[stdout]

starting vdj_asm_asm, mem = 0.01
available mem = 844.34 GB
/scratch/etanis/software/cellranger-6.1.2/lib/bin/cr_vdj martian assembly main /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GE...
n50_n50_rpu = 288

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.0 = CAACTAGTCTCGTATT-1

using 73784 reads

====================================================================================

graph has 19809 edges initially, 114 edges after simplification

total ucounts = 3024
nonsolo ucounts = 1598[2^527, 3^300, 4^173, 5^121, 6^75, 7^40, 8^29, 9^16, 10^6, 11^7, 12^5, 13^3, 14^3, 15, 16^2, 18, 19^2, 20, 21, 25, 27, 28^2, 29, 32, 3...
surviving nonsolo ucounts = 258[84, 97, 102, 105^2, 109, 111^2, 112, 114, 115, 116^2, 117, 118^2, 119, 120, 121^2, 124, 128^2, 130, 133^3, 135^2, 137, 138, ...
ids = [2292, 222, 0, 1473, 1581, 1467, 1669, 1703, 1296, 3002, ...]

====================================================================================

UMI info for barcode CAACTAGTCTCGTATT-1 contig 1 = TGAGCGCAGA...
umi AAAAACTCCA = 102 reads: +388 validated
umi AAAAGGTTTC = 156 reads: +388 validated
umi AAAATAGCCT = 244 reads: +388 validated
umi AAACGCACCT = 228 reads: +388 validated
umi AAAGACGTGC = 270 reads: +388 validated
umi AAATAGGTCA = 262 reads: +388 validated
umi AACCAACCCG = 309 reads: +388 validated
umi AACGTTGCAG = 209 reads: +388 validated
umi AATATGCCTA = 113 reads: +31 -2X +2 -1XX +1 -7XX +206 -1 +1 -1 +135 invalidated
umi AATCCGGCAT = 221 reads: +388 validated
umi AATCTTCCTT = 229 reads: +388 validated
umi AATGTGGGGT = 166 reads: +388 validated
umi AATTTTCTGT = 216 reads: +388 validated
umi ACACTAATCC = 243 reads: +388 validated
umi ACAGTTGTCC = 286 reads: +388 validated
umi ACATACACGT = 222 reads: +388 validated
umi ACATGACCTT = 266 reads: +388 validated
umi ACATGTATAG = 212 reads: +388 validated
umi ACCAATCTTG = 99 reads: +388 validated
umi ACCACAAGCG = 228 reads: +388 validated
umi ACCCATGGCT = 268 reads: +388 validated
umi ACCGACCGGG = 286 reads: +388 validated
umi ACCTTTCAGA = 94 reads: -10 +1 -1XX +1 -2XX +2 -1XX +11 -1XX +3 -2XX +3 -2XX +24 -3XX +4 -1XX +5 -2XX +4 -1XX +7 -1XX +1 -1XX +1 -1XX +2 -1XX +6 -2XX +1 ...
umi ACGTTTGACT = 207 reads: +388 validated
umi ACTAACCGGG = 241 reads: +388 validated
umi ACTAGTCCCG = 190 reads: +37 -7XX +344 invalidated
umi ACTCGCTTAC = 170 reads: +388 validated
umi ACTGTCTCAG = 215 reads: +388 validated
umi ACTTCTACGC = 203 reads: +388 validated
umi ACTTTAGAAG = 287 reads: +388 validated
umi AGAGCTGCGG = 157 reads: +388 validated
umi AGATGTAGTC = 223 reads: +388 validated
umi AGCAGACAAA = 496 reads: -331X +1 -2XX +2 -14XX +1 -1XX +2 -1XX +1 -1XX +8 -1XX +22 invalidated
umi AGCCGACCGC = 246 reads: +388 validated
umi AGCGCGCGCT = 157 reads: +388 validated
umi AGCTCACGAT = 254 reads: +388 validated
umi AGGTATATTC = 272 reads: +388 validated
umi AGGTCTGCAA = 217 reads: +388 validated
umi AGTGGTCCCA = 102 reads: +41 -3X +292 -52 invalidated
umi AGTTTGGCTA = 259 reads: +388 validated
umi ATAAATTATT = 129 reads: +388 validated
umi ATACGTCTGT = 106 reads: +42 -2XX +278 -1 +4 -1 +1 -4 +55 invalidated
umi ATACGTTCAT = 122 reads: +388 validated
umi ATATCAATCG = 838 reads: -331 +57 non-validated
umi ATATTGCTCA = 205 reads: +388 validated
umi ATCAAAACCA = 199 reads: +388 validated
umi ATCCGTTTGG = 415 reads: +388 validated
umi ATGATCGTGC = 253 reads: +388 validated
umi ATGCGTTTCA = 223 reads: +388 validated
umi ATTAATAGCG = 117 reads: +388 validated
umi ATTATTGCAT = 89 reads: +47 -2XX +2 -1X +308 -5 +23 invalidated
umi ATTCCCCCTC = 219 reads: +388 validated
umi ATTCTTGGTT = 231 reads: +388 validated
umi ATTTTATCTG = 244 reads: +388 validated
umi ATTTTGCGGG = 139 reads: +48 -1 +339 non-validated
umi CAACAGTAGC = 660 reads: -368 +1 -2XX +17 invalidated
umi CAACGACCAA = 134 reads: +388 validated
umi CAAGTTCCTT = 651 reads: -350X +1 -1XX +2 -1XX +1 -1XX +8 -1XX +22 invalidated
umi CAATAGTCAC = 236 reads: +388 validated
umi CAATATAAGG = 258 reads: -331X +1 -2X +2 -14XX +1 -1XX +2 -1XX +1 -1XX +8 -1XX +22 invalidated
umi CAATCAATCA = 259 reads: +388 validated
umi CACAGGCGAA = 277 reads: +388 validated
umi CACGTTGCTG = 324 reads: +388 validated
umi CACTCGAAGT = 209 reads: +388 validated
umi CAGACATTGT = 290 reads: +388 validated
umi CAGCGTCACC = 202 reads: +388 validated
umi CAGTGTCTAG = 301 reads: +388 validated
umi CATATCTATT = 245 reads: +33 -1XX +354 invalidated
umi CATATTAGTC = 258 reads: +33 -1XX +354 invalidated
umi CCAAAGGGGT = 205 reads: +388 validated
umi CCACACGCCG = 184 reads: +388 validated
umi CCACGGCGAC = 517 reads: +388 validated
umi CCAGGTTCTT = 801 reads: -388 non-validated
umi CCATGCCTCT = 222 reads: +388 validated
umi CCATTAGACG = 280 reads: +388 validated
... 24065 lines elided ...

BARCODE 16.1568 = CACAGTAAGGACAGCT-1

using 369 reads

====================================================================================

graph has 172 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 1[367]
surviving nonsolo ucounts = 1[367]
ids = [1]

====================================================================================

UMI info for barcode CACAGTAAGGACAGCT-1 contig 1 = GGGGAGGAAC...
umi AGAGAAATAA = 352 reads: +385 validated

GOOD CONTIGS

TIG 1[bases=515]
0-36 ==> 11-47 on |278|IGKV3-11|5'UTR| [len=47] (mis=0)
36-381 ==> 0-345 on |279|IGKV3-11|L-REGION+V-REGION| [len=345] (mis=0)
384-421 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=0)
421-515 ==> 0-94 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 64 reads
cdr3 = CQQRSNWPPLTF at 357, score = 9 + 9
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 343
start codons at 36, 241, 244, 463
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1578 = CACAGTAAGTTTAGGA-1

using 185 reads

====================================================================================

graph has 98 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[2, 181]
surviving nonsolo ucounts = 1[181]
ids = [2]

====================================================================================

UMI info for barcode CACAGTAAGTTTAGGA-1 contig 1 = CAAAAACCAC...
umi TCTATATCTG = 170 reads: +436 validated

GOOD CONTIGS

TIG 1[bases=492]
0-51 ==> 8-59 on |64|IGHV1-18|5'UTR| [len=59] (mis=1)
51-404 ==> 0-353 on |65|IGHV1-18|L-REGION+V-REGION| [len=353] (mis=22)
453-487 ==> 12-46 on |54|IGHJ4|J-REGION| [len=46] (mis=0)
junction support: 1 umis using 19 reads
cdr3 = CARDKHFRDPYYHYSGALDHW at 393, score = 7 + 7
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 169
start codons at 51, 249, 254, 271, 315, 348
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1582 = CACAGTACAAATCCGT-1

using 523 reads

====================================================================================

graph has 254 edges initially, 12 edges after simplification

total ucounts = 6
nonsolo ucounts = 5[2, 5, 6, 178, 331]
surviving nonsolo ucounts = 2[178, 331]
ids = [0, 4]

====================================================================================

UMI info for barcode CACAGTACAAATCCGT-1 contig 1 = CAGTCAGGAC...
umi ATTTACATTC = 181 reads: +391 validated

UMI info for barcode CACAGTACAAATCCGT-1 contig 2 = GATCAGGACT...
umi GGGGTTCTCG = 332 reads: +400 validated

GOOD CONTIGS

TIG 1[bases=542]
15-368 ==> 0-353 on |253|IGKV1D-39|L-REGION+V-REGION| [len=353] (mis=0)
368-406 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=0)
406-542 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 25 reads
cdr3 = CQQSYSTPPWTF at 342, score = 9 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 174
start codons at 15, 21, 77, 90, 226, 448
confident = false

TIG 2[bases=566]
0-30 ==> 0-30 on |264|IGKV2-28|5'UTR| [len=30] (mis=0)
30-390 ==> 0-360 on |265|IGKV2-28|L-REGION+V-REGION| [len=360] (mis=1)
392-430 ==> 0-38 on |215|IGKJ3|J-REGION| [len=38] (mis=0)
430-566 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 55 reads
cdr3 = CMQALQTPLFTF at 366, score = 9 + 8
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 325
start codons at 30, 63, 99, 187, 349, 369, 472
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1591 = CACAGTACAATAGCAA-1

using 332 reads

====================================================================================

graph has 138 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 5[3, 4, 7, 8, 306]
surviving nonsolo ucounts = 1[306]
ids = [6]

====================================================================================

UMI info for barcode CACAGTACAATAGCAA-1 contig 1 = GAGTCAGACT...
umi TCTTCAGCTT = 293 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=507]
0-25 ==> 156-181 on |232|IGKV1-5|5'UTR| [len=181] (mis=1)
25-376 ==> 0-351 on |233|IGKV1-5|L-REGION+V-REGION| [len=351] (mis=27)
375-413 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=6)
413-507 ==> 0-94 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 49 reads
cdr3 = CQQYNGQSRAF at 352, score = 8 + 7
umis assigned: [6]
of which 1 are surviving nonsolos
reads assigned: 286
start codons at 25, 31, 100, 236, 239, 332, 365, 455
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1601 = CACAGTACAGACGTAG-1

using 302 reads

====================================================================================

graph has 172 edges initially, 2 edges after simplification

total ucounts = 15
nonsolo ucounts = 8[2^4, 3^2, 6, 275]
surviving nonsolo ucounts = 1[275]
ids = [14]

====================================================================================

UMI info for barcode CACAGTACAGACGTAG-1 contig 1 = AGCTGTGGGC...
umi TGCAATCCTC = 261 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=542]
0-40 ==> 0-40 on |356|IGLV3-19|5'UTR| [len=40] (mis=0)
40-377 ==> 0-337 on |357|IGLV3-19|L-REGION+V-REGION| [len=337] (mis=11)
384-422 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=2)
422-542 ==> 0-120 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 43 reads
cdr3 = CNSRDSSGNYVIF at 355, score = 8 + 9
umis assigned: [14]
of which 1 are surviving nonsolos
reads assigned: 258
start codons at 40, 159, 188, 239, 338, 383
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1603 = CACAGTACAGAGCCAA-1

using 23 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[3, 4, 5, 9]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1606 = CACAGTACAGCTGTGC-1

using 15 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1610 = CACAGTACAGTCAGAG-1

using 1761 reads

====================================================================================

graph has 2476 edges initially, 10 edges after simplification

total ucounts = 684
nonsolo ucounts = 326[2^108, 3^89, 4^43, 5^36, 6^17, 7^9, 8^8, 9^5, 10^3, 11^2, 12^2, 13, 14, 20, 171]
surviving nonsolo ucounts = 1[171]
ids = [462]

====================================================================================

UMI info for barcode CACAGTACAGTCAGAG-1 contig 1 = GGGGTCTCAG...
umi TAAGTATGCT = 169 reads: +391 validated

GOOD CONTIGS

TIG 1[bases=502]
0-38 ==> 125-163 on |346|IGLV2-8|5'UTR| [len=163] (mis=0)
38-392 ==> 0-354 on |347|IGLV2-8|L-REGION+V-REGION| [len=354] (mis=2)
391-429 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=2)
429-502 ==> 0-73 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 33 reads
cdr3 = CSSYAGTNNFGVF at 362, score = 8 + 8
umis assigned: [462]
of which 1 are surviving nonsolos
reads assigned: 166
start codons at 38, 195, 239, 246, 345, 372
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1615 = CACAGTACATCCCATC-1

using 310 reads

====================================================================================

graph has 86 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 2[2, 304]
surviving nonsolo ucounts = 1[304]
ids = [5]

====================================================================================

UMI info for barcode CACAGTACATCCCATC-1 contig 1 = GGAGGAATCA...
umi TGTCACCGTA = 285 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=503]
29-380 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
379-417 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
417-503 ==> 0-86 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 42 reads
cdr3 = CLQYSSSPWTF at 356, score = 9 + 8
umis assigned: [5]
of which 1 are surviving nonsolos
reads assigned: 282
start codons at 29, 35, 104, 240, 459
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1616 = CACAGTACATCCTAGA-1

using 447 reads

====================================================================================

graph has 194 edges initially, 4 edges after simplification

total ucounts = 10
nonsolo ucounts = 7[2, 3, 4^2, 5, 94, 332]
surviving nonsolo ucounts = 1[332]
ids = [3]

====================================================================================

UMI info for barcode CACAGTACATCCTAGA-1 contig 1 = GGAGTGCTTT...
umi CCCATGACCA = 325 reads: +436 validated

GOOD CONTIGS

TIG 1[bases=574]
19-390 ==> 0-371 on |186|IGHV4-34|L-REGION+V-REGION| [len=371] (mis=8)
407-455 ==> 0-48 on |55|IGHJ4|J-REGION| [len=48] (mis=5)
455-574 ==> 0-119 on |45|IGHG3|C-REGION| [len=1130] (mis=1)
junction support: 1 umis using 34 reads
cdr3 = CARAHGDYYTLLDFW at 379, score = 8 + 7
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 320
start codons at 19, 40, 84, 170, 370
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1621 = CACAGTACATTAACCG-1

using 266 reads

====================================================================================

graph has 116 edges initially, 2 edges after simplification

total ucounts = 14
nonsolo ucounts = 6[2, 3^2, 4, 5, 241]
surviving nonsolo ucounts = 1[241]
ids = [2]

====================================================================================

UMI info for barcode CACAGTACATTAACCG-1 contig 1 = TGGAATCAGA...
umi ACACCCCATC = 225 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=429]
0-27 ==> 0-27 on |245|IGKV1D-16|5'UTR| [len=27] (mis=1)
27-378 ==> 0-351 on |246|IGKV1D-16|L-REGION+V-REGION| [len=351] (mis=0)
378-415 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=0)
junction support: 1 umis using 32 reads
cdr3 = CQQYNSYPLTF at 354, score = 9 + 9
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 223
start codons at 27, 33, 89, 102, 238
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1622 = CACAGTACATTAGGCT-1

using 221 reads

====================================================================================

graph has 98 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[221]
surviving nonsolo ucounts = 1[221]
ids = [0]

====================================================================================

UMI info for barcode CACAGTACATTAGGCT-1 contig 1 = GGGGTCTCAG...
umi GCTACCACCG = 214 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=516]
0-38 ==> 3-41 on |338|IGLV2-14|5'UTR| [len=41] (mis=3)
38-382 ==> 0-344 on |339|IGLV2-14|L-REGION+V-REGION| [len=361] (mis=20)
388-426 ==> 0-38 on |309|IGLJ1|J-REGION| [len=38] (mis=4)
426-516 ==> 0-90 on |305|IGLC1|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 30 reads
cdr3 = CSSYISSTTLGF at 362, score = 8 + 9
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 210
start codons at 38, 246, 249
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 16.1623 = CACAGTACATTCACTT-1

using 23 reads

====================================================================================

graph has 6 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

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BARCODE 16.1641 = CACAGTAGTCTAAAGA-1

using 313 reads

====================================================================================

graph has 130 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[2^2, 307]
surviving nonsolo ucounts = 1[307]
ids = [1]

====================================================================================

UMI info for barcode CACAGTAGTCTAAAGA-1 contig 1 = TGGGAGGAAT...
umi CTAGTCCGCT = 310 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=555]
31-382 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
381-419 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
419-555 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 40 reads
cdr3 = CLQYSSSPWTF at 358, score = 9 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 303
start codons at 31, 37, 106, 242, 461
confident = false
NOT paired!

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BARCODE 16.1644 = CACAGTAGTCTGGAGA-1

using 161 reads

====================================================================================

graph has 62 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 2[2, 152]
surviving nonsolo ucounts = 1[152]
ids = [3]

====================================================================================

UMI info for barcode CACAGTAGTCTGGAGA-1 contig 1 = GGGGTCTCAG...
umi AGGCACTTCA = 150 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=482]
0-38 ==> 125-163 on |346|IGLV2-8|5'UTR| [len=163] (mis=0)
38-392 ==> 0-354 on |347|IGLV2-8|L-REGION+V-REGION| [len=354] (mis=13)
388-426 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=5)
426-482 ==> 0-56 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 25 reads
cdr3 = CSSHAGSTRVLF at 362, score = 8 + 8
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 148
start codons at 38, 195, 246, 345, 372
confident = false
NOT paired!
sorting bam, mem = 0.15
running samtools sort -l 8G -o /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ...

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162.014 seconds used processing barcodes, peak mem = 0.34
