[stdout]

starting vdj_asm_asm, mem = 0.01
available mem = 828.26 GB
/scratch/etanis/software/cellranger-6.1.2/lib/bin/cr_vdj martian assembly main /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_T_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk88-ucc6a7a72e6 /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_T_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk88-ucc6a7a72e6/files /scratch/etanis/ST-118/multi/dimitri-multi/journal/SC_MULTI_CS.SC_MULTI_CORE.MULTI_GEM_WELL_PROCESSOR.VDJ_T_GEM_WELL_PROCESSOR.SC_VDJ_CONTIG_ASSEMBLER.ASSEMBLE_VDJ.fork0.chnk88.ucc6a7a72e6
n50_n50_rpu = 2159

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.0 = TGACTAGCAAAGCAAT-1

using 66627 reads

====================================================================================

graph has 29413 edges initially, 988 edges after simplification

total ucounts = 1423
nonsolo ucounts = 1261[2^66, 3^47, 4^53, 5^46, 6^26, 7^39, 8^28, 9^30, 10^25, 11^25, 12^23, 13^22, 14^31, 15^14, 16^21, 17^21, 18^23, 19^15, 20^21, 21^22, 22^10, 23^14, 24^23, 25^15, 26^10, 27^13, 28^12, 29^17, 30^7, 31^12, 32^8, 33^6, 34^9, 35^9, 36^9, 37^10, 38^15, 39^12, 40^5, 41^9, 42^7, 43^7, 44^14, 45^10, 46^4, 47^8, 48^16, 49^10, 50^8, 51^10, 52^7, 53^4, 54^3, 55^5, 56^3, 57^3, 58^8, 59^6, 60^9, 61^9, 62^11, 63^6, 64^8, 65^6, 66^6, 67^6, 68^8, 69^6, 70^5, 71^6, 72^2, 73^4, 74^2, 75^3, 76^2, 77^6, 78^5, 79^6, 80^3, 81^5, 82^9, 83^5, 84^3, 85^3, 86^5, 87^6, 88^9, 89^6, 90^3, 91^4, 92^4, 93^3, 94, 95^6, 96^4, 97^3, 98^2, 99^5, 100^3, 101, 102^4, 103^4, 104^5, 105^5, 106, 107^2, 108^4, 109^2, 110, 111^5, 112^6, 113^6, 114^2, 115, 117^4, 118, 119^2, 121^3, 122^2, 123^3, 124^3, 126, 127^2, 128, 129, 130, 131, 133, 134, 135^2, 136, 137, 140, 141, 143^2, 144, 146, 147, 182, 206, 1047, 1640, 2045, 2730, 2898, 3034, 3642, 3730]
surviving nonsolo ucounts = 10[13, 59, 1047, 1640, 2045, 2730, 2898, 3034, 3642, 3730]
ids = [305, 671, 463, 105, 892, 142, 222, 752, 323, 178]

====================================================================================

UMI info for barcode TGACTAGCAAAGCAAT-1 contig 1 = TGGGGTCTTT...
umi AATGGATCCG = 1368 reads: -15X +1 -4XX +1 -2XX +1 -4XX +1 -1XX +1 -2XX +1 -1XX +1 -1XX +1 -9XX +350 invalidated
umi ACCTCCGCGC = 2748 reads: +397 validated
umi ACTGTCAATT = 3756 reads: -24X +1 -1XX +371 invalidated
umi AGGCTGCTAG = 2897 reads: +397 validated
umi AGTGGCTGCA = 1 reads: -394X +1 -2X invalidated
umi ATTATACTTT = 3595 reads: +397 validated
umi CATGTTGTAT = 1038 reads: +397 validated
umi CTGGAGGTCC = 1 reads: -392 +1 -3X +1 invalidated
umi CTTTAAATTA = 3040 reads: +397 validated
umi GGATTACATT = 2051 reads: +397 validated
umi GTGCGATTCT = 1 reads: -394X +1 -2X invalidated
umi TCCTAATCGA = 1 reads: -394X +1 -2X invalidated
umi TTTGGCACCT = 1 reads: -393 +1 -1X +1 -1X invalidated

GOOD CONTIGS

TIG 1[bases=472]
23-359 ==> 0-336 on |613|TRBV28|L-REGION+V-REGION| [len=344] (mis=0)
372-420 ==> 0-48 on |548|TRBJ2-5|J-REGION| [len=48] (mis=3)
420-472 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 8 umis using 1545 reads
cdr3 = CASERVDREETQYF at 350, score = 6 + 7
umis assigned: [105, 142, 178, 222, 244, 323, 463, 719, 752, 892] and 3 others
of which 8 are surviving nonsolos
reads assigned: 20112
start codons at 23, 81, 145, 158, 165, 173, 228, 231, 239, 341
confident = true
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2 = TGACTAGCAAATTGCC-1

using 38 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.3 = TGACTAGCAAGACGTG-1

using 53 reads

====================================================================================

graph has 62 edges initially, 2 edges after simplification

total ucounts = 16
nonsolo ucounts = 9[2^6, 6, 7, 21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.4 = TGACTAGCAAGCCATT-1

using 146 reads

====================================================================================

graph has 88 edges initially, 4 edges after simplification

total ucounts = 7
nonsolo ucounts = 4[19, 26, 29, 69]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.5 = TGACTAGCAAGCCTAT-1

using 67 reads

====================================================================================

graph has 86 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 7[2, 4, 5, 6, 9, 12, 28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.6 = TGACTAGCAAGCTGAG-1

using 62 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[62]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.8 = TGACTAGCAAGGACAC-1

using 252 reads

====================================================================================

graph has 293 edges initially, 16 edges after simplification

total ucounts = 18
nonsolo ucounts = 16[2^2, 3^2, 4, 6, 7, 8^2, 9, 14, 15, 25, 26, 46, 72]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.9 = TGACTAGCAAGGACTG-1

using 78 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[78]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.11 = TGACTAGCAATGCCAT-1

using 142 reads

====================================================================================

graph has 268 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 7[2, 3^3, 4, 44, 80]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.12 = TGACTAGCAATGGACG-1

using 107 reads

====================================================================================

graph has 100 edges initially, 4 edges after simplification

total ucounts = 14
nonsolo ucounts = 11[2^7, 3^2, 26, 58]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.16 = TGACTAGCACATCTTT-1

using 58 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[58]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.23 = TGACTAGCAGCCTTTC-1

using 79 reads

====================================================================================

graph has 56 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[79]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.25 = TGACTAGCAGCTTAAC-1

using 43 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 6[2, 4, 5, 6, 11, 13]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.26 = TGACTAGCAGGACCCT-1

using 43 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[43]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.29 = TGACTAGCAGGTCTCG-1

using 84 reads

====================================================================================

graph has 42 edges initially, 4 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[33, 51]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.30 = TGACTAGCAGGTTTCA-1

using 24 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.31 = TGACTAGCAGTACACT-1

using 82 reads

====================================================================================

graph has 84 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[82]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.33 = TGACTAGCAGTATAAG-1

using 30 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.34 = TGACTAGCAGTCACTA-1

using 48 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[48]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.37 = TGACTAGCAGTTCCCT-1

using 33 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.38 = TGACTAGCATAAAGGT-1

using 42 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[42]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.39 = TGACTAGCATACCATG-1

using 116 reads

====================================================================================

graph has 100 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[116]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.40 = TGACTAGCATAGGATA-1

using 6842 reads

====================================================================================

graph has 5065 edges initially, 198 edges after simplification

total ucounts = 561
nonsolo ucounts = 434[2^57, 3^25, 4^36, 5^28, 6^11, 7^21, 8^18, 9^17, 10^15, 11^17, 12^12, 13^13, 14^13, 15^9, 16^9, 17^8, 18^9, 19^8, 20^12, 21^7, 22^7, 23^9, 24^7, 25^4, 26^6, 27^5, 28^5, 29^9, 30^5, 31, 32^3, 33^4, 34, 35^2, 36, 37^4, 40^2, 41, 44^2, 46^2, 48, 49, 50, 54, 63, 71, 97, 149, 884]
surviving nonsolo ucounts = 2[30, 884]
ids = [324, 37]

====================================================================================

UMI info for barcode TGACTAGCATAGGATA-1 contig 1 = GATCTTAATT...
umi ACTTGGACCG = 820 reads: +388 validated
umi GGCACCAGTT = 31 reads: -1 +2 -1 +3 -1 +255 -2 +80 -1 +42 non-validated
umi TAACTTGATC = 1 reads: -374 +1 -7X +2 -2X +1 -1X invalidated

GOOD CONTIGS

TIG 1[bases=453]
0-23 ==> 0-23 on |463|TRAV13-1|5'UTR| [len=23] (mis=0)
23-360 ==> 0-337 on |464|TRAV13-1|L-REGION+V-REGION| [len=337] (mis=5)
356-411 ==> 0-55 on |406|TRAJ21|J-REGION| [len=55] (mis=0)
411-453 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 1 umis using 27 reads
cdr3 = CAAYNFNKFYF at 347, score = 9 + 7
umis assigned: [37, 324, 379]
of which 2 are surviving nonsolos
reads assigned: 836
start codons at 23, 78, 87, 237, 399
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.43 = TGACTAGCATCCTTGC-1

using 11 reads

====================================================================================

graph has 2 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[11]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.44 = TGACTAGCATCGGTTA-1

using 57 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[57]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.45 = TGACTAGCATCGTCGG-1

using 217 reads

====================================================================================

graph has 68 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 6[2, 5, 6, 7, 52, 145]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.46 = TGACTAGCATGAAGTA-1

using 18 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[17]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.47 = TGACTAGCATGCAATC-1

using 61 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 3[6, 7, 48]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.49 = TGACTAGCATGCCTAA-1

using 62 reads

====================================================================================

graph has 48 edges initially, 4 edges after simplification

total ucounts = 10
nonsolo ucounts = 8[3^2, 5^2, 9^2, 11, 15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.53 = TGACTAGCATTCTCAT-1

using 35 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.55 = TGACTAGGTAAACACA-1

using 93 reads

====================================================================================

graph has 94 edges initially, 4 edges after simplification

total ucounts = 11
nonsolo ucounts = 9[3^3, 5, 6, 7, 15, 18, 31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.56 = TGACTAGGTAAACGCG-1

using 286 reads

====================================================================================

graph has 156 edges initially, 6 edges after simplification

total ucounts = 9
nonsolo ucounts = 9[2^2, 4, 7, 15, 28, 67, 69, 92]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.57 = TGACTAGGTAACGTTC-1

using 12 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[12]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.62 = TGACTAGGTACCAGTT-1

using 62 reads

====================================================================================

graph has 64 edges initially, 2 edges after simplification

total ucounts = 18
nonsolo ucounts = 11[2, 3^4, 4, 5^2, 7, 10^2]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.67 = TGACTAGGTATCGCAT-1

using 25345 reads

====================================================================================

graph has 18619 edges initially, 693 edges after simplification

total ucounts = 1902
nonsolo ucounts = 1476[2^193, 3^161, 4^106, 5^87, 6^82, 7^58, 8^66, 9^64, 10^53, 11^40, 12^57, 13^48, 14^46, 15^37, 16^26, 17^24, 18^20, 19^25, 20^28, 21^27, 22^15, 23^23, 24^14, 25^15, 26^7, 27^13, 28^12, 29^15, 30^9, 31^5, 32^6, 33^8, 34^11, 35^4, 36^5, 37^8, 38^3, 39^2, 40^2, 41^4, 42, 43^2, 44^2, 46, 47^3, 48, 49^3, 50, 51^2, 53, 54^2, 57^2, 58, 59^4, 60, 61, 63, 64, 67^2, 69^2, 70^3, 72, 74, 78, 85, 90, 93, 101, 102, 107, 6893]
surviving nonsolo ucounts = 6[4^2, 5, 6, 8, 6893]
ids = [1692, 1757, 1682, 1697, 1655, 1182]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.68 = TGACTAGGTATTAGCC-1

using 31 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.70 = TGACTAGGTCACTGGC-1

using 28 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[5, 23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.74 = TGACTAGGTCTTGTCC-1

using 82 reads

====================================================================================

graph has 52 edges initially, 2 edges after simplification

total ucounts = 12
nonsolo ucounts = 8[2^2, 3^2, 5, 6, 19, 38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.75 = TGACTAGGTGGTAACG-1

using 35 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.80 = TGACTAGGTGTTGGGA-1

using 82 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[82]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.81 = TGACTAGGTTACGACT-1

using 61 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[61]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.82 = TGACTAGGTTACGCGC-1

using 2363 reads

====================================================================================

graph has 2144 edges initially, 88 edges after simplification

total ucounts = 339
nonsolo ucounts = 220[2^54, 3^29, 4^23, 5^17, 6^8, 7^6, 8^12, 9^4, 10^5, 11^4, 12^3, 13, 14^6, 15^2, 16^2, 17^3, 18^3, 19^3, 20^3, 21^3, 22, 23^2, 24^3, 25, 26, 27^2, 29, 31, 34^2, 37^2, 38, 39, 40, 42^2, 44^3, 50, 55, 56, 67, 68]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.84 = TGACTAGGTTCCATGA-1

using 20 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.87 = TGACTAGGTTCGCTAA-1

using 25 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 2[2, 20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.92 = TGACTAGGTTTGTTTC-1

using 171 reads

====================================================================================

graph has 112 edges initially, 10 edges after simplification

total ucounts = 18
nonsolo ucounts = 13[2^3, 3, 4, 6, 10, 13, 14, 15, 20, 22, 53]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.96 = TGACTAGTCACAGGCC-1

using 33 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 5[2^3, 3, 20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.100 = TGACTAGTCAGTGTTG-1

using 78 reads

====================================================================================

graph has 74 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 6[5, 9, 10^2, 19, 20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.102 = TGACTAGTCATAGCAC-1

using 43 reads

====================================================================================

graph has 52 edges initially, 4 edges after simplification

total ucounts = 14
nonsolo ucounts = 7[2^2, 3, 4, 5, 8, 12]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.107 = TGACTAGTCCAAGCCG-1

using 27 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.109 = TGACTAGTCCATTCTA-1

using 25 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.110 = TGACTAGTCCCAACGG-1

using 139 reads

====================================================================================

graph has 92 edges initially, 10 edges after simplification

total ucounts = 13
nonsolo ucounts = 11[2, 3^2, 5, 7^2, 12, 13, 16, 19, 50]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.115 = TGACTAGTCCGTAGGC-1

using 53 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[4, 6, 42]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.116 = TGACTAGTCGAATCCA-1

using 38 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 4[3, 5^2, 21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.119 = TGACTAGTCGTCCAGG-1

using 70 reads

====================================================================================

graph has 96 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 5[2, 3, 5, 20, 40]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.122 = TGACTAGTCTAACTTC-1

using 34 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 4[2, 3, 13^2]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.128 = TGACTAGTCTGACCTC-1

using 27 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 4[2^2, 3, 16]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.130 = TGACTAGTCTGGCGTG-1

using 56 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[56]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.136 = TGACTTTAGAATAGGG-1

using 233 reads

====================================================================================

graph has 120 edges initially, 6 edges after simplification

total ucounts = 22
nonsolo ucounts = 19[2^5, 4^2, 5^2, 7, 9^2, 11^3, 17, 20, 23, 84]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.137 = TGACTTTAGAATTGTG-1

using 257 reads

====================================================================================

graph has 102 edges initially, 4 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[59, 198]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.138 = TGACTTTAGACAATAC-1

using 286 reads

====================================================================================

graph has 200 edges initially, 6 edges after simplification

total ucounts = 21
nonsolo ucounts = 17[2^2, 5^2, 8, 9, 10^2, 11, 13, 14, 16, 21, 25^2, 49, 57]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.140 = TGACTTTAGAGCTGGT-1

using 24 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.141 = TGACTTTAGAGGACGG-1

using 66 reads

====================================================================================

graph has 60 edges initially, 2 edges after simplification

total ucounts = 13
nonsolo ucounts = 11[2^3, 3^2, 4^2, 6, 9, 12, 17]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.142 = TGACTTTAGAGTCGGT-1

using 137 reads

====================================================================================

graph has 148 edges initially, 4 edges after simplification

total ucounts = 27
nonsolo ucounts = 16[2^2, 3, 4^3, 5^2, 6, 7, 8, 9, 10, 13, 18, 26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.144 = TGACTTTAGATAGGAG-1

using 427 reads

====================================================================================

graph has 244 edges initially, 6 edges after simplification

total ucounts = 36
nonsolo ucounts = 30[2^7, 3^4, 5, 6^3, 7, 8^2, 9, 13^3, 15, 17, 21, 23, 34, 55, 59, 77]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.145 = TGACTTTAGATATGGT-1

using 138 reads

====================================================================================

graph has 136 edges initially, 6 edges after simplification

total ucounts = 27
nonsolo ucounts = 14[2, 3^3, 4^3, 5, 7, 10, 13, 14, 15, 38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.146 = TGACTTTAGATCCGAG-1

using 182 reads

====================================================================================

graph has 100 edges initially, 4 edges after simplification

total ucounts = 20
nonsolo ucounts = 12[2, 3, 5^3, 6^2, 10, 15, 19, 47, 51]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.147 = TGACTTTAGATCGATA-1

using 50 reads

====================================================================================

graph has 50 edges initially, 6 edges after simplification

total ucounts = 12
nonsolo ucounts = 7[2, 3^2, 5, 6, 11, 15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.154 = TGACTTTAGCTAACTC-1

using 84 reads

====================================================================================

graph has 96 edges initially, 2 edges after simplification

total ucounts = 15
nonsolo ucounts = 10[3^3, 5^2, 9^2, 10^2, 22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.156 = TGACTTTAGCTCTCGG-1

using 104 reads

====================================================================================

graph has 34 edges initially, 4 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[42, 62]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.157 = TGACTTTAGCTGATAA-1

using 90 reads

====================================================================================

graph has 64 edges initially, 4 edges after simplification

total ucounts = 21
nonsolo ucounts = 12[2^2, 3, 4^3, 5, 6, 8, 10, 12, 21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.158 = TGACTTTAGCTGGAAC-1

using 54 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[54]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.161 = TGACTTTAGCTTCGCG-1

using 102 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 6[2, 4^3, 43^2]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.162 = TGACTTTAGGACAGCT-1

using 82 reads

====================================================================================

graph has 70 edges initially, 4 edges after simplification

total ucounts = 16
nonsolo ucounts = 13[2, 3^3, 4^3, 5^3, 7, 13, 21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.163 = TGACTTTAGGACTGGT-1

using 24 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.164 = TGACTTTAGGAGCGAG-1

using 106 reads

====================================================================================

graph has 134 edges initially, 2 edges after simplification

total ucounts = 15
nonsolo ucounts = 8[2, 3, 4^2, 5, 7, 30, 44]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.165 = TGACTTTAGGATCGCA-1

using 129 reads

====================================================================================

graph has 144 edges initially, 4 edges after simplification

total ucounts = 13
nonsolo ucounts = 10[3^2, 4^2, 5^4, 37, 55]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.167 = TGACTTTAGGGCTCTC-1

using 74 reads

====================================================================================

graph has 52 edges initially, 4 edges after simplification

total ucounts = 8
nonsolo ucounts = 8[2, 3^2, 4, 6, 8, 13, 35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.168 = TGACTTTAGGTAGCTG-1

using 92 reads

====================================================================================

graph has 78 edges initially, 4 edges after simplification

total ucounts = 20
nonsolo ucounts = 13[2, 3^2, 4^2, 5, 6, 7^3, 8, 14, 15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.169 = TGACTTTAGGTGATAT-1

using 41 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[41]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.171 = TGACTTTAGGTGCTAG-1

using 29 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.173 = TGACTTTAGTACGTAA-1

using 194 reads

====================================================================================

graph has 156 edges initially, 10 edges after simplification

total ucounts = 22
nonsolo ucounts = 16[2, 3, 4^3, 5, 7, 8, 9, 10, 16, 17, 19, 20, 29, 31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.179 = TGACTTTAGTGCCAGA-1

using 175 reads

====================================================================================

graph has 116 edges initially, 2 edges after simplification

total ucounts = 14
nonsolo ucounts = 11[2^4, 5, 6, 8, 10, 14, 48, 73]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.180 = TGACTTTAGTGCGTGA-1

using 46 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[46]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.181 = TGACTTTAGTGTCTCA-1

using 33 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.182 = TGACTTTAGTGTGGCA-1

using 205 reads

====================================================================================

graph has 102 edges initially, 4 edges after simplification

total ucounts = 21
nonsolo ucounts = 15[3^3, 5, 6, 7, 10, 11^2, 14, 15, 17, 23, 34, 37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.183 = TGACTTTAGTTATCGC-1

using 304 reads

====================================================================================

graph has 164 edges initially, 2 edges after simplification

total ucounts = 33
nonsolo ucounts = 28[2^6, 3^2, 5^3, 6, 7^2, 8^2, 9, 10, 11, 12, 13^2, 16, 17, 20, 21, 41, 47]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.194 = TGACTTTCAAGCTGTT-1

using 15057 reads

====================================================================================

graph has 12823 edges initially, 472 edges after simplification

total ucounts = 1719
nonsolo ucounts = 1250[2^243, 3^146, 4^91, 5^91, 6^79, 7^55, 8^57, 9^42, 10^42, 11^27, 12^18, 13^23, 14^25, 15^24, 16^15, 17^11, 18^24, 19^10, 20^8, 21^12, 22^16, 23^13, 24^9, 25^12, 26^6, 27^3, 28^11, 29^4, 30^6, 31^12, 32^4, 33^10, 34^8, 35^5, 36^6, 37^4, 38^5, 39^6, 40^3, 41^5, 42^6, 43^7, 44^2, 45^2, 46, 47^2, 48^5, 49, 50^2, 52, 53^4, 56^2, 58^2, 59, 61^3, 64, 65, 68, 69^2, 70, 71^2, 72^2, 73, 75, 77, 81, 82, 87, 94, 97]
surviving nonsolo ucounts = 2[4, 42]
ids = [607, 1216]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.198 = TGACTTTCAATAAGCA-1

using 79 reads

====================================================================================

graph has 90 edges initially, 4 edges after simplification

total ucounts = 17
nonsolo ucounts = 13[2^3, 3, 4^3, 6^2, 8, 9, 11, 14]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.201 = TGACTTTCAATCAGAA-1

using 86 reads

====================================================================================

graph has 75 edges initially, 2 edges after simplification

total ucounts = 21
nonsolo ucounts = 13[2^2, 3^4, 5, 6, 7, 8, 10, 11, 15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.204 = TGACTTTCACAACGCC-1

using 3075 reads

====================================================================================

graph has 2658 edges initially, 88 edges after simplification

total ucounts = 357
nonsolo ucounts = 272[2^54, 3^25, 4^18, 5^22, 6^13, 7^13, 8^9, 9^9, 10^12, 11^11, 12^8, 13^7, 14^9, 15^4, 16^7, 17^4, 18^6, 19^4, 20^2, 21^7, 22, 23, 24^2, 26, 27, 28^2, 29, 30, 34^2, 35^2, 36, 39, 40, 44^2, 45^2, 49, 51, 53, 57, 58, 66, 112]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.206 = TGACTTTCACAGTCGC-1

using 22 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.211 = TGACTTTCACCAGGTC-1

using 34 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.212 = TGACTTTCACCAGTTA-1

using 97 reads

====================================================================================

graph has 58 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[97]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.214 = TGACTTTCACCGTTGG-1

using 105 reads

====================================================================================

graph has 62 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 9[2, 3, 4^2, 6, 10, 19, 28^2]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.216 = TGACTTTCACGAAGCA-1

using 1196 reads

====================================================================================

graph has 308 edges initially, 6 edges after simplification

total ucounts = 13
nonsolo ucounts = 11[2^3, 3, 4, 5, 6, 11^2, 14, 1134]
surviving nonsolo ucounts = 1[1134]
ids = [2]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.217 = TGACTTTCACGACTCG-1

using 107 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[107]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.219 = TGACTTTCACGGCCAT-1

using 60 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[59]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.220 = TGACTTTCACGGCTAC-1

using 15060 reads

====================================================================================

graph has 8405 edges initially, 190 edges after simplification

total ucounts = 1026
nonsolo ucounts = 744[2^113, 3^91, 4^51, 5^53, 6^58, 7^41, 8^40, 9^35, 10^23, 11^33, 12^19, 13^18, 14^14, 15^12, 16^10, 17^13, 18^9, 19^6, 20^9, 21^6, 22^8, 23^4, 24^5, 25, 26^9, 27^3, 28^2, 29^4, 31^6, 32^4, 33^3, 34^4, 35, 36^3, 37^2, 38^4, 39, 40^5, 41, 42, 43, 44^2, 45, 46^2, 48, 50, 51^3, 56, 58, 61, 62, 68, 94, 100, 6988]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================

REJECT CONTIGS

TIG 1[bases=307]
5-78 ==> 727-800 on segment before TRAJ12 exon 1 [len=800] (mis=0)
201-265 ==> 0-64 on |395|TRAJ10|J-REGION| [len=64] (mis=0)
265-307 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
umis assigned: [761]
of which 0 are surviving nonsolos
reads assigned: 6912
start codons at 24, 33, 80
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.224 = TGACTTTCACTTAACG-1

using 20 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.225 = TGACTTTCACTTGGAT-1

using 97 reads

====================================================================================

graph has 104 edges initially, 4 edges after simplification

total ucounts = 13
nonsolo ucounts = 9[3^2, 5, 6, 8, 10, 14, 17, 27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.226 = TGACTTTCAGATGAGC-1

using 153 reads

====================================================================================

graph has 80 edges initially, 2 edges after simplification

total ucounts = 15
nonsolo ucounts = 11[2, 3^3, 5^2, 12, 19, 20, 30, 47]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.228 = TGACTTTCAGCTGCAC-1

using 139 reads

====================================================================================

graph has 70 edges initially, 4 edges after simplification

total ucounts = 10
nonsolo ucounts = 8[2^2, 3, 5, 15, 18, 34, 58]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.229 = TGACTTTCAGCTGCTG-1

using 75 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[75]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.232 = TGACTTTCAGGTTTCA-1

using 25354 reads

====================================================================================

graph has 12678 edges initially, 326 edges after simplification

total ucounts = 1286
nonsolo ucounts = 1001[2^136, 3^102, 4^80, 5^56, 6^60, 7^58, 8^38, 9^42, 10^27, 11^27, 12^33, 13^18, 14^26, 15^19, 16^26, 17^18, 18^18, 19^22, 20^16, 21^12, 22^5, 23^15, 24^17, 25^12, 26^9, 27^10, 28^4, 29^5, 30^6, 31^4, 32^4, 33^7, 34^8, 35^3, 36^4, 37^3, 38^5, 39, 40^3, 41^2, 42, 43^3, 44, 46^3, 47^3, 49, 50^3, 51, 52^3, 54, 55, 56, 57, 58, 59, 60, 61, 65, 66, 74, 84, 90, 109, 140, 169, 1077, 1483, 1493, 1938, 6763]
surviving nonsolo ucounts = 9[3, 34, 140, 169, 1077, 1483, 1493, 1938, 6763]
ids = [552, 1227, 1150, 149, 1272, 336, 220, 756, 938]

====================================================================================

UMI info for barcode TGACTTTCAGGTTTCA-1 contig 1 = TGGGCCTGCT...
umi AGAACAACGT = 134 reads: -365 +35 non-validated
umi ATCACCGCAA = 1480 reads: +400 validated
umi GGATGCCTCA = 1927 reads: +400 validated

UMI info for barcode TGACTTTCAGGTTTCA-1 contig 2 = GCCAGGTTCA...
umi CAGATGCAGC = 1488 reads: +400 validated
umi TTGTTTAGTG = 1 reads: -389X +1 -1X +2 -1X +1 -1X +2 -1X +1 invalidated
umi TTTGTGGTCC = 1082 reads: +400 validated

GOOD CONTIGS

TIG 1[bases=473]
21-368 ==> 0-347 on |592|TRBV2|L-REGION+V-REGION| [len=347] (mis=3)
360-372 ==> 0-12 on |535|TRBD1|D-REGION| [len=12] (mis=2)
374-421 ==> 0-47 on |550|TRBJ2-7|J-REGION| [len=47] (mis=4)
421-473 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 2 umis using 230 reads
cdr3 = CASRTAGLQHEQYF at 351, score = 6 + 8
umis assigned: [149, 220, 756]
of which 3 are surviving nonsolos
reads assigned: 3486
start codons at 21, 38, 120, 232, 262, 289, 342
confident = true

TIG 2[bases=517]
0-75 ==> 0-75 on |467|TRAV14/DV4|5'UTR| [len=75] (mis=3)
75-425 ==> 0-350 on |468|TRAV14/DV4|L-REGION+V-REGION| [len=350] (mis=9)
419-475 ==> 0-56 on |436|TRAJ49|J-REGION| [len=56] (mis=2)
475-517 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 2 umis using 112 reads
cdr3 = CAMRVTGNQFYF at 408, score = 7 + 7
umis assigned: [336, 1248, 1272]
of which 2 are surviving nonsolos
reads assigned: 2520
start codons at 75, 165, 208, 229, 267, 295, 307, 399, 414
confident = true

REJECT CONTIGS

TIG 1[bases=339]
5-234 ==> 211-440 on segment before TRBJ1-6 exon 1 [len=440] (mis=0)
234-287 ==> 0-53 on |541|TRBJ1-6|J-REGION| [len=53] (mis=0)
287-339 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
umis assigned: [938]
of which 1 are surviving nonsolos
reads assigned: 6687
start codons at 28, 34, 73, 85, 129
confident = false
did not find CDR3
now this is a cell
paired!

GCTTCACAACTGGGGGACTCAGCAATGTATTTCTGTGCAATGAGGGTCACCGGTAACCAGTTCTATTTTGGGACAGGGACAAGTTTGACGGTCATTCCAA <==> ACAAAGCTGGAGGACTCAGCCATGTACTTCTGTGCCAGCAGGACAGCGGGCCTCCAGCACGAGCAGTACTTCGGGCCGGGCACCAGGCTCACGGTCACAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.236 = TGACTTTCAGTCTTCC-1

using 95 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 5[2, 7, 12, 27, 47]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.239 = TGACTTTCATACTCTT-1

using 261 reads

====================================================================================

graph has 200 edges initially, 8 edges after simplification

total ucounts = 48
nonsolo ucounts = 31[2^4, 3^7, 4^3, 5, 6^2, 8^5, 9^2, 10, 11, 12^2, 15, 32, 36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.242 = TGACTTTCATCCGTGG-1

using 31 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.244 = TGACTTTCATCTACGA-1

using 26 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.245 = TGACTTTCATCTCCCA-1

using 38 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[2, 8, 27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.246 = TGACTTTCATGCAATC-1

using 66 reads

====================================================================================

graph has 60 edges initially, 4 edges after simplification

total ucounts = 12
nonsolo ucounts = 7[2, 3^2, 5, 7, 17, 24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.247 = TGACTTTCATGCTGGC-1

using 92 reads

====================================================================================

graph has 106 edges initially, 4 edges after simplification

total ucounts = 12
nonsolo ucounts = 10[2, 3, 4, 6, 7, 10, 12, 14, 16^2]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.250 = TGACTTTCATTATCTC-1

using 65 reads

====================================================================================

graph has 54 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[3, 62]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.251 = TGACTTTCATTCCTCG-1

using 70 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[70]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.254 = TGACTTTCATTTCACT-1

using 27 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.255 = TGACTTTGTAAAGTCA-1

using 241 reads

====================================================================================

graph has 192 edges initially, 8 edges after simplification

total ucounts = 25
nonsolo ucounts = 19[2, 3^3, 4, 5^2, 7, 8^2, 13^3, 14, 17, 20, 24, 25, 48]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.256 = TGACTTTGTAAGAGGA-1

using 45 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[45]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.257 = TGACTTTGTAAGTAGT-1

using 63 reads

====================================================================================

graph has 71 edges initially, 6 edges after simplification

total ucounts = 11
nonsolo ucounts = 7[2^2, 7^2, 8^2, 25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.258 = TGACTTTGTAATAGCA-1

using 70 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 5[2, 3, 4^2, 53]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.259 = TGACTTTGTACAGTGG-1

using 102 reads

====================================================================================

graph has 76 edges initially, 6 edges after simplification

total ucounts = 15
nonsolo ucounts = 10[2, 3, 5^2, 6, 10, 12, 16, 19^2]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.260 = TGACTTTGTACAGTTC-1

using 38 reads

====================================================================================

graph has 52 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[12, 26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.268 = TGACTTTGTATTCGTG-1

using 66 reads

====================================================================================

graph has 74 edges initially, 2 edges after simplification

total ucounts = 14
nonsolo ucounts = 8[2^2, 5, 8, 9, 10^2, 14]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.269 = TGACTTTGTCAAGCGA-1

using 360 reads

====================================================================================

graph has 382 edges initially, 4 edges after simplification

total ucounts = 60
nonsolo ucounts = 46[2^9, 3^5, 4^4, 5^3, 6^3, 7^4, 8^6, 9, 10^5, 12^2, 13, 14, 26, 52]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.271 = TGACTTTGTCATCCCT-1

using 46 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[46]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.272 = TGACTTTGTCATCGGC-1

using 42 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[42]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.273 = TGACTTTGTCATGCAT-1

using 203 reads

====================================================================================

graph has 124 edges initially, 10 edges after simplification

total ucounts = 20
nonsolo ucounts = 16[2^2, 4^4, 5^2, 6, 7, 10, 14, 15, 29, 40, 48]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.277 = TGACTTTGTCCCTACT-1

using 106 reads

====================================================================================

graph has 118 edges initially, 4 edges after simplification

total ucounts = 19
nonsolo ucounts = 15[2, 3^3, 4^2, 5^2, 6, 8, 9^2, 12, 13, 16]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.279 = TGACTTTGTCGTTGTA-1

using 42 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 6[3, 4^2, 5, 11, 12]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.280 = TGACTTTGTCTAAACC-1

using 326 reads

====================================================================================

graph has 291 edges initially, 6 edges after simplification

total ucounts = 43
nonsolo ucounts = 28[2^3, 3^7, 4^3, 5^2, 6^2, 7, 9, 10^2, 12, 13, 28, 30, 32, 45, 54]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.281 = TGACTTTGTCTAGTCA-1

using 237 reads

====================================================================================

graph has 104 edges initially, 2 edges after simplification

total ucounts = 29
nonsolo ucounts = 19[2^3, 3^4, 4^3, 5, 8^3, 10, 11, 18, 61, 68]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.282 = TGACTTTGTCTCAACA-1

using 38 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[3, 35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.284 = TGACTTTGTCTCCATC-1

using 35 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 3[3, 13, 16]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.285 = TGACTTTGTCTCTTAT-1

using 192 reads

====================================================================================

graph has 106 edges initially, 4 edges after simplification

total ucounts = 16
nonsolo ucounts = 13[2, 3, 5, 6, 7^3, 8, 10, 18, 20, 23, 73]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.288 = TGACTTTGTGATAAAC-1

using 24 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.289 = TGACTTTGTGATGCCC-1

using 54 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[54]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.292 = TGACTTTGTGGACGAT-1

using 33439 reads

====================================================================================

graph has 17027 edges initially, 530 edges after simplification

total ucounts = 1970
nonsolo ucounts = 1471[2^200, 3^118, 4^109, 5^79, 6^88, 7^65, 8^73, 9^76, 10^33, 11^44, 12^43, 13^47, 14^25, 15^33, 16^22, 17^21, 18^25, 19^17, 20^16, 21^18, 22^13, 23^16, 24^11, 25^8, 26^17, 27^11, 28^15, 29^9, 30^14, 31^9, 32^7, 33^10, 34^9, 35^11, 36^6, 37^5, 38^7, 39^3, 40^8, 41^4, 42^5, 43^8, 44^7, 45^4, 46^9, 47^2, 48^4, 49^11, 50^5, 51^7, 53^3, 54^3, 55^6, 56^2, 57^3, 58^4, 59^3, 60, 61^3, 62, 63^2, 64, 65^2, 66^2, 67, 68, 69^2, 71, 72, 73^2, 74, 76^3, 77, 79, 80^2, 82, 84, 87^2, 88, 89, 94, 102, 106, 146, 3119, 8136]
surviving nonsolo ucounts = 3[146, 3119, 8136]
ids = [1726, 123, 1849]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.295 = TGACTTTGTGTGGCTC-1

using 122 reads

====================================================================================

graph has 105 edges initially, 6 edges after simplification

total ucounts = 14
nonsolo ucounts = 12[2, 4^2, 6^2, 7^2, 9, 14, 15, 20, 26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.297 = TGACTTTGTGTTAAGA-1

using 67 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[67]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.298 = TGACTTTGTGTTCTTT-1

using 29 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.300 = TGACTTTGTTAAGACA-1

using 32 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 4[2^2, 9, 12]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.302 = TGACTTTGTTACGGAG-1

using 63 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 2[18, 40]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.305 = TGACTTTGTTATTCTC-1

using 23 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.306 = TGACTTTGTTCAACCA-1

using 76 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[76]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.309 = TGACTTTGTTCCCTTG-1

using 19 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.311 = TGACTTTGTTGATTCG-1

using 59 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[4, 55]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.312 = TGACTTTGTTGATTGC-1

using 29 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.313 = TGACTTTGTTGGAGGT-1

using 37 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.315 = TGACTTTGTTGTACAC-1

using 44 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[44]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.318 = TGACTTTTCAAGATCC-1

using 132 reads

====================================================================================

graph has 174 edges initially, 4 edges after simplification

total ucounts = 18
nonsolo ucounts = 13[2^3, 3^2, 4, 6^3, 11, 12, 19, 51]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.321 = TGACTTTTCACATACG-1

using 39 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[39]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.322 = TGACTTTTCACCATAG-1

using 55 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[55]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.324 = TGACTTTTCACGCGGT-1

using 26 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.325 = TGACTTTTCACGGTTA-1

using 55 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[55]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.326 = TGACTTTTCACTCCTG-1

using 37 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.327 = TGACTTTTCAGGCCCA-1

using 26 reads

====================================================================================

graph has 6 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.329 = TGACTTTTCATATCGG-1

using 81 reads

====================================================================================

graph has 54 edges initially, 2 edges after simplification

total ucounts = 18
nonsolo ucounts = 13[2^3, 3^3, 4, 5^2, 7, 11, 14, 15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.332 = TGACTTTTCATGTAGC-1

using 228 reads

====================================================================================

graph has 164 edges initially, 6 edges after simplification

total ucounts = 25
nonsolo ucounts = 18[2^2, 3^2, 4, 5, 10, 11^2, 12^2, 15^2, 17^2, 24, 29^2]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.333 = TGACTTTTCCAAACAC-1

using 252 reads

====================================================================================

graph has 186 edges initially, 12 edges after simplification

total ucounts = 37
nonsolo ucounts = 18[2^4, 3, 4, 5^3, 6^2, 9, 12, 14^2, 23, 26, 93]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.334 = TGACTTTTCCAATGGT-1

using 1261 reads

====================================================================================

graph has 930 edges initially, 26 edges after simplification

total ucounts = 156
nonsolo ucounts = 109[2^15, 3^15, 4^4, 5^9, 6^7, 7^3, 8^4, 9^8, 10^7, 11^2, 12^5, 13^2, 14^3, 15^3, 16^2, 17, 18^3, 19^2, 21, 22^2, 23, 24, 27, 28, 29, 30, 35^2, 40, 45, 124]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.335 = TGACTTTTCCATGAGT-1

using 50 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[50]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.338 = TGACTTTTCCCTGACT-1

using 14 reads

====================================================================================

graph has 6 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[14]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.339 = TGACTTTTCCGATATG-1

using 153 reads

====================================================================================

graph has 134 edges initially, 4 edges after simplification

total ucounts = 16
nonsolo ucounts = 15[2^2, 3, 4^2, 5, 6, 7, 8, 9^2, 10, 15, 33, 35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.341 = TGACTTTTCCGCATCT-1

using 103 reads

====================================================================================

graph has 70 edges initially, 2 edges after simplification

total ucounts = 16
nonsolo ucounts = 12[2^2, 3^3, 4, 6, 7, 12, 15^2, 27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.343 = TGACTTTTCCGTAGGC-1

using 78 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 14
nonsolo ucounts = 10[2^2, 3, 5, 6, 7, 9, 11, 13, 16]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.345 = TGACTTTTCCTAAGTG-1

using 13 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[13]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.347 = TGACTTTTCCTCGCAT-1

using 141 reads

====================================================================================

graph has 140 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 5[2, 6^2, 9, 114]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.348 = TGACTTTTCCTGTAGA-1

using 84 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 18
nonsolo ucounts = 8[2^2, 3, 4, 11, 12, 17, 23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.351 = TGACTTTTCGACCAGC-1

using 12 reads

====================================================================================

graph has 6 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[12]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.352 = TGACTTTTCGAGAACG-1

using 61 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 13
nonsolo ucounts = 10[2^2, 3^2, 5, 7^3, 8, 14]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.356 = TGACTTTTCGGAGCAA-1

using 224 reads

====================================================================================

graph has 104 edges initially, 6 edges after simplification

total ucounts = 17
nonsolo ucounts = 13[2, 3, 4, 5^2, 10, 12, 13^2, 14, 43, 45, 51]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.358 = TGACTTTTCGTCACGG-1

using 172 reads

====================================================================================

graph has 120 edges initially, 4 edges after simplification

total ucounts = 18
nonsolo ucounts = 12[3^3, 5^2, 8, 10, 13, 14, 27, 36, 39]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.360 = TGACTTTTCGTTTAGG-1

using 158 reads

====================================================================================

graph has 93 edges initially, 4 edges after simplification

total ucounts = 9
nonsolo ucounts = 8[2^2, 3^2, 13, 32, 35, 67]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.361 = TGACTTTTCTAACTTC-1

using 102 reads

====================================================================================

graph has 72 edges initially, 4 edges after simplification

total ucounts = 9
nonsolo ucounts = 8[2, 3^2, 5, 8, 15, 32, 33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.363 = TGACTTTTCTCAAACG-1

using 266 reads

====================================================================================

graph has 192 edges initially, 6 edges after simplification

total ucounts = 29
nonsolo ucounts = 22[2^4, 3, 4^3, 7, 8^2, 10^2, 11, 12, 13, 14, 18^2, 22, 32, 53]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.364 = TGACTTTTCTGAAAGA-1

using 177 reads

====================================================================================

graph has 158 edges initially, 2 edges after simplification

total ucounts = 22
nonsolo ucounts = 17[2^2, 3^3, 4^2, 5, 6, 7, 10^3, 13, 21, 33, 36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.367 = TGACTTTTCTGCTGCT-1

using 92 reads

====================================================================================

graph has 54 edges initially, 2 edges after simplification

total ucounts = 12
nonsolo ucounts = 7[2, 3, 4^3, 19, 51]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.368 = TGACTTTTCTGGAGCC-1

using 162 reads

====================================================================================

graph has 94 edges initially, 2 edges after simplification

total ucounts = 22
nonsolo ucounts = 17[2, 3^5, 4^4, 6^2, 7^2, 17, 36, 45]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.370 = TGACTTTTCTTAACCT-1

using 27 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.371 = TGACTTTTCTTATCTG-1

using 42 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[10, 31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.376 = TGACTTTTCTTGGGTA-1

using 229 reads

====================================================================================

graph has 162 edges initially, 4 edges after simplification

total ucounts = 26
nonsolo ucounts = 22[2^3, 3^2, 4^2, 5, 6^3, 7, 8, 9, 10^2, 12^2, 13, 22, 27, 52]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.379 = TGAGAGGAGAAACCGC-1

using 19 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.380 = TGAGAGGAGAAACCTA-1

using 48 reads

====================================================================================

graph has 54 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 5[2, 4^2, 10, 22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.381 = TGAGAGGAGAAGCCCA-1

using 46 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 4[3, 5, 7, 30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.382 = TGAGAGGAGAAGGGTA-1

using 149 reads

====================================================================================

graph has 118 edges initially, 6 edges after simplification

total ucounts = 13
nonsolo ucounts = 10[2^3, 3, 4, 9, 12, 23, 34, 55]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.385 = TGAGAGGAGACCCACC-1

using 51 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[51]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.394 = TGAGAGGAGCACACAG-1

using 39 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[3, 34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.399 = TGAGAGGAGCGATATA-1

using 53 reads

====================================================================================

graph has 62 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 2[2, 48]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.401 = TGAGAGGAGCTAACAA-1

using 306 reads

====================================================================================

graph has 236 edges initially, 10 edges after simplification

total ucounts = 23
nonsolo ucounts = 15[2, 3^2, 5, 6, 7, 8^2, 17, 19, 22, 28, 49, 52, 69]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.407 = TGAGAGGAGGTCATCT-1

using 15396 reads

====================================================================================

graph has 7775 edges initially, 288 edges after simplification

total ucounts = 740
nonsolo ucounts = 564[2^73, 3^43, 4^35, 5^32, 6^41, 7^36, 8^16, 9^28, 10^11, 11^11, 12^22, 13^5, 14^21, 15^18, 16^20, 17^15, 18^19, 19^11, 20^8, 21^14, 22^4, 23^5, 24^6, 25^3, 26^3, 27^8, 28^2, 29^4, 30^3, 31^4, 32^4, 33^3, 34^2, 35^2, 36^2, 37^2, 38^2, 39^2, 40, 41, 44, 45^3, 52, 53, 54, 56, 57, 60, 64, 68, 69, 70^2, 78, 82, 86, 87, 88^2, 7854]
surviving nonsolo ucounts = 1[7854]
ids = [28]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.413 = TGAGAGGAGTGAAGAG-1

using 62 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[62]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.416 = TGAGAGGAGTGTGAAT-1

using 67 reads

====================================================================================

graph has 90 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 9[2^2, 3, 4, 5^2, 7, 8, 30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.419 = TGAGAGGAGTTGTCGT-1

using 10407 reads

====================================================================================

graph has 4306 edges initially, 146 edges after simplification

total ucounts = 389
nonsolo ucounts = 309[2^28, 3^17, 4^17, 5^16, 6^13, 7^15, 8^8, 9^7, 10^4, 11^7, 12^2, 13^2, 14^5, 15^4, 16^6, 17^3, 18^6, 19^4, 20^7, 21^3, 22^4, 23^2, 24^3, 25^7, 26^2, 27^4, 28^5, 29^6, 30^5, 31^2, 32, 33^5, 34^4, 35^7, 36^3, 37, 38^2, 39^4, 40^3, 41, 44^2, 45^4, 46, 47^3, 48, 49^2, 51, 52, 53^2, 55^3, 56, 60, 62, 63, 64^2, 65^3, 72, 73, 74^3, 75, 76^2, 77, 79, 80, 81, 82, 83, 86, 89, 92, 96, 97, 98, 103^3, 104, 108, 109, 111, 114^4, 121, 127, 149, 1981]
surviving nonsolo ucounts = 1[1981]
ids = [235]

====================================================================================

UMI info for barcode TGAGAGGAGTTGTCGT-1 contig 1 = ACAGGACCAG...
umi GCGAGTTCCT = 1972 reads: +397 validated
umi TATCGGGTGG = 1 reads: -395X +1 -1X invalidated

GOOD CONTIGS

TIG 1[bases=502]
0-53 ==> 21-74 on |591|TRBV2|5'UTR| [len=74] (mis=0)
53-391 ==> 0-338 on |592|TRBV2|L-REGION+V-REGION| [len=347] (mis=0)
391-403 ==> 0-12 on |535|TRBD1|D-REGION| [len=12] (mis=1)
402-450 ==> 0-48 on |537|TRBJ1-2|J-REGION| [len=48] (mis=0)
450-502 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 1 umis using 137 reads
cdr3 = CARGQGANYGYTF at 383, score = 6 + 9
umis assigned: [235, 293]
of which 1 are surviving nonsolos
reads assigned: 1946
start codons at 10, 53, 70, 152, 264, 294, 321, 374, 408
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.420 = TGAGAGGCAAACCCAT-1

using 265 reads

====================================================================================

graph has 173 edges initially, 35 edges after simplification

total ucounts = 15
nonsolo ucounts = 15[2^5, 4^3, 5^2, 13, 35, 39, 51, 95]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.422 = TGAGAGGCAAAGCGGT-1

using 52 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 4[3, 11, 12, 25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.424 = TGAGAGGCAACACCTA-1

using 68 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[68]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.426 = TGAGAGGCAAGAGGCT-1

using 5558 reads

====================================================================================

graph has 912 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 1[5555]
surviving nonsolo ucounts = 1[5555]
ids = [3]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.431 = TGAGAGGCAATCCGAT-1

using 36 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[3, 10, 22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.433 = TGAGAGGCAATGCCAT-1

using 129 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[129]
surviving nonsolo ucounts = 1[129]
ids = [0]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.434 = TGAGAGGCACAAGTAA-1

using 77 reads

====================================================================================

graph has 74 edges initially, 4 edges after simplification

total ucounts = 9
nonsolo ucounts = 7[3, 4^2, 6, 7, 24, 27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.436 = TGAGAGGCACAGCGTC-1

using 38 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[3, 13, 20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.438 = TGAGAGGCACCATGTA-1

using 114 reads

====================================================================================

graph has 171 edges initially, 4 edges after simplification

total ucounts = 3
nonsolo ucounts = 3[2, 55, 57]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.445 = TGAGAGGCACGGTAGA-1

using 44 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 1[41]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.448 = TGAGAGGCAGACGTAG-1

using 554 reads

====================================================================================

graph has 466 edges initially, 16 edges after simplification

total ucounts = 29
nonsolo ucounts = 22[2^2, 3, 6, 7, 9, 11^2, 12, 13, 14, 17, 19, 25, 27, 28, 33, 34, 55, 59, 60, 100]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.449 = TGAGAGGCAGACTCGC-1

using 517 reads

====================================================================================

graph has 254 edges initially, 14 edges after simplification

total ucounts = 28
nonsolo ucounts = 24[2^2, 4^2, 5, 6, 7, 8, 9^4, 10, 12, 14, 17, 19, 22, 32, 38, 42, 69, 75, 89]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.452 = TGAGAGGCAGCCTTGG-1

using 45 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[9, 36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.454 = TGAGAGGCAGGAATGC-1

using 46 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 2[5, 37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.456 = TGAGAGGCAGGTGCCT-1

using 61 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[61]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.457 = TGAGAGGCAGTATGCT-1

using 96 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[96]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.459 = TGAGAGGCATAGGATA-1

using 18 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.460 = TGAGAGGCATCACGTA-1

using 83 reads

====================================================================================

graph has 102 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[2, 79]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.464 = TGAGAGGCATGGAATA-1

using 51 reads

====================================================================================

graph has 78 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[50]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.472 = TGAGAGGGTAAGGGAA-1

using 71 reads

====================================================================================

graph has 76 edges initially, 2 edges after simplification

total ucounts = 15
nonsolo ucounts = 8[2, 4, 6, 7, 9, 10, 13^2]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.473 = TGAGAGGGTACCATCA-1

using 54 reads

====================================================================================

graph has 68 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 5[3, 5, 6, 15, 19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.475 = TGAGAGGGTAGGGTAC-1

using 1484 reads

====================================================================================

graph has 348 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 3[3, 8, 1470]
surviving nonsolo ucounts = 1[1470]
ids = [5]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.476 = TGAGAGGGTATAGTAG-1

using 95 reads

====================================================================================

graph has 72 edges initially, 6 edges after simplification

total ucounts = 8
nonsolo ucounts = 6[4^2, 11, 22, 25, 27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.477 = TGAGAGGGTATATCCG-1

using 116 reads

====================================================================================

graph has 127 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 8[2, 3, 5, 7, 8, 9, 11, 69]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.481 = TGAGAGGGTCATTAGC-1

using 26 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.493 = TGAGAGGGTGTCAATC-1

using 33 reads

====================================================================================

graph has 70 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.496 = TGAGAGGGTTACAGAA-1

using 870 reads

====================================================================================

graph has 328 edges initially, 4 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[4, 28, 837]
surviving nonsolo ucounts = 1[837]
ids = [1]

====================================================================================

UMI info for barcode TGAGAGGGTTACAGAA-1 contig 1 = GGGGACAGTG...
umi ACCAAATTTC = 837 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=497]
51-398 ==> 0-347 on |688|TRBV7-9|L-REGION+V-REGION| [len=347] (mis=0)
397-445 ==> 0-48 on |548|TRBJ2-5|J-REGION| [len=48] (mis=3)
445-497 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 1 umis using 49 reads
cdr3 = CASSLALETQYF at 381, score = 7 + 7
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 825
start codons at 51, 75, 160, 259, 372
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.497 = TGAGAGGGTTCAGGCC-1

using 45 reads

====================================================================================

graph has 52 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 4[8, 9, 11, 16]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.498 = TGAGAGGGTTCCATGA-1

using 50 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 5[3, 5, 10, 16^2]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.506 = TGAGAGGTCAACGCTA-1

using 73 reads

====================================================================================

graph has 68 edges initially, 4 edges after simplification

total ucounts = 7
nonsolo ucounts = 5[2, 3, 13, 22, 31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.507 = TGAGAGGTCACATGCA-1

using 71 reads

====================================================================================

graph has 42 edges initially, 4 edges after simplification

total ucounts = 8
nonsolo ucounts = 4[2, 5, 29, 31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.508 = TGAGAGGTCAGATAAG-1

using 32 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.511 = TGAGAGGTCCAAACTG-1

using 48 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[48]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.514 = TGAGAGGTCCAGGGCT-1

using 1908 reads

====================================================================================

graph has 472 edges initially, 4 edges after simplification

total ucounts = 7
nonsolo ucounts = 4[2, 4, 70, 1829]
surviving nonsolo ucounts = 1[1829]
ids = [0]

====================================================================================

REJECT CONTIGS

TIG 1[bases=475]
0-75 ==> 0-75 on |467|TRAV14/DV4|5'UTR| [len=75] (mis=3)
75-419 ==> 0-344 on |468|TRAV14/DV4|L-REGION+V-REGION| [len=350] (mis=3)
433-475 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 1799
start codons at 75, 165, 208, 229, 267, 295, 307, 399, 414
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.517 = TGAGAGGTCCGCAGTG-1

using 48 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[48]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.521 = TGAGAGGTCCGTTGCT-1

using 46 reads

====================================================================================

graph has 46 edges initially, 4 edges after simplification

total ucounts = 5
nonsolo ucounts = 2[20, 23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.522 = TGAGAGGTCCTACAGA-1

using 86 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[86]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.524 = TGAGAGGTCCTTCAAT-1

using 25 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 3[2, 4, 19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.527 = TGAGAGGTCGAGAACG-1

using 34 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.528 = TGAGAGGTCGCAGGCT-1

using 22 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.529 = TGAGAGGTCGCGGATC-1

using 49 reads

====================================================================================

graph has 36 edges initially, 4 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[23, 26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.530 = TGAGAGGTCGGATGGA-1

using 36 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.531 = TGAGAGGTCGTCGTTC-1

using 84 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[84]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.533 = TGAGAGGTCTCCGGTT-1

using 82 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[82]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.543 = TGAGCATAGAGGGATA-1

using 34 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.547 = TGAGCATAGATAGTCA-1

using 154 reads

====================================================================================

graph has 140 edges initially, 4 edges after simplification

total ucounts = 8
nonsolo ucounts = 7[2^2, 4, 6, 13, 63^2]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.560 = TGAGCATAGGGTATCG-1

using 29228 reads

====================================================================================

graph has 8571 edges initially, 172 edges after simplification

total ucounts = 406
nonsolo ucounts = 297[2^43, 3^27, 4^19, 5^15, 6^18, 7^12, 8^12, 9^11, 10^7, 11^13, 12^11, 13^8, 14^8, 15^3, 16^5, 17^5, 18^6, 19^3, 20^3, 21^4, 22^4, 23^4, 24^3, 26^3, 27^2, 28, 30^4, 31, 32, 33^2, 34^2, 37, 38, 40, 43^2, 44, 46, 48^2, 49, 51, 56, 61, 63, 68, 73, 84, 87, 89, 95, 110, 157, 240, 746, 920, 963, 981, 1193, 1314, 1446, 1494, 1556, 1707, 1752, 1831, 4182, 4849]
surviving nonsolo ucounts = 15[240, 746, 920, 963, 981, 1193, 1314, 1446, 1494, 1556, 1707, 1752, 1831, 4182, 4849]
ids = [17, 151, 61, 49, 129, 4, 326, 372, 276, 328, ...]

====================================================================================

UMI info for barcode TGAGCATAGGGTATCG-1 contig 1 = GGAGTGATCC...
umi AACATAAGCC = 1210 reads: +403 validated
umi AATTGCCGGG = 205 reads: -391 +4 -1XX +2 -3XX +2 invalidated
umi ACTCTTAGGT = 1708 reads: +403 validated
umi AGAATTGCAT = 959 reads: +403 validated
umi AGCCGTACAA = 3836 reads: -382X +1 -1XX +1 -3XX +4 -1XX +2 -1XX +2 -2XX +1 -1XX +1 invalidated
umi AGGAAGCGCC = 926 reads: +403 validated
umi ATGGCAACAC = 1846 reads: +403 validated
umi CCCTTAGGAT = 753 reads: +403 validated
umi CCTGCTGGGG = 4851 reads: -166X +1 -2XX +1 -4XX +1 -2XX +1 -2XX +1 -2XX +220 invalidated
umi TAAACTTTAA = 1496 reads: +403 validated
umi TCTAGTCGCC = 1209 reads: -368 +1 -5XX +1 -2XX +1 -1XX +1 -8XX +2 -8XX +2 -1XX +2 invalidated
umi TCTCATTCTC = 1444 reads: -368X +1 -5XX +1 -2XX +1 -1XX +1 -8XX +2 -8XX +2 -1XX +2 invalidated
umi TTCAGAAAGC = 1456 reads: +403 validated

UMI info for barcode TGAGCATAGGGTATCG-1 contig 2 = GGGGGGGGGG...
umi ATGTCAGGAG = 1700 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=502]
0-47 ==> 0-47 on |674|TRBV7-2|5'UTR| [len=47] (mis=2)
47-394 ==> 0-347 on |675|TRBV7-2|L-REGION+V-REGION| [len=347] (mis=2)
400-450 ==> 0-50 on |538|TRBJ1-3|J-REGION| [len=50] (mis=0)
450-502 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 9 umis using 1136 reads
cdr3 = CASSLVSGSGNTIYF at 377, score = 8 + 7
umis assigned: [4, 17, 46, 49, 54, 61, 78, 151, 165, 276] and 3 others
of which 13 are surviving nonsolos
reads assigned: 21584
start codons at 47, 156
confident = true

TIG 2[bases=512]
82-412 ==> 0-330 on |501|TRAV35|L-REGION+V-REGION| [len=330] (mis=4)
407-470 ==> 0-63 on |444|TRAJ58|J-REGION| [len=63] (mis=4)
470-512 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 1 umis using 129 reads
cdr3 = CAGRETSGSRLTF at 400, score = 6 + 9
umis assigned: [82]
of which 1 are surviving nonsolos
reads assigned: 1678
start codons at 82, 115, 126, 166, 188, 196, 234, 308, 383
confident = true
now this is a cell
paired!

TCCATACCTAGTGATGTAGGCATCTACTTCTGTGCTGGGCGAGAAACCAGTGGCTCTAGGTTGACCTTTGGGGAAGGAACACAGCTCACAGTGAATCCTG <==> CAGCAGGAGGACTCAGCCGTGTATCTCTGTGCCAGCAGCTTAGTATCCGGCTCTGGAAACACCATATATTTTGGAGAGGGAAGTTGGCTCACTGTTGTAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.562 = TGAGCATAGGTAAACT-1

using 30 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.563 = TGAGCATAGGTGCACA-1

using 68 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[67]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.564 = TGAGCATAGTACGACG-1

using 49 reads

====================================================================================

graph has 56 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[2, 47]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.566 = TGAGCATAGTAGTGCG-1

using 19 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.572 = TGAGCATAGTGGTAAT-1

using 2351 reads

====================================================================================

graph has 884 edges initially, 8 edges after simplification

total ucounts = 23
nonsolo ucounts = 16[2^3, 3^3, 4, 5, 6, 7, 8, 9, 14, 61, 335, 1880]
surviving nonsolo ucounts = 2[335, 1880]
ids = [10, 1]

====================================================================================

UMI info for barcode TGAGCATAGTGGTAAT-1 contig 1 = AGCTGTGAGG...
umi GATCCCACCC = 322 reads: +400 validated

UMI info for barcode TGAGCATAGTGGTAAT-1 contig 2 = GGGGGAACTG...
umi AGGCCAGAGC = 1891 reads: +397 validated

GOOD CONTIGS

TIG 1[bases=533]
0-81 ==> 0-81 on |585|TRBV18|5'UTR| [len=81] (mis=0)
81-428 ==> 0-347 on |586|TRBV18|L-REGION+V-REGION| [len=347] (mis=2)
430-481 ==> 0-51 on |544|TRBJ2-2|J-REGION| [len=51] (mis=3)
481-533 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 1 umis using 13 reads
cdr3 = CASSPGIDTGELFF at 411, score = 7 + 9
umis assigned: [10]
of which 1 are surviving nonsolos
reads assigned: 317
start codons at 66, 81, 139, 150, 203, 213, 229, 273, 304, 315
confident = false

TIG 2[bases=489]
40-387 ==> 0-347 on |562|TRBV11-1|L-REGION+V-REGION| [len=347] (mis=2)
388-437 ==> 0-49 on |546|TRBJ2-3|J-REGION| [len=49] (mis=5)
437-489 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 1 umis using 114 reads
cdr3 = CASSLPLRDTQYF at 370, score = 8 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 1853
start codons at 40, 64, 182, 248, 263, 361
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.573 = TGAGCATAGTTAGCGG-1

using 17 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[17]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.579 = TGAGCATCAAATTGCC-1

using 52 reads

====================================================================================

graph has 56 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[52]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.580 = TGAGCATCAAGAAGAG-1

using 24 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.585 = TGAGCATCAATCGAAA-1

using 22 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 1[19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.587 = TGAGCATCAATGGTCT-1

using 35 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.588 = TGAGCATCAATGTTGC-1

using 9345 reads

====================================================================================

graph has 3538 edges initially, 44 edges after simplification

total ucounts = 187
nonsolo ucounts = 140[2^33, 3^18, 4^16, 5^5, 6^11, 7^6, 8^6, 9^2, 10^7, 11^3, 12^4, 13^2, 14^2, 15^2, 16^2, 17, 18^2, 19^2, 20^2, 21, 28, 33, 34, 44, 52, 65, 82, 96, 1031, 1156, 1803, 1821, 2251]
surviving nonsolo ucounts = 5[1031, 1156, 1803, 1821, 2251]
ids = [66, 42, 0, 16, 108]

====================================================================================

UMI info for barcode TGAGCATCAATGTTGC-1 contig 1 = GAGAAGCCTC...
umi ATTCTTTCCA = 1164 reads: +406 validated
umi CCCCGCCACA = 1027 reads: +406 validated

UMI info for barcode TGAGCATCAATGTTGC-1 contig 2 = TGGGGACACT...
umi AAAAACGCTG = 1782 reads: +388 validated
umi GCACCTTTTT = 2277 reads: +388 validated

UMI info for barcode TGAGCATCAATGTTGC-1 contig 3 = ATGTTCACAG...
umi ACTACCTGAA = 1836 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=565]
0-117 ==> 0-117 on |474|TRAV19|5'UTR| [len=117] (mis=1)
117-467 ==> 0-350 on |475|TRAV19|L-REGION+V-REGION| [len=350] (mis=3)
471-523 ==> 7-59 on |448|TRAJ7|J-REGION| [len=59] (mis=0)
523-565 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 2 umis using 65 reads
cdr3 = CALSGPGGNNRLAF at 450, score = 7 + 6
umis assigned: [42, 66]
of which 2 are surviving nonsolos
reads assigned: 2157
start codons at 111, 117, 174, 229, 250, 340, 349
confident = true

TIG 2[bases=444]
14-352 ==> 0-338 on |530|TRAV9-2|L-REGION+V-REGION| [len=338] (mis=3)
350-402 ==> 11-63 on |408|TRAJ23|J-REGION| [len=63] (mis=0)
402-444 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 2 umis using 268 reads
cdr3 = CALRQGGKLIF at 338, score = 9 + 9
umis assigned: [0, 108]
of which 2 are surviving nonsolos
reads assigned: 3975
start codons at 14, 89, 177, 231
confident = true

TIG 3[bases=498]
0-52 ==> 7-59 on |567|TRBV12-4|5'UTR| [len=59] (mis=0)
52-399 ==> 0-347 on |568|TRBV12-4|L-REGION+V-REGION| [len=347] (mis=4)
397-446 ==> 0-49 on |546|TRBJ2-3|J-REGION| [len=49] (mis=3)
446-498 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 1 umis using 130 reads
cdr3 = CASSPTGDTQYF at 382, score = 8 + 8
umis assigned: [16]
of which 1 are surviving nonsolos
reads assigned: 1797
start codons at 0, 52, 110, 151, 174, 223, 226, 275, 286, 313, 320
confident = true
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.590 = TGAGCATCACACTGCG-1

using 73 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[73]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.592 = TGAGCATCACAGTCGC-1

using 172 reads

====================================================================================

graph has 74 edges initially, 4 edges after simplification

total ucounts = 7
nonsolo ucounts = 6[2^2, 7, 10, 68, 82]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.595 = TGAGCATCACCTCGTT-1

using 48 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[48]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.596 = TGAGCATCACGCTTTC-1

using 22 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.600 = TGAGCATCAGACGTAG-1

using 10597 reads

====================================================================================

graph has 9955 edges initially, 264 edges after simplification

total ucounts = 1314
nonsolo ucounts = 937[2^153, 3^110, 4^81, 5^63, 6^54, 7^52, 8^54, 9^41, 10^34, 11^21, 12^30, 13^22, 14^19, 15^24, 16^20, 17^16, 18^10, 19^9, 20^8, 21^14, 22^12, 23^9, 24^7, 25^10, 26^6, 27^3, 28^3, 29^2, 30, 31^3, 32^2, 33, 34^3, 35^3, 38^2, 39, 40^2, 41, 42^2, 43^2, 44, 45, 47^2, 48^2, 49^2, 51^3, 53, 59, 64^2, 65, 67, 68, 69^3, 74, 76, 99, 101, 110, 478]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.602 = TGAGCATCAGCCAGAA-1

using 3082 reads

====================================================================================

graph has 2646 edges initially, 68 edges after simplification

total ucounts = 374
nonsolo ucounts = 245[2^42, 3^28, 4^22, 5^24, 6^13, 7^12, 8^10, 9^9, 10^11, 11^3, 12^10, 13^8, 14^3, 15^2, 16^5, 17^3, 18^4, 19^3, 21, 22^4, 23^2, 24^2, 25, 26, 27, 29^2, 30, 32, 36, 39, 43^2, 44, 51, 53, 55, 63, 66, 67, 72, 87, 97, 100, 109, 129]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.604 = TGAGCATCAGCTATTG-1

using 14 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 1[10]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.610 = TGAGCATCATCAGTAC-1

using 23 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.613 = TGAGCATCATTACCTT-1

using 18 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[17]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.617 = TGAGCATGTACCGAGA-1

using 41 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[11^2, 17]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.631 = TGAGCATGTCGCTTTC-1

using 20 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.639 = TGAGCATGTGCTGTAT-1

using 83 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[83]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.646 = TGAGCATGTTCAACCA-1

using 26 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.647 = TGAGCATGTTCCCTTG-1

using 23 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.648 = TGAGCATGTTCCGGCA-1

using 2425 reads

====================================================================================

graph has 694 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[2, 2422]
surviving nonsolo ucounts = 1[2422]
ids = [0]

====================================================================================

UMI info for barcode TGAGCATGTTCCGGCA-1 contig 1 = GGGCTTCAAA...
umi AAGACATAGC = 2442 reads: +400 validated

GOOD CONTIGS

TIG 1[bases=468]
16-360 ==> 0-344 on |613|TRBV28|L-REGION+V-REGION| [len=344] (mis=3)
368-416 ==> 0-48 on |548|TRBJ2-5|J-REGION| [len=48] (mis=0)
416-468 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 1 umis using 171 reads
cdr3 = CASSLQGGNQETQYF at 343, score = 6 + 7
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 2386
start codons at 16, 74, 138, 151, 158, 166, 221, 224, 232, 334
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.649 = TGAGCATGTTGATTCG-1

using 68 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[68]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.650 = TGAGCATTCAAACCGT-1

using 44 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[44]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.653 = TGAGCATTCAAGGTAA-1

using 686 reads

====================================================================================

graph has 362 edges initially, 10 edges after simplification

total ucounts = 23
nonsolo ucounts = 19[5^2, 6, 12^3, 13, 15, 17^2, 21, 30^2, 45, 75, 80, 81, 103^2]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.654 = TGAGCATTCACATACG-1

using 107 reads

====================================================================================

graph has 104 edges initially, 4 edges after simplification

total ucounts = 9
nonsolo ucounts = 6[4, 9^2, 10, 11, 61]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.655 = TGAGCATTCACCATAG-1

using 57 reads

====================================================================================

graph has 42 edges initially, 4 edges after simplification

total ucounts = 8
nonsolo ucounts = 7[2^3, 4, 10, 11, 25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.656 = TGAGCATTCACTATTC-1

using 86 reads

====================================================================================

graph has 66 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[85]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.657 = TGAGCATTCACTTATC-1

using 561 reads

====================================================================================

graph has 324 edges initially, 16 edges after simplification

total ucounts = 38
nonsolo ucounts = 31[2^5, 4^4, 5^2, 6, 7^3, 9, 10, 12^2, 13, 15^2, 17, 26, 28, 37^2, 38, 48, 55, 119]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.658 = TGAGCATTCAGAGCTT-1

using 201 reads

====================================================================================

graph has 96 edges initially, 6 edges after simplification

total ucounts = 12
nonsolo ucounts = 9[3, 6, 13, 16, 17, 21, 22, 50^2]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.659 = TGAGCATTCAGTCAGT-1

using 66 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[66]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.660 = TGAGCATTCATAGCAC-1

using 136 reads

====================================================================================

graph has 308 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[136]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.661 = TGAGCATTCATCGCTC-1

using 86 reads

====================================================================================

graph has 64 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 7[4^3, 6, 15, 23, 27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.663 = TGAGCATTCCACGTGG-1

using 80 reads

====================================================================================

graph has 54 edges initially, 8 edges after simplification

total ucounts = 9
nonsolo ucounts = 8[2^2, 3, 4, 5, 7, 18, 38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.664 = TGAGCATTCCAGAAGG-1

using 146 reads

====================================================================================

graph has 100 edges initially, 4 edges after simplification

total ucounts = 7
nonsolo ucounts = 5[18, 21, 30, 34, 41]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.665 = TGAGCATTCCAGTAGT-1

using 23 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.668 = TGAGCATTCCCTCTTT-1

using 69 reads

====================================================================================

graph has 62 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 6[2, 3, 8, 11, 15, 28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.672 = TGAGCATTCGAGAGCA-1

using 117 reads

====================================================================================

graph has 104 edges initially, 4 edges after simplification

total ucounts = 6
nonsolo ucounts = 6[2, 6, 15, 17, 20, 57]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.675 = TGAGCATTCGGAAATA-1

using 97 reads

====================================================================================

graph has 68 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[2, 5, 29, 59]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.676 = TGAGCATTCGGACAAG-1

using 24425 reads

====================================================================================

graph has 17667 edges initially, 567 edges after simplification

total ucounts = 1573
nonsolo ucounts = 1262[2^142, 3^122, 4^89, 5^60, 6^57, 7^44, 8^41, 9^38, 10^29, 11^33, 12^22, 13^30, 14^32, 15^20, 16^27, 17^22, 18^18, 19^14, 20^24, 21^29, 22^9, 23^15, 24^18, 25^16, 26^13, 27^12, 28^18, 29^13, 30^12, 31^5, 32^8, 33^7, 34^8, 35^4, 36^4, 37^9, 38^6, 39^7, 40^5, 41^4, 42^6, 43^7, 44^7, 45^4, 46^4, 47^2, 48^6, 49^6, 50^7, 51^3, 52^4, 53^5, 54^4, 55^8, 56^7, 57^3, 58^2, 59^3, 60^5, 61^5, 62^6, 63^3, 64^4, 65^4, 66^2, 67^2, 68, 69^6, 70, 71^3, 72^2, 73^2, 74, 75^2, 76^4, 78^2, 81, 82^4, 83, 84, 87^3, 89, 90^2, 92, 93^2, 94^2, 96^2, 99^2, 103, 104, 105, 107, 109^2, 110, 115, 117^2, 140]
surviving nonsolo ucounts = 3[6, 27, 82]
ids = [1493, 1458, 1176]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.677 = TGAGCATTCGGCCGAT-1

using 91 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 7[2, 5, 8, 9, 17, 20, 27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.678 = TGAGCATTCGTGGTCG-1

using 1807 reads

====================================================================================

graph has 705 edges initially, 8 edges after simplification

total ucounts = 31
nonsolo ucounts = 21[2^4, 4, 5^2, 6, 7, 10, 11, 12, 14, 15, 18, 19, 23, 45, 55, 67, 1473]
surviving nonsolo ucounts = 1[1473]
ids = [20]

====================================================================================

UMI info for barcode TGAGCATTCGTGGTCG-1 contig 1 = CTCACAGTGA...
umi TAGTTCATCC = 1486 reads: +406 validated

GOOD CONTIGS

TIG 1[bases=508]
0-50 ==> 0-50 on |678|TRBV7-3|5'UTR| [len=50] (mis=0)
50-397 ==> 0-347 on |679|TRBV7-3|L-REGION+V-REGION| [len=347] (mis=5)
407-456 ==> 0-49 on |546|TRBJ2-3|J-REGION| [len=49] (mis=1)
456-508 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 1 umis using 91 reads
cdr3 = CASSPRGRTSADTQYF at 380, score = 8 + 8
umis assigned: [20]
of which 1 are surviving nonsolos
reads assigned: 1452
start codons at 50, 159, 273
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.680 = TGAGCATTCGTTGACA-1

using 93 reads

====================================================================================

graph has 54 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[93]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.683 = TGAGCATTCTCTGAGA-1

using 224 reads

====================================================================================

graph has 152 edges initially, 8 edges after simplification

total ucounts = 12
nonsolo ucounts = 8[2, 12, 15, 23, 25, 27, 51, 65]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.684 = TGAGCATTCTGAGGGA-1

using 115 reads

====================================================================================

graph has 62 edges initially, 4 edges after simplification

total ucounts = 8
nonsolo ucounts = 7[2^2, 3^2, 10, 15, 79]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.685 = TGAGCATTCTGGTTCC-1

using 121 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[121]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.686 = TGAGCATTCTGTCTAT-1

using 21 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.687 = TGAGCATTCTTCAACT-1

using 101 reads

====================================================================================

graph has 108 edges initially, 2 edges after simplification

total ucounts = 14
nonsolo ucounts = 11[2^2, 3^2, 4, 5, 6, 8, 12, 20, 33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.689 = TGAGCCGAGAAACCGC-1

using 104 reads

====================================================================================

graph has 120 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 8[2^2, 3, 4^2, 6^2, 75]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.691 = TGAGCCGAGAACTCGG-1

using 81 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[81]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.692 = TGAGCCGAGAAGATTC-1

using 87 reads

====================================================================================

graph has 98 edges initially, 2 edges after simplification

total ucounts = 14
nonsolo ucounts = 11[2^2, 3^2, 5, 8, 9, 10^2, 16^2]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.693 = TGAGCCGAGACAGAGA-1

using 110 reads

====================================================================================

graph has 154 edges initially, 2 edges after simplification

total ucounts = 17
nonsolo ucounts = 9[2^2, 3, 4, 8, 11, 13, 18, 41]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.694 = TGAGCCGAGACCTTTG-1

using 46 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 4[3^2, 12, 27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.696 = TGAGCCGAGAGACTTA-1

using 94 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[94]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.697 = TGAGCCGAGAGTAATC-1

using 113 reads

====================================================================================

graph has 160 edges initially, 4 edges after simplification

total ucounts = 29
nonsolo ucounts = 18[2^4, 3^3, 4^2, 5^3, 7, 9^3, 12, 16]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.700 = TGAGCCGAGATATGCA-1

using 106 reads

====================================================================================

graph has 88 edges initially, 4 edges after simplification

total ucounts = 13
nonsolo ucounts = 8[2, 3, 7, 9^2, 12, 14, 45]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.703 = TGAGCCGAGATGGCGT-1

using 25 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 4[2, 3, 6, 11]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.707 = TGAGCCGAGCCAGTTT-1

using 51 reads

====================================================================================

graph has 78 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 5[2, 3, 8, 17^2]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.710 = TGAGCCGAGCGAGAAA-1

using 110 reads

====================================================================================

graph has 126 edges initially, 2 edges after simplification

total ucounts = 14
nonsolo ucounts = 8[2^2, 3, 11^2, 13, 27, 35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.711 = TGAGCCGAGCGATATA-1

using 77 reads

====================================================================================

graph has 76 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 7[2^2, 4, 6, 16, 18, 28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.714 = TGAGCCGAGCTCCTTC-1

using 82 reads

====================================================================================

graph has 87 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 7[4^2, 6^2, 9, 14, 37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.715 = TGAGCCGAGGAGCGTT-1

using 141 reads

====================================================================================

graph has 58 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[141]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.717 = TGAGCCGAGGATCGCA-1

using 74 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[74]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.724 = TGAGCCGAGGGATACC-1

using 51 reads

====================================================================================

graph has 92 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 7[2^2, 4, 5, 10, 13^2]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.725 = TGAGCCGAGGGATGGG-1

using 26092 reads

====================================================================================

graph has 8884 edges initially, 138 edges after simplification

total ucounts = 881
nonsolo ucounts = 659[2^94, 3^67, 4^62, 5^55, 6^37, 7^33, 8^28, 9^32, 10^25, 11^18, 12^17, 13^26, 14^18, 15^21, 16^15, 17^18, 18^9, 19^4, 20^8, 21^7, 22^8, 23^3, 24^3, 25^3, 26^2, 27, 28^4, 29^3, 30^3, 31, 32, 33^2, 34^2, 35, 36^2, 37, 39, 41, 43, 44, 46, 49, 51, 53, 56^2, 57, 58, 62, 65, 66, 73, 79, 88, 142, 317, 369, 447, 2841, 7440, 7662]
surviving nonsolo ucounts = 6[317, 369, 447, 2841, 7440, 7662]
ids = [321, 127, 397, 133, 345, 829]

====================================================================================

REJECT CONTIGS

TIG 1[bases=321]
5-219 ==> 3012-3226 on segment before TRBJ2-1 exon 1 [len=3226] (mis=0)
219-269 ==> 0-50 on |543|TRBJ2-1|J-REGION| [len=50] (mis=0)
269-321 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
umis assigned: [321, 345]
of which 2 are surviving nonsolos
reads assigned: 7607
start codons at 78, 227
confident = false
did not find CDR3

TIG 2[bases=313]
5-60 ==> 49-104 on segment before TRBJ2-3 exon 1 [len=104] (mis=0)
60-109 ==> 0-49 on |546|TRBJ2-3|J-REGION| [len=49] (mis=0)
109-211 ==> 0-102 on segment before TRBJ2-4 exon 1 [len=102] (mis=0)
261-313 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
umis assigned: [133, 397, 829, 854]
of which 3 are surviving nonsolos
reads assigned: 10730
start codons at 
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.726 = TGAGCCGAGGGCTCTC-1

using 85 reads

====================================================================================

graph has 122 edges initially, 6 edges after simplification

total ucounts = 10
nonsolo ucounts = 9[2, 3, 5^2, 11^3, 16, 20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.738 = TGAGCCGAGTGGGTTG-1

using 113 reads

====================================================================================

graph has 89 edges initially, 4 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[53, 60]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.739 = TGAGCCGAGTGTCCCG-1

using 66 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[66]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.740 = TGAGCCGAGTGTTAGA-1

using 20 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[2^2, 7^2]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.744 = TGAGCCGCAAACTGTC-1

using 42 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[42]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.747 = TGAGCCGCAACACCTA-1

using 70 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[70]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.750 = TGAGCCGCAAGACGTG-1

using 105 reads

====================================================================================

graph has 104 edges initially, 4 edges after simplification

total ucounts = 9
nonsolo ucounts = 8[3, 4, 8, 10, 11^2, 24, 33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.753 = TGAGCCGCAATAGAGT-1

using 90 reads

====================================================================================

graph has 98 edges initially, 2 edges after simplification

total ucounts = 13
nonsolo ucounts = 8[2, 3^2, 6, 7, 12, 22, 30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.755 = TGAGCCGCAATGAATG-1

using 498 reads

====================================================================================

graph has 382 edges initially, 18 edges after simplification

total ucounts = 34
nonsolo ucounts = 26[2^4, 3^3, 4, 5^4, 7, 8, 10, 11, 12, 13, 14, 25^2, 44, 65, 69, 73^2]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.761 = TGAGCCGCACCGAATT-1

using 23 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.764 = TGAGCCGCACGGATAG-1

using 69 reads

====================================================================================

graph has 64 edges initially, 8 edges after simplification

total ucounts = 8
nonsolo ucounts = 8[2^2, 3, 6, 8, 10, 12, 26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.769 = TGAGCCGCACTTAACG-1

using 21 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.770 = TGAGCCGCAGCATGAG-1

using 65 reads

====================================================================================

graph has 102 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 8[2^3, 3, 6, 11^2, 27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.788 = TGAGCCGCATCGTCGG-1

using 123 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[122]
surviving nonsolo ucounts = 1[122]
ids = [0]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.789 = TGAGCCGCATCTATGG-1

using 394 reads

====================================================================================

graph has 330 edges initially, 10 edges after simplification

total ucounts = 103
nonsolo ucounts = 55[2^19, 3^10, 4^5, 5^4, 6^4, 7^4, 8^2, 9, 10^2, 15, 18, 24, 84]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.791 = TGAGCCGCATGATCCA-1

using 467 reads

====================================================================================

graph has 238 edges initially, 4 edges after simplification

total ucounts = 24
nonsolo ucounts = 21[2^2, 4, 5, 7, 9, 10, 13, 15, 18, 21, 24^2, 25, 26, 28, 33, 34, 37, 55, 72]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.795 = TGAGCCGCATTCCTGC-1

using 104 reads

====================================================================================

graph has 48 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[104]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.796 = TGAGCCGCATTCGACA-1

using 4500 reads

====================================================================================

graph has 3975 edges initially, 78 edges after simplification

total ucounts = 615
nonsolo ucounts = 454[2^68, 3^43, 4^44, 5^32, 6^28, 7^24, 8^22, 9^29, 10^20, 11^13, 12^16, 13^16, 14^12, 15^13, 16^9, 17^5, 18^7, 19^9, 20^11, 21^2, 22^2, 23, 24, 25, 26^2, 27^3, 29^2, 32^2, 33^2, 34^2, 35^3, 36, 37, 38, 40, 41, 46, 47, 49, 58, 69]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.800 = TGAGCCGGTAAGTGTA-1

using 111 reads

====================================================================================

graph has 104 edges initially, 4 edges after simplification

total ucounts = 7
nonsolo ucounts = 5[3, 4, 5, 32, 65]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.801 = TGAGCCGGTACAGCAG-1

using 47 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[47]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.804 = TGAGCCGGTACGCACC-1

using 139 reads

====================================================================================

graph has 154 edges initially, 24 edges after simplification

total ucounts = 29
nonsolo ucounts = 22[2^6, 3^5, 5^2, 6, 7^2, 8, 9, 11^2, 17, 19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.806 = TGAGCCGGTACTTAGC-1

using 332 reads

====================================================================================

graph has 202 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[332]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.810 = TGAGCCGGTATATGAG-1

using 84 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[84]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.816 = TGAGCCGGTCGCGGTT-1

using 26 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.818 = TGAGCCGGTCGCTTTC-1

using 39 reads

====================================================================================

graph has 6 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.819 = TGAGCCGGTCGGCATC-1

using 101 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[101]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.820 = TGAGCCGGTCTAACGT-1

using 48 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 4[2, 4, 17, 24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.826 = TGAGCCGGTGCAACGA-1

using 86 reads

====================================================================================

graph has 118 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 6[2, 3, 4, 6, 10, 58]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.828 = TGAGCCGGTGGACGAT-1

using 66 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 4[6, 12^2, 35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.833 = TGAGCCGGTTACCAGT-1

using 117 reads

====================================================================================

graph has 102 edges initially, 6 edges after simplification

total ucounts = 9
nonsolo ucounts = 7[2, 4, 6, 16, 22, 29, 36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.836 = TGAGCCGGTTATCGGT-1

using 75 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[75]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.837 = TGAGCCGGTTCATGGT-1

using 44 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[44]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.839 = TGAGCCGGTTCCCTTG-1

using 113 reads

====================================================================================

graph has 154 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[113]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.840 = TGAGCCGGTTCGCTAA-1

using 159 reads

====================================================================================

graph has 162 edges initially, 4 edges after simplification

total ucounts = 17
nonsolo ucounts = 13[2, 4^2, 5^2, 6, 9, 10, 11^2, 14, 16, 58]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.841 = TGAGCCGGTTCGGCAC-1

using 48 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[48]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.842 = TGAGCCGGTTCGGGCT-1

using 6752 reads

====================================================================================

graph has 2577 edges initially, 46 edges after simplification

total ucounts = 616
nonsolo ucounts = 477[2^70, 3^43, 4^35, 5^30, 6^22, 7^20, 8^24, 9^12, 10^19, 11^13, 12^17, 13^15, 14^7, 15^9, 16^4, 17^11, 18^2, 19^5, 20^9, 21^5, 22^6, 23^6, 24^8, 25^8, 26^6, 27^3, 28^5, 29^4, 30^4, 31^8, 32^2, 33^3, 34^5, 35, 36^3, 37^5, 38^2, 39^2, 40, 41^2, 42^2, 43^2, 44^2, 45^3, 48, 49, 51^2, 54, 57, 63, 82, 106, 117, 150, 169]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.846 = TGAGCCGGTTGCGCAC-1

using 108 reads

====================================================================================

graph has 106 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 7[2^2, 6, 7, 8, 24, 56]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.848 = TGAGCCGGTTTCGCTC-1

using 33 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 5[3^2, 5, 6, 14]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.852 = TGAGCCGTCAACACGT-1

using 107 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[107]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.854 = TGAGCCGTCACAGGCC-1

using 62 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[62]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.855 = TGAGCCGTCACCAGGC-1

using 59 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[58]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.860 = TGAGCCGTCAGCCTAA-1

using 18 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.862 = TGAGCCGTCAGTGTTG-1

using 125 reads

====================================================================================

graph has 168 edges initially, 2 edges after simplification

total ucounts = 19
nonsolo ucounts = 15[2^6, 4, 5^2, 6, 8, 9, 12, 19, 41]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.863 = TGAGCCGTCATCGATG-1

using 56 reads

====================================================================================

graph has 52 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 1[54]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.865 = TGAGCCGTCATGTCTT-1

using 11 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[11]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.870 = TGAGCCGTCCACGAAT-1

using 70 reads

====================================================================================

graph has 48 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[70]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.875 = TGAGCCGTCCCATTAT-1

using 47115 reads

====================================================================================

graph has 15993 edges initially, 372 edges after simplification

total ucounts = 1170
nonsolo ucounts = 980[2^71, 3^51, 4^38, 5^41, 6^31, 7^38, 8^32, 9^22, 10^29, 11^23, 12^26, 13^28, 14^27, 15^25, 16^23, 17^25, 18^27, 19^14, 20^12, 21^12, 22^13, 23^9, 24^15, 25^10, 26^6, 27^6, 28^7, 29^10, 30^7, 31^10, 32^3, 33^8, 34^7, 35^12, 36^7, 37^7, 38^10, 39^10, 40^10, 41^9, 42^6, 43^8, 44^5, 45^2, 46^7, 47^5, 48^7, 49^4, 50^6, 51^3, 52^5, 53^2, 54^8, 55^2, 56^5, 57^9, 58^2, 59^3, 60^2, 61^4, 62^3, 63^2, 64, 65^5, 66^2, 67^6, 68^2, 69^6, 70^2, 71^4, 72^2, 74^4, 75^2, 76, 77^3, 78^5, 80^4, 81, 82, 83^3, 84^2, 85^2, 86^2, 87^3, 88, 89, 90^6, 91^2, 92, 95, 96^2, 97^2, 98, 99^3, 101^2, 102^2, 103, 108, 111, 113, 117, 118, 122, 123, 125, 129, 145, 297, 396, 691, 1046, 1188, 1635, 1641, 1691, 1866, 3655, 8365]
surviving nonsolo ucounts = 11[297, 396, 691, 1046, 1188, 1635, 1641, 1691, 1866, 3655, 8365]
ids = [149, 245, 668, 381, 748, 122, 1131, 310, 394, 111, ...]

====================================================================================

UMI info for barcode TGAGCCGTCCCATTAT-1 contig 1 = CCAGGAGGAC...
umi AGCGGCGGCT = 3425 reads: -363 +1 -2XX +3 -2XX +3 -1XX +2 -1XX +1 -2XX +3 -3XX +11 -1XX +4 invalidated
umi AGTCTCCTTT = 1641 reads: +403 validated
umi ATCCTCAGCT = 258 reads: -368X +2 -2X +2 -1XX +2 -3XX +4 -1XX +1 -2XX +4 -4XX +2 -1XX +2 -1XX +1 invalidated
umi CATCACGATC = 401 reads: +403 validated
umi CCAGCTTGTA = 1698 reads: +403 validated
umi CCCTCTTGGA = 37 reads: -395 +1 -1X +1 -1XX +2 -1XX +1 invalidated
umi CCGCCCTTAC = 1883 reads: +403 validated
umi CTTCTGCGGC = 700 reads: +403 validated
umi GCGTAGCCAT = 1185 reads: +403 validated
umi TTGGTTCGGT = 1646 reads: +402 -1XX invalidated

GOOD CONTIGS

TIG 1[bases=508]
0-53 ==> 256-309 on |630|TRBV5-1|5'UTR| [len=309] (mis=0)
53-396 ==> 0-343 on |631|TRBV5-1|L-REGION+V-REGION| [len=343] (mis=2)
391-407 ==> 0-16 on |730|TRBD2|D-REGION| [len=16] (mis=3)
405-456 ==> 0-51 on |544|TRBJ2-2|J-REGION| [len=51] (mis=1)
456-508 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 6 umis using 476 reads
cdr3 = CASSLLAGGNTGELFF at 380, score = 9 + 9
umis assigned: [111, 122, 149, 245, 310, 381, 394, 668, 748, 1131]
of which 10 are surviving nonsolos
reads assigned: 12698
start codons at 53, 335, 339
confident = true

REJECT CONTIGS

TIG 1[bases=322]
3-214 ==> 116-327 on segment before TRAJ18 exon 1 [len=327] (mis=0)
214-280 ==> 0-66 on |402|TRAJ18|J-REGION| [len=66] (mis=0)
280-322 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
umis assigned: [652, 939]
of which 1 are surviving nonsolos
reads assigned: 8298
start codons at 106, 119, 167, 191
confident = false
did not find CDR3
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.881 = TGAGCCGTCCTCAATT-1

using 42 reads

====================================================================================

graph has 78 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 6[2^2, 4, 9^2, 14]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.882 = TGAGCCGTCCTTCAAT-1

using 60 reads

====================================================================================

graph has 100 edges initially, 6 edges after simplification

total ucounts = 10
nonsolo ucounts = 8[2, 3, 4, 7^2, 9, 11, 15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.890 = TGAGCCGTCTCGAGTA-1

using 62 reads

====================================================================================

graph has 74 edges initially, 2 edges after simplification

total ucounts = 13
nonsolo ucounts = 8[4^4, 6^2, 8, 21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.891 = TGAGCCGTCTCGGACG-1

using 82 reads

====================================================================================

graph has 120 edges initially, 2 edges after simplification

total ucounts = 13
nonsolo ucounts = 10[2^2, 4^2, 5^2, 6, 8, 21, 22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.894 = TGAGCCGTCTGTCAAG-1

using 47 reads

====================================================================================

graph has 72 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 6[2, 3, 7, 8, 9, 14]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.895 = TGAGCCGTCTTCAACT-1

using 59 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[59]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.899 = TGAGGGAAGAATAGGG-1

using 69 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[4, 65]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.901 = TGAGGGAAGACCGGAT-1

using 55 reads

====================================================================================

graph has 72 edges initially, 2 edges after simplification

total ucounts = 13
nonsolo ucounts = 8[2^2, 3, 4^2, 5, 6, 24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.903 = TGAGGGAAGACTAAGT-1

using 19 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.907 = TGAGGGAAGAGTAATC-1

using 31 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.912 = TGAGGGAAGCCAGAAC-1

using 70 reads

====================================================================================

graph has 72 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 3[2, 4, 59]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.913 = TGAGGGAAGCCAGTTT-1

using 45 reads

====================================================================================

graph has 64 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 8[2, 3^3, 4^2, 5, 20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.916 = TGAGGGAAGCGGCTTC-1

using 18 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.922 = TGAGGGAAGGATATAC-1

using 48 reads

====================================================================================

graph has 56 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 5[2, 4, 6, 17^2]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.930 = TGAGGGAAGGTGCTAG-1

using 38 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.931 = TGAGGGAAGGTGCTTT-1

using 50232 reads

====================================================================================

graph has 26190 edges initially, 920 edges after simplification

total ucounts = 1857
nonsolo ucounts = 1593[2^131, 3^78, 4^73, 5^60, 6^58, 7^56, 8^40, 9^42, 10^42, 11^43, 12^41, 13^27, 14^26, 15^16, 16^32, 17^30, 18^18, 19^20, 20^20, 21^15, 22^15, 23^13, 24^14, 25^17, 26^19, 27^19, 28^18, 29^16, 30^11, 31^13, 32^11, 33^10, 34^12, 35^15, 36^12, 37^13, 38^7, 39^13, 40^9, 41^14, 42^14, 43^11, 44^13, 45^8, 46^13, 47^9, 48^11, 49^8, 50^9, 51^12, 52^10, 53^7, 54^10, 55^9, 56^13, 57^12, 58^8, 59^8, 60^13, 61^9, 62^10, 63^6, 64^11, 65^7, 66^4, 67^5, 68^8, 69^5, 70^6, 71^6, 72^2, 73^9, 74^7, 75^3, 76^7, 77^6, 78^9, 79^8, 80^4, 81^5, 82^4, 83^9, 84^5, 85^7, 86^5, 87^3, 88^3, 89^2, 90^2, 91^7, 92^4, 93, 94^3, 95^2, 96^4, 97^6, 98^3, 99^3, 100, 101, 102, 103^4, 104^2, 106, 107, 108^2, 109, 110^2, 111^4, 112, 113^2, 114^3, 115, 117, 118, 119, 120, 121, 122^2, 123, 124, 125, 127, 130, 131, 135, 140, 160, 361, 682, 2114]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.933 = TGAGGGAAGTACGCGA-1

using 67 reads

====================================================================================

graph has 66 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 6[2^2, 4, 11, 12, 35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.935 = TGAGGGAAGTATTGGA-1

using 28 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 5[3^2, 4, 8, 9]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.941 = TGAGGGAAGTGAAGAG-1

using 101 reads

====================================================================================

graph has 62 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[101]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.943 = TGAGGGAAGTGTTGAA-1

using 479 reads

====================================================================================

graph has 331 edges initially, 16 edges after simplification

total ucounts = 35
nonsolo ucounts = 30[2^4, 3^2, 4^2, 5, 6, 7, 8, 10, 12^2, 14^2, 16^2, 17, 18, 19, 23^2, 30, 34, 35, 41, 45, 47]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.946 = TGAGGGAAGTTCGCAT-1

using 239 reads

====================================================================================

graph has 178 edges initially, 6 edges after simplification

total ucounts = 9
nonsolo ucounts = 7[6, 8, 15, 16, 33, 79, 80]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.961 = TGAGGGACACCAGCAC-1

using 80 reads

====================================================================================

graph has 62 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[80]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.963 = TGAGGGACACCCATGG-1

using 38 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 7[4^3, 5, 6^3]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.965 = TGAGGGACACCGTTGG-1

using 120 reads

====================================================================================

graph has 64 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[120]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.966 = TGAGGGACACCTCGGA-1

using 66 reads

====================================================================================

graph has 54 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 5[2, 3, 4, 11, 46]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.967 = TGAGGGACACGAAAGC-1

using 122 reads

====================================================================================

graph has 86 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 6[3, 5^2, 6, 11, 89]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.971 = TGAGGGACAGACGCAA-1

using 37 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.973 = TGAGGGACAGCGAACA-1

using 62 reads

====================================================================================

graph has 56 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[9, 51]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.976 = TGAGGGACAGGATCGA-1

using 62 reads

====================================================================================

graph has 170 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[62]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.981 = TGAGGGACAGTTTACG-1

using 82 reads

====================================================================================

graph has 84 edges initially, 2 edges after simplification

total ucounts = 13
nonsolo ucounts = 9[2^2, 3, 4^2, 5^2, 9, 44]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.983 = TGAGGGACATATACCG-1

using 2592 reads

====================================================================================

graph has 876 edges initially, 6 edges after simplification

total ucounts = 22
nonsolo ucounts = 11[3^2, 4, 5^2, 7, 9, 10, 18, 45, 2472]
surviving nonsolo ucounts = 1[2472]
ids = [12]

====================================================================================

UMI info for barcode TGAGGGACATATACCG-1 contig 1 = ATTCTTTCTT...
umi GCCACCATTA = 2470 reads: +400 validated

GOOD CONTIGS

TIG 1[bases=472]
0-20 ==> 0-20 on |612|TRBV28|5'UTR| [len=20] (mis=0)
20-364 ==> 0-344 on |613|TRBV28|L-REGION+V-REGION| [len=344] (mis=5)
376-420 ==> 6-50 on |543|TRBJ2-1|J-REGION| [len=50] (mis=0)
420-472 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 1 umis using 165 reads
cdr3 = CASSHYSGRTNEQFF at 347, score = 6 + 8
umis assigned: [12]
of which 1 are surviving nonsolos
reads assigned: 2439
start codons at 20, 78, 142, 155, 162, 170, 225, 228, 236, 338, 378
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.985 = TGAGGGACATCTGGTA-1

using 108 reads

====================================================================================

graph has 116 edges initially, 6 edges after simplification

total ucounts = 12
nonsolo ucounts = 9[2, 3, 5, 7, 11^2, 18, 20, 28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.987 = TGAGGGACATGCCACG-1

using 62 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[62]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.990 = TGAGGGACATTGGGCC-1

using 34 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.993 = TGAGGGAGTACCGCTG-1

using 2238 reads

====================================================================================

graph has 618 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 4[2, 3, 7, 2222]
surviving nonsolo ucounts = 1[2222]
ids = [7]

====================================================================================

UMI info for barcode TGAGGGAGTACCGCTG-1 contig 1 = GGGAGCCATG...
umi TCTAGGGCGT = 2235 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=453]
7-351 ==> 0-344 on |613|TRBV28|L-REGION+V-REGION| [len=344] (mis=3)
361-401 ==> 10-50 on |543|TRBJ2-1|J-REGION| [len=50] (mis=0)
401-453 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 1 umis using 174 reads
cdr3 = CASSLGTSGEQFF at 334, score = 6 + 8
umis assigned: [7]
of which 1 are surviving nonsolos
reads assigned: 2203
start codons at 7, 65, 129, 142, 149, 157, 212, 215, 223, 325
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.995 = TGAGGGAGTAGAAGGA-1

using 32 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.999 = TGAGGGAGTAGTACCT-1

using 105 reads

====================================================================================

graph has 74 edges initially, 2 edges after simplification

total ucounts = 12
nonsolo ucounts = 7[2^2, 3, 5, 21, 28, 39]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1003 = TGAGGGAGTCATATGC-1

using 86 reads

====================================================================================

graph has 118 edges initially, 2 edges after simplification

total ucounts = 13
nonsolo ucounts = 10[2^2, 4, 5, 6, 7^2, 8, 17, 25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1005 = TGAGGGAGTCGAAAGC-1

using 71 reads

====================================================================================

graph has 48 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[71]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1007 = TGAGGGAGTCGACTAT-1

using 2443 reads

====================================================================================

graph has 698 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 2[2, 2436]
surviving nonsolo ucounts = 1[2436]
ids = [4]

====================================================================================

UMI info for barcode TGAGGGAGTCGACTAT-1 contig 1 = CACAACTCTC...
umi AATCAATCTG = 2438 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=483]
0-43 ==> 35-78 on |597|TRBV20-1|5'UTR| [len=78] (mis=0)
43-378 ==> 0-335 on |598|TRBV20-1|L-REGION+V-REGION| [len=335] (mis=3)
374-386 ==> 0-12 on |535|TRBD1|D-REGION| [len=12] (mis=3)
384-431 ==> 0-47 on |550|TRBJ2-7|J-REGION| [len=47] (mis=2)
431-483 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 1 umis using 147 reads
cdr3 = CSAPDRGPYEQYF at 364, score = 7 + 8
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 2402
start codons at 43, 181, 217, 223, 236, 296
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1014 = TGAGGGAGTGATAAGT-1

using 103 reads

====================================================================================

graph has 62 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[103]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1020 = TGAGGGAGTTACGACT-1

using 136 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[136]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1022 = TGAGGGAGTTATCGGT-1

using 29 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 5[2, 3, 4, 5, 13]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1033 = TGAGGGATCACCACCT-1

using 67 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[66]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1034 = TGAGGGATCACCATAG-1

using 109 reads

====================================================================================

graph has 151 edges initially, 4 edges after simplification

total ucounts = 11
nonsolo ucounts = 6[2, 3, 5, 9, 41, 44]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1036 = TGAGGGATCATGCATG-1

using 34 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 6[2, 4^2, 7^3]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1040 = TGAGGGATCCCACTTG-1

using 54 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[7, 9, 36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1041 = TGAGGGATCCGATATG-1

using 69 reads

====================================================================================

graph has 112 edges initially, 4 edges after simplification

total ucounts = 11
nonsolo ucounts = 9[2^5, 3, 10, 11, 33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1042 = TGAGGGATCCGTAGGC-1

using 122 reads

====================================================================================

graph has 84 edges initially, 4 edges after simplification

total ucounts = 8
nonsolo ucounts = 5[3, 7, 8, 33, 68]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1044 = TGAGGGATCCTTAATC-1

using 64 reads

====================================================================================

graph has 80 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 7[2, 3, 4, 7^2, 9, 31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1046 = TGAGGGATCGCCATAA-1

using 56 reads

====================================================================================

graph has 54 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 6[2, 3, 7^2, 12, 23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1052 = TGAGGGATCGTCACGG-1

using 88 reads

====================================================================================

graph has 64 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 9[2^3, 3, 4, 5, 7^2, 54]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1060 = TGAGGGATCTTTACAC-1

using 139 reads

====================================================================================

graph has 179 edges initially, 11 edges after simplification

total ucounts = 28
nonsolo ucounts = 15[2^2, 3, 4^3, 5, 7, 8, 9, 11^2, 17, 18, 21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1061 = TGATTTCAGACCACGA-1

using 6677 reads

====================================================================================

graph has 1031 edges initially, 4 edges after simplification

total ucounts = 11
nonsolo ucounts = 7[2^3, 3, 5, 15, 6644]
surviving nonsolo ucounts = 1[6644]
ids = [6]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1062 = TGATTTCAGAGAACAG-1

using 66 reads

====================================================================================

graph has 64 edges initially, 6 edges after simplification

total ucounts = 11
nonsolo ucounts = 8[4^2, 6^2, 9, 10^2, 14]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1063 = TGATTTCAGAGCAATT-1

using 150 reads

====================================================================================

graph has 108 edges initially, 4 edges after simplification

total ucounts = 12
nonsolo ucounts = 10[2^3, 3^3, 12, 13, 21, 87]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1066 = TGATTTCAGATCCCAT-1

using 77 reads

====================================================================================

graph has 82 edges initially, 4 edges after simplification

total ucounts = 15
nonsolo ucounts = 10[4^2, 6^2, 7^2, 8^2, 9, 13]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1068 = TGATTTCAGATGCCAG-1

using 52 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 4[2, 6, 7, 36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1070 = TGATTTCAGCCAACAG-1

using 312 reads

====================================================================================

graph has 243 edges initially, 12 edges after simplification

total ucounts = 17
nonsolo ucounts = 15[2^4, 4^2, 5, 7, 12, 13, 15, 34^2, 45, 129]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1074 = TGATTTCAGCTACCGC-1

using 33 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[2^2, 13, 14]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1076 = TGATTTCAGCTAGTTC-1

using 9222 reads

====================================================================================

graph has 7601 edges initially, 335 edges after simplification

total ucounts = 1012
nonsolo ucounts = 715[2^107, 3^68, 4^58, 5^48, 6^39, 7^37, 8^31, 9^35, 10^16, 11^20, 12^20, 13^13, 14^23, 15^4, 16^15, 17^16, 18^12, 19^12, 20^5, 21^10, 22^7, 23^5, 24^5, 25^11, 26^12, 27^5, 28^5, 29^9, 30, 31^2, 32^2, 33^9, 34^4, 35^6, 37^2, 38^3, 39^2, 40, 41^2, 42^4, 43, 44^3, 45, 49, 50, 51^2, 52^4, 53, 54^4, 55, 56^3, 57, 67, 72, 80, 87, 92, 99]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1077 = TGATTTCAGCTGAACG-1

using 50 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 7[3^2, 4, 6, 10, 11, 12]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1082 = TGATTTCAGGGAAACA-1

using 51 reads

====================================================================================

graph has 100 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[50]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1083 = TGATTTCAGGGATACC-1

using 36 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1084 = TGATTTCAGGGCTTGA-1

using 33 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 4[2, 5, 7, 16]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1086 = TGATTTCAGGTAGCTG-1

using 27 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1087 = TGATTTCAGGTCATCT-1

using 45 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[45]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1090 = TGATTTCAGGTTCCTA-1

using 81 reads

====================================================================================

graph has 66 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 7[2^3, 4, 12, 14, 45]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1091 = TGATTTCAGTAATCCC-1

using 90 reads

====================================================================================

graph has 99 edges initially, 2 edges after simplification

total ucounts = 12
nonsolo ucounts = 10[2, 3^3, 4, 5, 6, 7, 15, 40]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1092 = TGATTTCAGTATCGAA-1

using 71 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[70]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1094 = TGATTTCAGTCCTCCT-1

using 4606 reads

====================================================================================

graph has 3221 edges initially, 88 edges after simplification

total ucounts = 387
nonsolo ucounts = 256[2^49, 3^29, 4^24, 5^15, 6^13, 7^11, 8^11, 9^10, 10^7, 11^7, 12^5, 13^9, 14^3, 15^8, 16^7, 17^2, 18^2, 19^6, 20, 21^2, 22^4, 23, 24^4, 25^2, 26, 28, 29^2, 30, 31, 32, 33, 34^2, 35^2, 36, 38^3, 40, 41^2, 51, 70, 92, 199, 1625]
surviving nonsolo ucounts = 4[35, 92, 199, 1625]
ids = [201, 37, 204, 203]

====================================================================================

UMI info for barcode TGATTTCAGTCCTCCT-1 contig 1 = GGAAATGTGA...
umi GCCGTGGGTG = 37 reads: +332 -21 +56 non-validated
umi GCCGTGTGTC = 1638 reads: +409 validated
umi GCCGTGTGTG = 204 reads: +409 validated

GOOD CONTIGS

TIG 1[bases=488]
0-27 ==> 32-59 on |576|TRBV14|5'UTR| [len=59] (mis=0)
27-374 ==> 0-347 on |577|TRBV14|L-REGION+V-REGION| [len=347] (mis=0)
386-436 ==> 0-50 on |543|TRBJ2-1|J-REGION| [len=50] (mis=4)
436-488 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 2 umis using 128 reads
cdr3 = CASSQDWGLAGYNEQFF at 357, score = 9 + 8
umis assigned: [201, 203, 204]
of which 3 are surviving nonsolos
reads assigned: 1847
start codons at 4, 27, 149, 169, 198, 250, 261, 394
confident = true
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1096 = TGATTTCAGTGAAGTT-1

using 90 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 5[2, 3, 4, 8, 72]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1098 = TGATTTCAGTGCCAGA-1

using 65 reads

====================================================================================

graph has 52 edges initially, 4 edges after simplification

total ucounts = 10
nonsolo ucounts = 7[2^2, 3, 7, 9, 10, 29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1100 = TGATTTCAGTGTACTC-1

using 133 reads

====================================================================================

graph has 120 edges initially, 4 edges after simplification

total ucounts = 11
nonsolo ucounts = 8[2, 5^2, 7, 10, 12, 17, 72]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1101 = TGATTTCAGTGTCCAT-1

using 7199 reads

====================================================================================

graph has 3623 edges initially, 103 edges after simplification

total ucounts = 327
nonsolo ucounts = 234[2^35, 3^21, 4^20, 5^16, 6^15, 7^11, 8^13, 9^8, 10^6, 11^7, 12^7, 13^5, 14^6, 15^5, 16^10, 17^4, 18^6, 19^4, 20^2, 21^2, 22, 23^3, 24^2, 25^3, 26^3, 31, 32^2, 33, 34, 38, 40, 42, 43, 45, 54, 63, 121, 123, 142, 508, 581, 1429, 1848]
surviving nonsolo ucounts = 5[121, 508, 581, 1429, 1848]
ids = [179, 116, 294, 106, 259]

====================================================================================

UMI info for barcode TGATTTCAGTGTCCAT-1 contig 1 = GCCAGGTTCA...
umi TTCTCGGCAG = 552 reads: +415 validated
umi TTGTGGCGTG = 2 reads: -402X +3 -1XX +1 -7XX +1 invalidated

UMI info for barcode TGATTTCAGTGTCCAT-1 contig 2 = ATGCTCACAG...
umi CGACTGGTCG = 1445 reads: +406 validated
umi CGGAGACGCA = 508 reads: +406 validated
umi GCGTGATCTA = 118 reads: +406 validated
umi TACCGGGTCG = 111 reads: -405X +1 invalidated
umi TCTCATTCAG = 1850 reads: -40 +3 -6XX +357 invalidated

GOOD CONTIGS

TIG 1[bases=532]
0-75 ==> 0-75 on |467|TRAV14/DV4|5'UTR| [len=75] (mis=3)
75-425 ==> 0-350 on |468|TRAV14/DV4|L-REGION+V-REGION| [len=350] (mis=5)
431-490 ==> 7-66 on |418|TRAJ32|J-REGION| [len=66] (mis=0)
490-532 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 1 umis using 13 reads
cdr3 = CAMRGGGVGGATNKLIF at 408, score = 7 + 8
umis assigned: [294, 306]
of which 1 are surviving nonsolos
reads assigned: 548
start codons at 75, 165, 208, 229, 267, 295, 307, 399, 414
confident = true

TIG 2[bases=510]
0-52 ==> 10-62 on |565|TRBV12-3|5'UTR| [len=62] (mis=0)
52-399 ==> 0-347 on |566|TRBV12-3|L-REGION+V-REGION| [len=347] (mis=5)
407-458 ==> 0-51 on |539|TRBJ1-4|J-REGION| [len=51] (mis=5)
458-510 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 4 umis using 320 reads
cdr3 = CASSPLAGWFSEKLFF at 382, score = 8 + 8
umis assigned: [106, 116, 179, 220, 259]
of which 4 are surviving nonsolos
reads assigned: 3967
start codons at 0, 52, 110, 137, 151, 174, 223, 226, 275, 286, 313, 320
confident = true
now this is a cell
paired!

GACTCAGCAATGTATTTCTGTGCAATGAGGGGGGGCGGAGTTGGTGGTGCTACAAACAAGCTCATCTTTGGAACTGGCACTCTGCTTGCTGTCCAGCCAA <==> CCCAGGGACTCAGCTGTGTACTTCTGTGCCAGCAGTCCCCTAGCAGGCTGGTTTAGTGAAAAACTGTTTTTTGGCAGTGGAACCCAGCTCTCTGTCTTGG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1102 = TGATTTCAGTGTGAAT-1

using 70 reads

====================================================================================

graph has 64 edges initially, 4 edges after simplification

total ucounts = 12
nonsolo ucounts = 9[2, 3, 4^2, 6, 7, 8, 9, 24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1104 = TGATTTCAGTTACCCA-1

using 35 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1107 = TGATTTCCAACTGGCC-1

using 371 reads

====================================================================================

graph has 250 edges initially, 12 edges after simplification

total ucounts = 27
nonsolo ucounts = 20[2^3, 3^2, 4^2, 7, 8, 10, 14, 15^3, 19, 22, 34^2, 48, 103]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1110 = TGATTTCCAAGCGCTC-1

using 152 reads

====================================================================================

graph has 128 edges initially, 4 edges after simplification

total ucounts = 13
nonsolo ucounts = 11[2, 3, 5, 6, 8, 9^2, 13, 16, 30, 49]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1111 = TGATTTCCAAGGACTG-1

using 528 reads

====================================================================================

graph has 296 edges initially, 6 edges after simplification

total ucounts = 20
nonsolo ucounts = 13[2^4, 3, 5, 7^3, 8, 13, 16, 447]
surviving nonsolo ucounts = 1[447]
ids = [7]

====================================================================================

UMI info for barcode TGATTTCCAAGGACTG-1 contig 1 = AGCAGATCTC...
umi CCTTGGAGCG = 445 reads: +403 validated

GOOD CONTIGS

TIG 1[bases=552]
0-97 ==> 15-112 on |618|TRBV30|5'UTR| [len=112] (mis=0)
97-432 ==> 0-335 on |619|TRBV30|L-REGION+V-REGION| [len=335] (mis=4)
450-500 ==> 0-50 on |547|TRBJ2-4|J-REGION| [len=50] (mis=1)
500-552 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=1)
junction support: 1 umis using 27 reads
cdr3 = CAWSNVGGGGSAKNIQYF at 418, score = 7 + 8
umis assigned: [7]
of which 1 are surviving nonsolos
reads assigned: 439
start codons at 94, 97, 165
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1112 = TGATTTCCAAGTAATG-1

using 71 reads

====================================================================================

graph has 54 edges initially, 2 edges after simplification

total ucounts = 12
nonsolo ucounts = 5[2^3, 13, 45]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1114 = TGATTTCCAATAGCGG-1

using 114 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[114]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1116 = TGATTTCCAATGGATA-1

using 38 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 6[2^4, 13, 14]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1122 = TGATTTCCACATAACC-1

using 34 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1125 = TGATTTCCACGAAGCA-1

using 119 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[119]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1126 = TGATTTCCACGGTAGA-1

using 111 reads

====================================================================================

graph has 54 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 8[4^2, 6^2, 7, 20, 29, 34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1127 = TGATTTCCACGGTTTA-1

using 50 reads

====================================================================================

graph has 56 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 5[2, 5, 6, 15, 19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1130 = TGATTTCCACTTCTGC-1

using 24 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1136 = TGATTTCCAGCCTGTG-1

using 63 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[63]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1137 = TGATTTCCAGCGATCC-1

using 44 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[44]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1138 = TGATTTCCAGCTGGCT-1

using 2317 reads

====================================================================================

graph has 680 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 5[4, 6^2, 8, 2293]
surviving nonsolo ucounts = 1[2293]
ids = [2]

====================================================================================

UMI info for barcode TGATTTCCAGCTGGCT-1 contig 1 = TCTTTCCCAG...
umi CTGAATTCTG = 2313 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=505]
0-59 ==> 250-309 on |630|TRBV5-1|5'UTR| [len=309] (mis=0)
59-402 ==> 0-343 on |631|TRBV5-1|L-REGION+V-REGION| [len=343] (mis=2)
403-453 ==> 0-50 on |540|TRBJ1-5|J-REGION| [len=50] (mis=4)
453-505 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 1 umis using 138 reads
cdr3 = CASSYGQNQPQHF at 386, score = 9 + 8
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 2275
start codons at 59, 341, 345, 429
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1140 = TGATTTCCAGCTGTTA-1

using 234 reads

====================================================================================

graph has 176 edges initially, 10 edges after simplification

total ucounts = 20
nonsolo ucounts = 18[2^5, 3^3, 4, 5, 6, 7, 13, 14, 21, 31, 47, 65]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1142 = TGATTTCCAGCTTCGG-1

using 31533 reads

====================================================================================

graph has 12675 edges initially, 274 edges after simplification

total ucounts = 904
nonsolo ucounts = 667[2^111, 3^69, 4^59, 5^33, 6^48, 7^31, 8^27, 9^33, 10^22, 11^24, 12^14, 13^11, 14^20, 15^16, 16^12, 17^9, 18^3, 19^6, 20^4, 21^6, 22^6, 23^4, 24^5, 25^2, 26, 27, 28^3, 29, 30^5, 31, 32^5, 33, 34^3, 35^3, 36, 37^6, 38, 39, 41, 43^2, 44, 45^2, 46, 49^3, 50, 51, 53, 54^3, 55, 57, 59^2, 60^2, 61, 63, 65, 66^2, 67, 68^2, 71, 72^2, 77^3, 81^2, 83, 85, 91, 93^2, 94, 108, 110, 125, 160, 322, 375, 762, 1037, 1148, 1180, 1381, 1513, 1976, 5577, 7373]
surviving nonsolo ucounts = 11[322, 375, 762, 1037, 1148, 1180, 1381, 1513, 1976, 5577, 7373]
ids = [710, 797, 541, 613, 845, 859, 668, 22, 630, 593, ...]

====================================================================================

UMI info for barcode TGATTTCCAGCTTCGG-1 contig 1 = AAAATGCCCC...
umi AACTAGGGGT = 1501 reads: +403 validated
umi AGGCAGGCAG = 6423 reads: -381 +2 -1XX +1 -1XX +4 -1XX +1 -1XX +5 -2XX +1 -1XX +1 invalidated
umi GGACAGTCGC = 686 reads: -384X +2 -2XX +2 -1XX +1 -4XX +2 -2XX +1 -1XX +1 invalidated
umi GTGGAAGGCC = 844 reads: +41 -5XX +357 invalidated
umi TAAAAGGTTA = 1964 reads: +403 validated
umi TCACACGAGC = 319 reads: +403 validated
umi TGGCGATATG = 322 reads: -391X +1 -4XX +2 -2XX +1 -1XX +1 invalidated
umi TTCTCAATTC = 1175 reads: +403 validated

UMI info for barcode TGATTTCCAGCTTCGG-1 contig 2 = GAGAAGCCTC...
umi TAGCGCGCTA = 1387 reads: +403 validated

GOOD CONTIGS

TIG 1[bases=529]
0-74 ==> 0-74 on |591|TRBV2|5'UTR| [len=74] (mis=0)
74-421 ==> 0-347 on |592|TRBV2|L-REGION+V-REGION| [len=347] (mis=3)
429-477 ==> 0-48 on |537|TRBJ1-2|J-REGION| [len=48] (mis=1)
477-529 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 5 umis using 296 reads
cdr3 = CASSQDSRFNYGYTF at 404, score = 6 + 9
umis assigned: [22, 129, 541, 613, 630, 710, 797, 859]
of which 8 are surviving nonsolos
reads assigned: 13140
start codons at 3, 31, 74, 91, 173, 285, 315, 342, 395, 435
confident = true

TIG 2[bases=562]
0-117 ==> 0-117 on |474|TRAV19|5'UTR| [len=117] (mis=1)
117-467 ==> 0-350 on |475|TRAV19|L-REGION+V-REGION| [len=350] (mis=4)
457-520 ==> 0-63 on |425|TRAJ39|J-REGION| [len=63] (mis=1)
520-562 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 1 umis using 46 reads
cdr3 = CALNDNAGNMLTF at 450, score = 7 + 9
umis assigned: [668]
of which 1 are surviving nonsolos
reads assigned: 1360
start codons at 111, 117, 174, 229, 250, 340, 349, 460, 466, 477, 507
confident = true

REJECT CONTIGS

TIG 1[bases=507]
0-70 ==> 0-70 on |465|TRAV13-2|5'UTR| [len=70] (mis=0)
70-410 ==> 0-340 on |466|TRAV13-2|L-REGION+V-REGION| [len=340] (mis=4)
419-465 ==> 8-54 on |430|TRAJ43|J-REGION| [len=54] (mis=0)
465-507 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
umis assigned: [593, 845]
of which 2 are surviving nonsolos
reads assigned: 4923
start codons at 2, 5, 20, 41, 67, 70, 94, 289, 420, 425
confident = false
frameshifted full length stopped transcript of length 507
did not find CDR3
now this is a cell
paired!

TCACAAGTCGTGGACTCAGCAGTATACTTCTGTGCTCTGAATGATAATGCAGGCAACATGCTCACCTTTGGAGGGGGAACAAGGTTAATGGTCAAACCCC <==> AAGCTGGAGGACTCAGCCATGTACTTCTGTGCCAGCAGTCAGGACAGTCGCTTTAACTATGGCTACACCTTCGGTTCGGGGACCAGGTTAACCGTTGTAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1146 = TGATTTCCAGGGATTG-1

using 26 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1147 = TGATTTCCATATGAGA-1

using 126 reads

====================================================================================

graph has 114 edges initially, 4 edges after simplification

total ucounts = 18
nonsolo ucounts = 15[2^2, 3^2, 4^2, 5^2, 7^2, 11, 12^2, 13, 33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1149 = TGATTTCCATCAGTCA-1

using 418 reads

====================================================================================

graph has 258 edges initially, 16 edges after simplification

total ucounts = 18
nonsolo ucounts = 15[2, 3, 4, 6^2, 16, 17, 18, 21, 26, 28, 46, 61, 73, 88]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1156 = TGATTTCGTAAGGGCT-1

using 59 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[7, 52]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1157 = TGATTTCGTACAAGTA-1

using 75 reads

====================================================================================

graph has 56 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[75]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1158 = TGATTTCGTACCGGCT-1

using 39 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 3[4, 13, 19]
surviving nonsolo ucounts = 1[19]
ids = [1]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1161 = TGATTTCGTAGATTAG-1

using 150 reads

====================================================================================

graph has 110 edges initially, 8 edges after simplification

total ucounts = 18
nonsolo ucounts = 14[2^2, 3, 4^3, 9^2, 10, 13^2, 15, 22, 36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1163 = TGATTTCGTAGGCATG-1

using 45 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 6[2, 3^2, 4, 5, 27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1165 = TGATTTCGTATGAAAC-1

using 100 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[100]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1167 = TGATTTCGTCACCCAG-1

using 48 reads

====================================================================================

graph has 30 edges initially, 4 edges after simplification

total ucounts = 4
nonsolo ucounts = 4[2, 4, 9, 33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1169 = TGATTTCGTCATTAGC-1

using 46 reads

====================================================================================

graph has 56 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 4[2, 3, 18^2]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1170 = TGATTTCGTCCGAGTC-1

using 56 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[56]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1171 = TGATTTCGTCGGATCC-1

using 28 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1173 = TGATTTCGTCTAGTCA-1

using 42 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 7[2^2, 3, 6, 8^2, 11]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1179 = TGATTTCGTGTCTGAT-1

using 48 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 13
nonsolo ucounts = 7[2, 3, 5, 7^2, 9^2]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1181 = TGATTTCGTGTGCGTC-1

using 42 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 3[6, 9, 23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1182 = TGATTTCGTTAAGGGC-1

using 35 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 7[2^2, 3, 4, 5, 8^2]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1186 = TGATTTCGTTCACCTC-1

using 41 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[40]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1188 = TGATTTCGTTGGGACA-1

using 2357 reads

====================================================================================

graph has 714 edges initially, 4 edges after simplification

total ucounts = 6
nonsolo ucounts = 3[4, 27, 2323]
surviving nonsolo ucounts = 1[2323]
ids = [2]

====================================================================================

UMI info for barcode TGATTTCGTTGGGACA-1 contig 1 = GGGGATCCTG...
umi GGACACGGGA = 2335 reads: +403 validated

GOOD CONTIGS

TIG 1[bases=478]
23-370 ==> 0-347 on |688|TRBV7-9|L-REGION+V-REGION| [len=347] (mis=3)
368-380 ==> 0-12 on |535|TRBD1|D-REGION| [len=12] (mis=3)
379-426 ==> 0-47 on |550|TRBJ2-7|J-REGION| [len=47] (mis=2)
426-478 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 1 umis using 166 reads
cdr3 = CASSQGPGRAYEQYF at 353, score = 7 + 8
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 2297
start codons at 23, 47, 132, 231, 344
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1189 = TGATTTCGTTGTCTTT-1

using 61 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 3[2, 8, 51]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1190 = TGATTTCGTTTCCACC-1

using 26 reads

====================================================================================

graph has 4 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1192 = TGATTTCTCAAACAAG-1

using 29 reads

====================================================================================

graph has 6 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1200 = TGATTTCTCATGGTCA-1

using 115 reads

====================================================================================

graph has 56 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[115]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1203 = TGATTTCTCCAATGGT-1

using 68 reads

====================================================================================

graph has 54 edges initially, 2 edges after simplification

total ucounts = 14
nonsolo ucounts = 9[2^3, 4^2, 5, 6, 12, 26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1204 = TGATTTCTCCACGACG-1

using 54 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 5[2, 3, 4^2, 37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1206 = TGATTTCTCCCGGATG-1

using 50 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[50]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1209 = TGATTTCTCCGCAGTG-1

using 116 reads

====================================================================================

graph has 70 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[116]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1211 = TGATTTCTCCTACAGA-1

using 34 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1212 = TGATTTCTCCTATGTT-1

using 68 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[68]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1214 = TGATTTCTCCTGTACC-1

using 28 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1215 = TGATTTCTCCTGTAGA-1

using 1264 reads

====================================================================================

graph has 422 edges initially, 4 edges after simplification

total ucounts = 9
nonsolo ucounts = 6[2, 3^2, 8, 40, 1205]
surviving nonsolo ucounts = 1[1205]
ids = [2]

====================================================================================

UMI info for barcode TGATTTCTCCTGTAGA-1 contig 1 = ACAGTGATCC...
umi ATAATCATAG = 1209 reads: +400 validated

GOOD CONTIGS

TIG 1[bases=499]
0-47 ==> 0-47 on |674|TRBV7-2|5'UTR| [len=47] (mis=0)
47-394 ==> 0-347 on |675|TRBV7-2|L-REGION+V-REGION| [len=347] (mis=5)
400-447 ==> 0-47 on |550|TRBJ2-7|J-REGION| [len=47] (mis=3)
447-499 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 1 umis using 72 reads
cdr3 = CASSRNRENYEQYF at 377, score = 8 + 8
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 1189
start codons at 47, 156
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1216 = TGATTTCTCCTTTCTC-1

using 56 reads

====================================================================================

graph has 52 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[56]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1218 = TGATTTCTCGATCCCT-1

using 62 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 5[3, 10, 11, 16, 22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1221 = TGATTTCTCGGATGTT-1

using 67 reads

====================================================================================

graph has 55 edges initially, 4 edges after simplification

total ucounts = 8
nonsolo ucounts = 7[2, 5, 9, 11, 12, 13, 14]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1224 = TGATTTCTCGGTTCGG-1

using 49 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 8[2, 3, 4^2, 5, 8, 10, 11]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1225 = TGATTTCTCGTACGGC-1

using 96 reads

====================================================================================

graph has 88 edges initially, 4 edges after simplification

total ucounts = 13
nonsolo ucounts = 7[2^2, 4, 6, 12, 26, 38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1227 = TGATTTCTCGTCCGTT-1

using 68 reads

====================================================================================

graph has 82 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 7[2, 4, 5, 6, 11^2, 27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1230 = TGATTTCTCTACTATC-1

using 98 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[98]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1231 = TGATTTCTCTATCCCG-1

using 79 reads

====================================================================================

graph has 70 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 6[2, 5, 7, 11, 20, 34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1232 = TGATTTCTCTCGATGA-1

using 48 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 8[2, 3^4, 5, 11, 17]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1234 = TGATTTCTCTCTGTCG-1

using 54 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[54]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1236 = TGATTTCTCTGCAAGT-1

using 138 reads

====================================================================================

graph has 106 edges initially, 2 edges after simplification

total ucounts = 12
nonsolo ucounts = 11[2^3, 4, 5, 9, 10, 11, 15, 23, 54]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1238 = TGATTTCTCTGGGCCA-1

using 13 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[13]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1239 = TGATTTCTCTGGTGTA-1

using 227 reads

====================================================================================

graph has 154 edges initially, 2 edges after simplification

total ucounts = 17
nonsolo ucounts = 9[2, 3^2, 4, 6, 9, 11, 16, 165]
surviving nonsolo ucounts = 1[165]
ids = [5]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1240 = TGATTTCTCTGTCTAT-1

using 47 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[47]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1244 = TGATTTCTCTTTAGGG-1

using 83 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[83]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1245 = TGCACCTAGAAACGCC-1

using 105 reads

====================================================================================

graph has 82 edges initially, 4 edges after simplification

total ucounts = 10
nonsolo ucounts = 8[2^4, 3, 13, 28, 51]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1248 = TGCACCTAGAACTGTA-1

using 101 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[101]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1254 = TGCACCTAGACGACGT-1

using 29876 reads

====================================================================================

graph has 9296 edges initially, 208 edges after simplification

total ucounts = 916
nonsolo ucounts = 672[2^109, 3^69, 4^54, 5^44, 6^37, 7^34, 8^35, 9^30, 10^22, 11^27, 12^20, 13^22, 14^20, 15^10, 16^12, 17^10, 18^6, 19^7, 20^5, 21^7, 22^2, 23^4, 24^5, 25^7, 26^5, 27^3, 28^3, 29^6, 30^6, 31^4, 32^6, 33^5, 34, 35^3, 36, 37^2, 38, 40^2, 41, 43^3, 44^4, 46, 47, 49, 50, 51, 54^3, 55, 56, 57, 67, 85, 239, 467, 4933, 7780, 9020]
surviving nonsolo ucounts = 5[239, 467, 4933, 7780, 9020]
ids = [742, 826, 202, 759, 66]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1255 = TGCACCTAGACTGTAA-1

using 41 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[41]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1256 = TGCACCTAGAGAACAG-1

using 450 reads

====================================================================================

graph has 292 edges initially, 14 edges after simplification

total ucounts = 34
nonsolo ucounts = 27[2^7, 3, 4, 5^2, 6, 7, 9, 10, 11, 13, 16^2, 24, 26, 30, 31, 43, 52, 58, 60]
surviving nonsolo ucounts = 1[26]
ids = [3]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1257 = TGCACCTAGAGAGCTC-1

using 197 reads

====================================================================================

graph has 152 edges initially, 4 edges after simplification

total ucounts = 12
nonsolo ucounts = 7[3, 4, 6, 8, 29, 51, 91]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1259 = TGCACCTAGATACACA-1

using 8613 reads

====================================================================================

graph has 4056 edges initially, 62 edges after simplification

total ucounts = 430
nonsolo ucounts = 303[2^59, 3^41, 4^26, 5^23, 6^18, 7^18, 8^8, 9^14, 10^10, 11^8, 12^10, 13^9, 14^7, 15^4, 16^4, 17^4, 18^3, 19, 20^2, 21^3, 22, 23, 25, 26^2, 27^3, 28, 29^2, 31^2, 32^2, 33, 37, 39^2, 40, 41, 42, 44, 45, 48, 66, 67, 70, 1674, 1953, 2058]
surviving nonsolo ucounts = 3[1674, 1953, 2058]
ids = [359, 67, 301]

====================================================================================

UMI info for barcode TGCACCTAGATACACA-1 contig 1 = ATCAATGCAC...
umi ACTTCAGCCA = 1921 reads: +394 validated
umi GGGTCAAAGA = 2046 reads: +394 validated
umi TCGCAGATAA = 1669 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=492]
0-46 ==> 0-46 on |661|TRBV6-6|5'UTR| [len=46] (mis=0)
46-390 ==> 0-344 on |662|TRBV6-6|L-REGION+V-REGION| [len=344] (mis=7)
387-399 ==> 0-12 on |535|TRBD1|D-REGION| [len=12] (mis=3)
392-440 ==> 0-48 on |537|TRBJ1-2|J-REGION| [len=48] (mis=5)
440-492 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 3 umis using 346 reads
cdr3 = CASRPGQGYGYTF at 373, score = 9 + 9
umis assigned: [67, 301, 359]
of which 3 are surviving nonsolos
reads assigned: 5539
start codons at 4, 46, 104, 157, 181, 196, 223, 287, 398
confident = true
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1264 = TGCACCTAGATCGGGT-1

using 34933 reads

====================================================================================

graph has 11333 edges initially, 222 edges after simplification

total ucounts = 742
nonsolo ucounts = 570[2^69, 3^55, 4^51, 5^25, 6^37, 7^21, 8^26, 9^26, 10^15, 11^21, 12^17, 13^13, 14^11, 15^12, 16^13, 17^9, 18^7, 19^9, 20^10, 21^8, 22^8, 23^4, 24^7, 25^10, 26^5, 27^4, 28^3, 29^3, 30, 31^3, 32^4, 33, 34^2, 35, 36^2, 37^4, 38^4, 39^3, 40, 42, 43^3, 44^3, 45^3, 46^3, 48, 50^2, 52, 53^2, 58, 60, 62, 63, 73, 77, 82, 83^2, 85, 150, 1057, 1455, 1473, 1683, 1722, 1776, 1786, 1827, 1838, 1869, 2041, 2117, 2118, 2232, 2441]
surviving nonsolo ucounts = 15[1057, 1455, 1473, 1683, 1722, 1776, 1786, 1827, 1838, 1869, 2041, 2117, 2118, 2232, 2441]
ids = [729, 529, 451, 260, 184, 4, 113, 693, 722, 67, ...]

====================================================================================

UMI info for barcode TGCACCTAGATCGGGT-1 contig 1 = AGAATGCTTA...
umi AAACGAACAC = 1779 reads: +397 validated
umi ACCACCACCA = 1871 reads: +397 validated
umi AGAGCTGGTC = 1788 reads: +397 validated
umi ATCCTAAGGC = 2144 reads: +397 validated
umi ATTCCTCCCA = 1734 reads: +397 validated
umi CCCCCCCCGC = 1686 reads: +397 validated
umi CCGCCAACTC = 2049 reads: +397 validated
umi GCGTATTAAA = 1 reads: -397X invalidated
umi GCTGGGCTTC = 1476 reads: +397 validated
umi GGGAAACATT = 2452 reads: +397 validated
umi TTATAATCCT = 1826 reads: +397 validated
umi TTGGCGTCCT = 1842 reads: +397 validated
umi TTTGAACTCT = 2238 reads: +397 validated

UMI info for barcode TGCACCTAGATCGGGT-1 contig 2 = GATCTTAATT...
umi CCCTCTTAGT = 2058 reads: +391 validated
umi GTTCATCGGT = 1393 reads: +391 validated

GOOD CONTIGS

TIG 1[bases=496]
0-47 ==> 0-47 on |692|TRBV9|5'UTR| [len=47] (mis=0)
47-390 ==> 0-343 on |693|TRBV9|L-REGION+V-REGION| [len=343] (mis=2)
399-444 ==> 8-53 on |541|TRBJ1-6|J-REGION| [len=53] (mis=0)
444-496 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 12 umis using 1725 reads
cdr3 = CASSAAVRDSPLHF at 374, score = 9 + 9
umis assigned: [4, 67, 113, 161, 184, 260, 272, 438, 451, 472] and 3 others
of which 12 are surviving nonsolos
reads assigned: 22538
start codons at 3, 47, 169, 255
confident = true

TIG 2[bases=456]
0-23 ==> 0-23 on |463|TRAV13-1|5'UTR| [len=23] (mis=0)
23-360 ==> 0-337 on |464|TRAV13-1|L-REGION+V-REGION| [len=337] (mis=2)
360-414 ==> 0-54 on |430|TRAJ43|J-REGION| [len=54] (mis=3)
414-456 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 2 umis using 157 reads
cdr3 = CAASGDNNDMRF at 347, score = 9 + 8
umis assigned: [268, 529]
of which 2 are surviving nonsolos
reads assigned: 3394
start codons at 23, 78, 87, 237, 369, 374
confident = true

REJECT CONTIGS

TIG 1[bases=522]
0-85 ==> 12-97 on |492|TRAV27|5'UTR| [len=97] (mis=0)
85-413 ==> 0-328 on |493|TRAV27|L-REGION+V-REGION| [len=328] (mis=2)
414-480 ==> 0-66 on |418|TRAJ32|J-REGION| [len=66] (mis=5)
480-522 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
umis assigned: [729]
of which 1 are surviving nonsolos
reads assigned: 1047
start codons at 58, 67, 85, 129, 237, 338, 420
confident = false
frameshifted full length transcript of length 522
did not find CDR3
now this is a cell
paired!

GAGACCCAACCTGAAGACTCGGCTGTCTACTTCTGTGCAGCAAGTGGGGATAACAATGACATGCGCTTTGGAGCAGGGACCAGACTGACAGTAAAACCAA <==> CTGGAGCTGGGGGACTCAGCTTTGTATTTCTGTGCCAGCAGCGCCGCGGTCAGAGATTCACCCCTCCACTTTGGGAACGGGACCAGGCTCACTGTGACAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1272 = TGCACCTAGCCTCGTG-1

using 14 reads

====================================================================================

graph has 6 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[14]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1277 = TGCACCTAGCTAAGAT-1

using 105 reads

====================================================================================

graph has 101 edges initially, 8 edges after simplification

total ucounts = 18
nonsolo ucounts = 11[2^4, 7^2, 9, 11, 12, 13, 31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1280 = TGCACCTAGCTCCTTC-1

using 178 reads

====================================================================================

graph has 82 edges initially, 6 edges after simplification

total ucounts = 3
nonsolo ucounts = 3[26, 46, 106]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1284 = TGCACCTAGGATATAC-1

using 60 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[60]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1291 = TGCACCTAGGGTGTTG-1

using 302 reads

====================================================================================

graph has 156 edges initially, 14 edges after simplification

total ucounts = 14
nonsolo ucounts = 12[3, 5, 6, 10, 14, 21, 22^2, 24, 44, 46, 83]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1293 = TGCACCTAGGTAGCTG-1

using 22 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1299 = TGCACCTAGTAGATGT-1

using 33 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1302 = TGCACCTAGTCCATAC-1

using 43 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[43]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1303 = TGCACCTAGTCCTCCT-1

using 85 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[85]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1305 = TGCACCTAGTCTTGCA-1

using 86 reads

====================================================================================

graph has 70 edges initially, 4 edges after simplification

total ucounts = 10
nonsolo ucounts = 6[2, 4, 6, 12, 24, 34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1307 = TGCACCTAGTGCCAGA-1

using 184 reads

====================================================================================

graph has 128 edges initially, 4 edges after simplification

total ucounts = 12
nonsolo ucounts = 7[2^2, 5, 11, 17, 37, 105]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1309 = TGCACCTAGTGTTTGC-1

using 48 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 6[2, 3^3, 12, 20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1310 = TGCACCTAGTTATCGC-1

using 59 reads

====================================================================================

graph has 6 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[59]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1312 = TGCACCTAGTTCGATC-1

using 21 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 4[2, 3^2, 9]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1314 = TGCACCTAGTTTCCTT-1

using 35 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1316 = TGCACCTCAAACTGCT-1

using 210 reads

====================================================================================

graph has 80 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[210]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1318 = TGCACCTCAAAGCGGT-1

using 44 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[44]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1321 = TGCACCTCAACACCTA-1

using 89 reads

====================================================================================

graph has 112 edges initially, 2 edges after simplification

total ucounts = 14
nonsolo ucounts = 11[2^2, 4, 6^2, 7^2, 11^2, 12, 18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1322 = TGCACCTCAAGAAAGG-1

using 38 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1325 = TGCACCTCAAGCTGGA-1

using 88 reads

====================================================================================

graph has 144 edges initially, 2 edges after simplification

total ucounts = 21
nonsolo ucounts = 15[2^2, 3^4, 4^3, 5^2, 6, 8, 9, 21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1328 = TGCACCTCAAGTTGTC-1

using 51 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 5[4^2, 6, 8, 24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1329 = TGCACCTCAATAGCAA-1

using 124 reads

====================================================================================

graph has 176 edges initially, 4 edges after simplification

total ucounts = 21
nonsolo ucounts = 17[2^6, 3^3, 4^2, 8^2, 10, 17, 19, 29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1334 = TGCACCTCAATGGTCT-1

using 56 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[56]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1335 = TGCACCTCAATGTAAG-1

using 35 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1341 = TGCACCTCACAGCGTC-1

using 26 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 2[5, 17]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1351 = TGCACCTCACGAAAGC-1

using 80 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[80]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1352 = TGCACCTCACGGACAA-1

using 109 reads

====================================================================================

graph has 124 edges initially, 2 edges after simplification

total ucounts = 15
nonsolo ucounts = 7[2, 5, 10, 11^2, 15, 47]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1356 = TGCACCTCAGACAAGC-1

using 66 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[66]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1358 = TGCACCTCAGACGCCT-1

using 91 reads

====================================================================================

graph has 48 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[91]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1360 = TGCACCTCAGACGTAG-1

using 38 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1361 = TGCACCTCAGAGCCAA-1

using 133 reads

====================================================================================

graph has 78 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[133]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1364 = TGCACCTCAGATGAGC-1

using 72 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[72]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1365 = TGCACCTCAGCAGTTT-1

using 18796 reads

====================================================================================

graph has 7628 edges initially, 185 edges after simplification

total ucounts = 964
nonsolo ucounts = 723[2^134, 3^94, 4^72, 5^66, 6^37, 7^45, 8^27, 9^37, 10^27, 11^22, 12^22, 13^14, 14^8, 15^10, 16^9, 17^14, 18^11, 19^7, 20^9, 21^6, 22^4, 23^2, 24^3, 25^4, 27^3, 29^3, 30^3, 31^2, 33^3, 34, 35^2, 38, 39, 41, 45^2, 46, 50^2, 52, 56, 58, 59, 62, 88, 96, 108, 704, 959, 1727, 2779, 3051, 3079]
surviving nonsolo ucounts = 6[704, 959, 1727, 2779, 3051, 3079]
ids = [155, 768, 938, 514, 879, 720]

====================================================================================

UMI info for barcode TGCACCTCAGCAGTTT-1 contig 1 = CTCACAGTGA...
umi GAGACCGTAG = 2788 reads: +400 validated
umi TAAGATCTTA = 3099 reads: +400 validated
umi TTAAAGCCTC = 3051 reads: -40X +1 -4XX +355 invalidated
umi TTTAGCCGGA = 1712 reads: +400 validated

UMI info for barcode TGCACCTCAGCAGTTT-1 contig 2 = AACTGCTCTA...
umi AGCTAAGCTT = 702 reads: +382 validated
umi TCAAGCTTCA = 962 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=502]
0-50 ==> 0-50 on |678|TRBV7-3|5'UTR| [len=50] (mis=0)
50-397 ==> 0-347 on |679|TRBV7-3|L-REGION+V-REGION| [len=347] (mis=4)
401-450 ==> 0-49 on |546|TRBJ2-3|J-REGION| [len=49] (mis=0)
450-502 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 4 umis using 831 reads
cdr3 = CASSNRPSTDTQYF at 380, score = 8 + 8
umis assigned: [514, 720, 879, 938]
of which 4 are surviving nonsolos
reads assigned: 10495
start codons at 50, 159, 273
confident = true

TIG 2[bases=593]
0-169 ==> 40-209 on |489|TRAV26-1|5'UTR| [len=209] (mis=0)
169-497 ==> 0-328 on |490|TRAV26-1|L-REGION+V-REGION| [len=328] (mis=3)
494-551 ==> 0-57 on |405|TRAJ20|J-REGION| [len=57] (mis=4)
551-593 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 2 umis using 53 reads
cdr3 = CIVRAVNDYKLSF at 481, score = 8 + 8
umis assigned: [155, 768]
of which 2 are surviving nonsolos
reads assigned: 1640
start codons at 42, 169, 221, 247, 308, 314, 365, 380, 389, 394
confident = true
now this is a cell
paired!

GCTACGCTGAGAGACACTGCTGTGTACTATTGCATCGTCAGAGCGGTTAACGACTACAAGCTCAGCTTTGGAGCCGGAACCACAGTAACTGTAAGAGCAA <==> ACAGAGCGGGGGGACTCAGCCGTGTATCTCTGTGCCAGCAGCAATAGACCTAGCACAGATACGCAGTATTTTGGCCCAGGCACCCGGCTGACAGTGCTCG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1366 = TGCACCTCAGCTCCGA-1

using 43 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[43]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1381 = TGCACCTCAGTGACAG-1

using 29 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1382 = TGCACCTCAGTGAGTG-1

using 85 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[84]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1383 = TGCACCTCATACGCTA-1

using 121 reads

====================================================================================

graph has 94 edges initially, 10 edges after simplification

total ucounts = 17
nonsolo ucounts = 9[2^3, 7^2, 14, 19, 24, 36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1385 = TGCACCTCATCACGTA-1

using 66 reads

====================================================================================

graph has 108 edges initially, 2 edges after simplification

total ucounts = 18
nonsolo ucounts = 13[2^4, 3^2, 4, 5^2, 6^2, 8, 13]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1388 = TGCACCTCATCCAACA-1

using 37 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1389 = TGCACCTCATCCGGGT-1

using 49 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[49]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1392 = TGCACCTCATCGGTTA-1

using 19220 reads

====================================================================================

graph has 12233 edges initially, 429 edges after simplification

total ucounts = 1794
nonsolo ucounts = 1400[2^222, 3^140, 4^133, 5^100, 6^74, 7^76, 8^46, 9^58, 10^42, 11^54, 12^40, 13^26, 14^29, 15^35, 16^17, 17^22, 18^29, 19^13, 20^19, 21^17, 22^10, 23^23, 24^16, 25^12, 26^11, 27^13, 28^5, 29^13, 30^11, 31^9, 32^7, 33^6, 34^9, 35^5, 36^7, 37^3, 38^2, 39, 40, 42^3, 43^3, 44^2, 45^2, 46, 47^2, 48^3, 49, 50, 51^2, 52, 56, 57^2, 58^2, 62, 66^2, 67, 76^2, 77^2, 78, 92, 93, 95, 113, 119, 124, 158, 851, 1986]
surviving nonsolo ucounts = 5[77, 124, 158, 851, 1986]
ids = [1710, 1756, 730, 46, 933]

====================================================================================

UMI info for barcode TGCACCTCATCGGTTA-1 contig 1 = GGAACTGAAG...
umi AACGCTCTCG = 860 reads: +418 validated
umi CTACGACGTG = 157 reads: +139 -31 +56 -21 +56 -115 non-validated
umi GCAGTTCGGG = 1945 reads: -297X +1 -1XX +119 invalidated
umi TTGCTAGCGC = 61 reads: -413X +2 -1XX +2 invalidated
umi TTTGACACCT = 96 reads: -413X +2 -1XX +2 invalidated

GOOD CONTIGS

TIG 1[bases=864]
394-768 ==> 0-374 on |575|TRBV13|L-REGION+V-REGION| [len=374] (mis=3)
762-812 ==> 0-50 on |538|TRBJ1-3|J-REGION| [len=50] (mis=1)
812-864 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 2 umis using 157 reads
cdr3 = CASSSENTIYF at 751, score = 9 + 7
umis assigned: [46, 730, 933, 1710, 1756]
of which 5 are surviving nonsolos
reads assigned: 3067
start codons at 42, 54, 121, 145, 222, 272, 290, 394, 451, 546, 629, 637, 712
confident = true
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1393 = TGCACCTCATCTACGA-1

using 32 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1404 = TGCACCTCATGTTCCC-1

using 78 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[78]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1408 = TGCACCTCATTCACTT-1

using 22 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1410 = TGCACCTCATTGAGCT-1

using 85 reads

====================================================================================

graph has 82 edges initially, 4 edges after simplification

total ucounts = 9
nonsolo ucounts = 4[2, 10, 24, 44]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1411 = TGCACCTCATTGGGCC-1

using 20 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1414 = TGCACCTGTAAGAGAG-1

using 166 reads

====================================================================================

graph has 88 edges initially, 4 edges after simplification

total ucounts = 7
nonsolo ucounts = 5[2, 5, 20, 37, 100]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1418 = TGCACCTGTAATCGTC-1

using 61 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[7, 54]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1422 = TGCACCTGTACCGGCT-1

using 80000 reads

====================================================================================

graph has 44398 edges initially, 1784 edges after simplification

total ucounts = 4869
nonsolo ucounts = 4050[2^498, 3^362, 4^303, 5^231, 6^177, 7^146, 8^126, 9^122, 10^98, 11^114, 12^96, 13^72, 14^63, 15^70, 16^55, 17^61, 18^55, 19^51, 20^48, 21^24, 22^53, 23^46, 24^47, 25^46, 26^43, 27^47, 28^41, 29^30, 30^40, 31^39, 32^36, 33^33, 34^41, 35^30, 36^30, 37^32, 38^34, 39^23, 40^26, 41^32, 42^34, 43^27, 44^19, 45^31, 46^28, 47^20, 48^20, 49^21, 50^27, 51^28, 52^19, 53^18, 54^16, 55^18, 56^20, 57^15, 58^14, 59^12, 60^18, 61^12, 62^9, 63^7, 64^11, 65^7, 66^4, 67^7, 68^4, 69^6, 70^3, 71^5, 72^3, 73^5, 74^6, 75^4, 76^4, 77^2, 78^2, 79, 81^5, 82^3, 84^3, 85, 87, 88, 89^2, 104, 106, 129, 141, 263, 6980]
surviving nonsolo ucounts = 1[6980]
ids = [2435]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1424 = TGCACCTGTACGACCC-1

using 85 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[85]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1429 = TGCACCTGTAGTAGTA-1

using 20 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1430 = TGCACCTGTATAATGG-1

using 112 reads

====================================================================================

graph has 96 edges initially, 2 edges after simplification

total ucounts = 20
nonsolo ucounts = 14[2^5, 3, 4^4, 13, 16, 18, 30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1441 = TGCACCTGTCATACTG-1

using 437 reads

====================================================================================

graph has 432 edges initially, 16 edges after simplification

total ucounts = 25
nonsolo ucounts = 24[2^4, 3^2, 4^3, 5^2, 7, 8, 17, 18, 19, 21, 22, 28, 31, 36, 47, 68, 78]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1443 = TGCACCTGTCATCGGC-1

using 77 reads

====================================================================================

graph has 106 edges initially, 6 edges after simplification

total ucounts = 10
nonsolo ucounts = 8[2, 3, 4, 7, 9^2, 15, 26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1445 = TGCACCTGTCCAGTAT-1

using 1870 reads

====================================================================================

graph has 700 edges initially, 6 edges after simplification

total ucounts = 23
nonsolo ucounts = 17[2^3, 3^2, 4^2, 5^2, 6, 7^2, 10, 11, 13, 20, 1760]
surviving nonsolo ucounts = 1[1760]
ids = [2]

====================================================================================

UMI info for barcode TGCACCTGTCCAGTAT-1 contig 1 = GGAGGCAGTG...
umi AACGAGCTTC = 1761 reads: +406 validated

GOOD CONTIGS

TIG 1[bases=513]
0-55 ==> 23-78 on |597|TRBV20-1|5'UTR| [len=78] (mis=0)
55-390 ==> 0-335 on |598|TRBV20-1|L-REGION+V-REGION| [len=335] (mis=2)
393-409 ==> 0-16 on |730|TRBD2|D-REGION| [len=16] (mis=0)
412-461 ==> 0-49 on |546|TRBJ2-3|J-REGION| [len=49] (mis=2)
461-513 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 1 umis using 86 reads
cdr3 = CSANDPGLAGGPGADTQYF at 376, score = 7 + 8
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 1734
start codons at 55, 193, 229, 235, 248, 308, 386
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1451 = TGCACCTGTCCTGCTT-1

using 93 reads

====================================================================================

graph has 44 edges initially, 4 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[40, 51]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1453 = TGCACCTGTCGACTAT-1

using 30 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1455 = TGCACCTGTCGAGATG-1

using 50 reads

====================================================================================

graph has 58 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 4[3, 7, 18, 19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1463 = TGCACCTGTCTAGCGC-1

using 28 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1464 = TGCACCTGTCTCCATC-1

using 1939 reads

====================================================================================

graph has 616 edges initially, 4 edges after simplification

total ucounts = 12
nonsolo ucounts = 7[2, 3^2, 6, 23, 29, 1868]
surviving nonsolo ucounts = 1[1868]
ids = [11]

====================================================================================

UMI info for barcode TGCACCTGTCTCCATC-1 contig 1 = CCAGGAGGAC...
umi TCTCCTTCGT = 1880 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=499]
0-53 ==> 256-309 on |630|TRBV5-1|5'UTR| [len=309] (mis=0)
53-396 ==> 0-343 on |631|TRBV5-1|L-REGION+V-REGION| [len=343] (mis=2)
407-447 ==> 9-49 on |546|TRBJ2-3|J-REGION| [len=49] (mis=0)
447-499 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 1 umis using 109 reads
cdr3 = CASSSPPSETQYF at 380, score = 9 + 8
umis assigned: [11]
of which 1 are surviving nonsolos
reads assigned: 1842
start codons at 53, 335, 339
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1467 = TGCACCTGTCTTCTCG-1

using 44 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[44]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1468 = TGCACCTGTCTTGTCC-1

using 23 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 3[3, 9, 11]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1473 = TGCACCTGTGCATCTA-1

using 40 reads

====================================================================================

graph has 48 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 4[2, 3, 4, 24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1475 = TGCACCTGTGCCTGGT-1

using 87 reads

====================================================================================

graph has 126 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 7[2^2, 6, 7, 15, 23, 30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1480 = TGCACCTGTGGTAACG-1

using 24 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1481 = TGCACCTGTGGTGTAG-1

using 19333 reads

====================================================================================

graph has 9669 edges initially, 258 edges after simplification

total ucounts = 1092
nonsolo ucounts = 815[2^131, 3^91, 4^75, 5^54, 6^54, 7^49, 8^43, 9^37, 10^22, 11^26, 12^28, 13^23, 14^10, 15^13, 16^13, 17^12, 18^15, 19^5, 20^13, 21^13, 22^4, 23^6, 24^6, 25, 26^3, 27^5, 28^5, 29^2, 30^5, 31^3, 33^4, 34, 35^3, 36^5, 37^5, 40, 42^3, 44, 45^2, 51^2, 54^2, 57^2, 58, 63^2, 79, 80, 82, 86^2, 102, 187, 325, 511, 544, 864, 1581, 2678, 4122]
surviving nonsolo ucounts = 8[187, 325, 511, 544, 864, 1581, 2678, 4122]
ids = [643, 987, 115, 686, 967, 431, 991, 317]

====================================================================================

UMI info for barcode TGCACCTGTGGTGTAG-1 contig 1 = GGGGAGTAAA...
umi AGACATTCGG = 512 reads: +397 validated
umi TGTCTGGCGG = 872 reads: +397 validated
umi TTATGTTGGG = 2690 reads: +397 validated

UMI info for barcode TGCACCTGTGGTGTAG-1 contig 2 = GTCATCCCTC...
umi CCAGTGCGGT = 4133 reads: +397 validated
umi CGGTTGTTCC = 1584 reads: +397 validated
umi GGCCTCGCCT = 186 reads: +381 -1 +15 non-validated
umi GTAGTAGCCA = 555 reads: +397 validated
umi TTATCGTTTA = 325 reads: +397 validated

GOOD CONTIGS

TIG 1[bases=484]
45-382 ==> 0-337 on |500|TRAV34|L-REGION+V-REGION| [len=337] (mis=1)
381-442 ==> 8-69 on |439|TRAJ52|J-REGION| [len=69] (mis=0)
442-484 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 3 umis using 274 reads
cdr3 = CGADGGTSYGKLTF at 369, score = 7 + 9
umis assigned: [115, 967, 991]
of which 3 are surviving nonsolos
reads assigned: 4013
start codons at 45, 193, 220, 243, 246, 277, 301, 352, 394
confident = true

TIG 2[bases=527]
0-78 ==> 0-78 on |597|TRBV20-1|5'UTR| [len=78] (mis=0)
78-413 ==> 0-335 on |598|TRBV20-1|L-REGION+V-REGION| [len=335] (mis=0)
432-475 ==> 7-50 on |543|TRBJ2-1|J-REGION| [len=50] (mis=0)
475-527 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 4 umis using 405 reads
cdr3 = CSARDAGTRVTNEQFF at 399, score = 7 + 8
umis assigned: [317, 431, 643, 686, 987]
of which 5 are surviving nonsolos
reads assigned: 6675
start codons at 21, 78, 216, 252, 258, 271, 331, 412, 433
confident = true
now this is a cell
paired!

CAGCCCAGCCATGCAGGCATCTACCTCTGTGGAGCAGACGGTGGTACTAGCTATGGAAAGCTGACATTTGGACAAGGGACCATCTTGACTGTCCATCCAA <==> CCTGAAGACAGCAGCTTCTACATCTGCAGTGCTAGAGATGCCGGGACTCGGGTGACAAATGAGCAGTTCTTCGGGCCAGGGACACGGCTCACCGTGCTAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1482 = TGCACCTGTGTGAATA-1

using 77 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[77]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1483 = TGCACCTGTGTGACGA-1

using 114 reads

====================================================================================

graph has 112 edges initially, 4 edges after simplification

total ucounts = 13
nonsolo ucounts = 9[2, 3^2, 4, 6, 7, 10, 37, 38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1486 = TGCACCTGTTAAAGAC-1

using 59 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[59]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1492 = TGCACCTGTTATTCTC-1

using 87 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[87]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1495 = TGCACCTGTTCGCGAC-1

using 31 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[2, 29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1497 = TGCACCTGTTGAACTC-1

using 34 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[5, 29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1500 = TGCACCTGTTGTCGCG-1

using 65 reads

====================================================================================

graph has 128 edges initially, 6 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[65]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1501 = TGCACCTGTTTAGCTG-1

using 134 reads

====================================================================================

graph has 104 edges initially, 8 edges after simplification

total ucounts = 11
nonsolo ucounts = 7[2, 3, 4, 10, 27, 37, 47]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1504 = TGCACCTGTTTGTTTC-1

using 24 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1505 = TGCACCTTCAACACCA-1

using 280 reads

====================================================================================

graph has 142 edges initially, 6 edges after simplification

total ucounts = 14
nonsolo ucounts = 10[2^2, 3^2, 10, 15, 16, 52, 63, 110]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1506 = TGCACCTTCAACACTG-1

using 25 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1508 = TGCACCTTCAACGAAA-1

using 86 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[86]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1509 = TGCACCTTCAAGATCC-1

using 39 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[39]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1512 = TGCACCTTCACCACCT-1

using 22 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1523 = TGCACCTTCAGCGATT-1

using 93 reads

====================================================================================

graph has 58 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[2, 5, 15, 69]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1524 = TGCACCTTCAGCTGGC-1

using 49 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[49]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1526 = TGCACCTTCAGGTTCA-1

using 71 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[71]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1527 = TGCACCTTCAGTCCCT-1

using 109 reads

====================================================================================

graph has 118 edges initially, 4 edges after simplification

total ucounts = 19
nonsolo ucounts = 16[2^4, 3^3, 4, 5, 6^2, 7^2, 12, 19, 23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1530 = TGCACCTTCATAAAGG-1

using 178 reads

====================================================================================

graph has 196 edges initially, 4 edges after simplification

total ucounts = 31
nonsolo ucounts = 17[2^3, 3^3, 4^2, 5, 9, 10, 11, 12, 21, 23, 24, 26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1531 = TGCACCTTCATAGCAC-1

using 80 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[80]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1535 = TGCACCTTCATTTGGG-1

using 133 reads

====================================================================================

graph has 44 edges initially, 4 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[64, 69]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1541 = TGCACCTTCCATGAGT-1

using 44 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 2[2, 39]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1543 = TGCACCTTCCCGACTT-1

using 78 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[78]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1545 = TGCACCTTCCGCAGTG-1

using 112 reads

====================================================================================

graph has 86 edges initially, 2 edges after simplification

total ucounts = 17
nonsolo ucounts = 11[2^2, 3^3, 5^2, 8, 9, 28, 38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1546 = TGCACCTTCCGCATAA-1

using 31 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1548 = TGCACCTTCCTAAGTG-1

using 116 reads

====================================================================================

graph has 130 edges initially, 2 edges after simplification

total ucounts = 12
nonsolo ucounts = 9[2^3, 4, 8, 10, 11, 25, 49]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1550 = TGCACCTTCCTCAACC-1

using 32 reads

====================================================================================

graph has 52 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 4[2, 4^2, 21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1551 = TGCACCTTCCTCATTA-1

using 70 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[69]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1556 = TGCACCTTCCTTGGTC-1

using 35 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[2, 33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1558 = TGCACCTTCGAATCCA-1

using 75 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[75]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1560 = TGCACCTTCGCCAAAT-1

using 153 reads

====================================================================================

graph has 227 edges initially, 2 edges after simplification

total ucounts = 14
nonsolo ucounts = 11[2^3, 4^2, 7, 11, 14, 15, 17, 72]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1562 = TGCACCTTCGGAATCT-1

using 22 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1564 = TGCACCTTCGGCGCAT-1

using 65 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[65]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1565 = TGCACCTTCGGCGGTT-1

using 80 reads

====================================================================================

graph has 92 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[80]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1566 = TGCACCTTCGGCTACG-1

using 312 reads

====================================================================================

graph has 272 edges initially, 12 edges after simplification

total ucounts = 28
nonsolo ucounts = 21[2^4, 3^2, 5^2, 6, 9, 10, 14, 15^2, 16, 18, 20^2, 30, 47, 61]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1569 = TGCACCTTCGTCCAGG-1

using 108 reads

====================================================================================

graph has 68 edges initially, 4 edges after simplification

total ucounts = 8
nonsolo ucounts = 6[2, 4, 9, 11, 12, 68]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1574 = TGCACCTTCTACCAGA-1

using 18 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1577 = TGCACCTTCTAGCACA-1

using 373 reads

====================================================================================

graph has 300 edges initially, 14 edges after simplification

total ucounts = 19
nonsolo ucounts = 15[2, 3, 5, 7, 9, 11^2, 15, 23, 26, 30, 38, 39, 45, 105]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1580 = TGCACCTTCTCAAGTG-1

using 5715 reads

====================================================================================

graph has 1936 edges initially, 6 edges after simplification

total ucounts = 309
nonsolo ucounts = 278[2^21, 3^12, 4^16, 5^10, 6^13, 7^8, 8^5, 9^5, 10^3, 11^5, 12^4, 13^9, 14^2, 15^4, 16^4, 17^7, 18^8, 19^10, 20^2, 21^2, 22^5, 23^10, 24^6, 25^5, 26^9, 27^8, 28^8, 29^6, 30^6, 31^5, 32^4, 33^4, 34^5, 35^3, 36^8, 37^6, 38^3, 39^2, 40, 41^3, 42^2, 43, 44, 46^3, 49^3, 55, 56, 57^2, 58, 60, 61^2, 67, 70, 86]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1586 = TGCACCTTCTGCAGTA-1

using 109 reads

====================================================================================

graph has 52 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[109]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1591 = TGCACCTTCTGGCGAC-1

using 22 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1593 = TGCACCTTCTGTCTAT-1

using 80 reads

====================================================================================

graph has 80 edges initially, 2 edges after simplification

total ucounts = 12
nonsolo ucounts = 9[2, 3^2, 4^2, 7, 15^2, 24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1594 = TGCACCTTCTTCAACT-1

using 51 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[12, 39]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1595 = TGCACCTTCTTCATGT-1

using 78 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[10, 68]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1596 = TGCACCTTCTTGTACT-1

using 72 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[72]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1597 = TGCACCTTCTTTACAC-1

using 103 reads

====================================================================================

graph has 42 edges initially, 4 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[103]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1602 = TGCCAAAAGAATTGTG-1

using 113 reads

====================================================================================

graph has 58 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[112]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1604 = TGCCAAAAGACCACGA-1

using 62 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[62]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1609 = TGCCAAAAGACGCTTT-1

using 26 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[2, 22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1610 = TGCCAAAAGACTAGAT-1

using 36 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1613 = TGCCAAAAGAGGTTGC-1

using 29 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 1[27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1616 = TGCCAAAAGAGTGAGA-1

using 15525 reads

====================================================================================

graph has 12564 edges initially, 538 edges after simplification

total ucounts = 1417
nonsolo ucounts = 1034[2^129, 3^85, 4^83, 5^70, 6^53, 7^51, 8^36, 9^37, 10^33, 11^29, 12^32, 13^16, 14^20, 15^30, 16^22, 17^25, 18^21, 19^14, 20^8, 21^13, 22^14, 23^12, 24^11, 25^8, 26^11, 27^10, 28^9, 29^13, 30^8, 31^8, 32^6, 33^5, 34^7, 35^5, 36^9, 37^5, 38^7, 39^8, 40^6, 41^2, 42^3, 43^4, 44, 45^4, 46, 47^2, 48^2, 49^3, 50^4, 51^3, 54^4, 55^2, 56^2, 57, 59, 61, 62, 64, 67^2, 69^2, 70^2, 71, 72, 74^3, 78, 80^2, 82, 83, 90^2, 91, 93, 96, 137, 149]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1621 = TGCCAAAAGCCCAGCT-1

using 222 reads

====================================================================================

graph has 62 edges initially, 4 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[12, 210]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1626 = TGCCAAAAGCTACCGC-1

using 53 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[53]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1628 = TGCCAAAAGCTAGTGG-1

using 78 reads

====================================================================================

graph has 77 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[3, 6, 7, 60]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1631 = TGCCAAAAGCTGTTCA-1

using 133 reads

====================================================================================

graph has 122 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[133]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1632 = TGCCAAAAGCTTCGCG-1

using 32 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[7, 24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1635 = TGCCAAAAGGACAGAA-1

using 159 reads

====================================================================================

graph has 148 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[159]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1636 = TGCCAAAAGGACCACA-1

using 661 reads

====================================================================================

graph has 288 edges initially, 4 edges after simplification

total ucounts = 7
nonsolo ucounts = 6[2, 3^2, 5, 48, 599]
surviving nonsolo ucounts = 1[599]
ids = [0]

====================================================================================

REJECT CONTIGS

TIG 1[bases=407]
0-24 ==> 323-347 on |592|TRBV2|L-REGION+V-REGION| [len=347] (mis=5)
83-305 ==> 0-222 on segment before TRBJ1-5 exon 1 [len=222] (mis=0)
305-355 ==> 0-50 on |540|TRBJ1-5|J-REGION| [len=50] (mis=0)
355-407 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
cdr3 = CHTHDALCSNQPQHF at 282, score = 3 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 585
start codons at 85, 190, 292, 295, 331
confident = false
not full
VJ delta = -256
delta too large
not full
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1639 = TGCCAAAAGGCAAAGA-1

using 24456 reads

====================================================================================

graph has 10674 edges initially, 212 edges after simplification

total ucounts = 509
nonsolo ucounts = 392[2^38, 3^30, 4^19, 5^18, 6^14, 7^14, 8^12, 9^10, 10^12, 11^9, 12^8, 13^11, 14^11, 15^14, 16^12, 17^6, 18^14, 19^4, 20^5, 21^8, 22^5, 23^7, 24^6, 25^6, 26^5, 27^8, 28^6, 29^2, 30^4, 31^5, 32^4, 33^3, 34^2, 35^2, 36^2, 37, 38, 39^3, 40^3, 41, 42^2, 43, 44, 45, 46, 47, 49^2, 50, 51^2, 52, 53^4, 54, 55, 56^2, 57^2, 58^2, 63, 65, 66^2, 67^2, 69, 71, 73, 76, 85, 109, 120, 271, 901, 1034, 1706, 1900, 2549, 2758, 2892, 3443]
surviving nonsolo ucounts = 10[34, 271, 901, 1034, 1706, 1900, 2549, 2758, 2892, 3443]
ids = [365, 44, 160, 114, 305, 313, 157, 18, 138, 417]

====================================================================================

UMI info for barcode TGCCAAAAGGCAAAGA-1 contig 1 = AGAGGCCCCA...
umi ACCGGGCATC = 2773 reads: -95X +308 invalidated
umi CCATTCCTCC = 2905 reads: +403 validated
umi CCTAAGTTGC = 2567 reads: +403 validated
umi GGTTTCGAGG = 1717 reads: +403 validated
umi GTCCAGATCT = 1903 reads: +403 validated

UMI info for barcode TGCCAAAAGGCAAAGA-1 contig 2 = TGGGGAGGAC...
umi CATCATCGCT = 1036 reads: +391 validated
umi TATGTGTAGT = 32 reads: +154 -1 +5 -2 +4 -1 +3 -1 +1 -1 +99 -91 +28 non-validated

GOOD CONTIGS

TIG 1[bases=484]
0-29 ==> 82-111 on |626|TRBV4-2|5'UTR| [len=111] (mis=0)
29-373 ==> 0-344 on |627|TRBV4-2|L-REGION+V-REGION| [len=344] (mis=1)
370-382 ==> 0-12 on |535|TRBD1|D-REGION| [len=12] (mis=3)
381-432 ==> 0-51 on |539|TRBJ1-4|J-REGION| [len=51] (mis=3)
432-484 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 5 umis using 969 reads
cdr3 = CASSQAQGDSNEKLFF at 356, score = 10 + 8
umis assigned: [18, 138, 157, 305, 313]
of which 5 are surviving nonsolos
reads assigned: 11609
start codons at 29, 83, 122, 128, 151, 179, 221, 286, 387
confident = true

TIG 2[bases=552]
119-456 ==> 0-337 on |458|TRAV12-1|L-REGION+V-REGION| [len=337] (mis=0)
453-510 ==> 0-57 on |416|TRAJ30|J-REGION| [len=57] (mis=2)
510-552 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 1 umis using 59 reads
cdr3 = CVVNNRDDKIIF at 443, score = 7 + 8
umis assigned: [114, 365]
of which 2 are surviving nonsolos
reads assigned: 1055
start codons at 21, 58, 116, 119, 216, 323, 345, 351, 462
confident = true

REJECT CONTIGS

TIG 1[bases=505]
0-55 ==> 23-78 on |597|TRBV20-1|5'UTR| [len=78] (mis=0)
55-390 ==> 0-335 on |598|TRBV20-1|L-REGION+V-REGION| [len=335] (mis=3)
403-453 ==> 0-50 on |543|TRBJ2-1|J-REGION| [len=50] (mis=0)
453-505 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
umis assigned: [44, 263, 417]
of which 2 are surviving nonsolos
reads assigned: 3649
start codons at 55, 193, 229, 235, 248, 308, 411
confident = false
did not find CDR3
now this is a cell
paired!

GACTCCAAGCTCAGTGATTCAGCCACCTACCTCTGTGTGGTGAACAACAGAGATGACAAGATCATCTTTGGAAAAGGGACACGACTTCATATTCTCCCCA <==> CCAGAAGACTCGGCCCTGTATCTCTGTGCCAGCAGCCAAGCACAGGGGGATTCTAATGAAAAACTGTTTTTTGGCAGTGGAACCCAGCTCTCTGTCTTGG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1641 = TGCCAAAAGGCCCTTG-1

using 224 reads

====================================================================================

graph has 96 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[224]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1643 = TGCCAAAAGGCGCTCT-1

using 68 reads

====================================================================================

graph has 60 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[3, 10, 53]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1644 = TGCCAAAAGGGAAACA-1

using 106 reads

====================================================================================

graph has 62 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[106]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1645 = TGCCAAAAGGTAGCTG-1

using 13 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[13]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1652 = TGCCAAAAGTCGATAA-1

using 45 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[45]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1653 = TGCCAAAAGTGAAGTT-1

using 24 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 2[3, 17]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1656 = TGCCAAAAGTGGCACA-1

using 209 reads

====================================================================================

graph has 74 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[209]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1659 = TGCCAAAAGTTACCCA-1

using 45 reads

====================================================================================

graph has 78 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[44]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1663 = TGCCAAACAACCGCCA-1

using 576 reads

====================================================================================

graph has 478 edges initially, 16 edges after simplification

total ucounts = 15
nonsolo ucounts = 15[2^2, 6, 10, 14, 19, 24, 29, 36, 43, 47, 80, 83, 90, 91]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1664 = TGCCAAACAACTTGAC-1

using 18 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1665 = TGCCAAACAAGAGTCG-1

using 26 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[4^2, 17]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1667 = TGCCAAACAAGGCTCC-1

using 49 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[49]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1672 = TGCCAAACAATAGCAA-1

using 53 reads

====================================================================================

graph has 68 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 8[2^4, 3^2, 14, 22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1674 = TGCCAAACAATCAGAA-1

using 81 reads

====================================================================================

graph has 90 edges initially, 2 edges after simplification

total ucounts = 12
nonsolo ucounts = 11[2^3, 3, 5^2, 6, 7, 8, 15, 25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1675 = TGCCAAACAATGAAAC-1

using 71 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[5, 9, 55]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1676 = TGCCAAACAATGGACG-1

using 1833 reads

====================================================================================

graph has 1790 edges initially, 50 edges after simplification

total ucounts = 257
nonsolo ucounts = 169[2^31, 3^21, 4^21, 5^14, 6^8, 7^11, 8^8, 9^8, 10^6, 12^7, 13^4, 14^4, 15^2, 16^2, 17^2, 18^3, 19^2, 20, 21, 23, 29, 33, 40^2, 41, 43, 61, 68, 72, 73, 76, 125]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1679 = TGCCAAACACACCGCA-1

using 56 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[56]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1682 = TGCCAAACACCAGATT-1

using 162 reads

====================================================================================

graph has 108 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 5[2, 6^2, 9, 135]
surviving nonsolo ucounts = 1[135]
ids = [5]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1683 = TGCCAAACACCAGGCT-1

using 41 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[41]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1686 = TGCCAAACACCTGGTG-1

using 53 reads

====================================================================================

graph has 78 edges initially, 2 edges after simplification

total ucounts = 12
nonsolo ucounts = 8[2, 3^2, 5^3, 7, 19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1687 = TGCCAAACACGAGGTA-1

using 37 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1694 = TGCCAAACAGCCAATT-1

using 61 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[61]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1696 = TGCCAAACAGCCTATA-1

using 72 reads

====================================================================================

graph has 60 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[72]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1702 = TGCCAAACAGGGTATG-1

using 46 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[46]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1706 = TGCCAAACAGTGGAGT-1

using 25 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 3[5, 6, 10]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1707 = TGCCAAACAGTTCATG-1

using 81 reads

====================================================================================

graph has 114 edges initially, 2 edges after simplification

total ucounts = 17
nonsolo ucounts = 13[2^3, 3^2, 4, 5, 6, 8^2, 9, 12, 13]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1710 = TGCCAAACATCTACGA-1

using 114 reads

====================================================================================

graph has 122 edges initially, 4 edges after simplification

total ucounts = 11
nonsolo ucounts = 8[2, 7^2, 10, 13, 15, 25, 32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1711 = TGCCAAACATCTGGTA-1

using 670 reads

====================================================================================

graph has 494 edges initially, 18 edges after simplification

total ucounts = 26
nonsolo ucounts = 24[3^2, 4^2, 5, 6, 8^2, 9^2, 14, 16, 19, 28, 34, 38, 42^2, 50, 53, 62, 63, 71, 77]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1714 = TGCCAAACATGCCACG-1

using 53 reads

====================================================================================

graph has 54 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[2, 12, 38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1716 = TGCCAAACATGCCTAA-1

using 10728 reads

====================================================================================

graph has 11504 edges initially, 328 edges after simplification

total ucounts = 1186
nonsolo ucounts = 928[2^128, 3^86, 4^66, 5^80, 6^56, 7^61, 8^42, 9^40, 10^27, 11^30, 12^17, 13^33, 14^28, 15^21, 16^20, 17^16, 18^14, 19^15, 20^17, 21^11, 22^16, 23^8, 24^11, 25^6, 26^9, 27^10, 28^3, 29^6, 30^4, 31^3, 33^2, 34^2, 35^3, 36^3, 37, 38, 39^3, 40, 44, 45, 46, 47^2, 48, 49, 50^2, 51^3, 52^2, 53, 54, 60, 61, 66^2, 69, 70^2, 72, 74, 79, 97, 115]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1718 = TGCCAAACATGGTCAT-1

using 34 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1719 = TGCCAAACATGTCCTC-1

using 28 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1720 = TGCCAAACATGTTGAC-1

using 49 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[49]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1727 = TGCCAAAGTAAAGTCA-1

using 32 reads

====================================================================================

graph has 56 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 3[3^2, 26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1733 = TGCCAAAGTACAGTGG-1

using 24464 reads

====================================================================================

graph has 13925 edges initially, 382 edges after simplification

total ucounts = 1394
nonsolo ucounts = 1010[2^144, 3^102, 4^86, 5^56, 6^48, 7^39, 8^41, 9^38, 10^32, 11^42, 12^30, 13^31, 14^31, 15^24, 16^21, 17^17, 18^16, 19^18, 20^18, 21^13, 22^13, 23^11, 24^8, 25^13, 26^9, 27^8, 28^7, 29^8, 30^3, 31^5, 32^4, 33^4, 34^2, 35^4, 36^3, 37^3, 38^2, 39^7, 40, 41^4, 42^4, 43^4, 44^2, 45^2, 46, 47, 48, 50^2, 52^3, 55, 56^3, 57, 58, 59, 62, 63, 65, 66, 69, 70, 73, 74, 75, 79, 88, 92, 93, 105, 106, 494, 10999]
surviving nonsolo ucounts = 2[494, 10999]
ids = [192, 881]

====================================================================================

REJECT CONTIGS

TIG 1[bases=400]
43-147 ==> 0-104 on segment before TRBJ2-3 exon 1 [len=104] (mis=0)
147-196 ==> 0-49 on |546|TRBJ2-3|J-REGION| [len=49] (mis=0)
196-298 ==> 0-102 on segment before TRBJ2-4 exon 1 [len=102] (mis=0)
348-400 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
umis assigned: [192, 881]
of which 2 are surviving nonsolos
reads assigned: 11284
start codons at 57
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1735 = TGCCAAAGTACCAGTT-1

using 34 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 5[2, 3^2, 4, 21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1736 = TGCCAAAGTACCCAAT-1

using 26 reads

====================================================================================

graph has 4 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1739 = TGCCAAAGTACGAAAT-1

using 36947 reads

====================================================================================

graph has 16852 edges initially, 485 edges after simplification

total ucounts = 1442
nonsolo ucounts = 1069[2^148, 3^102, 4^85, 5^55, 6^55, 7^39, 8^37, 9^35, 10^32, 11^31, 12^30, 13^26, 14^31, 15^31, 16^16, 17^24, 18^8, 19^20, 20^13, 21^24, 22^13, 23^19, 24^19, 25^8, 26^7, 27^11, 28^11, 29^4, 30^7, 31^11, 32^7, 33^16, 34^10, 35^6, 36^5, 37^4, 38, 39, 40^2, 41^4, 42^2, 43^5, 44^4, 46^4, 47^2, 48^3, 51^2, 52^3, 53, 54^5, 55^3, 56, 57, 58, 59^2, 61, 62^3, 65, 69, 79^2, 106, 107, 126, 195, 214, 216, 1045, 1078, 1520, 1818, 1971, 2726, 5217, 6339]
surviving nonsolo ucounts = 11[195, 214, 216, 1045, 1078, 1520, 1818, 1971, 2726, 5217, 6339]
ids = [26, 419, 925, 912, 593, 126, 1393, 260, 107, 417, ...]

====================================================================================

UMI info for barcode TGCCAAAGTACGAAAT-1 contig 1 = ACCTGGAGCC...
umi AAATCTACGG = 167 reads: -366X +2 -1X +5 -1X +2 -1X +2 -1X +8 -1X +5 -4XX +7 invalidated
umi ACAGAAATTG = 2740 reads: +406 validated
umi ATAATGATCA = 1968 reads: +406 validated
umi CAACATCTCC = 5764 reads: -369 +11 -2XX +1 -1XX +2 -1XX +2 -1XX +6 -3XX +5 -1XX +1 invalidated
umi CACTCACCAA = 4786 reads: -369 +11 -2XX +1 -1XX +2 -1XX +2 -1XX +6 -3XX +5 -1XX +1 invalidated
umi CACTCCTTGC = 178 reads: -397X +2 -6XX +1 invalidated
umi CTTTTTTTTC = 1 reads: -406X invalidated
umi GGCAGGGCTC = 173 reads: -386X +1 -2X +1 -1X +2 -4XX +2 -6XX +1 invalidated
umi TTGGTAACCT = 1820 reads: +406 validated

UMI info for barcode TGCCAAAGTACGAAAT-1 contig 2 = GGCTTTGTCG...
umi ACCATTTCGG = 1520 reads: +385 validated
umi CCTATCTGGC = 1088 reads: +385 validated
umi GCTATACTTT = 1043 reads: +385 validated
umi TGTAAGTGAC = 3 reads: -372X +2 -7XX +1 -2XX +1 invalidated

GOOD CONTIGS

TIG 1[bases=501]
0-43 ==> 0-43 on |610|TRBV27|5'UTR| [len=43] (mis=0)
43-387 ==> 0-344 on |611|TRBV27|L-REGION+V-REGION| [len=344] (mis=5)
388-400 ==> 0-12 on |535|TRBD1|D-REGION| [len=12] (mis=1)
401-449 ==> 0-48 on |548|TRBJ2-5|J-REGION| [len=48] (mis=2)
449-501 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 3 umis using 409 reads
cdr3 = CASRNAGTGGLQETQYF at 370, score = 7 + 7
umis assigned: [26, 107, 260, 375, 417, 419, 835, 925, 1393]
of which 8 are surviving nonsolos
reads assigned: 17348
start codons at 35, 43, 65, 178, 185, 193, 247, 251, 275, 383
confident = true

TIG 2[bases=560]
0-133 ==> 0-133 on |482|TRAV22|5'UTR| [len=133] (mis=0)
133-464 ==> 0-331 on |483|TRAV22|L-REGION+V-REGION| [len=331] (mis=1)
461-518 ==> 0-57 on |405|TRAJ20|J-REGION| [len=57] (mis=3)
518-560 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 3 umis using 138 reads
cdr3 = CAVEANDYKLSF at 451, score = 7 + 8
umis assigned: [126, 593, 912, 1296]
of which 3 are surviving nonsolos
reads assigned: 3581
start codons at 58, 97, 106, 133, 359
confident = true
now this is a cell
paired!

TCTTCCCAGACCACAGACTCAGGCGTTTATTTCTGTGCTGTGGAGGCTAACGACTACAAGCTCAGCTTTGGAGCCGGAACCACAGTAACTGTAAGAGCAA <==> AACCAGACCTCTCTGTACTTCTGTGCCAGCAGGAATGCCGGGACAGGGGGACTACAAGAGACCCAGTACTTCGGGCCAGGCACGCGGCTCCTGGTGCTCG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1745 = TGCCAAAGTATAATGG-1

using 15 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1749 = TGCCAAAGTCAAAGAT-1

using 1723 reads

====================================================================================

graph has 642 edges initially, 6 edges after simplification

total ucounts = 5
nonsolo ucounts = 4[2, 26, 40, 1654]
surviving nonsolo ucounts = 1[1654]
ids = [2]

====================================================================================

UMI info for barcode TGCCAAAGTCAAAGAT-1 contig 1 = AGAGGCCCCA...
umi CACATTCCGT = 1660 reads: +403 validated

GOOD CONTIGS

TIG 1[bases=484]
0-29 ==> 82-111 on |626|TRBV4-2|5'UTR| [len=111] (mis=0)
29-373 ==> 0-344 on |627|TRBV4-2|L-REGION+V-REGION| [len=344] (mis=1)
370-382 ==> 0-12 on |535|TRBD1|D-REGION| [len=12] (mis=3)
381-432 ==> 0-51 on |539|TRBJ1-4|J-REGION| [len=51] (mis=3)
432-484 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 1 umis using 125 reads
cdr3 = CASSQAQGDSNEKLFF at 356, score = 10 + 8
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 1627
start codons at 29, 83, 122, 128, 151, 179, 221, 286, 387
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1751 = TGCCAAAGTCACCTAA-1

using 285 reads

====================================================================================

graph has 154 edges initially, 12 edges after simplification

total ucounts = 13
nonsolo ucounts = 11[2, 6, 7, 10, 11, 23, 33, 43, 46^2, 56]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1757 = TGCCAAAGTCCAAGTT-1

using 188 reads

====================================================================================

graph has 148 edges initially, 6 edges after simplification

total ucounts = 16
nonsolo ucounts = 13[2^2, 3, 5^2, 7, 9, 11^2, 18, 24, 37, 51]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1762 = TGCCAAAGTCCTGCTT-1

using 91 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[91]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1765 = TGCCAAAGTCGCTTTC-1

using 25 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1769 = TGCCAAAGTCTCACCT-1

using 60 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[16, 44]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1772 = TGCCAAAGTCTCGTTC-1

using 2195 reads

====================================================================================

graph has 648 edges initially, 4 edges after simplification

total ucounts = 8
nonsolo ucounts = 3[4, 34, 2152]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1774 = TGCCAAAGTCTCTCTG-1

using 33 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[5, 27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1777 = TGCCAAAGTGAAAGAG-1

using 29 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1779 = TGCCAAAGTGCAGTAG-1

using 37 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 1[35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1780 = TGCCAAAGTGCCTGGT-1

using 14 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[14]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1784 = TGCCAAAGTGGTCCGT-1

using 88 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[88]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1787 = TGCCAAAGTGTCCTCT-1

using 46 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[46]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1788 = TGCCAAAGTGTCTGAT-1

using 22 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1792 = TGCCAAAGTTACGTCA-1

using 1108 reads

====================================================================================

graph has 354 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 5[2^2, 3^2, 1094]
surviving nonsolo ucounts = 1[1094]
ids = [8]

====================================================================================

UMI info for barcode TGCCAAAGTTACGTCA-1 contig 1 = ATGAGATCCT...
umi GTTAGTGTGC = 1045 reads: +403 validated

GOOD CONTIGS

TIG 1[bases=479]
0-24 ==> 0-24 on |558|TRBV10-3|5'UTR| [len=24] (mis=0)
24-368 ==> 0-344 on |559|TRBV10-3|L-REGION+V-REGION| [len=344] (mis=2)
377-427 ==> 0-50 on |540|TRBJ1-5|J-REGION| [len=50] (mis=2)
427-479 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 1 umis using 61 reads
cdr3 = CAISDFDRAPNQPQHF at 351, score = 10 + 8
umis assigned: [8]
of which 1 are surviving nonsolos
reads assigned: 1028
start codons at 0, 24, 46, 78, 82, 146, 174, 202, 229, 265, 403
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1796 = TGCCAAAGTTCCACAA-1

using 52 reads

====================================================================================

graph has 64 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[52]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1807 = TGCCAAAGTTTGACTG-1

using 28322 reads

====================================================================================

graph has 13037 edges initially, 296 edges after simplification

total ucounts = 970
nonsolo ucounts = 722[2^93, 3^69, 4^61, 5^48, 6^49, 7^17, 8^31, 9^18, 10^22, 11^19, 12^23, 13^18, 14^19, 15^20, 16^11, 17^13, 18^13, 19^20, 20^10, 21^14, 22^10, 23^9, 24^12, 25^10, 26^6, 27^5, 28, 29^3, 30, 31^6, 32^2, 33^5, 34^3, 35^3, 36^2, 37^3, 38^6, 39, 40, 41^5, 42, 43, 44, 45^2, 46^2, 47, 48^2, 49, 50^2, 51, 52, 56, 59^2, 62, 63, 64, 83, 85, 88, 89, 93, 142, 204, 725, 1043, 1251, 1274, 1410, 1600, 1708, 1799, 1832, 1929, 2007, 2215]
surviving nonsolo ucounts = 14[142, 204, 725, 1043, 1251, 1274, 1410, 1600, 1708, 1799, 1832, 1929, 2007, 2215]
ids = [392, 26, 432, 507, 225, 682, 299, 152, 555, 144, ...]

====================================================================================

UMI info for barcode TGCCAAAGTTTGACTG-1 contig 1 = ATTATTTTTT...
umi CGGCCCCGAA = 144 reads: +400 validated
umi TACTAGCCCC = 1244 reads: +400 validated

UMI info for barcode TGCCAAAGTTTGACTG-1 contig 2 = ATGCTCACAG...
umi AAGGATAAGA = 206 reads: +403 validated
umi ACATTCACGG = 2014 reads: +403 validated
umi ATAAACTGAG = 1675 reads: -366 +1 -1XX +2 -2XX +2 -1XX +2 -4XX +3 -1XX +1 -1XX +5 -1XX +2 -1XX +2 -4XX +1 invalidated
umi ATATAGGTCT = 1599 reads: +403 validated
umi ATTTCGTCTC = 1935 reads: +403 validated
umi CAATGTCGCC = 1267 reads: +403 validated
umi CCCTATCAGC = 2221 reads: +403 validated
umi CCTATGGGCC = 1848 reads: +403 validated
umi CTCGCAGCTC = 718 reads: +403 validated
umi GAGCGCGCGA = 1045 reads: +403 validated
umi GCTTCACGGA = 1708 reads: +403 validated

GOOD CONTIGS

TIG 1[bases=498]
0-56 ==> 0-56 on |461|TRAV12-3|5'UTR| [len=56] (mis=0)
56-399 ==> 0-343 on |462|TRAV12-3|L-REGION+V-REGION| [len=343] (mis=3)
393-456 ==> 0-63 on |431|TRAJ44|J-REGION| [len=63] (mis=4)
456-498 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 2 umis using 72 reads
cdr3 = CAMKRGTASKLTF at 386, score = 9 + 9
umis assigned: [392, 682]
of which 2 are surviving nonsolos
reads assigned: 1362
start codons at 56, 59, 221, 263, 294, 392
confident = true

TIG 2[bases=507]
0-52 ==> 10-62 on |565|TRBV12-3|5'UTR| [len=62] (mis=0)
52-393 ==> 0-341 on |566|TRBV12-3|L-REGION+V-REGION| [len=347] (mis=0)
404-455 ==> 0-51 on |544|TRBJ2-2|J-REGION| [len=51] (mis=3)
455-507 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 10 umis using 916 reads
cdr3 = CASSRRGQYTGELFF at 382, score = 8 + 9
umis assigned: [26, 65, 144, 152, 204, 225, 325, 349, 432, 507] and 1 others
of which 11 are surviving nonsolos
reads assigned: 15968
start codons at 0, 52, 110, 137, 151, 174, 223, 226, 275, 286, 313, 320
confident = true

REJECT CONTIGS

TIG 1[bases=519]
0-54 ==> 57-111 on |494|TRAV29/DV5|5'UTR| [len=111] (mis=1)
54-412 ==> 0-358 on |495|TRAV29/DV5|L-REGION+V-REGION| [len=358] (mis=4)
415-477 ==> 0-62 on |424|TRAJ38|J-REGION| [len=62] (mis=1)
477-519 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
cdr3 = CAAHTP at 399, score = 9 + 4
umis assigned: [299, 831]
of which 1 are surviving nonsolos
reads assigned: 1392
start codons at 54, 60, 127, 130, 216, 233, 301, 307, 417
confident = false
frameshifted full length stopped transcript of length 519
VJ delta = -5
not full
not full
now this is a cell
paired!

TCACAGCCCAGTGATTCAGCCACCTACCTCTGTGCAATGAAACGCGGCACTGCCAGTAAACTCACCTTTGGGACTGGAACAAGACTTCAGGTCACGCTCG <==> GAACCCAGGGACTCAGCTGTGTACTTCTGTGCCAGCAGCCGCAGGGGGCAATACACCGGGGAGCTGTTTTTTGGAGAAGGCTCTAGGCTGACCGTACTGG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1817 = TGCCAAATCACCACCT-1

using 51 reads

====================================================================================

graph has 10 edges initially, 4 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[19, 32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1818 = TGCCAAATCACGCATA-1

using 65 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[65]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1821 = TGCCAAATCAGCTTAG-1

using 19 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1823 = TGCCAAATCAGTGCAT-1

using 47 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[47]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1825 = TGCCAAATCATAACCG-1

using 76 reads

====================================================================================

graph has 70 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 6[2, 4, 7, 9, 16, 38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1826 = TGCCAAATCATCTGTT-1

using 94 reads

====================================================================================

graph has 72 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 5[2^2, 6, 39, 41]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1827 = TGCCAAATCATGTCTT-1

using 27 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1829 = TGCCAAATCCAAACAC-1

using 76 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[75]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1836 = TGCCAAATCCGTCAAA-1

using 2185 reads

====================================================================================

graph has 754 edges initially, 4 edges after simplification

total ucounts = 8
nonsolo ucounts = 5[2, 6, 10, 50, 2114]
surviving nonsolo ucounts = 1[2114]
ids = [7]

====================================================================================

UMI info for barcode TGCCAAATCCGTCAAA-1 contig 1 = ACAAGGGCCT...
umi TTTAAGCAGG = 2116 reads: +403 validated

GOOD CONTIGS

TIG 1[bases=500]
0-45 ==> 19-64 on |571|TRBV12-5|5'UTR| [len=64] (mis=1)
45-392 ==> 0-347 on |572|TRBV12-5|L-REGION+V-REGION| [len=347] (mis=1)
398-448 ==> 0-50 on |543|TRBJ2-1|J-REGION| [len=50] (mis=0)
448-500 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 1 umis using 135 reads
cdr3 = CASGLFKSSYNEQFF at 375, score = 8 + 8
umis assigned: [7]
of which 1 are surviving nonsolos
reads assigned: 2080
start codons at 45, 103, 144, 162, 167, 216, 219, 268, 279, 306, 313, 406
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1841 = TGCCAAATCCTTGCCA-1

using 68 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[68]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1842 = TGCCAAATCCTTTCGG-1

using 28 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1844 = TGCCAAATCGCCAAAT-1

using 49 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[49]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1846 = TGCCAAATCGCTAGCG-1

using 272 reads

====================================================================================

graph has 164 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[32, 240]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1847 = TGCCAAATCGGAGCAA-1

using 206 reads

====================================================================================

graph has 152 edges initially, 2 edges after simplification

total ucounts = 18
nonsolo ucounts = 16[2^3, 3^3, 6, 10, 13, 14, 15^2, 24^2, 34^2]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1849 = TGCCAAATCGGTGTCG-1

using 89 reads

====================================================================================

graph has 48 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[89]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1851 = TGCCAAATCTACTCAT-1

using 25 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1852 = TGCCAAATCTCACATT-1

using 35 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[11, 23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1855 = TGCCAAATCTCGCTTG-1

using 45 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[45]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1858 = TGCCAAATCTGCCAGG-1

using 50 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[50]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1859 = TGCCAAATCTGCCCTA-1

using 5449 reads

====================================================================================

graph has 5206 edges initially, 140 edges after simplification

total ucounts = 637
nonsolo ucounts = 466[2^76, 3^44, 4^31, 5^24, 6^35, 7^37, 8^20, 9^18, 10^12, 11^18, 12^17, 13^11, 14^4, 15^13, 16^11, 17^6, 18^9, 19^7, 20^8, 21^7, 22^3, 23^4, 24^5, 25^7, 26^2, 27^6, 28^2, 29^2, 30, 31, 32^2, 36, 37^3, 38, 39, 41, 42, 43, 44, 47, 49, 50, 53^2, 54, 56, 64, 67, 74, 82, 101, 113]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1863 = TGCCAAATCTTATCTG-1

using 41 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[4, 36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1864 = TGCCAAATCTTCCTTC-1

using 61 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[61]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1865 = TGCCAAATCTTCGAGA-1

using 10 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[10]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1869 = TGCCCATAGAAAGTGG-1

using 13428 reads

====================================================================================

graph has 5766 edges initially, 122 edges after simplification

total ucounts = 369
nonsolo ucounts = 267[2^38, 3^23, 4^18, 5^9, 6^13, 7^12, 8^11, 9^13, 10^7, 11^5, 12^7, 13^7, 14^7, 15^9, 16^5, 17^12, 18^5, 19^5, 20, 21^5, 22^4, 23^4, 24^3, 25^3, 26, 27^2, 28^3, 29^2, 30^2, 31, 32^2, 33^4, 34^3, 35^3, 36^3, 37, 39, 42, 44, 49, 52, 62, 79, 96, 846, 900, 932, 1064, 2402, 3775]
surviving nonsolo ucounts = 6[846, 900, 932, 1064, 2402, 3775]
ids = [68, 288, 327, 174, 25, 46]

====================================================================================

UMI info for barcode TGCCCATAGAAAGTGG-1 contig 1 = TGGGCTTCAA...
umi ACCACCCCTA = 2422 reads: +406 validated
umi AGGCCAACGT = 3799 reads: +406 validated
umi ATGCCCAATA = 162 reads: +258 -148 non-validated

UMI info for barcode TGCCCATAGAAAGTGG-1 contig 2 = GGGGAGTGAA...
umi TATATATTCT = 894 reads: +397 validated

GOOD CONTIGS

TIG 1[bases=475]
17-361 ==> 0-344 on |613|TRBV28|L-REGION+V-REGION| [len=344] (mis=2)
374-423 ==> 0-49 on |546|TRBJ2-3|J-REGION| [len=49] (mis=0)
423-475 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 2 umis using 385 reads
cdr3 = CASSLDLGPESTDTQYF at 344, score = 6 + 8
umis assigned: [25, 46, 68]
of which 3 are surviving nonsolos
reads assigned: 6264
start codons at 17, 75, 139, 152, 159, 167, 222, 225, 233, 335
confident = true

TIG 2[bases=488]
49-389 ==> 0-340 on |503|TRAV36/DV7|L-REGION+V-REGION| [len=340] (mis=3)
383-446 ==> 0-63 on |443|TRAJ57|J-REGION| [len=63] (mis=4)
446-488 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=2)
junction support: 1 umis using 43 reads
cdr3 = CAVLQGGSEKLVF at 376, score = 7 + 9
umis assigned: [288]
of which 1 are surviving nonsolos
reads assigned: 888
start codons at 49, 52, 185, 213, 253
confident = true

REJECT CONTIGS

TIG 1[bases=522]
4-419 ==> 118-533 on segment before TRAJ54 exon 1 [len=533] (mis=0)
420-480 ==> 0-60 on |441|TRAJ54|J-REGION| [len=60] (mis=1)
480-522 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
umis assigned: [174, 327]
of which 2 are surviving nonsolos
reads assigned: 1928
start codons at 122, 131, 174, 217, 256, 353
confident = false
did not find CDR3
now this is a cell
paired!

ACCCAGACCGGAGACTCGGCCATCTACCTCTGTGCTGTGCTGCAGGGCGGATCTGAAAAGCTGGTCTTTGGAAAGGGAACGAAACTGACAGTAAACCCAT <==> AACCAGACATCTATGTACCTCTGTGCCAGCAGTTTAGACTTGGGGCCGGAGAGCACAGATACGCAGTATTTTGGCCCAGGCACCCGGCTGACAGTGCTCG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1875 = TGCCCATAGAGCTGGT-1

using 32 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1881 = TGCCCATAGATATACG-1

using 66 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[66]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1884 = TGCCCATAGATGAGAG-1

using 28 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1887 = TGCCCATAGATGTTAG-1

using 38 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[5, 15, 16]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1889 = TGCCCATAGCAATCTC-1

using 101 reads

====================================================================================

graph has 88 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 4[2, 12, 14, 68]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1892 = TGCCCATAGCCCAGCT-1

using 80 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[5, 74]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1895 = TGCCCATAGCGGCTTC-1

using 65 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[65]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1905 = TGCCCATAGGATGGTC-1

using 46 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 1[44]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1907 = TGCCCATAGGCATGGT-1

using 89 reads

====================================================================================

graph has 96 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 9[2^2, 3, 4, 5, 7, 16, 22, 28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1915 = TGCCCATAGGGTCGAT-1

using 1621 reads

====================================================================================

graph has 1514 edges initially, 46 edges after simplification

total ucounts = 229
nonsolo ucounts = 162[2^39, 3^25, 4^18, 5^7, 6^7, 7^8, 8^10, 9^3, 10^3, 11^4, 12^5, 13^4, 14^3, 15^3, 16, 17^2, 18, 21, 22^2, 24, 26^3, 27, 28, 31^3, 32, 54, 58, 61, 66^2, 96]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1917 = TGCCCATAGTAATCCC-1

using 24 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1925 = TGCCCATAGTGCGTGA-1

using 34 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1928 = TGCCCATAGTTACCCA-1

using 393 reads

====================================================================================

graph has 250 edges initially, 6 edges after simplification

total ucounts = 10
nonsolo ucounts = 7[5, 33, 48, 64, 70, 78, 92]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1930 = TGCCCATAGTTCCACA-1

using 132 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[132]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1933 = TGCCCATCAAAGCAAT-1

using 15900 reads

====================================================================================

graph has 8391 edges initially, 278 edges after simplification

total ucounts = 651
nonsolo ucounts = 542[2^45, 3^48, 4^24, 5^24, 6^19, 7^20, 8^13, 9^20, 10^18, 11^14, 12^17, 13^14, 14^15, 15^6, 16^21, 17^13, 18^5, 19^8, 20^8, 21^6, 22^5, 23^9, 24^7, 25^2, 26^8, 27^4, 28^3, 29^6, 30^5, 31^3, 32^3, 33^7, 34^3, 35^9, 36, 37^4, 38^4, 39^3, 40^2, 41^4, 42^4, 43, 44^4, 45^2, 46, 47^4, 48^4, 49^2, 50^4, 51^2, 54^3, 55, 56, 57^2, 58^3, 59^3, 60^3, 61, 62^2, 63^3, 64^3, 65^3, 66, 67, 68^3, 69, 70, 71, 72, 73, 74^2, 75, 76^2, 77, 78, 80, 84, 85^2, 88, 90, 93^2, 94, 98, 101, 102, 105, 107, 115, 124, 614, 843, 2700]
surviving nonsolo ucounts = 4[70, 614, 843, 2700]
ids = [114, 551, 626, 374]

====================================================================================

UMI info for barcode TGCCCATCAAAGCAAT-1 contig 1 = ATTCTTTCTT...
umi AGTCACGATC = 9 reads: -400X invalidated
umi GCATAGTGTG = 2720 reads: +400 validated
umi TGCCTTTCCT = 2 reads: -400X invalidated
umi TTGAAACACG = 450 reads: +400 validated

UMI info for barcode TGCCCATCAAAGCAAT-1 contig 2 = CCAAAACAAG...
umi AAAGTCGGCT = 1 reads: -392X +1 -1X +1 -5X +1 -2X invalidated
umi TCCTGGTTCA = 587 reads: +403 validated
umi TTAAGCTCGC = 3 reads: -398X +1 -1XX +1 -1XX +1 invalidated

GOOD CONTIGS

TIG 1[bases=472]
0-20 ==> 0-20 on |612|TRBV28|5'UTR| [len=20] (mis=0)
20-364 ==> 0-344 on |613|TRBV28|L-REGION+V-REGION| [len=344] (mis=4)
370-420 ==> 0-50 on |540|TRBJ1-5|J-REGION| [len=50] (mis=3)
420-472 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 2 umis using 215 reads
cdr3 = CASSMQGAGNQPQHF at 347, score = 6 + 8
umis assigned: [114, 374, 583, 626]
of which 3 are surviving nonsolos
reads assigned: 3124
start codons at 20, 78, 142, 155, 162, 170, 225, 228, 236, 338, 359, 396
confident = true

TIG 2[bases=577]
0-132 ==> 0-132 on |518|TRAV8-1|5'UTR| [len=132] (mis=0)
132-473 ==> 0-341 on |519|TRAV8-1|L-REGION+V-REGION| [len=341] (mis=3)
481-535 ==> 8-62 on |423|TRAJ37|J-REGION| [len=62] (mis=0)
535-577 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 1 umis using 11 reads
cdr3 = CAVGALGGNTGKLIF at 459, score = 8 + 8
umis assigned: [9, 551, 599]
of which 1 are surviving nonsolos
reads assigned: 575
start codons at 132, 162, 181, 254, 268, 295
confident = true
now this is a cell
paired!

TGGAGTGACACAGCTGAGTACTTCTGTGCCGTGGGGGCATTAGGAGGCAACACAGGCAAACTAATCTTTGGGCAAGGGACAACTTTACAAGTAAAACCAG <==> AGCACCAACCAGACATCTATGTACCTCTGTGCCAGCAGTATGCAGGGGGCCGGTAATCAGCCCCAGCATTTTGGTGATGGGACTCGACTCTCCATCCTAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1934 = TGCCCATCAAATCCGT-1

using 33 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1937 = TGCCCATCAAGCCATT-1

using 24 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1944 = TGCCCATCAAGTCATC-1

using 21 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1945 = TGCCCATCAATAGCAA-1

using 77 reads

====================================================================================

graph has 106 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[3, 25, 48]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1947 = TGCCCATCAATCGAAA-1

using 32 reads

====================================================================================

graph has 27 edges initially, 7 edges after simplification

total ucounts = 3
nonsolo ucounts = 3[2^2, 28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1951 = TGCCCATCAATGTTGC-1

using 281 reads

====================================================================================

graph has 164 edges initially, 6 edges after simplification

total ucounts = 16
nonsolo ucounts = 11[2, 4^2, 5^2, 10^2, 20, 51, 81, 84]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1953 = TGCCCATCACAAGTAA-1

using 54 reads

====================================================================================

graph has 60 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 6[2^4, 8, 38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1955 = TGCCCATCACAGACTT-1

using 120 reads

====================================================================================

graph has 54 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 4[2^2, 17, 96]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1957 = TGCCCATCACAGCCCA-1

using 35 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[2, 33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1958 = TGCCCATCACAGGCCT-1

using 91 reads

====================================================================================

graph has 92 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 6[3, 4, 6, 8, 9, 59]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1959 = TGCCCATCACATCCAA-1

using 50 reads

====================================================================================

graph has 48 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 3[7, 10, 30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1961 = TGCCCATCACATTCGA-1

using 2642 reads

====================================================================================

graph has 742 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 3[2^2, 2631]
surviving nonsolo ucounts = 1[2631]
ids = [6]

====================================================================================

UMI info for barcode TGCCCATCACATTCGA-1 contig 1 = GGGCCTTTGT...
umi GAATCTCGCA = 2643 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=459]
13-349 ==> 0-336 on |631|TRBV5-1|L-REGION+V-REGION| [len=343] (mis=0)
359-407 ==> 0-48 on |536|TRBJ1-1|J-REGION| [len=48] (mis=1)
407-459 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 1 umis using 188 reads
cdr3 = CASGQLQNTEAFF at 340, score = 9 + 8
umis assigned: [6]
of which 1 are surviving nonsolos
reads assigned: 2604
start codons at 13, 295, 299
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1962 = TGCCCATCACATTTCT-1

using 70 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[69]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1970 = TGCCCATCAGACACTT-1

using 631 reads

====================================================================================

graph has 328 edges initially, 16 edges after simplification

total ucounts = 21
nonsolo ucounts = 17[2^2, 3^2, 6, 8, 11, 12, 14^2, 23, 31, 53, 84, 96, 115, 150]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1971 = TGCCCATCAGACGCCT-1

using 30 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1974 = TGCCCATCAGATGGGT-1

using 89 reads

====================================================================================

graph has 72 edges initially, 4 edges after simplification

total ucounts = 14
nonsolo ucounts = 6[2, 3, 10^2, 16, 40]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1975 = TGCCCATCAGATTGCT-1

using 91 reads

====================================================================================

graph has 66 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 5[3, 4, 5, 9, 67]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1980 = TGCCCATCAGCTGCTG-1

using 55 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[55]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1983 = TGCCCATCAGCTTAAC-1

using 98 reads

====================================================================================

graph has 164 edges initially, 2 edges after simplification

total ucounts = 18
nonsolo ucounts = 8[2^2, 3, 4, 6, 10, 16, 45]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1985 = TGCCCATCAGGATTGG-1

using 89 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[2, 87]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1988 = TGCCCATCAGGGATTG-1

using 163 reads

====================================================================================

graph has 94 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 8[2^2, 3, 4, 5, 12, 14, 118]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1989 = TGCCCATCAGGGCATA-1

using 73 reads

====================================================================================

graph has 72 edges initially, 4 edges after simplification

total ucounts = 7
nonsolo ucounts = 5[4, 11, 17, 19, 20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1993 = TGCCCATCAGTGAGTG-1

using 71 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[71]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1994 = TGCCCATCAGTTAACC-1

using 23 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1996 = TGCCCATCATCAGTAC-1

using 91 reads

====================================================================================

graph has 64 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[91]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1998 = TGCCCATCATCGATGT-1

using 51 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[2, 10, 16, 21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.1999 = TGCCCATCATCGGACC-1

using 19 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 1[17]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2001 = TGCCCATCATCGGTTA-1

using 33 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 1[31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2003 = TGCCCATCATGCAATC-1

using 37 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2006 = TGCCCATCATGGATGG-1

using 51 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 5[2, 3^3, 36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2014 = TGCCCATGTAACGACG-1

using 26 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 1[24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2015 = TGCCCATGTAATCGTC-1

using 16 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[16]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2031 = TGCCCATGTCAAAGAT-1

using 53 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[53]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2036 = TGCCCATGTCGAACAG-1

using 88 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[88]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2040 = TGCCCATGTCTCTCTG-1

using 98 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[98]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2043 = TGCCCATGTGCACCAC-1

using 64 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[64]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2044 = TGCCCATGTGCATCTA-1

using 22 reads

====================================================================================

graph has 6 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2048 = TGCCCATGTGGTTTCA-1

using 77 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[77]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2049 = TGCCCATGTGTGACGA-1

using 26 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2050 = TGCCCATGTGTGGCTC-1

using 158 reads

====================================================================================

graph has 128 edges initially, 6 edges after simplification

total ucounts = 9
nonsolo ucounts = 5[3, 12, 31, 34, 74]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2052 = TGCCCATGTGTTGAGG-1

using 43 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[43]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2053 = TGCCCATGTTAAGGGC-1

using 80 reads

====================================================================================

graph has 54 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[80]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2056 = TGCCCATGTTCAGACT-1

using 59 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[59]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2057 = TGCCCATGTTCAGGCC-1

using 47 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[47]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2058 = TGCCCATGTTCCACAA-1

using 85 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[85]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2060 = TGCCCATGTTGCGCAC-1

using 2410 reads

====================================================================================

graph has 842 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 6[2^3, 3, 5, 2394]
surviving nonsolo ucounts = 1[2394]
ids = [7]

====================================================================================

UMI info for barcode TGCCCATGTTGCGCAC-1 contig 1 = CAGATGCAGT...
umi TGCCAGTTTG = 2402 reads: +400 validated

GOOD CONTIGS

TIG 1[bases=482]
0-30 ==> 11-41 on |642|TRBV5-6|5'UTR| [len=41] (mis=0)
30-366 ==> 0-336 on |643|TRBV5-6|L-REGION+V-REGION| [len=343] (mis=0)
383-430 ==> 0-47 on |550|TRBJ2-7|J-REGION| [len=47] (mis=0)
430-482 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 1 umis using 169 reads
cdr3 = CASLKRGSLSYEQYF at 357, score = 8 + 8
umis assigned: [7]
of which 1 are surviving nonsolos
reads assigned: 2358
start codons at 3, 30, 152, 172, 235, 316
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2065 = TGCCCATGTTTGACAC-1

using 37 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2066 = TGCCCATTCAACGAAA-1

using 3941 reads

====================================================================================

graph has 2328 edges initially, 64 edges after simplification

total ucounts = 190
nonsolo ucounts = 147[2^20, 3^14, 4^10, 5^10, 6^7, 7^7, 8^5, 9^5, 10^4, 11^8, 12, 13, 14^5, 15^2, 16^2, 17^3, 18, 19, 20^3, 21, 22^4, 23^3, 24, 25, 27, 28, 29, 31, 32^3, 33^2, 35^2, 36^3, 37, 38, 39, 40, 46, 47, 65, 69, 73, 80, 81, 111, 792, 917]
surviving nonsolo ucounts = 2[36, 917]
ids = [67, 106]

====================================================================================

UMI info for barcode TGCCCATTCAACGAAA-1 contig 1 = GTCCTGTCTC...
umi GCACCATTCA = 914 reads: +409 validated

GOOD CONTIGS

TIG 1[bases=592]
0-183 ==> 25-208 on |589|TRBV19|5'UTR| [len=208] (mis=0)
183-527 ==> 0-344 on |590|TRBV19|L-REGION+V-REGION| [len=344] (mis=3)
523-535 ==> 0-12 on |535|TRBD1|D-REGION| [len=12] (mis=3)
541-592 ==> 0-51 on |539|TRBJ1-4|J-REGION| [len=51] (mis=0)
junction support: 1 umis using 9 reads
cdr3 = CASSSDRVREATNEKLFF at 510, score = 6 + 8
umis assigned: [106]
of which 1 are surviving nonsolos
reads assigned: 902
start codons at 183, 241, 292, 328, 333, 397, 547
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2067 = TGCCCATTCAATACCG-1

using 19 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2068 = TGCCCATTCACGCGGT-1

using 31 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2075 = TGCCCATTCAGTTTGG-1

using 3806 reads

====================================================================================

graph has 914 edges initially, 28 edges after simplification

total ucounts = 32
nonsolo ucounts = 29[2, 3^3, 4^2, 5^3, 8^2, 9, 10^2, 17, 18, 22, 25, 39, 42, 44, 49, 62, 69, 76, 77, 81, 85, 3018]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2077 = TGCCCATTCATCGGAT-1

using 67 reads

====================================================================================

graph has 74 edges initially, 2 edges after simplification

total ucounts = 17
nonsolo ucounts = 7[2^3, 5^2, 8, 33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2081 = TGCCCATTCCAAAGTC-1

using 20 reads

====================================================================================

graph has 4 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2083 = TGCCCATTCCACGAAT-1

using 77 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[76]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2086 = TGCCCATTCCAGAAGG-1

using 90 reads

====================================================================================

graph has 74 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 5[2^2, 10, 20, 52]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2087 = TGCCCATTCCAGGGCT-1

using 13 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[13]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2089 = TGCCCATTCCCACTTG-1

using 41 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[41]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2095 = TGCCCATTCCGTACAA-1

using 60 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[60]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2096 = TGCCCATTCCGTAGGC-1

using 50 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[50]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2099 = TGCCCATTCCTCATTA-1

using 38 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2101 = TGCCCATTCCTGCTTG-1

using 120 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[120]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2102 = TGCCCATTCCTTAATC-1

using 67 reads

====================================================================================

graph has 72 edges initially, 4 edges after simplification

total ucounts = 8
nonsolo ucounts = 6[3, 5, 6, 11, 19, 21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2103 = TGCCCATTCCTTCAAT-1

using 1981 reads

====================================================================================

graph has 636 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 3[11^2, 1956]
surviving nonsolo ucounts = 1[1956]
ids = [1]

====================================================================================

UMI info for barcode TGCCCATTCCTTCAAT-1 contig 1 = CTTTTCTCAG...
umi ACGTAAACGG = 1970 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=477]
0-31 ==> 36-67 on |614|TRBV29-1|5'UTR| [len=67] (mis=0)
31-366 ==> 0-335 on |615|TRBV29-1|L-REGION+V-REGION| [len=335] (mis=3)
377-425 ==> 0-48 on |537|TRBJ1-2|J-REGION| [len=48] (mis=1)
425-477 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 1 umis using 144 reads
cdr3 = CSVDLLGGINYGYTF at 352, score = 7 + 9
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 1930
start codons at 31, 166, 169, 245, 322, 383
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2104 = TGCCCATTCCTTGCCA-1

using 26 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2107 = TGCCCATTCGAATGCT-1

using 70 reads

====================================================================================

graph has 64 edges initially, 4 edges after simplification

total ucounts = 9
nonsolo ucounts = 6[2, 3, 9^2, 20, 24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2112 = TGCCCATTCGCCCTTA-1

using 126 reads

====================================================================================

graph has 80 edges initially, 4 edges after simplification

total ucounts = 9
nonsolo ucounts = 8[3^2, 4, 5^2, 13, 33, 59]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2115 = TGCCCATTCGGCCGAT-1

using 28 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2119 = TGCCCATTCGTGGGAA-1

using 393 reads

====================================================================================

graph has 290 edges initially, 12 edges after simplification

total ucounts = 14
nonsolo ucounts = 12[8, 11, 15, 17, 18^2, 22, 32, 47, 58, 63, 82]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2122 = TGCCCATTCTATGTGG-1

using 64 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[64]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2125 = TGCCCATTCTGCTGCT-1

using 83 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[83]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2126 = TGCCCATTCTGCTTGC-1

using 80 reads

====================================================================================

graph has 70 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 4[3^2, 4, 69]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2127 = TGCCCATTCTGGCGAC-1

using 76 reads

====================================================================================

graph has 110 edges initially, 2 edges after simplification

total ucounts = 14
nonsolo ucounts = 8[2^2, 3, 4, 5^2, 20, 29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2128 = TGCCCATTCTGTCAAG-1

using 103 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[103]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2131 = TGCCCATTCTTCATGT-1

using 70 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[70]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2132 = TGCCCATTCTTCGAGA-1

using 35 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2134 = TGCCCATTCTTGTATC-1

using 39 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[2, 5, 30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2139 = TGCCCTAAGAATGTGT-1

using 16 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[16]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2141 = TGCCCTAAGACACTAA-1

using 11701 reads

====================================================================================

graph has 8018 edges initially, 254 edges after simplification

total ucounts = 962
nonsolo ucounts = 741[2^91, 3^66, 4^51, 5^37, 6^41, 7^35, 8^41, 9^25, 10^30, 11^31, 12^25, 13^20, 14^21, 15^19, 16^18, 17^16, 18^19, 19^20, 20^13, 21^9, 22^10, 23^11, 24^7, 25^9, 26^5, 27^3, 28^5, 29^8, 30^4, 31^3, 32^2, 33^2, 34^6, 35, 36, 37^2, 38^4, 39^4, 40, 41^3, 43^2, 44, 47, 50, 51^2, 52^2, 56, 57, 61^2, 66, 75, 88^2, 93^2, 102, 315, 1956]
surviving nonsolo ucounts = 4[26, 66, 315, 1956]
ids = [265, 856, 825, 666]

====================================================================================

REJECT CONTIGS

TIG 1[bases=520]
0-64 ==> 0-64 on |573|TRBV12-5|5'UTR| [len=64] (mis=0)
64-411 ==> 0-347 on |574|TRBV12-5|L-REGION+V-REGION| [len=347] (mis=5)
420-432 ==> 0-12 on |535|TRBD1|D-REGION| [len=12] (mis=2)
424-468 ==> 7-51 on |544|TRBJ2-2|J-REGION| [len=51] (mis=0)
468-520 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
cdr3 = CIFVLVAYNGVWDGELFF at 386, score = 4 + 9
umis assigned: [666]
of which 1 are surviving nonsolos
reads assigned: 1926
start codons at 64, 122, 163, 181, 186, 235, 238, 287, 298, 325, 332
confident = false
not full
frameshifted full length stopped transcript of length 520
VJ delta = 28
not full
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2145 = TGCCCTAAGACGCTTT-1

using 96 reads

====================================================================================

graph has 152 edges initially, 4 edges after simplification

total ucounts = 16
nonsolo ucounts = 11[2^5, 3^2, 10, 16, 23, 26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2147 = TGCCCTAAGAGCTGGT-1

using 4188 reads

====================================================================================

graph has 1308 edges initially, 6 edges after simplification

total ucounts = 11
nonsolo ucounts = 5[2, 3, 6, 2032, 2139]
surviving nonsolo ucounts = 2[2032, 2139]
ids = [8, 2]

====================================================================================

UMI info for barcode TGCCCTAAGAGCTGGT-1 contig 1 = TGGGACAGTG...
umi ATCCACCTCT = 2148 reads: +394 validated

UMI info for barcode TGCCCTAAGAGCTGGT-1 contig 2 = AGACCAGAAT...
umi TATGATAGAG = 2027 reads: +400 validated

GOOD CONTIGS

TIG 1[bases=497]
51-398 ==> 0-347 on |688|TRBV7-9|L-REGION+V-REGION| [len=347] (mis=4)
398-445 ==> 0-47 on |550|TRBJ2-7|J-REGION| [len=47] (mis=3)
445-497 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 1 umis using 121 reads
cdr3 = CASTSSSDEQYF at 381, score = 7 + 8
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 2111
start codons at 51, 75, 160, 259, 372
confident = false

TIG 2[bases=499]
0-47 ==> 0-47 on |616|TRBV3-1|5'UTR| [len=47] (mis=0)
47-391 ==> 0-344 on |617|TRBV3-1|L-REGION+V-REGION| [len=344] (mis=0)
397-447 ==> 0-50 on |540|TRBJ1-5|J-REGION| [len=50] (mis=0)
447-499 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 1 umis using 163 reads
cdr3 = CASSQDRASNQPQHF at 374, score = 9 + 8
umis assigned: [8]
of which 1 are surviving nonsolos
reads assigned: 2000
start codons at 47, 146, 169, 189, 197, 239, 273, 423
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2153 = TGCCCTAAGATGTGGC-1

using 61 reads

====================================================================================

graph has 96 edges initially, 2 edges after simplification

total ucounts = 12
nonsolo ucounts = 10[2^2, 3, 4^2, 6^2, 7, 9, 16]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2154 = TGCCCTAAGCAACGGT-1

using 136 reads

====================================================================================

graph has 147 edges initially, 6 edges after simplification

total ucounts = 15
nonsolo ucounts = 11[2, 3, 6^2, 9, 10, 12, 13, 15, 22, 34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2155 = TGCCCTAAGCAGCCTC-1

using 208 reads

====================================================================================

graph has 128 edges initially, 4 edges after simplification

total ucounts = 19
nonsolo ucounts = 14[2^4, 3^3, 4, 7, 8, 13, 14, 36, 104]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2156 = TGCCCTAAGCAGGTCA-1

using 93 reads

====================================================================================

graph has 116 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 10[4^2, 5^2, 6, 7, 10, 13, 16, 22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2160 = TGCCCTAAGCGTCAAG-1

using 23 reads

====================================================================================

graph has 27 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[3, 7, 12]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2161 = TGCCCTAAGCTCCCAG-1

using 20 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2162 = TGCCCTAAGCTTCGCG-1

using 610 reads

====================================================================================

graph has 557 edges initially, 18 edges after simplification

total ucounts = 23
nonsolo ucounts = 22[3, 4, 5, 6^2, 7, 10, 12^2, 14, 16^3, 18, 27^3, 61, 65, 77, 89, 91]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2166 = TGCCCTAAGGACATTA-1

using 33 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2167 = TGCCCTAAGGACTGGT-1

using 35 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2168 = TGCCCTAAGGATATAC-1

using 35 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2169 = TGCCCTAAGGCATGGT-1

using 23 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2170 = TGCCCTAAGGCCCGTT-1

using 134 reads

====================================================================================

graph has 134 edges initially, 4 edges after simplification

total ucounts = 13
nonsolo ucounts = 8[2, 4^2, 6, 12, 20, 32, 49]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2171 = TGCCCTAAGGCTCAGA-1

using 98 reads

====================================================================================

graph has 108 edges initially, 4 edges after simplification

total ucounts = 12
nonsolo ucounts = 7[3^3, 9, 11, 19, 45]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2172 = TGCCCTAAGGGCATGT-1

using 17 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[17]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2174 = TGCCCTAAGGTGCTTT-1

using 40 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 3[5, 14, 21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2182 = TGCCCTAAGTGTGGCA-1

using 25 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2186 = TGCCCTAAGTTTCCTT-1

using 35 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2188 = TGCCCTACAAAGGTGC-1

using 82 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[82]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2191 = TGCCCTACAACACCTA-1

using 53 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[53]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2195 = TGCCCTACAAGCTGTT-1

using 77 reads

====================================================================================

graph has 60 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[77]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2197 = TGCCCTACAAGGTGTG-1

using 248 reads

====================================================================================

graph has 236 edges initially, 16 edges after simplification

total ucounts = 14
nonsolo ucounts = 11[2, 6, 7, 9, 13, 16, 19, 22, 44, 49, 58]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2198 = TGCCCTACAAGTACCT-1

using 186 reads

====================================================================================

graph has 101 edges initially, 4 edges after simplification

total ucounts = 15
nonsolo ucounts = 11[2^5, 3, 5, 15, 26, 60, 63]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2199 = TGCCCTACAATAACGA-1

using 30 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2208 = TGCCCTACACATTCGA-1

using 59 reads

====================================================================================

graph has 86 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 6[2^2, 3, 6, 8, 35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2210 = TGCCCTACACCATCCT-1

using 112 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[112]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2211 = TGCCCTACACCCAGTG-1

using 73 reads

====================================================================================

graph has 90 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 5[3, 5, 17, 21, 22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2218 = TGCCCTACACGTCTCT-1

using 83 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[83]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2222 = TGCCCTACAGACAAGC-1

using 13 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[13]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2227 = TGCCCTACAGATGGGT-1

using 86 reads

====================================================================================

graph has 124 edges initially, 2 edges after simplification

total ucounts = 22
nonsolo ucounts = 15[2^5, 3^4, 4, 5, 6, 7, 10, 25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2228 = TGCCCTACAGCATGAG-1

using 988 reads

====================================================================================

graph has 1110 edges initially, 41 edges after simplification

total ucounts = 142
nonsolo ucounts = 97[2^15, 3^10, 4^9, 5^10, 6^8, 7^8, 8^5, 9^3, 10, 11^4, 12^2, 13, 14, 16^3, 17^3, 18^2, 19, 21^4, 23^2, 26, 28, 34, 59, 90]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2233 = TGCCCTACAGCTTAAC-1

using 29 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2234 = TGCCCTACAGCTTCGG-1

using 52 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[52]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2240 = TGCCCTACAGTTCCCT-1

using 39 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 5[2^2, 6, 14^2]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2245 = TGCCCTACATCCTAGA-1

using 50 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[50]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2249 = TGCCCTACATCTATGG-1

using 60 reads

====================================================================================

graph has 88 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 5[2, 4, 6, 9, 34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2250 = TGCCCTACATGCTGGC-1

using 30 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2253 = TGCCCTACATTAACCG-1

using 74 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[74]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2256 = TGCCCTACATTCACTT-1

using 23 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2262 = TGCCCTAGTACATCCA-1

using 13 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[13]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2267 = TGCCCTAGTACTCAAC-1

using 24 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2273 = TGCCCTAGTATTCGTG-1

using 63 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 5[2^2, 6, 23, 28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2275 = TGCCCTAGTCAAACTC-1

using 6485 reads

====================================================================================

graph has 5498 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 2[5, 6475]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2284 = TGCCCTAGTCGTCTTC-1

using 8692 reads

====================================================================================

graph has 954 edges initially, 4 edges after simplification

total ucounts = 14
nonsolo ucounts = 10[2^3, 3, 4^2, 6, 15, 24, 8626]
surviving nonsolo ucounts = 1[8626]
ids = [5]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 88.2292 = TGCCCTAGTCTGATTG-1

using 48 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[2, 3, 41]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

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BARCODE 88.2294 = TGCCCTAGTCTTCAAG-1

using 40 reads

====================================================================================

graph has 48 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[40]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

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BARCODE 88.2297 = TGCCCTAGTGATGATA-1

using 32 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[12, 19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!
sorting bam, mem = 0.19
running samtools sort -l 8G -o /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_T_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk88-ucc6a7a72e6/files/contig_bam_sorted.bam /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_T_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk88-ucc6a7a72e6/files/contig_bam.bam

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98.402 seconds used processing barcodes, peak mem = 0.38
