====================================================================================

graph has 216 edges initially, 4 edges after simplification

total ucounts = 14
nonsolo ucounts = 11[2, 5^2, 9^2, 12, 15, 19^2, 21, 30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.892 = ATAACGCGTTGGTGGA-1

using 262 reads

====================================================================================

graph has 210 edges initially, 4 edges after simplification

total ucounts = 19
nonsolo ucounts = 15[2^3, 3, 5, 6, 7, 15^2, 16, 21, 26, 38, 42, 58]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.893 = ATAACGCGTTTAGCTG-1

using 412 reads

====================================================================================

graph has 344 edges initially, 14 edges after simplification

total ucounts = 24
nonsolo ucounts = 16[2^2, 4, 5, 6, 7, 8, 9, 14, 18, 27, 33, 45, 50, 79, 95]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.894 = ATAACGCTCAACACGT-1

using 3261 reads

====================================================================================

graph has 2253 edges initially, 62 edges after simplification

total ucounts = 297
nonsolo ucounts = 171[2^27, 3^26, 4^12, 5^15, 6^5, 7^12, 8^8, 9^2, 10^2, 11^10, 12^5, 13, 14^4, 15^3, 16^3, 17^3, 18^4, 19, 20, 21^3, 23^3, 26, 27, 28^2, 29, 31^2, 32^4, 34^2, 35, 38, 41, 42, 56, 71, 81, 1278]
surviving nonsolo ucounts = 2[81, 1278]
ids = [87, 281]

====================================================================================

REJECT CONTIGS

TIG 1[bases=555]
2-117 ==> 2460-2575 on segment before TRBJ2-1 exon 1 [len=3226] (mis=0)
167-239 ==> 0-72 on segment before TRBJ2-6 exon 1 [len=72] (mis=0)
239-292 ==> 0-53 on |549|TRBJ2-6|J-REGION| [len=53] (mis=0)
292-456 ==> 0-164 on segment before TRBJ2-7 exon 1 [len=164] (mis=0)
503-555 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
umis assigned: [87, 281]
of which 2 are surviving nonsolos
reads assigned: 1325
start codons at 92, 435
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.895 = ATAACGCTCAACACTG-1

using 60 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[60]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.902 = ATAACGCTCAGGCAAG-1

using 63 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[63]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.903 = ATAACGCTCATGGTCA-1

using 92 reads

====================================================================================

graph has 32 edges initially, 4 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[35, 57]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.904 = ATAACGCTCATGTCTT-1

using 111 reads

====================================================================================

graph has 42 edges initially, 4 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[50, 60]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.905 = ATAACGCTCATTATCC-1

using 240 reads

====================================================================================

graph has 232 edges initially, 6 edges after simplification

total ucounts = 28
nonsolo ucounts = 21[2^5, 3^4, 4^2, 5^2, 8, 9, 14^2, 16, 17, 51, 64]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.906 = ATAACGCTCATTGCCC-1

using 58 reads

====================================================================================

graph has 52 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 6[2^2, 3^2, 6, 40]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.908 = ATAACGCTCCATTCTA-1

using 49 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[49]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.912 = ATAACGCTCCTTCAAT-1

using 50 reads

====================================================================================

graph has 64 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 4[9, 10^2, 15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.913 = ATAACGCTCCTTGGTC-1

using 36 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.914 = ATAACGCTCGGACAAG-1

using 103 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[103]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.916 = ATAACGCTCTAACTCT-1

using 67 reads

====================================================================================

graph has 68 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 6[3^2, 4^2, 8, 41]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.918 = ATAACGCTCTACTCAT-1

using 108 reads

====================================================================================

graph has 134 edges initially, 4 edges after simplification

total ucounts = 10
nonsolo ucounts = 6[2^2, 5, 13, 33, 49]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.919 = ATAACGCTCTCCAACC-1

using 79 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[79]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.920 = ATAACGCTCTCTGAGA-1

using 67 reads

====================================================================================

graph has 6 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[67]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.921 = ATAACGCTCTGATTCT-1

using 50 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[9, 41]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.922 = ATAACGCTCTGCGGCA-1

using 1719 reads

====================================================================================

graph has 754 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 2[6, 1710]
surviving nonsolo ucounts = 1[1710]
ids = [0]

====================================================================================

UMI info for barcode ATAACGCTCTGCGGCA-1 contig 1 = AGAATGACGC...
umi ACATACCTAT = 1702 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=526]
0-80 ==> 0-80 on |651|TRBV6-2|5'UTR| [len=80] (mis=0)
80-424 ==> 0-344 on |652|TRBV6-2|L-REGION+V-REGION| [len=344] (mis=3)
424-474 ==> 0-50 on |543|TRBJ2-1|J-REGION| [len=50] (mis=4)
474-526 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 1 umis using 89 reads
cdr3 = CASSEADHNEQFF at 407, score = 9 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 1674
start codons at 3, 38, 80, 138, 191, 215, 222, 230, 257, 321, 330, 432
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.923 = ATAACGCTCTGTCCGT-1

using 54 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[54]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.925 = ATAACGCTCTTGGGTA-1

using 73 reads

====================================================================================

graph has 90 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[2, 3, 67]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.931 = ATAAGAGAGCAATCTC-1

using 51 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[51]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.932 = ATAAGAGAGCATCATC-1

using 1879 reads

====================================================================================

graph has 620 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 1[1877]
surviving nonsolo ucounts = 1[1877]
ids = [2]

====================================================================================

UMI info for barcode ATAAGAGAGCATCATC-1 contig 1 = CTCATTCCTG...
umi GAGCAGGTTT = 1858 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=469]
0-23 ==> 51-74 on |591|TRBV2|5'UTR| [len=74] (mis=0)
23-364 ==> 0-341 on |592|TRBV2|L-REGION+V-REGION| [len=347] (mis=0)
368-417 ==> 0-49 on |546|TRBJ2-3|J-REGION| [len=49] (mis=1)
417-469 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 1 umis using 126 reads
cdr3 = CASSLITDTQYF at 353, score = 6 + 8
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 1827
start codons at 23, 40, 122, 234, 264, 291, 344
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.948 = ATAAGAGAGTACGCGA-1

using 78 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[2, 6, 68]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.950 = ATAAGAGAGTATGACA-1

using 8939 reads

====================================================================================

graph has 6070 edges initially, 220 edges after simplification

total ucounts = 620
nonsolo ucounts = 443[2^59, 3^36, 4^23, 5^23, 6^20, 7^14, 8^19, 9^16, 10^15, 11^8, 12^13, 13^13, 14^10, 15^13, 16^17, 17^5, 18^8, 19^9, 20^8, 21^3, 22^4, 23^5, 24^6, 25^4, 26^2, 27^3, 28^4, 29^7, 30^7, 31, 32^3, 33^5, 34^2, 35, 36^2, 37^2, 39^3, 40^2, 41^2, 42^4, 43^6, 45, 46^4, 49, 50, 51^5, 52^2, 53, 55, 57, 58, 59, 62^2, 64, 65, 68, 70, 75, 79, 80, 82, 84^2, 85, 95, 114, 117, 1415]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.961 = ATAAGAGCAATCTACG-1

using 405 reads

====================================================================================

graph has 162 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[4^3, 391]
surviving nonsolo ucounts = 1[391]
ids = [3]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.964 = ATAAGAGCACAGGTTT-1

using 44 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[43]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.967 = ATAAGAGCACCGAAAG-1

using 8240 reads

====================================================================================

graph has 3807 edges initially, 82 edges after simplification

total ucounts = 390
nonsolo ucounts = 248[2^47, 3^24, 4^17, 5^12, 6^14, 7^11, 8^9, 9^11, 10^8, 11^9, 12^4, 13^10, 14^5, 15^5, 16^3, 17^7, 18^4, 19^4, 20^2, 21^2, 22, 24^3, 25^4, 26^3, 27, 28^3, 29^2, 30^2, 32, 33, 34^2, 35, 37, 40^3, 41, 42, 44, 50, 52, 66, 67^2, 82, 93, 130, 5013]
surviving nonsolo ucounts = 1[5013]
ids = [364]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.970 = ATAAGAGCACGCCAGT-1

using 44 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[44]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.972 = ATAAGAGCAGACAAGC-1

using 75 reads

====================================================================================

graph has 62 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 4[2, 5, 6, 59]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.974 = ATAAGAGCAGATTGCT-1

using 105 reads

====================================================================================

graph has 54 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 3[4, 5, 93]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.975 = ATAAGAGCAGCTCGAC-1

using 52 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[52]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.976 = ATAAGAGCAGGCTGAA-1

using 47885 reads

====================================================================================

graph has 17265 edges initially, 206 edges after simplification

total ucounts = 635
nonsolo ucounts = 436[2^47, 3^39, 4^30, 5^32, 6^24, 7^12, 8^15, 9^15, 10^21, 11^11, 12^11, 13^12, 14^7, 15^7, 16^3, 17^8, 18^5, 19^12, 20^12, 21^6, 22^12, 23^3, 24^6, 25^6, 26^7, 27^2, 28^3, 29^2, 30, 31^4, 32^3, 33^4, 34^4, 36, 37^2, 38, 39^3, 43, 45, 46, 51, 53, 54, 57, 58, 59, 61, 62, 68, 76, 77, 79, 80, 109, 115, 118, 422, 485, 535, 593, 1190, 1208, 1212, 1252, 1345, 1371, 1467, 1487, 1607, 1653, 1817, 1879, 2006, 2034, 2160, 2312, 2350, 2455, 3142, 5941]
surviving nonsolo ucounts = 24[422, 485, 535, 593, 1190, 1208, 1212, 1252, 1345, 1371, 1467, 1487, 1607, 1653, 1817, 1879, 2006, 2034, 2160, 2312, 2350, 2455, 3142, 5941]
ids = [403, 436, 335, 616, 145, 619, 566, 120, 613, 260, ...]

====================================================================================

UMI info for barcode ATAAGAGCAGGCTGAA-1 contig 1 = GGGGACTGTG...
umi AACATAGCTC = 2043 reads: +400 validated
umi CAATGTCCGC = 1256 reads: +400 validated
umi CATAATTGAT = 1168 reads: +400 validated
umi CGCTGGTCCT = 2362 reads: +400 validated
umi CGTTGGTTGT = 1504 reads: +400 validated
umi CTCCTTGCAA = 1378 reads: +400 validated
umi CTGCTGCTTA = 3135 reads: +400 validated
umi GAGTGCTAGC = 1888 reads: +400 validated
umi GCGTATTTGT = 2464 reads: +400 validated
umi GTTCTATTGC = 1658 reads: +400 validated
umi TACCGCTCCC = 480 reads: +400 validated
umi TATCGATTCG = 2006 reads: +400 validated
umi TCGTTCCTGG = 1829 reads: +400 validated
umi TTGGCACACC = 1346 reads: +400 validated
umi TTGGTACACC = 600 reads: +400 validated
umi TTGTCTTACT = 1208 reads: +400 validated

UMI info for barcode ATAAGAGCAGGCTGAA-1 contig 2 = GATCTTAATT...
umi GCGTTGTTGG = 491 reads: +394 validated
umi GCTCTACGCA = 2143 reads: +394 validated
umi GTGTTTCGGG = 413 reads: +394 validated
umi TGTTAACGAC = 1185 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=558]
106-453 ==> 0-347 on |688|TRBV7-9|L-REGION+V-REGION| [len=347] (mis=4)
459-506 ==> 0-47 on |550|TRBJ2-7|J-REGION| [len=47] (mis=5)
506-558 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 15 umis using 1566 reads
cdr3 = CASSSRGGWREQYF at 436, score = 7 + 8
umis assigned: [11, 120, 145, 227, 241, 260, 271, 309, 333, 409] and 6 others
of which 16 are surviving nonsolos
reads assigned: 25764
start codons at 106, 130, 215, 314, 427
confident = true

TIG 2[bases=459]
0-23 ==> 0-23 on |463|TRAV13-1|5'UTR| [len=23] (mis=0)
23-360 ==> 0-337 on |464|TRAV13-1|L-REGION+V-REGION| [len=337] (mis=4)
353-417 ==> 0-64 on |395|TRAJ10|J-REGION| [len=64] (mis=4)
417-459 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 4 umis using 255 reads
cdr3 = CAADTGGGNKLTF at 347, score = 9 + 8
umis assigned: [335, 342, 403, 566]
of which 4 are surviving nonsolos
reads assigned: 4149
start codons at 23, 78, 87, 237
confident = true

REJECT CONTIGS

TIG 1[bases=506]
0-47 ==> 8686-8733 on segment before TRBV7-9 exon 1 [len=8733] (mis=0)
39-60 ==> 1110-1131 on segment before TRBV6-2 exon 1 [len=3166] (mis=1)
47-389 ==> 0-342 on |733|TRBV11-2|L-REGION+V-REGION| [len=342] (mis=1)
407-454 ==> 0-47 on |550|TRBJ2-7|J-REGION| [len=47] (mis=3)
454-506 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
umis assigned: [107, 175, 376]
of which 3 are surviving nonsolos
reads assigned: 8904
start codons at 47, 189, 270
confident = false
did not find CDR3

TIG 2[bases=447]
25-360 ==> 0-335 on |703|TRDV1|L-REGION+V-REGION| [len=347] (mis=0)
364-430 ==> 0-66 on |440|TRAJ53|J-REGION| [len=66] (mis=4)
cdr3 = CGTRSGGSNYKLTF at 357, score = 3 + 9
umis assigned: [359]
of which 1 are surviving nonsolos
reads assigned: 1428
start codons at 18, 25, 121, 158, 217, 245, 254
confident = false
not full
not full
now this is a cell
paired!

ACCCAACCTGAAGACTCGGCTGTCTACTTCTGTGCAGCAGACACGGGAGGAGGAAACAAACTCACCTTTGGGACAGGCACTCAGCTAAAAGTGGAACTCA <==> ACAGAGCAGGGGGACTCGGCCATGTATCTCTGTGCCAGCAGCTCCCGGGGGGGCTGGAGGGAGCAGTACTTCGGGCCGGGCACCAGGCTCACGGTCACAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.977 = ATAAGAGCAGGGATTG-1

using 99 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[98]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.978 = ATAAGAGCAGTAAGAT-1

using 448 reads

====================================================================================

graph has 420 edges initially, 14 edges after simplification

total ucounts = 29
nonsolo ucounts = 24[2^3, 4^2, 5^3, 6, 8^2, 9, 11, 14^2, 15, 20, 25, 26, 34, 36, 38, 66, 84]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.980 = ATAAGAGCATAACCTG-1

using 39 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.982 = ATAAGAGCATAGACTC-1

using 52 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[52]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.985 = ATAAGAGCATGAGCGA-1

using 307 reads

====================================================================================

graph has 210 edges initially, 10 edges after simplification

total ucounts = 14
nonsolo ucounts = 13[2, 4^2, 7, 9, 10, 17, 20, 21, 23, 31, 58, 100]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.990 = ATAAGAGGTACAGTTC-1

using 74 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[74]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.996 = ATAAGAGGTATAAACG-1

using 281 reads

====================================================================================

graph has 296 edges initially, 8 edges after simplification

total ucounts = 15
nonsolo ucounts = 12[2^2, 3, 6, 8, 10, 18, 22, 27, 57, 60, 63]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.997 = ATAAGAGGTATATGAG-1

using 52 reads

====================================================================================

graph has 58 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[2, 24^2]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1002 = ATAAGAGGTCCGAATT-1

using 23 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1008 = ATAAGAGGTCGCATCG-1

using 76 reads

====================================================================================

graph has 82 edges initially, 4 edges after simplification

total ucounts = 8
nonsolo ucounts = 5[2, 4, 7, 19, 41]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1010 = ATAAGAGGTCTAGTCA-1

using 5510 reads

====================================================================================

graph has 4534 edges initially, 188 edges after simplification

total ucounts = 522
nonsolo ucounts = 379[2^59, 3^35, 4^21, 5^24, 6^19, 7^21, 8^17, 9^9, 10^9, 11^11, 12^10, 13^7, 14^7, 15^5, 16^9, 17^6, 18^6, 19^8, 20^5, 21^6, 22^5, 23^3, 24^2, 25^6, 26^2, 27^5, 28^9, 29^2, 30^4, 31^2, 33^7, 34^2, 35, 36^2, 37^3, 38^3, 39^4, 40^3, 41^3, 44^3, 45, 47, 49, 51, 52, 53, 54, 60, 64, 67, 72, 76, 108, 119]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1015 = ATAAGAGGTGATGTCT-1

using 141 reads

====================================================================================

graph has 162 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 8[4^2, 6, 7, 10, 13, 32, 64]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1019 = ATAAGAGGTGTGGTTT-1

using 103 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[103]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1026 = ATAAGAGTCAATCACG-1

using 60 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[60]
surviving nonsolo ucounts = 1[60]
ids = [0]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1033 = ATAAGAGTCATCTGTT-1

using 82 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[82]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1034 = ATAAGAGTCCACGAAT-1

using 63 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[31, 32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1037 = ATAAGAGTCCATGAGT-1

using 94 reads

====================================================================================

graph has 96 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 7[6, 7, 8, 10, 11^2, 37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1040 = ATAAGAGTCCGCATCT-1

using 99 reads

====================================================================================

graph has 54 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[99]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1048 = ATAAGAGTCGGCATCG-1

using 189 reads

====================================================================================

graph has 66 edges initially, 4 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[80, 109]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1052 = ATAAGAGTCTAACGGT-1

using 84 reads

====================================================================================

graph has 66 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 5[10, 11, 17, 19, 25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1054 = ATAAGAGTCTACTCAT-1

using 438 reads

====================================================================================

graph has 365 edges initially, 14 edges after simplification

total ucounts = 11
nonsolo ucounts = 11[2, 5, 7, 17, 25, 46, 50, 54, 59, 74, 99]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1055 = ATAAGAGTCTAGCACA-1

using 52 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 2[2, 46]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1057 = ATAAGAGTCTGACCTC-1

using 89 reads

====================================================================================

graph has 62 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 1[85]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1061 = ATAGACCAGAATTCCC-1

using 36 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1066 = ATAGACCAGAGCAATT-1

using 45 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 3[12^2, 18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1071 = ATAGACCAGATCCTGT-1

using 58 reads

====================================================================================

graph has 62 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 4[10, 13, 16, 18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1073 = ATAGACCAGATGAGAG-1

using 119 reads

====================================================================================

graph has 126 edges initially, 6 edges after simplification

total ucounts = 15
nonsolo ucounts = 8[3, 7^2, 12, 13, 15, 17, 38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1077 = ATAGACCAGCAGATCG-1

using 53 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[53]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1079 = ATAGACCAGCGCTTAT-1

using 33 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1081 = ATAGACCAGCTATGCT-1

using 49 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 8[2^2, 3^3, 6, 8, 22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1082 = ATAGACCAGCTCCCAG-1

using 101 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[101]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1083 = ATAGACCAGCTCCTTC-1

using 50 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[50]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1089 = ATAGACCAGGCGATAC-1

using 87 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[87]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1092 = ATAGACCAGGCTCATT-1

using 39 reads

====================================================================================

graph has 52 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 6[3^2, 4, 5, 9, 12]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1093 = ATAGACCAGGGTCGAT-1

using 42 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[2, 40]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1094 = ATAGACCAGGTAGCTG-1

using 48 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[23, 24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1095 = ATAGACCAGGTGACCA-1

using 54 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[3, 51]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1098 = ATAGACCAGTAGGTGC-1

using 21 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1102 = ATAGACCAGTCTCGGC-1

using 44 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 3[3, 11, 27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1113 = ATAGACCCAAGTTAAG-1

using 25 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1120 = ATAGACCCACACGCTG-1

using 27 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1123 = ATAGACCCACATCTTT-1

using 159 reads

====================================================================================

graph has 86 edges initially, 4 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[57, 102]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1127 = ATAGACCCACGCGAAA-1

using 65 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[65]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1128 = ATAGACCCACGGATAG-1

using 23445 reads

====================================================================================

graph has 13606 edges initially, 388 edges after simplification

total ucounts = 1556
nonsolo ucounts = 1095[2^165, 3^128, 4^78, 5^67, 6^58, 7^61, 8^54, 9^49, 10^38, 11^41, 12^26, 13^24, 14^19, 15^15, 16^30, 17^23, 18^21, 19^26, 20^19, 21^9, 22^14, 23^17, 24^10, 25^10, 26^10, 27^9, 28^5, 29^6, 30^2, 31^2, 32^3, 33^6, 34^4, 35^4, 37^2, 38^6, 39, 41, 42, 44^2, 45, 46, 47, 49, 51, 52, 53, 56, 58, 59, 63, 68, 79, 80, 83, 85, 89, 93, 97^3, 99, 138, 346, 551, 1090, 1220, 3664, 3952]
surviving nonsolo ucounts = 7[68, 346, 551, 1090, 1220, 3664, 3952]
ids = [91, 110, 1031, 981, 1095, 427, 1045]

====================================================================================

REJECT CONTIGS

TIG 1[bases=314]
4-212 ==> 3018-3226 on segment before TRBJ2-1 exon 1 [len=3226] (mis=0)
212-262 ==> 0-50 on |543|TRBJ2-1|J-REGION| [len=50] (mis=0)
262-314 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
umis assigned: [110, 427, 981]
of which 3 are surviving nonsolos
reads assigned: 4986
start codons at 71, 220
confident = false
did not find CDR3

TIG 2[bases=444]
4-41 ==> 49-86 on segment before TRBJ2-2P exon 1 [len=86] (mis=0)
41-87 ==> 0-46 on |545|TRBJ2-2P|J-REGION| [len=46] (mis=0)
87-191 ==> 0-104 on segment before TRBJ2-3 exon 1 [len=104] (mis=0)
240-342 ==> 0-102 on segment before TRBJ2-4 exon 1 [len=102] (mis=0)
392-444 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
umis assigned: [1031, 1045, 1430]
of which 2 are surviving nonsolos
reads assigned: 4399
start codons at 101
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1131 = ATAGACCCACTCAGGC-1

using 430 reads

====================================================================================

graph has 332 edges initially, 10 edges after simplification

total ucounts = 20
nonsolo ucounts = 16[2^2, 3^2, 4^2, 5, 6, 9, 13, 16, 25, 61, 74, 95, 104]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1136 = ATAGACCCAGCGTAAG-1

using 26 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1137 = ATAGACCCAGCTTCGG-1

using 269 reads

====================================================================================

graph has 263 edges initially, 8 edges after simplification

total ucounts = 24
nonsolo ucounts = 20[2^2, 3^3, 5^2, 9^2, 12, 13, 14, 17, 18, 21, 22, 23, 25, 28, 31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1138 = ATAGACCCAGGACCCT-1

using 50 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[50]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1139 = ATAGACCCAGGCTGAA-1

using 1232 reads

====================================================================================

graph has 370 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[1231]
surviving nonsolo ucounts = 1[1231]
ids = [1]

====================================================================================

UMI info for barcode ATAGACCCAGGCTGAA-1 contig 1 = TGGGGACTGT...
umi GTTTTTAGCA = 1231 reads: +400 validated

GOOD CONTIGS

TIG 1[bases=559]
107-454 ==> 0-347 on |688|TRBV7-9|L-REGION+V-REGION| [len=347] (mis=4)
460-507 ==> 0-47 on |550|TRBJ2-7|J-REGION| [len=47] (mis=5)
507-559 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 1 umis using 37 reads
cdr3 = CASSSRGGWREQYF at 437, score = 7 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 1211
start codons at 107, 131, 216, 315, 428
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1140 = ATAGACCCAGTATCTG-1

using 42 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[18, 24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1142 = ATAGACCCAGTTAACC-1

using 12 reads

====================================================================================

graph has 6 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[12]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1143 = ATAGACCCATAACCTG-1

using 148 reads

====================================================================================

graph has 52 edges initially, 4 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[30, 117]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1146 = ATAGACCCATATGGTC-1

using 32 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1148 = ATAGACCCATGACGGA-1

using 51 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[51]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1149 = ATAGACCCATGGTCAT-1

using 59 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 4[2, 4, 21, 32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1154 = ATAGACCGTAAAGGAG-1

using 80 reads

====================================================================================

graph has 64 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 5[2, 4^2, 5, 64]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1157 = ATAGACCGTAATAGCA-1

using 1916 reads

====================================================================================

graph has 702 edges initially, 4 edges after simplification

total ucounts = 10
nonsolo ucounts = 4[4, 28, 35, 1843]
surviving nonsolo ucounts = 1[1843]
ids = [2]

====================================================================================

UMI info for barcode ATAGACCGTAATAGCA-1 contig 1 = GGGACAGTGA...
umi ATGACGTCGG = 1841 reads: +403 validated

GOOD CONTIGS

TIG 1[bases=505]
0-50 ==> 0-50 on |678|TRBV7-3|5'UTR| [len=50] (mis=3)
50-397 ==> 0-347 on |679|TRBV7-3|L-REGION+V-REGION| [len=347] (mis=4)
404-453 ==> 0-49 on |546|TRBJ2-3|J-REGION| [len=49] (mis=4)
453-505 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 1 umis using 124 reads
cdr3 = CASSSLAHGVDTQYF at 380, score = 8 + 8
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 1810
start codons at 50, 159, 273
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1158 = ATAGACCGTACCGGCT-1

using 68 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[68]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1159 = ATAGACCGTACCGTTA-1

using 369 reads

====================================================================================

graph has 461 edges initially, 14 edges after simplification

total ucounts = 26
nonsolo ucounts = 16[3, 4, 6^2, 9, 11, 16, 19, 23, 28, 30^2, 41, 43, 44, 46]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1163 = ATAGACCGTAGCTAAA-1

using 40 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 3[3, 8, 26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1166 = ATAGACCGTATAGGTA-1

using 55 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 5[2^2, 3, 12, 35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1171 = ATAGACCGTCGAAAGC-1

using 70 reads

====================================================================================

graph has 62 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 5[4, 5, 6, 10, 44]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1173 = ATAGACCGTCTACCTC-1

using 47 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[47]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1174 = ATAGACCGTCTGGTCG-1

using 30 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1177 = ATAGACCGTGCATCTA-1

using 29 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[13, 16]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1180 = ATAGACCGTTAAGACA-1

using 84 reads

====================================================================================

graph has 78 edges initially, 2 edges after simplification

total ucounts = 14
nonsolo ucounts = 10[2, 3, 4^2, 5, 6^2, 10, 15, 25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1182 = ATAGACCGTTACGACT-1

using 50 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[18, 30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1183 = ATAGACCGTTAGAACA-1

using 39 reads

====================================================================================

graph has 80 edges initially, 4 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[39]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1184 = ATAGACCGTTAGTGGG-1

using 290 reads

====================================================================================

graph has 298 edges initially, 10 edges after simplification

total ucounts = 21
nonsolo ucounts = 15[2^5, 5^2, 13, 17, 20, 33, 34, 37, 44, 66]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1185 = ATAGACCGTTCATGGT-1

using 34 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1186 = ATAGACCGTTCCCTTG-1

using 64 reads

====================================================================================

graph has 68 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[64]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1187 = ATAGACCGTTCCTCCA-1

using 18 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1189 = ATAGACCGTTGTACAC-1

using 75 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[75]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1191 = ATAGACCGTTTGGCGC-1

using 15 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1192 = ATAGACCGTTTGTTGG-1

using 2484 reads

====================================================================================

graph has 2200 edges initially, 82 edges after simplification

total ucounts = 322
nonsolo ucounts = 220[2^39, 3^20, 4^22, 5^20, 6^11, 7^9, 8^8, 9^12, 10^10, 11^7, 12^8, 13^4, 14^3, 15^4, 16^4, 17^4, 18^4, 19^2, 20^2, 21^2, 22^2, 24^2, 25^2, 26, 27, 30, 31, 32, 35, 36^2, 38^2, 39, 40, 42, 44, 50, 68, 70, 82, 98]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1193 = ATAGACCTCAACACTG-1

using 40 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[18, 21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1195 = ATAGACCTCAATAAGG-1

using 22 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1197 = ATAGACCTCACCTCGT-1

using 25 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1201 = ATAGACCTCCAAGCCG-1

using 62 reads

====================================================================================

graph has 64 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 5[2, 9, 11, 16, 22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1203 = ATAGACCTCCACTCCA-1

using 46 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[22, 23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1204 = ATAGACCTCCAGATCA-1

using 86 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[86]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1208 = ATAGACCTCCGCAGTG-1

using 72 reads

====================================================================================

graph has 48 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[2, 69]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1209 = ATAGACCTCCGTCAAA-1

using 160 reads

====================================================================================

graph has 156 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 8[3^2, 6, 7, 10, 11, 56, 61]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1210 = ATAGACCTCCGTTGCT-1

using 58 reads

====================================================================================

graph has 52 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 6[2, 3, 4, 6, 12, 29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1212 = ATAGACCTCCTAGAAC-1

using 81 reads

====================================================================================

graph has 64 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 5[2^3, 9, 61]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1213 = ATAGACCTCCTAGTGA-1

using 44 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 3[3, 15, 23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1214 = ATAGACCTCCTCCTAG-1

using 92 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[92]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1215 = ATAGACCTCCTGCAGG-1

using 51 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[51]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1224 = ATAGACCTCGTCCAGG-1

using 54 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[54]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1225 = ATAGACCTCGTTTATC-1

using 21 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1230 = ATAGACCTCTGAGTGT-1

using 58 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[58]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1232 = ATAGACCTCTGTCTCG-1

using 56 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[56]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1233 = ATAGACCTCTTACCTA-1

using 31 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1243 = ATCACGAAGAAGGGTA-1

using 1057 reads

====================================================================================

graph has 596 edges initially, 16 edges after simplification

total ucounts = 44
nonsolo ucounts = 35[2^4, 3, 5^2, 6, 7^2, 9, 11^3, 12^3, 13, 14^2, 17, 25, 38, 40, 41, 46, 52, 54, 59, 74, 75^2, 81, 95, 116]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1246 = ATCACGAAGACTAGAT-1

using 71 reads

====================================================================================

graph has 39 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[71]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1247 = ATCACGAAGACTCGGA-1

using 67 reads

====================================================================================

graph has 6 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[67]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1252 = ATCACGAAGAGTTGGC-1

using 110 reads

====================================================================================

graph has 36 edges initially, 4 edges after simplification

total ucounts = 3
nonsolo ucounts = 3[3, 40, 67]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1255 = ATCACGAAGATGCCAG-1

using 26287 reads

====================================================================================

graph has 7950 edges initially, 164 edges after simplification

total ucounts = 497
nonsolo ucounts = 343[2^52, 3^34, 4^28, 5^21, 6^24, 7^13, 8^18, 9^14, 10^11, 11^6, 12^8, 13^4, 14^9, 15^3, 16^9, 17^8, 18^4, 19^2, 20^5, 21^2, 22^2, 23^5, 24^5, 25^7, 26, 27, 28^2, 29^3, 30^3, 31, 32^4, 34, 35, 38^3, 39, 41^2, 42^3, 44^2, 45^2, 47^2, 50, 51^2, 53^2, 60^2, 63, 67, 69, 70, 72, 87, 88, 5846, 7841, 7996]
surviving nonsolo ucounts = 3[5846, 7841, 7996]
ids = [267, 326, 116]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1258 = ATCACGAAGCATCATC-1

using 92 reads

====================================================================================

graph has 64 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 3[3, 8, 81]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1260 = ATCACGAAGCCCAACC-1

using 30 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1261 = ATCACGAAGCCTCGTG-1

using 139 reads

====================================================================================

graph has 102 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 5[10, 12, 14, 20, 82]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1265 = ATCACGAAGCTAAACA-1

using 327 reads

====================================================================================

graph has 314 edges initially, 10 edges after simplification

total ucounts = 21
nonsolo ucounts = 16[3^4, 4, 6^2, 8, 9, 13, 25, 28, 31, 41, 67, 72]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1266 = ATCACGAAGCTAGCCC-1

using 25 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1267 = ATCACGAAGCTGAACG-1

using 181 reads

====================================================================================

graph has 208 edges initially, 10 edges after simplification

total ucounts = 14
nonsolo ucounts = 11[2^3, 3, 4, 7^2, 9, 34, 45, 63]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1271 = ATCACGAAGGCGACAT-1

using 20686 reads

====================================================================================

graph has 5457 edges initially, 50 edges after simplification

total ucounts = 171
nonsolo ucounts = 117[2^19, 3^19, 4^14, 5^8, 6^4, 7^2, 8^5, 9^3, 11^2, 12^3, 13, 15, 16, 17^2, 18, 19^2, 20, 21, 25^2, 26^2, 28^2, 34, 35, 37, 40, 42, 45, 46, 50, 65, 79, 90, 92, 115, 294, 784, 1229, 1323, 1514, 1557, 2105, 2212, 8157]
surviving nonsolo ucounts = 7[784, 1229, 1514, 1557, 2105, 2212, 8157]
ids = [67, 104, 2, 0, 129, 162, 50]

====================================================================================

UMI info for barcode ATCACGAAGGCGACAT-1 contig 1 = TGGGGACAGT...
umi AAAACGCCGC = 1564 reads: +391 validated
umi AAACAACCAG = 1521 reads: +391 validated
umi CAAAACCGCA = 7247 reads: -354 +4 -2XX +2 -1XX +2 -1XX +5 -1XX +2 -1XX +5 -1XX +2 -1XX +5 -1XX +1 invalidated
umi CCGTCCACTT = 790 reads: +391 validated
umi GCGAGGCACA = 1233 reads: +391 validated
umi TAATTTCCAA = 2185 reads: +147 -1XX +243 invalidated
umi TTGCCACGGT = 2214 reads: +391 validated

GOOD CONTIGS

TIG 1[bases=495]
52-399 ==> 0-347 on |688|TRBV7-9|L-REGION+V-REGION| [len=347] (mis=3)
393-443 ==> 0-50 on |543|TRBJ2-1|J-REGION| [len=50] (mis=5)
443-495 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 6 umis using 600 reads
cdr3 = CASSSAGEQFF at 382, score = 7 + 8
umis assigned: [0, 2, 50, 67, 104, 129, 162]
of which 7 are surviving nonsolos
reads assigned: 16430
start codons at 52, 76, 161, 260, 373
confident = true
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1272 = ATCACGAAGGCGATAC-1

using 586 reads

====================================================================================

graph has 386 edges initially, 26 edges after simplification

total ucounts = 20
nonsolo ucounts = 18[2^3, 5^2, 9, 10, 12^2, 13, 37, 43, 48, 65, 69, 71, 83, 96]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1278 = ATCACGAAGGTAAACT-1

using 59 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[59]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1280 = ATCACGAAGGTGACCA-1

using 343 reads

====================================================================================

graph has 130 edges initially, 8 edges after simplification

total ucounts = 4
nonsolo ucounts = 4[56, 86, 93, 108]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1283 = ATCACGAAGTCTCAAC-1

using 25 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1285 = ATCACGAAGTGTTGAA-1

using 52 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[52]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1291 = ATCACGACAAACTGCT-1

using 91 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[91]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1295 = ATCACGACAAGTCTAC-1

using 275 reads

====================================================================================

graph has 188 edges initially, 16 edges after simplification

total ucounts = 18
nonsolo ucounts = 16[3, 4^3, 5, 8, 9, 12, 13^2, 26, 28^2, 29, 34, 53]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1299 = ATCACGACAATGTTGC-1

using 106 reads

====================================================================================

graph has 60 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 5[2^3, 3, 93]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1301 = ATCACGACACAGATTC-1

using 214 reads

====================================================================================

graph has 114 edges initially, 4 edges after simplification

total ucounts = 11
nonsolo ucounts = 9[3, 4^2, 5^2, 28, 47, 48, 68]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1302 = ATCACGACACAGTCGC-1

using 44 reads

====================================================================================

graph has 66 edges initially, 2 edges after simplification

total ucounts = 13
nonsolo ucounts = 9[2^2, 3^2, 4^2, 6, 7, 8]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1303 = ATCACGACACATCTTT-1

using 64 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[64]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1306 = ATCACGACAGAAGCAC-1

using 48 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[48]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1308 = ATCACGACAGATGGGT-1

using 75 reads

====================================================================================

graph has 112 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 8[2, 3^2, 6, 8, 9, 15, 28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1310 = ATCACGACAGCCACCA-1

using 45 reads

====================================================================================

graph has 40 edges initially, 6 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[17, 28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1311 = ATCACGACAGCGATCC-1

using 55 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[55]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1315 = ATCACGACAGGGTTAG-1

using 621 reads

====================================================================================

graph has 356 edges initially, 8 edges after simplification

total ucounts = 22
nonsolo ucounts = 13[3, 7, 9, 10, 15, 22, 38, 40, 78, 84^2, 85, 137]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1316 = ATCACGACAGTTAACC-1

using 86 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[86]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1318 = ATCACGACATACGCTA-1

using 108 reads

====================================================================================

graph has 150 edges initially, 2 edges after simplification

total ucounts = 31
nonsolo ucounts = 13[2^6, 4, 5, 7, 9^2, 15, 29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1319 = ATCACGACATAGAAAC-1

using 63 reads

====================================================================================

graph has 54 edges initially, 4 edges after simplification

total ucounts = 5
nonsolo ucounts = 5[3, 4, 10, 23^2]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1320 = ATCACGACATCACCCT-1

using 90 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[90]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1321 = ATCACGACATCAGTAC-1

using 20 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1327 = ATCACGACATGTCTCC-1

using 33 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1330 = ATCACGAGTCATACTG-1

using 62 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[62]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1333 = ATCACGAGTCCATCCT-1

using 37 reads

====================================================================================

graph has 48 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 5[3, 4, 6, 10, 13]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1334 = ATCACGAGTCCTCCAT-1

using 21 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1335 = ATCACGAGTCGAATCT-1

using 46 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[46]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1338 = ATCACGAGTCTAAACC-1

using 32 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1339 = ATCACGAGTCTAACGT-1

using 31 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1340 = ATCACGAGTCTAGCGC-1

using 350 reads

====================================================================================

graph has 338 edges initially, 20 edges after simplification

total ucounts = 26
nonsolo ucounts = 22[2^2, 3^2, 5, 6^2, 7, 8, 9, 11, 14, 15, 18, 20, 22^2, 24, 26, 38, 41, 44]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1347 = ATCACGAGTGCGAAAC-1

using 6496 reads

====================================================================================

graph has 5145 edges initially, 152 edges after simplification

total ucounts = 676
nonsolo ucounts = 490[2^88, 3^58, 4^38, 5^30, 6^37, 7^24, 8^23, 9^15, 10^16, 11^10, 12^13, 13^8, 14^8, 15^9, 16^10, 17^4, 18^5, 19^7, 20^6, 21^4, 22^3, 23^4, 24^3, 25^2, 26, 27^5, 28^3, 29^2, 30, 31^2, 32, 33^6, 34^2, 35, 38, 39^2, 42^2, 43^2, 46, 47^2, 48^2, 50, 51, 52^2, 54^2, 56^4, 58^2, 59, 60^2, 65, 66, 74, 77, 78^2, 79, 80, 81^2, 89^2, 101, 117]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1349 = ATCACGAGTGCTGTAT-1

using 46 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[46]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1352 = ATCACGAGTGTGACGA-1

using 77 reads

====================================================================================

graph has 48 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[76]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1353 = ATCACGAGTGTGCCTG-1

using 74 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[74]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1354 = ATCACGAGTTAAGATG-1

using 144 reads

====================================================================================

graph has 116 edges initially, 8 edges after simplification

total ucounts = 11
nonsolo ucounts = 7[11, 14, 18^2, 22, 26, 31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1358 = ATCACGAGTTGCTCCT-1

using 91 reads

====================================================================================

graph has 52 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[91]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1359 = ATCACGAGTTGGTGGA-1

using 25 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1360 = ATCACGAGTTTAGCTG-1

using 41 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[2, 8, 30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1361 = ATCACGAGTTTCCACC-1

using 36 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1368 = ATCACGATCAGTCCCT-1

using 124 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[124]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1375 = ATCACGATCCATGAAC-1

using 74 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[17, 57]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1377 = ATCACGATCCCAGGTG-1

using 555 reads

====================================================================================

graph has 358 edges initially, 22 edges after simplification

total ucounts = 30
nonsolo ucounts = 19[2^2, 3, 6, 7^2, 8, 13, 18^2, 19, 24, 27^2, 48, 50, 62, 69, 134]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1383 = ATCACGATCGAATGGG-1

using 16170 reads

====================================================================================

graph has 9388 edges initially, 332 edges after simplification

total ucounts = 939
nonsolo ucounts = 703[2^111, 3^66, 4^59, 5^39, 6^39, 7^33, 8^27, 9^15, 10^22, 11^20, 12^24, 13^20, 14^17, 15^15, 16^11, 17^13, 18^15, 19^9, 20^16, 21^9, 22^6, 23^8, 24^6, 25^2, 26^4, 27^3, 28^3, 29^9, 30^5, 31^6, 32^5, 33^5, 34^4, 35^3, 36, 37, 39^2, 40, 41^6, 42, 43, 44^2, 46^2, 48, 49^2, 50, 52, 53^2, 55, 56^2, 57^3, 58^2, 59, 60, 61, 63, 64^2, 68, 69^2, 71, 72, 77, 79^2, 84, 87, 91, 106, 109, 210, 242, 6133]
surviving nonsolo ucounts = 3[210, 242, 6133]
ids = [794, 781, 169]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1386 = ATCACGATCGCGTTTC-1

using 43 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[43]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1388 = ATCACGATCGGCTACG-1

using 3707 reads

====================================================================================

graph has 3736 edges initially, 84 edges after simplification

total ucounts = 447
nonsolo ucounts = 329[2^58, 3^38, 4^29, 5^26, 6^19, 7^11, 8^15, 9^12, 10^4, 11^6, 12^9, 13^10, 14^9, 15^10, 16^9, 17^5, 18^9, 19^6, 20^2, 22^6, 23^5, 24^3, 25^2, 26^2, 27^4, 30^2, 31^2, 34, 35, 37, 38^2, 39^2, 40, 49^2, 53, 54, 64, 66, 83, 160]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1391 = ATCACGATCGTCACGG-1

using 61 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[61]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1395 = ATCACGATCTCAACTT-1

using 32 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1397 = ATCACGATCTCCCTGA-1

using 3562 reads

====================================================================================

graph has 1954 edges initially, 30 edges after simplification

total ucounts = 180
nonsolo ucounts = 116[2^18, 3^21, 4^5, 5^8, 6^9, 7^6, 8^8, 9^4, 10^8, 11^4, 12^3, 13, 14^3, 15^3, 16, 17, 20, 21, 22, 24^2, 25, 27, 30, 35, 94, 97, 126, 2305]
surviving nonsolo ucounts = 1[2305]
ids = [140]

====================================================================================

UMI info for barcode ATCACGATCTCCCTGA-1 contig 1 = TGGGAGAGAT...
umi TCGGTCCCAT = 2318 reads: +403 validated

GOOD CONTIGS

TIG 1[bases=509]
64-404 ==> 0-340 on |514|TRAV5|L-REGION+V-REGION| [len=340] (mis=0)
405-467 ==> 0-62 on |423|TRAJ37|J-REGION| [len=62] (mis=2)
467-509 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 1 umis using 127 reads
cdr3 = CAESIRSSNTGKLIF at 391, score = 7 + 8
umis assigned: [140]
of which 1 are surviving nonsolos
reads assigned: 2280
start codons at 64, 118, 134, 283, 289
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1404 = ATCACGATCTTATCTG-1

using 57 reads

====================================================================================

graph has 48 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[57]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1405 = ATCACGATCTTCAACT-1

using 10 reads

====================================================================================

graph has 4 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[10]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1408 = ATCACGATCTTGTCAT-1

using 72 reads

====================================================================================

graph has 94 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 6[2, 3, 4, 6, 13, 42]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1409 = ATCATCTAGAAACCAT-1

using 119 reads

====================================================================================

graph has 76 edges initially, 8 edges after simplification

total ucounts = 13
nonsolo ucounts = 8[4, 5, 6, 11, 16, 21, 24, 27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1410 = ATCATCTAGAACTGTA-1

using 12300 reads

====================================================================================

graph has 8272 edges initially, 402 edges after simplification

total ucounts = 670
nonsolo ucounts = 534[2^51, 3^36, 4^39, 5^28, 6^23, 7^28, 8^16, 9^9, 10^12, 11^10, 12^10, 13^9, 14^8, 15^9, 16^5, 17^8, 18^6, 19^14, 20^9, 21^10, 22^9, 23^13, 24^12, 25^11, 26^11, 27^18, 28^9, 29^7, 30^5, 31^9, 32^4, 33^2, 34^4, 35^7, 36^6, 37^4, 38^2, 39^6, 40^4, 41^5, 42^3, 43^6, 44^3, 45^2, 46^3, 48^3, 49^3, 50^2, 51^3, 55, 59, 64, 67, 69, 70, 74, 76, 77, 80, 89^3, 95, 104, 125, 136, 2385]
surviving nonsolo ucounts = 2[49, 2385]
ids = [660, 92]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1411 = ATCATCTAGAATAGGG-1

using 30 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1413 = ATCATCTAGACATAAC-1

using 14 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[14]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1417 = ATCATCTAGACGCAAC-1

using 319 reads

====================================================================================

graph has 244 edges initially, 8 edges after simplification

total ucounts = 31
nonsolo ucounts = 24[2^4, 3^3, 4^2, 5^3, 6, 7^3, 9, 10^2, 12, 14, 48, 50, 92]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1418 = ATCATCTAGACTTTCG-1

using 15 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1419 = ATCATCTAGAGACTTA-1

using 51 reads

====================================================================================

graph has 66 edges initially, 4 edges after simplification

total ucounts = 16
nonsolo ucounts = 9[2^2, 3, 4^3, 5, 6, 14]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1421 = ATCATCTAGAGTAAGG-1

using 78 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[78]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1422 = ATCATCTAGAGTACAT-1

using 116 reads

====================================================================================

graph has 106 edges initially, 6 edges after simplification

total ucounts = 12
nonsolo ucounts = 10[4, 6, 7, 9, 11, 12, 13, 14, 17, 21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1423 = ATCATCTAGATAGTCA-1

using 195 reads

====================================================================================

graph has 66 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[195]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1428 = ATCATCTAGCAGACTG-1

using 16826 reads

====================================================================================

graph has 6656 edges initially, 182 edges after simplification

total ucounts = 406
nonsolo ucounts = 300[2^39, 3^28, 4^23, 5^15, 6^8, 7^19, 8^6, 9^15, 10^8, 11^14, 12^3, 13^7, 14^7, 15^7, 16^5, 17^2, 18^4, 19^3, 20^9, 21^6, 22^3, 23^3, 24^6, 25^5, 26^5, 27^3, 28^2, 29^5, 30^2, 32^4, 33^3, 34^2, 35, 36^3, 37, 38^3, 39, 40, 41, 42^2, 43^2, 44, 48, 49, 52, 53, 54, 55, 65, 78, 993, 1056, 1294, 1902, 7416]
surviving nonsolo ucounts = 5[993, 1056, 1294, 1902, 7416]
ids = [267, 239, 53, 146, 169]

====================================================================================

UMI info for barcode ATCATCTAGCAGACTG-1 contig 1 = AGAACACATC...
umi CGGATTGGGG = 7421 reads: +161 -1XX +3 -4XX +2 -2XX +1 -5XX +1 -4XX +1 -2XX +1 -1XX +1 -186X +1 -14XX +2 -4XX +1 -2XX invalidated
umi GCGTATTCCT = 1056 reads: +400 validated

GOOD CONTIGS

TIG 1[bases=610]
0-168 ==> 52-220 on |525|TRAV8-6|5'UTR| [len=220] (mis=1)
168-509 ==> 0-341 on |526|TRAV8-6|L-REGION+V-REGION| [len=341] (mis=6)
505-568 ==> 0-63 on |425|TRAJ39|J-REGION| [len=63] (mis=4)
568-610 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 1 umis using 22 reads
cdr3 = CAVTLNNAGNMLTF at 495, score = 7 + 9
umis assigned: [169, 239]
of which 2 are surviving nonsolos
reads assigned: 8365
start codons at 168, 331, 514, 525, 555
confident = true

REJECT CONTIGS

TIG 1[bases=594]
2-22 ==> 9504-9524 on segment before TRBV21-1 exon 1 [len=9524] (mis=0)
72-185 ==> 0-113 on segment before TRBV21-1 exon 2 [len=113] (mis=0)
494-542 ==> 0-48 on |548|TRBJ2-5|J-REGION| [len=48] (mis=1)
542-594 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
umis assigned: [146, 267]
of which 2 are surviving nonsolos
reads assigned: 2857
start codons at 22, 253, 284, 344, 377, 396
confident = false
did not find CDR3

TIG 2[bases=509]
0-69 ==> 0-69 on |478|TRAV20|5'UTR| [len=69] (mis=0)
69-406 ==> 0-337 on |479|TRAV20|L-REGION+V-REGION| [len=337] (mis=3)
410-467 ==> 0-57 on |434|TRAJ47|J-REGION| [len=57] (mis=0)
467-509 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
umis assigned: [53]
of which 1 are surviving nonsolos
reads assigned: 1286
start codons at 69, 78, 409, 416
confident = false
frameshifted full length transcript of length 509
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1430 = ATCATCTAGCCTCGTG-1

using 34 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 5[2, 3, 6, 8, 12]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1431 = ATCATCTAGCGAAGGG-1

using 47 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 5[4^2, 5, 14, 18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1433 = ATCATCTAGCGTAATA-1

using 197 reads

====================================================================================

graph has 194 edges initially, 4 edges after simplification

total ucounts = 14
nonsolo ucounts = 13[2, 3, 4^3, 6, 7, 11, 15, 19, 20, 42, 59]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1434 = ATCATCTAGCGTTTAC-1

using 26 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1438 = ATCATCTAGGCATGTG-1

using 69 reads

====================================================================================

graph has 60 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 7[3, 4, 5^3, 9, 37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1439 = ATCATCTAGGCCCTCA-1

using 29 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1440 = ATCATCTAGGCGATAC-1

using 38 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1441 = ATCATCTAGGGATACC-1

using 102 reads

====================================================================================

graph has 52 edges initially, 4 edges after simplification

total ucounts = 12
nonsolo ucounts = 8[3^2, 6^2, 7, 9, 15, 49]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1442 = ATCATCTAGGGCTTCC-1

using 50230 reads

====================================================================================

graph has 16201 edges initially, 299 edges after simplification

total ucounts = 986
nonsolo ucounts = 738[2^67, 3^70, 4^58, 5^54, 6^39, 7^38, 8^28, 9^36, 10^29, 11^28, 12^26, 13^25, 14^18, 15^15, 16^16, 17^15, 18^12, 19^12, 20^9, 21^10, 22^7, 23^3, 24^5, 25^2, 26^4, 28^4, 29^4, 30^7, 31^3, 32^5, 33^5, 35, 37, 38^3, 41, 42, 43^2, 45^3, 46^3, 49^2, 50^4, 51^2, 52^2, 53^2, 54, 57, 58^2, 60^6, 61, 62^2, 66, 67, 70^2, 72^2, 73, 74, 75, 78, 79, 81, 82, 85, 93, 95, 117, 184, 302, 404, 415, 573, 923, 946, 1049, 1092, 1170, 1206, 1213, 1312, 1313, 1358, 1410, 1596, 1600, 1680, 1708, 1752, 1951, 1971, 2067, 2138, 2851, 6135]
surviving nonsolo ucounts = 25[302, 404, 415, 923, 946, 1049, 1092, 1170, 1206, 1213, 1312, 1313, 1358, 1410, 1596, 1600, 1680, 1708, 1752, 1951, 1971, 2067, 2138, 2851, 6135]
ids = [554, 909, 676, 815, 287, 60, 166, 395, 819, 578, ...]

====================================================================================

UMI info for barcode ATCATCTAGGGCTTCC-1 contig 1 = TGGGGAGAGT...
umi CTAGTATCGC = 1721 reads: +394 validated
umi CTCTGCGCCG = 1417 reads: +394 validated
umi TCATCTTTTC = 2108 reads: +18 -1XX +375 invalidated
umi TGATAATCCC = 2129 reads: +394 validated
umi TTTAGGTCGA = 1767 reads: +371 -1XX +2 -1 +19 invalidated

UMI info for barcode ATCATCTAGGGCTTCC-1 contig 2 = GTCATCCCTC...
umi AAAAATTTCA = 2853 reads: +394 validated
umi AGCGTGTGGC = 1314 reads: +394 validated
umi ATAGCCATGG = 1944 reads: +394 validated
umi ATAGGAGTCG = 1702 reads: +394 validated
umi CCTACCGCTA = 1358 reads: +394 validated
umi CGCTGGGCCT = 1601 reads: +394 validated
umi CTACCCCCCG = 1176 reads: +394 validated
umi GGGTCCTGTG = 1221 reads: +387 -7XX invalidated
umi GTATGATCTG = 1598 reads: +394 validated
umi TACGTACCTT = 1978 reads: +394 validated
umi TAGATAATTC = 419 reads: +394 validated
umi TGAGTGTTGC = 1203 reads: +394 validated
umi TGTGTGTCTG = 1316 reads: +394 validated

UMI info for barcode ATCATCTAGGGCTTCC-1 contig 3 = GAGAAGCCTC...
umi AATCGTCGCA = 1049 reads: +406 validated
umi AGGATATTAA = 1098 reads: +406 validated
umi CAGCTATTAT = 944 reads: +406 validated
umi GGAATATCGA = 302 reads: +406 validated
umi TGACCTATCA = 928 reads: +406 validated
umi TTCATTGGCA = 410 reads: +406 validated

GOOD CONTIGS

TIG 1[bases=520]
84-420 ==> 0-336 on |481|TRAV21|L-REGION+V-REGION| [len=336] (mis=3)
423-478 ==> 8-63 on |425|TRAJ39|J-REGION| [len=63] (mis=0)
478-520 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 5 umis using 567 reads
cdr3 = CAVVGNAGNMLTF at 408, score = 9 + 9
umis assigned: [399, 418, 740, 822, 959]
of which 5 are surviving nonsolos
reads assigned: 8966
start codons at 84, 128, 328, 424, 435, 465
confident = true

TIG 2[bases=524]
0-78 ==> 0-78 on |595|TRBV20-1|5'UTR| [len=78] (mis=0)
78-407 ==> 0-329 on |596|TRBV20-1|L-REGION+V-REGION| [len=335] (mis=0)
424-472 ==> 0-48 on |548|TRBJ2-5|J-REGION| [len=48] (mis=3)
472-524 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 13 umis using 1067 reads
cdr3 = CSALRAGGQGETQYF at 399, score = 7 + 7
umis assigned: [2, 163, 194, 196, 334, 369, 395, 578, 600, 669] and 3 others
of which 13 are surviving nonsolos
reads assigned: 19360
start codons at 21, 78, 216, 252, 258, 271, 331
confident = true

TIG 3[bases=565]
0-117 ==> 0-117 on |474|TRAV19|5'UTR| [len=117] (mis=1)
117-461 ==> 0-344 on |475|TRAV19|L-REGION+V-REGION| [len=350] (mis=1)
460-523 ==> 0-63 on |435|TRAJ48|J-REGION| [len=63] (mis=2)
523-565 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 5 umis using 172 reads
cdr3 = CALRSNFGNEKLTF at 450, score = 7 + 10
umis assigned: [60, 166, 287, 554, 815, 909]
of which 6 are surviving nonsolos
reads assigned: 4657
start codons at 111, 117, 174, 229, 250, 340, 349, 475
confident = true

REJECT CONTIGS

TIG 1[bases=311]
7-202 ==> 5805-6000 on segment before TRBD1 exon 1 [len=6000] (mis=0)
208-259 ==> 0-51 on |544|TRBJ2-2|J-REGION| [len=51] (mis=3)
259-311 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
umis assigned: [266, 271, 495]
of which 1 are surviving nonsolos
reads assigned: 6147
start codons at 79, 89, 163
confident = false
did not find CDR3
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1446 = ATCATCTAGGTCATCT-1

using 29 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1447 = ATCATCTAGGTCGGAT-1

using 44 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[2, 42]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1448 = ATCATCTAGGTGCAAC-1

using 60 reads

====================================================================================

graph has 66 edges initially, 4 edges after simplification

total ucounts = 6
nonsolo ucounts = 5[3^2, 5, 16, 32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1449 = ATCATCTAGGTGCACA-1

using 37 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 4[3, 9, 10, 15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1450 = ATCATCTAGGTGTGGT-1

using 190 reads

====================================================================================

graph has 116 edges initially, 4 edges after simplification

total ucounts = 22
nonsolo ucounts = 17[2^4, 3, 5^2, 8^2, 10, 11, 12, 13, 16, 22, 23, 41]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1451 = ATCATCTAGTAGGTGC-1

using 55 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[55]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1459 = ATCATCTAGTGTACGG-1

using 30640 reads

====================================================================================

graph has 9868 edges initially, 212 edges after simplification

total ucounts = 695
nonsolo ucounts = 503[2^72, 3^39, 4^37, 5^15, 6^19, 7^19, 8^24, 9^23, 10^13, 11^18, 12^22, 13^13, 14^14, 15^14, 16^10, 17^8, 18^10, 19^6, 20^12, 21^11, 22^9, 23^12, 24^2, 25^3, 26^6, 27, 28^4, 29^2, 30^4, 31^4, 32^3, 33^4, 34^2, 35, 36, 37^3, 38, 39, 40, 41^2, 43^2, 44^2, 45^5, 48, 50^2, 51, 53, 58, 62, 63^2, 69, 73^2, 92, 111, 304, 1080, 1083, 1236, 1248, 1251, 1411, 1412, 1506, 1706, 1790, 1809, 1819, 1820, 4429]
surviving nonsolo ucounts = 15[304, 1080, 1083, 1236, 1248, 1251, 1411, 1412, 1506, 1706, 1790, 1809, 1819, 1820, 4429]
ids = [615, 627, 31, 163, 510, 191, 285, 585, 116, 324, ...]

====================================================================================

UMI info for barcode ATCATCTAGTGTACGG-1 contig 1 = GCCAGGTTCA...
umi AATACCTCCT = 1057 reads: +412 validated
umi ATTCAGCTAT = 1299 reads: +66 -1XX +345 invalidated
umi CACGCATGCA = 1262 reads: +412 validated
umi GATATACATA = 4 reads: -400X +1 -1XX +2 -3XX +2 -1XX +1 -1XX invalidated
umi TGAGCCCTAG = 1419 reads: +412 validated
umi TTACTTACGC = 1107 reads: +412 validated

UMI info for barcode ATCATCTAGTGTACGG-1 contig 2 = TATGGGGATG...
umi ATAAGCTGCC = 1503 reads: +400 validated
umi ATCTCGGGCT = 1728 reads: -380X +1 -3XX +3 -5XX +1 -5XX +2 invalidated
umi ATGCTGTTCG = 1813 reads: +400 validated
umi CACTATTAAC = 1800 reads: +400 validated
umi CGTACCGGGT = 1402 reads: +400 validated
umi CTGAACTACA = 1700 reads: +400 validated
umi TACTTATCAA = 1257 reads: +400 validated
umi TGTCTCCATA = 301 reads: +400 validated
umi TTATCCTTCA = 1815 reads: +400 validated
umi TTGCTCTACG = 4427 reads: -92X +1 -2XX +305 invalidated

GOOD CONTIGS

TIG 1[bases=529]
0-75 ==> 0-75 on |467|TRAV14/DV4|5'UTR| [len=75] (mis=3)
75-425 ==> 0-350 on |468|TRAV14/DV4|L-REGION+V-REGION| [len=350] (mis=5)
426-487 ==> 0-61 on |450|TRAJ9|J-REGION| [len=61] (mis=3)
487-529 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 5 umis using 183 reads
cdr3 = CAMRERPNTGGFKTIF at 408, score = 7 + 8
umis assigned: [31, 163, 191, 374, 585, 627]
of which 5 are surviving nonsolos
reads assigned: 5934
start codons at 75, 165, 208, 229, 267, 295, 307, 399, 414
confident = true

TIG 2[bases=511]
0-59 ==> 0-59 on |569|TRBV12-4|5'UTR| [len=59] (mis=4)
59-406 ==> 0-347 on |570|TRBV12-4|L-REGION+V-REGION| [len=347] (mis=3)
412-459 ==> 0-47 on |550|TRBJ2-7|J-REGION| [len=47] (mis=2)
459-511 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 9 umis using 1122 reads
cdr3 = CASSSGTSAYEQYF at 389, score = 8 + 8
umis assigned: [116, 142, 148, 194, 285, 324, 510, 615, 634, 667]
of which 10 are surviving nonsolos
reads assigned: 17510
start codons at 1, 7, 59, 117, 158, 181, 230, 233, 282, 293, 320, 327
confident = true
now this is a cell
paired!

GGGGACTCAGCAATGTATTTCTGTGCAATGAGAGAGCGCCCGAATACTGGAGGCTTCAAAACTATCTTTGGAGCAGGAACAAGACTATTTGTTAAAGCAA <==> TCAGAACCCAGGGACTCAGCTGTGTACTTCTGTGCCAGCAGTTCCGGGACTAGCGCGTACGAGCAGTACTTCGGGCCGGGCACCAGGCTCACGGTCACAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1462 = ATCATCTAGTTACCCA-1

using 28 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1464 = ATCATCTAGTTGCAGG-1

using 17080 reads

====================================================================================

graph has 12267 edges initially, 524 edges after simplification

total ucounts = 1366
nonsolo ucounts = 1064[2^132, 3^106, 4^72, 5^70, 6^46, 7^42, 8^43, 9^47, 10^30, 11^31, 12^26, 13^20, 14^30, 15^27, 16^24, 17^25, 18^22, 19^20, 20^17, 21^20, 22^13, 23^16, 24^12, 25^9, 26^12, 27^7, 28^12, 29^14, 30^14, 31^7, 32^10, 33, 34^6, 35^6, 36^7, 37^4, 38^5, 39^2, 40^2, 41^2, 42^4, 43^2, 44, 45, 46^2, 47, 49^3, 50, 51^3, 52^3, 54^3, 55^2, 57, 59^2, 60, 61, 62, 64, 66, 70, 71^2, 72^2, 75^3, 77, 78, 81^2, 90, 101^2, 111, 132, 140, 1873]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1468 = ATCATCTCAAACTGCT-1

using 121 reads

====================================================================================

graph has 118 edges initially, 2 edges after simplification

total ucounts = 14
nonsolo ucounts = 9[2^2, 3^2, 5, 6, 8, 22, 65]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1471 = ATCATCTCAACCGCCA-1

using 65 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[65]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1476 = ATCATCTCAATAAGCA-1

using 189 reads

====================================================================================

graph has 88 edges initially, 6 edges after simplification

total ucounts = 8
nonsolo ucounts = 5[3, 5, 26, 54, 98]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1478 = ATCATCTCAATAGCGG-1

using 28 reads

====================================================================================

graph has 6 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1479 = ATCATCTCAATCCAAC-1

using 41 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[3, 6, 13, 17]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1481 = ATCATCTCAATCTACG-1

using 28 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1482 = ATCATCTCAATGGACG-1

using 63 reads

====================================================================================

graph has 58 edges initially, 6 edges after simplification

total ucounts = 11
nonsolo ucounts = 7[3, 4, 5, 6, 9, 16^2]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1483 = ATCATCTCAATGGAGC-1

using 34 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1486 = ATCATCTCACACCGAC-1

using 50 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[2, 11, 35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1487 = ATCATCTCACAGGCCT-1

using 62 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[62]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!
sorting bam, mem = 0.12
running samtools sort -l 8G -o /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_T_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk16-ucc6a7a72e6/files/contig_bam_sorted.bam /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_T_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk16-ucc6a7a72e6/files/contig_bam.bam

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

106.539 seconds used processing barcodes, peak mem = 0.27
