[stdout]

starting vdj_asm_asm, mem = 0.01
available mem = 844.38 GB
/scratch/etanis/software/cellranger-6.1.2/lib/bin/cr_vdj martian assembly main /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk003-ucc6a7a72ce /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk003-ucc6a7a72ce/files /scratch/etanis/ST-118/multi/dimitri-multi/journal/SC_MULTI_CS.SC_MULTI_CORE.MULTI_GEM_WELL_PROCESSOR.VDJ_B_GEM_WELL_PROCESSOR.SC_VDJ_CONTIG_ASSEMBLER.ASSEMBLE_VDJ.fork0.chnk003.ucc6a7a72ce
n50_n50_rpu = 288

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 3.0 = AACTTTCGTATCTGCA-1

using 632 reads

====================================================================================

graph has 160 edges initially, 2 edges after simplification

total ucounts = 15
nonsolo ucounts = 6[2^2, 3^3, 610]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 3.2 = AACTTTCGTCAAAGAT-1

using 417 reads

====================================================================================

graph has 182 edges initially, 4 edges after simplification

total ucounts = 6
nonsolo ucounts = 3[2, 169, 243]
surviving nonsolo ucounts = 2[169, 243]
ids = [1, 5]

====================================================================================

UMI info for barcode AACTTTCGTCAAAGAT-1 contig 1 = ATCAGACCCA...
umi CTAATAGGTA = 164 reads: +388 validated

UMI info for barcode AACTTTCGTCAAAGAT-1 contig 2 = GCTCTGCTTC...
umi TTGTGCTTAA = 238 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=471]
0-23 ==> 4-27 on |220|IGKV1-16|5'UTR| [len=27] (mis=0)
23-374 ==> 0-351 on |221|IGKV1-16|L-REGION+V-REGION| [len=351] (mis=6)
379-411 ==> 7-39 on |214|IGKJ2|J-REGION| [len=39] (mis=1)
411-471 ==> 0-60 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 24 reads
cdr3 = CQQYNSYPPTF at 350, score = 9 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 160
start codons at 23, 29, 85, 98, 234, 453
confident = false

TIG 2[bases=567]
0-51 ==> 0-51 on |320|IGLV1-40|5'UTR| [len=51] (mis=0)
51-407 ==> 0-356 on |321|IGLV1-40|L-REGION+V-REGION| [len=356] (mis=12)
407-445 ==> 0-38 on |309|IGLJ1|J-REGION| [len=38] (mis=1)
445-567 ==> 0-122 on |305|IGLC1|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 32 reads
cdr3 = CQSYDSSLSGLYVF at 375, score = 7 + 8
umis assigned: [5]
of which 1 are surviving nonsolos
reads assigned: 236
start codons at 51, 205, 208, 259, 358, 385, 409
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 3.5 = AACTTTCGTCATATCG-1

using 401 reads

====================================================================================

graph has 166 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[5^2, 390]
surviving nonsolo ucounts = 1[390]
ids = [2]

====================================================================================

UMI info for barcode AACTTTCGTCATATCG-1 contig 1 = GATCAGGACT...
umi GCGAATCGTC = 378 reads: +397 validated

GOOD CONTIGS

TIG 1[bases=523]
0-30 ==> 0-30 on |264|IGKV2-28|5'UTR| [len=30] (mis=0)
30-390 ==> 0-360 on |265|IGKV2-28|L-REGION+V-REGION| [len=360] (mis=0)
389-427 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=2)
427-523 ==> 0-96 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 58 reads
cdr3 = CMQALQTPRTF at 366, score = 9 + 8
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 371
start codons at 30, 63, 99, 187, 349, 369, 469
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 3.6 = AACTTTCGTCATGCAT-1

using 29 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 1[27]
surviving nonsolo ucounts = 1[27]
ids = [2]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 3.8 = AACTTTCGTCCTGCTT-1

using 1271 reads

====================================================================================

graph has 1946 edges initially, 4 edges after simplification

total ucounts = 633
nonsolo ucounts = 265[2^113, 3^75, 4^24, 5^21, 6^15, 7^5, 8^3, 9^4, 10, 12, 14, 15^2]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 3.12 = AACTTTCGTGACTACT-1

using 236 reads

====================================================================================

graph has 68 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 2[4, 228]
surviving nonsolo ucounts = 1[228]
ids = [0]

====================================================================================

UMI info for barcode AACTTTCGTGACTACT-1 contig 1 = GCTCTGCTTC...
umi AATTAACGGT = 226 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=543]
0-51 ==> 0-51 on |320|IGLV1-40|5'UTR| [len=51] (mis=0)
51-407 ==> 0-356 on |321|IGLV1-40|L-REGION+V-REGION| [len=356] (mis=12)
407-445 ==> 0-38 on |309|IGLJ1|J-REGION| [len=38] (mis=1)
445-543 ==> 0-98 on |305|IGLC1|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 33 reads
cdr3 = CQSYDSSLSGLYVF at 375, score = 7 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 224
start codons at 51, 205, 208, 259, 358, 385, 409
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 3.15 = AACTTTCGTGGTCTCG-1

using 303 reads

====================================================================================

graph has 154 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 2[8, 291]
surviving nonsolo ucounts = 1[291]
ids = [3]

====================================================================================

UMI info for barcode AACTTTCGTGGTCTCG-1 contig 1 = GTCAGACTCA...
umi TCAGTACCCT = 286 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=454]
0-23 ==> 158-181 on |232|IGKV1-5|5'UTR| [len=181] (mis=1)
23-374 ==> 0-351 on |233|IGKV1-5|L-REGION+V-REGION| [len=351] (mis=6)
373-411 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
411-454 ==> 0-43 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 36 reads
cdr3 = CQQYNSYSRTF at 350, score = 8 + 8
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 275
start codons at 23, 29, 85, 98, 234, 237, 330
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 3.16 = AACTTTCGTGTCTGAT-1

using 139 reads

====================================================================================

graph has 54 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[3, 7, 128]
surviving nonsolo ucounts = 1[128]
ids = [0]

====================================================================================

UMI info for barcode AACTTTCGTGTCTGAT-1 contig 1 = GATCAGGACT...
umi AGTTAGGAAT = 121 reads: +397 validated

GOOD CONTIGS

TIG 1[bases=455]
0-30 ==> 0-30 on |264|IGKV2-28|5'UTR| [len=30] (mis=0)
30-356 ==> 0-326 on |265|IGKV2-28|L-REGION+V-REGION| [len=360] (mis=11)
389-427 ==> 0-38 on |215|IGKJ3|J-REGION| [len=38] (mis=2)
427-455 ==> 0-28 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 16 reads
cdr3 = CMNTLPGGFTF at 366, score = 8 + 7
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 121
start codons at 30, 63, 99, 187, 349, 369
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 3.18 = AACTTTCGTGTTCGAT-1

using 155 reads

====================================================================================

graph has 188 edges initially, 4 edges after simplification

total ucounts = 32
nonsolo ucounts = 26[2^6, 3^6, 5, 6^4, 7, 8, 9^2, 10, 11^2, 12, 13]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 3.19 = AACTTTCGTTAAGATG-1

using 202 reads

====================================================================================

graph has 104 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 3[3, 6, 189]
surviving nonsolo ucounts = 1[189]
ids = [3]

====================================================================================

UMI info for barcode AACTTTCGTTAAGATG-1 contig 1 = GTCCTCTAGA...
umi AGGTAGATCC = 188 reads: +409 validated

GOOD CONTIGS

TIG 1[bases=518]
0-38 ==> 26-64 on |66|IGHV1-2|5'UTR| [len=64] (mis=0)
38-391 ==> 0-353 on |67|IGHV1-2|L-REGION+V-REGION| [len=353] (mis=2)
401-447 ==> 0-46 on |54|IGHJ4|J-REGION| [len=46] (mis=5)
447-518 ==> 0-71 on |60|IGHM|C-REGION| [len=1358] (mis=0)
junction support: 1 umis using 11 reads
cdr3 = CARGKMVLPDYW at 380, score = 8 + 7
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 184
start codons at 38, 194, 236, 241, 273, 302, 335, 395
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 3.23 = AACTTTCGTTCGTGAT-1

using 33 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 3.33 = AACTTTCTCACATGCA-1

using 14 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[14]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 3.36 = AACTTTCTCACTTATC-1

using 39 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 2[2, 33]
surviving nonsolo ucounts = 1[33]
ids = [3]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 3.39 = AACTTTCTCATGTAGC-1

using 662 reads

====================================================================================

graph has 150 edges initially, 4 edges after simplification

total ucounts = 6
nonsolo ucounts = 2[118, 540]
surviving nonsolo ucounts = 2[118, 540]
ids = [0, 4]

====================================================================================

UMI info for barcode AACTTTCTCATGTAGC-1 contig 1 = CTCAGGACAC...
umi CCACGCAGCC = 117 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=471]
13-364 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
363-401 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
401-471 ==> 0-70 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 26 reads
cdr3 = CLQYSSSPWTF at 340, score = 9 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 116
start codons at 13, 19, 88, 224, 443
confident = false

REJECT CONTIGS

TIG 1[bases=467]
4-218 ==> 142-356 on |321|IGLV1-40|L-REGION+V-REGION| [len=356] (mis=8)
218-256 ==> 0-38 on |309|IGLJ1|J-REGION| [len=38] (mis=1)
256-467 ==> 0-211 on |305|IGLC1|C-REGION| [len=317] (mis=0)
cdr3 = CQSYDSSLSGLYVF at 186, score = 7 + 8
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 533
start codons at 16, 19, 70, 169, 196, 220, 388
confident = false
not full
VJ delta = 22
not full
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 3.43 = AACTTTCTCCCTAATT-1

using 443 reads

====================================================================================

graph has 162 edges initially, 6 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[207, 235]
surviving nonsolo ucounts = 2[207, 235]
ids = [2, 0]

====================================================================================

UMI info for barcode AACTTTCTCCCTAATT-1 contig 1 = AGCTTCAGCT...
umi TCGGGTCTTT = 207 reads: +388 validated

UMI info for barcode AACTTTCTCCCTAATT-1 contig 2 = GTGGGCTCAG...
umi CCAACGCTTC = 238 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=594]
0-47 ==> 67-114 on |322|IGLV1-44|5'UTR| [len=114] (mis=0)
47-400 ==> 0-353 on |323|IGLV1-44|L-REGION+V-REGION| [len=353] (mis=15)
397-435 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=3)
435-594 ==> 0-159 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 35 reads
cdr3 = CASWDDSLRGRVF at 368, score = 8 + 8
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 203
start codons at 47, 201, 351, 376, 381
confident = false

TIG 2[bases=576]
0-35 ==> 0-35 on |350|IGLV3-10|5'UTR| [len=35] (mis=0)
35-382 ==> 0-347 on |351|IGLV3-10|L-REGION+V-REGION| [len=347] (mis=10)
379-417 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=1)
417-576 ==> 0-159 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 44 reads
cdr3 = CYSTDSSGNRRVF at 350, score = 7 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 229
start codons at 35, 96, 165, 183, 234, 296, 333
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 3.45 = AACTTTCTCCTCCTAG-1

using 127 reads

====================================================================================

graph has 58 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 4[2^2, 3, 115]
surviving nonsolo ucounts = 1[115]
ids = [5]

====================================================================================

UMI info for barcode AACTTTCTCCTCCTAG-1 contig 1 = AGTCTGGGCC...
umi GTAGCTGCAG = 108 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=458]
0-40 ==> 211-251 on |358|IGLV3-21|5'UTR| [len=251] (mis=0)
40-385 ==> 0-345 on |359|IGLV3-21|L-REGION+V-REGION| [len=351] (mis=30)
384-422 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=1)
422-458 ==> 0-36 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 10 reads
cdr3 = CQVWYSNSDHVVF at 355, score = 7 + 8
umis assigned: [5]
of which 1 are surviving nonsolos
reads assigned: 108
start codons at 40, 235, 242, 338, 383
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 3.49 = AACTTTCTCGCCAAAT-1

using 134 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 1[130]
surviving nonsolo ucounts = 1[130]
ids = [3]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 3.50 = AACTTTCTCGCCATAA-1

using 299 reads

====================================================================================

graph has 88 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 1[293]
surviving nonsolo ucounts = 1[293]
ids = [1]

====================================================================================

UMI info for barcode AACTTTCTCGCCATAA-1 contig 1 = GAGGAGTCAG...
umi CATGTGGTAT = 276 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=499]
28-381 ==> 0-353 on |253|IGKV1D-39|L-REGION+V-REGION| [len=353] (mis=11)
379-416 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=0)
416-499 ==> 0-83 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 38 reads
cdr3 = CQQSYTTLLTF at 355, score = 9 + 9
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 273
start codons at 28, 34, 90, 103, 458
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 3.53 = AACTTTCTCGGCGCAT-1

using 298 reads

====================================================================================

graph has 138 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 4[2, 5, 8, 280]
surviving nonsolo ucounts = 1[280]
ids = [3]

====================================================================================

UMI info for barcode AACTTTCTCGGCGCAT-1 contig 1 = GAATCAGTCC...
umi CGCTGACTCC = 270 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=508]
0-25 ==> 2-27 on |222|IGKV1-17|5'UTR| [len=27] (mis=0)
25-376 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
375-413 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
413-508 ==> 0-95 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 48 reads
cdr3 = CLQYSSSPWTF at 352, score = 9 + 8
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 265
start codons at 25, 31, 100, 236, 455
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 3.56 = AACTTTCTCGTACCGG-1

using 44 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 3[2, 3, 36]
surviving nonsolo ucounts = 1[36]
ids = [3]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 3.59 = AACTTTCTCTACCTGC-1

using 69 reads

====================================================================================

graph has 66 edges initially, 4 edges after simplification

total ucounts = 10
nonsolo ucounts = 6[2, 3, 5, 6, 18, 31]
surviving nonsolo ucounts = 2[5, 31]
ids = [3, 7]

====================================================================================

REJECT CONTIGS

TIG 1[bases=327]
0-260 ==> 77-337 on |363|IGLV3-25|L-REGION+V-REGION| [len=337] (mis=0)
267-305 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=0)
305-327 ==> 0-22 on |306|IGLC2|C-REGION| [len=317] (mis=0)
cdr3 = CQSADSSGTYVVF at 238, score = 8 + 8
umis assigned: [3, 7]
of which 2 are surviving nonsolos
reads assigned: 24
start codons at 53, 71, 266
confident = false
not full
VJ delta = 12
not full
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 3.61 = AACTTTCTCTATCGCC-1

using 88 reads

====================================================================================

graph has 96 edges initially, 4 edges after simplification

total ucounts = 15
nonsolo ucounts = 11[2, 4^3, 7^2, 9, 11^2, 12, 13]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 3.63 = AACTTTCTCTCCAGGG-1

using 231 reads

====================================================================================

graph has 86 edges initially, 2 edges after simplification

total ucounts = 13
nonsolo ucounts = 8[2^3, 3, 4^2, 10, 199]
surviving nonsolo ucounts = 1[199]
ids = [0]

====================================================================================

UMI info for barcode AACTTTCTCTCCAGGG-1 contig 1 = ACTTTCTGAG...
umi AATGCTTCCA = 197 reads: +415 validated

GOOD CONTIGS

TIG 1[bases=517]
0-35 ==> 110-145 on |193|IGHV4-59|5'UTR| [len=145] (mis=0)
35-385 ==> 0-350 on |194|IGHV4-59|L-REGION+V-REGION| [len=350] (mis=17)
400-450 ==> 0-50 on |53|IGHJ3|J-REGION| [len=50] (mis=4)
450-517 ==> 0-67 on |40|IGHG1|C-REGION| [len=884] (mis=0)
junction support: 1 umis using 23 reads
cdr3 = CARDFPGNYFTFDIW at 374, score = 9 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 194
start codons at 35, 79, 159, 229, 431
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 3.66 = AACTTTCTCTGTCAAG-1

using 252 reads

====================================================================================

graph has 112 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 5[2, 4^2, 5, 233]
surviving nonsolo ucounts = 1[233]
ids = [0]

====================================================================================

UMI info for barcode AACTTTCTCTGTCAAG-1 contig 1 = AGGAGGCAGC...
umi AATACAACGT = 229 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=517]
30-391 ==> 0-361 on |339|IGLV2-14|L-REGION+V-REGION| [len=361] (mis=11)
380-418 ==> 0-38 on |309|IGLJ1|J-REGION| [len=38] (mis=3)
418-517 ==> 0-99 on |305|IGLC1|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 39 reads
cdr3 = CSSYTSSSTLVF at 354, score = 8 + 9
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 225
start codons at 30, 187, 231, 238, 241
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 3.74 = AAGACCTAGACTAGGC-1

using 150 reads

====================================================================================

graph has 54 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 4[2, 3, 10, 132]
surviving nonsolo ucounts = 1[132]
ids = [6]

====================================================================================

UMI info for barcode AAGACCTAGACTAGGC-1 contig 1 = TGGGAGTCAG...
umi TTTCTGTACG = 127 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=478]
28-381 ==> 0-353 on |253|IGKV1D-39|L-REGION+V-REGION| [len=353] (mis=29)
379-416 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=1)
416-478 ==> 0-62 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 32 reads
cdr3 = CQQGYSIPLTF at 355, score = 9 + 9
umis assigned: [6]
of which 1 are surviving nonsolos
reads assigned: 126
start codons at 28, 34, 90, 239, 293, 458
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 3.76 = AAGACCTAGATGCCAG-1

using 12432 reads

====================================================================================

graph has 9335 edges initially, 162 edges after simplification

total ucounts = 2399
nonsolo ucounts = 1804[2^326, 3^274, 4^223, 5^192, 6^145, 7^143, 8^81, 9^97, 10^69, 11^58, 12^52, 13^33, 14^32, 15^22, 16^16, 17^11, 18^11, 19^3, 20^5, 24^4, 31, 33, 75, 151, 249, 257, 270]
surviving nonsolo ucounts = 4[75, 249, 257, 270]
ids = [918, 1960, 2043, 1043]

====================================================================================

UMI info for barcode AAGACCTAGATGCCAG-1 contig 1 = ACTTTCTGAG...
umi CGAAGGGTAA = 6 reads: -368X +3 -3XX +2 -2XX +1 -2XX +1 -2XX +1 -2XX +3 -1XX +1 -4XX +3 -2XX +35 invalidated
umi CGTTGACTAC = 269 reads: +436 validated

UMI info for barcode AAGACCTAGATGCCAG-1 contig 2 = GAGAGACTGA...
umi TCCGACGTGA = 244 reads: +406 validated
umi TCTGTCTTTA = 259 reads: +406 validated

GOOD CONTIGS

TIG 1[bases=566]
0-35 ==> 66-101 on |195|IGHV4-61|5'UTR| [len=101] (mis=0)
35-391 ==> 0-356 on |196|IGHV4-61|L-REGION+V-REGION| [len=356] (mis=26)
400-421 ==> 0-21 on |33|IGHD6-19|D-REGION| [len=21] (mis=2)
420-471 ==> 0-51 on |57|IGHJ5|J-REGION| [len=51] (mis=3)
471-566 ==> 0-95 on |40|IGHG1|C-REGION| [len=884] (mis=1)
junction support: 1 umis using 39 reads
cdr3 = CARHRAGGIAVAATTWFDPW at 380, score = 8 + 7
umis assigned: [918, 1043]
of which 2 are surviving nonsolos
reads assigned: 273
start codons at 35, 79, 341
confident = true

TIG 2[bases=558]
0-37 ==> 0-37 on |391|IGLV9-49|5'UTR| [len=37] (mis=0)
37-406 ==> 0-369 on |392|IGLV9-49|L-REGION+V-REGION| [len=369] (mis=7)
405-443 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=2)
443-558 ==> 0-115 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 2 umis using 56 reads
cdr3 = CGADHGSGNNFVWVF at 370, score = 5 + 8
umis assigned: [1960, 2043]
of which 2 are surviving nonsolos
reads assigned: 495
start codons at 37, 188, 235, 353, 383
confident = true
now this is a cell
paired!

GTGTATTACTGTGCGAGACATCGGGCTGGCGGTATAGCAGTGGCTGCTACGACCTGGTTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG <==> CAGGAAGAAGATGAGAGTGACTACCACTGTGGGGCAGACCATGGCAGTGGGAACAACTTCGTGTGGGTGTTCGGCGGAGGGACCAAGTTGACCGTCCTAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 3.78 = AAGACCTAGCAGATCG-1

using 80 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 4[4, 6, 8, 62]
surviving nonsolo ucounts = 1[62]
ids = [0]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 3.82 = AAGACCTAGCGTTTAC-1

using 26 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 1[23]
surviving nonsolo ucounts = 1[23]
ids = [3]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 3.83 = AAGACCTAGCTGAAAT-1

using 306 reads

====================================================================================

graph has 84 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 2[4, 298]
surviving nonsolo ucounts = 1[298]
ids = [4]

====================================================================================

UMI info for barcode AAGACCTAGCTGAAAT-1 contig 1 = ATCAGTCCCA...
umi GTGCCTTCGG = 285 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=497]
0-23 ==> 4-27 on |222|IGKV1-17|5'UTR| [len=27] (mis=0)
23-374 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
373-411 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
411-497 ==> 0-86 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 43 reads
cdr3 = CLQYSSSPWTF at 350, score = 9 + 8
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 282
start codons at 23, 29, 98, 234, 453
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 3.85 = AAGACCTAGGCTCATT-1

using 20 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 3.100 = AAGACCTAGTTGTCGT-1

using 324 reads

====================================================================================

graph has 136 edges initially, 4 edges after simplification

total ucounts = 6
nonsolo ucounts = 3[6, 89, 226]
surviving nonsolo ucounts = 2[89, 226]
ids = [1, 3]

====================================================================================

UMI info for barcode AAGACCTAGTTGTCGT-1 contig 1 = AGGAATCAGT...
umi GGCGGCATAG = 220 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=467]
0-27 ==> 0-27 on |222|IGKV1-17|5'UTR| [len=27] (mis=0)
27-378 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
377-415 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
415-467 ==> 0-52 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 33 reads
cdr3 = CLQYSSSPWTF at 354, score = 9 + 8
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 215
start codons at 27, 33, 102, 238, 457
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 3.102 = AAGACCTCAAACTGCT-1

using 1310 reads

====================================================================================

graph has 404 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[255, 396, 657]
surviving nonsolo ucounts = 2[396, 657]
ids = [0, 2]

====================================================================================

UMI info for barcode AAGACCTCAAACTGCT-1 contig 1 = GGGGAGGAAC...
umi AATAATTACA = 394 reads: +382 validated
umi GAGGCATGCG = 654 reads: -173X +209 invalidated
umi TAATGGCCGA = 52 reads: -234 +2 -1XX +1 -1XX +1 -1XX +1 -1XX +11 -1XX +14 -1XX +23 -2XX +2 -1XX +8 -1XX +1 -1XX +20 -3XX +1 -9XX +5 -1XX +2 -1XX +2 -1XX +5 -1XX +14 -1XX +3 -1XX +3 invalidated

GOOD CONTIGS

TIG 1[bases=554]
0-36 ==> 11-47 on |278|IGKV3-11|5'UTR| [len=47] (mis=0)
36-381 ==> 0-345 on |279|IGKV3-11|L-REGION+V-REGION| [len=345] (mis=5)
380-418 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=5)
418-554 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 2 umis using 233 reads
cdr3 = CQQRSNWPPTF at 357, score = 9 + 8
umis assigned: [0, 2, 4]
of which 2 are surviving nonsolos
reads assigned: 1088
start codons at 36, 241, 244, 460
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 3.105 = AAGACCTCAAGGACAC-1

using 824 reads

====================================================================================

graph has 256 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[2, 389, 432]
surviving nonsolo ucounts = 2[389, 432]
ids = [0, 2]

====================================================================================

UMI info for barcode AAGACCTCAAGGACAC-1 contig 1 = GAGAAGAGCT...
umi ACGTGGCAAT = 387 reads: +385 validated
umi GATAGGAGTA = 431 reads: +385 validated

GOOD CONTIGS

TIG 1[bases=556]
0-35 ==> 17-52 on |282|IGKV3-20|5'UTR| [len=52] (mis=0)
35-383 ==> 0-348 on |283|IGKV3-20|L-REGION+V-REGION| [len=348] (mis=4)
381-420 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=5)
420-556 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 2 umis using 115 reads
cdr3 = CQQYGSSPVTF at 359, score = 9 + 8
umis assigned: [0, 2]
of which 2 are surviving nonsolos
reads assigned: 807
start codons at 35, 243, 246, 369, 462
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 3.107 = AAGACCTCACACAGAG-1

using 397 reads

====================================================================================

graph has 196 edges initially, 4 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[7, 53, 335]
surviving nonsolo ucounts = 2[53, 335]
ids = [1, 4]

====================================================================================

UMI info for barcode AAGACCTCACACAGAG-1 contig 1 = AGTCAGTCCC...
umi TTTCATCACG = 341 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=548]
0-24 ==> 7-31 on |226|IGKV1-33|5'UTR| [len=31] (mis=0)
24-375 ==> 0-351 on |227|IGKV1-33|L-REGION+V-REGION| [len=351] (mis=10)
373-412 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=3)
412-548 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 62 reads
cdr3 = CQQYDNLPYTF at 351, score = 9 + 8
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 333
start codons at 24, 30, 86, 99, 238, 454
confident = false

REJECT CONTIGS

TIG 1[bases=342]
0-307 ==> 49-356 on |321|IGLV1-40|L-REGION+V-REGION| [len=356] (mis=12)
307-342 ==> 0-35 on |309|IGLJ1|J-REGION| [len=38] (mis=0)
cdr3 = CQSYDSSLSGLYVF at 275, score = 7 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 44
start codons at 105, 108, 159, 258, 285, 309
confident = false
not full
VJ delta = 22
not full
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 3.110 = AAGACCTCACATCCGG-1

using 13 reads

====================================================================================

graph has 6 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[13]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 3.129 = AAGACCTCAGCTGCAC-1

using 454 reads

====================================================================================

graph has 156 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 3[3, 9, 437]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 3.148 = AAGACCTGTCCCGACA-1

using 217 reads

====================================================================================

graph has 102 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[2, 215]
surviving nonsolo ucounts = 1[215]
ids = [0]

====================================================================================

UMI info for barcode AAGACCTGTCCCGACA-1 contig 1 = TGGGGAGGAA...
umi CTCCGGAGCC = 216 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=556]
32-383 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=6)
382-420 ==> 0-38 on |215|IGKJ3|J-REGION| [len=38] (mis=0)
420-556 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 33 reads
cdr3 = CLQHKSYPFTF at 359, score = 9 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 210
start codons at 32, 38, 107, 243, 462
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 3.152 = AAGACCTGTCGCATCG-1

using 309 reads

====================================================================================

graph has 94 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 3[2^2, 301]
surviving nonsolo ucounts = 1[301]
ids = [1]

====================================================================================

UMI info for barcode AAGACCTGTCGCATCG-1 contig 1 = ACAAGAGGCA...
umi ATAAGGGATT = 299 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=567]
0-31 ==> 20-51 on |320|IGLV1-40|5'UTR| [len=51] (mis=0)
31-387 ==> 0-356 on |321|IGLV1-40|L-REGION+V-REGION| [len=356] (mis=0)
387-425 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=2)
425-567 ==> 0-142 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 46 reads
cdr3 = CQSYDSSLSGSGVF at 355, score = 8 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 296
start codons at 31, 185, 188, 239, 338, 365
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 3.154 = AAGACCTGTCGGCTCA-1

using 307 reads

====================================================================================

graph has 92 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 4[3, 5, 7, 289]
surviving nonsolo ucounts = 1[289]
ids = [2]

====================================================================================

UMI info for barcode AAGACCTGTCGGCTCA-1 contig 1 = AGAGCTGCTC...
umi CGGTTATCAC = 273 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=516]
0-31 ==> 21-52 on |282|IGKV3-20|5'UTR| [len=52] (mis=0)
31-379 ==> 0-348 on |283|IGKV3-20|L-REGION+V-REGION| [len=348] (mis=2)
381-419 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=0)
419-516 ==> 0-97 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 46 reads
cdr3 = CQQYGSSSGWTF at 355, score = 9 + 8
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 270
start codons at 31, 239, 365, 461
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 3.155 = AAGACCTGTCGTGGCT-1

using 137 reads

====================================================================================

graph has 55 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 1[135]
surviving nonsolo ucounts = 1[135]
ids = [1]

====================================================================================

UMI info for barcode AAGACCTGTCGTGGCT-1 contig 1 = GTCAGTCTCA...
umi ATCCTGCTCT = 130 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=482]
0-23 ==> 24-47 on |230|IGKV1-39|5'UTR| [len=47] (mis=0)
23-374 ==> 0-351 on |231|IGKV1-39|L-REGION+V-REGION| [len=351] (mis=11)
373-411 ==> 0-38 on |217|IGKJ5|J-REGION| [len=38] (mis=0)
411-482 ==> 0-71 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 21 reads
cdr3 = CQQSYRRPITF at 350, score = 8 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 127
start codons at 23, 29, 85, 98, 234, 333, 453
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 3.160 = AAGACCTGTCTCTCTG-1

using 531 reads

====================================================================================

graph has 218 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 2[2, 523]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!
sorting bam, mem = 0.06
running samtools sort -l 8G -o /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk003-ucc6a7a72ce/files/contig_bam_sorted.bam /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk003-ucc6a7a72ce/files/contig_bam.bam

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

4.205 seconds used processing barcodes, peak mem = 0.23
