[stdout]

starting vdj_asm_asm, mem = 0.01
available mem = 844.21 GB
/scratch/etanis/software/cellranger-6.1.2/lib/bin/cr_vdj martian assembly main /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk052-ucc6a7a72ce /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk052-ucc6a7a72ce/files /scratch/etanis/ST-118/multi/dimitri-multi/journal/SC_MULTI_CS.SC_MULTI_CORE.MULTI_GEM_WELL_PROCESSOR.VDJ_B_GEM_WELL_PROCESSOR.SC_VDJ_CONTIG_ASSEMBLER.ASSEMBLE_VDJ.fork0.chnk052.ucc6a7a72ce
n50_n50_rpu = 288

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.0 = CTGCTGTCACTCAGGC-1

using 26 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 2[2, 18]
surviving nonsolo ucounts = 1[18]
ids = [2]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.8 = CTGCTGTCAGCAGTTT-1

using 268 reads

====================================================================================

graph has 108 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[267]
surviving nonsolo ucounts = 1[267]
ids = [0]

====================================================================================

UMI info for barcode CTGCTGTCAGCAGTTT-1 contig 1 = AGGAATCAGT...
umi TACCAGGGGA = 243 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=505]
0-27 ==> 0-27 on |222|IGKV1-17|5'UTR| [len=27] (mis=0)
27-378 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
377-415 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
415-505 ==> 0-90 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 44 reads
cdr3 = CLQYSSSPWTF at 354, score = 9 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 238
start codons at 27, 33, 102, 238, 457
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.11 = CTGCTGTCAGCTTAAC-1

using 347 reads

====================================================================================

graph has 92 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 1[342]
surviving nonsolo ucounts = 1[342]
ids = [5]

====================================================================================

UMI info for barcode CTGCTGTCAGCTTAAC-1 contig 1 = GGGAAATACT...
umi GTTTATATTC = 257 reads: +406 validated

GOOD CONTIGS

TIG 1[bases=467]
0-42 ==> 103-145 on |193|IGHV4-59|5'UTR| [len=145] (mis=0)
42-392 ==> 0-350 on |194|IGHV4-59|L-REGION+V-REGION| [len=350] (mis=0)
400-448 ==> 0-48 on |55|IGHJ4|J-REGION| [len=48] (mis=2)
448-467 ==> 0-19 on |60|IGHM|C-REGION| [len=1358] (mis=0)
junction support: 1 umis using 33 reads
cdr3 = CARDGDYPFDYW at 381, score = 9 + 7
umis assigned: [5]
of which 1 are surviving nonsolos
reads assigned: 255
start codons at 42, 86
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.13 = CTGCTGTCAGGCTGAA-1

using 102 reads

====================================================================================

graph has 160 edges initially, 2 edges after simplification

total ucounts = 31
nonsolo ucounts = 22[2^6, 3^5, 4, 5^5, 6^2, 7, 9^2]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.22 = CTGCTGTCATACTACG-1

using 609 reads

====================================================================================

graph has 190 edges initially, 4 edges after simplification

total ucounts = 6
nonsolo ucounts = 2[268, 337]
surviving nonsolo ucounts = 2[268, 337]
ids = [3, 5]

====================================================================================

UMI info for barcode CTGCTGTCATACTACG-1 contig 1 = TCTGCTTCAG...
umi GGAATTATGC = 260 reads: +394 validated

UMI info for barcode CTGCTGTCATACTACG-1 contig 2 = AGTCCCAACC...
umi TGCGGGCGTG = 338 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=593]
0-49 ==> 2-51 on |320|IGLV1-40|5'UTR| [len=51] (mis=0)
49-388 ==> 0-339 on |321|IGLV1-40|L-REGION+V-REGION| [len=356] (mis=12)
405-443 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=3)
443-593 ==> 0-150 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 44 reads
cdr3 = CQSYDGGQSASAVF at 373, score = 7 + 8
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 258
start codons at 49, 203, 206, 257, 356, 383
confident = false

TIG 2[bases=544]
0-20 ==> 11-31 on |226|IGKV1-33|5'UTR| [len=31] (mis=0)
20-371 ==> 0-351 on |227|IGKV1-33|L-REGION+V-REGION| [len=351] (mis=29)
369-408 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=5)
408-544 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 52 reads
cdr3 = CHQYESVPYTF at 347, score = 9 + 7
umis assigned: [5]
of which 1 are surviving nonsolos
reads assigned: 337
start codons at 20, 26, 82, 95, 234, 330, 357, 450
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.25 = CTGCTGTCATAGGATA-1

using 71 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 2[4, 62]
surviving nonsolo ucounts = 1[62]
ids = [0]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.30 = CTGCTGTCATCCTAGA-1

using 56 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[56]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.33 = CTGCTGTCATGAAGTA-1

using 263 reads

====================================================================================

graph has 88 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 3[2^2, 256]
surviving nonsolo ucounts = 1[256]
ids = [3]

====================================================================================

REJECT CONTIGS

TIG 1[bases=482]
0-27 ==> 0-27 on |220|IGKV1-16|5'UTR| [len=27] (mis=0)
0-33 ==> 5967-6000 on rc of segment after IGKV1OR2-118 exon 1 [len=6000] (mis=2)
10-74 ==> 5672-5736 on segment before IGKV1OR2-6 exon 1 [len=5879] (mis=2)
12-84 ==> 8895-8967 on segment before IGKV1OR2-3 exon 1 [len=9099] (mis=8)
12-84 ==> 8904-8976 on rc of segment before IGKV2OR2-10 exon 1 [len=9109] (mis=8)
12-84 ==> 8903-8975 on segment before IGKV1OR2-11 exon 1 [len=9108] (mis=8)
27-349 ==> 0-322 on |221|IGKV1-16|L-REGION+V-REGION| [len=351] (mis=0)
352-390 ==> 0-38 on |215|IGKJ3|J-REGION| [len=38] (mis=0)
390-482 ==> 0-92 on |212|IGKC|C-REGION| [len=320] (mis=0)
cdr3 = CNFLFTF at 341, score = 3 + 8
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 226
start codons at 27, 33, 89, 102, 238, 432
confident = false
not full
frameshifted full length transcript of length 482
VJ delta = 27
not full
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.46 = CTGCTGTGTAGCGCAA-1

using 1671 reads

====================================================================================

graph has 2546 edges initially, 34 edges after simplification

total ucounts = 951
nonsolo ucounts = 372[2^210, 3^83, 4^35, 5^23, 6^10, 7^3, 8^3, 9, 10, 12, 15, 17]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.50 = CTGCTGTGTCAAAGAT-1

using 251 reads

====================================================================================

graph has 72 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 1[249]
surviving nonsolo ucounts = 1[249]
ids = [2]

====================================================================================

UMI info for barcode CTGCTGTGTCAAAGAT-1 contig 1 = GAATCAGTCC...
umi TTTTTCCCCC = 252 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=549]
0-25 ==> 2-27 on |222|IGKV1-17|5'UTR| [len=27] (mis=0)
25-376 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=6)
375-413 ==> 0-38 on |215|IGKJ3|J-REGION| [len=38] (mis=0)
413-549 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 36 reads
cdr3 = CLQHKSYPFTF at 352, score = 9 + 8
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 248
start codons at 25, 31, 100, 236, 455
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.75 = CTGCTGTGTGATGCCC-1

using 105 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[105]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.76 = CTGCTGTGTGATGTCT-1

using 356 reads

====================================================================================

graph has 76 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 2[3, 348]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================

UMI info for barcode CTGCTGTGTGATGTCT-1 contig 1 = GGGATACTTT...
umi GTTGTACTGC = 307 reads: +439 validated

GOOD CONTIGS

TIG 1[bases=507]
40-388 ==> 0-348 on |740|IGHV4-30-4|L-REGION+V-REGION| [len=348] (mis=5)
396-427 ==> 0-31 on |21|IGHD3-3|D-REGION| [len=31] (mis=1)
431-479 ==> 0-48 on |55|IGHJ4|J-REGION| [len=48] (mis=1)
479-507 ==> 0-28 on |60|IGHM|C-REGION| [len=1358] (mis=0)
junction support: 1 umis using 31 reads
cdr3 = CARAYYDFWSGYYNWVYFDYW at 385, score = 9 + 7
umis assigned: [4]
of which 0 are surviving nonsolos
reads assigned: 305
start codons at 40, 84
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.82 = CTGCTGTGTGTTCGAT-1

using 316 reads

====================================================================================

graph has 72 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 2[2, 310]
surviving nonsolo ucounts = 1[310]
ids = [4]

====================================================================================

UMI info for barcode CTGCTGTGTGTTCGAT-1 contig 1 = GGGGAGGAAC...
umi TAGATTTCTA = 310 reads: +379 validated

GOOD CONTIGS

TIG 1[bases=551]
0-36 ==> 61-97 on |280|IGKV3-15|5'UTR| [len=97] (mis=0)
36-381 ==> 0-345 on |281|IGKV3-15|L-REGION+V-REGION| [len=345] (mis=2)
377-415 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
415-551 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 54 reads
cdr3 = CQQYNNWRTF at 357, score = 9 + 8
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 307
start codons at 36, 105, 241, 457
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.103 = CTGCTGTTCAAGCCTA-1

using 249 reads

====================================================================================

graph has 136 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[249]
surviving nonsolo ucounts = 1[249]
ids = [0]

====================================================================================

UMI info for barcode CTGCTGTTCAAGCCTA-1 contig 1 = GGGGTCACAA...
umi CGCCATTCAC = 242 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=591]
0-38 ==> 124-162 on |342|IGLV2-23|5'UTR| [len=162] (mis=0)
38-378 ==> 0-340 on |343|IGLV2-23|L-REGION+V-REGION| [len=340] (mis=29)
394-432 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=6)
432-591 ==> 0-159 on |306|IGLC2|C-REGION| [len=317] (mis=3)
junction support: 1 umis using 35 reads
cdr3 = CCSEAGGGVPGLLF at 362, score = 7 + 9
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 238
start codons at 38, 192
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.105 = CTGCTGTTCACATGCA-1

using 179 reads

====================================================================================

graph has 279 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[179]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.120 = CTGCTGTTCCAAATGC-1

using 1019 reads

====================================================================================

graph has 1386 edges initially, 30 edges after simplification

total ucounts = 518
nonsolo ucounts = 211[2^91, 3^52, 4^25, 5^21, 6^9, 7^5, 8^3, 9^2, 11, 12, 15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.135 = CTGCTGTTCCTCTAGC-1

using 314 reads

====================================================================================

graph has 130 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[2, 3, 307]
surviving nonsolo ucounts = 1[307]
ids = [2]

====================================================================================

UMI info for barcode CTGCTGTTCCTCTAGC-1 contig 1 = GAGCTGCTCA...
umi CTAGAATCTA = 311 reads: +385 validated

GOOD CONTIGS

TIG 1[bases=551]
0-30 ==> 22-52 on |282|IGKV3-20|5'UTR| [len=52] (mis=0)
30-378 ==> 0-348 on |283|IGKV3-20|L-REGION+V-REGION| [len=348] (mis=4)
376-415 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=5)
415-551 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 44 reads
cdr3 = CQQYGSSPVTF at 354, score = 9 + 8
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 303
start codons at 30, 238, 241, 364, 457
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.138 = CTGCTGTTCGATAGAA-1

using 5152 reads

====================================================================================

graph has 5280 edges initially, 70 edges after simplification

total ucounts = 1475
nonsolo ucounts = 741[2^270, 3^170, 4^105, 5^67, 6^33, 7^35, 8^20, 9^11, 10^9, 11^2, 13^3, 14^4, 32, 41, 69, 89, 97, 114, 127, 196, 204, 218, 257, 260]
surviving nonsolo ucounts = 12[14, 41, 69, 89, 97, 114, 127, 196, 204, 218, 257, 260]
ids = [771, 114, 1434, 1052, 879, 727, 1396, 1007, 115, 328, ...]

====================================================================================

UMI info for barcode CTGCTGTTCGATAGAA-1 contig 1 = TGAGCGCAGA...
umi CACAGTCGCG = 41 reads: +22 -18 +179 -1 +171 non-validated
umi CACAGTCGCT = 201 reads: +391 validated
umi CCAGCCCTAC = 215 reads: +391 validated
umi CTATAGTGCA = 112 reads: +391 validated
umi CTGAATCGCT = 258 reads: +391 validated
umi GCTCTTCCTA = 200 reads: +195 -2XX +194 invalidated
umi TAGTATTTAT = 252 reads: +391 validated
umi TTCGATCATA = 128 reads: +391 validated

GOOD CONTIGS

TIG 1[bases=638]
0-36 ==> 0-36 on |330|IGLV1-51|5'UTR| [len=36] (mis=0)
36-389 ==> 0-353 on |331|IGLV1-51|L-REGION+V-REGION| [len=353] (mis=2)
389-427 ==> 0-38 on |309|IGLJ1|J-REGION| [len=38] (mis=0)
427-638 ==> 0-211 on |305|IGLC1|C-REGION| [len=317] (mis=0)
junction support: 8 umis using 213 reads
cdr3 = CGTWDSSLSAGYVF at 357, score = 7 + 8
umis assigned: [114, 115, 328, 727, 804, 1007, 1149, 1396]
of which 8 are surviving nonsolos
reads assigned: 1385
start codons at 36, 190, 241, 365, 391, 559
confident = true

REJECT CONTIGS

TIG 1[bases=631]
0-164 ==> 5-169 on rc of segment before IGKV3-11 exon 1 [len=169] (mis=0)
161-460 ==> 46-345 on |279|IGKV3-11|L-REGION+V-REGION| [len=345] (mis=0)
457-495 ==> 0-38 on |217|IGKJ5|J-REGION| [len=38] (mis=2)
495-631 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=1)
umis assigned: [771, 879, 1052]
of which 3 are surviving nonsolos
reads assigned: 194
start codons at 6, 131, 320, 323, 537
confident = false
did not find CDR3
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.164 = CTGCTGTTCTTGAGGT-1

using 811 reads

====================================================================================

graph has 206 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[2, 807]
surviving nonsolo ucounts = 1[807]
ids = [3]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.165 = CTGCTGTTCTTGCCGT-1

using 31 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 2[3, 23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.167 = CTGCTGTTCTTGTACT-1

using 254 reads

====================================================================================

graph has 84 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 3[2^2, 246]
surviving nonsolo ucounts = 1[246]
ids = [3]

====================================================================================

UMI info for barcode CTGCTGTTCTTGTACT-1 contig 1 = GGAGGAACTG...
umi GGCTCAGACT = 248 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=552]
0-34 ==> 13-47 on |278|IGKV3-11|5'UTR| [len=47] (mis=0)
34-379 ==> 0-345 on |279|IGKV3-11|L-REGION+V-REGION| [len=345] (mis=3)
379-416 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=0)
416-552 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 29 reads
cdr3 = CQQRSNWPLTF at 355, score = 9 + 9
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 244
start codons at 34, 242, 458
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.168 = CTGCTGTTCTTGTTTG-1

using 205 reads

====================================================================================

graph has 64 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 3[2, 3, 194]
surviving nonsolo ucounts = 1[194]
ids = [4]

====================================================================================

REJECT CONTIGS

TIG 1[bases=502]
0-64 ==> 0-64 on |66|IGHV1-2|5'UTR| [len=64] (mis=1)
2-82 ==> 10060-10140 on rc of segment before IGHV1-18 exon 2 [len=10140] (mis=10)
64-221 ==> 0-157 on |67|IGHV1-2|L-REGION+V-REGION| [len=353] (mis=1)
221-415 ==> 159-353 on |67|IGHV1-2|L-REGION+V-REGION| [len=353] (mis=3) [SHIFT!]
438-486 ==> 0-48 on |55|IGHJ4|J-REGION| [len=48] (mis=6)
cdr3 = CARSLVSYSSSWIFDYW at 404, score = 8 + 7
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 191
start codons at 64, 260, 265, 297, 326, 359
confident = false
frameshifted full length stopped transcript of length 502
VJ delta = 10
not full
not full
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.178 = CTGGTCTAGAGAACAG-1

using 380 reads

====================================================================================

graph has 130 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 2[156, 221]
surviving nonsolo ucounts = 2[156, 221]
ids = [0, 2]

====================================================================================

UMI info for barcode CTGGTCTAGAGAACAG-1 contig 1 = GGGAGGAACT...
umi AGAGATCATT = 158 reads: +372 -1 +9 non-validated
umi CCAGGTGCAG = 221 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=553]
0-35 ==> 12-47 on |278|IGKV3-11|5'UTR| [len=47] (mis=0)
35-380 ==> 0-345 on |279|IGKV3-11|L-REGION+V-REGION| [len=345] (mis=14)
379-417 ==> 0-38 on |215|IGKJ3|J-REGION| [len=38] (mis=7)
417-553 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 2 umis using 50 reads
cdr3 = CQQRRTWPPAF at 356, score = 9 + 6
umis assigned: [0, 2]
of which 2 are surviving nonsolos
reads assigned: 373
start codons at 35, 84, 240, 459
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.185 = CTGGTCTAGCACAGGT-1

using 207 reads

====================================================================================

graph has 61 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 1[200]
surviving nonsolo ucounts = 1[200]
ids = [1]

====================================================================================

UMI info for barcode CTGGTCTAGCACAGGT-1 contig 1 = GGGCCTCAGG...
umi ATTTATTTGA = 189 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=525]
0-35 ==> 17-52 on |348|IGLV3-1|5'UTR| [len=52] (mis=0)
35-381 ==> 0-346 on |349|IGLV3-1|L-REGION+V-REGION| [len=346] (mis=3)
379-417 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=1)
417-525 ==> 0-108 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 26 reads
cdr3 = CQAWDSSTAEVVF at 350, score = 6 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 189
start codons at 35, 40, 96, 183, 329, 333
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.188 = CTGGTCTAGCCAGGAT-1

using 833 reads

====================================================================================

graph has 278 edges initially, 4 edges after simplification

total ucounts = 11
nonsolo ucounts = 5[2, 3, 4, 11, 807]
surviving nonsolo ucounts = 2[11, 807]
ids = [4, 0]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.189 = CTGGTCTAGCCCAACC-1

using 102 reads

====================================================================================

graph has 56 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 3[2, 3, 89]
surviving nonsolo ucounts = 1[89]
ids = [1]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.191 = CTGGTCTAGCCGCCTA-1

using 413 reads

====================================================================================

graph has 260 edges initially, 2 edges after simplification

total ucounts = 26
nonsolo ucounts = 14[2^5, 3^3, 4, 5^2, 7, 8, 353]
surviving nonsolo ucounts = 1[353]
ids = [4]

====================================================================================

UMI info for barcode CTGGTCTAGCCGCCTA-1 contig 1 = ATCAGTCCCA...
umi CAAGCAGTCG = 350 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=547]
0-23 ==> 4-27 on |222|IGKV1-17|5'UTR| [len=27] (mis=0)
23-374 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
373-411 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
411-547 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 50 reads
cdr3 = CLQYSSSPWTF at 350, score = 9 + 8
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 344
start codons at 23, 29, 98, 234, 453
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.195 = CTGGTCTAGCTCCTTC-1

using 277 reads

====================================================================================

graph has 116 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[2, 4, 269]
surviving nonsolo ucounts = 1[269]
ids = [2]

====================================================================================

UMI info for barcode CTGGTCTAGCTCCTTC-1 contig 1 = AGTCAGTCTC...
umi TAATGACCTC = 250 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=488]
24-377 ==> 0-353 on |253|IGKV1D-39|L-REGION+V-REGION| [len=353] (mis=10)
375-412 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=1)
412-488 ==> 0-76 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 49 reads
cdr3 = CQQSYTTLLTF at 351, score = 9 + 9
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 245
start codons at 24, 30, 86, 99, 235, 454
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.199 = CTGGTCTAGGAGTTTA-1

using 471 reads

====================================================================================

graph has 164 edges initially, 2 edges after simplification

total ucounts = 13
nonsolo ucounts = 4[2^2, 3, 455]
surviving nonsolo ucounts = 1[455]
ids = [8]

====================================================================================

UMI info for barcode CTGGTCTAGGAGTTTA-1 contig 1 = ATCAGTCCCA...
umi GCCATTTTTG = 456 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=547]
0-23 ==> 4-27 on |222|IGKV1-17|5'UTR| [len=27] (mis=0)
23-374 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
373-411 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
411-547 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 76 reads
cdr3 = CLQYSSSPWTF at 350, score = 9 + 8
umis assigned: [8]
of which 1 are surviving nonsolos
reads assigned: 453
start codons at 23, 29, 98, 234, 453
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.213 = CTGGTCTAGTGCAAGC-1

using 328 reads

====================================================================================

graph has 106 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[328]
surviving nonsolo ucounts = 1[328]
ids = [0]

====================================================================================

UMI info for barcode CTGGTCTAGTGCAAGC-1 contig 1 = TGGGGAGGAA...
umi GCGACTCCAA = 333 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=555]
0-37 ==> 60-97 on |280|IGKV3-15|5'UTR| [len=97] (mis=0)
37-382 ==> 0-345 on |281|IGKV3-15|L-REGION+V-REGION| [len=345] (mis=12)
381-419 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=2)
419-555 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 54 reads
cdr3 = CLQYYNWPRTF at 358, score = 9 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 326
start codons at 37, 92, 106, 242, 461
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.222 = CTGGTCTCAAGTAGTA-1

using 30 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[2, 4, 5, 17]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.224 = CTGGTCTCAAGTCTGT-1

using 16 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[3, 13]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.225 = CTGGTCTCAATAGCGG-1

using 571 reads

====================================================================================

graph has 450 edges initially, 2 edges after simplification

total ucounts = 126
nonsolo ucounts = 35[2^16, 3^5, 4^3, 5^2, 6, 7, 8^2, 9^2, 10, 13, 341]
surviving nonsolo ucounts = 1[341]
ids = [74]

====================================================================================

UMI info for barcode CTGGTCTCAATAGCGG-1 contig 1 = GGAGGAACTG...
umi GCCTTTTCCT = 317 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=510]
0-34 ==> 13-47 on |278|IGKV3-11|5'UTR| [len=47] (mis=0)
34-379 ==> 0-345 on |279|IGKV3-11|L-REGION+V-REGION| [len=345] (mis=14)
378-416 ==> 0-38 on |215|IGKJ3|J-REGION| [len=38] (mis=7)
416-510 ==> 0-94 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 47 reads
cdr3 = CQQRRTWPPAF at 355, score = 9 + 6
umis assigned: [74]
of which 1 are surviving nonsolos
reads assigned: 314
start codons at 34, 83, 239, 458
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.227 = CTGGTCTCAATCCGAT-1

using 24 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.233 = CTGGTCTCACCGAATT-1

using 306 reads

====================================================================================

graph has 94 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 2[2, 300]
surviving nonsolo ucounts = 1[300]
ids = [2]

====================================================================================

REJECT CONTIGS

TIG 1[bases=485]
0-35 ==> 17-52 on |282|IGKV3-20|5'UTR| [len=52] (mis=0)
0-35 ==> 5965-6000 on rc of segment after IGKV3-20 exon 1 [len=6000] (mis=0)
35-383 ==> 0-348 on |283|IGKV3-20|L-REGION+V-REGION| [len=348] (mis=4)
381-411 ==> 0-30 on |214|IGKJ2|J-REGION| [len=39] (mis=5)
419-485 ==> 0-66 on |212|IGKC|C-REGION| [len=320] (mis=0)
cdr3 = CQQYGSSPVTF at 359, score = 9 + 7
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 273
start codons at 35, 243, 246, 369, 461
confident = false
not full
VJ delta = 15
not full
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.235 = CTGGTCTCACGAGGTA-1

using 202 reads

====================================================================================

graph has 100 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 4[2^2, 3, 189]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.246 = CTGGTCTCAGATGGCA-1

using 233 reads

====================================================================================

graph has 86 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 3[2^2, 221]
surviving nonsolo ucounts = 1[221]
ids = [8]

====================================================================================

UMI info for barcode CTGGTCTCAGATGGCA-1 contig 1 = GCTCTGCTTC...
umi GTTTATACGA = 208 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=606]
0-51 ==> 0-51 on |320|IGLV1-40|5'UTR| [len=51] (mis=0)
51-407 ==> 0-356 on |321|IGLV1-40|L-REGION+V-REGION| [len=356] (mis=5)
401-439 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=3)
439-606 ==> 0-167 on |307|IGLC3|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 31 reads
cdr3 = CQSYDSSLREVF at 375, score = 8 + 8
umis assigned: [8]
of which 1 are surviving nonsolos
reads assigned: 207
start codons at 51, 205, 208, 259, 358, 385
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.250 = CTGGTCTCAGCCTGTG-1

using 640 reads

====================================================================================

graph has 172 edges initially, 12 edges after simplification

total ucounts = 11
nonsolo ucounts = 4[2^2, 255, 374]
surviving nonsolo ucounts = 2[255, 374]
ids = [2, 8]

====================================================================================

UMI info for barcode CTGGTCTCAGCCTGTG-1 contig 1 = AGGAGTCAGT...
umi ATCGTTGATC = 259 reads: +388 validated
umi TATAATGGTC = 366 reads: +41 -1XX +10 -1XX +3 -2XX +1 -6XX +2 -291X +2 -3XX +2 -9XX +1 -1XX +1 -3XX +1 -1XX +2 -2XX +1 -1XX invalidated

GOOD CONTIGS

TIG 1[bases=551]
27-380 ==> 0-353 on |253|IGKV1D-39|L-REGION+V-REGION| [len=353] (mis=11)
378-415 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=0)
415-551 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 49 reads
cdr3 = CQQSYTTLLTF at 354, score = 9 + 9
umis assigned: [2, 8]
of which 2 are surviving nonsolos
reads assigned: 617
start codons at 27, 33, 89, 102, 238, 457
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.254 = CTGGTCTCAGCTCCGA-1

using 134 reads

====================================================================================

graph has 76 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[134]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.263 = CTGGTCTCAGTGACAG-1

using 62 reads

====================================================================================

graph has 66 edges initially, 2 edges after simplification

total ucounts = 13
nonsolo ucounts = 6[2^3, 3, 7, 39]
surviving nonsolo ucounts = 1[39]
ids = [8]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.277 = CTGGTCTGTAATAGCA-1

using 236 reads

====================================================================================

graph has 94 edges initially, 2 edges after simplification

total ucounts = 12
nonsolo ucounts = 1[225]
surviving nonsolo ucounts = 1[225]
ids = [6]

====================================================================================

UMI info for barcode CTGGTCTGTAATAGCA-1 contig 1 = CTGGGCCTCA...
umi GCCTCTCCAC = 211 reads: +379 validated

GOOD CONTIGS

TIG 1[bases=567]
0-37 ==> 15-52 on |348|IGLV3-1|5'UTR| [len=52] (mis=0)
37-371 ==> 0-334 on |349|IGLV3-1|L-REGION+V-REGION| [len=346] (mis=11)
378-416 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=1)
416-567 ==> 0-151 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 40 reads
cdr3 = CQAWDSTTDWVF at 352, score = 6 + 8
umis assigned: [6]
of which 1 are surviving nonsolos
reads assigned: 208
start codons at 37, 42, 98, 185, 331, 335
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.280 = CTGGTCTGTACCAGTT-1

using 1532 reads

====================================================================================

graph has 2532 edges initially, 6 edges after simplification

total ucounts = 659
nonsolo ucounts = 346[2^141, 3^98, 4^43, 5^30, 6^18, 7^7, 8^3, 9^3, 10, 18, 85]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.290 = CTGGTCTGTATTCGTG-1

using 1013 reads

====================================================================================

graph has 1000 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[1012]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.296 = CTGGTCTGTCGACTGC-1

using 15 reads

====================================================================================

graph has 6 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.299 = CTGGTCTGTCTAGCGC-1

using 24 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.307 = CTGGTCTGTCTTGCGG-1

using 247 reads

====================================================================================

graph has 232 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 1[244]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.314 = CTGGTCTGTGGGTCAA-1

using 287 reads

====================================================================================

graph has 82 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 3[2, 5, 272]
surviving nonsolo ucounts = 1[272]
ids = [2]

====================================================================================

UMI info for barcode CTGGTCTGTGGGTCAA-1 contig 1 = GGGAGGAACT...
umi CATGCGCGTT = 274 reads: +379 validated

GOOD CONTIGS

TIG 1[bases=550]
0-35 ==> 12-47 on |278|IGKV3-11|5'UTR| [len=47] (mis=0)
35-380 ==> 0-345 on |279|IGKV3-11|L-REGION+V-REGION| [len=345] (mis=3)
377-414 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=0)
414-550 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 43 reads
cdr3 = CQQRSNRLTF at 356, score = 9 + 9
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 271
start codons at 35, 240, 243, 456
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.316 = CTGGTCTGTGTTGGGA-1

using 227 reads

====================================================================================

graph has 108 edges initially, 2 edges after simplification

total ucounts = 12
nonsolo ucounts = 4[2^2, 3, 212]
surviving nonsolo ucounts = 1[212]
ids = [2]

====================================================================================

UMI info for barcode CTGGTCTGTGTTGGGA-1 contig 1 = GGGGCTGTCT...
umi ATTGAAACAT = 205 reads: +433 validated

GOOD CONTIGS

TIG 1[bases=457]
17-388 ==> 0-371 on |186|IGHV4-34|L-REGION+V-REGION| [len=371] (mis=15)
414-450 ==> 13-49 on |56|IGHJ5|J-REGION| [len=49] (mis=1)
junction support: 1 umis using 26 reads
cdr3 = CATLDIVAPGSDSW at 377, score = 9 + 7
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 203
start codons at 17, 38, 82
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.329 = CTGGTCTGTTGTTTGG-1

using 338 reads

====================================================================================

graph has 114 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 6[2^4, 3, 322]
surviving nonsolo ucounts = 1[322]
ids = [10]

====================================================================================

UMI info for barcode CTGGTCTGTTGTTTGG-1 contig 1 = AGGAGTCAGA...
umi TTAAGTTTAT = 308 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=495]
0-27 ==> 20-47 on |218|IGKV1-12|5'UTR| [len=47] (mis=0)
27-378 ==> 0-351 on |219|IGKV1-12|L-REGION+V-REGION| [len=351] (mis=0)
378-415 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=0)
415-495 ==> 0-80 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 37 reads
cdr3 = CQQANSFPLTF at 354, score = 9 + 9
umis assigned: [10]
of which 1 are surviving nonsolos
reads assigned: 305
start codons at 27, 33, 89, 102, 238, 457
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.333 = CTGGTCTTCAAACGGG-1

using 14752 reads

====================================================================================

graph has 5733 edges initially, 52 edges after simplification

total ucounts = 793
nonsolo ucounts = 359[2^133, 3^64, 4^42, 5^23, 6^10, 7^6, 8^6, 9, 10, 11^2, 12, 14, 17, 22, 53, 55, 59, 63, 65, 70^2, 80, 85, 98, 119, 128, 137, 138, 147^2, 150^2, 155, 157, 159, 170, 172, 173, 174, 175, 176, 182, 188, 190^2, 194, 196, 198, 199^3, 204, 206, 207, 210^2, 212, 216, 217, 224, 227, 238, 246, 247^2, 251, 263, 268, 271^3, 274, 277, 284, 287, 295, 302, 316, 346, 392, 581]
surviving nonsolo ucounts = 65[2, 55, 59, 63, 65, 70, 85, 98, 119, 128, 137, 138, 147^2, 150^2, 155, 157, 159, 170, 172, 173, 174, 175, 176, 182, 188, 190^2, 194, 196, 198, 199^3, 204, 206, 207, 210^2, 212, 216, 217, 224, 227, 238, 246, 247^2, 251, 263, 268, 271^3, 274, 277, 284, 287, 295, 302, 316, 346, 392, 581]
ids = [153, 129, 481, 379, 561, 787, 250, 128, 391, 8, ...]

====================================================================================

UMI info for barcode CTGGTCTTCAAACGGG-1 contig 1 = TGGGGACCCA...
umi AAATGTCACT = 141 reads: +421 validated
umi ACTATCTCCT = 211 reads: +421 validated
umi ACTTTGACTG = 172 reads: +406 -15 non-validated
umi AGAGGAGCTT = 97 reads: +419 -1 +1 non-validated
umi AGATACGTTT = 48 reads: +382 -39 non-validated
umi ATAATTCCAC = 146 reads: +421 validated
umi ATCATTCCTA = 5 reads: -362X +2 -3XX +1 -4XX +2 -1XX +1 -2XX +2 -1XX +1 -4XX +35 invalidated
umi CACTTTTTAC = 84 reads: +337 -1X +4 -1 +10 -1 +1 -1 +50 -1 +5 -9 invalidated
umi CAGGTACCCC = 248 reads: +421 validated
umi CCCCGCACTC = 201 reads: +421 validated
umi CGTACACTAG = 272 reads: +421 validated
umi CTCCGTGGGG = 57 reads: +392 -2 +27 non-validated
umi CTGTCAGTTA = 117 reads: +406 -1 +14 non-validated
umi GATAGCTTCT = 167 reads: +421 validated
umi GCAGGCTGCG = 137 reads: +397 -24 non-validated
umi GCGCCGGTCT = 197 reads: +415 -6 non-validated
umi GTAATCAGTG = 307 reads: +421 validated
umi GTCCCCATCT = 312 reads: +421 validated
umi GTCGCATTGG = 253 reads: +421 validated
umi TAAATTACAA = 246 reads: +418 -2 +1 non-validated
umi TACTTGTGGC = 317 reads: +421 validated
umi TAGGGCTAGC = 6 reads: -351X +1 -3X +1 -6XX +2 -3XX +1 -4XX +2 -1XX +1 -2XX +2 -1XX +1 -4XX +35 invalidated
umi TCATGGACAG = 157 reads: +421 validated
umi TCGGTAGTTA = 143 reads: +421 validated
umi TCGTGAATAT = 275 reads: +421 validated
umi TGCTTCTCAC = 241 reads: +421 validated
umi TTAGATTTCT = 286 reads: +421 validated

UMI info for barcode CTGGTCTTCAAACGGG-1 contig 2 = GGGGGCTGGG...
umi AAATATCTTG = 127 reads: +394 validated
umi AACATCATAG = 136 reads: +394 validated
umi AATAGCGCCG = 187 reads: +394 validated
umi AATCGCGCTC = 258 reads: +394 validated
umi AATTTCCCCT = 269 reads: +394 validated
umi ACATTCACTA = 210 reads: +394 validated
umi ACCTCCGCCG = 190 reads: +394 validated
umi ACGTAATATC = 228 reads: +394 validated
umi ACTATTTCAG = 214 reads: +394 validated
umi ATACGAATGA = 189 reads: +17 -2XX +157 -1XX +35 -1XX +139 -4X +1 -2XX +3 -1XX +31 invalidated
umi ATCACTAGGA = 247 reads: +394 validated
umi ATGGCACACC = 195 reads: +394 validated
umi ATTTCCGGGC = 209 reads: +394 validated
umi CATTTGTCAC = 173 reads: +394 validated
umi GACACCGCCA = 205 reads: +394 validated
umi GACAGGCCTT = 167 reads: +394 validated
umi GACCGCCAAT = 181 reads: +188 -2XX +204 invalidated
umi GCGCCTTCTT = 188 reads: +394 validated
umi GGACCCATGG = 194 reads: +394 validated
umi GTATGCTCTC = 129 reads: +33 -1 +1 -1XX +1 -6XX +317 -14 +20 invalidated
umi GTGCAGTTGG = 205 reads: +394 validated
umi GTTGTTATTT = 66 reads: +394 validated
umi TAAGAGTCTT = 195 reads: +394 validated
umi TAGCGGCTTT = 211 reads: +394 validated
umi TCTGATAGCA = 394 reads: +394 validated
umi TGTACCGATA = 159 reads: +394 validated
umi TTAGCGGCGT = 216 reads: +394 validated
umi TTCCAGAGCA = 148 reads: +394 validated
umi TTTCTGTGGG = 196 reads: +394 validated
umi TTTTCTCCAT = 68 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=551]
0-59 ==> 0-59 on |64|IGHV1-18|5'UTR| [len=59] (mis=5)
59-412 ==> 0-353 on |65|IGHV1-18|L-REGION+V-REGION| [len=353] (mis=1)
432-480 ==> 0-48 on |55|IGHJ4|J-REGION| [len=48] (mis=4)
480-551 ==> 0-71 on |60|IGHM|C-REGION| [len=1358] (mis=0)
junction support: 19 umis using 322 reads
cdr3 = CARDLRLRGSNYFGYW at 401, score = 8 + 7
umis assigned: [9, 100, 116, 128, 129, 166, 181, 250, 258, 301] and 17 others
of which 25 are surviving nonsolos
reads assigned: 4773
start codons at 59, 210, 257, 262, 279, 294, 323, 356
confident = true

TIG 2[bases=650]
45-406 ==> 0-361 on |339|IGLV2-14|L-REGION+V-REGION| [len=361] (mis=9)
401-439 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=0)
439-650 ==> 0-211 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 28 umis using 765 reads
cdr3 = CSSYTSSSTPHVVF at 369, score = 8 + 8
umis assigned: [8, 14, 43, 46, 55, 66, 82, 94, 104, 170] and 20 others
of which 30 are surviving nonsolos
reads assigned: 5651
start codons at 45, 202, 246, 253, 256, 400
confident = true

REJECT CONTIGS

TIG 1[bases=556]
0-63 ==> 5937-6000 on segment before IGKV2D-40 exon 1 [len=6000] (mis=2)
23-69 ==> 3411-3457 on segment before IGKV2OR2-2 exon 1 [len=3837] (mis=2)
30-390 ==> 0-360 on |275|IGKV2D-29|L-REGION+V-REGION| [len=360] (mis=7)
382-420 ==> 0-38 on |217|IGKJ5|J-REGION| [len=38] (mis=5)
420-556 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
umis assigned: [217, 341, 362, 481, 585, 593, 599, 742]
of which 8 are surviving nonsolos
reads assigned: 2192
start codons at 30, 63, 99, 187, 250, 349, 369, 462
confident = false
did not find CDR3
now this is a cell
paired!

GACGACACGGCCGTGTATTACTGTGCGAGAGATCTCAGACTACGGGGATCTAATTATTTTGGGTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG <==> CTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATATACAAGCAGCAGCACTCCCCATGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.337 = CTGGTCTTCAACGGGA-1

using 2816 reads

====================================================================================

graph has 3640 edges initially, 30 edges after simplification

total ucounts = 1360
nonsolo ucounts = 562[2^241, 3^120, 4^72, 5^51, 6^27, 7^19, 8^10, 9^8, 10^3, 11^3, 12^3, 13, 15, 16, 17, 26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.341 = CTGGTCTTCACCTCGT-1

using 689 reads

====================================================================================

graph has 208 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 3[3, 5, 674]
surviving nonsolo ucounts = 1[674]
ids = [8]

====================================================================================

UMI info for barcode CTGGTCTTCACCTCGT-1 contig 1 = GAGGAGTCAG...
umi TTACTTAGTC = 680 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=552]
28-381 ==> 0-353 on |253|IGKV1D-39|L-REGION+V-REGION| [len=353] (mis=16)
378-416 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=2)
416-552 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 92 reads
cdr3 = CQQSYSTPWQF at 355, score = 8 + 7
umis assigned: [8]
of which 1 are surviving nonsolos
reads assigned: 666
start codons at 28, 34, 90, 103, 239, 458
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.345 = CTGGTCTTCAGCATGT-1

using 18 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.356 = CTGGTCTTCCGAATGT-1

using 316 reads

====================================================================================

graph has 136 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 4[2^2, 5, 301]
surviving nonsolo ucounts = 1[301]
ids = [7]

====================================================================================

UMI info for barcode CTGGTCTTCCGAATGT-1 contig 1 = AGAGCTCTGG...
umi TTCTATCCCT = 278 reads: +385 validated

GOOD CONTIGS

TIG 1[bases=521]
0-44 ==> 8-52 on |282|IGKV3-20|5'UTR| [len=52] (mis=0)
44-392 ==> 0-348 on |283|IGKV3-20|L-REGION+V-REGION| [len=348] (mis=17)
391-429 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
429-521 ==> 0-92 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 36 reads
cdr3 = CQQYGSSPWTF at 368, score = 9 + 8
umis assigned: [7]
of which 1 are surviving nonsolos
reads assigned: 277
start codons at 44, 252, 378, 471
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.360 = CTGGTCTTCCTAAGTG-1

using 328 reads

====================================================================================

graph has 104 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 2[2, 319]
surviving nonsolo ucounts = 1[319]
ids = [0]

====================================================================================

UMI info for barcode CTGGTCTTCCTAAGTG-1 contig 1 = GAGTCAGTCT...
umi AAGCAGGTTA = 321 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=549]
25-378 ==> 0-353 on |253|IGKV1D-39|L-REGION+V-REGION| [len=353] (mis=3)
374-413 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=3)
413-549 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 43 reads
cdr3 = CQQSYSTPYTF at 352, score = 9 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 315
start codons at 25, 31, 87, 100, 236, 455
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.369 = CTGGTCTTCGTAGATC-1

using 291 reads

====================================================================================

graph has 120 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 7[2^2, 3, 4^2, 5, 268]
surviving nonsolo ucounts = 1[268]
ids = [5]

====================================================================================

UMI info for barcode CTGGTCTTCGTAGATC-1 contig 1 = GGAATCAGTC...
umi TAAGGATAGT = 270 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=550]
0-26 ==> 1-27 on |222|IGKV1-17|5'UTR| [len=27] (mis=0)
26-377 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
376-414 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
414-550 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 46 reads
cdr3 = CLQYSSSPWTF at 353, score = 9 + 8
umis assigned: [5]
of which 1 are surviving nonsolos
reads assigned: 267
start codons at 26, 32, 101, 237, 456
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.392 = CTGGTCTTCTTTCCTC-1

using 99 reads

====================================================================================

graph has 132 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[99]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.395 = CTGTGCTAGAAGATTC-1

using 1505 reads

====================================================================================

graph has 2149 edges initially, 14 edges after simplification

total ucounts = 628
nonsolo ucounts = 309[2^130, 3^70, 4^41, 5^23, 6^19, 7^7, 8^8, 9^2, 10^4, 11^2, 14, 18, 98]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.398 = CTGTGCTAGACAGGCT-1

using 414 reads

====================================================================================

graph has 186 edges initially, 4 edges after simplification

total ucounts = 10
nonsolo ucounts = 6[2^2, 3, 5, 57, 341]
surviving nonsolo ucounts = 2[57, 341]
ids = [0, 4]

====================================================================================

UMI info for barcode CTGTGCTAGACAGGCT-1 contig 1 = TGGGGAGGAA...
umi ATCACGTATA = 338 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=556]
32-383 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
382-420 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
420-556 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 45 reads
cdr3 = CLQYSSSPWTF at 359, score = 9 + 8
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 333
start codons at 32, 38, 107, 243, 462
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.403 = CTGTGCTAGCAATCTC-1

using 197 reads

====================================================================================

graph has 88 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 2[2, 189]
surviving nonsolo ucounts = 1[189]
ids = [5]

====================================================================================

UMI info for barcode CTGTGCTAGCAATCTC-1 contig 1 = GGAGGAATCA...
umi GCATTTTGCA = 167 reads: +388 validated
umi TCATTTTGCC = 1 reads: -288 +3 -1 +2 -3X +1 -1 +2 -3 +3 -1 +3 -2 +4 -1 +16 -1 +5 -1 +2 -45 invalidated

GOOD CONTIGS

TIG 1[bases=507]
29-380 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
379-417 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
417-507 ==> 0-90 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 35 reads
cdr3 = CLQYSSSPWTF at 356, score = 9 + 8
umis assigned: [5, 6]
of which 1 are surviving nonsolos
reads assigned: 168
start codons at 29, 35, 104, 240, 459
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.410 = CTGTGCTAGCGAAGGG-1

using 149 reads

====================================================================================

graph has 138 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[148]
surviving nonsolo ucounts = 1[148]
ids = [0]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.411 = CTGTGCTAGCGTAGTG-1

using 93 reads

====================================================================================

graph has 132 edges initially, 2 edges after simplification

total ucounts = 39
nonsolo ucounts = 19[2^9, 3^4, 4^2, 5^2, 9, 16]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.419 = CTGTGCTAGGCCCTTG-1

using 233 reads

====================================================================================

graph has 96 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 3[2, 3, 228]
surviving nonsolo ucounts = 1[228]
ids = [2]

====================================================================================

UMI info for barcode CTGTGCTAGGCCCTTG-1 contig 1 = TGAGCGCAGA...
umi TTCGTGGATA = 216 reads: +391 validated

GOOD CONTIGS

TIG 1[bases=576]
0-36 ==> 0-36 on |330|IGLV1-51|5'UTR| [len=36] (mis=0)
36-389 ==> 0-353 on |331|IGLV1-51|L-REGION+V-REGION| [len=353] (mis=12)
389-427 ==> 0-38 on |309|IGLJ1|J-REGION| [len=38] (mis=1)
427-576 ==> 0-149 on |305|IGLC1|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 34 reads
cdr3 = CATWETSLSAPYVF at 357, score = 6 + 8
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 214
start codons at 36, 190, 365, 391, 559
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.425 = CTGTGCTAGTCTCCTC-1

using 264 reads

====================================================================================

graph has 116 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 1[259]
surviving nonsolo ucounts = 1[259]
ids = [5]

====================================================================================

UMI info for barcode CTGTGCTAGTCTCCTC-1 contig 1 = TGAGCGCAGA...
umi GTAATTCTCC = 244 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=518]
0-36 ==> 0-36 on |330|IGLV1-51|5'UTR| [len=36] (mis=0)
36-389 ==> 0-353 on |331|IGLV1-51|L-REGION+V-REGION| [len=353] (mis=11)
386-424 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=3)
424-518 ==> 0-94 on |307|IGLC3|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 39 reads
cdr3 = CGTWDSSLSGGVF at 357, score = 7 + 8
umis assigned: [5]
of which 1 are surviving nonsolos
reads assigned: 239
start codons at 36, 190, 197, 241, 250, 365
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.426 = CTGTGCTAGTGATCGG-1

using 215 reads

====================================================================================

graph has 84 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 4[2^2, 3, 204]
surviving nonsolo ucounts = 1[204]
ids = [6]

====================================================================================

REJECT CONTIGS

TIG 1[bases=423]
1-59 ==> 4685-4743 on rc of segment before IGHVII-28-1 exon 1 [len=4743] (mis=3)
59-82 ==> 0-23 on |192|IGHV4-4|L-REGION+V-REGION| [len=353] (mis=0)
196-241 ==> 308-353 on |192|IGHV4-4|L-REGION+V-REGION| [len=353] (mis=4)
287-327 ==> 21-61 on |58|IGHJ6|J-REGION| [len=61] (mis=1)
327-423 ==> 0-96 on |1|IGHA1|C-REGION| [len=1058] (mis=0)
cdr3 = CARVLTSGYYVSSSNYFKIDVW at 230, score = 9 + 6
umis assigned: [6]
of which 1 are surviving nonsolos
reads assigned: 131
start codons at 15, 59, 381
confident = false
full length transcript of length 423
VJ delta = 192
delta too large
not full
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.427 = CTGTGCTAGTGGAGTC-1

using 186 reads

====================================================================================

graph has 106 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 1[180]
surviving nonsolo ucounts = 1[180]
ids = [1]

====================================================================================

UMI info for barcode CTGTGCTAGTGGAGTC-1 contig 1 = AGCACTAGAA...
umi CAGTCAAAGA = 166 reads: +418 validated

GOOD CONTIGS

TIG 1[bases=499]
0-60 ==> 20-80 on |123|IGHV3-30|5'UTR| [len=80] (mis=0)
60-411 ==> 0-351 on |124|IGHV3-30|L-REGION+V-REGION| [len=351] (mis=0)
419-450 ==> 0-31 on |14|IGHD2-2|D-REGION| [len=31] (mis=3)
448-478 ==> 16-46 on |54|IGHJ4|J-REGION| [len=46] (mis=0)
478-499 ==> 0-21 on |6|IGHD|C-REGION| [len=1289] (mis=0)
junction support: 1 umis using 20 reads
cdr3 = CAKERLGYCSSNSCY at 402, score = 9 + 5
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 161
start codons at 60, 211, 216, 274, 277, 295, 363
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.434 = CTGTGCTCAAAGGAAG-1

using 186 reads

====================================================================================

graph has 168 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 4[3, 4, 5, 171]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================

REJECT CONTIGS

TIG 1[bases=386]
60-77 ==> 3282-3299 on segment after IGHV1OR16-1 exon 2 [len=6000] (mis=0)
60-77 ==> 3279-3296 on segment after IGHV1OR16-2 exon 2 [len=6000] (mis=0)
304-340 ==> 10-46 on |54|IGHJ4|J-REGION| [len=46] (mis=0)
340-386 ==> 0-46 on |43|IGHG2|C-REGION| [len=977] (mis=0)
umis assigned: [5]
of which 0 are surviving nonsolos
reads assigned: 110
start codons at 88, 93, 172, 201, 240, 301
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.442 = CTGTGCTCAATGGAGC-1

using 5665 reads

====================================================================================

graph has 5108 edges initially, 58 edges after simplification

total ucounts = 1200
nonsolo ucounts = 568[2^205, 3^118, 4^73, 5^59, 6^28, 7^22, 8^19, 9^15, 10^10, 11^3, 12^2, 13, 14, 15, 20, 22, 192, 204, 289, 293, 304, 355, 363, 385, 447]
surviving nonsolo ucounts = 10[3, 192, 204, 289, 293, 304, 355, 363, 385, 447]
ids = [968, 1157, 156, 325, 519, 624, 790, 129, 787, 651]

====================================================================================

UMI info for barcode CTGTGCTCAATGGAGC-1 contig 1 = AAGCCCCCAG...
umi TTGATAGCCC = 178 reads: +448 validated

UMI info for barcode CTGTGCTCAATGGAGC-1 contig 2 = AGGAATCAGA...
umi ACCAACCGGG = 369 reads: +388 validated
umi ACCTGCGCGC = 206 reads: +388 validated
umi ATGAAAACCT = 290 reads: +388 validated
umi CCTTTCCAAG = 295 reads: +388 validated
umi CTCTCATTGG = 307 reads: +388 validated
umi CTTGGAGGAG = 449 reads: +388 validated
umi GGACCTGTGC = 360 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=508]
0-26 ==> 16-42 on |72|IGHV1-3|5'UTR| [len=42] (mis=0)
26-379 ==> 0-353 on |73|IGHV1-3|L-REGION+V-REGION| [len=353] (mis=11)
379-410 ==> 0-31 on |21|IGHD3-3|D-REGION| [len=31] (mis=5)
423-474 ==> 12-63 on |737|IGHJ6|J-REGION| [len=63] (mis=2)
474-508 ==> 0-34 on |1|IGHA1|C-REGION| [len=1058] (mis=0)
junction support: 1 umis using 21 reads
cdr3 = CASGLDDFWSGYYDRGYHNYGMNVW at 368, score = 9 + 7
umis assigned: [1157]
of which 1 are surviving nonsolos
reads assigned: 173
start codons at 26, 177, 224, 229, 323, 405, 431
confident = true

TIG 2[bases=551]
0-27 ==> 0-27 on |220|IGKV1-16|5'UTR| [len=27] (mis=0)
27-378 ==> 0-351 on |221|IGKV1-16|L-REGION+V-REGION| [len=351] (mis=9)
376-415 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=5)
415-551 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 7 umis using 328 reads
cdr3 = CQQYITHPYTF at 354, score = 9 + 8
umis assigned: [129, 156, 325, 519, 624, 651, 790]
of which 7 are surviving nonsolos
reads assigned: 2226
start codons at 27, 33, 89, 102, 238, 457
confident = true
now this is a cell
paired!

AGCGGGTTAGACGATTTTTGGAGTGGTTATTATGACCGGGGGTACCACAACTACGGTATGAACGTCTGGGGCCAAGGGACCACCGTCACCGTCTCCTCAG <==> ATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGCCAACAGTATATTACTCACCCGTATACTTTTGGCCAGGGGACCAAGCTAGAGATCAAAC

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.454 = CTGTGCTCAGAAGCAC-1

using 5069 reads

====================================================================================

graph has 5694 edges initially, 52 edges after simplification

total ucounts = 1698
nonsolo ucounts = 739[2^296, 3^164, 4^94, 5^62, 6^42, 7^24, 8^19, 9^11, 10^4, 11^8, 12^5, 13^3, 15^2, 17, 281, 344, 373, 397]
surviving nonsolo ucounts = 4[281, 344, 373, 397]
ids = [454, 1220, 1205, 1505]

====================================================================================

UMI info for barcode CTGTGCTCAGAAGCAC-1 contig 1 = GAGTCAGTCT...
umi ATCACAGCTG = 265 reads: -355 +1 -1X +6 -2XX +8 -1XX +3 -1XX +2 -1XX +4 -1XX +2 invalidated
umi GTTCACATCG = 375 reads: +388 validated
umi GTTTGTCCAC = 352 reads: +388 validated
umi TGCCTCTATA = 397 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=549]
0-25 ==> 22-47 on |230|IGKV1-39|5'UTR| [len=47] (mis=0)
25-376 ==> 0-351 on |231|IGKV1-39|L-REGION+V-REGION| [len=351] (mis=17)
375-413 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=4)
413-549 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 3 umis using 180 reads
cdr3 = CQQSYTSSWTF at 352, score = 9 + 8
umis assigned: [454, 1205, 1220, 1505]
of which 4 are surviving nonsolos
reads assigned: 1360
start codons at 25, 31, 87, 100, 236, 455
confident = true
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.459 = CTGTGCTCAGCTGCAC-1

using 975 reads

====================================================================================

graph has 360 edges initially, 30 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[263, 710]
surviving nonsolo ucounts = 2[263, 710]
ids = [2, 1]

====================================================================================

UMI info for barcode CTGTGCTCAGCTGCAC-1 contig 1 = GGCACAAGAG...
umi ATCAAGCGGA = 703 reads: -382X +1 -1XX +9 -1XX invalidated
umi GGCGAGCCCT = 265 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=639]
0-34 ==> 17-51 on |320|IGLV1-40|5'UTR| [len=51] (mis=0)
34-390 ==> 0-356 on |321|IGLV1-40|L-REGION+V-REGION| [len=356] (mis=12)
390-428 ==> 0-38 on |309|IGLJ1|J-REGION| [len=38] (mis=1)
428-639 ==> 0-211 on |305|IGLC1|C-REGION| [len=317] (mis=1)
junction support: 1 umis using 42 reads
cdr3 = CQSYDSSLSGLYVF at 358, score = 7 + 8
umis assigned: [1, 2]
of which 2 are surviving nonsolos
reads assigned: 929
start codons at 34, 188, 191, 242, 341, 368, 392
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.472 = CTGTGCTCATCGGACC-1

using 347 reads

====================================================================================

graph has 200 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 5[2^2, 5, 8, 326]
surviving nonsolo ucounts = 2[8, 326]
ids = [6, 2]

====================================================================================

UMI info for barcode CTGTGCTCATCGGACC-1 contig 1 = GAGCATCACC...
umi CGCCACCGCT = 327 reads: +424 validated
umi TATGCCTTCT = 9 reads: +14 -71 +30 -1 +25 -4 +56 -51 +7 -1 +9 -1 +9 -1 +28 -1 +76 -39 non-validated

GOOD CONTIGS

TIG 1[bases=557]
0-62 ==> 2-64 on |66|IGHV1-2|5'UTR| [len=64] (mis=1)
62-415 ==> 0-353 on |67|IGHV1-2|L-REGION+V-REGION| [len=353] (mis=7)
438-486 ==> 0-48 on |55|IGHJ4|J-REGION| [len=48] (mis=6)
486-557 ==> 0-71 on |60|IGHM|C-REGION| [len=1358] (mis=0)
junction support: 1 umis using 22 reads
cdr3 = CARSLVSYSSSWIFDYW at 404, score = 8 + 7
umis assigned: [2, 6]
of which 2 are surviving nonsolos
reads assigned: 328
start codons at 62, 260, 265, 297, 326, 359
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.475 = CTGTGCTCATGCAACT-1

using 513 reads

====================================================================================

graph has 206 edges initially, 4 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[242, 269]
surviving nonsolo ucounts = 2[242, 269]
ids = [2, 0]

====================================================================================

UMI info for barcode CTGTGCTCATGCAACT-1 contig 1 = GGGCTGGGGT...
umi AACATCATCC = 272 reads: +391 validated

UMI info for barcode CTGTGCTCATGCAACT-1 contig 2 = GGGGAGGAAT...
umi CTGCAGTGGC = 214 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=645]
43-396 ==> 0-353 on |339|IGLV2-14|L-REGION+V-REGION| [len=361] (mis=3)
396-434 ==> 0-38 on |309|IGLJ1|J-REGION| [len=38] (mis=4)
434-645 ==> 0-211 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 49 reads
cdr3 = CSSYTSSSTLRVF at 367, score = 8 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 266
start codons at 43, 200, 244, 251, 254
confident = false

TIG 2[bases=512]
31-382 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
381-419 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
419-512 ==> 0-93 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 41 reads
cdr3 = CLQYSSSPWTF at 358, score = 9 + 8
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 211
start codons at 31, 37, 106, 242, 461
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.491 = CTGTGCTGTAGATTAG-1

using 1504 reads

====================================================================================

graph has 2086 edges initially, 26 edges after simplification

total ucounts = 682
nonsolo ucounts = 355[2^163, 3^84, 4^40, 5^28, 6^20, 7^9, 8^3, 9, 10^2, 11, 12^2, 14^2]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.496 = CTGTGCTGTATCACCA-1

using 188 reads

====================================================================================

graph has 110 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 1[184]
surviving nonsolo ucounts = 1[184]
ids = [1]

====================================================================================

REJECT CONTIGS

TIG 1[bases=380]
0-292 ==> 71-363 on |296|IGKV4-1|L-REGION+V-REGION| [len=363] (mis=1)
291-329 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=2)
329-380 ==> 0-51 on |212|IGKC|C-REGION| [len=320] (mis=0)
cdr3 = CQQYYSTPRTF at 268, score = 9 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 152
start codons at 251, 371
confident = false
not full
VJ delta = 14
not full
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.503 = CTGTGCTGTCATCCCT-1

using 82 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[82]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.522 = CTGTGCTGTGAGGGTT-1

using 246 reads

====================================================================================

graph has 110 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 1[241]
surviving nonsolo ucounts = 1[241]
ids = [4]

====================================================================================

UMI info for barcode CTGTGCTGTGAGGGTT-1 contig 1 = CCACATCCCT...
umi GCTTAGCGTC = 219 reads: +421 validated

GOOD CONTIGS

TIG 1[bases=486]
0-42 ==> 0-42 on |72|IGHV1-3|5'UTR| [len=42] (mis=0)
42-395 ==> 0-353 on |73|IGHV1-3|L-REGION+V-REGION| [len=353] (mis=12)
415-463 ==> 0-48 on |55|IGHJ4|J-REGION| [len=48] (mis=4)
463-486 ==> 0-23 on |60|IGHM|C-REGION| [len=1358] (mis=0)
junction support: 1 umis using 17 reads
cdr3 = CARGAGFGELWVFDYW at 384, score = 8 + 7
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 216
start codons at 42, 193, 240, 245, 262, 339, 413
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.557 = CTGTGCTTCACAATGC-1

using 20 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.572 = CTGTGCTTCCACTCCA-1

using 271 reads

====================================================================================

graph has 112 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[2^2, 3, 262]
surviving nonsolo ucounts = 1[262]
ids = [3]

====================================================================================

UMI info for barcode CTGTGCTTCCACTCCA-1 contig 1 = GAATCAGTCC...
umi TCCTTAGGCA = 228 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=487]
0-25 ==> 2-27 on |222|IGKV1-17|5'UTR| [len=27] (mis=0)
25-376 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
375-413 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
413-487 ==> 0-74 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 45 reads
cdr3 = CLQYSSSPWTF at 352, score = 9 + 8
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 224
start codons at 25, 31, 100, 236, 455
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.573 = CTGTGCTTCCATTCTA-1

using 103 reads

====================================================================================

graph has 62 edges initially, 2 edges after simplification

total ucounts = 28
nonsolo ucounts = 21[2^5, 3^7, 4, 5^3, 6, 7, 9, 12^2]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.579 = CTGTGCTTCCTACAGA-1

using 524 reads

====================================================================================

graph has 226 edges initially, 2 edges after simplification

total ucounts = 14
nonsolo ucounts = 7[2, 4, 5, 8^2, 229, 261]
surviving nonsolo ucounts = 2[229, 261]
ids = [12, 6]

====================================================================================

UMI info for barcode CTGTGCTTCCTACAGA-1 contig 1 = ACCCAAAAAC...
umi CCCAGGCGGT = 260 reads: +436 validated
umi TGATCCTTTG = 227 reads: +436 validated

GOOD CONTIGS

TIG 1[bases=650]
0-54 ==> 5-59 on |64|IGHV1-18|5'UTR| [len=59] (mis=1)
54-407 ==> 0-353 on |65|IGHV1-18|L-REGION+V-REGION| [len=353] (mis=22)
456-490 ==> 12-46 on |54|IGHJ4|J-REGION| [len=46] (mis=0)
490-650 ==> 0-160 on |1|IGHA1|C-REGION| [len=1058] (mis=0)
junction support: 2 umis using 45 reads
cdr3 = CARDKHFRDPYYHYSGALDHW at 396, score = 7 + 7
umis assigned: [6, 12]
of which 2 are surviving nonsolos
reads assigned: 481
start codons at 54, 252, 257, 274, 318, 351, 544
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.580 = CTGTGCTTCCTATGTT-1

using 214 reads

====================================================================================

graph has 96 edges initially, 4 edges after simplification

total ucounts = 8
nonsolo ucounts = 7[2, 3^3, 5, 50, 147]
surviving nonsolo ucounts = 2[50, 147]
ids = [1, 4]

====================================================================================

UMI info for barcode CTGTGCTTCCTATGTT-1 contig 1 = GGAGGAATCA...
umi TCTAACTAAC = 139 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=437]
29-380 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
379-417 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
417-437 ==> 0-20 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 34 reads
cdr3 = CLQYSSSPWTF at 356, score = 9 + 8
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 134
start codons at 29, 35, 104, 240
confident = false

REJECT CONTIGS

TIG 1[bases=488]
52-119 ==> 6507-6574 on segment before IGHV3OR16-9 exon 1 [len=6692] (mis=2)
83-436 ==> 0-353 on |128|IGHV3-33|L-REGION+V-REGION| [len=353] (mis=18)
cdr3 = CARDTARIA at 425, score = 8 + 4
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 49
start codons at 83, 234, 239, 300, 386
confident = false
not full
not full
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.586 = CTGTGCTTCGAGCCCA-1

using 257 reads

====================================================================================

graph has 108 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 3[2, 6, 246]
surviving nonsolo ucounts = 1[246]
ids = [0]

====================================================================================

UMI info for barcode CTGTGCTTCGAGCCCA-1 contig 1 = AGGAGTCAGA...
umi ACTCATAAAT = 244 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=551]
0-27 ==> 154-181 on |232|IGKV1-5|5'UTR| [len=181] (mis=1)
27-378 ==> 0-351 on |233|IGKV1-5|L-REGION+V-REGION| [len=351] (mis=21)
376-415 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=5)
415-551 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 46 reads
cdr3 = CQQYYTFSHTF at 354, score = 7 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 242
start codons at 27, 33, 89, 102, 185, 238, 241, 334, 457
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.592 = CTGTGCTTCGGGAGTA-1

using 38 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 2[2, 32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.604 = CTGTGCTTCTCCGGTT-1

using 1870 reads

====================================================================================

graph has 2615 edges initially, 36 edges after simplification

total ucounts = 951
nonsolo ucounts = 396[2^182, 3^92, 4^47, 5^30, 6^20, 7^14, 8^6, 10^2, 11, 14, 25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

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BARCODE 52.605 = CTGTGCTTCTCGATGA-1

using 16209 reads

====================================================================================

graph has 11136 edges initially, 92 edges after simplification

total ucounts = 2488
nonsolo ucounts = 1157[2^514, 3^250, 4^148, 5^76, 6^33, 7^29, 8^14, 9^15, 10^9, 11, 12^3, 13^2, 14^2, 15^5, 20, 31, 35, 46, 66, 70, 73, 77, 85, 88, 100, 107, 118, 123, 125, 126, 131, 139, 171, 179, 184, 187, 188, 192, 193^2, 195, 201^2, 203^3, 204^2, 214, 222, 223, 225, 227, 228, 237, 242, 244, 252, 257, 258^2, 261, 263, 284, 288, 331, 364, 417, 428, 829]
surviving nonsolo ucounts = 48[46, 70, 73, 77, 85, 100, 123, 125, 131, 139, 171, 179, 184, 187, 188, 192, 193^2, 195, 201^2, 203^3, 204^2, 214, 222, 223, 225, 227, 228, 237, 242, 244, 252, 257, 258^2, 261, 263, 284, 288, 331, 364, 417, 428, 829]
ids = [15, 508, 2382, 678, 1169, 850, 2169, 2242, 1603, 1965, ...]

====================================================================================

UMI info for barcode CTGTGCTTCTCGATGA-1 contig 1 = GCTGTGCTGT...
umi ACCCACGCAG = 52 reads: -343X +1 -1XX +1 -4XX +2 -3XX +1 -1XX +1 -4XX +1 -3XX +1 -1XX +1 -3XX +1 -4XX +2 invalidated
umi AGTATTACAG = 52 reads: +26 -1X +3 -1X +1 -5X +2 -4XX +239 -42 +27 -1 +27 invalidated
umi ATTTTACCAA = 179 reads: +379 validated
umi CATTACCCGT = 202 reads: +379 validated
umi CCATGTCCAC = 185 reads: +379 validated
umi CGCTTCTCCG = 243 reads: +379 validated
umi CTCAGGCGGT = 179 reads: -343X +1 -1XX +1 -4XX +2 -3XX +1 -1XX +1 -4XX +1 -3XX +1 -1XX +1 -3XX +1 -4XX +2 invalidated
umi GCTTCTAATT = 204 reads: +305 -1XX +73 invalidated
umi GGTAAGGGGT = 37 reads: -343X +1 -1X +1 -4XX +2 -3XX +1 -1XX +1 -4XX +1 -3XX +1 -1XX +1 -3XX +1 -4XX +2 invalidated
umi GTACCGCCAA = 259 reads: +379 validated
umi GTACTTACAC = 223 reads: +379 validated
umi GTCCTCATGA = 197 reads: +379 validated
umi TAACTATATT = 199 reads: +379 validated
umi TATGGATCAC = 206 reads: +379 validated
umi TCACGAGTGG = 240 reads: +379 validated
umi TCATCTCAAT = 227 reads: +379 validated
umi TCATTATGCG = 263 reads: +379 validated
umi TCCCACGAAG = 30 reads: -350X +2 -3XX +1 -1XX +1 -4XX +1 -3XX +1 -1XX +1 -3XX +1 -4XX +2 invalidated
umi TCTCCTATCA = 228 reads: +379 validated
umi TGGTCATTAT = 200 reads: +379 validated
umi TTCGCCGACC = 223 reads: +379 validated

UMI info for barcode CTGTGCTTCTCGATGA-1 contig 2 = TGGGGACCCA...
umi AAAGTATCGA = 41 reads: +411 -13 non-validated
umi ACAATATACA = 195 reads: +424 validated
umi ACGTTACCCA = 367 reads: +424 validated
umi AGTCTCCTTG = 160 reads: +23 -3X +2 -5XX +1 -6XX +1 -1XX +358 -1 +5 -1 +1 -1 +3 -2 +4 -2 +4 invalidated
umi ATGACTCTTT = 293 reads: +424 validated
umi ATGTCTTCTC = 68 reads: +424 validated
umi CAGGGCCAGA = 65 reads: +320 -1 +60 -1 +5 -37 non-validated
umi CCGAGGCGCC = 101 reads: +424 validated
umi CCTATTTACA = 246 reads: +424 validated
umi CCTTATACGT = 134 reads: +29 -4X +1 -6XX +1 -1XX +344 -1 +2 -1 +1 -1 +2 -30 invalidated
umi CGTTCGCCGG = 208 reads: +31 -2X +1 -1 +1 -4X +1 -1XX +354 -1 +2 -1 +7 -2 +15 invalidated
umi CTCATACGCA = 65 reads: +16 -1 +14 -2X +1 -6X +1 -1XX +315 -23 +44 invalidated
umi CTGTTGGGTC = 204 reads: +424 validated
umi GTAGTACAGT = 130 reads: +329 -1 +94 non-validated
umi TCCGAACCCT = 127 reads: +424 validated
umi TCTACAAGGG = 264 reads: -67 +3 -1X +353 invalidated
umi TGATACCGTT = 195 reads: +424 validated
umi TGGACCGGTA = 107 reads: -7 +21 -5XX +1 -6XX +1 -1XX +345 -37 invalidated
umi TGTTAAGTCC = 125 reads: +392 -1 +12 -19 non-validated
umi TGTTTGCCCA = 206 reads: +409 -15 non-validated
umi TTCTCTACGG = 255 reads: +424 validated
umi TTGAATGCAG = 73 reads: +424 validated
umi TTTACTGCCT = 193 reads: +424 validated
umi TTTATCTATA = 146 reads: +37 -1XX +348 -38 invalidated
umi TTTCTTTTGG = 200 reads: +424 validated

GOOD CONTIGS

TIG 1[bases=633]
0-43 ==> 0-43 on |362|IGLV3-25|5'UTR| [len=43] (mis=0)
43-380 ==> 0-337 on |363|IGLV3-25|L-REGION+V-REGION| [len=337] (mis=4)
384-422 ==> 0-38 on |309|IGLJ1|J-REGION| [len=38] (mis=0)
422-633 ==> 0-211 on |305|IGLC1|C-REGION| [len=317] (mis=0)
junction support: 15 umis using 515 reads
cdr3 = CQSVDSSGTYVF at 358, score = 8 + 8
umis assigned: [139, 353, 560, 733, 799, 1002, 1166, 1501, 1557, 1593] and 11 others
of which 20 are surviving nonsolos
reads assigned: 3763
start codons at 43, 104, 173, 191, 386, 554
confident = true

TIG 2[bases=554]
0-59 ==> 0-59 on |64|IGHV1-18|5'UTR| [len=59] (mis=5)
59-412 ==> 0-353 on |65|IGHV1-18|L-REGION+V-REGION| [len=353] (mis=10)
433-483 ==> 0-50 on |53|IGHJ3|J-REGION| [len=50] (mis=1)
483-554 ==> 0-71 on |60|IGHM|C-REGION| [len=1358] (mis=0)
junction support: 18 umis using 306 reads
cdr3 = CARNFDIVAYGDAFDIW at 401, score = 8 + 8
umis assigned: [15, 86, 199, 359, 480, 508, 678, 850, 889, 926] and 15 others
of which 25 are surviving nonsolos
reads assigned: 4110
start codons at 59, 182, 210, 257, 294, 323, 356, 429, 435, 464
confident = true

REJECT CONTIGS

TIG 1[bases=304]
5-100 ==> 2550-2645 on rc of segment before IGHD1-1 exon 1 [len=2645] (mis=0)
139-202 ==> 0-63 on |59|IGHJ6|J-REGION| [len=63] (mis=1)
202-304 ==> 0-102 on |6|IGHD|C-REGION| [len=1289] (mis=0)
umis assigned: [444, 996]
of which 2 are surviving nonsolos
reads assigned: 1190
start codons at 159, 220, 281
confident = false
did not find CDR3
now this is a cell
paired!

GACACGGCCGTGTATTACTGTGCGAGAAATTTTGATATAGTGGCTTATGGAGATGCTTTTGATATCTGGGGCCAAGGGACAATGGTCACCGTCTCTTCAG <==> AGTGGAGTCCAGGCAGAAGACGAGGCTGACTATTACTGTCAATCAGTAGACAGCAGTGGTACTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAG

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BARCODE 52.609 = CTGTGCTTCTCTGCTG-1

using 17 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[3, 4, 8]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

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BARCODE 52.625 = CTGTGCTTCTTTAGGG-1

using 2306 reads

====================================================================================

graph has 3400 edges initially, 18 edges after simplification

total ucounts = 1080
nonsolo ucounts = 512[2^217, 3^121, 4^79, 5^43, 6^13, 7^17, 8^12, 9^3, 10^2, 11, 12, 13^2, 21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!
sorting bam, mem = 0.05
running samtools sort -l 8G -o /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk052-ucc6a7a72ce/files/contig_bam_sorted.bam /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk052-ucc6a7a72ce/files/contig_bam.bam

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15.256 seconds used processing barcodes, peak mem = 0.23
