[stdout]

starting vdj_asm_asm, mem = 0.01
available mem = 834.27 GB
/scratch/etanis/software/cellranger-6.1.2/lib/bin/cr_vdj martian assembly main /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk084-ucc6a7a72ce /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk084-ucc6a7a72ce/files /scratch/etanis/ST-118/multi/dimitri-multi/journal/SC_MULTI_CS.SC_MULTI_CORE.MULTI_GEM_WELL_PROCESSOR.VDJ_B_GEM_WELL_PROCESSOR.SC_VDJ_CONTIG_ASSEMBLER.ASSEMBLE_VDJ.fork0.chnk084.ucc6a7a72ce
n50_n50_rpu = 288

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.0 = GTCGTAACACGGATAG-1

using 113 reads

====================================================================================

graph has 54 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[113]
surviving nonsolo ucounts = 1[113]
ids = [0]

====================================================================================

UMI info for barcode GTCGTAACACGGATAG-1 contig 1 = AGTCTCAGTC...
umi GACTAACTTT = 101 reads: +391 validated

GOOD CONTIGS

TIG 1[bases=462]
0-20 ==> 3-23 on |252|IGKV1D-39|5'UTR| [len=23] (mis=0)
20-373 ==> 0-353 on |253|IGKV1D-39|L-REGION+V-REGION| [len=353] (mis=3)
373-411 ==> 0-38 on |215|IGKJ3|J-REGION| [len=38] (mis=0)
411-462 ==> 0-51 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 13 reads
cdr3 = CQQSYSTLLFTF at 347, score = 9 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 100
start codons at 20, 26, 82, 95, 231, 453
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.1 = GTCGTAACACGGTAGA-1

using 101 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 1[99]
surviving nonsolo ucounts = 1[99]
ids = [0]

====================================================================================

UMI info for barcode GTCGTAACACGGTAGA-1 contig 1 = GGGGTGTCTC...
umi CCTGTGGGCA = 94 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=491]
14-351 ==> 0-337 on |357|IGLV3-19|L-REGION+V-REGION| [len=337] (mis=0)
358-396 ==> 0-38 on |309|IGLJ1|J-REGION| [len=38] (mis=0)
396-491 ==> 0-95 on |305|IGLC1|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 17 reads
cdr3 = CNSRDSSGNHYVF at 329, score = 8 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 92
start codons at 14, 133, 162, 213, 312, 360
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.12 = GTCGTAACATCCGGGT-1

using 188 reads

====================================================================================

graph has 66 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[74, 114]
surviving nonsolo ucounts = 2[74, 114]
ids = [1, 0]

====================================================================================

REJECT CONTIGS

TIG 1[bases=493]
0-33 ==> 19-52 on |282|IGKV3-20|5'UTR| [len=52] (mis=0)
0-34 ==> 5703-5737 on segment before IGKV3OR2-5 exon 1 [len=6000] (mis=1)
33-381 ==> 0-348 on |283|IGKV3-20|L-REGION+V-REGION| [len=348] (mis=9)
379-418 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=6)
418-493 ==> 0-75 on |212|IGKC|C-REGION| [len=320] (mis=0)
cdr3 = CQQYGCSPGTF at 357, score = 9 + 8
umis assigned: [0, 1]
of which 2 are surviving nonsolos
reads assigned: 169
start codons at 33, 241, 367, 460
confident = false
not full
full length stopped transcript of length 493
frameshifted full length stopped transcript of length 493
VJ delta = 15
not full
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.13 = GTCGTAACATGAAGTA-1

using 64 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[64]
surviving nonsolo ucounts = 1[64]
ids = [0]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.15 = GTCGTAACATGTAGTC-1

using 1803 reads

====================================================================================

graph has 1756 edges initially, 14 edges after simplification

total ucounts = 441
nonsolo ucounts = 309[2^60, 3^48, 4^36, 5^42, 6^33, 7^25, 8^18, 9^11, 10^11, 11^6, 12^5, 13^6, 14^4, 15^2, 17, 20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.16 = GTCGTAACATGTCTCC-1

using 2024 reads

====================================================================================

graph has 1278 edges initially, 26 edges after simplification

total ucounts = 248
nonsolo ucounts = 93[2^44, 3^19, 4^4, 5^3, 6^2, 11, 15, 23, 61, 68, 72^2, 75, 77, 83, 85, 87^2, 88, 92^2, 96, 105, 106, 107, 179]
surviving nonsolo ucounts = 20[3, 23, 61, 68, 72^2, 75, 77, 83, 85, 87^2, 88, 92^2, 96, 105, 106, 107, 179]
ids = [235, 13, 190, 95, 90, 159, 133, 191, 46, 97, ...]

====================================================================================

UMI info for barcode GTCGTAACATGTCTCC-1 contig 1 = GTGGGCTCAG...
umi AATCAGGCCC = 23 reads: +4 -1 +2 -1 +22 -1X +84 -1 +222 -20 +27 invalidated
umi ATATGTCTCC = 86 reads: +385 validated
umi CACAACCTTC = 108 reads: +289 -2XX +94 invalidated
umi CCTCGACGAA = 93 reads: +385 validated
umi GGGTCAGGAC = 111 reads: +385 validated
umi GTATGGCCTC = 73 reads: +385 validated
umi TCCGATTCTG = 77 reads: +385 validated
umi TTCCTGGGCT = 106 reads: +385 validated

GOOD CONTIGS

TIG 1[bases=631]
0-35 ==> 0-35 on |350|IGLV3-10|5'UTR| [len=35] (mis=0)
35-382 ==> 0-347 on |351|IGLV3-10|L-REGION+V-REGION| [len=347] (mis=2)
382-420 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=2)
420-631 ==> 0-211 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 6 umis using 92 reads
cdr3 = CYSTDSSGNHRGVF at 350, score = 7 + 8
umis assigned: [13, 60, 78, 98, 152, 159, 191, 226]
of which 8 are surviving nonsolos
reads assigned: 662
start codons at 35, 96, 165, 183, 234, 296, 333
confident = true

REJECT CONTIGS

TIG 1[bases=406]
0-20 ==> 1876-1896 on segment before IGHVIII-67-4 exon 1 [len=2213] (mis=0)
0-20 ==> 1877-1897 on segment before IGHVIII-67-4 exon 1 [len=2213] (mis=0)
17-264 ==> 106-353 on |159|IGHV3-7|L-REGION+V-REGION| [len=353] (mis=2)
284-335 ==> 0-51 on |57|IGHJ5|J-REGION| [len=51] (mis=1)
335-406 ==> 0-71 on |60|IGHM|C-REGION| [len=1358] (mis=0)
cdr3 = CARDPDYGESENWFDPW at 253, score = 9 + 7
umis assigned: [46, 71, 79, 97]
of which 4 are surviving nonsolos
reads assigned: 257
start codons at 67, 128, 146, 214
confident = false
VJ delta = 11
not full
not full

TIG 2[bases=430]
0-80 ==> 3254-3334 on rc of segment before IGKV2-24 exon 2 [len=3774] (mis=5)
0-80 ==> 3251-3331 on segment before IGKV2D-23 exon 1 [len=3772] (mis=5)
30-378 ==> 0-348 on |265|IGKV2-28|L-REGION+V-REGION| [len=360] (mis=35)
393-430 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=0)
cdr3 = CMQATQFPPLTF at 366, score = 9 + 9
umis assigned: [173, 235]
of which 2 are surviving nonsolos
reads assigned: 81
start codons at 63, 99, 187, 349, 369
confident = false
not full
VJ delta = 13
not full

TIG 3[bases=639]
0-68 ==> 5924-5992 on segment before IGLV2-5 exon 1 [len=6137] (mis=4)
41-393 ==> 0-352 on |339|IGLV2-14|L-REGION+V-REGION| [len=361] (mis=2)
400-428 ==> 10-38 on |311|IGLJ2|J-REGION| [len=38] (mis=0)
428-639 ==> 0-211 on |306|IGLC2|C-REGION| [len=317] (mis=0)
umis assigned: [90, 95, 133, 170, 190, 229]
of which 6 are surviving nonsolos
reads assigned: 485
start codons at 41, 198, 242, 249
confident = false
did not find CDR3
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.18 = GTCGTAAGTAGAAGGA-1

using 240 reads

====================================================================================

graph has 98 edges initially, 4 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[46, 194]
surviving nonsolo ucounts = 2[46, 194]
ids = [1, 0]

====================================================================================

UMI info for barcode GTCGTAAGTAGAAGGA-1 contig 1 = CAGTCTCAGT...
umi TCATTCCTAC = 195 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=545]
0-21 ==> 2-23 on |252|IGKV1D-39|5'UTR| [len=23] (mis=0)
21-374 ==> 0-353 on |253|IGKV1D-39|L-REGION+V-REGION| [len=353] (mis=27)
371-409 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=6)
409-545 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 28 reads
cdr3 = CQHTYSPPRAF at 348, score = 9 + 7
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 193
start codons at 21, 27, 83, 96, 232, 451
confident = false

REJECT CONTIGS

TIG 1[bases=310]
0-305 ==> 6-311 on |740|IGHV4-30-4|L-REGION+V-REGION| [len=348] (mis=6)
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 31
start codons at 38
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.22 = GTCGTAAGTCACTGGC-1

using 57 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 1[54]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================

REJECT CONTIGS

TIG 1[bases=352]
2-60 ==> 4685-4743 on rc of segment before IGHVII-28-1 exon 1 [len=4743] (mis=0)
60-333 ==> 0-273 on |740|IGHV4-30-4|L-REGION+V-REGION| [len=348] (mis=11)
umis assigned: [3]
of which 0 are surviving nonsolos
reads assigned: 42
start codons at 16, 60, 104, 260, 339
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.25 = GTCGTAAGTCCTCCAT-1

using 409 reads

====================================================================================

graph has 156 edges initially, 6 edges after simplification

total ucounts = 4
nonsolo ucounts = 4[51, 79, 110, 169]
surviving nonsolo ucounts = 4[51, 79, 110, 169]
ids = [0, 2, 1, 3]

====================================================================================

UMI info for barcode GTCGTAAGTCCTCCAT-1 contig 1 = TTGCTGATCA...
umi AAATCAGTTA = 46 reads: +409 validated

GOOD CONTIGS

TIG 1[bases=445]
0-33 ==> 203-236 on |168|IGHV3-74|5'UTR| [len=236] (mis=0)
33-364 ==> 0-331 on |169|IGHV3-74|L-REGION+V-REGION| [len=351] (mis=17)
405-442 ==> 26-63 on |737|IGHJ6|J-REGION| [len=63] (mis=0)
junction support: 1 umis using 11 reads
cdr3 = CTNLYHFGSEVW at 375, score = 9 + 7
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 45
start codons at 33, 189, 250
confident = true
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.31 = GTCGTAAGTGATGATA-1

using 48 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[2, 44]
surviving nonsolo ucounts = 1[44]
ids = [0]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.35 = GTCGTAAGTGGTAACG-1

using 49 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 1[47]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.38 = GTCGTAAGTGTTCTTT-1

using 189 reads

====================================================================================

graph has 92 edges initially, 2 edges after simplification

total ucounts = 12
nonsolo ucounts = 9[2^3, 3^3, 4, 7, 160]
surviving nonsolo ucounts = 1[160]
ids = [1]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.40 = GTCGTAAGTTCCACTC-1

using 121 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 1[115]
surviving nonsolo ucounts = 1[115]
ids = [4]

====================================================================================

UMI info for barcode GTCGTAAGTTCCACTC-1 contig 1 = TGGGGTCACA...
umi TAAATGTGCT = 105 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=512]
0-39 ==> 123-162 on |342|IGLV2-23|5'UTR| [len=162] (mis=0)
39-379 ==> 0-340 on |343|IGLV2-23|L-REGION+V-REGION| [len=340] (mis=10)
389-427 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=3)
427-512 ==> 0-85 on |307|IGLC3|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 19 reads
cdr3 = CCSYATSSSWVF at 363, score = 8 + 8
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 104
start codons at 39, 178, 247, 373
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.43 = GTCGTAAGTTCGCGAC-1

using 79 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[78]
surviving nonsolo ucounts = 1[78]
ids = [0]

====================================================================================

UMI info for barcode GTCGTAAGTTCGCGAC-1 contig 1 = AGACCCAGTC...
umi CATGTTCGGT = 73 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=445]
0-20 ==> 161-181 on |232|IGKV1-5|5'UTR| [len=181] (mis=0)
20-371 ==> 0-351 on |233|IGKV1-5|L-REGION+V-REGION| [len=351] (mis=0)
370-408 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=3)
408-445 ==> 0-37 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 19 reads
cdr3 = CQQYNSYSPTF at 347, score = 8 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 72
start codons at 20, 26, 82, 95, 327
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.47 = GTCGTAAGTTTGTTGG-1

using 143 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[62, 81]
surviving nonsolo ucounts = 2[62, 81]
ids = [0, 1]

====================================================================================

UMI info for barcode GTCGTAAGTTTGTTGG-1 contig 1 = GAGCTACAAC...
umi CAACGCATTG = 62 reads: +400 validated
umi TAACATTGGT = 77 reads: +400 validated

GOOD CONTIGS

TIG 1[bases=483]
0-30 ==> 145-175 on |295|IGKV4-1|5'UTR| [len=175] (mis=0)
30-393 ==> 0-363 on |296|IGKV4-1|L-REGION+V-REGION| [len=363] (mis=23)
393-430 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=0)
430-483 ==> 0-53 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 2 umis using 22 reads
cdr3 = CQQYFSIPLTF at 369, score = 9 + 9
umis assigned: [0, 1]
of which 2 are surviving nonsolos
reads assigned: 138
start codons at 30, 79, 99, 352, 472
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.51 = GTCGTAATCACTGGGC-1

using 243 reads

====================================================================================

graph has 222 edges initially, 6 edges after simplification

total ucounts = 45
nonsolo ucounts = 30[2^7, 3^4, 4^3, 5^2, 6^7, 7, 8^3, 15, 17, 75]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.57 = GTCGTAATCCAATGGT-1

using 501 reads

====================================================================================

graph has 874 edges initially, 4 edges after simplification

total ucounts = 295
nonsolo ucounts = 102[2^60, 3^15, 4^16, 5^2, 6^4, 7, 8, 9, 10, 11]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.69 = GTCGTAATCGTAGGTT-1

using 72 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 3[2, 3, 67]
surviving nonsolo ucounts = 1[67]
ids = [1]

====================================================================================

REJECT CONTIGS

TIG 1[bases=341]
0-35 ==> 17-52 on |282|IGKV3-20|5'UTR| [len=52] (mis=0)
0-35 ==> 5965-6000 on rc of segment after IGKV3-20 exon 1 [len=6000] (mis=0)
35-340 ==> 0-305 on |283|IGKV3-20|L-REGION+V-REGION| [len=348] (mis=3)
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 52
start codons at 35, 243
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.71 = GTCGTAATCTACCTGC-1

using 110 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[2, 106]
surviving nonsolo ucounts = 1[106]
ids = [0]

====================================================================================

UMI info for barcode GTCGTAATCTACCTGC-1 contig 1 = GAGGAACTGC...
umi ATCGAACTCT = 96 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=485]
0-33 ==> 64-97 on |280|IGKV3-15|5'UTR| [len=97] (mis=1)
33-378 ==> 0-345 on |281|IGKV3-15|L-REGION+V-REGION| [len=345] (mis=15)
376-415 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=3)
415-485 ==> 0-70 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 20 reads
cdr3 = CQQYNYWPYTF at 354, score = 9 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 92
start codons at 33, 102, 457
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.75 = GTCGTAATCTCGATGA-1

using 161 reads

====================================================================================

graph has 54 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[161]
surviving nonsolo ucounts = 1[161]
ids = [0]

====================================================================================

UMI info for barcode GTCGTAATCTCGATGA-1 contig 1 = AGTCTCAGTC...
umi GTGGGTATAG = 147 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=457]
0-20 ==> 3-23 on |252|IGKV1D-39|5'UTR| [len=23] (mis=0)
20-373 ==> 0-353 on |253|IGKV1D-39|L-REGION+V-REGION| [len=353] (mis=3)
369-408 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=3)
408-457 ==> 0-49 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 22 reads
cdr3 = CQQSYSTPYTF at 347, score = 9 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 146
start codons at 20, 26, 82, 95, 231, 450
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.76 = GTCGTAATCTCTTATG-1

using 5524 reads

====================================================================================

graph has 4885 edges initially, 121 edges after simplification

total ucounts = 1441
nonsolo ucounts = 971[2^231, 3^169, 4^134, 5^106, 6^83, 7^42, 8^59, 9^39, 10^26, 11^35, 12^13, 13^17, 14^7, 15^4, 16, 17, 18^2, 19, 136]
surviving nonsolo ucounts = 1[136]
ids = [342]

====================================================================================

UMI info for barcode GTCGTAATCTCTTATG-1 contig 1 = GAGCATCACC...
umi CCAAGAGGCT = 134 reads: +424 validated

GOOD CONTIGS

TIG 1[bases=557]
0-62 ==> 2-64 on |66|IGHV1-2|5'UTR| [len=64] (mis=0)
62-415 ==> 0-353 on |67|IGHV1-2|L-REGION+V-REGION| [len=353] (mis=1)
416-437 ==> 0-21 on |33|IGHD6-19|D-REGION| [len=21] (mis=3)
435-486 ==> 0-51 on |57|IGHJ5|J-REGION| [len=51] (mis=3)
486-557 ==> 0-71 on |60|IGHM|C-REGION| [len=1358] (mis=0)
junction support: 1 umis using 7 reads
cdr3 = CARDGGSSGWYGWFDPW at 404, score = 8 + 7
umis assigned: [342]
of which 1 are surviving nonsolos
reads assigned: 133
start codons at 62, 218, 260, 265, 297, 326, 359, 414, 439
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.80 = GTCGTAATCTTCGAGA-1

using 89 reads

====================================================================================

graph has 48 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 1[86]
surviving nonsolo ucounts = 1[86]
ids = [3]

====================================================================================

UMI info for barcode GTCGTAATCTTCGAGA-1 contig 1 = GCTGTGGGTA...
umi GCTCGATTTC = 77 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=513]
0-40 ==> 74-114 on |322|IGLV1-44|5'UTR| [len=114] (mis=0)
40-393 ==> 0-353 on |323|IGLV1-44|L-REGION+V-REGION| [len=353] (mis=15)
390-428 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=3)
428-513 ==> 0-85 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 17 reads
cdr3 = CASWDDSLRGRVF at 361, score = 8 + 8
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 76
start codons at 40, 194, 344, 369, 374
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.91 = GTCTCGTAGCCAACAG-1

using 86 reads

====================================================================================

graph has 58 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 4[2, 6^2, 65]
surviving nonsolo ucounts = 1[65]
ids = [1]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.94 = GTCTCGTAGCTCAACT-1

using 24 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 17
nonsolo ucounts = 4[2^2, 3, 4]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.100 = GTCTCGTAGGCAAAGA-1

using 207 reads

====================================================================================

graph has 216 edges initially, 2 edges after simplification

total ucounts = 149
nonsolo ucounts = 36[2^26, 3^6, 4^2, 6, 10]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.110 = GTCTCGTCAACGCACC-1

using 115 reads

====================================================================================

graph has 154 edges initially, 2 edges after simplification

total ucounts = 29
nonsolo ucounts = 19[2^5, 3^3, 4^3, 6, 7^2, 8, 9, 11, 12, 14]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.111 = GTCTCGTCAACTGCGC-1

using 382 reads

====================================================================================

graph has 122 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 6[2, 3^3, 4, 365]
surviving nonsolo ucounts = 1[365]
ids = [0]

====================================================================================

UMI info for barcode GTCTCGTCAACTGCGC-1 contig 1 = GAGAAGAGCT...
umi ACTGCCTCTG = 365 reads: +385 validated

GOOD CONTIGS

TIG 1[bases=556]
0-35 ==> 17-52 on |282|IGKV3-20|5'UTR| [len=52] (mis=0)
35-383 ==> 0-348 on |283|IGKV3-20|L-REGION+V-REGION| [len=348] (mis=2)
382-420 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=0)
420-556 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 50 reads
cdr3 = CQQYGSSLWTF at 359, score = 9 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 359
start codons at 35, 243, 369, 462
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.123 = GTCTCGTCACACCGAC-1

using 41 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 5[2, 3, 5, 12, 14]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.124 = GTCTCGTCACAGTCGC-1

using 212 reads

====================================================================================

graph has 98 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 7[2^5, 4, 195]
surviving nonsolo ucounts = 1[195]
ids = [9]

====================================================================================

UMI info for barcode GTCTCGTCACAGTCGC-1 contig 1 = GCTCTGCTTC...
umi TTTACCACGG = 190 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=558]
0-51 ==> 0-51 on |320|IGLV1-40|5'UTR| [len=51] (mis=0)
51-407 ==> 0-356 on |321|IGLV1-40|L-REGION+V-REGION| [len=356] (mis=12)
407-445 ==> 0-38 on |309|IGLJ1|J-REGION| [len=38] (mis=1)
445-558 ==> 0-113 on |305|IGLC1|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 26 reads
cdr3 = CQSYDSSLSGLYVF at 375, score = 7 + 8
umis assigned: [9]
of which 1 are surviving nonsolos
reads assigned: 188
start codons at 51, 205, 208, 259, 358, 385, 409
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.127 = GTCTCGTCACCAGGCT-1

using 152 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 2[2, 146]
surviving nonsolo ucounts = 1[146]
ids = [2]

====================================================================================

UMI info for barcode GTCTCGTCACCAGGCT-1 contig 1 = AGTCCCAGTC...
umi GAGAGCGTCA = 131 reads: +314 -3XX +1 -2XX +1 -1 +66 invalidated

GOOD CONTIGS

TIG 1[bases=420]
0-20 ==> 38-58 on |238|IGKV1-9|5'UTR| [len=58] (mis=0)
20-371 ==> 0-351 on |239|IGKV1-9|L-REGION+V-REGION| [len=351] (mis=1)
371-408 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=0)
junction support: 1 umis using 31 reads
cdr3 = CQQLNSYPLTF at 347, score = 9 + 9
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 127
start codons at 20, 26, 82, 231
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.128 = GTCTCGTCACCGAAAG-1

using 530 reads

====================================================================================

graph has 170 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 2[2, 522]
surviving nonsolo ucounts = 1[522]
ids = [3]

====================================================================================

REJECT CONTIGS

TIG 1[bases=457]
3-192 ==> 151-340 on |343|IGLV2-23|L-REGION+V-REGION| [len=340] (mis=3)
208-246 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=1)
246-457 ==> 0-211 on |306|IGLC2|C-REGION| [len=317] (mis=0)
cdr3 = CCSYAGSRTPNWVF at 176, score = 8 + 8
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 511
start codons at 53, 60, 186
confident = false
not full
VJ delta = 6
not full
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.133 = GTCTCGTCAGACAGGT-1

using 20 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.134 = GTCTCGTCAGATCTGT-1

using 367 reads

====================================================================================

graph has 158 edges initially, 4 edges after simplification

total ucounts = 13
nonsolo ucounts = 11[2^2, 3^2, 5, 6^2, 12, 13, 48, 265]
surviving nonsolo ucounts = 1[265]
ids = [9]

====================================================================================

UMI info for barcode GTCTCGTCAGATCTGT-1 contig 1 = GGAGTCAGTC...
umi CTCGTTCCTC = 239 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=471]
26-379 ==> 0-353 on |253|IGKV1D-39|L-REGION+V-REGION| [len=353] (mis=13)
377-414 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=0)
414-471 ==> 0-57 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 37 reads
cdr3 = CQQNYTTLLTF at 353, score = 9 + 9
umis assigned: [9]
of which 1 are surviving nonsolos
reads assigned: 237
start codons at 26, 32, 88, 101, 237, 456
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.137 = GTCTCGTCAGCGAACA-1

using 10 reads

====================================================================================

graph has 4 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[10]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.139 = GTCTCGTCAGCTGTAT-1

using 26 reads

====================================================================================

graph has 30 edges initially, 6 edges after simplification

total ucounts = 12
nonsolo ucounts = 5[2^3, 3, 10]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.143 = GTCTCGTCAGTCGATT-1

using 171 reads

====================================================================================

graph has 106 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 4[2^2, 10, 152]
surviving nonsolo ucounts = 1[152]
ids = [7]

====================================================================================

UMI info for barcode GTCTCGTCAGTCGATT-1 contig 1 = GAGAAGAGCT...
umi TGGAACTGTA = 136 reads: +385 validated

GOOD CONTIGS

TIG 1[bases=434]
0-35 ==> 17-52 on |282|IGKV3-20|5'UTR| [len=52] (mis=0)
35-383 ==> 0-348 on |283|IGKV3-20|L-REGION+V-REGION| [len=348] (mis=1)
382-420 ==> 0-38 on |217|IGKJ5|J-REGION| [len=38] (mis=2)
junction support: 1 umis using 17 reads
cdr3 = CQQYGSSPLTF at 359, score = 9 + 9
umis assigned: [7]
of which 1 are surviving nonsolos
reads assigned: 134
start codons at 35, 243, 369
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.148 = GTCTCGTCATATACCG-1

using 298 reads

====================================================================================

graph has 126 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[2^2, 3, 289]
surviving nonsolo ucounts = 1[289]
ids = [1]

====================================================================================

REJECT CONTIGS

TIG 1[bases=469]
4-264 ==> 61-321 on |253|IGKV1D-39|L-REGION+V-REGION| [len=353] (mis=16)
266-298 ==> 321-353 on |253|IGKV1D-39|L-REGION+V-REGION| [len=353] (mis=5)
294-333 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=6)
333-469 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
cdr3 = CQQSYSTPYTF at 272, score = 5 + 7
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 283
start codons at 5, 18, 208, 375
confident = false
not full
VJ delta = 15
not full
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.152 = GTCTCGTCATGATCCA-1

using 221 reads

====================================================================================

graph has 126 edges initially, 2 edges after simplification

total ucounts = 13
nonsolo ucounts = 5[2^2, 3^2, 203]
surviving nonsolo ucounts = 1[203]
ids = [3]

====================================================================================

UMI info for barcode GTCTCGTCATGATCCA-1 contig 1 = GGTGTGGGGG...
umi ATGCTCCGCC = 206 reads: +403 validated

GOOD CONTIGS

TIG 1[bases=585]
0-28 ==> 0-28 on |380|IGLV5-45|5'UTR| [len=28] (mis=3)
28-397 ==> 0-369 on |381|IGLV5-45|L-REGION+V-REGION| [len=369] (mis=15)
393-431 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=2)
431-585 ==> 0-154 on |307|IGLC3|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 25 reads
cdr3 = CMIWHSSSWVF at 370, score = 8 + 7
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 198
start codons at 28, 89, 170, 302, 314, 353, 373
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.158 = GTCTCGTCATTTCACT-1

using 19 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 12
nonsolo ucounts = 5[2^4, 4]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.165 = GTCTCGTTCAATAAGG-1

using 67 reads

====================================================================================

graph has 83 edges initially, 2 edges after simplification

total ucounts = 22
nonsolo ucounts = 10[2^3, 3^2, 6, 7^2, 11, 12]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.179 = GTCTCGTTCCAATGGT-1

using 139 reads

====================================================================================

graph has 82 edges initially, 2 edges after simplification

total ucounts = 13
nonsolo ucounts = 3[2, 4, 123]
surviving nonsolo ucounts = 1[123]
ids = [8]

====================================================================================

UMI info for barcode GTCTCGTTCCAATGGT-1 contig 1 = AGCTTCAGCT...
umi CTTTTATGGA = 117 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=519]
0-47 ==> 67-114 on |322|IGLV1-44|5'UTR| [len=114] (mis=0)
47-400 ==> 0-353 on |323|IGLV1-44|L-REGION+V-REGION| [len=353] (mis=15)
397-435 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=3)
435-519 ==> 0-84 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 23 reads
cdr3 = CASWDDSLRGRVF at 368, score = 8 + 8
umis assigned: [8]
of which 1 are surviving nonsolos
reads assigned: 116
start codons at 47, 201, 351, 376, 381
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.183 = GTCTCGTTCCTCAATT-1

using 894 reads

====================================================================================

graph has 234 edges initially, 4 edges after simplification

total ucounts = 7
nonsolo ucounts = 6[2^3, 3, 286, 598]
surviving nonsolo ucounts = 2[286, 598]
ids = [6, 1]

====================================================================================

UMI info for barcode GTCTCGTTCCTCAATT-1 contig 1 = TGGGGAGGAA...
umi TTCGCGTGTA = 286 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=556]
32-383 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=7)
382-420 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=0)
420-556 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 57 reads
cdr3 = CLQHKNYPWTF at 359, score = 9 + 8
umis assigned: [6]
of which 1 are surviving nonsolos
reads assigned: 281
start codons at 32, 38, 107, 189, 243, 462
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.186 = GTCTCGTTCGAATGCT-1

using 250 reads

====================================================================================

graph has 92 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[5, 243]
surviving nonsolo ucounts = 1[243]
ids = [0]

====================================================================================

UMI info for barcode GTCTCGTTCGAATGCT-1 contig 1 = AGCTTCAGCT...
umi AGTTTTCGGT = 242 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=540]
0-47 ==> 67-114 on |322|IGLV1-44|5'UTR| [len=114] (mis=0)
47-400 ==> 0-353 on |323|IGLV1-44|L-REGION+V-REGION| [len=353] (mis=2)
397-435 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=0)
435-540 ==> 0-105 on |307|IGLC3|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 44 reads
cdr3 = CAAWDDSLNGWVF at 368, score = 8 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 239
start codons at 47, 351, 376, 381, 393
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.193 = GTCTCGTTCGTCCAGG-1

using 308 reads

====================================================================================

graph has 122 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 4[2^2, 5, 295]
surviving nonsolo ucounts = 1[295]
ids = [3]

====================================================================================

REJECT CONTIGS

TIG 1[bases=569]
0-52 ==> 0-52 on |348|IGLV3-1|5'UTR| [len=52] (mis=0)
25-57 ==> 5623-5655 on segment before IGLV3-29 exon 1 [len=6000] (mis=1)
52-398 ==> 0-346 on |349|IGLV3-1|L-REGION+V-REGION| [len=346] (mis=5)
394-432 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=0)
432-569 ==> 0-137 on |306|IGLC2|C-REGION| [len=317] (mis=0)
umis assigned: [3, 4]
of which 1 are surviving nonsolos
reads assigned: 276
start codons at 52, 57, 113, 200, 346, 350
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.195 = GTCTCGTTCTAAGCCA-1

using 283 reads

====================================================================================

graph has 210 edges initially, 4 edges after simplification

total ucounts = 10
nonsolo ucounts = 3[4, 74, 198]
surviving nonsolo ucounts = 2[74, 198]
ids = [3, 8]

====================================================================================

UMI info for barcode GTCTCGTTCTAAGCCA-1 contig 1 = AGCTCTGGGA...
umi GCCAAATGCG = 192 reads: +412 validated

UMI info for barcode GTCTCGTTCTAAGCCA-1 contig 2 = CTGCTTCAGC...
umi CAAGGACCTT = 65 reads: +391 validated

GOOD CONTIGS

TIG 1[bases=504]
0-80 ==> 0-80 on |164|IGHV3-73|5'UTR| [len=80] (mis=0)
80-427 ==> 0-347 on |165|IGHV3-73|L-REGION+V-REGION| [len=357] (mis=11)
457-492 ==> 11-46 on |54|IGHJ4|J-REGION| [len=46] (mis=0)
junction support: 1 umis using 11 reads
cdr3 = CSAETTRGYLW at 428, score = 7 + 7
umis assigned: [8]
of which 1 are surviving nonsolos
reads assigned: 188
start codons at 80, 236, 321, 360, 389
confident = false

TIG 2[bases=446]
0-48 ==> 3-51 on |320|IGLV1-40|5'UTR| [len=51] (mis=0)
48-388 ==> 0-340 on |321|IGLV1-40|L-REGION+V-REGION| [len=356] (mis=25)
401-439 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=4)
junction support: 1 umis using 10 reads
cdr3 = CQSYDKSLRGAVF at 372, score = 7 + 8
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 64
start codons at 48, 132, 205, 355, 382
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 84.201 = GTCTCGTTCTGTCTCG-1

using 49 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 2[3, 42]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!
sorting bam, mem = 0.05
running samtools sort -l 8G -o /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk084-ucc6a7a72ce/files/contig_bam_sorted.bam /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk084-ucc6a7a72ce/files/contig_bam.bam

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

3.207 seconds used processing barcodes, peak mem = 0.23
