[stdout]

starting vdj_asm_asm, mem = 0.01
available mem = 834.58 GB
/scratch/etanis/software/cellranger-6.1.2/lib/bin/cr_vdj martian assembly main /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk083-ucc6a7a72ce /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk083-ucc6a7a72ce/files /scratch/etanis/ST-118/multi/dimitri-multi/journal/SC_MULTI_CS.SC_MULTI_CORE.MULTI_GEM_WELL_PROCESSOR.VDJ_B_GEM_WELL_PROCESSOR.SC_VDJ_CONTIG_ASSEMBLER.ASSEMBLE_VDJ.fork0.chnk083.ucc6a7a72ce
n50_n50_rpu = 288

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BARCODE 83.1 = GTCGGGTGTGCAGACA-1

using 90 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[89]
surviving nonsolo ucounts = 1[89]
ids = [0]

====================================================================================

UMI info for barcode GTCGGGTGTGCAGACA-1 contig 1 = GCAGCAAGAT...
umi CTCCTTAGCA = 89 reads: +400 validated

GOOD CONTIGS

TIG 1[bases=544]
8-371 ==> 0-363 on |296|IGKV4-1|L-REGION+V-REGION| [len=363] (mis=9)
369-408 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=5)
408-544 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 18 reads
cdr3 = CQHYYSIPPTF at 347, score = 9 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 87
start codons at 8, 77, 330, 450
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 83.7 = GTCGGGTGTTGCTCCT-1

using 3259 reads

====================================================================================

graph has 1515 edges initially, 36 edges after simplification

total ucounts = 259
nonsolo ucounts = 104[2^42, 3^16, 4^8, 5^2, 6^5, 7^2, 8, 9, 10, 11, 13, 14, 68, 69, 71, 73^2, 82, 90, 98, 103^2, 105^2, 111, 125, 129, 141, 142, 148, 156, 191, 202, 213, 223]
surviving nonsolo ucounts = 22[68, 69, 71, 73^2, 82, 98, 103^2, 105^2, 111, 125, 129, 141, 142, 148, 156, 191, 202, 213, 223]
ids = [162, 96, 203, 24, 46, 60, 56, 68, 84, 119, ...]

====================================================================================

UMI info for barcode GTCGGGTGTTGCTCCT-1 contig 1 = AGAGCTCTGG...
umi ACACTAGACT = 216 reads: +288 -1XX +1 -4XX +1 -5XX +2 -1XX +1 -5XX +1 -70 +2 -3X +1 -3X +2 invalidated
umi ACCTGTCGCC = 73 reads: +391 validated
umi ACGGATCACA = 188 reads: +391 validated
umi AGGGTTGTCA = 22 reads: -355 +1 -4X +2 -1X +5 -1X +9 -1XX +4 -1XX +7 invalidated
umi CCAGGACATA = 104 reads: +391 validated
umi CCTCCGACAT = 72 reads: +369 -19 +1 -2 non-validated
umi CGCAGGCGGG = 159 reads: +391 validated
umi GCGTCGCTTC = 223 reads: +391 validated
umi GTACGACCAA = 105 reads: +391 validated
umi GTGTTCTCAC = 108 reads: +312 -1 +68 -1X +9 invalidated
umi TCCGAAGCGC = 71 reads: +391 validated
umi TCGTAACCCT = 141 reads: +391 validated

UMI info for barcode GTCGGGTGTTGCTCCT-1 contig 2 = GGGCTCTGGG...
umi AACGCCCCCC = 109 reads: +401 -20 non-validated
umi AGTGGTTTTA = 72 reads: +287 -4 +75 -55 non-validated
umi ATTGGCGCCT = 74 reads: +421 validated
umi ATTTATCGTT = 147 reads: +421 validated
umi CAATTAGCGG = 104 reads: +399 -1 +9 -1 +11 non-validated
umi CCAATAACGG = 155 reads: -410X +2 -1XX +1 -6XX +1 invalidated
umi GTAAGGTCTT = 69 reads: +319 -9 +56 -37 non-validated
umi GTCATCCACA = 126 reads: +418 -1 +2 non-validated
umi GTTGGTTTCC = 107 reads: +421 validated

GOOD CONTIGS

TIG 1[bases=571]
0-44 ==> 8-52 on |282|IGKV3-20|5'UTR| [len=52] (mis=0)
44-392 ==> 0-348 on |283|IGKV3-20|L-REGION+V-REGION| [len=348] (mis=1)
396-435 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=0)
435-571 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 8 umis using 133 reads
cdr3 = CQQYGSSPPMCSF at 368, score = 9 + 7
umis assigned: [16, 24, 29, 43, 84, 96, 98, 139, 164, 172] and 2 others
of which 11 are surviving nonsolos
reads assigned: 1454
start codons at 44, 252, 378, 395, 477
confident = true

TIG 2[bases=572]
0-80 ==> 0-80 on |125|IGHV3-33|5'UTR| [len=80] (mis=2)
80-431 ==> 0-351 on |126|IGHV3-33|L-REGION+V-REGION| [len=351] (mis=0)
431-458 ==> 0-27 on |18|IGHD3-10|D-REGION| [len=27] (mis=2)
453-501 ==> 0-48 on |55|IGHJ4|J-REGION| [len=48] (mis=4)
501-572 ==> 0-71 on |60|IGHM|C-REGION| [len=1358] (mis=0)
junction support: 5 umis using 46 reads
cdr3 = CARELLWFGELFFDYW at 422, score = 9 + 7
umis assigned: [7, 46, 60, 61, 68, 82, 162, 169, 177]
of which 9 are surviving nonsolos
reads assigned: 954
start codons at 80, 231, 236, 289, 294, 297, 315, 383, 439
confident = true
now this is a cell
paired!

GAGGACACGGCTGTGTATTACTGTGCGAGAGAATTACTATGGTTCGGGGAGTTATTTTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG <==> AGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATGGTAGCTCACCCCCCATGTGCAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAAC

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 83.8 = GTCGGGTGTTGGTGGA-1

using 120 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[119]
surviving nonsolo ucounts = 1[119]
ids = [1]

====================================================================================

UMI info for barcode GTCGGGTGTTGGTGGA-1 contig 1 = GGGAGGAATC...
umi GAGAACGGTG = 121 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=554]
30-381 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
380-418 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
418-554 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 17 reads
cdr3 = CLQYSSSPWTF at 357, score = 9 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 119
start codons at 30, 36, 105, 241, 460
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 83.9 = GTCGGGTGTTGTGGAG-1

using 133 reads

====================================================================================

graph has 64 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 2[2, 128]
surviving nonsolo ucounts = 1[128]
ids = [2]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 83.11 = GTCGGGTGTTTGACTG-1

using 154 reads

====================================================================================

graph has 66 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[3, 150]
surviving nonsolo ucounts = 1[150]
ids = [0]

====================================================================================

UMI info for barcode GTCGGGTGTTTGACTG-1 contig 1 = GGGGGCTGGG...
umi ACGCGCTCGT = 142 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=465]
45-399 ==> 0-354 on |341|IGLV2-18|L-REGION+V-REGION| [len=354] (mis=6)
395-433 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=0)
433-465 ==> 0-32 on |306|IGLC2|C-REGION| [len=317] (mis=1)
junction support: 1 umis using 21 reads
cdr3 = CSSYTTSTTWVF at 369, score = 8 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 138
start codons at 45, 246, 253
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 83.14 = GTCGGGTTCAACGCTA-1

using 143 reads

====================================================================================

graph has 48 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 1[141]
surviving nonsolo ucounts = 1[141]
ids = [1]

====================================================================================

UMI info for barcode GTCGGGTTCAACGCTA-1 contig 1 = AAACCACACC...
umi CCACCGGTTG = 122 reads: +436 validated

GOOD CONTIGS

TIG 1[bases=495]
0-48 ==> 11-59 on |64|IGHV1-18|5'UTR| [len=59] (mis=1)
48-401 ==> 0-353 on |65|IGHV1-18|L-REGION+V-REGION| [len=353] (mis=22)
450-484 ==> 12-46 on |54|IGHJ4|J-REGION| [len=46] (mis=0)
junction support: 1 umis using 10 reads
cdr3 = CARDKHFRDPYYHYSGALDHW at 390, score = 7 + 7
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 121
start codons at 48, 246, 251, 268, 312, 345
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 83.26 = GTCGGGTTCCGAATGT-1

using 859 reads

====================================================================================

graph has 1108 edges initially, 8 edges after simplification

total ucounts = 383
nonsolo ucounts = 137[2^80, 3^27, 4^11, 5^7, 6^6, 7^2, 8, 9, 14, 212]
surviving nonsolo ucounts = 1[212]
ids = [47]

====================================================================================

UMI info for barcode GTCGGGTTCCGAATGT-1 contig 1 = GAGAGGGAAC...
umi ACTCCCAACC = 213 reads: +298 -2XX +1 -3XX +1 -3XX +77 invalidated

GOOD CONTIGS

TIG 1[bases=532]
11-359 ==> 0-348 on |283|IGKV3-20|L-REGION+V-REGION| [len=348] (mis=0)
359-396 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=0)
396-532 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 0 umis using 0 reads
cdr3 = CQQYGSSPLTF at 335, score = 9 + 9
umis assigned: [47]
of which 1 are surviving nonsolos
reads assigned: 206
start codons at 11, 219, 345, 438
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 83.35 = GTCGGGTTCGTAGATC-1

using 3405 reads

====================================================================================

graph has 1951 edges initially, 42 edges after simplification

total ucounts = 578
nonsolo ucounts = 230[2^102, 3^49, 4^17, 5^15, 6^7, 7^8, 8^5, 9^2, 10, 11, 13^2, 14, 19, 24, 45, 62, 63, 75, 81, 88, 102, 112, 127, 134, 140, 144, 149, 166, 170, 199, 208, 237]
surviving nonsolo ucounts = 15[63, 75, 81, 88, 102, 112, 127, 134, 140, 144, 149, 166, 170, 199, 208]
ids = [380, 108, 389, 47, 114, 147, 474, 341, 160, 129, ...]

====================================================================================

UMI info for barcode GTCGGGTTCGTAGATC-1 contig 1 = AGTCCCAGTC...
umi ACATTCCGAG = 87 reads: -2 +386 non-validated
umi AGGCTCCCTC = 77 reads: +299 -1XX +88 invalidated
umi AGTCATGGTA = 100 reads: +388 validated
umi ATAGCCCCGG = 146 reads: +388 validated
umi ATATGCCGCC = 105 reads: -351 +2 -4X +2 -1XX +5 -1XX +9 -1XX +4 -1XX +7 invalidated
umi ATGAACCAGC = 114 reads: +388 validated
umi ATTGTTTCCG = 144 reads: +388 validated
umi CCACGCGCAC = 174 reads: +388 validated
umi CGTTGGAGGT = 149 reads: +388 validated
umi GCCCGTCTAC = 134 reads: +388 validated
umi GGTCATCTGT = 61 reads: +388 validated
umi GGTTATGATG = 81 reads: +388 validated
umi TCCTCTGTAC = 128 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=544]
0-20 ==> 38-58 on |238|IGKV1-9|5'UTR| [len=58] (mis=0)
20-371 ==> 0-351 on |239|IGKV1-9|L-REGION+V-REGION| [len=351] (mis=8)
369-408 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=1)
408-544 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 11 umis using 186 reads
cdr3 = CQQVNSYPCSF at 347, score = 9 + 7
umis assigned: [47, 108, 114, 129, 134, 147, 160, 211, 277, 341] and 3 others
of which 13 are surviving nonsolos
reads assigned: 1469
start codons at 20, 26, 82, 231, 450
confident = true
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 83.38 = GTCGGGTTCTATGTGG-1

using 164 reads

====================================================================================

graph has 94 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 4[2^2, 3, 157]
surviving nonsolo ucounts = 1[157]
ids = [1]

====================================================================================

UMI info for barcode GTCGGGTTCTATGTGG-1 contig 1 = AGGAGTCAGA...
umi CAGATGATGA = 159 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=551]
0-27 ==> 154-181 on |232|IGKV1-5|5'UTR| [len=181] (mis=1)
27-378 ==> 0-351 on |233|IGKV1-5|L-REGION+V-REGION| [len=351] (mis=10)
377-415 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=2)
415-551 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 28 reads
cdr3 = CQQYDSFSWTF at 354, score = 8 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 157
start codons at 27, 33, 89, 102, 238, 241, 334, 364, 457
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 83.44 = GTCGGGTTCTTGTATC-1

using 728 reads

====================================================================================

graph has 1214 edges initially, 6 edges after simplification

total ucounts = 426
nonsolo ucounts = 164[2^99, 3^31, 4^17, 5^7, 6^7, 8, 10, 12]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 83.54 = GTCGTAAAGATGCCTT-1

using 261 reads

====================================================================================

graph has 172 edges initially, 6 edges after simplification

total ucounts = 22
nonsolo ucounts = 17[2^4, 3^3, 4^4, 5^2, 6, 15, 90, 102]
surviving nonsolo ucounts = 2[90, 102]
ids = [13, 5]

====================================================================================

UMI info for barcode GTCGTAAAGATGCCTT-1 contig 1 = CCACCATGGC...
umi CTTTTTGTCA = 79 reads: +385 validated

GOOD CONTIGS

TIG 1[bases=426]
5-346 ==> 0-341 on |337|IGLV2-11|L-REGION+V-REGION| [len=358] (mis=15)
352-390 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=0)
390-426 ==> 0-36 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 16 reads
cdr3 = CSSYAGASWVF at 329, score = 7 + 8
umis assigned: [13]
of which 1 are surviving nonsolos
reads assigned: 79
start codons at 5, 156, 162, 213, 216, 312, 339
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 83.62 = GTCGTAAAGGCGCTCT-1

using 292 reads

====================================================================================

graph has 162 edges initially, 10 edges after simplification

total ucounts = 7
nonsolo ucounts = 7[3, 4^2, 5, 85, 91, 100]
surviving nonsolo ucounts = 4[4, 85, 91, 100]
ids = [4, 6, 0, 3]

====================================================================================

UMI info for barcode GTCGTAAAGGCGCTCT-1 contig 1 = GAATCAGTCC...
umi ACGTATCTCG = 95 reads: +388 validated
umi GTACACCTTG = 101 reads: -2 +20 -1X +18 -1X +6 -1X +3 -1X +3 -302X +1 -17X +1 -5XX +2 -2XX +1 -1XX invalidated
umi TGATGACTTT = 4 reads: +22 -1X +18 -1X +6 -1X +3 -1X +3 -332 invalidated

GOOD CONTIGS

TIG 1[bases=549]
0-25 ==> 2-27 on |222|IGKV1-17|5'UTR| [len=27] (mis=0)
25-376 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=20)
375-413 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
413-549 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 13 reads
cdr3 = CLQYSSSPWTF at 352, score = 9 + 8
umis assigned: [0, 3, 4]
of which 3 are surviving nonsolos
reads assigned: 193
start codons at 25, 31, 100, 236, 455
confident = true

REJECT CONTIGS

TIG 1[bases=425]
3-179 ==> 177-353 on |323|IGLV1-44|L-REGION+V-REGION| [len=353] (mis=8)
176-214 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=3)
214-425 ==> 0-211 on |306|IGLC2|C-REGION| [len=317] (mis=0)
cdr3 = CASWDDSLRGRVF at 147, score = 8 + 8
umis assigned: [6]
of which 1 are surviving nonsolos
reads assigned: 79
start codons at 130, 155, 160
confident = false
not full
VJ delta = 22
not full
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 83.64 = GTCGTAAAGGCTAGCA-1

using 5607 reads

====================================================================================

graph has 3400 edges initially, 34 edges after simplification

total ucounts = 792
nonsolo ucounts = 459[2^136, 3^88, 4^64, 5^46, 6^34, 7^17, 8^14, 9^8, 10^6, 11^3, 14, 15, 16, 29, 36, 41, 43, 44, 49, 52, 60, 63, 64, 71, 75^3, 81, 82, 83^2, 85, 90, 92, 93^2, 96, 97^2, 100, 101, 103, 108, 111, 112^4, 119, 135, 138, 149, 246]
surviving nonsolo ucounts = 35[36, 44, 49, 60, 64, 71, 75^3, 81, 82, 83^2, 85, 90, 92, 93^2, 96, 97^2, 100, 101, 103, 108, 111, 112^4, 119, 135, 138, 149, 246]
ids = [114, 152, 506, 558, 488, 94, 344, 425, 523, 10, ...]

====================================================================================

UMI info for barcode GTCGTAAAGGCTAGCA-1 contig 1 = ATGAGCAAAA...
umi AAATTCTTAT = 80 reads: +379 validated
umi ACTCCTTCAT = 71 reads: +379 validated
umi AGATCCACGG = 36 reads: -11 +312 -56 non-validated
umi AGTAGGAGTG = 79 reads: +379 validated
umi ATACTTACCT = 94 reads: +379 validated
umi ATAGTGTATT = 44 reads: +379 validated
umi ATTCTTCCTG = 102 reads: +379 validated
umi CACAAGCCCG = 102 reads: +379 validated
umi CAGATCTGTC = 113 reads: +379 validated
umi CATACTCTCT = 111 reads: +379 validated
umi CCTGTTCTGA = 104 reads: +379 validated
umi CGCGACTTAA = 84 reads: +379 validated
umi CGGAAGTGTG = 76 reads: +379 validated
umi CGGACTTGAG = 143 reads: +229 -1XX +149 invalidated
umi CTAGTTTCGC = 249 reads: +379 validated
umi CTTCTGCCCT = 92 reads: +379 validated
umi CTTGGGGCCT = 75 reads: +379 validated
umi GACATTTCGG = 82 reads: +379 validated
umi GACTTGACAT = 96 reads: +379 validated
umi GGACAACTTT = 108 reads: +379 validated
umi GGACGTACAG = 69 reads: +10 -3X +1 -5X +1 -1X +2 -1XX +351 -2X +2 invalidated
umi GGTAAACGGT = 50 reads: +379 validated
umi GTAGATTTGG = 73 reads: +379 validated
umi GTCTTTATCT = 111 reads: +379 validated
umi GTTGCTGTGG = 61 reads: +3 -3 +373 non-validated
umi TATATAGGTA = 97 reads: +379 validated
umi TCACACCCCG = 98 reads: +270 -1X +3 -105X invalidated
umi TCCTGTCAAT = 83 reads: +379 validated
umi TCTCGGCAGG = 90 reads: +379 validated
umi TCTGTCCCGC = 116 reads: +269 -3X +107 invalidated
umi TGCACGTTCT = 124 reads: +379 validated
umi TTATTGCATT = 107 reads: +379 validated
umi TTCTATTATG = 95 reads: +379 validated
umi TTTACACCTC = 161 reads: +86 -11XX +282 invalidated
umi TTTCGCCTCT = 135 reads: +379 validated

GOOD CONTIGS

TIG 1[bases=546]
0-31 ==> 33-64 on |299|IGKV6-21|5'UTR| [len=64] (mis=0)
31-373 ==> 0-342 on |300|IGKV6-21|L-REGION+V-REGION| [len=342] (mis=7)
371-410 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=3)
410-546 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 32 umis using 529 reads
cdr3 = CHQSSRLPYTF at 349, score = 9 + 8
umis assigned: [10, 94, 114, 134, 151, 152, 183, 206, 221, 233] and 25 others
of which 35 are surviving nonsolos
reads assigned: 3344
start codons at 0, 31, 236, 314, 332, 452
confident = true
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 83.65 = GTCGTAAAGGGCATGT-1

using 253 reads

====================================================================================

graph has 94 edges initially, 6 edges after simplification

total ucounts = 5
nonsolo ucounts = 5[2^2, 31, 90, 128]
surviving nonsolo ucounts = 3[31, 90, 128]
ids = [0, 1, 4]

====================================================================================

UMI info for barcode GTCGTAAAGGGCATGT-1 contig 1 = ATGGACCTCC...
umi CATGCAGTGA = 83 reads: +442 validated

GOOD CONTIGS

TIG 1[bases=467]
0-369 ==> 0-369 on |184|IGHV4-34|L-REGION+V-REGION| [len=369] (mis=5)
392-442 ==> 0-50 on |53|IGHJ3|J-REGION| [len=50] (mis=2)
442-467 ==> 0-25 on |60|IGHM|C-REGION| [len=1358] (mis=0)
junction support: 1 umis using 11 reads
cdr3 = CVVDSGYEFPSAAFDIW at 360, score = 9 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 81
start codons at 0, 21, 65, 151, 423
confident = false

REJECT CONTIGS

TIG 1[bases=346]
0-30 ==> 16-46 on |389|IGLV8-61|5'UTR| [len=46] (mis=0)
30-320 ==> 0-290 on |390|IGLV8-61|L-REGION+V-REGION| [len=368] (mis=3)
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 127
start codons at 30, 45, 54, 57, 82
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 83.66 = GTCGTAAAGGGTGTTG-1

using 306 reads

====================================================================================

graph has 413 edges initially, 18 edges after simplification

total ucounts = 144
nonsolo ucounts = 66[2^27, 3^23, 4^5, 5^2, 6^3, 7^2, 9, 10, 11, 13]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 83.71 = GTCGTAAAGTCAATAG-1

using 89 reads

====================================================================================

graph has 92 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 3[3, 4, 78]
surviving nonsolo ucounts = 1[78]
ids = [6]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 83.77 = GTCGTAAAGTGGAGAA-1

using 104 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[4, 98]
surviving nonsolo ucounts = 1[98]
ids = [1]

====================================================================================

REJECT CONTIGS

TIG 1[bases=300]
1-73 ==> 5665-5737 on segment before IGKV1OR2-6 exon 1 [len=5879] (mis=1)
25-115 ==> 0-90 on |229|IGKV1-37|L-REGION+V-REGION| [len=351] (mis=4)
116-165 ==> 90-139 on |229|IGKV1-37|L-REGION+V-REGION| [len=351] (mis=8) [SHIFT!]
164-300 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 96
start codons at 25, 31, 130, 206
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 83.83 = GTCGTAAAGTTCGCGC-1

using 8467 reads

====================================================================================

graph has 3490 edges initially, 32 edges after simplification

total ucounts = 530
nonsolo ucounts = 355[2^73, 3^43, 4^48, 5^39, 6^20, 7^17, 8^9, 9^10, 10^2, 11^2, 12, 14, 15, 17, 24, 29^2, 30, 31^2, 34, 36, 43, 44^2, 45, 48, 49, 50, 53^2, 54, 56^2, 58^2, 59^2, 61, 62, 64, 66, 67^2, 68, 70^2, 71, 73^2, 74^2, 75, 77^2, 79^3, 80, 82^4, 83, 85^3, 86, 88^4, 91, 93^2, 94, 95, 96, 98, 100, 101^4, 102, 103, 105, 108, 109^2, 113, 115, 118^2, 120, 123, 127^2, 153^2, 167, 212]
surviving nonsolo ucounts = 85[30, 31^2, 34, 36, 43, 44^2, 45, 48, 49, 50, 53^2, 54, 56^2, 58^2, 59^2, 61, 62, 64, 66, 67^2, 68, 70^2, 71, 73^2, 74^2, 75, 77^2, 79^3, 80, 82^4, 83, 85^3, 86, 88^4, 91, 93^2, 94, 95, 96, 98, 100, 101^4, 102, 103, 105, 108, 109^2, 113, 115, 118^2, 120, 123, 127^2, 153^2, 167, 212]
ids = [26, 13, 167, 91, 318, 191, 145, 381, 386, 55, ...]

====================================================================================

UMI info for barcode GTCGTAAAGTTCGCGC-1 contig 1 = AGGAGTCAGA...
umi AAAATGGGGC = 85 reads: +388 validated
umi AAAGGGGGCT = 81 reads: +388 validated
umi AACGGTGGGG = 32 reads: -1 +335 -1 +51 non-validated
umi AAGACTCACC = 132 reads: -357X +1 -5XX +1 -6XX +1 -3XX +1 -5XX +1 -1XX +1 -2XX +2 -1XX invalidated
umi AAGGTTCGGA = 31 reads: -11 +334 -1 +16 -26 non-validated
umi AATCCCGCCG = 73 reads: +388 validated
umi ACCCGCTCCT = 156 reads: +388 validated
umi ACGCTATCCT = 50 reads: +40 -16 +332 non-validated
umi ACGTTTTGTG = 60 reads: +17 -7 +364 non-validated
umi ACTGCCCCTA = 83 reads: -10 +378 non-validated
umi AGCTTCCTTT = 35 reads: +47 -3 +257 -1X +72 -8 invalidated
umi AGGATGGCTC = 73 reads: +388 validated
umi AGGGGGTCAT = 72 reads: +17 -23 +348 non-validated
umi AGTTCAGGCC = 93 reads: +388 validated
umi AGTTCGTTTG = 100 reads: +388 validated
umi AGTTCTTTCA = 104 reads: +388 validated
umi ATATTAGCTA = 86 reads: +388 validated
umi ATCATCCAAT = 78 reads: +388 validated
umi ATCCGGCACT = 154 reads: +388 validated
umi ATCTATCTGT = 116 reads: +388 validated
umi ATTATACTAG = 84 reads: +388 validated
umi ATTCCCTCCG = 79 reads: +388 validated
umi ATTCTAGACA = 78 reads: +388 validated
umi ATTGTCGGGA = 44 reads: +331 -1 +1 -1 +1 -1 +7 -1 +44 non-validated
umi CAATCACGGA = 125 reads: +388 validated
umi CAATCGCTCT = 111 reads: +388 validated
umi CACGCAGGTG = 31 reads: -2 +374 -1 +1 -10 non-validated
umi CATACTACCG = 74 reads: -21 +367 non-validated
umi CATGACCAGC = 106 reads: +56 -2X +1 -1 +328 invalidated
umi CATTACTAAG = 44 reads: -13 +313 -33 +29 non-validated
umi CATTATCCCT = 64 reads: +388 validated
umi CCAACAACGG = 58 reads: +359 -2 +3 -1 +20 -1 +2 non-validated
umi CCAATTCTGA = 124 reads: +388 validated
umi CCCAATCTTC = 54 reads: +388 validated
umi CCCCTGCCTG = 122 reads: +388 validated
umi CCGGGTCGCG = 56 reads: +388 validated
umi CCGGGTCGCT = 69 reads: +388 validated
umi CCGTCTACCT = 102 reads: +388 validated
umi CGCCCACTGG = 90 reads: +386 -1X +1 invalidated
umi CGCTTGGAGT = 52 reads: +36 -1X +1 -1X +2 -2XX +1 -5XX +2 -1XX +293 -43 invalidated
umi CGTTACCGCA = 85 reads: -354X +2 -1XX +2 -1XX +5 -1XX +9 -1XX +4 -1XX +7 invalidated
umi CTATGAAGCG = 71 reads: +388 validated
umi CTCATATCAT = 169 reads: +388 validated
umi CTCTTCACCA = 56 reads: +388 validated
umi CTTAGTGTCC = 101 reads: +388 validated
umi CTTCTATCCG = 118 reads: +388 validated
umi CTTTGCTGCC = 118 reads: -1 +387 non-validated
umi GCACAGGTCG = 72 reads: +388 validated
umi GCACATTGGA = 99 reads: -215X +173 invalidated
umi GCAGGTCGGC = 36 reads: +10 -1 +377 non-validated
umi GCTCGTTGAA = 80 reads: +388 validated
umi GGAAATGCCG = 91 reads: -4 +384 non-validated
umi GGATATCTAG = 74 reads: -2 +351 -6 +29 non-validated
umi GGCAAGGCCT = 58 reads: -2 +3 -1 +2 -1 +2 -1 +1 -1 +4 -1X +369 invalidated
umi GGTCCACCAC = 87 reads: +388 validated
umi GTAAGTATAT = 58 reads: +388 validated
umi GTATATGCAT = 89 reads: +388 validated
umi GTATATTCAT = 82 reads: +388 validated
umi GTCTTGACCT = 67 reads: -4 +349 -31 +4 non-validated
umi GTGCTACCTG = 49 reads: +4 -1 +1 -2 +3 -1 +1 -1 +8 -10 +356 non-validated
umi GTTAATAACT = 109 reads: +388 validated
umi GTTGCCCACT = 106 reads: +388 validated
umi TAAATTTCGC = 112 reads: +388 validated
umi TAACTGGCCA = 59 reads: -17 +356 -1 +2 -1 +11 non-validated
umi TAAGCCGTGG = 43 reads: +388 validated
umi TAATTCGGGC = 46 reads: +388 validated
umi TAGAGTATTA = 85 reads: +386 -1 +1 non-validated
umi TATATATCTG = 64 reads: +388 validated
umi TATATATTTG = 95 reads: +388 validated
umi TCACATTCTC = 95 reads: +388 validated
umi TCAGCTAGCT = 125 reads: +388 validated
umi TCCAAGTCTT = 118 reads: +388 validated
umi TGCTGGTTAC = 94 reads: +388 validated
umi TGGCAGTTCT = 73 reads: +388 validated
umi TGTCAGTCTT = 81 reads: +388 validated
umi TTAAAATAGC = 79 reads: +388 validated
umi TTACGGCATG = 56 reads: +388 validated
umi TTATGCTATC = 90 reads: +388 validated
umi TTCGTAACAG = 105 reads: +388 validated
umi TTCTTTCCGC = 82 reads: +388 validated
umi TTGCCTGCCC = 63 reads: +388 validated
umi TTGGCGTTCG = 66 reads: +388 validated
umi TTTACTGGAC = 89 reads: +388 validated
umi TTTTGAGTAC = 48 reads: +59 -8 +321 non-validated

GOOD CONTIGS

TIG 1[bases=551]
0-27 ==> 154-181 on |232|IGKV1-5|5'UTR| [len=181] (mis=0)
27-378 ==> 0-351 on |233|IGKV1-5|L-REGION+V-REGION| [len=351] (mis=0)
377-415 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=3)
415-551 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 72 umis using 962 reads
cdr3 = CQQYNSYSPTF at 354, score = 8 + 8
umis assigned: [1, 4, 13, 17, 26, 31, 49, 55, 60, 68] and 74 others
of which 84 are surviving nonsolos
reads assigned: 6779
start codons at 27, 33, 89, 102, 334, 457
confident = true

REJECT CONTIGS

TIG 1[bases=443]
1-73 ==> 276-348 on rc of segment before IGHJ5 exon 1 [len=348] (mis=0)
123-280 ==> 0-157 on rc of segment before IGHJ3P exon 1 [len=157] (mis=0)
278-341 ==> 0-63 on |737|IGHJ6|J-REGION| [len=63] (mis=0)
341-443 ==> 0-102 on |6|IGHD|C-REGION| [len=1289] (mis=0)
umis assigned: [148]
of which 1 are surviving nonsolos
reads assigned: 102
start codons at 130, 164, 203, 258, 298, 359, 420
confident = false
did not find CDR3
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 83.84 = GTCGTAACAAACAACA-1

using 82 reads

====================================================================================

graph has 60 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[3, 77]
surviving nonsolo ucounts = 1[77]
ids = [3]

====================================================================================

UMI info for barcode GTCGTAACAAACAACA-1 contig 1 = AGCTGTGGGC...
umi TCTACCCTTA = 71 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=528]
0-40 ==> 0-40 on |356|IGLV3-19|5'UTR| [len=40] (mis=0)
40-377 ==> 0-337 on |357|IGLV3-19|L-REGION+V-REGION| [len=337] (mis=0)
384-422 ==> 0-38 on |309|IGLJ1|J-REGION| [len=38] (mis=0)
422-528 ==> 0-106 on |305|IGLC1|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 17 reads
cdr3 = CNSRDSSGNHYVF at 355, score = 8 + 8
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 69
start codons at 40, 159, 188, 239, 338, 386
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 83.86 = GTCGTAACAAGCTGTT-1

using 50 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 2[2, 44]
surviving nonsolo ucounts = 1[44]
ids = [2]

====================================================================================

UMI info for barcode GTCGTAACAAGCTGTT-1 contig 1 = CCATGGAAGC...
umi CTCTAACGTT = 38 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=390]
2-347 ==> 0-345 on |279|IGKV3-11|L-REGION+V-REGION| [len=345] (mis=21)
351-390 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=3)
junction support: 1 umis using 5 reads
cdr3 = CQQRYNWPREYTF at 323, score = 9 + 8
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 37
start codons at 2, 207
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 83.88 = GTCGTAACAAGTTGTC-1

using 276 reads

====================================================================================

graph has 116 edges initially, 4 edges after simplification

total ucounts = 8
nonsolo ucounts = 4[4, 46, 84, 138]
surviving nonsolo ucounts = 3[46, 84, 138]
ids = [7, 0, 4]

====================================================================================

UMI info for barcode GTCGTAACAAGTTGTC-1 contig 1 = GATCAGGACT...
umi AAATAGCTCG = 77 reads: +397 validated

UMI info for barcode GTCGTAACAAGTTGTC-1 contig 2 = AGCTTCAGCT...
umi GACTCACTTC = 128 reads: +388 validated
umi TTTGTTTACT = 39 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=513]
0-30 ==> 0-30 on |274|IGKV2D-29|5'UTR| [len=30] (mis=0)
30-390 ==> 0-360 on |275|IGKV2D-29|L-REGION+V-REGION| [len=360] (mis=16)
399-427 ==> 11-39 on |214|IGKJ2|J-REGION| [len=39] (mis=1)
427-513 ==> 0-86 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 15 reads
cdr3 = CMQCIQLPWTF at 366, score = 8 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 76
start codons at 30, 63, 99, 199, 250, 349, 369, 374, 469
confident = true

TIG 2[bases=474]
0-47 ==> 67-114 on |322|IGLV1-44|5'UTR| [len=114] (mis=0)
47-400 ==> 0-353 on |323|IGLV1-44|L-REGION+V-REGION| [len=353] (mis=15)
397-435 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=3)
435-474 ==> 0-39 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 2 umis using 27 reads
cdr3 = CASWDDSLRGRVF at 368, score = 8 + 8
umis assigned: [4, 7]
of which 2 are surviving nonsolos
reads assigned: 166
start codons at 47, 201, 351, 376, 381
confident = true
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 83.91 = GTCGTAACACAGTCGC-1

using 213 reads

====================================================================================

graph has 66 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[3, 210]
surviving nonsolo ucounts = 1[210]
ids = [0]

====================================================================================

UMI info for barcode GTCGTAACACAGTCGC-1 contig 1 = GAGCTGCTCA...
umi AATCGGTCCC = 181 reads: +385 validated

GOOD CONTIGS

TIG 1[bases=451]
0-30 ==> 22-52 on |282|IGKV3-20|5'UTR| [len=52] (mis=0)
30-378 ==> 0-348 on |283|IGKV3-20|L-REGION+V-REGION| [len=348] (mis=7)
376-415 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=5)
415-451 ==> 0-36 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 33 reads
cdr3 = CQQYGSSPGTF at 354, score = 9 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 180
start codons at 30, 238, 364
confident = false
NOT paired!

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BARCODE 83.93 = GTCGTAACACATCTTT-1

using 9 reads

====================================================================================

graph has 6 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[9]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!
sorting bam, mem = 0.05
running samtools sort -l 8G -o /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk083-ucc6a7a72ce/files/contig_bam_sorted.bam /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk083-ucc6a7a72ce/files/contig_bam.bam

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5.191 seconds used processing barcodes, peak mem = 0.23
