[stdout]

starting vdj_asm_asm, mem = 0.01
available mem = 834.13 GB
/scratch/etanis/software/cellranger-6.1.2/lib/bin/cr_vdj martian assembly main /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk081-ucc6a7a72ce /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk081-ucc6a7a72ce/files /scratch/etanis/ST-118/multi/dimitri-multi/journal/SC_MULTI_CS.SC_MULTI_CORE.MULTI_GEM_WELL_PROCESSOR.VDJ_B_GEM_WELL_PROCESSOR.SC_VDJ_CONTIG_ASSEMBLER.ASSEMBLE_VDJ.fork0.chnk081.ucc6a7a72ce
n50_n50_rpu = 288

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 81.0 = GTCACGGAGACGCAAC-1

using 29703 reads

====================================================================================

graph has 7230 edges initially, 44 edges after simplification

total ucounts = 540
nonsolo ucounts = 309[2^77, 3^49, 4^26, 5^19, 6^3, 7^11, 8^7, 10^2, 12^2, 32, 33^2, 41, 48, 67, 70, 76, 78, 83, 92, 94, 111, 114, 124^2, 130, 133, 141, 146, 151, 152, 164, 167, 179^2, 192^2, 193, 194, 195, 196, 198, 208, 209, 210, 214, 221, 225, 228, 229, 230, 231, 232, 234, 235, 237, 240, 242^2, 245, 246, 247, 249^2, 250, 251, 254, 255, 257, 259, 262, 266, 275, 277, 281, 282^2, 285, 287^2, 300^2, 301, 306, 309, 310^3, 312, 314^2, 317, 318, 320, 330^2, 333, 334, 335^2, 354, 356, 363, 369, 373, 375, 379^2, 384, 385, 387, 401, 403, 404, 409, 412, 421, 430, 432, 434, 498, 556]
surviving nonsolo ucounts = 103[41, 76, 78, 83, 92, 114, 124, 130, 133, 141, 151, 152, 164, 167, 179^2, 192^2, 193, 194, 195, 196, 198, 208, 209, 210, 214, 221, 225, 228, 229, 230, 231, 232, 234, 235, 237, 240, 242^2, 245, 246, 247, 249^2, 250, 251, 254, 255, 257, 259, 262, 266, 275, 277, 281, 282^2, 285, 287^2, 300^2, 301, 306, 309, 310^3, 312, 314^2, 317, 318, 320, 330^2, 333, 334, 335^2, 354, 356, 363, 369, 373, 375, 379^2, 384, 385, 387, 401, 403, 404, 409, 412, 421, 430, 432, 434, 498, 556]
ids = [323, 309, 264, 253, 503, 403, 319, 377, 530, 302, ...]

====================================================================================

UMI info for barcode GTCACGGAGACGCAAC-1 contig 1 = TGGGGATGCT...
umi AAACCCAACA = 220 reads: +457 validated
umi AAATTGTAGC = 280 reads: +457 validated
umi AACTGCTCTT = 248 reads: +457 validated
umi AACTTTTATG = 200 reads: +444 -1 +3 -1 +3 -5 non-validated
umi AAGCGATACC = 227 reads: +457 validated
umi AATCATTGGC = 228 reads: +457 validated
umi AGCCAGTGAC = 234 reads: +457 validated
umi AGTGTAGTAG = 321 reads: +457 validated
umi ATAGCCAGCA = 236 reads: +457 validated
umi ATATGCAGAG = 240 reads: +86 -1XX +370 invalidated
umi ATCCATTGTT = 180 reads: +457 validated
umi ATGCCTCGGA = 308 reads: +457 validated
umi ATGTCTTGGG = 282 reads: +457 validated
umi ATTGCATCTA = 329 reads: +457 validated
umi ATTTTCTCTA = 328 reads: +455 -1XX +1 invalidated
umi CAAATTAATA = 361 reads: +457 validated
umi CAACTACGAT = 242 reads: +457 validated
umi CAGTTACCAT = 255 reads: +457 validated
umi CATTAACCCT = 243 reads: +457 validated
umi CCAACCTCCG = 299 reads: +457 validated
umi CCGCCCACAG = 250 reads: +455 -2 non-validated
umi CGAAACCCTG = 279 reads: +457 validated
umi CGCGTATATG = 301 reads: +457 validated
umi CGTCATGGAG = 263 reads: +457 validated
umi CTGAACGGCT = 83 reads: +455 -2 non-validated
umi CTGAATTGGC = 288 reads: +457 validated
umi CTTGGGGTGA = 78 reads: +457 validated
umi GAAATGAGCC = 191 reads: +443 -14 non-validated
umi GAATGTACTG = 206 reads: +457 validated
umi GAGAAATGGT = 178 reads: +432 -25 non-validated
umi GATCAAGGCA = 231 reads: -2X +455 invalidated
umi GATTAAACAC = 262 reads: +457 validated
umi GCACGTACCC = 140 reads: +457 validated
umi GCAGGTTTTG = 140 reads: +457 validated
umi GCCATTCCTT = 178 reads: +457 validated
umi GCCTTCTCCT = 75 reads: +358 -14 +56 -29 non-validated
umi GCGCTGGTGC = 306 reads: +457 validated
umi GCGGATATAG = 124 reads: +457 validated
umi GCGTTAGCGT = 290 reads: +457 validated
umi GGCTCCCTCG = 265 reads: +457 validated
umi GGGTAGTCAA = 246 reads: +457 validated
umi GTTCAGAGGA = 131 reads: -13 +444 non-validated
umi GTTGCACTTA = 319 reads: +457 validated
umi TAACTACGTC = 228 reads: +457 validated
umi TACTTGAGCT = 172 reads: +457 validated
umi TAGAGCACAT = 114 reads: +442 -1 +14 non-validated
umi TCCGTGTGCT = 169 reads: +449 -3 +1 -1 +3 non-validated
umi TCGAGAAGGA = 195 reads: +457 validated
umi TCGTGGAGCC = 342 reads: +86 -1XX +370 invalidated
umi TCTAACCAAC = 153 reads: +444 -1 +1 -1 +1 -1 +2 -1 +1 -1 +1 -1 +1 non-validated
umi TCTATAGAGC = 293 reads: +86 -1XX +370 invalidated
umi TCTTACCCGA = 213 reads: +457 validated
umi TGTCGGAGGA = 244 reads: +457 validated
umi TTAAGGATAT = 221 reads: +86 -1XX +370 invalidated
umi TTCAGGCCCA = 246 reads: +457 validated
umi TTCCTCAACA = 313 reads: +457 validated
umi TTTACCTCTA = 108 reads: +457 validated
umi TTTCTCTAAA = 229 reads: +457 validated

GOOD CONTIGS

TIG 1[bases=549]
21-398 ==> 0-377 on |190|IGHV4-39|L-REGION+V-REGION| [len=377] (mis=4)
430-478 ==> 0-48 on |55|IGHJ4|J-REGION| [len=48] (mis=4)
478-549 ==> 0-71 on |60|IGHM|C-REGION| [len=1358] (mis=0)
junction support: 48 umis using 889 reads
cdr3 = CARHPPSYFEVAAAGAFDYW at 387, score = 9 + 7
umis assigned: [8, 16, 32, 35, 36, 44, 84, 94, 105, 110] and 48 others
of which 58 are surviving nonsolos
reads assigned: 13121
start codons at 5, 21, 30, 42, 86
confident = true
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 81.6 = GTCACGGAGATGCCAG-1

using 96 reads

====================================================================================

graph has 66 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 3[5^2, 83]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 81.8 = GTCACGGAGCCTCGTG-1

using 83 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[2, 79]
surviving nonsolo ucounts = 1[79]
ids = [2]

====================================================================================

UMI info for barcode GTCACGGAGCCTCGTG-1 contig 1 = GAGGGAACCA...
umi GCACTCAGAC = 72 reads: +385 validated

GOOD CONTIGS

TIG 1[bases=471]
9-357 ==> 0-348 on |283|IGKV3-20|L-REGION+V-REGION| [len=348] (mis=4)
355-394 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=5)
394-471 ==> 0-77 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 18 reads
cdr3 = CQQYGSSPVTF at 333, score = 9 + 8
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 71
start codons at 9, 217, 220, 343, 436
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 81.10 = GTCACGGAGCTGCGAA-1

using 269 reads

====================================================================================

graph has 118 edges initially, 4 edges after simplification

total ucounts = 7
nonsolo ucounts = 4[4, 6, 7, 249]
surviving nonsolo ucounts = 3[4, 7, 249]
ids = [4, 5, 2]

====================================================================================

UMI info for barcode GTCACGGAGCTGCGAA-1 contig 1 = AGGAGTCAGA...
umi CAATGCATTG = 257 reads: +388 validated
umi CCGTTAATCA = 4 reads: -151 +63 -12 +17 -1 +65 -79 non-validated

GOOD CONTIGS

TIG 1[bases=551]
0-27 ==> 154-181 on |232|IGKV1-5|5'UTR| [len=181] (mis=0)
27-378 ==> 0-351 on |233|IGKV1-5|L-REGION+V-REGION| [len=351] (mis=3)
378-415 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=0)
415-551 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 37 reads
cdr3 = CQQYNSYPLTF at 354, score = 8 + 9
umis assigned: [2, 4]
of which 2 are surviving nonsolos
reads assigned: 252
start codons at 27, 33, 89, 102, 334, 457
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 81.28 = GTCACGGAGTGTCCCG-1

using 488 reads

====================================================================================

graph has 164 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 1[483]
surviving nonsolo ucounts = 1[483]
ids = [5]

====================================================================================

UMI info for barcode GTCACGGAGTGTCCCG-1 contig 1 = GGGAGGAACT...
umi TGTGGAGACA = 493 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=559]
0-35 ==> 62-97 on |280|IGKV3-15|5'UTR| [len=97] (mis=0)
35-380 ==> 0-345 on |281|IGKV3-15|L-REGION+V-REGION| [len=345] (mis=0)
385-423 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=2)
423-559 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 65 reads
cdr3 = CQQYNNWPPRETF at 356, score = 9 + 8
umis assigned: [5]
of which 1 are surviving nonsolos
reads assigned: 480
start codons at 35, 104, 240, 465
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 81.31 = GTCACGGCAAACAACA-1

using 358 reads

====================================================================================

graph has 152 edges initially, 4 edges after simplification

total ucounts = 6
nonsolo ucounts = 2[160, 194]
surviving nonsolo ucounts = 2[160, 194]
ids = [0, 1]

====================================================================================

UMI info for barcode GTCACGGCAAACAACA-1 contig 1 = TGGGGTCACA...
umi CATGCGGGGT = 189 reads: +385 validated

GOOD CONTIGS

TIG 1[bases=529]
0-39 ==> 123-162 on |342|IGLV2-23|5'UTR| [len=162] (mis=0)
39-379 ==> 0-340 on |343|IGLV2-23|L-REGION+V-REGION| [len=340] (mis=15)
386-424 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=4)
424-529 ==> 0-105 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 26 reads
cdr3 = CCSFADSRGVF at 363, score = 7 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 186
start codons at 39, 178, 240, 247
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 81.36 = GTCACGGCAAGCGCTC-1

using 43 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 4[2^2, 4, 34]
surviving nonsolo ucounts = 1[34]
ids = [1]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 81.41 = GTCACGGCACAACTGT-1

using 424 reads

====================================================================================

graph has 172 edges initially, 4 edges after simplification

total ucounts = 6
nonsolo ucounts = 3[5, 61, 355]
surviving nonsolo ucounts = 2[61, 355]
ids = [3, 2]

====================================================================================

UMI info for barcode GTCACGGCACAACTGT-1 contig 1 = GGAGGAACTG...
umi CCGTTTCCCT = 321 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=517]
26-385 ==> 0-359 on |294|IGKV3D-7|L-REGION+V-REGION| [len=359] (mis=2)
381-420 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=4)
420-517 ==> 0-97 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 67 reads
cdr3 = CQQDYNLPYTF at 359, score = 9 + 8
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 313
start codons at 26, 34, 104, 243, 462
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 81.43 = GTCACGGCACAGACTT-1

using 15 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 81.44 = GTCACGGCACAGAGGT-1

using 1411 reads

====================================================================================

graph has 2161 edges initially, 18 edges after simplification

total ucounts = 694
nonsolo ucounts = 313[2^168, 3^63, 4^32, 5^21, 6^7, 7^5, 8^2, 9^5, 10, 11^3, 12^2, 13, 16^2, 22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 81.45 = GTCACGGCACATCTTT-1

using 851 reads

====================================================================================

graph has 240 edges initially, 8 edges after simplification

total ucounts = 7
nonsolo ucounts = 3[115, 362, 370]
surviving nonsolo ucounts = 3[115, 362, 370]
ids = [0, 2, 5]

====================================================================================

UMI info for barcode GTCACGGCACATCTTT-1 contig 1 = GAGCTACAAC...
umi GCATGCCCGC = 370 reads: +400 validated

UMI info for barcode GTCACGGCACATCTTT-1 contig 2 = AGTCTCAGTC...
umi AAAATCCTTA = 115 reads: +388 validated

UMI info for barcode GTCACGGCACATCTTT-1 contig 3 = GGGGATGCTT...
umi ACGTACGATA = 363 reads: +448 validated

GOOD CONTIGS

TIG 1[bases=566]
0-30 ==> 145-175 on |295|IGKV4-1|5'UTR| [len=175] (mis=1)
30-393 ==> 0-363 on |296|IGKV4-1|L-REGION+V-REGION| [len=363] (mis=25)
392-430 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=2)
430-566 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 53 reads
cdr3 = CQQYYTIPWTF at 369, score = 9 + 8
umis assigned: [5]
of which 1 are surviving nonsolos
reads assigned: 365
start codons at 30, 99, 195, 352, 472
confident = false

TIG 2[bases=544]
0-20 ==> 3-23 on |252|IGKV1D-39|5'UTR| [len=23] (mis=0)
20-373 ==> 0-353 on |253|IGKV1D-39|L-REGION+V-REGION| [len=353] (mis=12)
369-408 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=4)
408-544 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 22 reads
cdr3 = CHESYSSPHTF at 347, score = 9 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 115
start codons at 20, 26, 82, 95, 231, 351, 450
confident = false

TIG 3[bases=539]
20-397 ==> 0-377 on |190|IGHV4-39|L-REGION+V-REGION| [len=377] (mis=3)
418-468 ==> 0-50 on |53|IGHJ3|J-REGION| [len=50] (mis=1)
468-539 ==> 0-71 on |60|IGHM|C-REGION| [len=1358] (mis=0)
junction support: 1 umis using 30 reads
cdr3 = CASFILTGYYSDAFDIW at 386, score = 9 + 8
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 359
start codons at 4, 20, 29, 41, 85, 420, 449
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 81.48 = GTCACGGCACGAAACG-1

using 255 reads

====================================================================================

graph has 62 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 4[3, 5, 7, 237]
surviving nonsolo ucounts = 1[237]
ids = [0]

====================================================================================

UMI info for barcode GTCACGGCACGAAACG-1 contig 1 = GGAATCAGTC...
umi ATTTCTAGTC = 215 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=459]
0-26 ==> 1-27 on |222|IGKV1-17|5'UTR| [len=27] (mis=0)
26-377 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
376-414 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
414-459 ==> 0-45 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 51 reads
cdr3 = CLQYSSSPWTF at 353, score = 9 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 214
start codons at 26, 32, 101, 237
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 81.53 = GTCACGGCACGGTAGA-1

using 353 reads

====================================================================================

graph has 94 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 1[349]
surviving nonsolo ucounts = 1[349]
ids = [2]

====================================================================================

UMI info for barcode GTCACGGCACGGTAGA-1 contig 1 = GTCAGACCCT...
umi CATCGTATGG = 350 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=547]
0-29 ==> 24-53 on |236|IGKV1-8|5'UTR| [len=53] (mis=0)
29-374 ==> 0-345 on |237|IGKV1-8|L-REGION+V-REGION| [len=345] (mis=2)
373-411 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
411-547 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 49 reads
cdr3 = CQQYYSYTWTF at 350, score = 9 + 8
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 346
start codons at 29, 85, 98, 234, 453
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 81.62 = GTCACGGCAGGAATGC-1

using 214 reads

====================================================================================

graph has 118 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 2[6, 205]
surviving nonsolo ucounts = 1[205]
ids = [3]

====================================================================================

UMI info for barcode GTCACGGCAGGAATGC-1 contig 1 = ATCAGTCCCA...
umi CCCCAGCGGA = 176 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=442]
0-23 ==> 4-27 on |222|IGKV1-17|5'UTR| [len=27] (mis=0)
23-374 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
373-411 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
411-442 ==> 0-31 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 32 reads
cdr3 = CLQYSSSPWTF at 350, score = 9 + 8
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 172
start codons at 23, 29, 98, 234
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 81.67 = GTCACGGCATATGAGA-1

using 209 reads

====================================================================================

graph has 82 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 3[3, 7, 192]
surviving nonsolo ucounts = 1[192]
ids = [6]

====================================================================================

UMI info for barcode GTCACGGCATATGAGA-1 contig 1 = GCTGGGGTCT...
umi CTTCTATTCT = 185 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=530]
0-41 ==> 122-163 on |346|IGLV2-8|5'UTR| [len=163] (mis=0)
41-395 ==> 0-354 on |347|IGLV2-8|L-REGION+V-REGION| [len=354] (mis=9)
391-429 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=0)
429-530 ==> 0-101 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 26 reads
cdr3 = CSSYAGSNLVVF at 365, score = 8 + 8
umis assigned: [6]
of which 1 are surviving nonsolos
reads assigned: 184
start codons at 41, 198, 242, 249, 348, 375
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 81.75 = GTCACGGCATGGTAGG-1

using 523 reads

====================================================================================

graph has 210 edges initially, 4 edges after simplification

total ucounts = 7
nonsolo ucounts = 4[2, 4, 197, 317]
surviving nonsolo ucounts = 2[197, 317]
ids = [1, 2]

====================================================================================

UMI info for barcode GTCACGGCATGGTAGG-1 contig 1 = GGGACTGATC...
umi CCAAGTACTG = 296 reads: +397 validated

GOOD CONTIGS

TIG 1[bases=486]
0-36 ==> 0-36 on |272|IGKV2D-28|5'UTR| [len=36] (mis=2)
36-396 ==> 0-360 on |273|IGKV2D-28|L-REGION+V-REGION| [len=360] (mis=0)
396-433 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=1)
433-486 ==> 0-53 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 55 reads
cdr3 = CMQALQTPPTF at 372, score = 9 + 8
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 292
start codons at 36, 69, 105, 193, 355, 375, 475
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 81.81 = GTCACGGGTACAGTGG-1

using 408 reads

====================================================================================

graph has 158 edges initially, 6 edges after simplification

total ucounts = 7
nonsolo ucounts = 4[4, 54, 154, 193]
surviving nonsolo ucounts = 3[54, 154, 193]
ids = [0, 6, 1]

====================================================================================

UMI info for barcode GTCACGGGTACAGTGG-1 contig 1 = TTCAGCTGTG...
umi TTATGAAATC = 146 reads: +391 validated

UMI info for barcode GTCACGGGTACAGTGG-1 contig 2 = TTGGGGAGGA...
umi CTTATTAAAG = 183 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=519]
0-44 ==> 7-51 on |320|IGLV1-40|5'UTR| [len=51] (mis=0)
44-400 ==> 0-356 on |321|IGLV1-40|L-REGION+V-REGION| [len=356] (mis=12)
397-435 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=3)
435-519 ==> 0-84 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 28 reads
cdr3 = CQSYDTSLSGSVF at 368, score = 8 + 8
umis assigned: [6]
of which 1 are surviving nonsolos
reads assigned: 142
start codons at 44, 198, 201, 252, 351, 378
confident = false

TIG 2[bases=507]
33-384 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
383-421 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
421-507 ==> 0-86 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 22 reads
cdr3 = CLQYSSSPWTF at 360, score = 9 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 182
start codons at 33, 39, 108, 244, 463
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 81.83 = GTCACGGGTAGGGTAC-1

using 138 reads

====================================================================================

graph has 76 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 2[5, 129]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 81.85 = GTCACGGGTATGAAAC-1

using 176 reads

====================================================================================

graph has 76 edges initially, 2 edges after simplification

total ucounts = 13
nonsolo ucounts = 8[2^5, 3, 4, 154]
surviving nonsolo ucounts = 1[154]
ids = [6]

====================================================================================

UMI info for barcode GTCACGGGTATGAAAC-1 contig 1 = AGTCTGGGCC...
umi GTTTTTTCCT = 136 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=462]
0-40 ==> 211-251 on |358|IGLV3-21|5'UTR| [len=251] (mis=0)
40-373 ==> 0-333 on |359|IGLV3-21|L-REGION+V-REGION| [len=351] (mis=29)
384-422 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=1)
422-462 ==> 0-40 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 26 reads
cdr3 = CQVWDSDGHYVVF at 355, score = 8 + 8
umis assigned: [6]
of which 1 are surviving nonsolos
reads assigned: 135
start codons at 40, 101, 170, 338, 374, 383
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 81.86 = GTCACGGGTCAAACTC-1

using 522 reads

====================================================================================

graph has 209 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 2[233, 284]
surviving nonsolo ucounts = 2[233, 284]
ids = [0, 5]

====================================================================================

UMI info for barcode GTCACGGGTCAAACTC-1 contig 1 = GCTGTGCTGT...
umi ACTGGACAGT = 231 reads: +382 validated
umi TGATATTCTG = 289 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=636]
0-43 ==> 0-43 on |362|IGLV3-25|5'UTR| [len=43] (mis=0)
43-380 ==> 0-337 on |363|IGLV3-25|L-REGION+V-REGION| [len=337] (mis=0)
387-425 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=0)
425-636 ==> 0-211 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 2 umis using 81 reads
cdr3 = CQSADSSGTYVVF at 358, score = 8 + 8
umis assigned: [0, 5]
of which 2 are surviving nonsolos
reads assigned: 509
start codons at 43, 104, 173, 191, 386
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 81.101 = GTCACGGGTGTAACGG-1

using 576 reads

====================================================================================

graph has 846 edges initially, 10 edges after simplification

total ucounts = 268
nonsolo ucounts = 115[2^52, 3^24, 4^13, 5^17, 6, 7, 8, 9^2, 14, 16, 17, 24]
surviving nonsolo ucounts = 1[24]
ids = [206]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 81.116 = GTCACGGGTTGGTTTG-1

using 446 reads

====================================================================================

graph has 326 edges initially, 2 edges after simplification

total ucounts = 97
nonsolo ucounts = 26[2^14, 3^4, 4^2, 6^2, 7, 9, 11, 288]
surviving nonsolo ucounts = 1[288]
ids = [26]

====================================================================================

UMI info for barcode GTCACGGGTTGGTTTG-1 contig 1 = GATCAGGACT...
umi CAGCCTAACT = 272 reads: +397 validated

GOOD CONTIGS

TIG 1[bases=484]
0-30 ==> 0-30 on |264|IGKV2-28|5'UTR| [len=30] (mis=0)
30-390 ==> 0-360 on |265|IGKV2-28|L-REGION+V-REGION| [len=360] (mis=1)
390-427 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=0)
427-484 ==> 0-57 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 31 reads
cdr3 = CMQALQTPLTF at 366, score = 9 + 9
umis assigned: [26]
of which 1 are surviving nonsolos
reads assigned: 268
start codons at 30, 63, 99, 187, 349, 369, 469
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 81.118 = GTCACGGTCAACACAC-1

using 248 reads

====================================================================================

graph has 160 edges initially, 2 edges after simplification

total ucounts = 14
nonsolo ucounts = 8[2^6, 4, 226]
surviving nonsolo ucounts = 1[226]
ids = [11]

====================================================================================

UMI info for barcode GTCACGGTCAACACAC-1 contig 1 = GCTCTGCTTC...
umi TATACGTCCT = 217 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=528]
0-51 ==> 0-51 on |320|IGLV1-40|5'UTR| [len=51] (mis=0)
51-407 ==> 0-356 on |321|IGLV1-40|L-REGION+V-REGION| [len=356] (mis=5)
401-439 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=3)
439-528 ==> 0-89 on |307|IGLC3|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 23 reads
cdr3 = CQSYDSSLREVF at 375, score = 8 + 8
umis assigned: [11]
of which 1 are surviving nonsolos
reads assigned: 213
start codons at 51, 205, 208, 259, 358, 385
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 81.120 = GTCACGGTCAAGATCC-1

using 325 reads

====================================================================================

graph has 102 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 3[2, 7, 311]
surviving nonsolo ucounts = 1[311]
ids = [3]

====================================================================================

UMI info for barcode GTCACGGTCAAGATCC-1 contig 1 = AGGAGTCAGT...
umi CTCTCACTAC = 292 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=473]
0-27 ==> 4-31 on |226|IGKV1-33|5'UTR| [len=31] (mis=0)
27-378 ==> 0-351 on |227|IGKV1-33|L-REGION+V-REGION| [len=351] (mis=1)
378-415 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=0)
415-473 ==> 0-58 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 50 reads
cdr3 = CQQYDNLPLTF at 354, score = 9 + 9
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 287
start codons at 27, 33, 89, 102, 241, 364, 457
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 81.121 = GTCACGGTCAAGGCTT-1

using 373 reads

====================================================================================

graph has 166 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 5[2, 3, 5^2, 353]
surviving nonsolo ucounts = 1[353]
ids = [9]

====================================================================================

UMI info for barcode GTCACGGTCAAGGCTT-1 contig 1 = AGGAGTCAGA...
umi TGACGTGTCG = 355 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=551]
0-27 ==> 2-29 on |234|IGKV1-6|5'UTR| [len=29] (mis=0)
27-378 ==> 0-351 on |235|IGKV1-6|L-REGION+V-REGION| [len=351] (mis=28)
376-415 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=5)
415-551 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 68 reads
cdr3 = CLQDYTYPFSF at 354, score = 9 + 7
umis assigned: [9]
of which 1 are surviving nonsolos
reads assigned: 348
start codons at 27, 33, 89, 102, 184, 187, 457
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 81.126 = GTCACGGTCAGAAATG-1

using 272 reads

====================================================================================

graph has 86 edges initially, 4 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[20, 37, 213]
surviving nonsolo ucounts = 2[37, 213]
ids = [2, 3]

====================================================================================

UMI info for barcode GTCACGGTCAGAAATG-1 contig 1 = GAGGAATCAG...
umi CCGAAATTCT = 215 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=552]
28-379 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
378-416 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
416-552 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 36 reads
cdr3 = CLQYSSSPWTF at 355, score = 9 + 8
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 212
start codons at 28, 34, 103, 239, 458
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 81.129 = GTCACGGTCATAGCAC-1

using 366 reads

====================================================================================

graph has 142 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 3[2, 4, 356]
surviving nonsolo ucounts = 1[356]
ids = [1]

====================================================================================

UMI info for barcode GTCACGGTCATAGCAC-1 contig 1 = GAGCTGCTCA...
umi ACGAGTTTTG = 319 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=507]
0-30 ==> 22-52 on |282|IGKV3-20|5'UTR| [len=52] (mis=0)
30-378 ==> 0-348 on |283|IGKV3-20|L-REGION+V-REGION| [len=348] (mis=3)
380-418 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=0)
418-507 ==> 0-89 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 64 reads
cdr3 = CQQYGSSPPWTF at 354, score = 9 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 315
start codons at 30, 238, 364, 460
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 81.135 = GTCACGGTCCAGTATG-1

using 71 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 1[69]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 81.143 = GTCACGGTCCTTTACA-1

using 40952 reads

====================================================================================

graph has 12764 edges initially, 134 edges after simplification

total ucounts = 1630
nonsolo ucounts = 747[2^264, 3^133, 4^68, 5^35, 6^22, 7^19, 8^10, 9^4, 10^5, 11^7, 12^3, 13, 14^2, 15, 17^2, 19^2, 22, 23, 31, 32, 37, 40, 42, 47, 48, 50^2, 58, 70, 73, 75, 77, 78, 79, 80, 84, 85, 90, 91, 92, 96, 106, 108, 110, 111^2, 115, 121^2, 124^3, 126, 128, 136, 139^2, 147, 149, 154, 163, 167^2, 170, 171^2, 172^2, 173^2, 179, 180, 186, 187, 188, 190, 192, 194, 196, 198, 199, 200, 203, 207^2, 208, 209, 211, 212, 215^2, 216, 218, 219^2, 220^2, 221^2, 224^3, 226^2, 227, 228, 229, 231, 233, 235, 237, 238, 239^3, 242, 243^2, 244^3, 248, 250, 251, 252, 253^2, 256, 257, 258, 259, 260^2, 261^2, 264, 265^2, 269, 271^2, 276, 277, 278, 279, 280, 281, 282, 284^2, 286, 289, 290, 293, 296, 297, 300, 303, 304^2, 314, 315, 325, 328^2, 333, 342, 346, 348^2, 354, 357, 359^2, 375, 405, 410, 413, 455, 502^2, 632, 676, 723, 775]
surviving nonsolo ucounts = 153[22, 32, 58, 70, 73, 75, 79, 80, 84, 85, 90, 91, 92, 96, 106, 110, 111, 115, 121^2, 124^2, 126, 128, 136, 139^2, 147, 149, 154, 163, 167^2, 170, 171, 172^2, 173^2, 179, 180, 186, 187, 188, 190, 192, 194, 196, 198, 199, 200, 203, 207^2, 208, 209, 211, 212, 215^2, 216, 218, 219^2, 220^2, 221^2, 224^3, 226^2, 227, 228, 229, 231, 233, 235, 237, 238, 239^3, 242, 243^2, 244^3, 248, 250, 251, 252, 253^2, 256, 257, 258, 259, 260^2, 261^2, 264, 265^2, 269, 271^2, 276, 277, 278, 279, 280, 281, 282, 284^2, 286, 289, 290, 293, 296, 297, 300, 303, 304^2, 314, 315, 325, 328^2, 333, 342, 346, 348^2, 354, 357, 359^2, 375, 405, 410, 455, 502^2, 632, 676, 723, 775]
ids = [1377, 3, 366, 1367, 706, 263, 1216, 270, 947, 406, ...]

====================================================================================

UMI info for barcode GTCACGGTCCTTTACA-1 contig 1 = TGGGGATCAC...
umi AAACGCGGTG = 107 reads: +380 -1 +1 -1 +26 non-validated
umi AATAGCTCCT = 279 reads: +409 validated
umi AATTCAAACC = 332 reads: +409 validated
umi AGAGTGGTCG = 356 reads: +409 validated
umi ATAAGTCACA = 226 reads: +409 validated
umi ATATAATACT = 253 reads: +409 validated
umi ATCACCTGCA = 77 reads: +375 -3 +2 -1 +28 non-validated
umi ATCCTCCTCG = 71 reads: -371X +1 -2X +9 -1X +2 -1X +3 -1X +9 -1 +8 invalidated
umi ATCTGGACGG = 243 reads: +409 validated
umi ATTCCATCGG = 179 reads: -364X +2 -5X +1 -2XX +17 -1XX +17 invalidated
umi ATTCCTGGTC = 204 reads: +384 -1 +24 non-validated
umi ATTGATGCCT = 297 reads: +409 validated
umi ATTTATTCGC = 368 reads: +338 -1XX +1 -4XX +65 invalidated
umi ATTTCTCCTG = 329 reads: +409 validated
umi CAAATATCAG = 56 reads: +377 -16 +16 non-validated
umi CAACGTGCAC = 224 reads: +409 validated
umi CACGGCCTCA = 182 reads: +220 -1XX +188 invalidated
umi CAGTCTCGCA = 146 reads: +409 validated
umi CCAAGTCGCG = 138 reads: +409 validated
umi CCATATATGG = 263 reads: -339 +2 -1X +2 -1XX +1 -8XX +1 -5XX +1 -10XX +20 -1XX +17 invalidated
umi CCCACCCCAT = 284 reads: +409 validated
umi CCCGATACTT = 288 reads: +409 validated
umi CCCTCACTCT = 330 reads: +409 validated
umi CCGGGATGGC = 178 reads: +409 validated
umi CCTCACGCCC = 267 reads: +409 validated
umi CCTGCCATAG = 198 reads: +220 -1XX +188 invalidated
umi CCTTTCACTT = 347 reads: +409 validated
umi CGCAGCGGGA = 267 reads: +409 validated
umi CGTGTTGCGT = 73 reads: +409 validated
umi CTACCGCCCG = 347 reads: +409 validated
umi CTATGACCTT = 316 reads: +409 validated
umi CTGATCCACG = 197 reads: +148 -1XX +1 -1X +258 invalidated
umi CTGATTAGGC = 162 reads: +409 validated
umi CTGCACGCAT = 223 reads: +409 validated
umi CTTATGCCCG = 170 reads: +409 validated
umi CTTGCGTGCC = 374 reads: +192 -1XX +1 -4XX +1 -3XX +1 -2X +2 -1XX +1 -1XX +199 invalidated
umi GACATTCCGT = 239 reads: +409 validated
umi GCCCTTTCTC = 170 reads: +409 validated
umi GCTATTCCTC = 275 reads: +220 -1XX +188 invalidated
umi GCTCACAGCC = 234 reads: +409 validated
umi GTATCTTAAG = 220 reads: +409 validated
umi GTGACTCTCC = 123 reads: +409 validated
umi GTTCCTTCAG = 282 reads: +406 -1 +1 -1 non-validated
umi TAAGCGCATC = 283 reads: +409 validated
umi TACGCCATAG = 349 reads: +409 validated
umi TAGCCTACGG = 81 reads: +409 validated
umi TAGGATCTCC = 201 reads: +409 validated
umi TCAACCGTGC = 217 reads: +409 validated
umi TCACTTGAGT = 141 reads: +409 validated
umi TCCCGGTGCG = 208 reads: +409 validated
umi TCCTAACGAT = 213 reads: +409 validated
umi TCTATCACCA = 197 reads: +409 validated
umi TCTCAGCCCT = 257 reads: +409 validated
umi TCTCCGCTCT = 518 reads: +217 -2XX +1 -1XX +2 -8XX +1 -1XX +2 -85X +2 -1XX +1 -6XX +1 -4XX +74 invalidated
umi TCTTGTTCTT = 111 reads: +374 -10 +25 non-validated
umi TGACTCACGA = 247 reads: +409 validated
umi TGGTGCCGGC = 115 reads: +380 -3 +3 -1 +1 -1 +1 -19 non-validated
umi TGTAGGTGTC = 151 reads: +409 validated
umi TTACCTTCGC = 218 reads: +409 validated
umi TTCTACGTCA = 205 reads: +400 -1 +2 -1 +3 -1 +1 non-validated
umi TTCTCTTCCT = 91 reads: +409 validated
umi TTGTGTGGCA = 125 reads: +397 -12 non-validated
umi TTTGTCGCAC = 91 reads: +355 -1 +53 non-validated

UMI info for barcode GTCACGGTCCTTTACA-1 contig 2 = GAGCTACAAC...
umi AAATGACCGA = 346 reads: +400 validated
umi ACGGGGTGAT = 414 reads: -148 +1 -3X +248 invalidated
umi ACTGGTACCA = 300 reads: +400 validated
umi AGAGCGCTCA = 268 reads: +350 -1XX +1 -2XX +1 -1 +44 invalidated
umi AGCCCGACGG = 316 reads: +400 validated
umi ATAAGATATC = 239 reads: +400 validated
umi ATCACGCTAT = 178 reads: +400 validated
umi ATCGCTGGGC = 171 reads: +400 validated
umi ATCTTTTTCT = 262 reads: +350 -1XX +1 -2XX +1 -1XX +1 -4XX +1 -1 +37 invalidated
umi ATTCGATTGA = 391 reads: -365X +3 -1XX +2 -1XX +5 -1XX +5 -4XX +8 -1XX +4 invalidated
umi ATTTTGGTAG = 224 reads: +400 validated
umi CACCTAGGGG = 174 reads: +400 validated
umi CAGTAGTCAG = 226 reads: +400 validated
umi CCAGCGAGCC = 125 reads: +400 validated
umi CCCGTTCACC = 135 reads: +400 validated
umi CCGTATTCCT = 380 reads: +392 -1XX +7 invalidated
umi CCTCTACTGG = 280 reads: +400 validated
umi CGAGTTCTTT = 241 reads: +400 validated
umi CGCTTCTCCT = 232 reads: +400 validated
umi CTCCTCTCGA = 271 reads: +400 validated
umi CTGTTTTACC = 173 reads: +400 validated
umi CTTGCCTTCC = 262 reads: +400 validated
umi CTTTACACAA = 613 reads: -332 +1 -13XX +1 -5XX +1 -3XX +1 -2XX +1 -5XX +2 -1XX +32 invalidated
umi GACCGCCCTC = 173 reads: +400 validated
umi GCACCCTCCT = 307 reads: +400 validated
umi GCAGTTACTA = 260 reads: +400 validated
umi GCCCCCTGGT = 78 reads: -281X +5 -3XX +1 -1XX +1 -4XX +1 -2XX +4 -2XX +87 -1XX +7 invalidated
umi GCTCCGCCGA = 121 reads: +400 validated
umi GCTGCTTCGC = 291 reads: +400 validated
umi GGGGTATTTC = 410 reads: +400 validated
umi GTCACTATGT = 241 reads: +400 validated
umi GTCCAACGCT = 298 reads: +20 -4XX +1 -8XX +1 -1XX +1 -8X +1 -1XX +1 -1XX +352 invalidated
umi GTGATTCATG = 252 reads: +400 validated
umi GTGTTAACCA = 217 reads: +400 validated
umi GTTTTAGTGG = 748 reads: +128 -2XX +1 -12XX +1 -7XX +2 -195 +1 -1X +1 -3XX +1 -12XX +1 -10XX +22 invalidated
umi TACTATTCTC = 209 reads: +400 validated
umi TAGTGCTGCC = 305 reads: +400 validated
umi TATGTACCTT = 286 reads: +392 -1XX +7 invalidated
umi TCAGTTGCCC = 205 reads: -318X +82 invalidated
umi TCCAGATCAC = 255 reads: +147 -2XX +1 -2XX +3 -2XX +1 -2XX +1 -3XX +1 -1XX +234 invalidated
umi TCTAAGGGCT = 218 reads: +400 validated
umi TCTCTTCTAC = 167 reads: +400 validated
umi TCTTTCGTTA = 264 reads: +400 validated
umi TGAGTACTCT = 238 reads: +400 validated
umi TGGACGCTGT = 248 reads: +400 validated
umi TGTTCCTACC = 255 reads: +400 validated
umi TTCAATCGCG = 302 reads: +400 validated
umi TTCTGGTCTT = 296 reads: +400 validated
umi TTGGTAAGCA = 251 reads: +400 validated
umi TTTGTAACTA = 222 reads: +400 validated

GOOD CONTIGS

TIG 1[bases=543]
63-416 ==> 0-353 on |736|IGHV1-8|L-REGION+V-REGION| [len=353] (mis=11)
434-472 ==> 8-46 on |54|IGHJ4|J-REGION| [len=46] (mis=1)
472-543 ==> 0-71 on |60|IGHM|C-REGION| [len=1358] (mis=0)
junction support: 51 umis using 846 reads
cdr3 = CATGLLGGPIYW at 405, score = 9 + 7
umis assigned: [4, 47, 62, 173, 222, 241, 263, 270, 274, 330] and 53 others
of which 62 are surviving nonsolos
reads assigned: 13660
start codons at 63, 214, 261, 266, 270, 298, 327, 345, 360
confident = true

TIG 2[bases=566]
0-30 ==> 145-175 on |295|IGKV4-1|5'UTR| [len=175] (mis=0)
30-393 ==> 0-363 on |296|IGKV4-1|L-REGION+V-REGION| [len=363] (mis=12)
391-430 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=5)
430-566 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 42 umis using 1521 reads
cdr3 = CQHYYSIPPTF at 369, score = 9 + 8
umis assigned: [18, 133, 154, 172, 188, 220, 264, 271, 278, 334] and 40 others
of which 50 are surviving nonsolos
reads assigned: 13081
start codons at 30, 99, 352, 472
confident = true

REJECT CONTIGS

TIG 1[bases=695]
113-421 ==> 0-308 on |327|IGLV1-47|L-REGION+V-REGION| [len=351] (mis=18)
446-484 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=5)
484-695 ==> 0-211 on |306|IGLC2|C-REGION| [len=317] (mis=0)
umis assigned: [35, 67, 98, 175, 186, 238, 360, 406, 419, 534] and 25 others
of which 35 are surviving nonsolos
reads assigned: 6381
start codons at 9, 13, 36, 113, 324, 417
confident = false
did not find CDR3

TIG 2[bases=367]
4-228 ==> 1598-1822 on rc of segment before IGHD5-18 exon 1 [len=1822] (mis=3)
246-296 ==> 0-50 on |53|IGHJ3|J-REGION| [len=50] (mis=4)
296-367 ==> 0-71 on |60|IGHM|C-REGION| [len=1358] (mis=0)
umis assigned: [75, 237]
of which 2 are surviving nonsolos
reads assigned: 1015
start codons at 129, 277
confident = false
did not find CDR3
now this is a cell
paired!

AGCCTGAGATCTGAGGACACGGCCGTCTATTATTGTGCGACAGGCCTCCTAGGGGGGCCAATCTACTGGGGCCAGGGAACCCCGGTCACCGTCTCCTCAG <==> ATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCACTATTATTCTATTCCTCCCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAAC

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 81.145 = GTCACGGTCGGCATCG-1

using 236 reads

====================================================================================

graph has 60 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[2^2, 5, 225]
surviving nonsolo ucounts = 1[225]
ids = [1]

====================================================================================

UMI info for barcode GTCACGGTCGGCATCG-1 contig 1 = AGCTTCAGCT...
umi CATGGTTAAC = 219 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=531]
0-47 ==> 67-114 on |322|IGLV1-44|5'UTR| [len=114] (mis=0)
47-400 ==> 0-353 on |323|IGLV1-44|L-REGION+V-REGION| [len=353] (mis=2)
397-435 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=0)
435-531 ==> 0-96 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 35 reads
cdr3 = CAAWDDSLNGWVF at 368, score = 8 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 217
start codons at 47, 351, 376, 381, 393
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 81.153 = GTCACGGTCTAACTTC-1

using 75 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 4[3^3, 62]
surviving nonsolo ucounts = 1[62]
ids = [6]

====================================================================================

UMI info for barcode GTCACGGTCTAACTTC-1 contig 1 = ACCATGGAAG...
umi TCCAAGACAA = 53 reads: +5 -1 +376 non-validated

GOOD CONTIGS

TIG 1[bases=395]
3-348 ==> 0-345 on |281|IGKV3-15|L-REGION+V-REGION| [len=345] (mis=12)
347-385 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=2)
junction support: 1 umis using 12 reads
cdr3 = CLQYYNWPRTF at 324, score = 9 + 8
umis assigned: [6]
of which 1 are surviving nonsolos
reads assigned: 51
start codons at 3, 58, 72, 208
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 81.154 = GTCACGGTCTACCAGA-1

using 17 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 1[12]
surviving nonsolo ucounts = 1[12]
ids = [1]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 81.158 = GTCACGGTCTGATACG-1

using 659 reads

====================================================================================

graph has 168 edges initially, 4 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[114, 543]
surviving nonsolo ucounts = 2[114, 543]
ids = [1, 2]

====================================================================================

UMI info for barcode GTCACGGTCTGATACG-1 contig 1 = GAAGAGCTGC...
umi TCTTGGTCTT = 543 reads: +385 validated

UMI info for barcode GTCACGGTCTGATACG-1 contig 2 = GTGGGCTCAG...
umi TCGGGCTGGC = 110 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=554]
0-33 ==> 19-52 on |282|IGKV3-20|5'UTR| [len=52] (mis=0)
33-381 ==> 0-348 on |283|IGKV3-20|L-REGION+V-REGION| [len=348] (mis=2)
379-418 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=4)
418-554 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 87 reads
cdr3 = CQQYGSSPSTF at 357, score = 9 + 8
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 538
start codons at 33, 241, 367, 460
confident = false

TIG 2[bases=448]
0-36 ==> 4-40 on |356|IGLV3-19|5'UTR| [len=40] (mis=0)
36-373 ==> 0-337 on |357|IGLV3-19|L-REGION+V-REGION| [len=337] (mis=0)
380-418 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=0)
418-448 ==> 0-30 on |306|IGLC2|C-REGION| [len=317] (mis=1)
junction support: 1 umis using 24 reads
cdr3 = CNSRDSSGNHVVF at 351, score = 8 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 108
start codons at 36, 155, 184, 235, 334, 379
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 81.168 = GTCACGGTCTTGAGAC-1

using 263 reads

====================================================================================

graph has 82 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 4[2^3, 250]
surviving nonsolo ucounts = 1[250]
ids = [10]

====================================================================================

UMI info for barcode GTCACGGTCTTGAGAC-1 contig 1 = AGCTCTCAGA...
umi TTGTGAGCTG = 247 reads: +406 validated

GOOD CONTIGS

TIG 1[bases=522]
0-79 ==> 0-79 on |141|IGHV3-48|5'UTR| [len=79] (mis=0)
79-432 ==> 0-353 on |142|IGHV3-48|L-REGION+V-REGION| [len=353] (mis=15)
437-485 ==> 0-48 on |55|IGHJ4|J-REGION| [len=48] (mis=4)
485-522 ==> 0-37 on |40|IGHG1|C-REGION| [len=884] (mis=0)
junction support: 1 umis using 22 reads
cdr3 = CARVGPLFDYW at 421, score = 9 + 6
umis assigned: [10]
of which 1 are surviving nonsolos
reads assigned: 245
start codons at 79, 235, 356, 382
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 81.190 = GTCATTTAGGGAGTAA-1

using 455 reads

====================================================================================

graph has 176 edges initially, 4 edges after simplification

total ucounts = 8
nonsolo ucounts = 7[2, 3, 5, 9^2, 180, 246]
surviving nonsolo ucounts = 2[180, 246]
ids = [1, 4]

====================================================================================

UMI info for barcode GTCATTTAGGGAGTAA-1 contig 1 = AGGAATCAGT...
umi ATACTCTGTA = 179 reads: +388 validated

UMI info for barcode GTCATTTAGGGAGTAA-1 contig 2 = ACCCAAAAAC...
umi CATCATGCGC = 237 reads: +436 validated

GOOD CONTIGS

TIG 1[bases=551]
0-27 ==> 0-27 on |222|IGKV1-17|5'UTR| [len=27] (mis=0)
27-378 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
377-415 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
415-551 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 31 reads
cdr3 = CLQYSSSPWTF at 354, score = 9 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 178
start codons at 27, 33, 102, 238, 457
confident = false

TIG 2[bases=540]
0-54 ==> 5-59 on |64|IGHV1-18|5'UTR| [len=59] (mis=1)
54-407 ==> 0-353 on |65|IGHV1-18|L-REGION+V-REGION| [len=353] (mis=22)
456-490 ==> 12-46 on |54|IGHJ4|J-REGION| [len=46] (mis=0)
490-540 ==> 0-50 on |1|IGHA1|C-REGION| [len=1058] (mis=0)
junction support: 1 umis using 17 reads
cdr3 = CARDKHFRDPYYHYSGALDHW at 396, score = 7 + 7
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 236
start codons at 54, 252, 257, 274, 318, 351
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 81.193 = GTCATTTAGGGTTCCC-1

using 57 reads

====================================================================================

graph has 58 edges initially, 2 edges after simplification

total ucounts = 16
nonsolo ucounts = 9[2^2, 3^2, 5, 6, 8, 9, 12]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 81.199 = GTCATTTAGTCCGGTC-1

using 12 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 3[2, 3, 4]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 81.204 = GTCATTTAGTGTCTCA-1

using 4071 reads

====================================================================================

graph has 2993 edges initially, 46 edges after simplification

total ucounts = 706
nonsolo ucounts = 325[2^127, 3^81, 4^41, 5^28, 6^13, 7^4, 8^4, 9^3, 10, 11^2, 14, 18, 22, 23, 55, 60, 105, 109, 121, 123, 124, 138, 143, 148, 194, 197, 198, 208, 211, 220, 261]
surviving nonsolo ucounts = 17[22, 60, 105, 109, 121, 123, 124, 138, 143, 148, 194, 197, 198, 208, 211, 220, 261]
ids = [479, 577, 480, 127, 370, 298, 436, 411, 504, 290, ...]

====================================================================================

UMI info for barcode GTCATTTAGTGTCTCA-1 contig 1 = AAGGCATTTC...
umi CAGACAGGCA = 166 reads: +430 validated
umi CCGGATTATA = 123 reads: +430 validated
umi GTTCAGCCCT = 134 reads: +418 -12 non-validated

UMI info for barcode GTCATTTAGTGTCTCA-1 contig 2 = GAGCTACAAC...
umi AGGCAGCCCT = 108 reads: +403 validated
umi CCCGTATTAT = 152 reads: +403 validated
umi GCAAAAGGCC = 142 reads: +403 validated
umi GCTAATTGGG = 125 reads: +403 validated
umi GGCAATAGTA = 218 reads: +403 validated
umi GTAATTTCAC = 23 reads: +362 -41 non-validated
umi GTAATTTCGC = 108 reads: +403 validated
umi TAATATGTAC = 261 reads: +403 validated
umi TATCGGTACG = 205 reads: +403 validated
umi TTGGTCTCTC = 203 reads: +403 validated
umi TTGTCCTGTT = 212 reads: +403 validated

GOOD CONTIGS

TIG 1[bases=634]
0-46 ==> 33-79 on |141|IGHV3-48|5'UTR| [len=79] (mis=0)
46-399 ==> 0-353 on |142|IGHV3-48|L-REGION+V-REGION| [len=353] (mis=12)
428-476 ==> 0-48 on |55|IGHJ4|J-REGION| [len=48] (mis=0)
junction support: 2 umis using 26 reads
cdr3 = CARPARTSGGATFDYFDYW at 388, score = 8 + 7
umis assigned: [244, 298, 504]
of which 3 are surviving nonsolos
reads assigned: 414
start codons at 46, 202, 323, 349, 600
confident = true

TIG 2[bases=569]
0-30 ==> 145-175 on |295|IGKV4-1|5'UTR| [len=175] (mis=0)
30-393 ==> 0-363 on |296|IGKV4-1|L-REGION+V-REGION| [len=363] (mis=0)
396-433 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=0)
433-569 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 9 umis using 204 reads
cdr3 = CQQYYSTPSLTF at 369, score = 9 + 9
umis assigned: [127, 290, 411, 436, 452, 479, 480, 523, 540, 679] and 1 others
of which 11 are surviving nonsolos
reads assigned: 1723
start codons at 30, 99, 352, 475
confident = true

REJECT CONTIGS

TIG 1[bases=394]
4-187 ==> 169-352 on rc of segment before IGHJ4 exon 1 [len=352] (mis=0)
185-236 ==> 0-51 on |57|IGHJ5|J-REGION| [len=51] (mis=0)
236-394 ==> 0-158 on rc of segment before IGHJ5 exon 1 [len=348] (mis=0)
umis assigned: [577]
of which 1 are surviving nonsolos
reads assigned: 55
start codons at 6, 78, 180, 360
confident = false
did not find CDR3
now this is a cell
paired!

GCTGTGTATTACTGTGCGAGGCCAGCGCGAACTTCCGGGGGAGCTACTTTCGACTACTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG <==> AGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATAGTACTCCTTCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAAC

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 81.215 = GTCATTTCACACCGAC-1

using 253 reads

====================================================================================

graph has 114 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 1[249]
surviving nonsolo ucounts = 1[249]
ids = [1]

====================================================================================

UMI info for barcode GTCATTTCACACCGAC-1 contig 1 = TGATCAGGAC...
umi ACAATGCGTT = 229 reads: +397 validated

GOOD CONTIGS

TIG 1[bases=511]
0-31 ==> 5-36 on |272|IGKV2D-28|5'UTR| [len=36] (mis=0)
31-391 ==> 0-360 on |273|IGKV2D-28|L-REGION+V-REGION| [len=360] (mis=2)
390-428 ==> 0-38 on |215|IGKJ3|J-REGION| [len=38] (mis=0)
428-511 ==> 0-83 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 48 reads
cdr3 = CMQALQTPFTF at 367, score = 9 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 225
start codons at 31, 64, 100, 188, 350, 370, 470
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 81.226 = GTCATTTCAGGCTGAA-1

using 231 reads

====================================================================================

graph has 76 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[6, 223]
surviving nonsolo ucounts = 1[223]
ids = [3]

====================================================================================

UMI info for barcode GTCATTTCAGGCTGAA-1 contig 1 = AGGAGTCAGA...
umi GTTCAACTAC = 207 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=460]
0-27 ==> 154-181 on |232|IGKV1-5|5'UTR| [len=181] (mis=2)
27-378 ==> 0-351 on |233|IGKV1-5|L-REGION+V-REGION| [len=351] (mis=33)
377-415 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=5)
415-460 ==> 0-45 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 45 reads
cdr3 = CQQYHSYSPTF at 354, score = 8 + 8
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 205
start codons at 27, 33, 89, 102, 238, 241, 334
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 81.228 = GTCATTTCAGTTTACG-1

using 119 reads

====================================================================================

graph has 48 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[118]
surviving nonsolo ucounts = 1[118]
ids = [0]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 81.232 = GTCATTTCATCGTCGG-1

using 252 reads

====================================================================================

graph has 53 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 2[2, 246]
surviving nonsolo ucounts = 1[246]
ids = [3]

====================================================================================

UMI info for barcode GTCATTTCATCGTCGG-1 contig 1 = GGAGTCAGTC...
umi ATCCCGTTCT = 248 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=556]
0-26 ==> 32-58 on |238|IGKV1-9|5'UTR| [len=58] (mis=0)
26-377 ==> 0-351 on |239|IGKV1-9|L-REGION+V-REGION| [len=351] (mis=1)
382-420 ==> 0-38 on |215|IGKJ3|J-REGION| [len=38] (mis=1)
420-556 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 37 reads
cdr3 = CQQLNSYPRRITF at 353, score = 8 + 8
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 245
start codons at 26, 32, 88, 237, 462
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 81.239 = GTCATTTGTAAACACA-1

using 225 reads

====================================================================================

graph has 72 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 1[222]
surviving nonsolo ucounts = 1[222]
ids = [0]

====================================================================================

UMI info for barcode GTCATTTGTAAACACA-1 contig 1 = AGCTGTGGGC...
umi CATGGCCCCC = 213 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=537]
0-40 ==> 0-40 on |356|IGLV3-19|5'UTR| [len=40] (mis=0)
40-377 ==> 0-337 on |357|IGLV3-19|L-REGION+V-REGION| [len=337] (mis=19)
384-422 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=1)
422-537 ==> 0-115 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 40 reads
cdr3 = CNSRDSSGNEVVF at 355, score = 7 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 209
start codons at 40, 159, 188, 239, 338
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 81.248 = GTCATTTGTCACCCAG-1

using 233 reads

====================================================================================

graph has 94 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[233]
surviving nonsolo ucounts = 1[233]
ids = [0]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 81.252 = GTCATTTGTCCGAAGA-1

using 8408 reads

====================================================================================

graph has 3890 edges initially, 30 edges after simplification

total ucounts = 675
nonsolo ucounts = 325[2^109, 3^78, 4^33, 5^33, 6^16, 7^10, 8^6, 9^6, 10^4, 11, 13, 15, 17, 21, 65, 150, 207, 224, 228, 235, 242, 249, 250, 267, 271, 274, 279, 281, 289, 290, 306, 308, 311, 331, 339, 341, 342, 377, 468]
surviving nonsolo ucounts = 25[65, 150, 207, 224, 228, 235, 242, 249, 250, 267, 271, 274, 279, 281, 289, 290, 306, 308, 311, 331, 339, 341, 342, 377, 468]
ids = [106, 611, 340, 293, 621, 6, 488, 471, 614, 425, ...]

====================================================================================

UMI info for barcode GTCATTTGTCCGAAGA-1 contig 1 = AGGAGTCAGT...
umi ACACATACAC = 237 reads: +388 validated
umi ACTCAACGGG = 469 reads: +388 validated
umi ACTTATCGCA = 336 reads: +388 validated
umi ATACTAATGT = 327 reads: +388 validated
umi ATATCCGGTA = 311 reads: +388 validated
umi ATGATTAGGG = 338 reads: +388 validated
umi CAAGACCCTA = 304 reads: +388 validated
umi CACAAATTGG = 68 reads: +388 validated
umi CCACCCCGAT = 276 reads: +388 validated
umi CTGTCTGCAC = 234 reads: +56 -1XX +1 -1XX +4 -5XX +4 -20XX +1 -3XX +3 -11XX +278 invalidated
umi GAACCATAGC = 306 reads: +388 validated
umi GCACTTCCTC = 210 reads: +388 validated
umi GCGATGATAC = 294 reads: +388 validated
umi GTATTGTTAA = 273 reads: +388 validated
umi TAAAATTACG = 340 reads: +388 validated
umi TAGACCAGGA = 248 reads: +388 validated
umi TGAGAACCGC = 379 reads: +388 validated
umi TGGCTAATAT = 281 reads: +388 validated
umi TTAGTGCCAT = 153 reads: +388 validated
umi TTATAGACGG = 245 reads: +388 validated
umi TTCAATGAGT = 227 reads: +388 validated

UMI info for barcode GTCATTTGTCCGAAGA-1 contig 2 = GGGAGGGTCC...
umi CTGACTGGAC = 242 reads: +427 validated
umi GTCACGCTTT = 266 reads: +427 validated
umi GTCTCTAGAG = 287 reads: +427 validated
umi TATGATCGGG = 245 reads: +427 validated

GOOD CONTIGS

TIG 1[bases=551]
0-27 ==> 4-31 on |226|IGKV1-33|5'UTR| [len=31] (mis=0)
27-378 ==> 0-351 on |227|IGKV1-33|L-REGION+V-REGION| [len=351] (mis=2)
377-415 ==> 0-38 on |217|IGKJ5|J-REGION| [len=38] (mis=0)
415-551 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 21 umis using 958 reads
cdr3 = CQQYDNLLITF at 354, score = 9 + 8
umis assigned: [6, 34, 40, 59, 62, 75, 98, 106, 159, 293] and 11 others
of which 21 are surviving nonsolos
reads assigned: 5767
start codons at 27, 33, 89, 102, 241, 364, 457
confident = true

TIG 2[bases=559]
61-409 ==> 0-348 on |740|IGHV4-30-4|L-REGION+V-REGION| [len=348] (mis=5)
442-488 ==> 0-46 on |54|IGHJ4|J-REGION| [len=46] (mis=4)
488-559 ==> 0-71 on |60|IGHM|C-REGION| [len=1358] (mis=0)
junction support: 4 umis using 102 reads
cdr3 = CARGPRRGRGITGCDYW at 406, score = 9 + 7
umis assigned: [285, 425, 431, 488]
of which 4 are surviving nonsolos
reads assigned: 1027
start codons at 17, 61, 105
confident = true
now this is a cell
paired!

GACACGGCCGTGTATTACTGTGCGAGGGGGCCCCGGCGGGGTCGGGGAATTACTGGGTGTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG <==> ATCAGCAGCCTGCAGCCTGAAGATATTGCAACATATTACTGTCAACAGTATGATAATCTCCTGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAAC

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 81.256 = GTCATTTGTCGACTAT-1

using 218 reads

====================================================================================

graph has 62 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[2^2, 213]
surviving nonsolo ucounts = 1[213]
ids = [0]

====================================================================================

UMI info for barcode GTCATTTGTCGACTAT-1 contig 1 = GCTGGGGTCT...
umi ATGGCCCACA = 203 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=582]
0-41 ==> 122-163 on |346|IGLV2-8|5'UTR| [len=163] (mis=0)
41-384 ==> 0-343 on |347|IGLV2-8|L-REGION+V-REGION| [len=354] (mis=7)
391-429 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=1)
429-582 ==> 0-153 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 37 reads
cdr3 = CNSYAGNKIEVF at 365, score = 8 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 201
start codons at 41, 242, 249, 348, 375
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 81.257 = GTCATTTGTCTAGTCA-1

using 267 reads

====================================================================================

graph has 94 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[2^2, 3, 258]
surviving nonsolo ucounts = 1[258]
ids = [0]

====================================================================================

UMI info for barcode GTCATTTGTCTAGTCA-1 contig 1 = GGTGCTTTCT...
umi ATCAAACCTG = 251 reads: +451 validated

GOOD CONTIGS

TIG 1[bases=540]
17-388 ==> 0-371 on |186|IGHV4-34|L-REGION+V-REGION| [len=371] (mis=11)
420-468 ==> 0-48 on |55|IGHJ4|J-REGION| [len=48] (mis=1)
468-540 ==> 0-72 on |1|IGHA1|C-REGION| [len=1058] (mis=0)
junction support: 1 umis using 27 reads
cdr3 = CARGSGILVIAIEDYYFDHW at 377, score = 9 + 7
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 249
start codons at 17, 38, 82, 168, 255, 522
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 81.265 = GTCATTTGTGAGGCTA-1

using 249 reads

====================================================================================

graph has 102 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 3[3, 6, 240]
surviving nonsolo ucounts = 1[240]
ids = [1]

====================================================================================

UMI info for barcode GTCATTTGTGAGGCTA-1 contig 1 = AGAGCTGCTC...
umi CAATGTCACG = 226 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=476]
0-31 ==> 21-52 on |282|IGKV3-20|5'UTR| [len=52] (mis=0)
31-379 ==> 0-348 on |283|IGKV3-20|L-REGION+V-REGION| [len=348] (mis=0)
380-419 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=4)
419-476 ==> 0-57 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 29 reads
cdr3 = CQQYGSSPRVTF at 355, score = 9 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 221
start codons at 31, 239, 365, 461
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 81.268 = GTCATTTGTGGCTCCA-1

using 6013 reads

====================================================================================

graph has 3445 edges initially, 32 edges after simplification

total ucounts = 758
nonsolo ucounts = 371[2^145, 3^84, 4^46, 5^27, 6^18, 7^7, 8^9, 9^3, 10^2, 11^2, 12^2, 13^2, 16^2, 17^2, 18, 20, 55, 62, 75, 126, 162, 174, 180, 203, 276, 290, 295, 311, 317, 334^2, 345, 368, 406]
surviving nonsolo ucounts = 18[18, 55, 62, 75, 126, 162, 174, 180, 203, 276, 290, 295, 311, 317, 334, 345, 368, 406]
ids = [269, 338, 95, 294, 82, 12, 440, 94, 243, 85, ...]

====================================================================================

UMI info for barcode GTCATTTGTGGCTCCA-1 contig 1 = AGAGCTCTGG...
umi AAATGGAGCC = 321 reads: +385 validated
umi ACGTTCATAG = 128 reads: +385 validated
umi ACTCGGCCTA = 283 reads: +385 validated
umi AGAGTCGCGT = 181 reads: +385 validated
umi AGAGTCGCTT = 63 reads: +40 -3 +342 non-validated
umi AGCTTGGGTT = 315 reads: +385 validated
umi ATGGTTCACT = 348 reads: +353 -1XX +31 invalidated
umi CCAATCTTTT = 206 reads: +385 validated
umi CCCCCTGTGC = 358 reads: +53 -1XX +29 -1XX +31 -1XX +26 -1XX +3 -1XX +1 -1XX +2 -1XX +24 -1XX +44 -1XX +8 -1XX +6 -1XX +1 -4XX +5 -1XX +23 -1XX +4 -1XX +36 -2XX +7 -1XX +5 -1XX +2 -1XX +2 -1XX +1 -2XX +3 -1XX +3 -2 +1 -2XX +2 -1XX +2 -1XX +5 -1XX +7 -1XX +6 -1XX +4 -2XX +1 invalidated
umi CGAAAGCGGT = 75 reads: +385 validated
umi CGCTCCTTTT = 1 reads: -385 non-validated
umi GACTCTGCTC = 411 reads: +385 validated
umi GCTTGATATG = 364 reads: +385 validated
umi GTCTATGCCT = 335 reads: +385 validated
umi TCAAGCCTTC = 290 reads: +385 validated
umi TTAGTTCGCT = 294 reads: +385 validated

UMI info for barcode GTCATTTGTGGCTCCA-1 contig 2 = AGCATCATCC...
umi CCCTCTTTTA = 18 reads: +11 -66 +175 -1 +139 -47 non-validated
umi CTAACCGCCC = 56 reads: +384 -1 +34 -1 +8 -1 +5 -1 +1 -1 +2 non-validated
umi GCACCTTCTT = 172 reads: +439 validated

GOOD CONTIGS

TIG 1[bases=565]
0-44 ==> 8-52 on |282|IGKV3-20|5'UTR| [len=52] (mis=0)
44-358 ==> 0-314 on |283|IGKV3-20|L-REGION+V-REGION| [len=348] (mis=18)
390-429 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=8)
429-565 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 13 umis using 521 reads
cdr3 = CHQYASTPFTF at 368, score = 8 + 8
umis assigned: [18, 82, 85, 94, 95, 106, 168, 243, 259, 294] and 6 others
of which 14 are surviving nonsolos
reads assigned: 3838
start codons at 44, 252, 359, 471
confident = true

TIG 2[bases=593]
61-414 ==> 0-353 on |73|IGHV1-3|L-REGION+V-REGION| [len=353] (mis=59)
463-500 ==> 15-52 on |49|IGHJ1|J-REGION| [len=52] (mis=2)
500-593 ==> 0-93 on |45|IGHG3|C-REGION| [len=1130] (mis=0)
junction support: 2 umis using 26 reads
cdr3 = CARGGAPNISGHGSFYSVGDHW at 403, score = 8 + 7
umis assigned: [269, 338, 440]
of which 3 are surviving nonsolos
reads assigned: 240
start codons at 61, 265, 358
confident = true
now this is a cell
paired!

TACTGTGCGAGAGGCGGAGCTCCGAATATCTCCGGTCACGGCAGCTTTTATTCCGTTGGCGACCACTGGGGCCAGGGGACCCTCGTCACCGTCTCCTCAG <==> ATCAGCAGACTGGAGCCTGAAGATTTTGCCATGTATTATTGTCACCAATACGCGAGCACACCGTTTACTTTTGGCCAGGGGACCAGGGTGGAGATCACTC

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BARCODE 81.275 = GTCATTTGTTCGAATC-1

using 11 reads

====================================================================================

graph has 4 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[11]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!
sorting bam, mem = 0.10
running samtools sort -l 8G -o /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk081-ucc6a7a72ce/files/contig_bam_sorted.bam /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk081-ucc6a7a72ce/files/contig_bam.bam

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19.310 seconds used processing barcodes, peak mem = 0.24
