[stdout]

starting vdj_asm_asm, mem = 0.01
available mem = 833.30 GB
/scratch/etanis/software/cellranger-6.1.2/lib/bin/cr_vdj martian assembly main /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GE...
n50_n50_rpu = 288

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 107.0 = TTCGGTCAGAGCTTCT-1

using 1998 reads

====================================================================================

graph has 2463 edges initially, 46 edges after simplification

total ucounts = 1013
nonsolo ucounts = 378[2^163, 3^88, 4^45, 5^27, 6^19, 7^15, 8^9, 9, 10^3, 11^5, 14, 16, 43]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 107.8 = TTCGGTCAGCAGACTG-1

using 220 reads

====================================================================================

graph has 257 edges initially, 4 edges after simplification

total ucounts = 37
nonsolo ucounts = 26[2, 3^3, 4^2, 5^3, 6^2, 7^4, 8^2, 10, 11^2, 12^2, 15^3, 18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 107.15 = TTCGGTCAGCGTTCCG-1

using 310 reads

====================================================================================

graph has 118 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[2, 306]
surviving nonsolo ucounts = 1[306]
ids = [0]

====================================================================================

UMI info for barcode TTCGGTCAGCGTTCCG-1 contig 1 = GGAGGAACTG...
umi CCGGCAATTA = 273 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=491]
0-34 ==> 13-47 on |278|IGKV3-11|5'UTR| [len=47] (mis=0)
34-379 ==> 0-345 on |279|IGKV3-11|L-REGION+V-REGION| [len=345] (mis=3)
379-416 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=0)
416-491 ==> 0-75 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 47 reads
cdr3 = CQQRSNWPLTF at 355, score = 9 + 9
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 268
start codons at 34, 242, 458
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 107.70 = TTCGGTCCAGATTGCT-1

using 264 reads

====================================================================================

graph has 132 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 3[6, 7, 245]
surviving nonsolo ucounts = 1[245]
ids = [0]

====================================================================================

UMI info for barcode TTCGGTCCAGATTGCT-1 contig 1 = AGGAGTCAGA...
umi AACATAGGCC = 245 reads: +388 validated

GOOD CONTIGS
... 49491 lines elided ...
start codons at 33, 82, 241, 340, 381, 463
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 107.3394 = TTTGTCACATTATCTC-1

using 251 reads

====================================================================================

graph has 84 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 2[2, 244]
surviving nonsolo ucounts = 1[244]
ids = [4]

====================================================================================

UMI info for barcode TTTGTCACATTATCTC-1 contig 1 = GGGGAAGCTC...
umi TAGCGTCGCA = 234 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=543]
0-57 ==> 57-114 on |322|IGLV1-44|5'UTR| [len=114] (mis=0)
57-410 ==> 0-353 on |323|IGLV1-44|L-REGION+V-REGION| [len=353] (mis=15)
407-445 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=3)
445-543 ==> 0-98 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 44 reads
cdr3 = CASWDDSLRGRVF at 378, score = 8 + 8
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 233
start codons at 57, 211, 361, 386, 391
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 107.3399 = TTTGTCACATTTGCTT-1

using 320 reads

====================================================================================

graph has 104 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[319]
surviving nonsolo ucounts = 1[319]
ids = [0]

====================================================================================

UMI info for barcode TTTGTCACATTTGCTT-1 contig 1 = GAGCTCTGGG...
umi CGAATTATCG = 286 reads: +433 validated

GOOD CONTIGS

TIG 1[bases=540]
0-80 ==> 0-80 on |125|IGHV3-33|5'UTR| [len=80] (mis=1)
80-431 ==> 0-351 on |126|IGHV3-33|L-REGION+V-REGION| [len=351] (mis=0)
465-513 ==> 0-48 on |55|IGHJ4|J-REGION| [len=48] (mis=2)
513-540 ==> 0-27 on |60|IGHM|C-REGION| [len=1358] (mis=0)
junction support: 1 umis using 16 reads
cdr3 = CARDGWGYGGNSVNWYFDYW at 422, score = 9 + 7
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 283
start codons at 80, 231, 236, 289, 294, 297, 315, 383, 432
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 107.3401 = TTTGTCAGTAATCGTC-1

using 29 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 12
nonsolo ucounts = 8[2^3, 3, 4^4]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 107.3407 = TTTGTCAGTAGTACCT-1

using 533 reads

====================================================================================

graph has 244 edges initially, 4 edges after simplification

total ucounts = 25
nonsolo ucounts = 13[2^3, 3^2, 4^4, 5, 19, 230, 239]
surviving nonsolo ucounts = 2[230, 239]
ids = [12, 6]

====================================================================================

UMI info for barcode TTTGTCAGTAGTACCT-1 contig 1 = ACAAGAGGCA...
umi CGCAGACGTG = 221 reads: +391 validated

UMI info for barcode TTTGTCAGTAGTACCT-1 contig 2 = ATGGGAAGTG...
umi GCCACAGGGA = 218 reads: +439 validated

GOOD CONTIGS

TIG 1[bases=584]
0-31 ==> 20-51 on |320|IGLV1-40|5'UTR| [len=51] (mis=0)
31-387 ==> 0-356 on |321|IGLV1-40|L-REGION+V-REGION| [len=356] (mis=12)
384-422 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=3)
422-584 ==> 0-162 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 53 reads
cdr3 = CQSYDTSLSGSVF at 355, score = 8 + 8
umis assigned: [6]
of which 1 are surviving nonsolos
reads assigned: 218
start codons at 31, 185, 188, 239, 338, 365
confident = false

TIG 2[bases=584]
0-23 ==> 8-31 on |185|IGHV4-34|5'UTR| [len=31] (mis=0)
23-394 ==> 0-371 on |186|IGHV4-34|L-REGION+V-REGION| [len=371] (mis=30)
412-462 ==> 0-50 on |53|IGHJ3|J-REGION| [len=50] (mis=2)
462-584 ==> 0-122 on |40|IGHG1|C-REGION| [len=884] (mis=1)
junction support: 1 umis using 27 reads
cdr3 = CTRDATSSWDDAFDVW at 383, score = 9 + 7
umis assigned: [12]
of which 1 are surviving nonsolos
reads assigned: 215
start codons at 0, 23, 44, 88, 189, 411, 414, 423
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 107.3413 = TTTGTCAGTCAGTGGA-1

using 756 reads

====================================================================================

graph has 718 edges initially, 8 edges after simplification

total ucounts = 163
nonsolo ucounts = 70[2^22, 3^17, 4^8, 5^8, 6^3, 7^3, 9^2, 11, 13^3, 16, 124, 248]
surviving nonsolo ucounts = 1[248]
ids = [3]

====================================================================================

UMI info for barcode TTTGTCAGTCAGTGGA-1 contig 1 = ACAACAGGCA...
umi AATCGTCGTC = 235 reads: +400 validated

GOOD CONTIGS

TIG 1[bases=486]
0-25 ==> 150-175 on |295|IGKV4-1|5'UTR| [len=175] (mis=0)
25-388 ==> 0-363 on |296|IGKV4-1|L-REGION+V-REGION| [len=363] (mis=12)
387-425 ==> 0-38 on |217|IGKJ5|J-REGION| [len=38] (mis=5)
425-486 ==> 0-61 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 37 reads
cdr3 = CQQYYSSPQTF at 364, score = 9 + 8
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 231
start codons at 25, 94, 347, 467
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 107.3419 = TTTGTCAGTGAAATCA-1

using 1219 reads

====================================================================================

graph has 468 edges initially, 20 edges after simplification

total ucounts = 8
nonsolo ucounts = 3[242, 298, 674]
surviving nonsolo ucounts = 3[242, 298, 674]
ids = [3, 1, 7]

====================================================================================

UMI info for barcode TTTGTCAGTGAAATCA-1 contig 1 = AAAAACCACA...
umi CGGTGGACTA = 223 reads: +436 validated

UMI info for barcode TTTGTCAGTGAAATCA-1 contig 2 = GGAGGAATCA...
umi TCCATCTTGA = 673 reads: +22 -1XX +365 invalidated

UMI info for barcode TTTGTCAGTGAAATCA-1 contig 3 = TGGGGAGTCA...
umi ACGTTATCGT = 299 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=574]
0-50 ==> 9-59 on |64|IGHV1-18|5'UTR| [len=59] (mis=1)
50-403 ==> 0-353 on |65|IGHV1-18|L-REGION+V-REGION| [len=353] (mis=22)
452-486 ==> 12-46 on |54|IGHJ4|J-REGION| [len=46] (mis=0)
486-574 ==> 0-88 on |1|IGHA1|C-REGION| [len=1058] (mis=0)
junction support: 1 umis using 29 reads
cdr3 = CARDKHFRDPYYHYSGALDHW at 392, score = 7 + 7
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 220
start codons at 50, 248, 253, 270, 314, 347, 540
confident = false

TIG 2[bases=553]
29-380 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=18)
379-417 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
417-553 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 128 reads
cdr3 = CLQYSSSPWTF at 356, score = 9 + 8
umis assigned: [7]
of which 1 are surviving nonsolos
reads assigned: 658
start codons at 29, 35, 104, 240, 459
confident = false

TIG 3[bases=553]
0-29 ==> 0-29 on |234|IGKV1-6|5'UTR| [len=29] (mis=3)
29-380 ==> 0-351 on |235|IGKV1-6|L-REGION+V-REGION| [len=351] (mis=28)
378-417 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=5)
417-553 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 49 reads
cdr3 = CLQDYTYPFSF at 356, score = 9 + 7
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 293
start codons at 29, 35, 91, 104, 186, 189, 459
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 107.3429 = TTTGTCAGTTCTCATT-1

using 446 reads

====================================================================================

graph has 86 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 3[4, 164, 270]
surviving nonsolo ucounts = 2[164, 270]
ids = [3, 9]

====================================================================================

UMI info for barcode TTTGTCAGTTCTCATT-1 contig 1 = GGTAGCTCAG...
umi CCATAACCGT = 162 reads: +388 validated
umi GTTGCAGCAG = 268 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=635]
0-36 ==> 50-86 on |732|IGLV6-57|5'UTR| [len=86] (mis=0)
36-389 ==> 0-353 on |384|IGLV6-57|L-REGION+V-REGION| [len=353] (mis=12)
388-424 ==> 10-46 on |317|IGLJ7|J-REGION| [len=46] (mis=0)
424-635 ==> 0-211 on |308|IGLC7|C-REGION| [len=317] (mis=2)
junction support: 2 umis using 96 reads
cdr3 = CQSYDSSSTVF at 363, score = 6 + 7
umis assigned: [3, 9]
of which 2 are surviving nonsolos
reads assigned: 426
start codons at 36, 99, 190, 241, 247, 373, 556, 619
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 107.3432 = TTTGTCATCAAGGTAA-1

using 12 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[12]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 107.3437 = TTTGTCATCACTTATC-1

using 47 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 15
nonsolo ucounts = 9[3^3, 4^2, 5^2, 6, 8]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 107.3438 = TTTGTCATCAGCCTAA-1

using 365 reads

====================================================================================

graph has 138 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 2[2, 358]
surviving nonsolo ucounts = 1[358]
ids = [1]

====================================================================================

UMI info for barcode TTTGTCATCAGCCTAA-1 contig 1 = GCTCTGCTTC...
umi CCCCTCACTT = 348 reads: +391 validated

GOOD CONTIGS

TIG 1[bases=585]
0-51 ==> 0-51 on |320|IGLV1-40|5'UTR| [len=51] (mis=0)
51-407 ==> 0-356 on |321|IGLV1-40|L-REGION+V-REGION| [len=356] (mis=12)
404-442 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=3)
442-585 ==> 0-143 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 58 reads
cdr3 = CQSYDTSLSGSVF at 375, score = 8 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 340
start codons at 51, 205, 208, 259, 358, 385
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 107.3448 = TTTGTCATCCGCATCT-1

using 324 reads

====================================================================================

graph has 100 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 2[3, 316]
surviving nonsolo ucounts = 1[316]
ids = [6]

====================================================================================

UMI info for barcode TTTGTCATCCGCATCT-1 contig 1 = AGGAATCAGA...
umi TTTGTTAGCT = 316 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=551]
0-27 ==> 0-27 on |220|IGKV1-16|5'UTR| [len=27] (mis=0)
27-358 ==> 0-331 on |221|IGKV1-16|L-REGION+V-REGION| [len=351] (mis=8)
378-415 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=2)
415-551 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 58 reads
cdr3 = CLQYDTYPLTF at 354, score = 9 + 9
umis assigned: [6]
of which 1 are surviving nonsolos
reads assigned: 310
start codons at 27, 33, 89, 102, 364, 457
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 107.3455 = TTTGTCATCGAACGGA-1

using 273 reads

====================================================================================

graph has 112 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 4[2, 4^2, 257]
surviving nonsolo ucounts = 1[257]
ids = [0]

====================================================================================

UMI info for barcode TTTGTCATCGAACGGA-1 contig 1 = GATCAGGACT...
umi AAGAGCGTAC = 256 reads: +400 validated

GOOD CONTIGS

TIG 1[bases=566]
0-30 ==> 0-30 on |264|IGKV2-28|5'UTR| [len=30] (mis=0)
30-390 ==> 0-360 on |265|IGKV2-28|L-REGION+V-REGION| [len=360] (mis=1)
392-430 ==> 0-38 on |215|IGKJ3|J-REGION| [len=38] (mis=0)
430-566 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 27 reads
cdr3 = CMQALQTPLFTF at 366, score = 9 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 254
start codons at 30, 63, 99, 187, 349, 369, 472
confident = false
NOT paired!

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BARCODE 107.3458 = TTTGTCATCGCAAACT-1

using 84 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 4[2, 3, 4, 72]
surviving nonsolo ucounts = 1[72]
ids = [1]

====================================================================================

REJECT CONTIGS

TIG 1[bases=335]
0-310 ==> 41-351 on |219|IGKV1-12|L-REGION+V-REGION| [len=351] (mis=25)
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 67
start codons at 21, 34
confident = false
did not find CDR3
NOT paired!

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BARCODE 107.3459 = TTTGTCATCGCCTGAG-1

using 94 reads

====================================================================================

graph has 84 edges initially, 2 edges after simplification

total ucounts = 23
nonsolo ucounts = 10[2^3, 3, 5, 6, 7, 8, 13, 33]
surviving nonsolo ucounts = 1[33]
ids = [2]

====================================================================================
NOT paired!

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BARCODE 107.3467 = TTTGTCATCTTCCTTC-1

using 41 reads

====================================================================================

graph has 55 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 2[4, 34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!
sorting bam, mem = 0.21
running samtools sort -l 8G -o /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ...

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232.206 seconds used processing barcodes, peak mem = 0.34
