[stdout]

starting vdj_asm_asm, mem = 0.01
available mem = 833.92 GB
/scratch/etanis/software/cellranger-6.1.2/lib/bin/cr_vdj martian assembly main /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk074-ucc6a7a72ce /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk074-ucc6a7a72ce/files /scratch/etanis/ST-118/multi/dimitri-multi/journal/SC_MULTI_CS.SC_MULTI_CORE.MULTI_GEM_WELL_PROCESSOR.VDJ_B_GEM_WELL_PROCESSOR.SC_VDJ_CONTIG_ASSEMBLER.ASSEMBLE_VDJ.fork0.chnk074.ucc6a7a72ce
n50_n50_rpu = 288

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 74.0 = GGACGTCAGTACTTGC-1

using 824 reads

====================================================================================

graph has 326 edges initially, 8 edges after simplification

total ucounts = 7
nonsolo ucounts = 5[2, 6, 268, 271, 275]
surviving nonsolo ucounts = 3[268, 271, 275]
ids = [2, 6, 0]

====================================================================================

UMI info for barcode GGACGTCAGTACTTGC-1 contig 1 = GGAGGAACTG...
umi GTTGGGAACA = 270 reads: +385 validated

UMI info for barcode GGACGTCAGTACTTGC-1 contig 2 = GATCAGGACT...
umi CCCAAGGTTG = 271 reads: +397 validated

UMI info for barcode GGACGTCAGTACTTGC-1 contig 3 = ATCACATAAC...
umi ACGGATCCGT = 265 reads: +436 validated

GOOD CONTIGS

TIG 1[bases=555]
0-34 ==> 63-97 on |280|IGKV3-15|5'UTR| [len=97] (mis=0)
34-379 ==> 0-345 on |281|IGKV3-15|L-REGION+V-REGION| [len=345] (mis=18)
380-419 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=3)
419-555 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 45 reads
cdr3 = CQQYYNWPPYTF at 355, score = 9 + 8
umis assigned: [6]
of which 1 are surviving nonsolos
reads assigned: 265
start codons at 34, 103, 239, 461
confident = false

TIG 2[bases=563]
0-30 ==> 0-30 on |264|IGKV2-28|5'UTR| [len=30] (mis=0)
30-390 ==> 0-360 on |265|IGKV2-28|L-REGION+V-REGION| [len=360] (mis=0)
389-427 ==> 0-38 on |217|IGKJ5|J-REGION| [len=38] (mis=3)
427-563 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 37 reads
cdr3 = CMQALQTPDTF at 366, score = 9 + 8
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 264
start codons at 30, 63, 99, 187, 349, 369, 469
confident = false

TIG 3[bases=535]
0-58 ==> 0-58 on |87|IGHV1-69D|5'UTR| [len=58] (mis=0)
58-411 ==> 0-353 on |88|IGHV1-69D|L-REGION+V-REGION| [len=353] (mis=12)
412-430 ==> 0-18 on |35|IGHD6-6|D-REGION| [len=18] (mis=1)
431-494 ==> 0-63 on |737|IGHJ6|J-REGION| [len=63] (mis=3)
494-535 ==> 0-41 on |40|IGHG1|C-REGION| [len=884] (mis=0)
junction support: 1 umis using 20 reads
cdr3 = CARVGYSSSSSYHYYYAMDVW at 400, score = 9 + 7
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 262
start codons at 58, 209, 256, 355, 451
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 74.3 = GGACGTCAGTGAATTG-1

using 205 reads

====================================================================================

graph has 104 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 2[3, 199]
surviving nonsolo ucounts = 1[199]
ids = [2]

====================================================================================

UMI info for barcode GGACGTCAGTGAATTG-1 contig 1 = TCTGGCGCCA...
umi CTAACTACTA = 201 reads: +385 validated

GOOD CONTIGS

TIG 1[bases=630]
0-34 ==> 0-34 on |385|IGLV7-43|5'UTR| [len=34] (mis=1)
34-364 ==> 0-330 on |386|IGLV7-43|L-REGION+V-REGION| [len=351] (mis=36)
381-419 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=4)
419-630 ==> 0-211 on |307|IGLC3|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 34 reads
cdr3 = CLIYFGDSWVF at 358, score = 8 + 8
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 198
start codons at 34
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 74.11 = GGACGTCAGTTTCCTT-1

using 256 reads

====================================================================================

graph has 62 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 1[247]
surviving nonsolo ucounts = 1[247]
ids = [6]

====================================================================================

UMI info for barcode GGACGTCAGTTTCCTT-1 contig 1 = GAATCAGTCC...
umi CTTTCCCTTA = 250 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=549]
0-25 ==> 2-27 on |222|IGKV1-17|5'UTR| [len=27] (mis=0)
25-376 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
375-413 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
413-549 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 38 reads
cdr3 = CLQYSSSPWTF at 352, score = 9 + 8
umis assigned: [6]
of which 1 are surviving nonsolos
reads assigned: 247
start codons at 25, 31, 100, 236, 455
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 74.13 = GGACGTCCAAACGCGA-1

using 341 reads

====================================================================================

graph has 164 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[340]
surviving nonsolo ucounts = 1[340]
ids = [0]

====================================================================================

UMI info for barcode GGACGTCCAAACGCGA-1 contig 1 = GCTACAACAG...
umi ACCATAAGGC = 295 reads: +400 validated

GOOD CONTIGS

TIG 1[bases=507]
0-28 ==> 147-175 on |295|IGKV4-1|5'UTR| [len=175] (mis=0)
28-391 ==> 0-363 on |296|IGKV4-1|L-REGION+V-REGION| [len=363] (mis=15)
390-428 ==> 0-38 on |217|IGKJ5|J-REGION| [len=38] (mis=3)
428-507 ==> 0-79 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 49 reads
cdr3 = CQQYYTTPVTF at 367, score = 9 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 291
start codons at 28, 170, 470
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 74.20 = GGACGTCCACAAGACG-1

using 369 reads

====================================================================================

graph has 134 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 1[365]
surviving nonsolo ucounts = 1[365]
ids = [3]

====================================================================================

UMI info for barcode GGACGTCCACAAGACG-1 contig 1 = GGGGAGGAAC...
umi TCCTTGATTT = 362 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=554]
0-36 ==> 11-47 on |278|IGKV3-11|5'UTR| [len=47] (mis=0)
36-381 ==> 0-345 on |279|IGKV3-11|L-REGION+V-REGION| [len=345] (mis=3)
381-418 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=0)
418-554 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 71 reads
cdr3 = CQQRSNWPLTF at 357, score = 9 + 9
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 356
start codons at 36, 244, 460
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 74.23 = GGACGTCCACGAGAGT-1

using 45 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[3, 42]
surviving nonsolo ucounts = 1[42]
ids = [0]

====================================================================================

REJECT CONTIGS

TIG 1[bases=429]
0-339 ==> 1-340 on |343|IGLV2-23|L-REGION+V-REGION| [len=340] (mis=0)
352-390 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=0)
390-429 ==> 0-39 on |306|IGLC2|C-REGION| [len=317] (mis=0)
cdr3 = CCSYAGSSTHVVF at 323, score = 8 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 36
start codons at 138, 200, 207, 333, 351
confident = false
not full
VJ delta = 6
not full
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 74.25 = GGACGTCCACTACAGT-1

using 268 reads

====================================================================================

graph has 90 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 1[265]
surviving nonsolo ucounts = 1[265]
ids = [2]

====================================================================================

UMI info for barcode GGACGTCCACTACAGT-1 contig 1 = AGAGCTCTGG...
umi CGCTTCCCAC = 2 reads: -111 +4 -1 +2 -1 +6 -1 +1 -1 +6 -2 +2 -1X +22 -1 +1 -1X +3 -221 invalidated
umi CGCTTCCGAG = 241 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=511]
0-44 ==> 8-52 on |282|IGKV3-20|5'UTR| [len=52] (mis=0)
44-392 ==> 0-348 on |283|IGKV3-20|L-REGION+V-REGION| [len=348] (mis=1)
393-432 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=2)
432-511 ==> 0-79 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 34 reads
cdr3 = CQQYGSSPLYTF at 368, score = 9 + 8
umis assigned: [1, 2]
of which 1 are surviving nonsolos
reads assigned: 238
start codons at 44, 252, 378, 474
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 74.27 = GGACGTCCAGATCCAT-1

using 628 reads

====================================================================================

graph has 208 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[2, 198, 426]
surviving nonsolo ucounts = 2[198, 426]
ids = [4, 1]

====================================================================================

UMI info for barcode GGACGTCCAGATCCAT-1 contig 1 = GGGGTCTCAG...
umi GCATTGTCAG = 427 reads: +388 validated
umi TGGCTATCAG = 197 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=637]
0-38 ==> 4-42 on |336|IGLV2-11|5'UTR| [len=42] (mis=1)
38-382 ==> 0-344 on |337|IGLV2-11|L-REGION+V-REGION| [len=358] (mis=9)
388-426 ==> 0-38 on |309|IGLJ1|J-REGION| [len=38] (mis=2)
426-637 ==> 0-211 on |305|IGLC1|C-REGION| [len=317] (mis=0)
junction support: 2 umis using 83 reads
cdr3 = CCSFAGRYESVF at 362, score = 7 + 8
umis assigned: [1, 4]
of which 2 are surviving nonsolos
reads assigned: 615
start codons at 38, 177, 239, 246, 249, 345, 558
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 74.30 = GGACGTCCAGGGAGAG-1

using 254 reads

====================================================================================

graph has 108 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[2, 251]
surviving nonsolo ucounts = 1[251]
ids = [2]

====================================================================================

UMI info for barcode GGACGTCCAGGGAGAG-1 contig 1 = GAATCAGTCC...
umi GCTGGTAATC = 253 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=549]
0-25 ==> 2-27 on |222|IGKV1-17|5'UTR| [len=27] (mis=0)
25-376 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
375-413 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
413-549 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 33 reads
cdr3 = CLQYSSSPWTF at 352, score = 9 + 8
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 248
start codons at 25, 31, 100, 236, 455
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 74.34 = GGACGTCCATAGTAAG-1

using 287 reads

====================================================================================

graph has 118 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 1[285]
surviving nonsolo ucounts = 1[285]
ids = [0]

====================================================================================

UMI info for barcode GGACGTCCATAGTAAG-1 contig 1 = GAGGAATCAG...
umi AACGATATTA = 249 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=489]
28-379 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
378-416 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
416-489 ==> 0-73 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 40 reads
cdr3 = CLQYSSSPWTF at 355, score = 9 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 247
start codons at 28, 34, 103, 239, 458
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 74.48 = GGACGTCGTCATACTG-1

using 118 reads

====================================================================================

graph has 94 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 1[116]
surviving nonsolo ucounts = 1[116]
ids = [1]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 74.49 = GGACGTCGTCATCGGC-1

using 59 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[58]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================

UMI info for barcode GGACGTCGTCATCGGC-1 contig 1 = AGGGTCCTGC...
umi CTCGTACGGT = 56 reads: +436 validated

GOOD CONTIGS

TIG 1[bases=502]
58-414 ==> 0-356 on |196|IGHV4-61|L-REGION+V-REGION| [len=356] (mis=42)
431-494 ==> 0-63 on |737|IGHJ6|J-REGION| [len=63] (mis=8)
junction support: 1 umis using 8 reads
cdr3 = CARGREYASGQDYYYGMDVW at 403, score = 9 + 7
umis assigned: [1]
of which 0 are surviving nonsolos
reads assigned: 55
start codons at 14, 58, 102, 281, 422, 451
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 74.50 = GGACGTCGTCCAAGTT-1

using 195 reads

====================================================================================

graph has 70 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[2, 5, 187]
surviving nonsolo ucounts = 1[187]
ids = [0]

====================================================================================

UMI info for barcode GGACGTCGTCCAAGTT-1 contig 1 = GGAGTCAGAC...
umi AACAACACAA = 162 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=434]
0-26 ==> 21-47 on |218|IGKV1-12|5'UTR| [len=47] (mis=0)
26-377 ==> 0-351 on |219|IGKV1-12|L-REGION+V-REGION| [len=351] (mis=12)
377-414 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=0)
414-434 ==> 0-20 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 33 reads
cdr3 = CQQANSFPLTF at 353, score = 9 + 9
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 160
start codons at 26, 32, 88, 101, 240, 258
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 74.54 = GGACGTCGTCCCTTGT-1

using 134 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[3, 129]
surviving nonsolo ucounts = 1[129]
ids = [0]

====================================================================================

UMI info for barcode GGACGTCGTCCCTTGT-1 contig 1 = TCTGAGGATA...
umi ACCGCTCCAT = 119 reads: +391 validated

GOOD CONTIGS

TIG 1[bases=543]
0-86 ==> 0-86 on |732|IGLV6-57|5'UTR| [len=86] (mis=0)
86-439 ==> 0-353 on |384|IGLV6-57|L-REGION+V-REGION| [len=353] (mis=1)
439-477 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=0)
477-543 ==> 0-66 on |307|IGLC3|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 19 reads
cdr3 = CQSYDSSNRWVF at 413, score = 6 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 118
start codons at 86, 149, 240, 291, 423
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 74.68 = GGACGTCGTTGTTTGG-1

using 83 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[2, 81]
surviving nonsolo ucounts = 1[81]
ids = [1]

====================================================================================

UMI info for barcode GGACGTCGTTGTTTGG-1 contig 1 = GCTCTGCTTC...
umi ATACCGCGCG = 77 reads: +391 validated

GOOD CONTIGS

TIG 1[bases=533]
0-51 ==> 0-51 on |320|IGLV1-40|5'UTR| [len=51] (mis=0)
51-407 ==> 0-356 on |321|IGLV1-40|L-REGION+V-REGION| [len=356] (mis=12)
404-442 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=3)
442-533 ==> 0-91 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 12 reads
cdr3 = CQSYDTSLSGSVF at 375, score = 8 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 76
start codons at 51, 205, 208, 259, 358, 385
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 74.71 = GGACGTCTCAACCAAC-1

using 219 reads

====================================================================================

graph has 112 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 4[2, 4^2, 208]
surviving nonsolo ucounts = 1[208]
ids = [3]

====================================================================================

UMI info for barcode GGACGTCTCAACCAAC-1 contig 1 = GAGAGAGGAG...
umi TCGTCCCCAG = 204 reads: +424 validated

GOOD CONTIGS

TIG 1[bases=544]
0-73 ==> 6-79 on |119|IGHV3-21|5'UTR| [len=79] (mis=0)
73-426 ==> 0-353 on |120|IGHV3-21|L-REGION+V-REGION| [len=353] (mis=18)
450-497 ==> 16-63 on |737|IGHJ6|J-REGION| [len=63] (mis=1)
497-544 ==> 0-47 on |43|IGHG2|C-REGION| [len=977] (mis=0)
junction support: 1 umis using 18 reads
cdr3 = CARDRAATARLGGMDVW at 415, score = 9 + 7
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 199
start codons at 73, 224, 229, 376, 454
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 74.86 = GGACGTCTCATCGCTC-1

using 204 reads

====================================================================================

graph has 62 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[2^2, 199]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 74.88 = GGACGTCTCATGCATG-1

using 13 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[13]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 74.98 = GGACGTCTCGTACCGG-1

using 431 reads

====================================================================================

graph has 162 edges initially, 6 edges after simplification

total ucounts = 10
nonsolo ucounts = 3[4, 133, 287]
surviving nonsolo ucounts = 2[133, 287]
ids = [6, 4]

====================================================================================

UMI info for barcode GGACGTCTCGTACCGG-1 contig 1 = CACCTTCTCA...
umi GGCTCTAATC = 134 reads: +397 validated

UMI info for barcode GGACGTCTCGTACCGG-1 contig 2 = GAAGAGCTGC...
umi ATGGGAGCTT = 289 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=545]
12-372 ==> 0-360 on |265|IGKV2-28|L-REGION+V-REGION| [len=360] (mis=0)
371-409 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=2)
409-545 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 21 reads
cdr3 = CMQALQTPRTF at 348, score = 9 + 8
umis assigned: [6]
of which 1 are surviving nonsolos
reads assigned: 133
start codons at 12, 45, 81, 169, 331, 351, 451
confident = false

TIG 2[bases=557]
0-33 ==> 19-52 on |282|IGKV3-20|5'UTR| [len=52] (mis=1)
33-381 ==> 0-348 on |283|IGKV3-20|L-REGION+V-REGION| [len=348] (mis=26)
382-421 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=4)
421-557 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 35 reads
cdr3 = CQQYGSSPMYTF at 357, score = 9 + 8
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 286
start codons at 33, 82, 241, 340, 381, 463
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 74.106 = GGACGTCTCTCAAACG-1

using 666 reads

====================================================================================

graph has 214 edges initially, 2 edges after simplification

total ucounts = 14
nonsolo ucounts = 5[2^3, 316, 335]
surviving nonsolo ucounts = 2[316, 335]
ids = [5, 13]

====================================================================================

UMI info for barcode GGACGTCTCTCAAACG-1 contig 1 = GGGAATCAGT...
umi GAAAACCCAG = 318 reads: +388 validated
umi TTGTTGCGCT = 333 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=551]
0-27 ==> 0-27 on |222|IGKV1-17|5'UTR| [len=27] (mis=1)
27-378 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
377-415 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
415-551 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 2 umis using 98 reads
cdr3 = CLQYSSSPWTF at 354, score = 9 + 8
umis assigned: [5, 13]
of which 2 are surviving nonsolos
reads assigned: 642
start codons at 27, 33, 102, 238, 457
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 74.123 = GGAGCAAAGATGTTAG-1

using 1437 reads

====================================================================================

graph has 2092 edges initially, 10 edges after simplification

total ucounts = 638
nonsolo ucounts = 264[2^98, 3^59, 4^38, 5^18, 6^16, 7^12, 8^6, 9^6, 10^3, 11^3, 12, 14, 17, 19, 41]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 74.124 = GGAGCAAAGCAGGTCA-1

using 269 reads

====================================================================================

graph has 122 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[269]
surviving nonsolo ucounts = 1[269]
ids = [0]

====================================================================================

UMI info for barcode GGAGCAAAGCAGGTCA-1 contig 1 = AGCTTCAGCT...
umi CCACGCTCTA = 253 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=578]
0-47 ==> 67-114 on |322|IGLV1-44|5'UTR| [len=114] (mis=0)
47-400 ==> 0-353 on |323|IGLV1-44|L-REGION+V-REGION| [len=353] (mis=15)
397-435 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=3)
435-578 ==> 0-143 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 40 reads
cdr3 = CASWDDSLRGRVF at 368, score = 8 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 246
start codons at 47, 201, 351, 376, 381
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 74.126 = GGAGCAAAGCGTAGTG-1

using 8191 reads

====================================================================================

graph has 3025 edges initially, 68 edges after simplification

total ucounts = 204
nonsolo ucounts = 97[2^24, 3^14, 4^4, 5^12, 6^5, 8, 9, 10, 11, 37, 45, 63, 75, 105, 116, 118, 131, 137, 148, 169, 184, 192, 210, 222, 223, 230, 250, 253, 259, 262, 266, 270^2, 274, 275, 289, 298, 317, 318, 380, 404, 439, 620]
surviving nonsolo ucounts = 32[37, 63, 75, 105, 116, 118, 131, 137, 148, 169, 184, 192, 210, 222, 223, 230, 250, 253, 259, 262, 266, 270^2, 274, 275, 289, 298, 317, 318, 380, 404, 439]
ids = [44, 170, 9, 41, 15, 49, 153, 132, 36, 124, ...]

====================================================================================

UMI info for barcode GGAGCAAAGCGTAGTG-1 contig 1 = AGCTTCAGCT...
umi AAATGCCCGT = 640 reads: -344X +1 -1X +1 -2X +2 -2XX +3 -1XX +5 -4XX +9 -1XX +1 -1XX +10 invalidated
umi AAGCATCGGA = 251 reads: +388 validated
umi ATCTTTCGTT = 118 reads: +388 validated
umi CCTGCCGCCG = 263 reads: +388 validated
umi GAGCTTGCGA = 191 reads: +388 validated
umi GCTCCTATAG = 46 reads: -352X +1 -1X +1 -4XX +2 -3XX +1 -1XX +1 -4XX +1 -3XX +1 -1XX +1 -3XX +1 -4XX +2 invalidated
umi GCTCGTCTCA = 274 reads: +388 validated
umi GTCATTCCTT = 121 reads: +388 validated
umi GTGTGCGCCT = 444 reads: +388 validated

UMI info for barcode GGAGCAAAGCGTAGTG-1 contig 2 = GGGAGAGGAG...
umi AAACTTATCA = 225 reads: +421 validated
umi AAGAATCACA = 73 reads: +350 -1 +5 -65 non-validated
umi AATTCATCTC = 94 reads: +421 validated
umi ACAAGACTTG = 183 reads: +416 -1 +4 non-validated
umi ACAGATCTTG = 262 reads: +421 validated
umi AGGAATTTCC = 132 reads: -366X +1 -3X +1 -4X +1 -2X +2 -1X +3 -2XX +35 invalidated
umi AGTCATTGGG = 276 reads: -144X +2 -1 +274 invalidated
umi ATACGGCCCG = 107 reads: +393 -1 +7 -1 +6 -1 +3 -1 +5 -2 +1 non-validated
umi ATCCGATCCA = 37 reads: +352 -19 +50 non-validated
umi CAAGCGTGGG = 272 reads: +421 validated
umi CACATATACC = 272 reads: +421 validated
umi CCAAGCCGGG = 289 reads: +421 validated
umi CTAATTGCTC = 299 reads: +421 validated
umi GCAAACCGCC = 169 reads: +421 validated
umi GCACCCCAGG = 322 reads: +421 validated
umi GCGTAAATCT = 230 reads: +421 validated
umi GCGTCATCTC = 143 reads: +123 -1XX +282 -12 +3 invalidated
umi GGGCTCAGCA = 214 reads: +421 validated
umi TATCGATGGC = 63 reads: +353 -1 +27 -7 +33 non-validated

UMI info for barcode GGAGCAAAGCGTAGTG-1 contig 3 = AGAGCTCTGG...
umi CCGCACCTCT = 405 reads: +388 validated
umi CCTTTATCTT = 267 reads: +388 validated
umi GAGTCACATA = 251 reads: +388 validated
umi GTGAGTCTCA = 271 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=646]
0-47 ==> 67-114 on |322|IGLV1-44|5'UTR| [len=114] (mis=0)
47-400 ==> 0-353 on |323|IGLV1-44|L-REGION+V-REGION| [len=353] (mis=1)
397-435 ==> 0-38 on |309|IGLJ1|J-REGION| [len=38] (mis=2)
435-646 ==> 0-211 on |305|IGLC1|C-REGION| [len=317] (mis=0)
junction support: 7 umis using 251 reads
cdr3 = CAAWDDSLNGQVF at 368, score = 8 + 8
umis assigned: [2, 10, 49, 83, 115, 134, 137, 153, 156]
of which 8 are surviving nonsolos
reads assigned: 2281
start codons at 47, 351, 376, 381, 393, 567
confident = true

TIG 2[bases=565]
0-73 ==> 7-80 on |123|IGHV3-30|5'UTR| [len=80] (mis=0)
73-424 ==> 0-351 on |124|IGHV3-30|L-REGION+V-REGION| [len=351] (mis=0)
443-494 ==> 0-51 on |57|IGHJ5|J-REGION| [len=51] (mis=4)
494-565 ==> 0-71 on |60|IGHM|C-REGION| [len=1358] (mis=0)
junction support: 13 umis using 237 reads
cdr3 = CAKVSTREAWGWFDPW at 415, score = 9 + 7
umis assigned: [0, 9, 15, 18, 20, 36, 39, 41, 44, 59] and 9 others
of which 19 are surviving nonsolos
reads assigned: 3603
start codons at 73, 224, 229, 287, 290, 308, 376
confident = true

TIG 3[bases=568]
0-44 ==> 8-52 on |282|IGKV3-20|5'UTR| [len=52] (mis=0)
44-392 ==> 0-348 on |283|IGKV3-20|L-REGION+V-REGION| [len=348] (mis=1)
394-432 ==> 0-38 on |217|IGKJ5|J-REGION| [len=38] (mis=0)
432-568 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 4 umis using 183 reads
cdr3 = CQQYGSSLPITF at 368, score = 9 + 8
umis assigned: [80, 85, 116, 154]
of which 4 are surviving nonsolos
reads assigned: 1176
start codons at 44, 252, 378, 474
confident = true
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 74.128 = GGAGCAAAGCTAGTTC-1

using 187 reads

====================================================================================

graph has 74 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 4[2^2, 5, 175]
surviving nonsolo ucounts = 1[175]
ids = [1]

====================================================================================

UMI info for barcode GGAGCAAAGCTAGTTC-1 contig 1 = GGGGTCACAA...
umi AGCGTCGAAG = 164 reads: +391 validated

GOOD CONTIGS

TIG 1[bases=570]
0-38 ==> 124-162 on |342|IGLV2-23|5'UTR| [len=162] (mis=0)
38-331 ==> 0-293 on |343|IGLV2-23|L-REGION+V-REGION| [len=340] (mis=22)
391-429 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=7)
429-570 ==> 0-141 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 22 reads
cdr3 = CSSYAGRTTFDVF at 362, score = 7 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 161
start codons at 38, 174, 177, 192, 195, 372
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 74.133 = GGAGCAAAGGATGGAA-1

using 537 reads

====================================================================================

graph has 198 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 4[2^2, 178, 352]
surviving nonsolo ucounts = 2[178, 352]
ids = [3, 6]

====================================================================================

UMI info for barcode GGAGCAAAGGATGGAA-1 contig 1 = GGAGGAACTG...
umi TAATACCTTC = 177 reads: +382 validated
umi TTCGTAACTG = 356 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=552]
0-34 ==> 13-47 on |278|IGKV3-11|5'UTR| [len=47] (mis=0)
34-379 ==> 0-345 on |279|IGKV3-11|L-REGION+V-REGION| [len=345] (mis=3)
379-416 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=0)
416-552 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 2 umis using 90 reads
cdr3 = CQQRSNWPLTF at 355, score = 9 + 9
umis assigned: [3, 6]
of which 2 are surviving nonsolos
reads assigned: 522
start codons at 34, 242, 458
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 74.134 = GGAGCAAAGGCATGTG-1

using 31 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 6[2^3, 3, 4, 15]
surviving nonsolo ucounts = 1[15]
ids = [3]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 74.138 = GGAGCAAAGGCTAGAC-1

using 1145 reads

====================================================================================

graph has 1582 edges initially, 10 edges after simplification

total ucounts = 557
nonsolo ucounts = 210[2^103, 3^38, 4^19, 5^13, 6^8, 7^8, 8^6, 9^4, 10^2, 11^2, 12, 13^2, 14^2, 20^2]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 74.165 = GGAGCAACACAGACTT-1

using 810 reads

====================================================================================

graph has 311 edges initially, 14 edges after simplification

total ucounts = 7
nonsolo ucounts = 3[171, 307, 328]
surviving nonsolo ucounts = 3[171, 307, 328]
ids = [2, 5, 6]

====================================================================================

UMI info for barcode GGAGCAACACAGACTT-1 contig 1 = GAGAAGAGCT...
umi GTTTAACGAC = 304 reads: +385 validated

UMI info for barcode GGAGCAACACAGACTT-1 contig 2 = GGAGTCAGTC...
umi TATGAATATC = 320 reads: +388 validated

UMI info for barcode GGAGCAACACAGACTT-1 contig 3 = ACCCAAAAAC...
umi CAAAGGGAGT = 161 reads: +436 validated

GOOD CONTIGS

TIG 1[bases=556]
0-35 ==> 17-52 on |282|IGKV3-20|5'UTR| [len=52] (mis=0)
35-383 ==> 0-348 on |283|IGKV3-20|L-REGION+V-REGION| [len=348] (mis=17)
389-420 ==> 8-39 on |214|IGKJ2|J-REGION| [len=39] (mis=2)
420-556 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 48 reads
cdr3 = CQHYGSSPLTF at 359, score = 9 + 8
umis assigned: [5]
of which 1 are surviving nonsolos
reads assigned: 300
start codons at 35, 243, 369, 462
confident = false

TIG 2[bases=550]
0-26 ==> 21-47 on |230|IGKV1-39|5'UTR| [len=47] (mis=0)
26-377 ==> 0-351 on |231|IGKV1-39|L-REGION+V-REGION| [len=351] (mis=11)
376-414 ==> 0-38 on |217|IGKJ5|J-REGION| [len=38] (mis=0)
414-550 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 42 reads
cdr3 = CQQSYRRPITF at 353, score = 8 + 8
umis assigned: [6]
of which 1 are surviving nonsolos
reads assigned: 318
start codons at 26, 32, 88, 101, 237, 336, 456
confident = false

TIG 3[bases=521]
0-54 ==> 5-59 on |64|IGHV1-18|5'UTR| [len=59] (mis=0)
54-407 ==> 0-353 on |65|IGHV1-18|L-REGION+V-REGION| [len=353] (mis=19)
442-490 ==> 0-48 on |55|IGHJ4|J-REGION| [len=48] (mis=6)
490-521 ==> 0-31 on |1|IGHA1|C-REGION| [len=1058] (mis=0)
junction support: 1 umis using 18 reads
cdr3 = CARDKYYRDPYYHDSGAVDYW at 396, score = 8 + 7
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 155
start codons at 54, 205, 252, 257, 274, 289, 318, 351, 433
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 74.171 = GGAGCAACACGCTTTC-1

using 341 reads

====================================================================================

graph has 112 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 2[3, 334]
surviving nonsolo ucounts = 1[334]
ids = [1]

====================================================================================

REJECT CONTIGS

TIG 1[bases=553]
0-33 ==> 14-47 on |278|IGKV3-11|5'UTR| [len=47] (mis=0)
0-33 ==> 5967-6000 on rc of segment after IGKV3-11 exon 1 [len=6000] (mis=0)
6-58 ==> 5709-5761 on segment before IGKV3OR2-5 exon 1 [len=6000] (mis=3)
33-378 ==> 0-345 on |279|IGKV3-11|L-REGION+V-REGION| [len=345] (mis=0)
379-417 ==> 0-38 on |217|IGKJ5|J-REGION| [len=38] (mis=1)
417-553 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 331
start codons at 33, 238, 241, 459
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 74.172 = GGAGCAACACGTGAGA-1

using 152 reads

====================================================================================

graph has 52 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[152]
surviving nonsolo ucounts = 1[152]
ids = [0]

====================================================================================

REJECT CONTIGS

TIG 1[bases=500]
0-80 ==> 0-80 on |125|IGHV3-33|5'UTR| [len=80] (mis=1)
49-116 ==> 6507-6574 on segment before IGHV3OR16-9 exon 1 [len=6692] (mis=2)
80-431 ==> 0-351 on |126|IGHV3-33|L-REGION+V-REGION| [len=351] (mis=2)
444-500 ==> 0-56 on |737|IGHJ6|J-REGION| [len=63] (mis=2)
cdr3 = CARDLRPYNYYYYGMDVW at 422, score = 9 + 7
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 125
start codons at 80, 231, 236, 289, 294, 297, 315, 383, 464
confident = false
VJ delta = 21
not full
not full
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 74.174 = GGAGCAACACTGCCAG-1

using 507 reads

====================================================================================

graph has 160 edges initially, 4 edges after simplification

total ucounts = 6
nonsolo ucounts = 5[2, 4, 10, 187, 303]
surviving nonsolo ucounts = 2[187, 303]
ids = [1, 2]

====================================================================================

UMI info for barcode GGAGCAACACTGCCAG-1 contig 1 = GCTCTGCTTC...
umi CGAAAGGCTG = 173 reads: +391 validated

UMI info for barcode GGAGCAACACTGCCAG-1 contig 2 = GTCAGACCCA...
umi GCCTTACGCA = 267 reads: +385 validated
umi TGATTCCGCT = 3 reads: -111 +29 -1X +6 -1X +1 -1X +4 -2X +2 -4X +2 -1X +1 -87X +27 -1X +6 -1X +20 -77X invalidated

GOOD CONTIGS

TIG 1[bases=555]
0-51 ==> 0-51 on |320|IGLV1-40|5'UTR| [len=51] (mis=0)
51-407 ==> 0-356 on |321|IGLV1-40|L-REGION+V-REGION| [len=356] (mis=12)
423-442 ==> 19-38 on |309|IGLJ1|J-REGION| [len=38] (mis=1)
442-555 ==> 0-113 on |305|IGLC1|C-REGION| [len=317] (mis=1)
junction support: 1 umis using 32 reads
cdr3 = CQSYDSSLSGYVF at 375, score = 8 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 170
start codons at 51, 205, 259, 358, 385, 406
confident = false

TIG 2[bases=490]
0-23 ==> 158-181 on |232|IGKV1-5|5'UTR| [len=181] (mis=0)
23-374 ==> 0-351 on |233|IGKV1-5|L-REGION+V-REGION| [len=351] (mis=2)
370-408 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
408-490 ==> 0-82 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 47 reads
cdr3 = CQQYNSYWTF at 350, score = 8 + 8
umis assigned: [2, 5]
of which 1 are surviving nonsolos
reads assigned: 268
start codons at 23, 29, 85, 98, 330, 450
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 74.175 = GGAGCAACACTTGGAT-1

using 242 reads

====================================================================================

graph has 100 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 2[2, 235]
surviving nonsolo ucounts = 1[235]
ids = [3]

====================================================================================

UMI info for barcode GGAGCAACACTTGGAT-1 contig 1 = ACCCAAAAAC...
umi CTAGCGGGAG = 228 reads: +436 validated

GOOD CONTIGS

TIG 1[bases=595]
0-54 ==> 5-59 on |64|IGHV1-18|5'UTR| [len=59] (mis=1)
54-407 ==> 0-353 on |65|IGHV1-18|L-REGION+V-REGION| [len=353] (mis=22)
456-490 ==> 12-46 on |54|IGHJ4|J-REGION| [len=46] (mis=0)
490-595 ==> 0-105 on |1|IGHA1|C-REGION| [len=1058] (mis=1)
junction support: 1 umis using 23 reads
cdr3 = CARDKHFRDPYYHYSGALDHW at 396, score = 7 + 7
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 224
start codons at 54, 252, 257, 274, 318, 351, 544
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 74.187 = GGAGCAACATCTCCCA-1

using 35 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[2, 32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 74.195 = GGAGCAAGTATTCGTG-1

using 303 reads

====================================================================================

graph has 99 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[302]
surviving nonsolo ucounts = 1[302]
ids = [0]

====================================================================================

UMI info for barcode GGAGCAAGTATTCGTG-1 contig 1 = GCTGGGGTCT...
umi GATGAGCTCA = 303 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=640]
41-402 ==> 0-361 on |339|IGLV2-14|L-REGION+V-REGION| [len=361] (mis=11)
391-429 ==> 0-38 on |309|IGLJ1|J-REGION| [len=38] (mis=3)
429-640 ==> 0-211 on |305|IGLC1|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 45 reads
cdr3 = CSSYTSSSTLVF at 365, score = 8 + 9
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 298
start codons at 41, 198, 242, 249, 252, 561
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 74.203 = GGAGCAAGTCTTGCGG-1

using 226 reads

====================================================================================

graph has 104 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 2[5, 218]
surviving nonsolo ucounts = 1[218]
ids = [1]

====================================================================================

UMI info for barcode GGAGCAAGTCTTGCGG-1 contig 1 = AGCTCTGGGA...
umi AAGTGCCTTA = 213 reads: +430 validated

GOOD CONTIGS

TIG 1[bases=559]
0-79 ==> 1-80 on |123|IGHV3-30|5'UTR| [len=80] (mis=2)
79-430 ==> 0-351 on |124|IGHV3-30|L-REGION+V-REGION| [len=351] (mis=15)
458-509 ==> 0-51 on |57|IGHJ5|J-REGION| [len=51] (mis=1)
509-559 ==> 0-50 on |1|IGHA1|C-REGION| [len=1058] (mis=0)
junction support: 1 umis using 31 reads
cdr3 = CARGAGYDFLSGHHWFDPW at 421, score = 8 + 7
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 208
start codons at 79, 235, 286, 293, 296, 314, 382
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 74.209 = GGAGCAAGTGGGTATG-1

using 311 reads

====================================================================================

graph has 78 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[10, 299]
surviving nonsolo ucounts = 1[299]
ids = [2]

====================================================================================

UMI info for barcode GGAGCAAGTGGGTATG-1 contig 1 = GGAATCAGTC...
umi TGAGGTTGAC = 296 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=550]
0-26 ==> 1-27 on |222|IGKV1-17|5'UTR| [len=27] (mis=0)
26-377 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
376-414 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
414-550 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 38 reads
cdr3 = CLQYSSSPWTF at 353, score = 9 + 8
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 294
start codons at 26, 32, 101, 237, 456
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 74.211 = GGAGCAAGTTATGTGC-1

using 288 reads

====================================================================================

graph has 60 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[287]
surviving nonsolo ucounts = 1[287]
ids = [0]

====================================================================================

UMI info for barcode GGAGCAAGTTATGTGC-1 contig 1 = ATCATCCAAC...
umi TCACACCTCA = 266 reads: +430 validated

GOOD CONTIGS

TIG 1[bases=546]
0-58 ==> 246-304 on |78|IGHV1-46|5'UTR| [len=304] (mis=1)
58-411 ==> 0-353 on |79|IGHV1-46|L-REGION+V-REGION| [len=353] (mis=20)
440-488 ==> 13-61 on |58|IGHJ6|J-REGION| [len=61] (mis=1)
488-546 ==> 0-58 on |1|IGHA1|C-REGION| [len=1058] (mis=0)
junction support: 1 umis using 25 reads
cdr3 = CARGDRRAAAFYYHYMDVW at 400, score = 8 + 7
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 265
start codons at 58, 214, 256, 322, 355, 445, 542
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 74.213 = GGAGCAAGTTCTCATT-1

using 557 reads

====================================================================================

graph has 206 edges initially, 48 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[198, 358]
surviving nonsolo ucounts = 2[198, 358]
ids = [0, 1]

====================================================================================

UMI info for barcode GGAGCAAGTTCTCATT-1 contig 1 = AGTCCCAACC...
umi GAATAGGACC = 357 reads: +391 validated

UMI info for barcode GGAGCAAGTTCTCATT-1 contig 2 = GGAGTCAGAC...
umi ACATCATGCC = 199 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=547]
0-20 ==> 11-31 on |226|IGKV1-33|5'UTR| [len=31] (mis=0)
20-371 ==> 0-351 on |227|IGKV1-33|L-REGION+V-REGION| [len=351] (mis=1)
374-411 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=0)
411-547 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 45 reads
cdr3 = CQQYDNLPLLTF at 347, score = 9 + 9
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 354
start codons at 20, 26, 82, 95, 234, 357, 453
confident = false

TIG 2[bases=550]
0-26 ==> 155-181 on |232|IGKV1-5|5'UTR| [len=181] (mis=1)
26-377 ==> 0-351 on |233|IGKV1-5|L-REGION+V-REGION| [len=351] (mis=17)
377-414 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=3)
414-550 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 20 reads
cdr3 = CQQYNSYALSF at 353, score = 8 + 7
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 196
start codons at 26, 32, 88, 101, 237, 240, 333, 372, 456
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 74.216 = GGAGCAATCAAACAAG-1

using 69 reads

====================================================================================

graph has 80 edges initially, 4 edges after simplification

total ucounts = 17
nonsolo ucounts = 12[2^2, 3^3, 5^2, 7, 8^3, 10]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 74.222 = GGAGCAATCATATCGG-1

using 12 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[2, 8]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 74.227 = GGAGCAATCCAGTAGT-1

using 1704 reads

====================================================================================

graph has 2255 edges initially, 22 edges after simplification

total ucounts = 702
nonsolo ucounts = 301[2^122, 3^74, 4^42, 5^18, 6^16, 7^6, 8^6, 9^6, 10^4, 11, 12^2, 13, 16, 19, 216]
surviving nonsolo ucounts = 1[216]
ids = [176]

====================================================================================

UMI info for barcode GGAGCAATCCAGTAGT-1 contig 1 = GCTACAACAG...
umi CACACCTCTG = 215 reads: +403 validated

GOOD CONTIGS

TIG 1[bases=567]
0-28 ==> 147-175 on |295|IGKV4-1|5'UTR| [len=175] (mis=0)
28-391 ==> 0-363 on |296|IGKV4-1|L-REGION+V-REGION| [len=363] (mis=1)
393-431 ==> 0-38 on |215|IGKJ3|J-REGION| [len=38] (mis=0)
431-567 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 27 reads
cdr3 = CQQYYSTLPFTF at 367, score = 9 + 8
umis assigned: [176]
of which 1 are surviving nonsolos
reads assigned: 214
start codons at 28, 97, 350, 473
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 74.229 = GGAGCAATCCGTCATC-1

using 244 reads

====================================================================================

graph has 102 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 1[236]
surviving nonsolo ucounts = 1[236]
ids = [0]

====================================================================================

UMI info for barcode GGAGCAATCCGTCATC-1 contig 1 = GTGGGCTCAG...
umi ATAGATGAAG = 240 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=628]
0-35 ==> 0-35 on |350|IGLV3-10|5'UTR| [len=35] (mis=0)
35-382 ==> 0-347 on |351|IGLV3-10|L-REGION+V-REGION| [len=347] (mis=1)
379-417 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=0)
417-628 ==> 0-211 on |307|IGLC3|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 44 reads
cdr3 = CYSTDSSGNHWVF at 350, score = 7 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 235
start codons at 35, 96, 165, 183, 234, 296, 333
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 74.231 = GGAGCAATCCTCAACC-1

using 233 reads

====================================================================================

graph has 124 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 6[2^3, 7, 9, 209]
surviving nonsolo ucounts = 1[209]
ids = [3]

====================================================================================

UMI info for barcode GGAGCAATCCTCAACC-1 contig 1 = GGGGGCTCCA...
umi CACACTCCAC = 191 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=590]
46-398 ==> 0-352 on |333|IGLV10-54|L-REGION+V-REGION| [len=352] (mis=19)
396-434 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=1)
434-590 ==> 0-156 on |307|IGLC3|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 29 reads
cdr3 = CSAWDSSLNVWVF at 367, score = 7 + 8
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 187
start codons at 46, 185, 375, 392
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 74.235 = GGAGCAATCGCTGATA-1

using 623 reads

====================================================================================

graph has 290 edges initially, 4 edges after simplification

total ucounts = 9
nonsolo ucounts = 3[13, 208, 396]
surviving nonsolo ucounts = 2[208, 396]
ids = [0, 1]

====================================================================================

UMI info for barcode GGAGCAATCGCTGATA-1 contig 1 = AATCAGTCCC...
umi ATATCAAGGC = 399 reads: +388 validated

UMI info for barcode GGAGCAATCGCTGATA-1 contig 2 = TGGGGACCCA...
umi AGATCGTTTC = 204 reads: +436 validated

GOOD CONTIGS

TIG 1[bases=548]
0-24 ==> 3-27 on |222|IGKV1-17|5'UTR| [len=27] (mis=0)
24-375 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
374-412 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
412-548 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 53 reads
cdr3 = CLQYSSSPWTF at 351, score = 9 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 387
start codons at 24, 30, 99, 235, 454
confident = false

TIG 2[bases=593]
0-59 ==> 0-59 on |64|IGHV1-18|5'UTR| [len=59] (mis=6)
59-412 ==> 0-353 on |65|IGHV1-18|L-REGION+V-REGION| [len=353] (mis=22)
461-495 ==> 12-46 on |54|IGHJ4|J-REGION| [len=46] (mis=0)
495-593 ==> 0-98 on |1|IGHA1|C-REGION| [len=1058] (mis=0)
junction support: 1 umis using 17 reads
cdr3 = CARDKHFRDPYYHYSGALDHW at 401, score = 7 + 7
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 196
start codons at 59, 257, 262, 279, 323, 356, 549
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 74.244 = GGATGTTAGAATTGTG-1

using 261 reads

====================================================================================

graph has 72 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[261]
surviving nonsolo ucounts = 1[261]
ids = [0]

====================================================================================

UMI info for barcode GGATGTTAGAATTGTG-1 contig 1 = GAGGAGCCCA...
umi AACTTGAGTT = 243 reads: +430 validated

GOOD CONTIGS

TIG 1[bases=499]
0-69 ==> 11-80 on |123|IGHV3-30|5'UTR| [len=80] (mis=2)
69-420 ==> 0-351 on |124|IGHV3-30|L-REGION+V-REGION| [len=351] (mis=15)
448-499 ==> 0-51 on |57|IGHJ5|J-REGION| [len=51] (mis=1)
junction support: 1 umis using 23 reads
cdr3 = CARGAGYDFLSGHHWFDPW at 411, score = 8 + 7
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 241
start codons at 69, 225, 276, 283, 286, 304, 372
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 74.245 = GGATGTTAGACAGACC-1

using 2644 reads

====================================================================================

graph has 504 edges initially, 2 edges after simplification

total ucounts = 12
nonsolo ucounts = 5[2, 3, 7, 930, 1695]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 74.251 = GGATGTTAGAGTCGGT-1

using 347 reads

====================================================================================

graph has 124 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 2[4, 340]
surviving nonsolo ucounts = 1[340]
ids = [0]

====================================================================================

UMI info for barcode GGATGTTAGAGTCGGT-1 contig 1 = GAAGAGCTGC...
umi AATAAATAGG = 299 reads: +385 validated

GOOD CONTIGS

TIG 1[bases=488]
0-33 ==> 19-52 on |282|IGKV3-20|5'UTR| [len=52] (mis=0)
33-381 ==> 0-348 on |283|IGKV3-20|L-REGION+V-REGION| [len=348] (mis=19)
380-418 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
418-488 ==> 0-70 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 53 reads
cdr3 = CQQYGSSPWTF at 357, score = 8 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 290
start codons at 33, 367, 460
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 74.257 = GGATGTTAGCCACCTG-1

using 1106 reads

====================================================================================

graph has 486 edges initially, 12 edges after simplification

total ucounts = 44
nonsolo ucounts = 27[2^6, 3^5, 4, 5^2, 6, 7, 8^3, 11, 12, 13, 15, 20, 288, 312, 340]
surviving nonsolo ucounts = 3[288, 312, 340]
ids = [6, 34, 24]

====================================================================================

UMI info for barcode GGATGTTAGCCACCTG-1 contig 1 = ACAAGAGGCA...
umi ATCGGTCCCT = 269 reads: +394 validated

UMI info for barcode GGATGTTAGCCACCTG-1 contig 2 = GAGAAGAGCT...
umi TCGGTCCGAG = 309 reads: +385 validated

UMI info for barcode GGATGTTAGCCACCTG-1 contig 3 = GGGAATCAGT...
umi GACGCCATCC = 334 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=584]
0-31 ==> 20-51 on |320|IGLV1-40|5'UTR| [len=51] (mis=0)
31-387 ==> 0-356 on |321|IGLV1-40|L-REGION+V-REGION| [len=356] (mis=13)
387-425 ==> 0-38 on |309|IGLJ1|J-REGION| [len=38] (mis=1)
425-584 ==> 0-159 on |305|IGLC1|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 59 reads
cdr3 = CQSYDSSLSGLYVF at 355, score = 7 + 8
umis assigned: [6]
of which 1 are surviving nonsolos
reads assigned: 263
start codons at 31, 185, 188, 239, 338, 365, 389, 557
confident = false

TIG 2[bases=556]
0-35 ==> 17-52 on |282|IGKV3-20|5'UTR| [len=52] (mis=0)
35-383 ==> 0-348 on |283|IGKV3-20|L-REGION+V-REGION| [len=348] (mis=6)
382-420 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=2)
420-556 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 47 reads
cdr3 = CQQYGSSLGTF at 359, score = 9 + 8
umis assigned: [34]
of which 1 are surviving nonsolos
reads assigned: 306
start codons at 35, 243, 369, 462
confident = false

TIG 3[bases=551]
0-27 ==> 0-27 on |222|IGKV1-17|5'UTR| [len=27] (mis=1)
27-378 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
377-415 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
415-551 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 58 reads
cdr3 = CLQYSSSPWTF at 354, score = 9 + 8
umis assigned: [24]
of which 1 are surviving nonsolos
reads assigned: 328
start codons at 27, 33, 102, 238, 457
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 74.259 = GGATGTTAGCCAGGAT-1

using 258 reads

====================================================================================

graph has 122 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[2, 255]
surviving nonsolo ucounts = 1[255]
ids = [1]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 74.260 = GGATGTTAGCGATATA-1

using 441 reads

====================================================================================

graph has 192 edges initially, 4 edges after simplification

total ucounts = 8
nonsolo ucounts = 4[2^2, 77, 356]
surviving nonsolo ucounts = 2[77, 356]
ids = [6, 4]

====================================================================================

UMI info for barcode GGATGTTAGCGATATA-1 contig 1 = AGGAACTGCT...
umi CTTGCCTGCA = 355 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=556]
0-32 ==> 65-97 on |280|IGKV3-15|5'UTR| [len=97] (mis=0)
32-377 ==> 0-345 on |281|IGKV3-15|L-REGION+V-REGION| [len=345] (mis=6)
381-420 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=4)
420-556 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 54 reads
cdr3 = CQQYNNWPPMYTF at 353, score = 8 + 8
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 350
start codons at 32, 101, 237, 380, 462
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 74.273 = GGATGTTAGGGATCTG-1

using 241 reads

====================================================================================

graph has 70 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 1[236]
surviving nonsolo ucounts = 1[236]
ids = [4]

====================================================================================

UMI info for barcode GGATGTTAGGGATCTG-1 contig 1 = GCTGTGCTGT...
umi TTAATACCAG = 217 reads: +385 validated

GOOD CONTIGS

TIG 1[bases=506]
0-43 ==> 0-43 on |362|IGLV3-25|5'UTR| [len=43] (mis=0)
43-380 ==> 0-337 on |363|IGLV3-25|L-REGION+V-REGION| [len=337] (mis=0)
390-428 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=0)
428-506 ==> 0-78 on |307|IGLC3|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 37 reads
cdr3 = CQSADSSGTYSWVF at 358, score = 8 + 8
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 216
start codons at 43, 104, 173, 191
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 74.276 = GGATGTTAGTCCGTAT-1

using 317 reads

====================================================================================

graph has 107 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[5, 311]
surviving nonsolo ucounts = 1[311]
ids = [2]

====================================================================================

UMI info for barcode GGATGTTAGTCCGTAT-1 contig 1 = TGGGCCTCAG...
umi TTTGCTAAGT = 283 reads: +373 validated

GOOD CONTIGS

TIG 1[bases=563]
0-36 ==> 16-52 on |348|IGLV3-1|5'UTR| [len=52] (mis=0)
36-372 ==> 0-336 on |349|IGLV3-1|L-REGION+V-REGION| [len=346] (mis=1)
371-409 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=1)
409-563 ==> 0-154 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 54 reads
cdr3 = CQAWDSSVVF at 351, score = 6 + 8
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 276
start codons at 36, 41, 97, 184, 330, 334
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 74.278 = GGATGTTAGTGCCATT-1

using 521 reads

====================================================================================

graph has 192 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[2, 5, 512]
surviving nonsolo ucounts = 1[512]
ids = [2]

====================================================================================

UMI info for barcode GGATGTTAGTGCCATT-1 contig 1 = TGGGGGGACT...
umi TAAGTCATCG = 505 reads: +415 validated

GOOD CONTIGS

TIG 1[bases=509]
23-381 ==> 0-358 on |98|IGHV2-5|L-REGION+V-REGION| [len=358] (mis=9)
390-438 ==> 0-48 on |55|IGHJ4|J-REGION| [len=48] (mis=2)
438-509 ==> 0-71 on |60|IGHM|C-REGION| [len=1358] (mis=0)
junction support: 1 umis using 38 reads
cdr3 = CAHCTSIDYLVYW at 368, score = 7 + 7
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 499
start codons at 23, 67, 246, 249, 329, 338
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 74.281 = GGATGTTAGTGTCTCA-1

using 522 reads

====================================================================================

graph has 168 edges initially, 6 edges after simplification

total ucounts = 8
nonsolo ucounts = 2[216, 300]
surviving nonsolo ucounts = 2[216, 300]
ids = [5, 0]

====================================================================================

UMI info for barcode GGATGTTAGTGTCTCA-1 contig 1 = GGGTCAGAGC...
umi TCCAGGCGAA = 215 reads: +385 validated

UMI info for barcode GGATGTTAGTGTCTCA-1 contig 2 = TGGGGAGGAA...
umi AAAATATCTG = 300 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=570]
0-49 ==> 48-97 on |280|IGKV3-15|5'UTR| [len=97] (mis=0)
49-394 ==> 0-345 on |281|IGKV3-15|L-REGION+V-REGION| [len=345] (mis=0)
396-434 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
434-570 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 21 reads
cdr3 = CQQYNNWPPWTF at 370, score = 9 + 8
umis assigned: [5]
of which 1 are surviving nonsolos
reads assigned: 213
start codons at 49, 118, 254, 476
confident = false

TIG 2[bases=556]
32-383 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
382-420 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
420-556 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 40 reads
cdr3 = CLQYSSSPWTF at 359, score = 9 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 297
start codons at 32, 38, 107, 243, 462
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 74.283 = GGATGTTAGTTGAGTA-1

using 352 reads

====================================================================================

graph has 144 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[351]
surviving nonsolo ucounts = 1[351]
ids = [0]

====================================================================================

UMI info for barcode GGATGTTAGTTGAGTA-1 contig 1 = GAGGAATCAG...
umi ACTGATCTTG = 355 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=552]
28-379 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
378-416 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
416-552 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 52 reads
cdr3 = CLQYSSSPWTF at 355, score = 9 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 347
start codons at 28, 34, 103, 239, 458
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 74.288 = GGATGTTCAACAACCT-1

using 261 reads

====================================================================================

graph has 108 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 1[253]
surviving nonsolo ucounts = 1[253]
ids = [1]

====================================================================================

UMI info for barcode GGATGTTCAACAACCT-1 contig 1 = CTGGGAGAGG...
umi ATCACTCAGT = 247 reads: +445 validated

GOOD CONTIGS

TIG 1[bases=535]
75-428 ==> 0-353 on |128|IGHV3-33|L-REGION+V-REGION| [len=353] (mis=15)
474-520 ==> 17-63 on |737|IGHJ6|J-REGION| [len=63] (mis=2)
junction support: 1 umis using 17 reads
cdr3 = CARDAPYMIGGGTAQWVPMGMDVW at 417, score = 9 + 7
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 245
start codons at 75, 226, 231, 289, 292, 301, 378, 427, 438, 461, 471, 477, 501
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 74.293 = GGATGTTCAATGGTCT-1

using 627 reads

====================================================================================

graph has 198 edges initially, 4 edges after simplification

total ucounts = 5
nonsolo ucounts = 2[246, 378]
surviving nonsolo ucounts = 2[246, 378]
ids = [0, 1]

====================================================================================

UMI info for barcode GGATGTTCAATGGTCT-1 contig 1 = AGCTTCAGCT...
umi CAGCAGCTGT = 236 reads: +388 validated

UMI info for barcode GGATGTTCAATGGTCT-1 contig 2 = TGGGGAGGAG...
umi CGAGCACTCA = 379 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=544]
0-47 ==> 67-114 on |322|IGLV1-44|5'UTR| [len=114] (mis=0)
47-400 ==> 0-353 on |323|IGLV1-44|L-REGION+V-REGION| [len=353] (mis=2)
397-435 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=0)
435-544 ==> 0-109 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 39 reads
cdr3 = CAAWDDSLNGVVF at 368, score = 8 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 233
start codons at 47, 351, 376, 381, 393
confident = false

TIG 2[bases=556]
32-385 ==> 0-353 on |253|IGKV1D-39|L-REGION+V-REGION| [len=353] (mis=14)
383-420 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=0)
420-556 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 58 reads
cdr3 = CQQSYTTLLTF at 359, score = 9 + 9
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 372
start codons at 32, 38, 107, 243, 462
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 74.295 = GGATGTTCACAGCGTC-1

using 500 reads

====================================================================================

graph has 194 edges initially, 4 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[4, 7, 177, 310]
surviving nonsolo ucounts = 2[177, 310]
ids = [0, 3]

====================================================================================

UMI info for barcode GGATGTTCACAGCGTC-1 contig 1 = CTGGGCCTAA...
umi AACGGCGAGC = 152 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=524]
0-37 ==> 214-251 on |358|IGLV3-21|5'UTR| [len=251] (mis=0)
37-382 ==> 0-345 on |359|IGLV3-21|L-REGION+V-REGION| [len=351] (mis=30)
381-419 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=1)
419-524 ==> 0-105 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 25 reads
cdr3 = CQVWYSNSDHVVF at 352, score = 7 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 149
start codons at 37, 232, 239, 335, 380
confident = false

REJECT CONTIGS

TIG 1[bases=306]
0-152 ==> 201-353 on |88|IGHV1-69D|L-REGION+V-REGION| [len=353] (mis=0)
185-235 ==> 0-50 on |53|IGHJ3|J-REGION| [len=50] (mis=1)
235-306 ==> 0-71 on |60|IGHM|C-REGION| [len=1358] (mis=0)
cdr3 = CARDSLVEYYGSGSYYAFDIW at 141, score = 9 + 8
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 302
start codons at 96, 169, 187, 216
confident = false
VJ delta = 10
not full
not full
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 74.299 = GGATGTTCACGGCTAC-1

using 38 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 5[3^3, 10, 18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 74.307 = GGATGTTCAGATCGGA-1

using 262 reads

====================================================================================

graph has 58 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[262]
surviving nonsolo ucounts = 1[262]
ids = [0]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 74.309 = GGATGTTCAGCCTATA-1

using 549 reads

====================================================================================

graph has 232 edges initially, 4 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[266, 281]
surviving nonsolo ucounts = 1[281]
ids = [2]

====================================================================================

UMI info for barcode GGATGTTCAGCCTATA-1 contig 1 = AGACCCAGTC...
umi GATTCATGTC = 35 reads: -246X +8 -1XX +8 -1XX +11 -1XX +27 -1XX +4 -1XX +9 -2XX +9 -1XX +2 -1XX +6 -1 +1 -2XX +1 -3XX +2 -1XX +1 -2XX +6 -1XX +3 -3XX +9 -1XX +12 invalidated
umi GCTAACTTGC = 229 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=483]
0-20 ==> 161-181 on |232|IGKV1-5|5'UTR| [len=181] (mis=0)
20-371 ==> 0-351 on |233|IGKV1-5|L-REGION+V-REGION| [len=351] (mis=4)
369-408 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=2)
408-483 ==> 0-75 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 44 reads
cdr3 = CQQYNSYLYTF at 347, score = 8 + 8
umis assigned: [1, 2]
of which 1 are surviving nonsolos
reads assigned: 262
start codons at 20, 26, 82, 95, 327, 450
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 74.311 = GGATGTTCAGGTCCAC-1

using 646 reads

====================================================================================

graph has 268 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 1[640]
surviving nonsolo ucounts = 1[640]
ids = [1]

====================================================================================

UMI info for barcode GGATGTTCAGGTCCAC-1 contig 1 = GGAGGAATCA...
umi AAATCCTTCG = 647 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=553]
29-380 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
379-417 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
417-553 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 114 reads
cdr3 = CLQYSSSPWTF at 356, score = 9 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 634
start codons at 29, 35, 104, 240, 459
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 74.313 = GGATGTTCAGTATAAG-1

using 2364 reads

====================================================================================

graph has 2937 edges initially, 14 edges after simplification

total ucounts = 848
nonsolo ucounts = 416[2^144, 3^97, 4^68, 5^32, 6^23, 7^12, 8^12, 9^9, 10^8, 11, 13^3, 14^2, 15, 17, 31, 62, 239]
surviving nonsolo ucounts = 1[239]
ids = [35]

====================================================================================

UMI info for barcode GGATGTTCAGTATAAG-1 contig 1 = GGGGGGGGTC...
umi ACGGTTTCTT = 231 reads: +391 validated

GOOD CONTIGS

TIG 1[bases=566]
42-364 ==> 0-322 on |339|IGLV2-14|L-REGION+V-REGION| [len=361] (mis=9)
395-433 ==> 0-38 on |309|IGLJ1|J-REGION| [len=38] (mis=1)
433-566 ==> 0-133 on |305|IGLC1|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 33 reads
cdr3 = CCSFTVNTRSYVF at 366, score = 7 + 8
umis assigned: [35]
of which 1 are surviving nonsolos
reads assigned: 227
start codons at 42, 199, 243, 253, 397
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 74.319 = GGATGTTCATGCCTAA-1

using 446 reads

====================================================================================

graph has 182 edges initially, 4 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[163, 283]
surviving nonsolo ucounts = 2[163, 283]
ids = [1, 0]

====================================================================================

UMI info for barcode GGATGTTCATGCCTAA-1 contig 1 = GGAACTGCTC...
umi CGTCCCTTGT = 145 reads: +385 validated

UMI info for barcode GGATGTTCATGCCTAA-1 contig 2 = GGACTCCTGT...
umi ACTCCTCGGA = 268 reads: +433 validated

GOOD CONTIGS

TIG 1[bases=512]
0-31 ==> 16-47 on |278|IGKV3-11|5'UTR| [len=47] (mis=0)
31-376 ==> 0-345 on |279|IGKV3-11|L-REGION+V-REGION| [len=345] (mis=6)
379-416 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=0)
416-512 ==> 0-96 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 17 reads
cdr3 = CQQRSNSQPLTF at 352, score = 9 + 9
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 139
start codons at 31, 236, 239, 458
confident = false

TIG 2[bases=581]
18-376 ==> 0-358 on |98|IGHV2-5|L-REGION+V-REGION| [len=358] (mis=19)
401-451 ==> 0-50 on |53|IGHJ3|J-REGION| [len=50] (mis=4)
451-581 ==> 0-130 on |40|IGHG1|C-REGION| [len=884] (mis=1)
junction support: 1 umis using 28 reads
cdr3 = CAHQLAIVTGYYENAFDIW at 363, score = 8 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 263
start codons at 18, 62, 241, 244, 247, 333, 403
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 74.321 = GGATGTTCATTAACCG-1

using 204 reads

====================================================================================

graph has 84 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 1[198]
surviving nonsolo ucounts = 1[198]
ids = [1]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 74.322 = GGATGTTCATTCACTT-1

using 766 reads

====================================================================================

graph has 216 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 2[2, 761]
surviving nonsolo ucounts = 1[761]
ids = [0]

====================================================================================

REJECT CONTIGS

TIG 1[bases=350]
6-177 ==> 174-345 on |281|IGKV3-15|L-REGION+V-REGION| [len=345] (mis=2)
177-214 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=0)
214-350 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
cdr3 = CQQYNNWPLTF at 153, score = 9 + 9
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 750
start codons at 37, 256
confident = false
not full
VJ delta = 13
not full
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 74.325 = GGATGTTGTACTCGCG-1

using 66 reads

====================================================================================

graph has 70 edges initially, 2 edges after simplification

total ucounts = 14
nonsolo ucounts = 10[2^2, 4, 5, 6^4, 9, 16]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 74.332 = GGATGTTGTCAAAGAT-1

using 139 reads

====================================================================================

graph has 48 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 1[136]
surviving nonsolo ucounts = 1[136]
ids = [3]

====================================================================================

UMI info for barcode GGATGTTGTCAAAGAT-1 contig 1 = GAATCAGTCC...
umi TGCTTTAGGT = 120 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=440]
0-25 ==> 2-27 on |222|IGKV1-17|5'UTR| [len=27] (mis=0)
25-376 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=6)
375-413 ==> 0-38 on |215|IGKJ3|J-REGION| [len=38] (mis=0)
413-440 ==> 0-27 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 20 reads
cdr3 = CLQHKSYPFTF at 352, score = 9 + 8
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 120
start codons at 25, 31, 100, 236
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 74.344 = GGATGTTGTCTTGCGG-1

using 297 reads

====================================================================================

graph has 194 edges initially, 2 edges after simplification

total ucounts = 21
nonsolo ucounts = 15[2^5, 4^2, 5, 6, 7, 8^3, 9, 222]
surviving nonsolo ucounts = 1[222]
ids = [8]

====================================================================================

UMI info for barcode GGATGTTGTCTTGCGG-1 contig 1 = AGAGCTCTGG...
umi CTCTACTTTA = 201 reads: +385 validated

GOOD CONTIGS

TIG 1[bases=477]
0-44 ==> 8-52 on |282|IGKV3-20|5'UTR| [len=52] (mis=0)
44-392 ==> 0-348 on |283|IGKV3-20|L-REGION+V-REGION| [len=348] (mis=0)
391-429 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=2)
429-477 ==> 0-48 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 23 reads
cdr3 = CQQYGSSPRTF at 368, score = 9 + 8
umis assigned: [8]
of which 1 are surviving nonsolos
reads assigned: 195
start codons at 44, 252, 378, 471
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 74.352 = GGATGTTGTTACCGAT-1

using 364 reads

====================================================================================

graph has 138 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 1[357]
surviving nonsolo ucounts = 1[357]
ids = [5]

====================================================================================

UMI info for barcode GGATGTTGTTACCGAT-1 contig 1 = GGGGAGAGTC...
umi GTTAACATAA = 353 reads: +439 validated

GOOD CONTIGS

TIG 1[bases=520]
10-381 ==> 0-371 on |186|IGHV4-34|L-REGION+V-REGION| [len=371] (mis=11)
403-449 ==> 0-46 on |54|IGHJ4|J-REGION| [len=46] (mis=4)
449-520 ==> 0-71 on |60|IGHM|C-REGION| [len=1358] (mis=0)
junction support: 1 umis using 35 reads
cdr3 = CARGAARSGTVTLDYW at 370, score = 9 + 7
umis assigned: [5]
of which 1 are surviving nonsolos
reads assigned: 348
start codons at 10, 31, 75, 161, 266
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 74.355 = GGATGTTGTTGGTAAA-1

using 68 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[68]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 74.358 = GGATGTTTCAACGGCC-1

using 166 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 1[162]
surviving nonsolo ucounts = 1[162]
ids = [1]

====================================================================================

UMI info for barcode GGATGTTTCAACGGCC-1 contig 1 = AGCATCATCC...
umi GAGTCCATAC = 161 reads: +427 validated

GOOD CONTIGS

TIG 1[bases=559]
0-61 ==> 243-304 on |78|IGHV1-46|5'UTR| [len=304] (mis=0)
61-414 ==> 0-353 on |79|IGHV1-46|L-REGION+V-REGION| [len=353] (mis=4)
425-488 ==> 0-63 on |59|IGHJ6|J-REGION| [len=63] (mis=4)
488-559 ==> 0-71 on |60|IGHM|C-REGION| [len=1358] (mis=0)
junction support: 1 umis using 16 reads
cdr3 = CARSVTTGPYYYYYMDVW at 403, score = 9 + 7
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 160
start codons at 61, 217, 259, 325, 358, 445
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 74.359 = GGATGTTTCACGGTTA-1

using 12 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[12]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 74.371 = GGATGTTTCCCACTTG-1

using 454 reads

====================================================================================

graph has 203 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 2[50, 399]
surviving nonsolo ucounts = 2[50, 399]
ids = [4, 0]

====================================================================================

UMI info for barcode GGATGTTTCCCACTTG-1 contig 1 = AGAGCTCTGG...
umi ACCCAATCGC = 400 reads: +382 validated
umi GTCATATGCC = 49 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=562]
0-44 ==> 53-97 on |280|IGKV3-15|5'UTR| [len=97] (mis=0)
44-389 ==> 0-345 on |281|IGKV3-15|L-REGION+V-REGION| [len=345] (mis=1)
388-426 ==> 0-38 on |217|IGKJ5|J-REGION| [len=38] (mis=2)
426-562 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 2 umis using 66 reads
cdr3 = CQQYNNWPLTF at 365, score = 9 + 9
umis assigned: [0, 4]
of which 2 are surviving nonsolos
reads assigned: 441
start codons at 44, 113, 249, 468
confident = false
NOT paired!

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BARCODE 74.378 = GGATGTTTCCTTGGTC-1

using 313 reads

====================================================================================

graph has 148 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[2, 7, 302]
surviving nonsolo ucounts = 1[302]
ids = [4]

====================================================================================

UMI info for barcode GGATGTTTCCTTGGTC-1 contig 1 = GAGCTACAAC...
umi TTCACATCAT = 279 reads: +400 validated

GOOD CONTIGS

TIG 1[bases=488]
0-30 ==> 145-175 on |295|IGKV4-1|5'UTR| [len=175] (mis=0)
30-393 ==> 0-363 on |296|IGKV4-1|L-REGION+V-REGION| [len=363] (mis=0)
392-430 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=2)
430-488 ==> 0-58 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 36 reads
cdr3 = CQQYYSTPRTF at 369, score = 9 + 8
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 270
start codons at 30, 99, 352, 472
confident = false
NOT paired!

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BARCODE 74.379 = GGATGTTTCGCCTGTT-1

using 61 reads

====================================================================================

graph has 48 edges initially, 4 edges after simplification

total ucounts = 14
nonsolo ucounts = 10[2^3, 3, 5, 6, 7, 8^2, 14]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!
sorting bam, mem = 0.04
running samtools sort -l 8G -o /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk074-ucc6a7a72ce/files/contig_bam_sorted.bam /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk074-ucc6a7a72ce/files/contig_bam.bam

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7.904 seconds used processing barcodes, peak mem = 0.23
