[stdout]

starting vdj_asm_asm, mem = 0.01
available mem = 832.66 GB
/scratch/etanis/software/cellranger-6.1.2/lib/bin/cr_vdj martian assembly main /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk060-ucc6a7a72ce /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk060-ucc6a7a72ce/files /scratch/etanis/ST-118/multi/dimitri-multi/journal/SC_MULTI_CS.SC_MULTI_CORE.MULTI_GEM_WELL_PROCESSOR.VDJ_B_GEM_WELL_PROCESSOR.SC_VDJ_CONTIG_ASSEMBLER.ASSEMBLE_VDJ.fork0.chnk060.ucc6a7a72ce
n50_n50_rpu = 288

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 60.0 = GACGTGCGTGTGAAAT-1

using 749 reads

====================================================================================

graph has 784 edges initially, 2 edges after simplification

total ucounts = 255
nonsolo ucounts = 92[2^38, 3^25, 4^10, 5^6, 6^5, 7, 8^2, 11, 12, 13, 14, 262]
surviving nonsolo ucounts = 1[262]
ids = [234]

====================================================================================

UMI info for barcode GACGTGCGTGTGAAAT-1 contig 1 = GAGTCAGACT...
umi TGGGGCTCCT = 251 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=490]
0-25 ==> 156-181 on |232|IGKV1-5|5'UTR| [len=181] (mis=1)
25-376 ==> 0-351 on |233|IGKV1-5|L-REGION+V-REGION| [len=351] (mis=7)
375-413 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=2)
413-490 ==> 0-77 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 36 reads
cdr3 = CQQYNSYPRTF at 352, score = 8 + 8
umis assigned: [234]
of which 1 are surviving nonsolos
reads assigned: 247
start codons at 25, 31, 87, 100, 236, 239, 332, 455
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 60.1 = GACGTGCGTGTGCCTG-1

using 136 reads

====================================================================================

graph has 48 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[135]
surviving nonsolo ucounts = 1[135]
ids = [1]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 60.5 = GACGTGCGTTCCCTTG-1

using 140 reads

====================================================================================

graph has 74 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[139]
surviving nonsolo ucounts = 1[139]
ids = [0]

====================================================================================

UMI info for barcode GACGTGCGTTCCCTTG-1 contig 1 = ACCCAAAAAC...
umi TTAATAAGCA = 135 reads: +409 validated

GOOD CONTIGS

TIG 1[bases=504]
0-54 ==> 5-59 on |64|IGHV1-18|5'UTR| [len=59] (mis=0)
54-407 ==> 0-353 on |65|IGHV1-18|L-REGION+V-REGION| [len=353] (mis=23)
412-463 ==> 0-51 on |57|IGHJ5|J-REGION| [len=51] (mis=5)
463-504 ==> 0-41 on |40|IGHG1|C-REGION| [len=884] (mis=1)
junction support: 1 umis using 19 reads
cdr3 = CARGSTSWYDYW at 396, score = 8 + 7
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 133
start codons at 54, 205, 252, 257, 289, 351
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 60.6 = GACGTGCGTTCGAATC-1

using 7646 reads

====================================================================================

graph has 4012 edges initially, 48 edges after simplification

total ucounts = 862
nonsolo ucounts = 347[2^160, 3^68, 4^39, 5^23, 6^11, 7^5, 8^6, 9, 10^4, 11, 13^2, 14^2, 16, 19, 46, 59, 152, 171, 186, 209, 213, 222, 232, 241^2, 267, 269, 272, 281, 291, 301, 307, 312, 358, 393, 488, 527]
surviving nonsolo ucounts = 23[46, 59, 152, 171, 186, 209, 213, 222, 232, 241^2, 267, 269, 272, 281, 291, 301, 307, 312, 358, 393, 488, 527]
ids = [3, 248, 629, 548, 625, 215, 403, 852, 83, 231, ...]

====================================================================================

UMI info for barcode GACGTGCGTTCGAATC-1 contig 1 = GGGGAGGAGT...
umi ACAGTATGCT = 230 reads: +388 validated
umi ACCGCCGGAA = 308 reads: -345X +3 -4XX +4 -1XX +6 -3XX +8 -1XX +13 invalidated
umi ACTGCCCGGG = 290 reads: +388 validated
umi ATCGTACACT = 242 reads: +388 validated
umi CCAGTTCATA = 240 reads: +388 validated
umi CCCCTTCACC = 282 reads: +388 validated
umi CTCGTACCAT = 269 reads: +388 validated
umi CTTAGTTGGG = 216 reads: +388 validated
umi GTGTTTAGCC = 298 reads: +388 validated
umi TACGTGGGTC = 328 reads: +160 -1XX +227 invalidated
umi TAGTGACCTT = 185 reads: +388 validated
umi TATCAAACCA = 146 reads: -361 +2 -3X +8 -1X +13 invalidated
umi TCCGGCGCCT = 262 reads: +388 validated
umi TCGTCGTCCG = 470 reads: -352X +4 -1X +6 -3X +8 -1XX +13 invalidated
umi TTCATTGGCC = 286 reads: +107 -2XX +1 -1XX +1 -4XX +1 -3XX +2 -3XX +263 invalidated
umi TTGAATGTCG = 319 reads: +388 validated
umi TTTTAGCCAG = 224 reads: +388 validated

UMI info for barcode GACGTGCGTTCGAATC-1 contig 2 = AGTGCTTTCT...
umi AAACATGGGC = 13 reads: -1X +11 -2X +56 -1XX +9 -1XX +4 -2XX +2 -1XX +3 -1XX +3 -1X +4 -15X +27 -1X +6 -1X +6 -1X +1 -1X +1 -1X +3 -3X +2 -72X +1 -2X +3 -1XX +1 -1XX +8 -1XX +5 -1XX +23 -1XX +14 -1X +5 -1X +3 -121 invalidated
umi GCGTTTCGTA = 339 reads: +436 validated

GOOD CONTIGS

TIG 1[bases=555]
0-31 ==> 0-31 on |226|IGKV1-33|5'UTR| [len=31] (mis=2)
31-382 ==> 0-351 on |227|IGKV1-33|L-REGION+V-REGION| [len=351] (mis=17)
381-419 ==> 0-38 on |215|IGKJ3|J-REGION| [len=38] (mis=4)
419-555 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 14 umis using 600 reads
cdr3 = CQQYDSLPVTF at 358, score = 9 + 8
umis assigned: [83, 98, 142, 231, 303, 311, 387, 403, 572, 603] and 7 others
of which 17 are surviving nonsolos
reads assigned: 4500
start codons at 31, 37, 93, 106, 245, 368, 461
confident = true

TIG 2[bases=487]
17-388 ==> 0-371 on |186|IGHV4-34|L-REGION+V-REGION| [len=371] (mis=0)
407-453 ==> 0-46 on |54|IGHJ4|J-REGION| [len=46] (mis=4)
453-487 ==> 0-34 on |6|IGHD|C-REGION| [len=1289] (mis=0)
junction support: 1 umis using 48 reads
cdr3 = CARGRRYFDRRLDYW at 377, score = 9 + 7
umis assigned: [3, 480]
of which 2 are surviving nonsolos
reads assigned: 345
start codons at 17, 38, 82, 168, 471
confident = true

REJECT CONTIGS

TIG 1[bases=489]
0-39 ==> 309-348 on rc of segment before IGHJ5 exon 1 [len=348] (mis=0)
89-246 ==> 0-157 on rc of segment before IGHJ3P exon 1 [len=157] (mis=0)
244-307 ==> 0-63 on |59|IGHJ6|J-REGION| [len=63] (mis=0)
307-489 ==> 0-182 on |40|IGHG1|C-REGION| [len=884] (mis=1)
umis assigned: [215, 248, 548, 744]
of which 4 are surviving nonsolos
reads assigned: 754
start codons at 96, 130, 169, 224, 264
confident = false
did not find CDR3
now this is a cell
paired!

GCCGCGGACACGGCTGTGTATTACTGTGCGAGAGGCCGGCGATATTTTGACCGACGACTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG <==> ATCAGCAGCCTGCAGCCTGAAGATATTGCAACATATTACTGTCAACAGTATGATAGTCTGCCGGTCACCTTCGGCCCTGGGACCAAAGTGGAGATCAAAC

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 60.7 = GACGTGCGTTCTCATT-1

using 267 reads

====================================================================================

graph has 118 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[6, 261]
surviving nonsolo ucounts = 1[261]
ids = [1]

====================================================================================

UMI info for barcode GACGTGCGTTCTCATT-1 contig 1 = GAGGAATCAG...
umi TCGCACACAG = 265 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=552]
28-379 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
378-416 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
416-552 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 44 reads
cdr3 = CLQYSSSPWTF at 355, score = 9 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 258
start codons at 28, 34, 103, 239, 458
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 60.9 = GACGTGCGTTTGGCGC-1

using 1036 reads

====================================================================================

graph has 372 edges initially, 6 edges after simplification

total ucounts = 7
nonsolo ucounts = 3[220, 264, 548]
surviving nonsolo ucounts = 3[220, 264, 548]
ids = [4, 3, 6]

====================================================================================

UMI info for barcode GACGTGCGTTTGGCGC-1 contig 1 = GGGAATCAGT...
umi GGTCTGTGCA = 58 reads: -345 +2 -1X +3 -3XX +2 -1XX +2 -1XX +3 -2XX +15 -1XX +7 invalidated
umi TCTTTTTCGT = 222 reads: +388 validated
umi TTCTCTGTGC = 554 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=551]
0-27 ==> 0-27 on |222|IGKV1-17|5'UTR| [len=27] (mis=1)
27-378 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
377-415 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
415-551 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 2 umis using 123 reads
cdr3 = CLQYSSSPWTF at 354, score = 9 + 8
umis assigned: [3, 4, 6]
of which 3 are surviving nonsolos
reads assigned: 816
start codons at 27, 33, 102, 238, 457
confident = true
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 60.11 = GACGTGCTCAAAGACA-1

using 72 reads

====================================================================================

graph has 87 edges initially, 2 edges after simplification

total ucounts = 18
nonsolo ucounts = 13[2^4, 4^3, 6^3, 8, 9, 12]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 60.14 = GACGTGCTCACAATGC-1

using 548 reads

====================================================================================

graph has 286 edges initially, 4 edges after simplification

total ucounts = 10
nonsolo ucounts = 3[2, 236, 303]
surviving nonsolo ucounts = 2[236, 303]
ids = [7, 5]

====================================================================================

UMI info for barcode GACGTGCTCACAATGC-1 contig 1 = GAATCAGTCC...
umi GGAATGCCTG = 280 reads: +388 validated

UMI info for barcode GACGTGCTCACAATGC-1 contig 2 = GAGAGGAGCC...
umi TTAACGGGGC = 216 reads: +442 validated

GOOD CONTIGS

TIG 1[bases=497]
0-25 ==> 2-27 on |222|IGKV1-17|5'UTR| [len=27] (mis=0)
25-376 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=0)
375-413 ==> 0-38 on |217|IGKJ5|J-REGION| [len=38] (mis=3)
413-497 ==> 0-84 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 53 reads
cdr3 = CLQHNSYPPTF at 352, score = 9 + 8
umis assigned: [5]
of which 1 are surviving nonsolos
reads assigned: 273
start codons at 25, 31, 100, 182, 236, 455
confident = false

TIG 2[bases=527]
0-71 ==> 8-79 on |119|IGHV3-21|5'UTR| [len=79] (mis=0)
71-424 ==> 0-353 on |120|IGHV3-21|L-REGION+V-REGION| [len=353] (mis=2)
422-445 ==> 0-23 on |21|IGHD3-3|D-REGION| [len=31] (mis=3)
450-513 ==> 0-63 on |737|IGHJ6|J-REGION| [len=63] (mis=2)
junction support: 1 umis using 20 reads
cdr3 = CAVDLRFLEWFLQDYYYYGMDVW at 413, score = 9 + 7
umis assigned: [7]
of which 1 are surviving nonsolos
reads assigned: 209
start codons at 71, 227, 374, 470
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 60.22 = GACGTGCTCCACTGGG-1

using 868 reads

====================================================================================

graph has 238 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 1[866]
surviving nonsolo ucounts = 1[866]
ids = [1]

====================================================================================

REJECT CONTIGS

TIG 1[bases=372]
0-50 ==> 9-59 on |62|IGHV1-18|5'UTR| [len=59] (mis=1)
0-50 ==> 11314-11364 on rc of segment before IGHV3-19 exon 1 [len=11364] (mis=1)
36-68 ==> 10108-10140 on rc of segment before IGHV1-18 exon 2 [len=10140] (mis=3)
50-193 ==> 0-143 on |63|IGHV1-18|L-REGION+V-REGION| [len=351] (mis=3)
218-372 ==> 6-160 on |1|IGHA1|C-REGION| [len=1058] (mis=0)
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 858
start codons at 50, 266
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 60.31 = GACGTGCTCCGATATG-1

using 8135 reads

====================================================================================

graph has 2270 edges initially, 46 edges after simplification

total ucounts = 124
nonsolo ucounts = 56[2^16, 3^8, 4^3, 5^5, 6, 7, 9^2, 10, 12, 60, 135, 144, 164, 188, 195, 236, 312, 367, 373, 382, 387, 441, 457, 658, 880, 919, 1623]
surviving nonsolo ucounts = 18[60, 135, 144, 164, 188, 195, 236, 312, 367, 373, 382, 387, 441, 457, 658, 880, 919, 1623]
ids = [65, 75, 24, 30, 33, 115, 104, 86, 17, 27, ...]

====================================================================================

UMI info for barcode GACGTGCTCCGATATG-1 contig 1 = GGGGGACTCC...
umi CACATATTGT = 376 reads: +290 -4XX +127 invalidated
umi CACCCGGTGT = 299 reads: +421 validated
umi CATCTGGGGC = 369 reads: +421 validated
umi CCCCTATGGC = 189 reads: +421 validated
umi CTACTTAGTC = 448 reads: +421 validated
umi GATCTAGGTC = 60 reads: +382 -10 +29 non-validated
umi TACACCCCAC = 315 reads: +421 validated
umi TTCCCTTCCT = 153 reads: +421 validated

UMI info for barcode GACGTGCTCCGATATG-1 contig 2 = GGGTGGCTGC...
umi GCGCAGTGCC = 663 reads: -206 +191 non-validated
umi TATTGGTCCC = 910 reads: -333X +1 -2XX +1 -3XX +1 -7XX +1 -6XX +1 -3XX +1 -1XX +1 -2XX +33 invalidated
umi TGCAACTTCC = 238 reads: +397 validated
umi TTCAGGTGTC = 244 reads: -361 +1 -7X +1 -2XX +2 -2XX +2 -2XX +1 -1XX +1 -1XX +1 -1XX +2 -5XX +1 -2XX +1 invalidated

GOOD CONTIGS

TIG 1[bases=513]
21-379 ==> 0-358 on |98|IGHV2-5|L-REGION+V-REGION| [len=358] (mis=8)
396-442 ==> 0-46 on |54|IGHJ4|J-REGION| [len=46] (mis=5)
442-513 ==> 0-71 on |60|IGHM|C-REGION| [len=1358] (mis=0)
junction support: 7 umis using 200 reads
cdr3 = CAHSAHNSAWRIDYW at 366, score = 7 + 7
umis assigned: [17, 19, 27, 33, 50, 65, 86, 115]
of which 8 are surviving nonsolos
reads assigned: 2163
start codons at 21, 65, 244, 247, 327, 336
confident = true

TIG 2[bases=646]
38-400 ==> 0-362 on |372|IGLV4-60|L-REGION+V-REGION| [len=362] (mis=4)
397-435 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=3)
435-646 ==> 0-211 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 2 umis using 185 reads
cdr3 = CETWASNTRMF at 374, score = 7 + 8
umis assigned: [73, 91, 104, 114]
of which 4 are surviving nonsolos
reads assigned: 2017
start codons at 38, 202, 242, 357, 401
confident = true

REJECT CONTIGS

TIG 1[bases=470]
0-347 ==> 6-353 on |253|IGKV1D-39|L-REGION+V-REGION| [len=353] (mis=15)
344-382 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=3)
382-470 ==> 0-88 on |212|IGKC|C-REGION| [len=320] (mis=0)
cdr3 = CQQSYSTPWQF at 321, score = 8 + 7
umis assigned: [30]
of which 1 are surviving nonsolos
reads assigned: 146
start codons at 0, 56, 69, 205, 424
confident = false
not full
VJ delta = 16
not full

TIG 2[bases=582]
0-49 ==> 0-49 on |208|IGHV7-4-1|5'UTR| [len=49] (mis=0)
0-49 ==> 5951-6000 on rc of segment after IGHV7-4-1 exon 1 [len=6000] (mis=0)
35-67 ==> 10108-10140 on rc of segment before IGHV1-18 exon 2 [len=10140] (mis=3)
49-402 ==> 0-353 on |209|IGHV7-4-1|L-REGION+V-REGION| [len=353] (mis=21)
434-480 ==> 0-46 on |54|IGHJ4|J-REGION| [len=46] (mis=6)
480-582 ==> 0-102 on |6|IGHD|C-REGION| [len=1289] (mis=0)
umis assigned: [24, 75]
of which 2 are surviving nonsolos
reads assigned: 260
start codons at 49, 205, 247, 252, 284, 346, 418, 498, 559
confident = false
frameshifted full length stopped transcript of length 582
did not find CDR3

TIG 3[bases=344]
5-87 ==> 1456-1538 on segment before IGLJ7 exon 1 [len=1538] (mis=0)
87-133 ==> 0-46 on |317|IGLJ7|J-REGION| [len=46] (mis=0)
133-344 ==> 0-211 on |308|IGLC7|C-REGION| [len=317] (mis=0)
umis assigned: [34, 43, 99]
of which 3 are surviving nonsolos
reads assigned: 2930
start codons at 265, 328
confident = false
did not find CDR3
now this is a cell
paired!

CCTGTGGACACAGGCACATATTACTGTGCACACAGTGCACATAACAGTGCCTGGAGAATTGATTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG <==> ATCTCCAACCTCCAGTTTGAGGATGAGGCTGATTATTACTGTGAGACCTGGGCCAGTAACACTCGAATGTTCGGCGGAGGGACCAAGCTGACCGTCCTAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 60.35 = GACGTGCTCGAACGGA-1

using 187 reads

====================================================================================

graph has 235 edges initially, 2 edges after simplification

total ucounts = 61
nonsolo ucounts = 39[2^11, 3^9, 4^7, 5^3, 6^3, 7, 8^2, 9, 10, 13]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 60.37 = GACGTGCTCGATCCCT-1

using 259 reads

====================================================================================

graph has 118 edges initially, 2 edges after simplification

total ucounts = 16
nonsolo ucounts = 10[2^3, 3^3, 5, 6, 13, 214]
surviving nonsolo ucounts = 1[214]
ids = [2]

====================================================================================

UMI info for barcode GACGTGCTCGATCCCT-1 contig 1 = AGAGCTGCTC...
umi ATGTTGCACA = 196 reads: +391 validated

GOOD CONTIGS

TIG 1[bases=507]
0-31 ==> 21-52 on |282|IGKV3-20|5'UTR| [len=52] (mis=0)
31-379 ==> 0-348 on |283|IGKV3-20|L-REGION+V-REGION| [len=348] (mis=9)
384-422 ==> 0-38 on |215|IGKJ3|J-REGION| [len=38] (mis=3)
422-507 ==> 0-85 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 37 reads
cdr3 = CQQYDSSPPGVTF at 355, score = 9 + 8
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 195
start codons at 31, 239, 338, 365, 464
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 60.38 = GACGTGCTCGCGATCG-1

using 262 reads

====================================================================================

graph has 108 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[2^2, 257]
surviving nonsolo ucounts = 1[257]
ids = [1]

====================================================================================

UMI info for barcode GACGTGCTCGCGATCG-1 contig 1 = CTGGGCCTCA...
umi ACAGTCAGAC = 242 reads: +379 validated

GOOD CONTIGS

TIG 1[bases=546]
0-37 ==> 15-52 on |348|IGLV3-1|5'UTR| [len=52] (mis=0)
37-371 ==> 0-334 on |349|IGLV3-1|L-REGION+V-REGION| [len=346] (mis=11)
378-416 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=1)
416-546 ==> 0-130 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 44 reads
cdr3 = CQAWDSTTDWVF at 352, score = 6 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 235
start codons at 37, 42, 98, 185, 331, 335
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 60.40 = GACGTGCTCGCTTGTC-1

using 132 reads

====================================================================================

graph has 58 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 1[130]
surviving nonsolo ucounts = 1[130]
ids = [2]

====================================================================================

UMI info for barcode GACGTGCTCGCTTGTC-1 contig 1 = GAGGAACTGC...
umi GTTGTATCTT = 116 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=471]
0-33 ==> 14-47 on |278|IGKV3-11|5'UTR| [len=47] (mis=0)
33-378 ==> 0-345 on |279|IGKV3-11|L-REGION+V-REGION| [len=345] (mis=5)
377-415 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=5)
415-471 ==> 0-56 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 24 reads
cdr3 = CQQRSNWPPTF at 354, score = 9 + 8
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 116
start codons at 33, 238, 241, 457
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 60.47 = GACGTGCTCTCCCTGA-1

using 218 reads

====================================================================================

graph has 80 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[2^2, 212]
surviving nonsolo ucounts = 1[212]
ids = [0]

====================================================================================

UMI info for barcode GACGTGCTCTCCCTGA-1 contig 1 = GGGGTCACAA...
umi ATCTCCCCAT = 201 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=514]
0-38 ==> 124-162 on |342|IGLV2-23|5'UTR| [len=162] (mis=0)
38-378 ==> 0-340 on |343|IGLV2-23|L-REGION+V-REGION| [len=340] (mis=5)
394-432 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=1)
432-514 ==> 0-82 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 33 reads
cdr3 = CCSYAGSRTPNWVF at 362, score = 8 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 197
start codons at 38, 177, 239, 246, 372
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 60.64 = GACGTTAAGCTACCTA-1

using 429 reads

====================================================================================

graph has 170 edges initially, 4 edges after simplification

total ucounts = 10
nonsolo ucounts = 5[2^2, 4, 179, 237]
surviving nonsolo ucounts = 2[179, 237]
ids = [8, 5]

====================================================================================

UMI info for barcode GACGTTAAGCTACCTA-1 contig 1 = TGAGCGCAGA...
umi TTTCTCTGAG = 176 reads: +391 validated

GOOD CONTIGS

TIG 1[bases=525]
0-36 ==> 0-36 on |330|IGLV1-51|5'UTR| [len=36] (mis=0)
36-389 ==> 0-353 on |331|IGLV1-51|L-REGION+V-REGION| [len=353] (mis=6)
389-427 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=0)
427-525 ==> 0-98 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 23 reads
cdr3 = CGTWDSSLSVPVVF at 357, score = 7 + 8
umis assigned: [8]
of which 1 are surviving nonsolos
reads assigned: 175
start codons at 36, 190, 241, 365
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 60.68 = GACGTTAAGGTAGCCA-1

using 11101 reads

====================================================================================

graph has 4698 edges initially, 58 edges after simplification

total ucounts = 687
nonsolo ucounts = 323[2^127, 3^80, 4^23, 5^24, 6^12, 7^8, 8^5, 9^3, 10^2, 11^4, 12^2, 13, 22, 35, 41, 60, 96, 122^2, 141, 142, 161, 165, 179, 184, 199, 202, 207, 210, 219, 228, 234, 237^2, 246, 266, 273, 292, 393, 394, 719, 826, 1055, 1827]
surviving nonsolo ucounts = 29[60, 96, 122^2, 141, 142, 161, 165, 179, 184, 199, 202, 207, 210, 219, 228, 234, 237^2, 246, 266, 273, 292, 393, 394, 719, 826, 1055, 1827]
ids = [82, 15, 405, 480, 554, 521, 504, 615, 78, 272, ...]

====================================================================================

UMI info for barcode GACGTTAAGGTAGCCA-1 contig 1 = TCTGAGGATA...
umi AACAGTGATA = 95 reads: +391 validated
umi AGTCCTGCAA = 226 reads: +391 validated
umi ATTACTGATC = 293 reads: +391 validated
umi CAGTTCTTGG = 235 reads: +391 validated
umi CATCCATGTA = 1719 reads: -327X +1 -1XX +1 -2XX +2 -1XX +1 -1XX +1 -3XX +1 -7XX +1 -3XX +1 -2XX +3 -1XX +31 invalidated
umi CCTTTTTCGT = 211 reads: +391 validated
umi CTTGCGGTAA = 219 reads: +391 validated
umi GAATGCTGTC = 122 reads: +391 validated
umi GGACAGCCCG = 190 reads: -327X +1 -1XX +1 -2XX +2 -1XX +1 -1XX +1 -3XX +1 -7XX +1 -3XX +1 -2XX +3 -1XX +31 invalidated
umi GGGATTGAAG = 828 reads: -327 +2 -3XX +2 -1XX +1 -1XX +1 -3XX +1 -7XX +1 -3XX +1 -2XX +3 -1XX +31 invalidated
umi GGGCACCACA = 1051 reads: -344 +47 non-validated
umi GGTTGTTCCT = 122 reads: +391 validated
umi GTTGGTAGGG = 141 reads: +391 validated
umi TATTGCCCTT = 139 reads: +386 -2X +3 invalidated
umi TGCGGAGCAA = 719 reads: -353X +1 -2XX +3 -1XX +31 invalidated
umi TGGATCATAT = 171 reads: +391 validated
umi TGTCTACCCA = 246 reads: +391 validated
umi TTACTAATAC = 200 reads: +391 validated
umi TTTTATTCTC = 233 reads: +391 validated

UMI info for barcode GACGTTAAGGTAGCCA-1 contig 2 = AGCTCTGGGA...
umi AACATGCTGT = 403 reads: -104 +1 -2XX +1 -4XX +1 -11XX +312 invalidated
umi AAGCAGCGTG = 204 reads: +436 validated
umi AATACAATAC = 274 reads: +436 validated
umi ACGTATAACA = 177 reads: +436 validated
umi ACGTTTCTTT = 60 reads: +363 -73 non-validated
umi ACTCAGAACG = 259 reads: +436 validated
umi CCGCTGACTC = 186 reads: +434 -2 non-validated
umi CGCTATGTGA = 238 reads: +431 -5 non-validated
umi GTCTATATGG = 150 reads: +436 validated
umi TCCGTTTGTC = 202 reads: +339 -4 +93 non-validated

GOOD CONTIGS

TIG 1[bases=688]
0-86 ==> 0-86 on |732|IGLV6-57|5'UTR| [len=86] (mis=0)
86-439 ==> 0-353 on |384|IGLV6-57|L-REGION+V-REGION| [len=353] (mis=2)
439-477 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=0)
477-688 ==> 0-211 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 14 umis using 387 reads
cdr3 = CQSYDSSNHVVF at 413, score = 6 + 8
umis assigned: [15, 133, 174, 217, 226, 297, 389, 405, 464, 474] and 9 others
of which 19 are surviving nonsolos
reads assigned: 7048
start codons at 86, 149, 240, 291, 423, 438
confident = true

TIG 2[bases=587]
0-80 ==> 0-80 on |139|IGHV3-43|5'UTR| [len=80] (mis=3)
80-435 ==> 0-355 on |140|IGHV3-43|L-REGION+V-REGION| [len=355] (mis=28)
468-516 ==> 0-48 on |55|IGHJ4|J-REGION| [len=48] (mis=2)
516-587 ==> 0-71 on |60|IGHM|C-REGION| [len=1358] (mis=0)
junction support: 6 umis using 124 reads
cdr3 = CAKHGGYDWAYDGESRYFDYW at 422, score = 9 + 7
umis assigned: [16, 31, 35, 78, 82, 88, 272, 313, 504, 565]
of which 10 are surviving nonsolos
reads assigned: 2120
start codons at 80, 225, 231, 236, 315, 383, 432
confident = true
now this is a cell
paired!

TATTACTGTGCAAAACATGGTGGCTACGATTGGGCATACGACGGAGAATCACGGTACTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG <==> TCTGGACTGAAGACTGAGGACGAGGCTGACTACTACTGTCAGTCTTATGATAGCAGCAATCATGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 60.74 = GACGTTAAGTCAATAG-1

using 41466 reads

====================================================================================

graph has 13921 edges initially, 204 edges after simplification

total ucounts = 1696
nonsolo ucounts = 880[2^269, 3^180, 4^107, 5^79, 6^43, 7^31, 8^13, 9^10, 10^8, 11^2, 12^4, 13^2, 14, 15, 16, 18, 19, 24, 25, 26, 28, 31, 33, 37^2, 38, 41, 44, 46, 47, 58, 61, 65^2, 67, 68, 74, 83^2, 90, 94, 95, 97, 100, 104, 108, 110^2, 113, 115, 119, 122, 125, 147^2, 148, 155^2, 163, 169, 176, 179, 181, 183, 184, 186, 191, 195, 197, 201, 203^2, 204, 207, 208, 212, 214, 216, 218, 225, 229, 239, 241^2, 250, 255, 256, 257, 258, 260, 263^2, 264, 265, 266, 268, 271, 274^2, 275, 276, 277, 278^2, 283, 285, 288^2, 294^2, 301, 304^2, 310, 311, 313, 324, 328, 339, 353, 376, 403, 436, 497, 502, 504, 541, 585, 586, 599, 641, 674, 701, 759, 855, 865, 882, 886, 980, 1100, 1155, 1157, 1195, 2095]
surviving nonsolo ucounts = 110[2^2, 44, 47, 58, 61, 65, 67, 68, 74, 83^2, 90, 94, 97, 100, 108, 110, 115, 122, 125, 147, 148, 155^2, 163, 169, 176, 179, 183, 184, 186, 191, 195, 197, 201, 203^2, 204, 207, 208, 212, 214, 216, 218, 225, 229, 239, 241^2, 250, 255, 256, 257, 258, 260, 263^2, 264, 265, 266, 268, 271, 274^2, 275, 276, 277, 278^2, 283, 285, 288^2, 294^2, 301, 304^2, 310, 311, 313, 324, 328, 339, 353, 376, 403, 436, 497, 502, 504, 541, 585, 586, 599, 641, 674, 701, 759, 855, 865, 882, 886, 980, 1100, 1155, 1157, 1195, 2095]
ids = [183, 1440, 642, 980, 616, 562, 288, 911, 768, 236, ...]

====================================================================================

UMI info for barcode GACGTTAAGTCAATAG-1 contig 1 = AGTGCTTTCT...
umi AGATATACAG = 99 reads: +442 validated
umi AGCTTCTCTC = 85 reads: +339 -4XX +2 -4X +93 invalidated
umi AGGCAGGTTC = 197 reads: +442 validated
umi ATCATGTGCT = 78 reads: +442 validated
umi ATGCTGCGTG = 166 reads: +442 validated
umi ATGTTGGTGC = 65 reads: +442 validated
umi CAGTAATCTT = 65 reads: +40 -1 +1 -3XX +2 -3XX +3 -2XX +1 -6XX +2 -1XX +1 -1XX +364 -11 invalidated
umi CCATATCTCT = 198 reads: +442 validated
umi CCTGTATACC = 60 reads: +442 validated
umi CGCTATAATA = 60 reads: -12 +430 non-validated
umi CGTAAGGTTT = 43 reads: +314 -1 +4 -1 +80 -12 +30 non-validated
umi CTATATCGAT = 197 reads: +442 validated
umi CTCTATCGGA = 185 reads: +442 validated
umi CTTATGGATA = 67 reads: +442 validated
umi GAGGCCTGCA = 120 reads: +442 validated
umi GATGTCTACT = 268 reads: +442 validated
umi GCCTAGCTGA = 66 reads: +339 -4X +2 -4X +93 invalidated
umi GCTTCTGCTA = 94 reads: +442 validated
umi GGATCTAGAT = 45 reads: +262 -1 +179 non-validated
umi GGGATACCGA = 153 reads: +442 validated
umi GTAACCCCCT = 207 reads: +442 validated
umi TACATTTCTG = 181 reads: +442 validated
umi TAGTACCGTA = 114 reads: +442 validated
umi TCCGGCATGA = 4 reads: -393 +3 -1XX +1 -1XX +2 -1XX +2 -4XX +13 -1XX +20 invalidated
umi TGCCCACCCG = 87 reads: +442 validated
umi TTGCCATGCT = 154 reads: +442 validated

GOOD CONTIGS

TIG 1[bases=641]
17-388 ==> 0-371 on |186|IGHV4-34|L-REGION+V-REGION| [len=371] (mis=29)
411-459 ==> 0-48 on |55|IGHJ4|J-REGION| [len=48] (mis=6)
459-641 ==> 0-182 on |40|IGHG1|C-REGION| [len=884] (mis=1)
junction support: 22 umis using 364 reads
cdr3 = CARAPKDRNHYNGFDYW at 377, score = 7 + 6
umis assigned: [145, 175, 181, 236, 279, 288, 403, 481, 562, 616] and 16 others
of which 25 are surviving nonsolos
reads assigned: 2970
start codons at 17, 38, 82, 171, 368
confident = true

REJECT CONTIGS

TIG 1[bases=604]
4-88 ==> 5927-6011 on segment before IGLV2-5 exon 1 [len=6137] (mis=0)
42-67 ==> 0-25 on |337|IGLV2-11|L-REGION+V-REGION| [len=358] (mis=0)
67-329 ==> 26-288 on |337|IGLV2-11|L-REGION+V-REGION| [len=358] (mis=34) [SHIFT!]
355-393 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=8)
393-604 ==> 0-211 on |306|IGLC2|C-REGION| [len=317] (mis=0)
umis assigned: [13, 24, 30, 38, 62, 86, 120, 133, 142, 160] and 30 others
of which 40 are surviving nonsolos
reads assigned: 13189
start codons at 42, 180, 192, 242, 252, 299, 320
confident = false
did not find CDR3

TIG 2[bases=340]
3-45 ==> 3108-3150 on segment before IGLJ5 exon 1 [len=3161] (mis=4)
91-129 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=0)
129-340 ==> 0-211 on |306|IGLC2|C-REGION| [len=317] (mis=0)
umis assigned: [68, 118, 281, 282, 432, 499, 873, 1038, 1069, 1079] and 3 others
of which 13 are surviving nonsolos
reads assigned: 10302
start codons at 72
confident = false
did not find CDR3

TIG 3[bases=636]
0-43 ==> 0-43 on |362|IGLV3-25|5'UTR| [len=43] (mis=0)
1-53 ==> 2818-2870 on segment before IGLV3-13 exon 1 [len=3075] (mis=4)
15-90 ==> 11269-11344 on segment before IGLV3-6 exon 1 [len=11502] (mis=3)
43-380 ==> 0-337 on |363|IGLV3-25|L-REGION+V-REGION| [len=337] (mis=12)
387-425 ==> 0-38 on |309|IGLJ1|J-REGION| [len=38] (mis=1)
425-636 ==> 0-211 on |305|IGLC1|C-REGION| [len=317] (mis=0)
cdr3 = CQSADSSGSYYVF at 358, score = 8 + 8
umis assigned: [0, 66, 74, 85, 87, 280, 324, 532, 760, 761] and 15 others
of which 25 are surviving nonsolos
reads assigned: 7932
start codons at 43, 104, 173, 191, 235, 389, 557
confident = false
not full
full length stopped transcript of length 636
frameshifted full length stopped transcript of length 636
VJ delta = 12
not full

TIG 4[bases=664]
0-63 ==> 0-63 on |297|IGKV5-2|5'UTR| [len=63] (mis=0)
63-112 ==> 0-49 on |298|IGKV5-2|L-REGION+V-REGION| [len=345] (mis=0)
112-407 ==> 50-345 on |298|IGKV5-2|L-REGION+V-REGION| [len=345] (mis=29) [SHIFT!]
409-447 ==> 0-38 on |217|IGKJ5|J-REGION| [len=38] (mis=3)
447-526 ==> 0-79 on rc of segment before IGKJ5 exon 1 [len=2884] (mis=8)
528-664 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
cdr3 = CLQHDDFALITF at 383, score = 6 + 8
umis assigned: [190, 392, 472, 483, 1600]
of which 5 are surviving nonsolos
reads assigned: 1454
start codons at 63, 148, 152, 210, 218, 223, 353, 366, 393, 396, 488, 570
confident = false
not full
frameshifted full length stopped transcript of length 664
VJ delta = 15
not full
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 60.83 = GACGTTACAACTGGCC-1

using 384 reads

====================================================================================

graph has 150 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 2[2, 378]
surviving nonsolo ucounts = 1[378]
ids = [2]

====================================================================================

UMI info for barcode GACGTTACAACTGGCC-1 contig 1 = GGAGTCAGAC...
umi GTTGCAATAA = 383 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=550]
0-26 ==> 155-181 on |232|IGKV1-5|5'UTR| [len=181] (mis=0)
26-377 ==> 0-351 on |233|IGKV1-5|L-REGION+V-REGION| [len=351] (mis=2)
376-414 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
414-550 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 68 reads
cdr3 = CQQYNSYFWTF at 353, score = 8 + 8
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 375
start codons at 26, 32, 88, 101, 333, 456
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 60.92 = GACGTTACACACCGCA-1

using 434 reads

====================================================================================

graph has 174 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[433]
surviving nonsolo ucounts = 1[433]
ids = [1]

====================================================================================

UMI info for barcode GACGTTACACACCGCA-1 contig 1 = ATACTTTCTG...
umi ATTCTCTTGG = 388 reads: +433 validated

GOOD CONTIGS

TIG 1[bases=530]
0-37 ==> 27-64 on |191|IGHV4-4|5'UTR| [len=64] (mis=2)
37-390 ==> 0-353 on |192|IGHV4-4|L-REGION+V-REGION| [len=353] (mis=3)
432-470 ==> 8-46 on |54|IGHJ4|J-REGION| [len=46] (mis=0)
470-530 ==> 0-60 on |6|IGHD|C-REGION| [len=1289] (mis=0)
junction support: 1 umis using 37 reads
cdr3 = CASPPGYYGDYDGRGGLGYW at 379, score = 9 + 7
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 379
start codons at 16, 37, 81, 488
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 60.93 = GACGTTACACATCCAA-1

using 32 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[2, 30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 60.95 = GACGTTACACATTCGA-1

using 86 reads

====================================================================================

graph has 56 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[2^2, 3, 77]
surviving nonsolo ucounts = 1[77]
ids = [2]

====================================================================================

REJECT CONTIGS

TIG 1[bases=391]
0-276 ==> 75-351 on |233|IGKV1-5|L-REGION+V-REGION| [len=351] (mis=2)
276-313 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=3)
313-391 ==> 0-78 on |212|IGKC|C-REGION| [len=320] (mis=0)
cdr3 = CQQYNSYPRTF at 252, score = 8 + 8
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 52
start codons at 0, 232, 355
confident = false
not full
VJ delta = 13
not full
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 60.98 = GACGTTACACGGTGTC-1

using 596 reads

====================================================================================

graph has 160 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 2[3, 586]
surviving nonsolo ucounts = 1[586]
ids = [6]

====================================================================================

REJECT CONTIGS

TIG 1[bases=373]
1-46 ==> 8883-8928 on segment before IGKV1OR2-3 exon 1 [len=9099] (mis=3)
42-202 ==> 193-353 on |253|IGKV1D-39|L-REGION+V-REGION| [len=353] (mis=5)
200-237 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=0)
237-373 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
cdr3 = CQQSYTTLLTF at 176, score = 9 + 9
umis assigned: [6]
of which 1 are surviving nonsolos
reads assigned: 582
start codons at 28, 34, 60, 279
confident = false
not full
VJ delta = 15
not full
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 60.102 = GACGTTACAGACAAAT-1

using 65 reads

====================================================================================

graph has 56 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 5[4^2, 5, 7, 41]
surviving nonsolo ucounts = 1[41]
ids = [2]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 60.103 = GACGTTACAGCCTATA-1

using 321 reads

====================================================================================

graph has 144 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 1[319]
surviving nonsolo ucounts = 1[319]
ids = [0]

====================================================================================

UMI info for barcode GACGTTACAGCCTATA-1 contig 1 = GGGGATCAGT...
umi AAGTTCCCGT = 317 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=551]
0-27 ==> 0-27 on |222|IGKV1-17|5'UTR| [len=27] (mis=2)
27-378 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=1)
377-415 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=2)
415-551 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 43 reads
cdr3 = CLQHNSYPLTF at 354, score = 9 + 9
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 312
start codons at 27, 33, 102, 184, 238, 457
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 60.104 = GACGTTACAGCGATCC-1

using 64 reads

====================================================================================

graph has 94 edges initially, 2 edges after simplification

total ucounts = 13
nonsolo ucounts = 10[2^3, 3, 4, 5, 6, 7, 8, 22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 60.110 = GACGTTACAGTGGAGT-1

using 415 reads

====================================================================================

graph has 120 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 1[411]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================

REJECT CONTIGS

TIG 1[bases=392]
0-218 ==> 62-280 on rc of segment before IGKJ4 exon 1 [len=280] (mis=12)
218-256 ==> 0-38 on |217|IGKJ5|J-REGION| [len=38] (mis=1)
256-392 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
umis assigned: [4]
of which 0 are surviving nonsolos
reads assigned: 406
start codons at 54, 298
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 60.117 = GACGTTACATGCCACG-1

using 1039 reads

====================================================================================

graph has 368 edges initially, 14 edges after simplification

total ucounts = 7
nonsolo ucounts = 3[2, 285, 748]
surviving nonsolo ucounts = 2[285, 748]
ids = [6, 1]

====================================================================================

UMI info for barcode GACGTTACATGCCACG-1 contig 1 = GGGAGTCAGT...
umi TTTTATCTGA = 284 reads: +385 validated

UMI info for barcode GACGTTACATGCCACG-1 contig 2 = GGGAGTCAGT...
umi GACATCACTT = 756 reads: -235 +153 non-validated

GOOD CONTIGS

TIG 1[bases=552]
0-31 ==> 0-31 on |226|IGKV1-33|5'UTR| [len=31] (mis=5)
31-382 ==> 0-351 on |227|IGKV1-33|L-REGION+V-REGION| [len=351] (mis=2)
379-416 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=0)
416-552 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 47 reads
cdr3 = CQQYDNLLTF at 358, score = 9 + 9
umis assigned: [6]
of which 1 are surviving nonsolos
reads assigned: 280
start codons at 31, 37, 93, 106, 245, 368, 458
confident = false

TIG 2[bases=555]
31-382 ==> 0-351 on |231|IGKV1-39|L-REGION+V-REGION| [len=351] (mis=21)
381-419 ==> 0-38 on |217|IGKJ5|J-REGION| [len=38] (mis=0)
419-555 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 142 reads
cdr3 = CQQSYRRPITF at 358, score = 8 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 740
start codons at 31, 37, 93, 106, 245, 341, 461
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 60.124 = GACGTTAGTACCGTTA-1

using 1501 reads

====================================================================================

graph has 1251 edges initially, 36 edges after simplification

total ucounts = 247
nonsolo ucounts = 101[2^44, 3^20, 4^13, 5^9, 6^4, 7^2, 8^2, 10, 14, 100, 173, 208, 271, 280]
surviving nonsolo ucounts = 6[5, 100, 173, 208, 271, 280]
ids = [107, 50, 202, 9, 168, 224]

====================================================================================

UMI info for barcode GACGTTAGTACCGTTA-1 contig 1 = ATCAGTCCCA...
umi GCGTTACTCA = 129 reads: +48 -1XX +13 -1XX +26 -1XX +3 -1XX +10 -1XX +5 -1XX +5 -1XX +7 -27X +3 -2XX +1 -5XX +2 -1XX +1 -1XX +5 -1XX +8 -1XX +20 -1XX +3 -1XX +2 -2XX +3 -1XX +3 -1XX +4 -1XX +6 -1XX +4 -2XX +21 -2XX +14 -2XX +11 -1XX +6 -32 +1 -3XX +1 -3XX +1 -1XX +3 -5XX +3 -1XX +3 -1XX +7 -1XX +20 -1XX +4 -1XX +2 invalidated
umi TGGCTCTGGG = 267 reads: +22 -1XX +365 invalidated

UMI info for barcode GACGTTAGTACCGTTA-1 contig 2 = GGAGCCCCAG...
umi AACCGCGGAC = 212 reads: +427 validated
umi ATACCTTACC = 101 reads: +427 validated
umi CCTTGTCTGC = 5 reads: -85 +2 -1 +1 -4 +1 -2 +5 -1 +1 -3 +69 -39 +57 -156 non-validated
umi TCCACAACCG = 170 reads: +420 -7 non-validated

GOOD CONTIGS

TIG 1[bases=490]
0-23 ==> 4-27 on |222|IGKV1-17|5'UTR| [len=27] (mis=0)
23-374 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=18)
373-411 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
411-490 ==> 0-79 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 47 reads
cdr3 = CLQYSSSPWTF at 350, score = 9 + 8
umis assigned: [168, 224]
of which 2 are surviving nonsolos
reads assigned: 388
start codons at 23, 29, 98, 234, 453
confident = true

TIG 2[bases=572]
0-68 ==> 168-236 on |170|IGHV3-74|5'UTR| [len=236] (mis=0)
68-421 ==> 0-353 on |171|IGHV3-74|L-REGION+V-REGION| [len=353] (mis=19)
451-495 ==> 19-63 on |737|IGHJ6|J-REGION| [len=63] (mis=0)
495-572 ==> 0-77 on |45|IGHG3|C-REGION| [len=1130] (mis=0)
junction support: 2 umis using 24 reads
cdr3 = CARDRISITISFPSMDVW at 410, score = 9 + 7
umis assigned: [9, 50, 107, 202]
of which 4 are surviving nonsolos
reads assigned: 469
start codons at 68, 224, 260, 285, 371, 452
confident = true
now this is a cell
paired!

ACGGCTCTGTATTACTGTGCAAGAGATCGGATAAGTATTACGATATCCTTCCCCTCTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCAG <==> ATCAGCAGCCTGCAGCCTGAAGATTTTGCGACTTATTACTGTCTACAGTATTCTAGTTCCCCGTGGACGTTTGGCCAAGGGACCAAGGTGGAAATCAAAC

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 60.125 = GACGTTAGTAGGGTAC-1

using 392 reads

====================================================================================

graph has 82 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 3[2^2, 384]
surviving nonsolo ucounts = 1[384]
ids = [5]

====================================================================================

REJECT CONTIGS

TIG 1[bases=497]
0-321 ==> 30-351 on |339|IGLV2-14|L-REGION+V-REGION| [len=361] (mis=0)
320-358 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=0)
358-497 ==> 0-139 on |306|IGLC2|C-REGION| [len=317] (mis=0)
cdr3 = CSSYTSSSTVVF at 294, score = 8 + 8
umis assigned: [5]
of which 1 are surviving nonsolos
reads assigned: 335
start codons at 127, 171, 178
confident = false
not full
VJ delta = 27
not full
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 60.127 = GACGTTAGTATATGAG-1

using 565 reads

====================================================================================

graph has 188 edges initially, 4 edges after simplification

total ucounts = 7
nonsolo ucounts = 3[2, 35, 524]
surviving nonsolo ucounts = 2[35, 524]
ids = [6, 0]

====================================================================================

UMI info for barcode GACGTTAGTATATGAG-1 contig 1 = GCTCTGCTTC...
umi AACATCAGGA = 529 reads: +391 validated

GOOD CONTIGS

TIG 1[bases=653]
0-51 ==> 0-51 on |320|IGLV1-40|5'UTR| [len=51] (mis=0)
51-407 ==> 0-356 on |321|IGLV1-40|L-REGION+V-REGION| [len=356] (mis=12)
423-442 ==> 19-38 on |309|IGLJ1|J-REGION| [len=38] (mis=1)
442-653 ==> 0-211 on |305|IGLC1|C-REGION| [len=317] (mis=1)
junction support: 1 umis using 72 reads
cdr3 = CQSYDSSLSGYVF at 375, score = 8 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 518
start codons at 51, 205, 259, 358, 385, 406, 574
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 60.128 = GACGTTAGTATTCGTG-1

using 445 reads

====================================================================================

graph has 150 edges initially, 4 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[83, 361]
surviving nonsolo ucounts = 2[83, 361]
ids = [0, 2]

====================================================================================

UMI info for barcode GACGTTAGTATTCGTG-1 contig 1 = TGGGGAGGAA...
umi TCCAGTCAGC = 336 reads: +379 validated

GOOD CONTIGS

TIG 1[bases=490]
0-37 ==> 10-47 on |278|IGKV3-11|5'UTR| [len=47] (mis=0)
37-382 ==> 0-345 on |279|IGKV3-11|L-REGION+V-REGION| [len=345] (mis=5)
379-416 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=0)
416-490 ==> 0-74 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 60 reads
cdr3 = CQQRSTPLTF at 358, score = 9 + 9
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 333
start codons at 37, 242, 245, 458
confident = false

REJECT CONTIGS

TIG 1[bases=440]
0-336 ==> 20-356 on |321|IGLV1-40|L-REGION+V-REGION| [len=356] (mis=22)
333-371 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=6)
371-440 ==> 0-69 on |307|IGLC3|C-REGION| [len=317] (mis=0)
cdr3 = CQSYDSILTGWAF at 304, score = 7 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 70
start codons at 134, 287, 314
confident = false
not full
VJ delta = 22
not full
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 60.134 = GACGTTAGTCGGATCC-1

using 28 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[2, 25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 60.139 = GACGTTAGTGATAAGT-1

using 353 reads

====================================================================================

graph has 186 edges initially, 4 edges after simplification

total ucounts = 15
nonsolo ucounts = 10[2, 3^3, 6, 7^2, 8, 11, 298]
surviving nonsolo ucounts = 1[298]
ids = [14]

====================================================================================

UMI info for barcode GACGTTAGTGATAAGT-1 contig 1 = GAGAACCTGC...
umi TTTTCAACCG = 286 reads: +379 validated

GOOD CONTIGS

TIG 1[bases=589]
0-52 ==> 0-52 on |348|IGLV3-1|5'UTR| [len=52] (mis=1)
52-390 ==> 0-338 on |349|IGLV3-1|L-REGION+V-REGION| [len=346] (mis=0)
393-431 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=0)
431-589 ==> 0-158 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 35 reads
cdr3 = CQAWDSSTPWVF at 367, score = 6 + 8
umis assigned: [14]
of which 1 are surviving nonsolos
reads assigned: 278
start codons at 52, 57, 113, 200, 346, 350
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 60.140 = GACGTTAGTGCCTTGG-1

using 98 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[2, 94]
surviving nonsolo ucounts = 1[94]
ids = [1]

====================================================================================

UMI info for barcode GACGTTAGTGCCTTGG-1 contig 1 = CCCTCCTTGG...
umi ATTGAAAGGA = 91 reads: +436 validated

GOOD CONTIGS

TIG 1[bases=502]
0-39 ==> 20-59 on |64|IGHV1-18|5'UTR| [len=59] (mis=1)
39-392 ==> 0-353 on |65|IGHV1-18|L-REGION+V-REGION| [len=353] (mis=22)
441-475 ==> 12-46 on |54|IGHJ4|J-REGION| [len=46] (mis=0)
475-502 ==> 0-27 on |1|IGHA1|C-REGION| [len=1058] (mis=0)
junction support: 1 umis using 8 reads
cdr3 = CARDKHFRDPYYHYSGALDHW at 381, score = 7 + 7
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 90
start codons at 39, 237, 242, 259, 303, 336
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 60.142 = GACGTTAGTGTATGGG-1

using 505 reads

====================================================================================

graph has 192 edges initially, 4 edges after simplification

total ucounts = 7
nonsolo ucounts = 3[2, 213, 286]
surviving nonsolo ucounts = 2[213, 286]
ids = [3, 1]

====================================================================================

UMI info for barcode GACGTTAGTGTATGGG-1 contig 1 = GGAGTCAGTC...
umi CATCAATTAG = 289 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=550]
0-26 ==> 21-47 on |230|IGKV1-39|5'UTR| [len=47] (mis=0)
26-377 ==> 0-351 on |231|IGKV1-39|L-REGION+V-REGION| [len=351] (mis=11)
376-414 ==> 0-38 on |217|IGKJ5|J-REGION| [len=38] (mis=0)
414-550 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 42 reads
cdr3 = CQQSYRRPITF at 353, score = 8 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 283
start codons at 26, 32, 88, 101, 237, 336, 456
confident = false

REJECT CONTIGS

TIG 1[bases=552]
0-68 ==> 5924-5992 on segment before IGLV2-5 exon 1 [len=6137] (mis=4)
41-168 ==> 0-127 on |339|IGLV2-14|L-REGION+V-REGION| [len=361] (mis=3)
168-366 ==> 136-334 on |339|IGLV2-14|L-REGION+V-REGION| [len=361] (mis=29)
386-417 ==> 7-38 on |311|IGLJ2|J-REGION| [len=38] (mis=2)
417-552 ==> 0-135 on |306|IGLC2|C-REGION| [len=317] (mis=0)
cdr3 = CSSSKGSATLF at 356, score = 6 + 8
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 207
start codons at 41, 240
confident = false
see deletion of 9 bases at pos 127 on |339|IGLV2-14|L-REGION+V-REGION|
not full
full length transcript of length 552
VJ delta = 36
delta too large
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 60.144 = GACGTTAGTTCGTGAT-1

using 388 reads

====================================================================================

graph has 108 edges initially, 4 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[172, 214]
surviving nonsolo ucounts = 2[172, 214]
ids = [3, 2]

====================================================================================

UMI info for barcode GACGTTAGTTCGTGAT-1 contig 1 = GGACTCCTGT...
umi CCCAAGCGGC = 167 reads: +433 validated

UMI info for barcode GACGTTAGTTCGTGAT-1 contig 2 = GCTCTGCTTC...
umi CATTAGGATA = 211 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=474]
18-376 ==> 0-358 on |98|IGHV2-5|L-REGION+V-REGION| [len=358] (mis=19)
401-451 ==> 0-50 on |53|IGHJ3|J-REGION| [len=50] (mis=4)
451-474 ==> 0-23 on |40|IGHG1|C-REGION| [len=884] (mis=0)
junction support: 1 umis using 17 reads
cdr3 = CAHQLAIVTGYYENAFDIW at 363, score = 8 + 8
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 166
start codons at 18, 62, 241, 244, 247, 333, 403
confident = false

TIG 2[bases=524]
0-51 ==> 0-51 on |320|IGLV1-40|5'UTR| [len=51] (mis=0)
51-407 ==> 0-356 on |321|IGLV1-40|L-REGION+V-REGION| [len=356] (mis=12)
407-445 ==> 0-38 on |309|IGLJ1|J-REGION| [len=38] (mis=1)
445-524 ==> 0-79 on |305|IGLC1|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 36 reads
cdr3 = CQSYDSSLSGLYVF at 375, score = 7 + 8
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 207
start codons at 51, 205, 208, 259, 358, 385, 409
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 60.153 = GACGTTATCACCGGGT-1

using 27 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 60.155 = GACGTTATCAGCTTAG-1

using 545 reads

====================================================================================

graph has 224 edges initially, 30 edges after simplification

total ucounts = 6
nonsolo ucounts = 5[3^2, 4, 202, 332]
surviving nonsolo ucounts = 2[202, 332]
ids = [5, 1]

====================================================================================

UMI info for barcode GACGTTATCAGCTTAG-1 contig 1 = AGGAGTCAGT...
umi CACCACAGTT = 332 reads: +385 validated

UMI info for barcode GACGTTATCAGCTTAG-1 contig 2 = GTCAGTCTCA...
umi TTGATAACTC = 204 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=548]
27-380 ==> 0-353 on |253|IGKV1D-39|L-REGION+V-REGION| [len=353] (mis=18)
373-412 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=5)
412-548 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 53 reads
cdr3 = CQQSYSTPSF at 354, score = 9 + 7
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 328
start codons at 27, 33, 89, 102, 238, 454
confident = false

TIG 2[bases=547]
0-23 ==> 0-23 on |252|IGKV1D-39|5'UTR| [len=23] (mis=0)
23-376 ==> 0-353 on |253|IGKV1D-39|L-REGION+V-REGION| [len=353] (mis=13)
374-411 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=0)
411-547 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 34 reads
cdr3 = CQQSYTTLLTF at 350, score = 9 + 9
umis assigned: [5]
of which 1 are surviving nonsolos
reads assigned: 200
start codons at 23, 29, 85, 98, 234, 453
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 60.157 = GACGTTATCAGTTAGC-1

using 229 reads

====================================================================================

graph has 60 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 2[2, 223]
surviving nonsolo ucounts = 1[223]
ids = [4]

====================================================================================

UMI info for barcode GACGTTATCAGTTAGC-1 contig 1 = GATCAGGACT...
umi TTACAACATG = 223 reads: +397 validated

GOOD CONTIGS

TIG 1[bases=563]
0-30 ==> 0-30 on |264|IGKV2-28|5'UTR| [len=30] (mis=0)
30-390 ==> 0-360 on |265|IGKV2-28|L-REGION+V-REGION| [len=360] (mis=0)
389-427 ==> 0-38 on |215|IGKJ3|J-REGION| [len=38] (mis=4)
427-563 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 28 reads
cdr3 = CMQALQTPPTF at 366, score = 9 + 8
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 221
start codons at 30, 63, 99, 187, 349, 369, 469
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 60.160 = GACGTTATCCAAATGC-1

using 195 reads

====================================================================================

graph has 64 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 1[193]
surviving nonsolo ucounts = 1[193]
ids = [1]

====================================================================================

UMI info for barcode GACGTTATCCAAATGC-1 contig 1 = GTCAGACCCT...
umi TAAGCCCCTT = 196 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=547]
0-29 ==> 24-53 on |236|IGKV1-8|5'UTR| [len=53] (mis=1)
29-374 ==> 0-345 on |237|IGKV1-8|L-REGION+V-REGION| [len=345] (mis=22)
383-411 ==> 11-39 on |214|IGKJ2|J-REGION| [len=39] (mis=1)
411-547 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 34 reads
cdr3 = CQQYYSYPRTF at 350, score = 9 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 193
start codons at 23, 29, 85, 98, 234, 453
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 60.165 = GACGTTATCGGAAATA-1

using 46 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[46]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 60.170 = GACGTTATCTCATTCA-1

using 308 reads

====================================================================================

graph has 122 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 3[2^2, 298]
surviving nonsolo ucounts = 1[298]
ids = [2]

====================================================================================

UMI info for barcode GACGTTATCTCATTCA-1 contig 1 = ACCCAAAAAC...
umi ATCATCGACC = 285 reads: +436 validated

GOOD CONTIGS

TIG 1[bases=504]
0-54 ==> 5-59 on |64|IGHV1-18|5'UTR| [len=59] (mis=1)
54-407 ==> 0-353 on |65|IGHV1-18|L-REGION+V-REGION| [len=353] (mis=22)
456-490 ==> 12-46 on |54|IGHJ4|J-REGION| [len=46] (mis=0)
junction support: 1 umis using 27 reads
cdr3 = CARDKHFRDPYYHYSGALDHW at 396, score = 7 + 7
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 283
start codons at 54, 252, 257, 274, 318, 351
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 60.180 = GACTAACAGAAGGACA-1

using 375 reads

====================================================================================

graph has 98 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 3[2^2, 367]
surviving nonsolo ucounts = 1[367]
ids = [1]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 60.185 = GACTAACAGAGCTGCA-1

using 221 reads

====================================================================================

graph has 82 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 2[2, 216]
surviving nonsolo ucounts = 1[216]
ids = [3]

====================================================================================

UMI info for barcode GACTAACAGAGCTGCA-1 contig 1 = GTGGGCTCAG...
umi TCACCCTGAT = 211 reads: +385 validated

GOOD CONTIGS

TIG 1[bases=544]
0-35 ==> 0-35 on |350|IGLV3-10|5'UTR| [len=35] (mis=0)
35-382 ==> 0-347 on |351|IGLV3-10|L-REGION+V-REGION| [len=347] (mis=4)
382-420 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=3)
420-544 ==> 0-124 on |307|IGLC3|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 29 reads
cdr3 = CYSTDSSGNPSQVF at 350, score = 7 + 8
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 210
start codons at 35, 96, 165, 183, 234, 296, 333
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 60.186 = GACTAACAGATAGTCA-1

using 23 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 60.192 = GACTAACAGCACCGCT-1

using 890 reads

====================================================================================

graph has 388 edges initially, 4 edges after simplification

total ucounts = 9
nonsolo ucounts = 4[2, 224, 308, 351]
surviving nonsolo ucounts = 3[224, 308, 351]
ids = [1, 2, 7]

====================================================================================

UMI info for barcode GACTAACAGCACCGCT-1 contig 1 = AGGAGTCAGT...
umi AGCATGTGCT = 320 reads: +188 -1XX +196 invalidated
umi TGTGCTACAA = 352 reads: +385 validated

GOOD CONTIGS

TIG 1[bases=548]
0-27 ==> 4-31 on |226|IGKV1-33|5'UTR| [len=31] (mis=0)
27-378 ==> 0-351 on |227|IGKV1-33|L-REGION+V-REGION| [len=351] (mis=2)
374-412 ==> 0-38 on |217|IGKJ5|J-REGION| [len=38] (mis=3)
412-548 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 2 umis using 123 reads
cdr3 = CQQYDNLPTF at 354, score = 9 + 8
umis assigned: [2, 7]
of which 2 are surviving nonsolos
reads assigned: 645
start codons at 27, 33, 89, 102, 241, 364, 454
confident = true
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 60.200 = GACTAACAGCTAGCCC-1

using 429 reads

====================================================================================

graph has 134 edges initially, 4 edges after simplification

total ucounts = 6
nonsolo ucounts = 3[4, 207, 215]
surviving nonsolo ucounts = 2[207, 215]
ids = [5, 0]

====================================================================================

UMI info for barcode GACTAACAGCTAGCCC-1 contig 1 = AGCTTCAGCT...
umi GTTTTTAGCA = 204 reads: +388 validated

UMI info for barcode GACTAACAGCTAGCCC-1 contig 2 = TGGGGGTGAC...
umi AACAAAACTC = 210 reads: +433 validated

GOOD CONTIGS

TIG 1[bases=496]
0-47 ==> 67-114 on |322|IGLV1-44|5'UTR| [len=114] (mis=0)
47-400 ==> 0-353 on |323|IGLV1-44|L-REGION+V-REGION| [len=353] (mis=4)
397-435 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=2)
435-496 ==> 0-61 on |307|IGLC3|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 31 reads
cdr3 = CAAWDDSLNGPVF at 368, score = 8 + 8
umis assigned: [5]
of which 1 are surviving nonsolos
reads assigned: 203
start codons at 47, 351, 376, 381, 393
confident = false

TIG 2[bases=485]
24-382 ==> 0-358 on |98|IGHV2-5|L-REGION+V-REGION| [len=358] (mis=19)
407-457 ==> 0-50 on |53|IGHJ3|J-REGION| [len=50] (mis=4)
457-485 ==> 0-28 on |40|IGHG1|C-REGION| [len=884] (mis=0)
junction support: 1 umis using 23 reads
cdr3 = CAHQLAIVTGYYENAFDIW at 369, score = 8 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 204
start codons at 24, 68, 247, 250, 253, 339, 409
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 60.204 = GACTAACAGGACAGCT-1

using 5935 reads

====================================================================================

graph has 4385 edges initially, 26 edges after simplification

total ucounts = 791
nonsolo ucounts = 371[2^138, 3^90, 4^40, 5^29, 6^12, 7^15, 8^7, 9^4, 10^4, 11^4, 12^5, 13, 14, 19, 20, 36, 52, 102, 106, 123, 164, 174, 205, 217, 221, 222, 233, 294, 298, 313, 326, 332, 368, 399]
surviving nonsolo ucounts = 16[19, 52, 102, 164, 174, 205, 217, 221, 222, 294, 298, 313, 326, 332, 368, 399]
ids = [619, 569, 522, 579, 768, 362, 614, 527, 440, 40, ...]

====================================================================================

UMI info for barcode GACTAACAGGACAGCT-1 contig 1 = AGCTCTGGGA...
umi AACGCATGTA = 337 reads: +433 validated
umi CGAACACCTT = 400 reads: +433 validated
umi CTACGCCCCC = 191 reads: +410 -23 non-validated
umi GTGATCCGCG = 102 reads: +422 -11 non-validated
umi GTTTATCTTT = 227 reads: +433 validated
umi TACTTATAGA = 51 reads: +433 validated
umi TAGGCATCTA = 140 reads: +433 validated
umi TCACCTCCTT = 215 reads: +433 validated
umi TCACGTATTC = 285 reads: +433 validated
umi TTTGCCTTTG = 307 reads: +424 -9 non-validated

UMI info for barcode GACTAACAGGACAGCT-1 contig 2 = AGGAGTCAGA...
umi AATATTTTCG = 297 reads: +388 validated
umi AGGCATGATA = 312 reads: +388 validated
umi TAGTGGATCT = 324 reads: +388 validated
umi TCAGATTTCG = 20 reads: +18 -29 +173 -1 +60 -1X +8 -5 +93 invalidated

GOOD CONTIGS

TIG 1[bases=612]
0-77 ==> 0-77 on |149|IGHV3-64|5'UTR| [len=77] (mis=0)
77-433 ==> 0-356 on |150|IGHV3-64|L-REGION+V-REGION| [len=356] (mis=4)
460-510 ==> 0-50 on |53|IGHJ3|J-REGION| [len=50] (mis=0)
510-612 ==> 0-102 on |6|IGHD|C-REGION| [len=1289] (mis=0)
junction support: 7 umis using 107 reads
cdr3 = CARGFSEGSGWYGDAFDIW at 422, score = 8 + 8
umis assigned: [19, 321, 362, 522, 527, 569, 579, 614, 616, 777]
of which 10 are surviving nonsolos
reads assigned: 2215
start codons at 77, 231, 236, 276, 297, 315, 383, 407, 456, 462, 491, 528, 589
confident = true

TIG 2[bases=551]
0-27 ==> 154-181 on |232|IGKV1-5|5'UTR| [len=181] (mis=1)
27-378 ==> 0-351 on |233|IGKV1-5|L-REGION+V-REGION| [len=351] (mis=7)
377-415 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=0)
415-551 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 3 umis using 132 reads
cdr3 = CQQYNSYPWTF at 354, score = 8 + 8
umis assigned: [40, 131, 581, 619]
of which 4 are surviving nonsolos
reads assigned: 941
start codons at 27, 33, 89, 102, 238, 241, 334, 457
confident = true
now this is a cell
paired!

GCTGTGTATTACTGTGCGAGAGGATTTTCGGAGGGCAGTGGCTGGTATGGTGATGCTTTTGATATCTGGGGCCAAGGGACAATGGTCACCGTCTCTTCAG <==> ATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACAGTATAATAGTTATCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAAC

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 60.207 = GACTAACAGGCAGGTT-1

using 359 reads

====================================================================================

graph has 158 edges initially, 2 edges after simplification

total ucounts = 12
nonsolo ucounts = 8[2, 3^2, 4, 7, 8, 9, 319]
surviving nonsolo ucounts = 1[319]
ids = [9]

====================================================================================

UMI info for barcode GACTAACAGGCAGGTT-1 contig 1 = GCTGTGCTGT...
umi GTTTTTCGCG = 313 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=543]
0-43 ==> 0-43 on |362|IGLV3-25|5'UTR| [len=43] (mis=0)
43-380 ==> 0-337 on |363|IGLV3-25|L-REGION+V-REGION| [len=337] (mis=0)
387-425 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=1)
425-543 ==> 0-118 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 56 reads
cdr3 = CQSADSSGTYVVF at 358, score = 8 + 7
umis assigned: [9]
of which 1 are surviving nonsolos
reads assigned: 308
start codons at 43, 104, 173, 191, 386, 402
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 60.216 = GACTAACAGTAGCGGT-1

using 622 reads

====================================================================================

graph has 232 edges initially, 12 edges after simplification

total ucounts = 9
nonsolo ucounts = 4[2, 4, 152, 459]
surviving nonsolo ucounts = 2[152, 459]
ids = [3, 8]

====================================================================================

UMI info for barcode GACTAACAGTAGCGGT-1 contig 1 = TGGGGAGGAG...
umi CTAGCTGAGT = 157 reads: +385 validated

GOOD CONTIGS

TIG 1[bases=553]
32-385 ==> 0-353 on |253|IGKV1D-39|L-REGION+V-REGION| [len=353] (mis=18)
378-417 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=5)
417-553 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 26 reads
cdr3 = CQQSYSTPSF at 359, score = 9 + 7
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 151
start codons at 32, 38, 94, 107, 243, 459
confident = false

REJECT CONTIGS

TIG 1[bases=567]
5-81 ==> 5532-5608 on segment before IGKV2OR2-1 exon 1 [len=6000] (mis=2)
36-396 ==> 0-360 on |265|IGKV2-28|L-REGION+V-REGION| [len=360] (mis=3)
393-431 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=3)
431-567 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
umis assigned: [8]
of which 1 are surviving nonsolos
reads assigned: 452
start codons at 36, 69, 105, 193, 355, 375, 473
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 60.217 = GACTAACAGTCGCCGT-1

using 685 reads

====================================================================================

graph has 790 edges initially, 6 edges after simplification

total ucounts = 170
nonsolo ucounts = 82[2^28, 3^21, 4^13, 5^3, 6^3, 7^3, 9^4, 10, 11, 13, 14^2, 15, 259]
surviving nonsolo ucounts = 1[259]
ids = [132]

====================================================================================

UMI info for barcode GACTAACAGTCGCCGT-1 contig 1 = GGGGTCACAA...
umi TCCGTTTCTT = 258 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=543]
0-38 ==> 124-162 on |342|IGLV2-23|5'UTR| [len=162] (mis=0)
38-378 ==> 0-340 on |343|IGLV2-23|L-REGION+V-REGION| [len=340] (mis=5)
394-432 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=1)
432-543 ==> 0-111 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 35 reads
cdr3 = CCSYAGSRTPNWVF at 362, score = 8 + 8
umis assigned: [132]
of which 1 are surviving nonsolos
reads assigned: 253
start codons at 38, 177, 239, 246, 372
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 60.222 = GACTAACAGTGGTAAT-1

using 28 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[28]
surviving nonsolo ucounts = 1[28]
ids = [0]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 60.223 = GACTAACAGTGTCTCA-1

using 232 reads

====================================================================================

graph has 98 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 3[3, 8, 215]
surviving nonsolo ucounts = 1[215]
ids = [8]

====================================================================================

UMI info for barcode GACTAACAGTGTCTCA-1 contig 1 = GAGGAATCAG...
umi TCTCTTTCGT = 216 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=552]
28-379 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
378-416 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
416-552 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 39 reads
cdr3 = CLQYSSSPWTF at 355, score = 9 + 8
umis assigned: [8]
of which 1 are surviving nonsolos
reads assigned: 211
start codons at 28, 34, 103, 239, 458
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 60.225 = GACTAACCAAACCCAT-1

using 304 reads

====================================================================================

graph has 126 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 2[4, 297]
surviving nonsolo ucounts = 1[297]
ids = [1]

====================================================================================

UMI info for barcode GACTAACCAAACCCAT-1 contig 1 = ATCACACAAC...
umi GCCTTAGGTT = 293 reads: +418 validated

GOOD CONTIGS

TIG 1[bases=493]
0-57 ==> 3-60 on |68|IGHV1-24|5'UTR| [len=60] (mis=0)
57-408 ==> 0-351 on |69|IGHV1-24|L-REGION+V-REGION| [len=351] (mis=44)
427-475 ==> 0-48 on |55|IGHJ4|J-REGION| [len=48] (mis=6)
junction support: 1 umis using 37 reads
cdr3 = CATAAGINKWAFDSW at 399, score = 10 + 7
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 288
start codons at 57, 202, 213, 255, 277, 321, 354, 425
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 60.227 = GACTAACCAAAGCAAT-1

using 489 reads

====================================================================================

graph has 212 edges initially, 4 edges after simplification

total ucounts = 6
nonsolo ucounts = 2[214, 271]
surviving nonsolo ucounts = 2[214, 271]
ids = [5, 1]

====================================================================================

UMI info for barcode GACTAACCAAAGCAAT-1 contig 1 = GGGGTCACAA...
umi ACTCCTCCGG = 267 reads: +394 validated

UMI info for barcode GACTAACCAAAGCAAT-1 contig 2 = TCAGTCAGGA...
umi TCTTTTCCAT = 200 reads: +391 validated

GOOD CONTIGS

TIG 1[bases=514]
0-38 ==> 124-162 on |342|IGLV2-23|5'UTR| [len=162] (mis=0)
38-378 ==> 0-340 on |343|IGLV2-23|L-REGION+V-REGION| [len=340] (mis=0)
394-432 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=0)
432-514 ==> 0-82 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 36 reads
cdr3 = CCSYAGSSTFYVVF at 362, score = 8 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 264
start codons at 38, 177, 239, 246, 372, 393
confident = false

TIG 2[bases=501]
16-367 ==> 0-351 on |233|IGKV1-5|L-REGION+V-REGION| [len=351] (mis=9)
368-407 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=4)
407-501 ==> 0-94 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 27 reads
cdr3 = CQQYNNYSPYTF at 343, score = 8 + 8
umis assigned: [5]
of which 1 are surviving nonsolos
reads assigned: 198
start codons at 16, 22, 78, 91, 227, 230, 323, 449
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 60.230 = GACTAACCAAGCCGCT-1

using 6139 reads

====================================================================================

graph has 2487 edges initially, 46 edges after simplification

total ucounts = 310
nonsolo ucounts = 137[2^52, 3^27, 4^16, 5^10, 6^3, 8, 9, 10, 12^4, 14, 17, 154, 187, 205, 212, 215, 221, 226, 229, 253, 272, 288, 309, 310, 312, 322, 345, 349, 367, 369, 398]
surviving nonsolo ucounts = 20[154, 187, 205, 212, 215, 221, 226, 229, 253, 272, 288, 309, 310, 312, 322, 345, 349, 367, 369, 398]
ids = [28, 79, 239, 111, 229, 4, 68, 212, 90, 301, ...]

====================================================================================

UMI info for barcode GACTAACCAAGCCGCT-1 contig 1 = GATTCCTCCT...
umi AAATGATGTG = 221 reads: +424 validated
umi AGAGGCACAG = 322 reads: +424 validated
umi TCCACCTAAG = 204 reads: +424 validated

UMI info for barcode GACTAACCAAGCCGCT-1 contig 2 = GGATTTTATA...
umi ACTGAACCCC = 290 reads: +400 validated
umi CATCTTCCTT = 256 reads: +400 validated
umi CGATTATCCC = 215 reads: +400 validated
umi GTTAGGTAAT = 231 reads: +400 validated

GOOD CONTIGS

TIG 1[bases=569]
0-43 ==> 15-58 on |89|IGHV1-69D|5'UTR| [len=58] (mis=0)
43-396 ==> 0-353 on |88|IGHV1-69D|L-REGION+V-REGION| [len=353] (mis=0)
405-436 ==> 0-31 on |20|IGHD3-22|D-REGION| [len=31] (mis=6)
433-467 ==> 12-46 on |54|IGHJ4|J-REGION| [len=46] (mis=0)
467-569 ==> 0-102 on |6|IGHD|C-REGION| [len=1289] (mis=0)
junction support: 3 umis using 62 reads
cdr3 = CARDQSPHYYDSSGYSW at 385, score = 9 + 7
umis assigned: [4, 41, 239]
of which 3 are surviving nonsolos
reads assigned: 737
start codons at 43, 194, 241, 340, 413, 485, 546
confident = true

TIG 2[bases=600]
0-64 ==> 111-175 on |295|IGKV4-1|5'UTR| [len=175] (mis=0)
64-427 ==> 0-363 on |296|IGKV4-1|L-REGION+V-REGION| [len=363] (mis=1)
425-464 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=4)
464-600 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 4 umis using 147 reads
cdr3 = CQQYYSTPYTF at 403, score = 9 + 8
umis assigned: [37, 90, 111, 212]
of which 4 are surviving nonsolos
reads assigned: 974
start codons at 64, 133, 386, 506
confident = true

REJECT CONTIGS

TIG 1[bases=552]
3-84 ==> 5665-5746 on segment before IGKV1OR2-6 exon 1 [len=5879] (mis=3)
27-378 ==> 0-351 on |231|IGKV1-39|L-REGION+V-REGION| [len=351] (mis=4)
378-416 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=2)
416-552 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
umis assigned: [6, 28, 68, 79, 105, 123, 126, 185, 229, 243] and 3 others
of which 13 are surviving nonsolos
reads assigned: 3754
start codons at 27, 33, 89, 102, 238, 458
confident = false
did not find CDR3
now this is a cell
paired!

GACACGGCCGTGTATTACTGTGCGAGAGACCAGAGCCCACATTACTATGATAGTAGTGGTTATTCTTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG <==> ATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATAGTACCCCTTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAAC

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 60.235 = GACTAACCAATTGCTG-1

using 240 reads

====================================================================================

graph has 150 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 3[2, 6, 228]
surviving nonsolo ucounts = 1[228]
ids = [3]

====================================================================================

UMI info for barcode GACTAACCAATTGCTG-1 contig 1 = GGGGTCTCAG...
umi GAACACGACG = 232 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=531]
0-38 ==> 4-42 on |336|IGLV2-11|5'UTR| [len=42] (mis=0)
38-382 ==> 0-344 on |337|IGLV2-11|L-REGION+V-REGION| [len=358] (mis=20)
388-426 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=3)
426-531 ==> 0-105 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 34 reads
cdr3 = CCSHAGSYIWVF at 362, score = 8 + 7
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 225
start codons at 38, 174, 177, 195, 249, 345, 372
confident = false
NOT paired!
sorting bam, mem = 0.09
running samtools sort -l 8G -o /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk060-ucc6a7a72ce/files/contig_bam_sorted.bam /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk060-ucc6a7a72ce/files/contig_bam.bam

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

20.918 seconds used processing barcodes, peak mem = 0.25
