[stdout]

starting vdj_asm_asm, mem = 0.01
available mem = 833.21 GB
/scratch/etanis/software/cellranger-6.1.2/lib/bin/cr_vdj martian assembly main /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_T_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk08-ucc6a7a72e6 /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_T_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk08-ucc6a7a72e6/files /scratch/etanis/ST-118/multi/dimitri-multi/journal/SC_MULTI_CS.SC_MULTI_CORE.MULTI_GEM_WELL_PROCESSOR.VDJ_T_GEM_WELL_PROCESSOR.SC_VDJ_CONTIG_ASSEMBLER.ASSEMBLE_VDJ.fork0.chnk08.ucc6a7a72e6
n50_n50_rpu = 2159

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.0 = ACGCAGCAGGCATGGT-1

using 56694 reads

====================================================================================

graph has 15580 edges initially, 108 edges after simplification

total ucounts = 615
nonsolo ucounts = 373[2^71, 3^50, 4^34, 5^21, 6^24, 7^25, 8^6, 9^14, 10^11, 11^11, 12^7, 13^3, 14^8, 15^5, 16^5, 17^3, 18^7, 19^3, 20^3, 21^3, 22^6, 23^2, 24^2, 25, 27, 28, 30, 31^2, 33^2, 35, 39, 40^2, 44, 50, 52, 55, 57, 77, 79, 86, 90, 627, 630, 805, 869, 972, 1036, 1081, 1150, 1268^2, 1310, 1440, 1538, 1546, 1622, 1722, 1824, 1847, 1926, 1976, 2065, 2129, 2194, 2288, 2517, 2943, 5557, 7090]
surviving nonsolo ucounts = 27[627, 630, 805, 869, 1036, 1081, 1150, 1268^2, 1310, 1440, 1538, 1546, 1622, 1722, 1824, 1847, 1926, 1976, 2065, 2129, 2194, 2288, 2517, 2943, 5557, 7090]
ids = [298, 315, 270, 122, 344, 305, 509, 534, 554, 149, ...]

====================================================================================

UMI info for barcode ACGCAGCAGGCATGGT-1 contig 1 = ACAGTGATCC...
umi AACGTTACTT = 2075 reads: +400 validated
umi AGGCCCTAAG = 2117 reads: +400 validated
umi ATCATTTTGC = 872 reads: +400 validated
umi ATTGGATACT = 1304 reads: +400 validated
umi CAATATGTGT = 1857 reads: +400 validated
umi CATCATGTAT = 2202 reads: +400 validated
umi CGTCCAATTC = 804 reads: +400 validated
umi CTCCTTTGCA = 1084 reads: +400 validated
umi CTCTATATCT = 1539 reads: +400 validated
umi CTGAGCCGAT = 632 reads: +400 validated
umi GAGCTATTGA = 1546 reads: +400 validated
umi GCCAGTTTTG = 1933 reads: +400 validated
umi GTATATGTAG = 4823 reads: -380X +1 -3XX +3 -5XX +1 -5XX +2 invalidated
umi TCACCATTCG = 1618 reads: +400 validated
umi TCTCATTGGG = 1156 reads: +400 validated
umi TGCCCATCGC = 1149 reads: -384X +3 -5XX +1 -5XX +2 invalidated
umi TGTCCACTAT = 1439 reads: +400 validated
umi TTTTGCCCTT = 1986 reads: +400 validated

UMI info for barcode ACGCAGCAGGCATGGT-1 contig 2 = AAAGCAGATT...
umi ATGTCTATAT = 2882 reads: -14X +2 -3XX +1 -2XX +2 -3XX +1 -5XX +1 -3XX +2 -3XX +1 -2XX +1 -3XX +348 invalidated
umi CTATTCGAGC = 629 reads: +397 validated
umi GACGATTTCC = 1042 reads: +397 validated
umi TGTCGCTGGT = 1271 reads: +397 validated

UMI info for barcode ACGCAGCAGGCATGGT-1 contig 3 = GACTGTGATT...
umi CGACTACCCG = 2524 reads: +388 validated
umi CGGTATAAGG = 1731 reads: +388 validated
umi GATCTGGCGG = 7101 reads: +388 validated
umi GCGCGATGGG = 1751 reads: -349 +1 -2X +10 -1XX +5 -1XX +1 -1XX +3 -1XX +5 -1XX +1 -3XX +3 invalidated
umi TGCGCCTCTT = 2315 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=499]
0-47 ==> 0-47 on |674|TRBV7-2|5'UTR| [len=47] (mis=0)
47-394 ==> 0-347 on |675|TRBV7-2|L-REGION+V-REGION| [len=347] (mis=5)
400-447 ==> 0-47 on |550|TRBJ2-7|J-REGION| [len=47] (mis=5)
447-499 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 16 umis using 1501 reads
cdr3 = CASSPRHGGYEQYF at 377, score = 8 + 8
umis assigned: [20, 98, 122, 149, 160, 193, 270, 305, 307, 315] and 8 others
of which 18 are surviving nonsolos
reads assigned: 29709
start codons at 47, 156, 405
confident = true

TIG 2[bases=544]
0-105 ==> 0-105 on |459|TRAV12-2|5'UTR| [len=105] (mis=0)
105-438 ==> 0-333 on |460|TRAV12-2|L-REGION+V-REGION| [len=340] (mis=0)
443-502 ==> 7-66 on |432|TRAJ45|J-REGION| [len=66] (mis=0)
502-544 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 4 umis using 377 reads
cdr3 = CAWGGGGADGLTF at 432, score = 9 + 8
umis assigned: [136, 298, 344, 554]
of which 4 are surviving nonsolos
reads assigned: 5718
start codons at 16, 102, 105, 309, 325, 340, 379
confident = true

TIG 3[bases=486]
0-56 ==> 0-56 on |529|TRAV9-2|5'UTR| [len=56] (mis=1)
56-394 ==> 0-338 on |530|TRAV9-2|L-REGION+V-REGION| [len=338] (mis=3)
391-444 ==> 7-60 on |414|TRAJ29|J-REGION| [len=60] (mis=0)
444-486 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 4 umis using 882 reads
cdr3 = CALRGNTPLVF at 380, score = 9 + 9
umis assigned: [247, 265, 363, 392, 536]
of which 5 are surviving nonsolos
reads assigned: 15161
start codons at 15, 56, 131, 219, 273
confident = true
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.2 = ACGCAGCAGGCGATAC-1

using 46 reads

====================================================================================

graph has 52 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 5[2^2, 4, 6, 29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.3 = ACGCAGCAGGCTCATT-1

using 29 reads

====================================================================================

graph has 6 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.14 = ACGCAGCAGTGAAGAG-1

using 346 reads

====================================================================================

graph has 234 edges initially, 8 edges after simplification

total ucounts = 11
nonsolo ucounts = 10[2^4, 6, 10, 64, 67, 87, 103]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.19 = ACGCAGCAGTTGTCGT-1

using 38 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.20 = ACGCAGCAGTTTGCGT-1

using 73 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[73]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.34 = ACGCAGCCACATTTCT-1

using 46 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[46]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.41 = ACGCAGCCAGACGCAA-1

using 328 reads

====================================================================================

graph has 300 edges initially, 8 edges after simplification

total ucounts = 27
nonsolo ucounts = 20[2^4, 3, 5, 6^3, 7, 8, 9, 14^2, 18, 22, 26, 28, 69, 72]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.44 = ACGCAGCCAGCTGCTG-1

using 50 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[50]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.46 = ACGCAGCCAGGATTGG-1

using 129 reads

====================================================================================

graph has 276 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[129]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.48 = ACGCAGCCAGGTCGTC-1

using 276 reads

====================================================================================

graph has 199 edges initially, 6 edges after simplification

total ucounts = 16
nonsolo ucounts = 12[2^3, 3, 9, 19, 21, 23, 25, 44, 47, 75]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.52 = ACGCAGCCATCACGAT-1

using 74 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[74]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.56 = ACGCAGCCATGCCCGA-1

using 4937 reads

====================================================================================

graph has 3080 edges initially, 44 edges after simplification

total ucounts = 457
nonsolo ucounts = 245[2^63, 3^38, 4^24, 5^12, 6^15, 7^10, 8^14, 9^9, 10^8, 11^5, 12^4, 13^4, 14, 15^3, 16^4, 17^4, 18, 19^2, 20, 21, 23^3, 26, 27, 28^2, 29, 30, 31, 34^4, 46^2, 51, 60, 66, 144, 717, 1919]
surviving nonsolo ucounts = 4[66, 144, 717, 1919]
ids = [232, 289, 404, 396]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.58 = ACGCAGCCATGGGAAC-1

using 258 reads

====================================================================================

graph has 190 edges initially, 6 edges after simplification

total ucounts = 16
nonsolo ucounts = 10[3^3, 9^2, 10^2, 32, 76, 97]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.59 = ACGCAGCCATGGTCAT-1

using 163 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[163]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.64 = ACGCAGCCATTCCTCG-1

using 33 reads

====================================================================================

graph has 4 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.71 = ACGCAGCGTCAATACC-1

using 36 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.72 = ACGCAGCGTCAGAATA-1

using 4298 reads

====================================================================================

graph has 2256 edges initially, 54 edges after simplification

total ucounts = 272
nonsolo ucounts = 190[2^35, 3^32, 4^17, 5^17, 6^16, 7^8, 8^8, 9^5, 10^3, 11^8, 12^2, 13^2, 14^2, 17, 21, 23^2, 24, 26, 27^2, 28^2, 29, 32^2, 33, 34, 35^3, 38, 39, 42, 43, 45^2, 46, 47, 49, 52, 57^3, 72, 533, 779, 930]
surviving nonsolo ucounts = 4[72, 533, 779, 930]
ids = [199, 88, 213, 57]

====================================================================================

UMI info for barcode ACGCAGCGTCAGAATA-1 contig 1 = ACTATTTCTA...
umi ACGCGGCTGG = 1 reads: -371 +1 -2X +1 -2X +1 -1X +1 -1X +1 -3X invalidated
umi ATGATATCCA = 922 reads: +385 validated
umi GTTTAGCGGG = 55 reads: +274 -111 non-validated

GOOD CONTIGS

TIG 1[bases=503]
0-76 ==> 0-76 on |487|TRAV25|5'UTR| [len=76] (mis=0)
76-403 ==> 0-327 on |488|TRAV25|L-REGION+V-REGION| [len=327] (mis=1)
402-461 ==> 0-59 on |412|TRAJ27|J-REGION| [len=59] (mis=1)
461-503 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 1 umis using 38 reads
cdr3 = CAGNTNAGKSTF at 394, score = 6 + 8
umis assigned: [25, 57, 199]
of which 2 are surviving nonsolos
reads assigned: 960
start codons at 58, 76, 94, 105, 109, 128, 142, 164, 377, 410, 434
confident = true

REJECT CONTIGS

TIG 1[bases=495]
0-105 ==> 0-105 on |459|TRAV12-2|5'UTR| [len=105] (mis=0)
105-445 ==> 0-340 on |460|TRAV12-2|L-REGION+V-REGION| [len=340] (mis=1)
446-495 ==> 13-62 on |418|TRAJ32|J-REGION| [len=66] (mis=0)
cdr3 = CAVNHATNKLIF at 432, score = 9 + 8
umis assigned: [213]
of which 1 are surviving nonsolos
reads assigned: 679
start codons at 16, 102, 105, 309, 325, 340, 379, 445
confident = false
VJ delta = 28
not full
not full
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.76 = ACGCAGCGTCGGGTCT-1

using 114 reads

====================================================================================

graph has 80 edges initially, 2 edges after simplification

total ucounts = 24
nonsolo ucounts = 13[2^2, 3, 4^2, 5, 8^2, 11, 13, 14^2, 15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.78 = ACGCAGCGTCTCATCC-1

using 54 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[54]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.79 = ACGCAGCGTCTCCATC-1

using 10406 reads

====================================================================================

graph has 4316 edges initially, 54 edges after simplification

total ucounts = 298
nonsolo ucounts = 195[2^40, 3^26, 4^29, 5^23, 6^12, 7^11, 8^4, 9^7, 10^6, 11^4, 12^4, 13^3, 14, 15, 17, 20, 25, 26, 29, 37, 38, 40, 43, 46, 47, 54, 55, 65, 72, 109, 117, 399, 738, 1215, 1281, 1306, 1632, 2039]
surviving nonsolo ucounts = 9[55, 109, 117, 738, 1215, 1281, 1306, 1632, 2039]
ids = [203, 104, 4, 126, 165, 221, 111, 41, 256]

====================================================================================

UMI info for barcode ACGCAGCGTCTCCATC-1 contig 1 = GGAACTGTGT...
umi AGATTTTCCG = 1647 reads: +394 validated
umi CCCACTTTTG = 92 reads: -370 +1 -1XX +5 -2XX +4 -1XX +2 -1XX +1 -2XX +2 -2XX invalidated
umi GCCTCAGACA = 1211 reads: +394 validated
umi TCAATCAACA = 1286 reads: +394 validated

UMI info for barcode ACGCAGCGTCTCCATC-1 contig 2 = ATTCTTTCTT...
umi AACACATTCC = 117 reads: -14 +380 non-validated
umi CCTCTATTTG = 1305 reads: +394 validated
umi CTAATGCGAT = 744 reads: +394 validated
umi GCTTTTGGTC = 355 reads: -383X +1 -5XX +2 -1XX +2 invalidated
umi TACATAGCAT = 53 reads: +382 -7 +5 non-validated
umi TGCGTCTTAG = 2048 reads: -54X +1 -1XX +338 invalidated

GOOD CONTIGS

TIG 1[bases=464]
28-365 ==> 0-337 on |500|TRAV34|L-REGION+V-REGION| [len=337] (mis=1)
366-422 ==> 7-63 on |401|TRAJ17|J-REGION| [len=63] (mis=0)
422-464 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 3 umis using 254 reads
cdr3 = CGADAAAGNKLTF at 352, score = 7 + 9
umis assigned: [41, 104, 165, 221]
of which 4 are surviving nonsolos
reads assigned: 4161
start codons at 28, 176, 203, 226, 229, 260, 284, 335
confident = true

TIG 2[bases=466]
0-20 ==> 0-20 on |612|TRBV28|5'UTR| [len=20] (mis=0)
20-364 ==> 0-344 on |613|TRBV28|L-REGION+V-REGION| [len=344] (mis=3)
366-414 ==> 0-48 on |537|TRBJ1-2|J-REGION| [len=48] (mis=2)
414-466 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 4 umis using 336 reads
cdr3 = CASTVSRNYGYTF at 347, score = 6 + 9
umis assigned: [4, 111, 126, 174, 203, 256]
of which 5 are surviving nonsolos
reads assigned: 4561
start codons at 20, 78, 142, 155, 162, 170, 225, 228, 236, 338, 372
confident = true
now this is a cell
paired!

TCCCAGCCCAGCCATGCAGGCATCTACCTCTGTGGAGCAGACGCAGCTGCAGGCAACAAGCTAACTTTTGGAGGAGGAACCAGGGTGCTAGTTAAACCAA <==> TCCGCCAGCACCAACCAGACATCTATGTACCTCTGTGCCAGCACTGTATCCAGAAACTATGGCTACACCTTCGGTTCGGGGACCAGGTTAACCGTTGTAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.82 = ACGCAGCGTCTGCAAT-1

using 66 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[66]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.86 = ACGCAGCGTGCCTGTG-1

using 35 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 1[33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.88 = ACGCAGCGTGCTAGCC-1

using 58 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[58]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.95 = ACGCAGCGTTTGGCGC-1

using 38 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.96 = ACGCAGCTCAACCATG-1

using 50 reads

====================================================================================

graph has 44 edges initially, 4 edges after simplification

total ucounts = 8
nonsolo ucounts = 6[2^3, 3, 17, 22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.102 = ACGCAGCTCAGTACGT-1

using 62 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[62]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.107 = ACGCAGCTCCACGAAT-1

using 35 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 2[3, 26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.115 = ACGCAGCTCCTCGCAT-1

using 44 reads

====================================================================================

graph has 58 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[10, 34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.116 = ACGCAGCTCCTGTAGA-1

using 24 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.120 = ACGCAGCTCGCGTTTC-1

using 21 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.123 = ACGCAGCTCGGTCTAA-1

using 54 reads

====================================================================================

graph has 56 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[2, 22, 29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.128 = ACGCAGCTCTCTTGAT-1

using 120 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[120]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.131 = ACGCAGCTCTGTCAAG-1

using 1130 reads

====================================================================================

graph has 1209 edges initially, 26 edges after simplification

total ucounts = 225
nonsolo ucounts = 122[2^32, 3^17, 4^12, 5^10, 6^7, 7^5, 8^6, 9^3, 10^3, 11^3, 12, 13, 14^2, 15, 16^3, 17, 18, 19^3, 21^2, 22, 23, 25, 28, 30, 35, 42, 55, 89]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.133 = ACGCAGCTCTTCCTTC-1

using 31 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.135 = ACGCCAGAGAAGGGTA-1

using 35 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 1[33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.136 = ACGCCAGAGAATTGTG-1

using 22 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.138 = ACGCCAGAGACCTTTG-1

using 24 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.139 = ACGCCAGAGACGCACA-1

using 59 reads

====================================================================================

graph has 72 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 7[2, 3, 6, 7, 8, 13, 16]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.140 = ACGCCAGAGAGAACAG-1

using 35 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 12
nonsolo ucounts = 8[2^2, 3^2, 4, 5^2, 7]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.143 = ACGCCAGAGAGTCTGG-1

using 61 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[61]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.144 = ACGCCAGAGATACACA-1

using 78 reads

====================================================================================

graph has 128 edges initially, 2 edges after simplification

total ucounts = 14
nonsolo ucounts = 11[2^3, 3, 4^2, 6, 7, 8, 9, 28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.145 = ACGCCAGAGATAGTCA-1

using 69 reads

====================================================================================

graph has 82 edges initially, 2 edges after simplification

total ucounts = 14
nonsolo ucounts = 11[2^2, 3^2, 4^2, 6, 7, 9, 10, 16]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.146 = ACGCCAGAGATCGATA-1

using 31619 reads

====================================================================================

graph has 24393 edges initially, 805 edges after simplification

total ucounts = 3671
nonsolo ucounts = 2697[2^463, 3^325, 4^282, 5^212, 6^150, 7^150, 8^123, 9^115, 10^76, 11^95, 12^81, 13^64, 14^64, 15^34, 16^44, 17^51, 18^29, 19^29, 20^30, 21^16, 22^19, 23^14, 24^25, 25^9, 26^10, 27^8, 28^13, 29^11, 30^8, 31^11, 32^3, 33^6, 34^6, 35^7, 36^4, 37^5, 38^4, 39^5, 40^5, 41^5, 42^7, 43, 44^2, 45^3, 46, 47^3, 48^4, 49^3, 50^2, 51^2, 52^2, 53^2, 54, 55^4, 56^3, 57^2, 58^2, 59^3, 61, 62^2, 63, 65, 66^2, 67, 68, 69^2, 71^2, 72, 74^2, 75^2, 76, 78, 81, 82, 84, 85^2, 87, 89, 91^3, 92^2, 100, 102, 108, 118, 122, 127, 3405]
surviving nonsolo ucounts = 16[2^8, 3^5, 4, 6, 3405]
ids = [51, 424, 1083, 1641, 2385, 2386, 2471, 2472, 807, 871, ...]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.152 = ACGCCAGAGCCACGCT-1

using 197 reads

====================================================================================

graph has 126 edges initially, 4 edges after simplification

total ucounts = 15
nonsolo ucounts = 11[2^4, 3, 4, 5, 7, 12, 14, 140]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.158 = ACGCCAGAGCCTTGAT-1

using 10696 reads

====================================================================================

graph has 8471 edges initially, 192 edges after simplification

total ucounts = 1637
nonsolo ucounts = 1220[2^206, 3^171, 4^123, 5^114, 6^89, 7^73, 8^63, 9^53, 10^34, 11^49, 12^21, 13^26, 14^24, 15^16, 16^12, 17^15, 18^16, 19^17, 20^8, 21^11, 22^10, 23^4, 24^6, 25^7, 26^4, 27^3, 28^6, 29^5, 30^3, 31^2, 32, 33, 34, 35^2, 36^2, 37^3, 38^3, 39, 40, 41, 43, 46, 49, 50, 52^2, 57, 62, 84, 93, 96, 109, 111]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.163 = ACGCCAGAGCTGCAAG-1

using 49 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[49]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.168 = ACGCCAGAGGCGTACA-1

using 79 reads

====================================================================================

graph has 60 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[9, 68]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.171 = ACGCCAGAGGGATCTG-1

using 68 reads

====================================================================================

graph has 70 edges initially, 2 edges after simplification

total ucounts = 16
nonsolo ucounts = 9[2^3, 3^4, 9, 34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.174 = ACGCCAGAGGTCGGAT-1

using 12027 reads

====================================================================================

graph has 4488 edges initially, 46 edges after simplification

total ucounts = 301
nonsolo ucounts = 203[2^47, 3^29, 4^9, 5^20, 6^9, 7^7, 8^14, 9^3, 10^6, 11^5, 12^6, 13^4, 14^7, 15^2, 16^3, 17^3, 18^3, 19^2, 20^2, 22^2, 23^2, 24^2, 25, 26, 28, 29, 32, 36, 38, 43, 58, 340, 361, 374, 1051, 1061, 2154, 4978]
surviving nonsolo ucounts = 5[340, 1051, 1061, 2154, 4978]
ids = [111, 294, 261, 108, 207]

====================================================================================

UMI info for barcode ACGCCAGAGGTCGGAT-1 contig 1 = AGAACACATC...
umi TGAGAACCCG = 1086 reads: +143 -1XX +253 invalidated
umi TTTACATTAG = 1053 reads: +397 validated

UMI info for barcode ACGCCAGAGGTCGGAT-1 contig 2 = GGGGGATCTG...
umi AATCGCCTTC = 334 reads: -390X +1 -2XX +1 -4XX +2 invalidated
umi CCACGTCCTA = 2179 reads: +400 validated
umi CCATCTAGTA = 328 reads: +400 validated
umi GTGCTTGGTC = 5006 reads: -179X +221 invalidated

GOOD CONTIGS

TIG 1[bases=607]
0-168 ==> 52-220 on |525|TRAV8-6|5'UTR| [len=220] (mis=1)
168-509 ==> 0-341 on |526|TRAV8-6|L-REGION+V-REGION| [len=341] (mis=2)
504-565 ==> 0-61 on |450|TRAJ9|J-REGION| [len=61] (mis=4)
565-607 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 2 umis using 49 reads
cdr3 = CAVSDSGGFKTIF at 495, score = 7 + 8
umis assigned: [261, 294]
of which 2 are surviving nonsolos
reads assigned: 2087
start codons at 168, 331
confident = true

TIG 2[bases=492]
40-387 ==> 0-347 on |687|TRBV7-8|L-REGION+V-REGION| [len=347] (mis=1)
383-395 ==> 0-12 on |535|TRBD1|D-REGION| [len=12] (mis=3)
392-440 ==> 0-48 on |536|TRBJ1-1|J-REGION| [len=48] (mis=1)
440-492 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 3 umis using 690 reads
cdr3 = CASSLDRGNTEAFF at 370, score = 8 + 8
umis assigned: [17, 108, 111, 207]
of which 3 are surviving nonsolos
reads assigned: 7691
start codons at 40, 149, 182, 248
confident = true
now this is a cell
paired!

GTCCATATAAGCGACACGGCTGAGTACTTCTGTGCTGTGAGTGATAGTGGAGGCTTCAAAACTATCTTTGGAGCAGGAACAAGACTATTTGTTAAAGCAA <==> ACACAGCAGGAGGACTCCGCCGTGTATCTCTGTGCCAGCAGCTTAGACAGGGGGAACACTGAAGCTTTCTTTGGACAAGGCACCAGACTCACAGTTGTAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.178 = ACGCCAGAGTACGACG-1

using 25 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[2, 4, 17]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.181 = ACGCCAGAGTCAAGCG-1

using 21 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.182 = ACGCCAGAGTCAATAG-1

using 52 reads

====================================================================================

graph has 72 edges initially, 2 edges after simplification

total ucounts = 12
nonsolo ucounts = 8[2^2, 3, 4, 7^2, 11, 12]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.186 = ACGCCAGAGTCTCGGC-1

using 137 reads

====================================================================================

graph has 124 edges initially, 2 edges after simplification

total ucounts = 18
nonsolo ucounts = 10[2^3, 3, 4^3, 8, 11, 89]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.189 = ACGCCAGAGTGACTCT-1

using 2234 reads

====================================================================================

graph has 677 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 6[2^2, 3, 5^2, 2215]
surviving nonsolo ucounts = 1[2215]
ids = [7]

====================================================================================

UMI info for barcode ACGCCAGAGTGACTCT-1 contig 1 = GAGCTCATCT...
umi TCCGGTAGTC = 2228 reads: +397 validated

GOOD CONTIGS

TIG 1[bases=506]
0-67 ==> 80-147 on |470|TRAV17|5'UTR| [len=147] (mis=0)
67-404 ==> 0-337 on |471|TRAV17|L-REGION+V-REGION| [len=337] (mis=1)
400-464 ==> 0-64 on |395|TRAJ10|J-REGION| [len=64] (mis=5)
464-506 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 1 umis using 133 reads
cdr3 = CATDPTGGGNKLTF at 391, score = 6 + 8
umis assigned: [7]
of which 1 are surviving nonsolos
reads assigned: 2194
start codons at 67, 102, 179, 187, 281
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.193 = ACGCCAGAGTGGGTTG-1

using 68 reads

====================================================================================

graph has 70 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[68]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.198 = ACGCCAGCAAACGCGA-1

using 60 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[60]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.201 = ACGCCAGCAAAGGTGC-1

using 85 reads

====================================================================================

graph has 74 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[85]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.204 = ACGCCAGCAAGCTGAG-1

using 1508 reads

====================================================================================

graph has 1629 edges initially, 30 edges after simplification

total ucounts = 275
nonsolo ucounts = 171[2^37, 3^28, 4^16, 5^16, 6^6, 7^8, 8^4, 9^8, 10^7, 11^10, 12^5, 13, 14^2, 15^2, 16^2, 17^3, 18^2, 20, 21, 22, 23^2, 24^2, 25, 26, 33, 34, 41, 58, 101]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.206 = ACGCCAGCAATCGGTT-1

using 83 reads

====================================================================================

graph has 56 edges initially, 6 edges after simplification

total ucounts = 8
nonsolo ucounts = 6[2^3, 7, 34^2]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.207 = ACGCCAGCAATCTACG-1

using 163 reads

====================================================================================

graph has 202 edges initially, 4 edges after simplification

total ucounts = 15
nonsolo ucounts = 11[3, 4^2, 5, 13, 15^2, 21, 22, 26, 31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.208 = ACGCCAGCAATGGAGC-1

using 36 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.213 = ACGCCAGCACCAGTTA-1

using 15 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.215 = ACGCCAGCACGAAGCA-1

using 394 reads

====================================================================================

graph has 202 edges initially, 2 edges after simplification

total ucounts = 12
nonsolo ucounts = 6[2, 3, 10, 11, 16, 346]
surviving nonsolo ucounts = 1[346]
ids = [2]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.216 = ACGCCAGCACGAGAGT-1

using 16794 reads

====================================================================================

graph has 7655 edges initially, 156 edges after simplification

total ucounts = 1054
nonsolo ucounts = 738[2^133, 3^100, 4^82, 5^68, 6^52, 7^43, 8^43, 9^30, 10^17, 11^22, 12^17, 13^13, 14^19, 15^14, 16^10, 17^8, 18^9, 19^4, 20^2, 21^5, 22^2, 23^5, 24^2, 25^2, 27^3, 28^3, 29, 30, 31^6, 33, 34, 36, 40^2, 41, 44, 45^2, 51, 53, 59, 85, 201, 293, 375, 772, 1178, 1765, 1900, 1953, 2367]
surviving nonsolo ucounts = 9[201, 293, 375, 772, 1178, 1765, 1900, 1953, 2367]
ids = [585, 381, 835, 281, 121, 190, 185, 607, 567]

====================================================================================

UMI info for barcode ACGCCAGCACGAGAGT-1 contig 1 = ATTCTTTCTT...
umi AGGGGAAATG = 1784 reads: +415 validated
umi ATTGGTCCGC = 535 reads: +415 validated
umi CCACGATCTG = 249 reads: -388 +2 -1X +1 -1X +4 -1XX +6 -1XX +2 -1XX +2 -4XX +1 invalidated
umi CTCCACTCCT = 2380 reads: +415 validated
umi GAAACCAGCA = 1940 reads: +415 validated

UMI info for barcode ACGCCAGCACGAGAGT-1 contig 2 = GACTAGGGAT...
umi ACGCGGCCAA = 1195 reads: +400 validated
umi AGGCTGTTCA = 1911 reads: +400 validated
umi CTGCGTCCGC = 173 reads: -375 +5 -1XX +1 -2XX +2 -4XX +2 -4XX +2 -2XX invalidated
umi TATACTCCCC = 339 reads: +370 -4 +26 non-validated

GOOD CONTIGS

TIG 1[bases=487]
0-20 ==> 0-20 on |612|TRBV28|5'UTR| [len=20] (mis=0)
20-364 ==> 0-344 on |613|TRBV28|L-REGION+V-REGION| [len=344] (mis=5)
366-378 ==> 0-12 on |535|TRBD1|D-REGION| [len=12] (mis=3)
382-435 ==> 0-53 on |549|TRBJ2-6|J-REGION| [len=53] (mis=1)
435-487 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 4 umis using 367 reads
cdr3 = CASRESPDRGWVPGANVLTF at 347, score = 6 + 10
umis assigned: [190, 281, 381, 567, 607]
of which 5 are surviving nonsolos
reads assigned: 6768
start codons at 20, 78, 142, 155, 162, 170, 225, 228, 236, 338
confident = true

TIG 2[bases=533]
0-91 ==> 56-147 on |470|TRAV17|5'UTR| [len=147] (mis=0)
91-428 ==> 0-337 on |471|TRAV17|L-REGION+V-REGION| [len=337] (mis=1)
428-491 ==> 0-63 on |398|TRAJ13|J-REGION| [len=63] (mis=4)
491-533 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 2 umis using 160 reads
cdr3 = CATDNYSGGYQKVTF at 415, score = 6 + 8
umis assigned: [121, 185, 585, 835]
of which 4 are surviving nonsolos
reads assigned: 3548
start codons at 91, 126, 203, 211, 305
confident = true
now this is a cell
paired!

GCAGCAGACACTGCTTCTTACTTCTGTGCTACGGACAACTATTCTGGGGGTTACCAGAAAGTTACCTTTGGAATTGGAACAAAGCTCCAAGTCATCCCAA <==> TCTATGTACCTCTGTGCCAGCAGAGAGTCGCCAGACAGGGGGTGGGTCCCTGGGGCCAACGTCCTGACTTTCGGGGCCGGCAGCAGGCTGACCGTGCTGG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.220 = ACGCCAGCAGAAGCAC-1

using 61 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[61]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.222 = ACGCCAGCAGACACTT-1

using 61 reads

====================================================================================

graph has 78 edges initially, 4 edges after simplification

total ucounts = 15
nonsolo ucounts = 8[2^2, 4, 6, 8^2, 10, 14]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.224 = ACGCCAGCAGACGTAG-1

using 446 reads

====================================================================================

graph has 308 edges initially, 10 edges after simplification

total ucounts = 19
nonsolo ucounts = 16[2, 3^2, 5, 8^2, 9, 10, 11, 40^2, 43, 47, 51, 79, 84]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.228 = ACGCCAGCAGCTGTAT-1

using 80 reads

====================================================================================

graph has 110 edges initially, 10 edges after simplification

total ucounts = 19
nonsolo ucounts = 13[2^3, 3^2, 4, 5^2, 6, 8, 9, 10, 15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.233 = ACGCCAGCAGTCACTA-1

using 113 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[113]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.236 = ACGCCAGCAGTTTACG-1

using 45 reads

====================================================================================

graph has 64 edges initially, 2 edges after simplification

total ucounts = 15
nonsolo ucounts = 11[2^3, 3^3, 4^2, 5, 6, 7]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.239 = ACGCCAGCATCCCACT-1

using 462 reads

====================================================================================

graph has 546 edges initially, 14 edges after simplification

total ucounts = 33
nonsolo ucounts = 25[2^3, 3^4, 5^2, 6^2, 7, 9, 10, 12^2, 16, 17, 20, 27, 28, 38, 47, 71, 100]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.247 = ACGCCAGCATGGTCAT-1

using 79 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[79]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.248 = ACGCCAGCATTACCTT-1

using 94 reads

====================================================================================

graph has 72 edges initially, 2 edges after simplification

total ucounts = 13
nonsolo ucounts = 6[2, 3, 4, 6, 8, 64]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.251 = ACGCCAGCATTCCTGC-1

using 125 reads

====================================================================================

graph has 94 edges initially, 4 edges after simplification

total ucounts = 13
nonsolo ucounts = 10[4, 5^2, 6, 8, 15, 16, 17, 19, 27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.253 = ACGCCAGCATTTGCCC-1

using 52 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[51]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.257 = ACGCCAGGTACGAAAT-1

using 34 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.259 = ACGCCAGGTAGCTAAA-1

using 41 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[40]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.264 = ACGCCAGGTATGAAAC-1

using 29 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.267 = ACGCCAGGTCAAAGAT-1

using 73 reads

====================================================================================

graph has 64 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 7[3, 5, 6^3, 13, 32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.277 = ACGCCAGGTCATGCAT-1

using 48 reads

====================================================================================

graph has 34 edges initially, 4 edges after simplification

total ucounts = 7
nonsolo ucounts = 5[2^2, 4, 19^2]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.279 = ACGCCAGGTCCGAATT-1

using 36 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.285 = ACGCCAGGTCTCAACA-1

using 1926 reads

====================================================================================

graph has 584 edges initially, 8 edges after simplification

total ucounts = 24
nonsolo ucounts = 17[2^4, 3, 5, 8, 10, 11, 14, 16, 21, 35, 50, 59, 95, 1584]
surviving nonsolo ucounts = 1[1584]
ids = [6]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.287 = ACGCCAGGTCTGATTG-1

using 41 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[41]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.298 = ACGCCAGGTTCACCTC-1

using 13 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[12]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.304 = ACGCCAGGTTTAGCTG-1

using 50 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[50]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.305 = ACGCCAGGTTTGTTTC-1

using 122 reads

====================================================================================

graph has 136 edges initially, 4 edges after simplification

total ucounts = 23
nonsolo ucounts = 18[2^3, 3^2, 4^3, 5^2, 6, 7^2, 8, 10, 11, 12, 22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.310 = ACGCCAGTCAAGCCTA-1

using 36 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.312 = ACGCCAGTCACATACG-1

using 83 reads

====================================================================================

graph has 84 edges initially, 4 edges after simplification

total ucounts = 20
nonsolo ucounts = 11[3^2, 4^4, 5, 7, 8, 15, 17]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.314 = ACGCCAGTCAGCACAT-1

using 44 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[44]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.319 = ACGCCAGTCAGTGTTG-1

using 738 reads

====================================================================================

graph has 292 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[2, 736]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.320 = ACGCCAGTCATACGGT-1

using 27 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 5[2^2, 3, 4, 13]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.325 = ACGCCAGTCATGTCTT-1

using 48 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[48]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.328 = ACGCCAGTCCAAGTAC-1

using 106 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[106]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.330 = ACGCCAGTCCACGACG-1

using 69 reads

====================================================================================

graph has 90 edges initially, 2 edges after simplification

total ucounts = 21
nonsolo ucounts = 12[2^5, 3, 4^2, 6, 7, 9, 17]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.335 = ACGCCAGTCCCAACGG-1

using 27 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.337 = ACGCCAGTCCCTGACT-1

using 54 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[54]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.338 = ACGCCAGTCCGAACGC-1

using 39 reads

====================================================================================

graph has 48 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 4[2, 4, 9, 18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.339 = ACGCCAGTCCGCAGTG-1

using 2345 reads

====================================================================================

graph has 758 edges initially, 4 edges after simplification

total ucounts = 15
nonsolo ucounts = 9[2, 4, 5^2, 7, 9^2, 10, 2288]
surviving nonsolo ucounts = 1[2288]
ids = [14]

====================================================================================

UMI info for barcode ACGCCAGTCCGCAGTG-1 contig 1 = ACAACTCTCC...
umi TGACGGGTTT = 2316 reads: +391 validated

GOOD CONTIGS

TIG 1[bases=485]
0-42 ==> 36-78 on |597|TRBV20-1|5'UTR| [len=78] (mis=0)
42-377 ==> 0-335 on |598|TRBV20-1|L-REGION+V-REGION| [len=335] (mis=1)
385-433 ==> 0-48 on |548|TRBJ2-5|J-REGION| [len=48] (mis=2)
433-485 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 1 umis using 169 reads
cdr3 = CSARHPEEEETQYF at 363, score = 7 + 7
umis assigned: [14]
of which 1 are surviving nonsolos
reads assigned: 2266
start codons at 42, 180, 216, 222, 235, 295
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.342 = ACGCCAGTCCTAAGTG-1

using 45 reads

====================================================================================

graph has 52 edges initially, 2 edges after simplification

total ucounts = 13
nonsolo ucounts = 6[2^2, 5, 6, 7, 16]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.343 = ACGCCAGTCCTCAATT-1

using 44 reads

====================================================================================

graph has 82 edges initially, 2 edges after simplification

total ucounts = 15
nonsolo ucounts = 10[2^5, 3^2, 4, 9, 10]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.345 = ACGCCAGTCCTGTACC-1

using 59 reads

====================================================================================

graph has 62 edges initially, 2 edges after simplification

total ucounts = 14
nonsolo ucounts = 7[2^2, 4^2, 6, 7, 27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.349 = ACGCCAGTCGATAGAA-1

using 237 reads

====================================================================================

graph has 148 edges initially, 6 edges after simplification

total ucounts = 14
nonsolo ucounts = 11[2, 3, 4^2, 5, 8, 25^2, 44, 53, 61]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.358 = ACGCCAGTCTAACTCT-1

using 32 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.359 = ACGCCAGTCTAAGCCA-1

using 147 reads

====================================================================================

graph has 194 edges initially, 2 edges after simplification

total ucounts = 24
nonsolo ucounts = 16[2, 3^2, 4^2, 5^3, 6, 9^4, 16, 18, 32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.361 = ACGCCAGTCTACTCAT-1

using 89 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[89]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.362 = ACGCCAGTCTAGAGTC-1

using 7370 reads

====================================================================================

graph has 5491 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 1[7367]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.363 = ACGCCAGTCTCATTCA-1

using 35 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.368 = ACGCCAGTCTGATTCT-1

using 37 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.372 = ACGCCAGTCTGTGCAA-1

using 5066 reads

====================================================================================

graph has 3563 edges initially, 130 edges after simplification

total ucounts = 871
nonsolo ucounts = 616[2^122, 3^81, 4^61, 5^57, 6^42, 7^37, 8^31, 9^29, 10^21, 11^22, 12^12, 13^10, 14^16, 15^12, 16^6, 17^8, 18^5, 19^6, 20^5, 21^3, 22^2, 23, 24^3, 25^4, 26^4, 27, 28, 29, 31, 32, 35, 36^2, 37, 38^3, 57, 74, 79, 88]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.373 = ACGCCAGTCTGTTGAG-1

using 12 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[12]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.378 = ACGCCAGTCTTGTACT-1

using 42 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 2[3, 36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.379 = ACGCCAGTCTTGTATC-1

using 39 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[39]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.380 = ACGCCAGTCTTTACGT-1

using 28 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.381 = ACGCCAGTCTTTAGTC-1

using 30 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.385 = ACGCCGAAGACACTAA-1

using 28 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.388 = ACGCCGAAGATAGGAG-1

using 4688 reads

====================================================================================

graph has 1280 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 5[2, 3, 4, 13, 4661]
surviving nonsolo ucounts = 1[4661]
ids = [5]

====================================================================================

REJECT CONTIGS

TIG 1[bases=321]
8-208 ==> 5800-6000 on segment before TRBD1 exon 1 [len=6000] (mis=0)
219-269 ==> 0-50 on |543|TRBJ2-1|J-REGION| [len=50] (mis=3)
269-321 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
umis assigned: [5]
of which 1 are surviving nonsolos
reads assigned: 4596
start codons at 85, 95, 169, 227
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.391 = ACGCCGAAGCAATATG-1

using 59 reads

====================================================================================

graph has 80 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 5[2, 3, 15, 17, 21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.392 = ACGCCGAAGCCACGTC-1

using 34 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.393 = ACGCCGAAGCCGGTAA-1

using 31 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[2, 6, 21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.401 = ACGCCGAAGCTGTCTA-1

using 92 reads

====================================================================================

graph has 122 edges initially, 4 edges after simplification

total ucounts = 8
nonsolo ucounts = 5[2, 11, 17, 22, 37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.402 = ACGCCGAAGCTTTGGT-1

using 2033 reads

====================================================================================

graph has 2439 edges initially, 53 edges after simplification

total ucounts = 353
nonsolo ucounts = 228[2^53, 3^29, 4^18, 5^19, 6^16, 7^12, 8^13, 9^6, 10^5, 11^4, 12^10, 13^7, 14^3, 15^2, 16^2, 17^5, 18^3, 19^4, 20^2, 21, 22, 23, 28, 29, 30, 32, 33, 36, 37, 39, 49, 50, 54, 63]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.404 = ACGCCGAAGGAGTACC-1

using 24 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[3, 5, 14]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.406 = ACGCCGAAGGATGTAT-1

using 74 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[74]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.407 = ACGCCGAAGGGTGTTG-1

using 26 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.408 = ACGCCGAAGGGTTTCT-1

using 88 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[88]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.409 = ACGCCGAAGGTGCAAC-1

using 40 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[40]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.418 = ACGCCGAAGTTACGGG-1

using 40 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[40]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.420 = ACGCCGACAAACAACA-1

using 40 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[2, 11, 25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.423 = ACGCCGACAATCGGTT-1

using 52 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 5[5, 6, 12, 13, 15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.429 = ACGCCGACACACTGCG-1

using 2864 reads

====================================================================================

graph has 938 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 3[6, 27, 2827]
surviving nonsolo ucounts = 1[2827]
ids = [4]

====================================================================================

REJECT CONTIGS

TIG 1[bases=449]
19-316 ==> 46-343 on |631|TRBV5-1|L-REGION+V-REGION| [len=343] (mis=0)
347-397 ==> 0-50 on |543|TRBJ2-1|J-REGION| [len=50] (mis=3)
397-449 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
cdr3 = CASSLGGREEGNRPILLYNEQFF at 300, score = 9 + 8
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 2789
start codons at 255, 259, 355
confident = false
not full
VJ delta = 18
not full
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.430 = ACGCCGACACAGAGGT-1

using 48 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[48]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.431 = ACGCCGACACAGGAGT-1

using 69054 reads

====================================================================================

graph has 17670 edges initially, 156 edges after simplification

total ucounts = 990
nonsolo ucounts = 668[2^134, 3^82, 4^67, 5^58, 6^53, 7^39, 8^30, 9^32, 10^22, 11^18, 12^17, 13^18, 14^9, 15^13, 16^7, 17^8, 18^6, 19^4, 21^4, 22^2, 23^4, 24, 26, 27, 28, 29^2, 31^3, 32^2, 37^2, 39, 48, 49, 52, 55, 110, 120, 157, 219, 422, 472, 599, 1246, 1641, 1646, 1714, 2257, 2391, 2448, 2616, 2625, 2946, 2995, 3276, 6269, 8660, 9493, 9753]
surviving nonsolo ucounts = 18[472, 599, 1246, 1641, 1646, 1714, 2257, 2391, 2448, 2616, 2625, 2946, 2995, 3276, 6269, 8660, 9493, 9753]
ids = [40, 766, 600, 656, 722, 881, 507, 147, 180, 611, ...]

====================================================================================

UMI info for barcode ACGCCGACACAGGAGT-1 contig 1 = AGCCCCAAGC...
umi AGCACCGTTG = 3011 reads: +394 validated
umi AGCTGGTGCT = 2408 reads: +394 validated
umi AGTTATACAA = 2455 reads: +394 validated
umi CTGACGGGCA = 2265 reads: +394 validated
umi GCGGGATCGA = 2635 reads: +394 validated
umi GTACATTTAA = 2963 reads: +394 validated
umi GTACTTCCGT = 3283 reads: +394 validated
umi TAAAACGGTG = 1640 reads: +394 validated
umi TATATATGTT = 593 reads: +380 -1X +13 invalidated
umi TGATAATGGG = 1704 reads: +394 validated
umi TTCTCTAGTC = 2648 reads: +394 validated

UMI info for barcode ACGCCGACACAGGAGT-1 contig 2 = GCAAGTCTTT...
umi GCCGCGCAAC = 1248 reads: +391 validated
umi GGGTCCTGAG = 1651 reads: +391 validated

GOOD CONTIGS

TIG 1[bases=470]
0-24 ==> 23-47 on |692|TRBV9|5'UTR| [len=47] (mis=0)
24-367 ==> 0-343 on |693|TRBV9|L-REGION+V-REGION| [len=343] (mis=2)
367-418 ==> 0-51 on |544|TRBJ2-2|J-REGION| [len=51] (mis=5)
418-470 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 11 umis using 1853 reads
cdr3 = CASSVQPGGELFF at 351, score = 9 + 9
umis assigned: [138, 147, 180, 507, 611, 680, 685, 722, 766, 881] and 1 others
of which 11 are surviving nonsolos
reads assigned: 25104
start codons at 24, 146, 232
confident = true

TIG 2[bases=542]
0-109 ==> 100-209 on |489|TRAV26-1|5'UTR| [len=209] (mis=0)
109-437 ==> 0-328 on |490|TRAV26-1|L-REGION+V-REGION| [len=328] (mis=1)
438-500 ==> 0-62 on |423|TRAJ37|J-REGION| [len=62] (mis=2)
500-542 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 2 umis using 133 reads
cdr3 = CIVRVAGSGNTGKLIF at 421, score = 8 + 8
umis assigned: [600, 656]
of which 2 are surviving nonsolos
reads assigned: 2849
start codons at 109, 161, 187, 248, 254, 305, 320, 329, 334
confident = true

REJECT CONTIGS

TIG 1[bases=351]
5-238 ==> 5767-6000 on segment before TRBD1 exon 1 [len=6000] (mis=0)
249-299 ==> 0-50 on |540|TRBJ1-5|J-REGION| [len=50] (mis=3)
299-351 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
umis assigned: [40, 126, 747, 793, 908]
of which 5 are surviving nonsolos
reads assigned: 34178
start codons at 115, 125, 199, 275
confident = false
did not find CDR3
now this is a cell
paired!

AGAGACACTGCTGTGTACTATTGCATCGTCAGAGTCGCCGGCTCTGGCAACACAGGCAAACTAATCTTTGGGCAAGGGACAACTTTACAAGTAAAACCAG <==> TCTCTGGAGCTGGGGGACTCAGCTTTGTATTTCTGTGCCAGCAGCGTCCAGCCCGGCGGGGAGCTGTTTTTTGGAGAAGGCTCTAGGCTGACCGTACTGG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.436 = ACGCCGACACGAAATA-1

using 51 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[51]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.440 = ACGCCGACACTCGACG-1

using 41 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[41]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.447 = ACGCCGACAGCCTATA-1

using 43 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[2, 41]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.448 = ACGCCGACAGCGATCC-1

using 82 reads

====================================================================================

graph has 48 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[4, 78]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.449 = ACGCCGACAGCTGCTG-1

using 987 reads

====================================================================================

graph has 932 edges initially, 32 edges after simplification

total ucounts = 210
nonsolo ucounts = 98[2^24, 3^16, 4^12, 5^10, 6^3, 7^4, 8^3, 9^2, 10^2, 11^2, 12, 13^3, 14^2, 15, 17^2, 18, 19, 24, 29^3, 37, 38, 39, 60, 101]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.451 = ACGCCGACAGGGAGAG-1

using 29 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.457 = ACGCCGACATACGCCG-1

using 54 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[5, 48]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.459 = ACGCCGACATATGCTG-1

using 52 reads

====================================================================================

graph has 78 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 6[2^2, 4, 9, 12, 19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.460 = ACGCCGACATCACCCT-1

using 39 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[5, 12, 20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.465 = ACGCCGACATCTACGA-1

using 403 reads

====================================================================================

graph has 228 edges initially, 12 edges after simplification

total ucounts = 19
nonsolo ucounts = 17[3^5, 4, 5, 6, 9, 10, 19, 29, 43, 45, 46, 66, 104]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.467 = ACGCCGACATGCAACT-1

using 49 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 3[2^2, 45]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.469 = ACGCCGACATTACCTT-1

using 52 reads

====================================================================================

graph has 74 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 5[3, 4^2, 14, 24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.471 = ACGCCGAGTAAGCACG-1

using 15 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[14]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.472 = ACGCCGAGTAAGTTCC-1

using 63 reads

====================================================================================

graph has 54 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[63]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.476 = ACGCCGAGTATAATGG-1

using 111 reads

====================================================================================

graph has 112 edges initially, 2 edges after simplification

total ucounts = 16
nonsolo ucounts = 9[4^3, 5, 7, 10, 12, 19, 39]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.478 = ACGCCGAGTCAGTGGA-1

using 52 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[52]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.483 = ACGCCGAGTCCCTACT-1

using 169 reads

====================================================================================

graph has 158 edges initially, 2 edges after simplification

total ucounts = 14
nonsolo ucounts = 12[3, 6, 7, 8, 9, 10, 12, 14^2, 19, 22, 43]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.501 = ACGCCGAGTGGGTCAA-1

using 81 reads

====================================================================================

graph has 70 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[81]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.517 = ACGCCGATCAGCCTAA-1

using 46 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[46]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.518 = ACGCCGATCAGTTCGA-1

using 37 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 5[2^2, 3, 5, 21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.521 = ACGCCGATCATTTGGG-1

using 130 reads

====================================================================================

graph has 124 edges initially, 4 edges after simplification

total ucounts = 17
nonsolo ucounts = 13[2^3, 4, 5^2, 7, 8, 11, 12, 15, 20, 33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.526 = ACGCCGATCCCTCTTT-1

using 66 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[65]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.534 = ACGCCGATCGGTGTCG-1

using 78 reads

====================================================================================

graph has 112 edges initially, 4 edges after simplification

total ucounts = 17
nonsolo ucounts = 12[2^5, 3^2, 4, 5, 11, 14, 23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.536 = ACGCCGATCGTTTAGG-1

using 471 reads

====================================================================================

graph has 252 edges initially, 2 edges after simplification

total ucounts = 14
nonsolo ucounts = 10[2, 3^2, 4^3, 5^2, 17, 420]
surviving nonsolo ucounts = 1[420]
ids = [0]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.540 = ACGCCGATCTGCAGTA-1

using 70 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[70]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.546 = ACGCCGATCTTCATGT-1

using 79 reads

====================================================================================

graph has 132 edges initially, 2 edges after simplification

total ucounts = 12
nonsolo ucounts = 11[2, 3, 4^2, 5, 7, 8, 10^2, 12, 13]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.547 = ACGCCGATCTTGGGTA-1

using 63 reads

====================================================================================

graph has 58 edges initially, 4 edges after simplification

total ucounts = 10
nonsolo ucounts = 5[2, 3, 9, 16, 28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.550 = ACGGAGAAGACAAAGG-1

using 30 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.551 = ACGGAGAAGACCGGAT-1

using 51 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[7, 44]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.559 = ACGGAGAAGCCGGTAA-1

using 224 reads

====================================================================================

graph has 180 edges initially, 4 edges after simplification

total ucounts = 12
nonsolo ucounts = 7[2, 9, 18, 22, 25, 60, 83]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.560 = ACGGAGAAGCGACGTA-1

using 30 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[5, 7, 16]
surviving nonsolo ucounts = 1[16]
ids = [2]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.564 = ACGGAGAAGGCTCAGA-1

using 27776 reads

====================================================================================

graph has 8285 edges initially, 148 edges after simplification

total ucounts = 460
nonsolo ucounts = 347[2^45, 3^31, 4^20, 5^14, 6^25, 7^17, 8^14, 9^19, 10^11, 11^12, 12^21, 13^11, 14^10, 15^7, 16^6, 17^8, 18^5, 19^8, 20^8, 21^8, 22^3, 23^3, 24^3, 25^4, 26^2, 27, 28, 30^2, 31^2, 33, 34, 35, 36, 37, 38, 41^2, 48^2, 53, 73, 75, 80, 82, 84, 86, 87, 117, 125, 253, 294, 497, 1252, 10493, 10516]
surviving nonsolo ucounts = 8[75, 86, 253, 294, 497, 1252, 10493, 10516]
ids = [142, 452, 313, 294, 173, 160, 41, 158]

====================================================================================

UMI info for barcode ACGGAGAAGGCTCAGA-1 contig 1 = GCCAGGTTCA...
umi CCCAGTGAAA = 64 reads: -376 +2 -3X +2 -1X +4 -2XX +2 -1XX +1 -1XX +3 -2XX +1 -1XX +2 -2XX invalidated
umi CCGCTCATCC = 1237 reads: +406 validated
umi GTTATCATTC = 225 reads: -374 +1 -2 +1 -3X +2 -1XX +4 -2XX +2 -1XX +1 -1XX +3 -2XX +1 -1XX +2 -2XX invalidated

GOOD CONTIGS

TIG 1[bases=523]
0-75 ==> 0-75 on |467|TRAV14/DV4|5'UTR| [len=75] (mis=3)
75-425 ==> 0-350 on |468|TRAV14/DV4|L-REGION+V-REGION| [len=350] (mis=7)
426-481 ==> 0-55 on |406|TRAJ21|J-REGION| [len=55] (mis=0)
481-523 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 1 umis using 50 reads
cdr3 = CAMRALYNFNKFYF at 408, score = 7 + 7
umis assigned: [142, 160, 313]
of which 3 are surviving nonsolos
reads assigned: 1508
start codons at 75, 165, 208, 229, 267, 295, 307, 399, 414, 469
confident = true

REJECT CONTIGS

TIG 1[bases=307]
5-200 ==> 5805-6000 on segment before TRBD1 exon 1 [len=6000] (mis=0)
207-255 ==> 0-48 on |537|TRBJ1-2|J-REGION| [len=48] (mis=0)
255-307 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
umis assigned: [41, 158, 294, 442]
of which 3 are surviving nonsolos
reads assigned: 19553
start codons at 77, 87, 161, 213
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.565 = ACGGAGAAGGTGATAT-1

using 88 reads

====================================================================================

graph has 64 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 5[2, 7, 8, 11, 58]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.570 = ACGGAGAAGTCAATAG-1

using 90 reads

====================================================================================

graph has 38 edges initially, 4 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[5, 40, 43]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.571 = ACGGAGAAGTCATGCT-1

using 13 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[13]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.572 = ACGGAGAAGTCGTTTG-1

using 18 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[17]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.576 = ACGGAGACAACGATGG-1

using 59 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[59]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.578 = ACGGAGACAAGCCATT-1

using 48 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[48]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.580 = ACGGAGACAAGTAATG-1

using 80000 reads

====================================================================================

graph has 43379 edges initially, 1582 edges after simplification

total ucounts = 3635
nonsolo ucounts = 3077[2^314, 3^237, 4^201, 5^125, 6^103, 7^97, 8^91, 9^75, 10^58, 11^73, 12^66, 13^58, 14^61, 15^41, 16^51, 17^48, 18^28, 19^33, 20^38, 21^32, 22^33, 23^30, 24^31, 25^34, 26^28, 27^26, 28^32, 29^32, 30^31, 31^25, 32^24, 33^16, 34^24, 35^26, 36^20, 37^12, 38^18, 39^19, 40^20, 41^18, 42^20, 43^15, 44^20, 45^27, 46^18, 47^16, 48^14, 49^22, 50^18, 51^16, 52^21, 53^21, 54^21, 55^22, 56^13, 57^10, 58^14, 59^16, 60^15, 61^22, 62^13, 63^15, 64^12, 65^15, 66^8, 67^10, 68^12, 69^17, 70^9, 71^9, 72^12, 73^13, 74^11, 75^11, 76^10, 77^7, 78^14, 79^10, 80^6, 81^11, 82^5, 83^6, 84^5, 85^13, 86^9, 87^14, 88^5, 89^5, 90^4, 91^6, 92^7, 93^2, 94^5, 95^6, 96^6, 97^2, 98^3, 99, 100^5, 101^6, 102, 103^2, 104^4, 106, 107, 108, 110^8, 111^4, 112^3, 113^5, 114^2, 115^2, 117, 120, 121, 124, 125, 126, 127, 131, 134, 148, 160^2, 162]
surviving nonsolo ucounts = 4[5, 10, 43, 59]
ids = [2359, 2150, 3573, 3211]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.586 = ACGGAGACAATGTAAG-1

using 46 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[46]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.588 = ACGGAGACACGAGAGT-1

using 237 reads

====================================================================================

graph has 164 edges initially, 4 edges after simplification

total ucounts = 7
nonsolo ucounts = 7[2, 4, 16^2, 22, 84, 93]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.589 = ACGGAGACACGTGAGA-1

using 56 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 2[2, 51]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.591 = ACGGAGACAGCTCGCA-1

using 38 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.593 = ACGGAGACAGGAATGC-1

using 54 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[53]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.594 = ACGGAGACAGGTCGTC-1

using 2485 reads

====================================================================================

graph has 730 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 3[2, 6, 2472]
surviving nonsolo ucounts = 1[2472]
ids = [5]

====================================================================================

UMI info for barcode ACGGAGACAGGTCGTC-1 contig 1 = TGGGGAATTG...
umi AAGCGCTTTC = 2474 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=458]
22-359 ==> 0-337 on |464|TRAV13-1|L-REGION+V-REGION| [len=337] (mis=3)
362-416 ==> 6-60 on |441|TRAJ54|J-REGION| [len=60] (mis=0)
416-458 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 1 umis using 130 reads
cdr3 = CAASGKGAQKLVF at 346, score = 9 + 9
umis assigned: [5]
of which 1 are surviving nonsolos
reads assigned: 2431
start codons at 22, 77, 86, 236
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.595 = ACGGAGACAGGTTTCA-1

using 2369 reads

====================================================================================

graph has 598 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[2369]
surviving nonsolo ucounts = 1[2369]
ids = [0]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.604 = ACGGAGACATGGTCTA-1

using 14 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[14]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.606 = ACGGAGACATGTAGTC-1

using 76 reads

====================================================================================

graph has 104 edges initially, 4 edges after simplification

total ucounts = 11
nonsolo ucounts = 7[3^3, 4, 6, 12, 41]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.608 = ACGGAGACATTAGGCT-1

using 163 reads

====================================================================================

graph has 66 edges initially, 4 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[27, 136]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.619 = ACGGAGAGTATCTGCA-1

using 628 reads

====================================================================================

graph has 434 edges initially, 4 edges after simplification

total ucounts = 43
nonsolo ucounts = 30[3^5, 4, 5, 6^3, 7^3, 9, 12^3, 13, 14^2, 18^2, 24, 33, 34, 51^2, 73, 74, 90]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.620 = ACGGAGAGTATGAATG-1

using 34 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.626 = ACGGAGAGTCTCGTTC-1

using 31 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.639 = ACGGAGATCACCCGAG-1

using 93 reads

====================================================================================

graph has 48 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[93]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.651 = ACGGAGATCCAAGCCG-1

using 13 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[13]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.654 = ACGGAGATCCCGGATG-1

using 81 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 4[2^2, 3, 74]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.665 = ACGGAGATCGCCAAAT-1

using 274 reads

====================================================================================

graph has 96 edges initially, 4 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[43, 229]
surviving nonsolo ucounts = 1[229]
ids = [1]

====================================================================================

UMI info for barcode ACGGAGATCGCCAAAT-1 contig 1 = GATCCTTCCC...
umi ACATCATGTC = 144 reads: +397 validated

GOOD CONTIGS

TIG 1[bases=417]
20-362 ==> 0-342 on |733|TRBV11-2|L-REGION+V-REGION| [len=342] (mis=2)
369-417 ==> 0-48 on |537|TRBJ1-2|J-REGION| [len=48] (mis=5)
junction support: 1 umis using 24 reads
cdr3 = CASTLPGEDGYTF at 350, score = 8 + 9
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 141
start codons at 20, 162, 243, 375
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.671 = ACGGAGATCTCAACTT-1

using 80 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[80]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.677 = ACGGCCAAGAAACCAT-1

using 24 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.678 = ACGGCCAAGAAGATTC-1

using 69 reads

====================================================================================

graph has 88 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 7[3^2, 7, 9, 10, 16, 20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.685 = ACGGCCAAGATCACGG-1

using 3091 reads

====================================================================================

graph has 1102 edges initially, 4 edges after simplification

total ucounts = 11
nonsolo ucounts = 7[3, 4, 5, 6, 7, 33, 3029]
surviving nonsolo ucounts = 1[3029]
ids = [9]

====================================================================================

UMI info for barcode ACGGCCAAGATCACGG-1 contig 1 = TGGGGATAGC...
umi TGTGAACATT = 3049 reads: +397 validated

GOOD CONTIGS

TIG 1[bases=455]
16-353 ==> 0-337 on |500|TRAV34|L-REGION+V-REGION| [len=337] (mis=5)
354-413 ==> 7-66 on |432|TRAJ45|J-REGION| [len=66] (mis=0)
413-455 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 1 umis using 191 reads
cdr3 = CGPGRGGGADGLTF at 340, score = 7 + 8
umis assigned: [9]
of which 1 are surviving nonsolos
reads assigned: 2986
start codons at 16, 164, 191, 214, 217, 248, 272, 323
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.688 = ACGGCCAAGCAGACTG-1

using 73 reads

====================================================================================

graph has 76 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 6[2^3, 3, 4, 59]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.690 = ACGGCCAAGCAGCGTA-1

using 517 reads

====================================================================================

graph has 264 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 6[2^2, 4, 6, 8, 492]
surviving nonsolo ucounts = 1[492]
ids = [3]

====================================================================================

REJECT CONTIGS

TIG 1[bases=345]
17-243 ==> 108-334 on |521|TRAV8-2|L-REGION+V-REGION| [len=341] (mis=1)
248-303 ==> 8-63 on |435|TRAJ48|J-REGION| [len=63] (mis=0)
303-345 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
cdr3 = CVAVFGNEKLTF at 236, score = 7 + 10
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 483
start codons at 72, 212, 255
confident = false
VJ delta = 26
not full
not full
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.691 = ACGGCCAAGCATCATC-1

using 14 reads

====================================================================================

graph has 9 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[14]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.693 = ACGGCCAAGCCCAATT-1

using 143 reads

====================================================================================

graph has 136 edges initially, 4 edges after simplification

total ucounts = 16
nonsolo ucounts = 11[2^2, 3, 4, 5, 6, 7, 12, 13, 17, 67]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.697 = ACGGCCAAGCGTGAGT-1

using 5691 reads

====================================================================================

graph has 1028 edges initially, 4 edges after simplification

total ucounts = 9
nonsolo ucounts = 6[2, 3^2, 5, 82, 5593]
surviving nonsolo ucounts = 1[5593]
ids = [6]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.700 = ACGGCCAAGGACAGCT-1

using 33 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.701 = ACGGCCAAGGACTGGT-1

using 31 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.704 = ACGGCCAAGGGTGTGT-1

using 33 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 4[2^2, 12, 13]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.709 = ACGGCCAAGTATGACA-1

using 424 reads

====================================================================================

graph has 402 edges initially, 18 edges after simplification

total ucounts = 19
nonsolo ucounts = 16[2^4, 3, 4, 5^2, 9, 25, 33, 35, 51, 58, 64, 121]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.711 = ACGGCCAAGTCAAGGC-1

using 24 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 1[22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.712 = ACGGCCAAGTCATGCT-1

using 125 reads

====================================================================================

graph has 90 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 8[3^3, 5, 7, 13, 20, 68]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.716 = ACGGCCAAGTGAAGAG-1

using 54 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[14, 40]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.722 = ACGGCCACAACACCTA-1

using 72 reads

====================================================================================

graph has 76 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[14, 57]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.725 = ACGGCCACAAGCGTAG-1

using 98 reads

====================================================================================

graph has 54 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[98]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.726 = ACGGCCACAAGCTGTT-1

using 202 reads

====================================================================================

graph has 156 edges initially, 14 edges after simplification

total ucounts = 17
nonsolo ucounts = 11[2, 3^2, 4, 5^2, 6, 13, 45, 52, 58]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.730 = ACGGCCACACAGCCCA-1

using 111 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[111]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.736 = ACGGCCACACGAAAGC-1

using 57 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[57]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.742 = ACGGCCACAGACAAAT-1

using 66 reads

====================================================================================

graph has 98 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 8[2^4, 4^2, 15, 34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.743 = ACGGCCACAGACACTT-1

using 23 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.745 = ACGGCCACAGCCACCA-1

using 54694 reads

====================================================================================

graph has 14505 edges initially, 86 edges after simplification

total ucounts = 446
nonsolo ucounts = 253[2^60, 3^41, 4^17, 5^14, 6^15, 7^8, 8^7, 9^8, 10^7, 11^2, 12^3, 13^7, 14^4, 15^4, 16^6, 18^3, 19^2, 20, 22, 23^2, 24, 25, 26, 27, 29, 30^2, 33^2, 35, 37, 38, 39, 43, 45, 48^2, 55, 57, 73, 79, 154, 603, 896, 905, 1300, 1419, 1420, 1552, 1774, 1808, 2229, 2368, 2503, 2554, 2652, 2722, 2863, 6943, 7452, 8264]
surviving nonsolo ucounts = 18[603, 905, 1300, 1419, 1420, 1552, 1774, 1808, 2229, 2368, 2503, 2554, 2652, 2722, 2863, 6943, 7452, 8264]
ids = [420, 213, 171, 2, 19, 206, 249, 367, 292, 4, ...]

====================================================================================

UMI info for barcode ACGGCCACAGCCACCA-1 contig 1 = ATCACTTGAA...
umi AAAAGCTGTG = 1416 reads: +400 validated
umi AAACGTGCCT = 2311 reads: -268 +132 non-validated
umi AAGAGCTCGG = 1287 reads: -381X +2 -2XX +1 -1XX +3 -1XX +1 -2XX +2 -2XX +2 invalidated
umi ATGAAAATGA = 2646 reads: +400 validated
umi CACCGTACCA = 2509 reads: +400 validated
umi CCCTGTCTCG = 7424 reads: -242X +1 -3XX +154 invalidated
umi CGTCGGTACC = 1550 reads: +400 validated
umi CGTTGGTTAT = 909 reads: +400 validated
umi CTTTCTCGAC = 2867 reads: +400 validated
umi GACGTACGTG = 1767 reads: +400 validated
umi GCTAGTACAT = 2691 reads: +400 validated
umi TCAATGCGTC = 6977 reads: -216X +184 invalidated
umi TCAGCGGTAA = 1797 reads: +400 validated
umi TTAGGCCCTT = 533 reads: -385 +2 -5XX +1 -5XX +2 invalidated

UMI info for barcode ACGGCCACAGCCACCA-1 contig 2 = CTTCTGCAGA...
umi ACCACGATGC = 2548 reads: +223 -1XX +1 -10XX +1 -3XX +2 -1XX +1 -6XX +1 -3XX +1 -100X +1 -11XX +2 -1XX +1 -3XX +1 -2XX +2 -1XX invalidated
umi CCCGTGGGCC = 1292 reads: +379 validated
umi GGCCGTGTCA = 2244 reads: +379 validated
umi TACCTTTCGG = 8383 reads: +116 -2XX +1 -5XX +1 -3XX +1 -6XX +1 -119XX +1 -3XX +1 -4XX +1 -4XX +1 -5XX +1 -3XX +100 invalidated
umi TCTAGTTCGA = 1 reads: -369X +1 -2X +2 -5X invalidated

GOOD CONTIGS

TIG 1[bases=463]
11-346 ==> 0-335 on |615|TRBV29-1|L-REGION+V-REGION| [len=335] (mis=1)
353-369 ==> 0-16 on |730|TRBD2|D-REGION| [len=16] (mis=3)
364-411 ==> 0-47 on |550|TRBJ2-7|J-REGION| [len=47] (mis=4)
411-463 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 12 umis using 2240 reads
cdr3 = CSVEAPSGTSGNNEQYF at 332, score = 7 + 8
umis assigned: [2, 4, 19, 106, 140, 175, 206, 213, 240, 249] and 4 others
of which 14 are surviving nonsolos
reads assigned: 36026
start codons at 11, 146, 149, 225, 302
confident = true

TIG 2[bases=487]
0-66 ==> 0-66 on |451|TRAV1-1|5'UTR| [len=66] (mis=0)
66-392 ==> 0-326 on |452|TRAV1-1|L-REGION+V-REGION| [len=326] (mis=2)
392-445 ==> 8-61 on |450|TRAJ9|J-REGION| [len=61] (mis=0)
445-487 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 3 umis using 852 reads
cdr3 = CAVGDGGFKTIF at 378, score = 7 + 8
umis assigned: [48, 171, 292, 345, 387]
of which 4 are surviving nonsolos
reads assigned: 14026
start codons at 33, 49, 66, 88, 96, 102, 205, 229, 232, 262, 271, 325, 354, 391
confident = true
now this is a cell
paired!

GAGCTCCAGATGAAAGACTCTGCCTCTTACTTCTGCGCTGTGGGGGATGGAGGCTTCAAAACTATCTTTGGAGCAGGAACAAGACTATTTGTTAAAGCAA <==> GAAGACAGCAGCATATATCTCTGCAGCGTTGAAGCCCCCTCCGGGACTAGCGGGAACAACGAGCAGTACTTCGGGCCGGGCACCAGGCTCACGGTCACAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.749 = ACGGCCACAGCTCGAC-1

using 43 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[43]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.751 = ACGGCCACAGGGTTAG-1

using 69 reads

====================================================================================

graph has 98 edges initially, 2 edges after simplification

total ucounts = 15
nonsolo ucounts = 9[2^3, 3, 4^2, 8, 17, 21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.754 = ACGGCCACAGTAGAGC-1

using 131 reads

====================================================================================

graph has 100 edges initially, 4 edges after simplification

total ucounts = 10
nonsolo ucounts = 6[2^2, 3, 16, 26, 78]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.757 = ACGGCCACATAGAAAC-1

using 138 reads

====================================================================================

graph has 114 edges initially, 4 edges after simplification

total ucounts = 15
nonsolo ucounts = 11[2^3, 3^2, 4, 6^2, 22, 25, 59]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.764 = ACGGCCACATTACCTT-1

using 115 reads

====================================================================================

graph has 68 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[115]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.773 = ACGGCCAGTCAAACTC-1

using 62 reads

====================================================================================

graph has 82 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[62]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.776 = ACGGCCAGTCACCTAA-1

using 185 reads

====================================================================================

graph has 114 edges initially, 4 edges after simplification

total ucounts = 12
nonsolo ucounts = 7[2, 3^3, 6, 80, 83]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.779 = ACGGCCAGTCATCCCT-1

using 239 reads

====================================================================================

graph has 206 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[239]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.786 = ACGGCCAGTGACCAAG-1

using 24 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.787 = ACGGCCAGTGACGCCT-1

using 26 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.788 = ACGGCCAGTGATGTGG-1

using 25 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.794 = ACGGCCAGTGTAATGA-1

using 493 reads

====================================================================================

graph has 366 edges initially, 14 edges after simplification

total ucounts = 19
nonsolo ucounts = 17[2, 3, 4, 9, 11, 14, 21, 22, 24, 25, 38, 39, 42, 44, 57, 61, 75]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.795 = ACGGCCAGTGTGACGA-1

using 68 reads

====================================================================================

graph has 98 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 7[2^2, 3, 4, 8, 23, 24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.800 = ACGGCCAGTTCGTCTC-1

using 52 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[3^2, 45]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.804 = ACGGCCATCAAGGTAA-1

using 1825 reads

====================================================================================

graph has 1774 edges initially, 66 edges after simplification

total ucounts = 229
nonsolo ucounts = 171[2^28, 3^13, 4^22, 5^10, 6^7, 7^14, 8^3, 9^3, 10^7, 11^10, 12^4, 13^7, 14^4, 15^2, 16^6, 17^5, 18^2, 19^2, 20^3, 21^2, 22^2, 23, 25^2, 26^2, 27, 28, 29^2, 35, 36, 51, 52, 56, 69]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.806 = ACGGCCATCACAAACC-1

using 70 reads

====================================================================================

graph has 126 edges initially, 2 edges after simplification

total ucounts = 12
nonsolo ucounts = 5[2^2, 3, 7, 49]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.809 = ACGGCCATCACGGTTA-1

using 33 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.812 = ACGGCCATCATAGCAC-1

using 68 reads

====================================================================================

graph has 88 edges initially, 2 edges after simplification

total ucounts = 15
nonsolo ucounts = 7[2, 3, 4^2, 6, 16, 25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.814 = ACGGCCATCATGGTCA-1

using 52 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[12, 40]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.817 = ACGGCCATCCAGTATG-1

using 45 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[45]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.820 = ACGGCCATCCCTCTTT-1

using 89 reads

====================================================================================

graph has 52 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[88]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.822 = ACGGCCATCCGTCATC-1

using 301 reads

====================================================================================

graph has 282 edges initially, 14 edges after simplification

total ucounts = 24
nonsolo ucounts = 21[2^5, 3^3, 4, 5^3, 7, 10, 22, 28, 29, 34, 38, 40, 52]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.826 = ACGGCCATCCTCATTA-1

using 161 reads

====================================================================================

graph has 138 edges initially, 4 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[39, 120]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.829 = ACGGCCATCCTTTCTC-1

using 26 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.830 = ACGGCCATCGAGAACG-1

using 47 reads

====================================================================================

graph has 48 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[2, 4, 13, 26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.831 = ACGGCCATCGCGATCG-1

using 18 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.835 = ACGGCCATCGGTCTAA-1

using 7146 reads

====================================================================================

graph has 6894 edges initially, 162 edges after simplification

total ucounts = 1110
nonsolo ucounts = 767[2^155, 3^123, 4^87, 5^54, 6^45, 7^41, 8^31, 9^38, 10^22, 11^18, 12^16, 13^25, 14^11, 15^6, 16^7, 17^9, 18^2, 19^9, 20^3, 21^6, 22^5, 23^2, 24^5, 25^4, 26^3, 27^2, 28, 29^2, 31^3, 32, 33^4, 35^2, 36^3, 40, 41, 42, 48^2, 50, 52^3, 55, 56, 58, 65, 67, 73, 79^2, 82, 85, 96, 101, 172]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.846 = ACGGCCATCTGCCAGG-1

using 80 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[80]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.848 = ACGGCCATCTGTTTGT-1

using 58 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 5[2, 3, 16, 17, 19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.849 = ACGGCCATCTTCAACT-1

using 94 reads

====================================================================================

graph has 127 edges initially, 2 edges after simplification

total ucounts = 19
nonsolo ucounts = 12[2^2, 3, 4, 5, 6^3, 8, 12^2, 21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.853 = ACGGGCTAGAAACGAG-1

using 27 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.855 = ACGGGCTAGAACTGTA-1

using 20 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.857 = ACGGGCTAGAATTCCC-1

using 30 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.859 = ACGGGCTAGACTAAGT-1

using 29 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.861 = ACGGGCTAGAGAACAG-1

using 2292 reads

====================================================================================

graph has 1022 edges initially, 16 edges after simplification

total ucounts = 158
nonsolo ucounts = 132[2^10, 3^12, 4^10, 5^4, 6, 7^7, 8^4, 9^2, 10^6, 11^4, 12, 13^4, 14^5, 15, 16^3, 17^6, 18^5, 20^3, 21^5, 22^3, 23, 24^2, 25^2, 26^2, 27, 28^4, 29^3, 30^4, 32, 34^2, 35, 36, 37, 43, 44, 45, 46, 47, 53, 54, 57, 65^2, 75]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.862 = ACGGGCTAGAGACTTA-1

using 112 reads

====================================================================================

graph has 78 edges initially, 4 edges after simplification

total ucounts = 7
nonsolo ucounts = 5[3^2, 5, 44, 55]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.863 = ACGGGCTAGATCACGG-1

using 44 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[43]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.865 = ACGGGCTAGCACACAG-1

using 45 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[45]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.872 = ACGGGCTAGCGGATCA-1

using 2017 reads

====================================================================================

graph has 750 edges initially, 4 edges after simplification

total ucounts = 5
nonsolo ucounts = 4[2, 4, 38, 1972]
surviving nonsolo ucounts = 1[1972]
ids = [4]

====================================================================================

REJECT CONTIGS

TIG 1[bases=439]
0-31 ==> 36-67 on |614|TRBV29-1|5'UTR| [len=67] (mis=0)
0-31 ==> 5969-6000 on segment before TRBV29OR9-2 exon 1 [len=6000] (mis=0)
31-65 ==> 0-34 on |615|TRBV29-1|L-REGION+V-REGION| [len=335] (mis=0)
63-328 ==> 64-329 on |615|TRBV29-1|L-REGION+V-REGION| [len=335] (mis=0) [SHIFT!]
337-387 ==> 0-50 on |547|TRBJ2-4|J-REGION| [len=50] (mis=5)
387-439 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
cdr3 = CSVTTGPVNIQYF at 320, score = 7 + 8
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 1948
start codons at 31, 134, 137, 213, 290
confident = false
not full
frameshifted full length stopped transcript of length 439
VJ delta = 48
delta too large
not full
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.887 = ACGGGCTAGGCTCAGA-1

using 19 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[2, 17]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.892 = ACGGGCTAGTACACCT-1

using 91 reads

====================================================================================

graph has 96 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 9[2^2, 4^2, 6, 13, 14, 17, 27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.893 = ACGGGCTAGTACGCGA-1

using 7120 reads

====================================================================================

graph has 5075 edges initially, 116 edges after simplification

total ucounts = 644
nonsolo ucounts = 433[2^85, 3^42, 4^42, 5^46, 6^30, 7^21, 8^21, 9^16, 10^18, 11^10, 12^13, 13^11, 14^8, 15^4, 16^7, 17^5, 18^2, 19^7, 20^4, 21^2, 22^3, 23, 24^2, 25^5, 26^2, 27^5, 28, 29, 30, 31^2, 32, 34, 35, 37, 39, 41^2, 43, 44, 46, 47, 52, 53, 56, 60, 3069]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.898 = ACGGGCTAGTGTACCT-1

using 30 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.904 = ACGGGCTCAAACCTAC-1

using 42 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 5[2, 4, 5, 13, 18]
surviving nonsolo ucounts = 1[18]
ids = [2]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.905 = ACGGGCTCAAACGTGG-1

using 58 reads

====================================================================================

graph has 92 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 6[2, 3, 5, 9, 12, 25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.911 = ACGGGCTCAAGAAGAG-1

using 40 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[40]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.912 = ACGGGCTCAAGCCCAC-1

using 33 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 2[2, 26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.914 = ACGGGCTCAAGGTGTG-1

using 91 reads

====================================================================================

graph has 98 edges initially, 2 edges after simplification

total ucounts = 13
nonsolo ucounts = 7[2^3, 4, 14, 15, 46]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.919 = ACGGGCTCAATGTTGC-1

using 619 reads

====================================================================================

graph has 234 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 5[2, 3, 5, 14, 592]
surviving nonsolo ucounts = 1[592]
ids = [7]

====================================================================================

UMI info for barcode ACGGGCTCAATGTTGC-1 contig 1 = GGGGATCCTG...
umi TCCACTTACC = 589 reads: +403 validated

GOOD CONTIGS

TIG 1[bases=478]
23-370 ==> 0-347 on |688|TRBV7-9|L-REGION+V-REGION| [len=347] (mis=3)
379-426 ==> 0-47 on |550|TRBJ2-7|J-REGION| [len=47] (mis=2)
426-478 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 1 umis using 33 reads
cdr3 = CASSPTGFGAYEQYF at 353, score = 7 + 8
umis assigned: [7]
of which 1 are surviving nonsolos
reads assigned: 580
start codons at 23, 47, 132, 231, 344
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.924 = ACGGGCTCACATAACC-1

using 80000 reads

====================================================================================

graph has 21440 edges initially, 176 edges after simplification

total ucounts = 1311
nonsolo ucounts = 828[2^161, 3^104, 4^76, 5^77, 6^46, 7^34, 8^26, 9^35, 10^14, 11^32, 12^17, 13^18, 14^12, 15^8, 16^9, 17^8, 18^15, 19^5, 20^5, 21^3, 22^3, 23^5, 24^4, 25^6, 26^3, 27^5, 28^3, 29^4, 30^2, 31^3, 32^2, 33, 34^3, 35^3, 36^2, 37^3, 38^2, 39^3, 40^3, 41, 43, 44, 45^2, 46, 47^2, 49, 53, 54^3, 58, 60, 61, 62, 65, 68, 69, 90, 104, 115, 121, 153, 563, 645, 689, 716, 728, 742, 751, 793, 864, 932, 937, 941, 1058, 1075, 1095, 1096, 1116, 1155, 1171, 1175, 1244, 1278, 1317, 1318, 1505, 1678, 1805, 2128, 2347, 2350, 2638, 2922, 3176, 3751, 4553, 5595, 6672, 7171]
surviving nonsolo ucounts = 38[563, 645, 689, 716, 728, 742, 751, 793, 864, 932, 937, 941, 1058, 1075, 1095, 1096, 1116, 1155, 1171, 1175, 1244, 1278, 1317, 1318, 1505, 1678, 1805, 2128, 2347, 2350, 2638, 2922, 3176, 3751, 4553, 5595, 6672, 7171]
ids = [399, 1174, 220, 350, 735, 827, 832, 115, 1181, 319, ...]

====================================================================================

UMI info for barcode ACGGGCTCACATAACC-1 contig 1 = GTGATTTCTT...
umi AAGAGACTGC = 2141 reads: +397 validated
umi CCTTAATGTC = 1514 reads: +397 validated
umi TACGCTAGGA = 3242 reads: +397 validated
umi TAGCGGTTCA = 1180 reads: +397 validated
umi TCAGAGGATC = 1808 reads: +397 validated
umi TCTTTACCAC = 2659 reads: +397 validated

UMI info for barcode ACGGGCTCACATAACC-1 contig 2 = TTCCTCTGCT...
umi ACCTAGTCTG = 931 reads: +382 validated
umi ACTTTAACCT = 1043 reads: +382 validated
umi AGGCACCCAA = 692 reads: +382 validated
umi ATGTCAAGAG = 1093 reads: +382 validated
umi ATTCACTTAA = 939 reads: +382 validated
umi CAACGGATCG = 708 reads: +382 validated
umi CACCATGCTG = 1312 reads: +382 validated
umi CAGTGGTCCT = 507 reads: +356 -6 +20 non-validated
umi CCCGTAGCTT = 1089 reads: +382 validated
umi CCCTCAGCGA = 2356 reads: +382 validated
umi CGGACTTGCT = 1242 reads: +382 validated
umi GAACACACCC = 1281 reads: +382 validated
umi GCCCTTAACA = 943 reads: +382 validated
umi GCTGACCCAA = 619 reads: +382 validated
umi GTATCTTCTA = 680 reads: +382 validated
umi GTCATGGGAA = 752 reads: +382 validated
umi TCACACCACC = 1114 reads: +382 validated
umi TCATAATTCC = 1073 reads: +382 validated
umi TCCACCCTTG = 1155 reads: +382 validated
umi TGACTGCTCA = 1332 reads: +382 validated
umi TGGCGTAGGA = 1166 reads: +382 validated
umi TGGGCCTCAT = 588 reads: +382 validated
umi TGTAACGTAC = 864 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=491]
0-52 ==> 4-56 on |529|TRAV9-2|5'UTR| [len=56] (mis=0)
52-390 ==> 0-338 on |530|TRAV9-2|L-REGION+V-REGION| [len=338] (mis=1)
386-449 ==> 0-63 on |408|TRAJ23|J-REGION| [len=63] (mis=5)
449-491 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 6 umis using 740 reads
cdr3 = CALSDHNQGGKLIF at 376, score = 9 + 9
umis assigned: [48, 471, 943, 964, 999, 1102]
of which 6 are surviving nonsolos
reads assigned: 12242
start codons at 11, 52, 127, 215, 269
confident = true

TIG 2[bases=546]
0-112 ==> 0-112 on |618|TRBV30|5'UTR| [len=112] (mis=0)
112-447 ==> 0-335 on |619|TRBV30|L-REGION+V-REGION| [len=335] (mis=4)
439-451 ==> 0-12 on |535|TRBD1|D-REGION| [len=12] (mis=1)
447-494 ==> 0-47 on |550|TRBJ2-7|J-REGION| [len=47] (mis=3)
494-546 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 22 umis using 1009 reads
cdr3 = CAGTGVYEQYF at 433, score = 7 + 8
umis assigned: [119, 180, 220, 310, 319, 350, 366, 399, 428, 432] and 13 others
of which 23 are surviving nonsolos
reads assigned: 23039
start codons at 109, 112, 180
confident = true

REJECT CONTIGS

TIG 1[bases=370]
3-115 ==> 2466-2578 on segment before TRBJ2-1 exon 1 [len=3226] (mis=0)
166-268 ==> 0-102 on segment before TRBJ2-4 exon 1 [len=102] (mis=0)
268-318 ==> 0-50 on |547|TRBJ2-4|J-REGION| [len=50] (mis=0)
318-370 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
umis assigned: [115, 116, 898, 1123, 1154, 1239]
of which 6 are surviving nonsolos
reads assigned: 21703
start codons at 87
confident = false
did not find CDR3

TIG 2[bases=407]
3-204 ==> 5135-5336 on segment before TRAV8-3 exon 1 [len=5336] (mis=0)
204-365 ==> 0-161 on |522|TRAV8-3|L-REGION+V-REGION| [len=341] (mis=0)
365-407 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
umis assigned: [165, 514, 1055]
of which 3 are surviving nonsolos
reads assigned: 13195
start codons at 204, 326, 340
confident = false
did not find CDR3
now this is a cell
paired!

CAAGTGTCAGACTCAGCGGTGTACTTCTGTGCTCTGAGTGATCATAACCAGGGAGGAAAGCTTATCTTCGGACAGGGAACGGAGTTATCTGTGAAACCCA <==> TCTAAGAAGCTCCTTCTCAGTGACTCTGGCTTCTATCTCTGTGCCGGGACAGGGGTCTACGAGCAGTACTTCGGGCCGGGCACCAGGCTCACGGTCACAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.938 = ACGGGCTCACGGTGTC-1

using 9936 reads

====================================================================================

graph has 5578 edges initially, 138 edges after simplification

total ucounts = 397
nonsolo ucounts = 322[2^24, 3^19, 4^11, 5^14, 6^7, 7^8, 8^13, 9^6, 10^5, 11^7, 12^8, 13^11, 14^9, 15^4, 17^10, 18^7, 19^2, 20^5, 21^2, 22^4, 23^3, 24^4, 25^5, 26^2, 27^5, 28^4, 29^4, 30^7, 31^3, 32^6, 34^3, 35^3, 36, 37^3, 38^3, 39^3, 40^4, 41^3, 42, 43^3, 44, 45^3, 46^5, 47^2, 48, 49^2, 50^2, 51^3, 52^2, 53, 54^2, 55^3, 56^2, 58^2, 59, 61^3, 62, 63^3, 65^4, 66^2, 69^3, 70, 71, 72, 73^2, 74, 75^2, 76^2, 77^2, 79, 80, 81, 83, 91^2, 92^2, 93, 96, 98^2, 99, 101, 106, 1110]
surviving nonsolo ucounts = 1[1110]
ids = [248]

====================================================================================

UMI info for barcode ACGGGCTCACGGTGTC-1 contig 1 = AGCCTGTCTT...
umi GCGGCATCTC = 1133 reads: +400 validated

GOOD CONTIGS

TIG 1[bases=606]
0-154 ==> 54-208 on |589|TRBV19|5'UTR| [len=208] (mis=0)
154-498 ==> 0-344 on |590|TRBV19|L-REGION+V-REGION| [len=344] (mis=2)
506-554 ==> 0-48 on |536|TRBJ1-1|J-REGION| [len=48] (mis=2)
554-606 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 1 umis using 32 reads
cdr3 = CASSMAQGLGTEAFF at 481, score = 6 + 8
umis assigned: [248]
of which 1 are surviving nonsolos
reads assigned: 1096
start codons at 154, 212, 263, 299, 304, 368, 493
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.949 = ACGGGCTCAGGACCCT-1

using 91 reads

====================================================================================

graph has 62 edges initially, 8 edges after simplification

total ucounts = 6
nonsolo ucounts = 5[3^2, 5, 38, 41]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.950 = ACGGGCTCAGGCGATA-1

using 69 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[69]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.952 = ACGGGCTCAGGTCCAC-1

using 70 reads

====================================================================================

graph has 72 edges initially, 4 edges after simplification

total ucounts = 8
nonsolo ucounts = 6[2^2, 3, 8, 22, 31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.954 = ACGGGCTCAGGTTTCA-1

using 39 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 5[5^2, 7, 8, 10]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.955 = ACGGGCTCAGTAACGG-1

using 88 reads

====================================================================================

graph has 76 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 5[2^2, 15, 16, 51]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.959 = ACGGGCTCAGTTCATG-1

using 33 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.967 = ACGGGCTCATGCAATC-1

using 73 reads

====================================================================================

graph has 72 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 3[10, 21, 42]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.968 = ACGGGCTCATGCCTTC-1

using 25 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.971 = ACGGGCTCATGTCCTC-1

using 66 reads

====================================================================================

graph has 112 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[66]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.972 = ACGGGCTCATGTTCCC-1

using 198 reads

====================================================================================

graph has 92 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[8, 190]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.976 = ACGGGCTCATTGAGCT-1

using 10003 reads

====================================================================================

graph has 8747 edges initially, 214 edges after simplification

total ucounts = 1097
nonsolo ucounts = 789[2^122, 3^100, 4^84, 5^45, 6^39, 7^36, 8^27, 9^27, 10^33, 11^20, 12^23, 13^23, 14^24, 15^17, 16^17, 17^10, 18^14, 19^9, 20^8, 21^10, 22^10, 23^6, 24^8, 25^6, 26^3, 27^3, 28^6, 29, 30^2, 32^2, 33, 35^3, 36^4, 38, 39^2, 41^3, 42^2, 43^3, 44^2, 45, 46^2, 47^5, 48, 49, 53, 54, 58^2, 61^2, 62^2, 63, 66, 68^2, 71, 74, 78, 82, 87, 92, 110, 127, 141, 147, 480]
surviving nonsolo ucounts = 2[141, 480]
ids = [428, 119]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.979 = ACGGGCTCATTTGCCC-1

using 1154 reads

====================================================================================

graph has 937 edges initially, 20 edges after simplification

total ucounts = 180
nonsolo ucounts = 107[2^25, 3^13, 4^8, 5^6, 6^6, 7^3, 8^5, 9^8, 10^3, 11^5, 12, 13^2, 14^3, 15, 16, 17, 19, 20, 21, 22, 27, 28, 29, 32, 35^2, 36, 39, 49, 50^2, 56]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.982 = ACGGGCTGTAAGTGGC-1

using 8313 reads

====================================================================================

graph has 8918 edges initially, 228 edges after simplification

total ucounts = 1065
nonsolo ucounts = 792[2^141, 3^98, 4^66, 5^52, 6^45, 7^42, 8^38, 9^29, 10^30, 11^25, 12^25, 13^23, 14^20, 15^26, 16^13, 17^13, 18^6, 19^4, 20^7, 21^8, 22^5, 23^4, 24^7, 25^5, 26^7, 27, 28^4, 29^3, 30^4, 31^3, 33^4, 34^4, 35^2, 36^2, 37^3, 41, 42^2, 43^2, 47, 48^4, 49, 55, 56, 60, 70, 73^2, 77, 80, 85, 88, 89, 104]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.983 = ACGGGCTGTAAGTGTA-1

using 22 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.984 = ACGGGCTGTAAGTTCC-1

using 32806 reads

====================================================================================

graph has 12314 edges initially, 124 edges after simplification

total ucounts = 673
nonsolo ucounts = 471[2^60, 3^57, 4^45, 5^36, 6^33, 7^32, 8^19, 9^23, 10^16, 11^12, 12^15, 13^17, 14^10, 15^7, 16^6, 17^9, 18^6, 19^6, 20^2, 21^3, 23^3, 24^2, 25^5, 26^2, 27, 28^2, 29, 30, 31^5, 32^2, 33, 34, 36, 37, 39, 41^2, 42, 46, 48, 51, 53, 144, 170, 175, 379, 410, 427, 496, 846, 1061, 1301, 1347, 1479, 1628, 1648, 1837, 1914, 2031, 2375, 2653, 2840, 3343]
surviving nonsolo ucounts = 20[170, 175, 379, 410, 427, 496, 846, 1061, 1301, 1347, 1479, 1628, 1648, 1837, 1914, 2031, 2375, 2653, 2840, 3343]
ids = [461, 306, 81, 279, 616, 322, 58, 460, 375, 400, ...]

====================================================================================

UMI info for barcode ACGGGCTGTAAGTTCC-1 contig 1 = TTTATGGGGA...
umi AACGTCATCG = 1637 reads: -10 +2 -2XX +446 invalidated
umi ACACATCTAG = 855 reads: +460 validated
umi CGTGTTACTA = 406 reads: +460 validated
umi GCTCGTTAGA = 1351 reads: +192 -3XX +265 invalidated
umi GTATTGCTTT = 1657 reads: +460 validated
umi TTGACTCTGC = 2833 reads: -84X +2 -2XX +1 -2XX +3 -1XX +1 -2XX +1 -10XX +351 invalidated

UMI info for barcode ACGGGCTGTAAGTTCC-1 contig 2 = AGACCAGAAT...
umi AACGGCGGCC = 1935 reads: +397 validated
umi ACGCCAGGTA = 379 reads: +397 validated
umi CCTATGGTGT = 2377 reads: +397 validated
umi CTGCGACACA = 1467 reads: +397 validated
umi GCCTCGTTCG = 2040 reads: +397 validated
umi GGTACCACGA = 1353 reads: +397 validated
umi GTGCACAACC = 1673 reads: -362X +1 -5XX +2 -2XX +2 -15XX +1 -5XX +2 invalidated
umi GTGCTTTCGT = 1858 reads: +397 validated
umi TGACACATAC = 2659 reads: +397 validated
umi TTAAGATGGG = 374 reads: +397 validated

GOOD CONTIGS

TIG 1[bases=518]
16-413 ==> 0-397 on |516|TRAV6|L-REGION+V-REGION| [len=397] (mis=5)
413-476 ==> 0-63 on |407|TRAJ22|J-REGION| [len=63] (mis=4)
476-518 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 6 umis using 556 reads
cdr3 = CALVGPSGSARQLTF at 400, score = 8 + 9
umis assigned: [21, 58, 279, 375, 427, 648]
of which 6 are surviving nonsolos
reads assigned: 8486
start codons at 3, 16, 73, 290
confident = true

TIG 2[bases=496]
0-47 ==> 0-47 on |616|TRBV3-1|5'UTR| [len=47] (mis=0)
47-391 ==> 0-344 on |617|TRBV3-1|L-REGION+V-REGION| [len=344] (mis=4)
394-444 ==> 0-50 on |538|TRBJ1-3|J-REGION| [len=50] (mis=4)
444-496 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 9 umis using 1147 reads
cdr3 = CASSSGTVRNTIYF at 374, score = 9 + 7
umis assigned: [20, 81, 240, 305, 361, 400, 439, 447, 573, 616]
of which 10 are surviving nonsolos
reads assigned: 15834
start codons at 47, 146, 169, 189, 197, 239, 273
confident = true

REJECT CONTIGS

TIG 1[bases=623]
0-176 ==> 0-176 on |496|TRAV3|5'UTR| [len=176] (mis=1)
176-519 ==> 0-343 on |497|TRAV3|L-REGION+V-REGION| [len=343] (mis=2)
521-581 ==> 0-60 on |449|TRAJ8|J-REGION| [len=60] (mis=3)
581-623 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
umis assigned: [306, 460]
of which 2 are surviving nonsolos
reads assigned: 1177
start codons at 176, 197, 206, 298, 339, 414
confident = false
frameshifted full length stopped transcript of length 623
did not find CDR3

TIG 2[bases=480]
0-31 ==> 6-37 on |606|TRBV25-1|5'UTR| [len=37] (mis=0)
31-375 ==> 0-344 on |607|TRBV25-1|L-REGION+V-REGION| [len=344] (mis=3)
378-428 ==> 0-50 on |547|TRBJ2-4|J-REGION| [len=50] (mis=1)
428-480 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
umis assigned: [322, 461]
of which 2 are surviving nonsolos
reads assigned: 625
start codons at 31, 55, 85, 153, 166, 173, 181, 208, 236
confident = false
did not find CDR3
now this is a cell
paired!

CCTGCAGACTCAGCTACCTACCTCTGTGCTCTAGTAGGCCCATCTGGTTCTGCAAGGCAACTGACCTTTGGATCTGGGACACAATTGACTGTTTTACCTG <==> CTGGAGCTTGGTGACTCTGCTGTGTATTTCTGTGCCAGCAGCTCGGGGACAGTTCGAAACACCATATATTTTGGAGAGGGAAGTTGGCTCACTGTTGTAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.985 = ACGGGCTGTACAGTGG-1

using 25 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.990 = ACGGGCTGTAGCCTAT-1

using 107 reads

====================================================================================

graph has 140 edges initially, 2 edges after simplification

total ucounts = 28
nonsolo ucounts = 18[2^7, 3^2, 4^4, 5, 8, 13, 17, 18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.994 = ACGGGCTGTATTCGTG-1

using 869 reads

====================================================================================

graph has 797 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[868]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.996 = ACGGGCTGTCAATGTC-1

using 89 reads

====================================================================================

graph has 116 edges initially, 4 edges after simplification

total ucounts = 17
nonsolo ucounts = 12[2^3, 3, 4, 5^2, 6, 8, 11, 17, 19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.1002 = ACGGGCTGTCCCGACA-1

using 85 reads

====================================================================================

graph has 62 edges initially, 4 edges after simplification

total ucounts = 5
nonsolo ucounts = 4[8, 9, 19, 48]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.1008 = ACGGGCTGTCGTTGTA-1

using 295 reads

====================================================================================

graph has 350 edges initially, 6 edges after simplification

total ucounts = 71
nonsolo ucounts = 37[2^7, 3^4, 4^6, 5^2, 6^3, 7^5, 8, 9, 11^2, 12, 13, 16, 19^2, 30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.1009 = ACGGGCTGTCTCATCC-1

using 18 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.1010 = ACGGGCTGTCTGATCA-1

using 64 reads

====================================================================================

graph has 29 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[64]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.1016 = ACGGGCTGTGAGGCTA-1

using 61 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[61]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.1019 = ACGGGCTGTGATGTCT-1

using 84 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[84]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.1033 = ACGGGCTGTTCACCTC-1

using 59 reads

====================================================================================

graph has 66 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 3[5, 26, 28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.1038 = ACGGGCTGTTGGGACA-1

using 30 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.1041 = ACGGGCTTCAAACCAC-1

using 30 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.1047 = ACGGGCTTCACAAACC-1

using 27 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.1053 = ACGGGCTTCACTCCTG-1

using 186 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[186]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.1058 = ACGGGCTTCAGATAAG-1

using 20 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.1060 = ACGGGCTTCAGGCGAA-1

using 35 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 4[2^2, 10, 20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.1061 = ACGGGCTTCAGTACGT-1

using 118 reads

====================================================================================

graph has 96 edges initially, 6 edges after simplification

total ucounts = 13
nonsolo ucounts = 7[2^2, 4, 7, 13, 29, 55]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.1066 = ACGGGCTTCATCGCTC-1

using 45 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[45]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.1070 = ACGGGCTTCCAGAAGG-1

using 571 reads

====================================================================================

graph has 390 edges initially, 20 edges after simplification

total ucounts = 38
nonsolo ucounts = 25[2^3, 3^2, 4, 5, 7, 8, 12, 14, 15, 17, 18, 21, 24^2, 28, 31, 35, 40, 44, 58^2, 83]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.1071 = ACGGGCTTCCAGATCA-1

using 76 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[76]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.1075 = ACGGGCTTCCCTTGCA-1

using 78 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[78]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.1078 = ACGGGCTTCCGTCAAA-1

using 66 reads

====================================================================================

graph has 56 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 3[7^2, 44]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.1080 = ACGGGCTTCCTGCAGG-1

using 15 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.1081 = ACGGGCTTCCTTTCGG-1

using 337 reads

====================================================================================

graph has 282 edges initially, 12 edges after simplification

total ucounts = 27
nonsolo ucounts = 17[2^3, 3, 4, 5, 7^2, 10, 11, 16, 19, 20, 39, 55, 58, 67]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.1082 = ACGGGCTTCGAATCCA-1

using 82 reads

====================================================================================

graph has 82 edges initially, 2 edges after simplification

total ucounts = 18
nonsolo ucounts = 12[2^3, 3^3, 4, 5, 10^2, 13, 19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.1083 = ACGGGCTTCGACGGAA-1

using 19 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[2, 15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.1084 = ACGGGCTTCGAGAACG-1

using 73 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[73]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.1088 = ACGGGCTTCGGAAATA-1

using 83 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[83]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.1091 = ACGGGCTTCGGCGGTT-1

using 94 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[94]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.1092 = ACGGGCTTCGGTCCGA-1

using 13 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[13]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.1094 = ACGGGCTTCGTAGGTT-1

using 31 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.1096 = ACGGGCTTCGTCTGAA-1

using 37 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.1099 = ACGGGCTTCTAACTTC-1

using 69 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[68]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.1111 = ACGGGCTTCTGGGCCA-1

using 49 reads

====================================================================================

graph has 48 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 5[2^2, 3^2, 37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.1112 = ACGGGCTTCTGGTATG-1

using 18 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.1114 = ACGGGCTTCTGTCTAT-1

using 23 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.1116 = ACGGGCTTCTTACCTA-1

using 29 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.1128 = ACGGGTCAGAGTACCG-1

using 13 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[13]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.1133 = ACGGGTCAGATCTGCT-1

using 1499 reads

====================================================================================

graph has 572 edges initially, 6 edges after simplification

total ucounts = 18
nonsolo ucounts = 8[3, 4, 6, 7, 9, 35, 71, 1354]
surviving nonsolo ucounts = 1[1354]
ids = [10]

====================================================================================

UMI info for barcode ACGGGTCAGATCTGCT-1 contig 1 = AAAGCAGATT...
umi TCCAGGGTCA = 1356 reads: +397 validated

GOOD CONTIGS

TIG 1[bases=544]
0-105 ==> 0-105 on |459|TRAV12-2|5'UTR| [len=105] (mis=0)
105-445 ==> 0-340 on |460|TRAV12-2|L-REGION+V-REGION| [len=340] (mis=0)
448-502 ==> 7-61 on |450|TRAJ9|J-REGION| [len=61] (mis=0)
502-544 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 1 umis using 65 reads
cdr3 = CAVNMPGGFKTIF at 432, score = 9 + 8
umis assigned: [10]
of which 1 are surviving nonsolos
reads assigned: 1335
start codons at 16, 102, 105, 309, 325, 340, 379, 444
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.1140 = ACGGGTCAGCCGCCTA-1

using 7433 reads

====================================================================================

graph has 5150 edges initially, 66 edges after simplification

total ucounts = 771
nonsolo ucounts = 476[2^99, 3^79, 4^63, 5^43, 6^30, 7^24, 8^19, 9^18, 10^10, 11^15, 12^8, 13^9, 14^7, 15^3, 16^3, 17, 18, 19^5, 20^2, 21, 22, 23^4, 24, 26^2, 27^2, 28, 29^2, 30, 32, 33, 34^2, 36^5, 37, 38, 39, 45, 50, 53, 57^2, 62, 66, 70, 72, 3395]
surviving nonsolo ucounts = 1[3395]
ids = [702]

====================================================================================

REJECT CONTIGS

TIG 1[bases=393]
36-140 ==> 0-104 on segment before TRBJ2-3 exon 1 [len=104] (mis=0)
140-189 ==> 0-49 on |546|TRBJ2-3|J-REGION| [len=49] (mis=0)
189-291 ==> 0-102 on segment before TRBJ2-4 exon 1 [len=102] (mis=0)
341-393 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
umis assigned: [702]
of which 1 are surviving nonsolos
reads assigned: 3343
start codons at 50
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.1141 = ACGGGTCAGCGAAGGG-1

using 39 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[39]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.1145 = ACGGGTCAGCGCCTTG-1

using 4002 reads

====================================================================================

graph has 1150 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 5[2^2, 3, 6, 3983]
surviving nonsolo ucounts = 1[3983]
ids = [7]

====================================================================================

REJECT CONTIGS

TIG 1[bases=417]
4-313 ==> 29-338 on |530|TRAV9-2|L-REGION+V-REGION| [len=338] (mis=1)
317-375 ==> 8-66 on |413|TRAJ28|J-REGION| [len=66] (mis=0)
375-417 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
cdr3 = CALSDLGAGSYQLTF at 299, score = 9 + 10
umis assigned: [7]
of which 1 are surviving nonsolos
reads assigned: 3931
start codons at 50, 138, 192
confident = false
VJ delta = 29
not full
not full
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.1146 = ACGGGTCAGCTAAACA-1

using 46 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[45]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.1148 = ACGGGTCAGCTAGCCC-1

using 61934 reads

====================================================================================

graph has 12477 edges initially, 153 edges after simplification

total ucounts = 934
nonsolo ucounts = 558[2^131, 3^91, 4^46, 5^43, 6^32, 7^24, 8^19, 9^14, 10^9, 11^12, 12^8, 13^10, 14^8, 15^7, 16^6, 17^3, 18^3, 19^13, 20^4, 21^2, 22^6, 23^4, 24^3, 25^4, 26, 27, 28, 29^2, 30^3, 31^3, 32^3, 33, 34, 35^2, 36^2, 37, 38, 39, 41, 42, 43, 44, 45, 46, 47, 48^2, 51, 55, 56, 58^2, 60, 61, 65, 67, 74, 78, 82, 94, 102, 256, 427, 4396, 6655, 6884, 6989, 7060, 7306, 8138^2]
surviving nonsolo ucounts = 8[4396, 6655, 6884, 6989, 7060, 7306, 8138^2]
ids = [905, 432, 625, 105, 517, 212, 348, 608]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.1160 = ACGGGTCAGGCATGTG-1

using 44 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[44]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.1164 = ACGGGTCAGGCGTACA-1

using 12176 reads

====================================================================================

graph has 5336 edges initially, 60 edges after simplification

total ucounts = 514
nonsolo ucounts = 341[2^75, 3^68, 4^46, 5^20, 6^20, 7^11, 8^12, 9^7, 10^2, 11^11, 12^6, 13^3, 14^5, 15^8, 16, 17^5, 18^2, 19^3, 20, 21^2, 22^2, 23, 24, 25^3, 26^2, 29^2, 31, 32^2, 33, 36, 37, 38^2, 40, 41, 47, 49, 55, 73, 108, 282, 1157, 1293, 1479, 1578, 1596, 1960]
surviving nonsolo ucounts = 7[282, 1157, 1293, 1479, 1578, 1596, 1960]
ids = [222, 247, 119, 479, 468, 279, 41]

====================================================================================

UMI info for barcode ACGGGTCAGGCGTACA-1 contig 1 = GCCAGGTTCA...
umi ATGCTTAGCA = 1290 reads: +412 validated
umi CTCCACCCAC = 1202 reads: +27 -6XX +1 -2XX +1 -5XX +1 -47XX +4 -3XX +1 -9XX +1 -2XX +1 -3XX +298 invalidated
umi GAAATATGCT = 1599 reads: +412 validated
umi TTACTCCGCA = 1577 reads: +412 validated

UMI info for barcode ACGGGTCAGGCGTACA-1 contig 2 = CTCATTCCTG...
umi ACAGTCCCGT = 1964 reads: +394 validated
umi CGGCAGCCGG = 263 reads: +367 -17 +10 non-validated
umi TTCGACCCAA = 1483 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=529]
0-75 ==> 0-75 on |467|TRAV14/DV4|5'UTR| [len=75] (mis=3)
75-425 ==> 0-350 on |468|TRAV14/DV4|L-REGION+V-REGION| [len=350] (mis=5)
427-487 ==> 0-60 on |400|TRAJ16|J-REGION| [len=60] (mis=1)
487-529 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 4 umis using 258 reads
cdr3 = CAMREARFSDGQKLLF at 408, score = 7 + 6
umis assigned: [119, 247, 279, 468]
of which 4 are surviving nonsolos
reads assigned: 5512
start codons at 75, 165, 208, 229, 267, 295, 307, 399, 414, 436, 468
confident = true

TIG 2[bases=469]
0-23 ==> 51-74 on |591|TRBV2|5'UTR| [len=74] (mis=0)
23-370 ==> 0-347 on |592|TRBV2|L-REGION+V-REGION| [len=347] (mis=5)
366-417 ==> 0-51 on |544|TRBJ2-2|J-REGION| [len=51] (mis=5)
417-469 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 2 umis using 248 reads
cdr3 = CASRGVTGELFF at 353, score = 6 + 9
umis assigned: [41, 222, 479]
of which 3 are surviving nonsolos
reads assigned: 3620
start codons at 23, 40, 122, 234, 264, 291, 344
confident = true
now this is a cell
paired!

GGGGACTCAGCAATGTATTTCTGTGCAATGAGAGAGGCTCGGTTTTCAGATGGCCAGAAGCTGCTCTTTGCAAGGGGAACCATGTTAAAGGTGGATCTTA <==> CGGTCCACAAAGCTGGAGGACTCAGCCATGTACTTCTGTGCCAGCCGGGGCGTAACCGGGGAGCTGTTTTTTGGAGAAGGCTCTAGGCTGACCGTACTGG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.1170 = ACGGGTCAGTACCGGA-1

using 22 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.1174 = ACGGGTCAGTCGATAA-1

using 36 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.1178 = ACGGGTCAGTGTACGG-1

using 36 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.1181 = ACGGGTCAGTTGAGTA-1

using 76 reads

====================================================================================

graph has 54 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 1[74]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.1182 = ACGGGTCAGTTGCAGG-1

using 23 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.1183 = ACGGGTCAGTTTGCGT-1

using 31155 reads

====================================================================================

graph has 18953 edges initially, 749 edges after simplification

total ucounts = 1472
nonsolo ucounts = 1218[2^114, 3^92, 4^60, 5^73, 6^46, 7^34, 8^41, 9^27, 10^31, 11^30, 12^30, 13^27, 14^25, 15^24, 16^15, 17^30, 18^19, 19^16, 20^16, 21^12, 22^19, 23^13, 24^14, 25^13, 26^4, 27^9, 28^10, 29^7, 30^9, 31^9, 32^11, 33^8, 34^4, 35^3, 36^11, 37^7, 38^6, 39^7, 40^10, 41^4, 42, 43^10, 44, 45^6, 46^7, 47^5, 48^7, 49^4, 50^5, 51^11, 52^5, 53^11, 54^7, 55^5, 56^6, 57^5, 58^2, 59^8, 60^4, 61^6, 62^6, 63^3, 64^2, 65^4, 66, 67^7, 68^4, 69^7, 70^6, 71^7, 72^2, 73^8, 74^4, 75^6, 76^6, 77^4, 78^2, 79^3, 80^6, 81^3, 82^4, 83^4, 84^5, 85^7, 86^3, 87^2, 88^2, 89^2, 90^2, 91^4, 93, 95^3, 97^2, 98^4, 99^3, 102, 103^3, 105^2, 106, 108, 110, 111^2, 112^2, 113, 114, 115, 116^2, 118^2, 119, 122, 129]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.1184 = ACGGGTCCAAACGTGG-1

using 73 reads

====================================================================================

graph has 62 edges initially, 4 edges after simplification

total ucounts = 5
nonsolo ucounts = 2[29, 41]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.1185 = ACGGGTCCAACACCTA-1

using 55 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[55]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.1193 = ACGGGTCCAATCACAC-1

using 19 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.1194 = ACGGGTCCAATCTACG-1

using 29 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[6, 23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.1198 = ACGGGTCCACATCTTT-1

using 36 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.1202 = ACGGGTCCACCCAGTG-1

using 22 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.1206 = ACGGGTCCACGAAATA-1

using 86 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[86]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 8.1209 = ACGGGTCCACGTCTCT-1

using 70 reads

====================================================================================

graph has 69 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 3[4, 15, 48]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!
sorting bam, mem = 0.20
running samtools sort -l 8G -o /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_T_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk08-ucc6a7a72e6/files/contig_bam_sorted.bam /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_T_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk08-ucc6a7a72e6/files/contig_bam.bam

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108.488 seconds used processing barcodes, peak mem = 0.34
