[stdout]

starting vdj_asm_asm, mem = 0.01
available mem = 827.64 GB
/scratch/etanis/software/cellranger-6.1.2/lib/bin/cr_vdj martian assembly main /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_T_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk70-ucc6a7a72e6 /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_T_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk70-ucc6a7a72e6/files /scratch/etanis/ST-118/multi/dimitri-multi/journal/SC_MULTI_CS.SC_MULTI_CORE.MULTI_GEM_WELL_PROCESSOR.VDJ_T_GEM_WELL_PROCESSOR.SC_VDJ_CONTIG_ASSEMBLER.ASSEMBLE_VDJ.fork0.chnk70.ucc6a7a72e6
n50_n50_rpu = 2159

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.0 = GTCAAGTGTGATAAAC-1

using 64 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[64]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1 = GTCAAGTGTGGACGAT-1

using 47 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[47]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.3 = GTCAAGTGTGGGTCAA-1

using 47 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 4[2, 6, 7, 31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.4 = GTCAAGTGTGTCTGAT-1

using 22 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[5, 15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.11 = GTCAAGTGTTCGTGAT-1

using 29 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.13 = GTCAAGTGTTGTCGCG-1

using 686 reads

====================================================================================

graph has 470 edges initially, 8 edges after simplification

total ucounts = 102
nonsolo ucounts = 60[2^15, 3^5, 4^6, 5^5, 6^5, 7^2, 8^2, 9, 10, 11^2, 12, 13^3, 14, 16, 19, 21, 22, 23, 33^2, 34, 36, 38, 109]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.16 = GTCAAGTTCAACCATG-1

using 110 reads

====================================================================================

graph has 66 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 5[3, 4, 22, 39, 41]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.20 = GTCAAGTTCACAGTAC-1

using 48 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[2, 3, 14, 27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.22 = GTCAAGTTCACCGGGT-1

using 194 reads

====================================================================================

graph has 148 edges initially, 6 edges after simplification

total ucounts = 15
nonsolo ucounts = 11[2^2, 3, 5, 6, 7, 10, 12, 19, 30, 94]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.24 = GTCAAGTTCACTCCTG-1

using 53 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[53]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.26 = GTCAAGTTCAGCGATT-1

using 65 reads

====================================================================================

graph has 66 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 7[2^2, 5^2, 9, 19, 21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.36 = GTCAAGTTCCTTTCTC-1

using 75170 reads

====================================================================================

graph has 19930 edges initially, 358 edges after simplification

total ucounts = 1282
nonsolo ucounts = 976[2^143, 3^72, 4^75, 5^55, 6^38, 7^45, 8^37, 9^49, 10^28, 11^25, 12^22, 13^34, 14^39, 15^20, 16^18, 17^16, 18^24, 19^19, 20^19, 21^17, 22^18, 23^11, 24^16, 25^8, 26^11, 27^8, 28^14, 29^7, 30^5, 31^5, 32^3, 33^2, 34^4, 35^3, 36^5, 37^4, 38^3, 39, 40, 41, 42^2, 43, 44, 45, 46^2, 47, 49, 50, 53, 55, 58^2, 64, 69, 73, 75, 92, 131, 421, 557, 671, 915, 1003, 1047, 1063, 1266, 1275, 1429, 1570, 1605, 1616, 1666, 1851, 1874, 1884, 1896, 2029, 2115, 2151, 2162, 2182, 2192, 2211, 2214, 2229, 2460, 2531, 5930, 9511]
surviving nonsolo ucounts = 31[421, 557, 671, 915, 1003, 1047, 1063, 1266, 1275, 1429, 1570, 1605, 1616, 1666, 1851, 1874, 1884, 1896, 2029, 2115, 2151, 2162, 2182, 2192, 2211, 2214, 2229, 2460, 2531, 5930, 9511]
ids = [86, 977, 378, 1194, 251, 120, 990, 215, 1102, 775, ...]

====================================================================================

UMI info for barcode GTCAAGTTCCTTTCTC-1 contig 1 = ACAGTGATCC...
umi AAAACTCAGG = 1851 reads: +391 validated
umi ACCTGAATTC = 1057 reads: +391 validated
umi AGCAGTTTCG = 2151 reads: +391 validated
umi AGCCAATGTG = 1619 reads: +391 validated
umi AGGTTGGACA = 1240 reads: +391 validated
umi AGTAGGGCTC = 1608 reads: +391 validated
umi ATCAGACTCT = 1008 reads: +391 validated
umi ATGATATTGT = 2223 reads: +391 validated
umi CAGCTGCTGC = 8719 reads: -318X +1 -3XX +1 -7XX +1 -11XX +3 -7XX +39 invalidated
umi CATGTCCAGG = 673 reads: +391 validated
umi CTAGTGTATC = 1887 reads: +391 validated
umi CTCAATTCGA = 2195 reads: +391 validated
umi CTTGAGGATT = 2219 reads: +391 validated
umi CTTTACTCCT = 1906 reads: +391 validated
umi CTTTGGCATG = 2457 reads: +391 validated
umi GCACTACCTC = 1549 reads: +391 validated
umi GGCAAACCAG = 1450 reads: +391 validated
umi TATCGCTACG = 515 reads: -318X +1 -3X +1 -7XX +1 -11XX +3 -7XX +39 invalidated
umi TATTTCGTGG = 1055 reads: +391 validated
umi TCATCCCCCT = 2553 reads: +391 validated
umi TCCCGTTGGC = 1735 reads: +227 -1XX +163 invalidated
umi TCGAACGTCA = 5455 reads: -318X +1 -3XX +1 -7XX +1 -11XX +3 -7XX +39 invalidated
umi TGCTGCTTCG = 1893 reads: +391 validated
umi TTAAGTCCTC = 2023 reads: +391 validated
umi TTAGCACGGG = 916 reads: +391 validated
umi TTCAAACGCA = 2119 reads: +391 validated
umi TTGCTCCACC = 2184 reads: +391 validated
umi TTTGTGTCCT = 2232 reads: +391 validated

UMI info for barcode GTCAAGTTCCTTTCTC-1 contig 2 = AGTCAACTTC...
umi ACAATGCTAG = 2176 reads: +409 validated

UMI info for barcode GTCAAGTTCCTTTCTC-1 contig 3 = ATATGTAAAA...
umi AATTTTGCGC = 437 reads: +185 -1X +226 invalidated
umi TCTGACCGCT = 1273 reads: +412 validated

GOOD CONTIGS

TIG 1[bases=490]
0-47 ==> 0-47 on |674|TRBV7-2|5'UTR| [len=47] (mis=0)
47-386 ==> 0-339 on |675|TRBV7-2|L-REGION+V-REGION| [len=347] (mis=0)
399-438 ==> 10-49 on |546|TRBJ2-3|J-REGION| [len=49] (mis=0)
438-490 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 25 umis using 2643 reads
cdr3 = CASGSGTSQYF at 377, score = 8 + 8
umis assigned: [5, 120, 181, 183, 215, 221, 251, 280, 359, 378] and 18 others
of which 28 are surviving nonsolos
reads assigned: 57507
start codons at 47, 156
confident = true

TIG 2[bases=487]
0-36 ==> 0-36 on |455|TRAV10|5'UTR| [len=36] (mis=0)
36-379 ==> 0-343 on |456|TRAV10|L-REGION+V-REGION| [len=343] (mis=2)
379-445 ==> 0-66 on |440|TRAJ53|J-REGION| [len=66] (mis=3)
445-487 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 1 umis using 121 reads
cdr3 = CVVSPYSGGSNYKLTF at 366, score = 6 + 9
umis assigned: [88]
of which 1 are surviving nonsolos
reads assigned: 2130
start codons at 36, 94, 164, 246, 298
confident = true

TIG 3[bases=565]
0-111 ==> 0-111 on |494|TRAV29/DV5|5'UTR| [len=111] (mis=0)
111-469 ==> 0-358 on |495|TRAV29/DV5|L-REGION+V-REGION| [len=358] (mis=3)
468-523 ==> 11-66 on |429|TRAJ42|J-REGION| [len=66] (mis=0)
523-565 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 2 umis using 57 reads
cdr3 = CAARGSQGNLIF at 456, score = 9 + 10
umis assigned: [86, 1102]
of which 2 are surviving nonsolos
reads assigned: 1667
start codons at 2, 9, 30, 111, 117, 184, 187, 273, 290, 358, 364
confident = true
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.41 = GTCAAGTTCGGACAAG-1

using 6446 reads

====================================================================================

graph has 3698 edges initially, 96 edges after simplification

total ucounts = 487
nonsolo ucounts = 389[2^57, 3^35, 4^35, 5^29, 6^24, 7^20, 8^14, 9^17, 10^13, 11^10, 12^14, 13^8, 14^6, 15^6, 16^8, 17, 18^5, 19^8, 20^5, 21^5, 22^5, 23^4, 24^6, 25^2, 26^3, 27^2, 28^3, 29^3, 30^4, 31^4, 32^2, 34^4, 35^2, 36^3, 37^2, 40^3, 47, 48, 49^2, 50, 52, 61, 68, 77, 81, 84, 105, 108, 113, 115, 121, 1273]
surviving nonsolo ucounts = 1[1273]
ids = [313]

====================================================================================

UMI info for barcode GTCAAGTTCGGACAAG-1 contig 1 = TGGGGAAGGA...
umi GGGGTATTCG = 1146 reads: +403 validated

GOOD CONTIGS

TIG 1[bases=540]
124-465 ==> 0-341 on |522|TRAV8-3|L-REGION+V-REGION| [len=341] (mis=0)
470-527 ==> 6-63 on |431|TRAJ44|J-REGION| [len=63] (mis=1)
junction support: 1 umis using 36 reads
cdr3 = CAVGARAGTASKLTF at 451, score = 7 + 9
umis assigned: [313]
of which 1 are surviving nonsolos
reads assigned: 1131
start codons at 124, 246, 260, 287, 434, 520
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.43 = GTCAAGTTCGGCATCG-1

using 39 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[5, 33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.55 = GTCAAGTTCTCAAGTG-1

using 85 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[85]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.57 = GTCAAGTTCTCGCTTG-1

using 80000 reads

====================================================================================

graph has 25754 edges initially, 359 edges after simplification

total ucounts = 1817
nonsolo ucounts = 1375[2^195, 3^132, 4^110, 5^109, 6^90, 7^87, 8^65, 9^55, 10^54, 11^45, 12^47, 13^40, 14^32, 15^29, 16^23, 17^16, 18^20, 19^16, 20^19, 21^14, 22^11, 23^9, 24^13, 25^6, 26^7, 27^10, 28^7, 29^6, 30^5, 31^7, 32, 33^3, 34^2, 35^4, 36^4, 39^2, 40^2, 41, 42^2, 44^2, 45^2, 46^2, 47, 48, 52^2, 56, 58, 59, 65, 71, 86, 87, 88, 97, 99, 111, 136, 145, 153, 177, 271, 314, 341, 376, 432, 496, 532, 547, 591, 701, 718, 769, 786, 807, 826, 858, 962, 964, 997, 1045, 1046, 1089, 1116, 1128, 1174, 1252, 1270, 1364, 1394, 1398, 1480, 1554, 1560, 1581, 1612, 1636, 1699, 1749^2, 1781, 1827, 1854, 1862, 1916, 1917, 1998, 2084, 2087, 2273, 6035]
surviving nonsolo ucounts = 50[177, 271, 341, 376, 432, 496, 532, 547, 591, 701, 718, 769, 786, 807, 826, 858, 962, 964, 997, 1045, 1046, 1089, 1116, 1128, 1174, 1252, 1270, 1364, 1394, 1398, 1480, 1554, 1560, 1581, 1612, 1636, 1699, 1749^2, 1781, 1827, 1854, 1862, 1916, 1917, 1998, 2084, 2087, 2273, 6035]
ids = [1048, 871, 994, 837, 1039, 1327, 522, 84, 18, 188, ...]

====================================================================================

UMI info for barcode GTCAAGTTCTCGCTTG-1 contig 1 = TGGGGGACTC...
umi ACGCGGCGGC = 689 reads: +389 -4XX +8 -1XX +1 invalidated
umi AGTGTATAGG = 718 reads: +403 validated
umi ATCTGTTAGT = 1118 reads: +403 validated
umi CCAGTTTTCA = 1554 reads: +403 validated
umi CGGCCTAGTT = 2048 reads: +403 validated
umi CTGAACGTTC = 1899 reads: +403 validated
umi CTTCGTGAGC = 376 reads: +403 validated
umi GAACGCCGGG = 261 reads: +403 validated
umi GGAGGGCCAC = 995 reads: +403 validated
umi GTCTCGTTTT = 743 reads: +403 validated
umi GTGCTTGGCA = 1093 reads: +403 validated
umi TATAGCTCCT = 1892 reads: +403 validated
umi TCGTTGCAGT = 1601 reads: +403 validated

UMI info for barcode GTCAAGTTCTCGCTTG-1 contig 2 = ATGTTCACAG...
umi AAACACACCG = 1392 reads: +397 validated
umi AAATAAGCTT = 592 reads: +397 validated
umi AACCAACAAT = 158 reads: +49 -3XX +1 -2XX +1 -4XX +1 -6XX +1 -7XX +3 -2XX +1 -1XX +2 -313X invalidated
umi AACGTGTCTT = 1828 reads: +397 validated
umi AATATCCTCC = 495 reads: -359 +1 -12X +1 -1XX +1 -3XX +2 -1XX +1 -2XX +2 -2XX +2 -1XX +1 -1XX +1 -1XX +2 invalidated
umi ACGGGTAGTT = 2091 reads: +397 validated
umi ACTTTTAGCA = 1697 reads: +397 validated
umi AGCTTGGTCT = 1366 reads: +397 validated
umi AGGCTCGTGT = 1782 reads: +397 validated
umi ATAACGGGAA = 1848 reads: +397 validated
umi ATTCTTTCCA = 1862 reads: +397 validated
umi CATCGTCGTC = 493 reads: -372 +1 -1X +1 -3X +2 -1XX +1 -2XX +2 -2XX +2 -1XX +1 -1XX +1 -1XX +2 invalidated
umi CCCTCACACA = 1276 reads: +397 validated
umi CTAAGCCGCG = 1759 reads: +397 validated
umi CTACCTCCTT = 1472 reads: +397 validated
umi CTCAACGAGG = 1003 reads: +397 validated
umi CTTCCTAGCA = 1414 reads: +397 validated
umi GATCATACAG = 2293 reads: +397 validated
umi GCGGTCTGCC = 824 reads: +397 validated
umi GGGAACGATA = 207 reads: +49 -3XX +1 -2XX +1 -4XX +1 -6XX +1 -7XX +3 -2XX +1 -1XX +2 -313XX invalidated
umi TAGGTCCACG = 497 reads: +397 validated
umi TATCAGTATG = 1570 reads: +397 validated
umi TCACTGTCGC = 1052 reads: +397 validated
umi TCTCTATTGT = 1750 reads: +397 validated
umi TCTGTTCTCC = 1632 reads: +397 validated
umi TGGCTATAGG = 2084 reads: +100 -1XX +296 invalidated
umi TGGTTTACGG = 1044 reads: +397 validated
umi TTATATATCT = 1086 reads: +397 validated
umi TTTAGCACTT = 1587 reads: +397 validated

GOOD CONTIGS

TIG 1[bases=475]
30-380 ==> 0-350 on |505|TRAV38-1|L-REGION+V-REGION| [len=350] (mis=5)
376-433 ==> 0-57 on |417|TRAJ31|J-REGION| [len=57] (mis=3)
433-475 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 13 umis using 956 reads
cdr3 = CAFIRGNNARLMF at 363, score = 7 + 7
umis assigned: [188, 317, 377, 571, 698, 794, 837, 871, 1046, 1170] and 3 others
of which 13 are surviving nonsolos
reads assigned: 14757
start codons at 30, 87, 120, 163, 222, 262, 354, 385, 396, 406
confident = true

TIG 2[bases=501]
0-52 ==> 7-59 on |567|TRBV12-4|5'UTR| [len=59] (mis=0)
52-392 ==> 0-340 on |568|TRBV12-4|L-REGION+V-REGION| [len=347] (mis=0)
399-449 ==> 0-50 on |540|TRBJ1-5|J-REGION| [len=50] (mis=4)
449-501 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 25 umis using 2501 reads
cdr3 = CASTKLKDQPQHF at 382, score = 8 + 8
umis assigned: [6, 18, 32, 44, 84, 190, 243, 277, 285, 332] and 19 others
of which 29 are surviving nonsolos
reads assigned: 37489
start codons at 0, 52, 110, 151, 174, 223, 226, 275, 286, 313, 320, 425
confident = true

REJECT CONTIGS

TIG 1[bases=321]
5-219 ==> 3012-3226 on segment before TRBJ2-1 exon 1 [len=3226] (mis=0)
219-269 ==> 0-50 on |543|TRBJ2-1|J-REGION| [len=50] (mis=0)
269-321 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
umis assigned: [75, 253, 994, 1048]
of which 3 are surviving nonsolos
reads assigned: 6408
start codons at 78, 227
confident = false
did not find CDR3

TIG 2[bases=581]
0-111 ==> 0-111 on |494|TRAV29/DV5|5'UTR| [len=111] (mis=1)
111-469 ==> 0-358 on |495|TRAV29/DV5|L-REGION+V-REGION| [len=358] (mis=1)
470-539 ==> 0-69 on |439|TRAJ52|J-REGION| [len=69] (mis=3)
539-581 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
cdr3 = CTSVQQAQTAGGTSYGKLTF at 448, score = 3 + 9
umis assigned: [548, 1039, 1334, 1392, 1419]
of which 5 are surviving nonsolos
reads assigned: 3755
start codons at 2, 9, 30, 111, 117, 184, 187, 273, 290, 358, 364, 491
confident = false
frameshifted full length stopped transcript of length 581
VJ delta = 39
delta too large
not full
not full
now this is a cell
paired!

TCACAGCTGGGGGACACTGCGATGTATTTCTGTGCTTTCATCCGGGGGAACAATGCCAGACTCATGTTTGGAGATGGAACTCAGCTGGTGGTGAAGCCCA <==> CCCTCAGAACCCAGGGACTCAGCTGTGTACTTCTGTGCCAGCACCAAATTGAAGGATCAGCCCCAGCATTTTGGTGATGGGACTCGACTCTCCATCCTAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.63 = GTCAAGTTCTGTCAAG-1

using 332 reads

====================================================================================

graph has 198 edges initially, 10 edges after simplification

total ucounts = 14
nonsolo ucounts = 14[2, 3^2, 4, 7^2, 10^2, 20, 22, 33, 35, 84, 92]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.66 = GTCAAGTTCTTCAACT-1

using 32 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 1[30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.67 = GTCAAGTTCTTCCTTC-1

using 26497 reads

====================================================================================

graph has 12078 edges initially, 342 edges after simplification

total ucounts = 1079
nonsolo ucounts = 830[2^111, 3^89, 4^57, 5^55, 6^31, 7^38, 8^39, 9^31, 10^21, 11^31, 12^31, 13^26, 14^21, 15^17, 16^17, 17^23, 18^9, 19^12, 20^12, 21^14, 22^13, 23^15, 24^12, 25^11, 26^10, 27^5, 28^7, 29^7, 30^9, 31^5, 32^3, 33^4, 34^3, 35^5, 36^3, 37^2, 38^2, 39^2, 40^2, 42^3, 44^3, 47^2, 57, 66, 75, 81, 86, 116, 233, 1025, 1189, 1253, 1384, 1452, 1606, 1744, 2121, 2289, 2353]
surviving nonsolo ucounts = 11[233, 1025, 1189, 1253, 1384, 1452, 1606, 1744, 2121, 2289, 2353]
ids = [381, 831, 868, 866, 97, 31, 948, 105, 631, 992, ...]

====================================================================================

UMI info for barcode GTCAAGTTCTTCCTTC-1 contig 1 = GAGAGTCCTG...
umi AAATCATTGG = 1 reads: -397X invalidated
umi AACTGCCTCT = 1442 reads: +392 -2XX +3 invalidated
umi ACCATGTCCT = 1303 reads: -361X +1 -15XX +1 -3XX +3 -5XX +1 -5XX +2 invalidated
umi ACCGCTTCGT = 1740 reads: +397 validated
umi ATTGCATCGT = 2356 reads: +397 validated
umi CGATAGTCCG = 212 reads: -377 +1 -3X +3 -5XX +1 -5XX +2 invalidated
umi GGTATATTTA = 2126 reads: +397 validated
umi TCTATCGCCA = 951 reads: -361X +1 -15XX +1 -3XX +3 -5XX +1 -5XX +2 invalidated
umi TGACAGATCA = 1163 reads: -361X +1 -15XX +1 -3XX +3 -5XX +1 -5XX +2 invalidated
umi TGACCTCAAT = 1189 reads: +397 validated
umi TGTTCTGCCT = 1620 reads: +397 validated

GOOD CONTIGS

TIG 1[bases=515]
0-66 ==> 0-66 on |657|TRBV6-5|5'UTR| [len=66] (mis=0)
66-410 ==> 0-344 on |658|TRBV6-5|L-REGION+V-REGION| [len=344] (mis=1)
416-463 ==> 0-47 on |550|TRBJ2-7|J-REGION| [len=47] (mis=5)
463-515 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 6 umis using 717 reads
cdr3 = CASSYYGDGLEQYF at 393, score = 9 + 8
umis assigned: [16, 31, 97, 105, 252, 381, 631, 831, 866, 868] and 1 others
of which 10 are surviving nonsolos
reads assigned: 13918
start codons at 24, 52, 66, 124, 177, 201, 208, 216, 243, 307, 316
confident = true

REJECT CONTIGS

TIG 1[bases=464]
0-23 ==> 0-23 on |463|TRAV13-1|5'UTR| [len=23] (mis=0)
23-360 ==> 0-337 on |464|TRAV13-1|L-REGION+V-REGION| [len=337] (mis=5)
359-422 ==> 0-63 on |443|TRAJ57|J-REGION| [len=63] (mis=3)
422-464 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
cdr3 = CSPTPQGGSEKLVF at 349, score = 3 + 9
umis assigned: [992]
of which 1 are surviving nonsolos
reads assigned: 2264
start codons at 23, 78, 87, 237
confident = false
frameshifted full length stopped transcript of length 464
VJ delta = 23
not full
not full
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.70 = GTCAAGTTCTTTAGGG-1

using 21 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.76 = GTCACAAAGACCGGAT-1

using 129 reads

====================================================================================

graph has 68 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 5[4^2, 5, 16, 98]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.77 = GTCACAAAGACGCAAC-1

using 1955 reads

====================================================================================

graph has 648 edges initially, 2 edges after simplification

total ucounts = 12
nonsolo ucounts = 9[2^3, 4^2, 9^2, 13, 1907]
surviving nonsolo ucounts = 1[1907]
ids = [10]

====================================================================================

UMI info for barcode GTCACAAAGACGCAAC-1 contig 1 = GGGGTCACAG...
umi TGAATACTTC = 1904 reads: +397 validated

GOOD CONTIGS

TIG 1[bases=502]
53-400 ==> 0-347 on |688|TRBV7-9|L-REGION+V-REGION| [len=347] (mis=2)
400-450 ==> 0-50 on |543|TRBJ2-1|J-REGION| [len=50] (mis=3)
450-502 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 1 umis using 128 reads
cdr3 = CASSLGANNEQFF at 383, score = 7 + 8
umis assigned: [10]
of which 1 are surviving nonsolos
reads assigned: 1876
start codons at 53, 77, 162, 261, 374, 408
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.78 = GTCACAAAGAGCTGCA-1

using 4830 reads

====================================================================================

graph has 4158 edges initially, 138 edges after simplification

total ucounts = 386
nonsolo ucounts = 308[2^39, 3^36, 4^19, 5^21, 6^16, 7^17, 8^14, 9^12, 10^7, 11^7, 12^14, 13^13, 14^6, 15^7, 16^4, 17^4, 18^4, 19^4, 20^4, 21^4, 22^5, 23^5, 24^4, 26, 27, 28, 29^3, 31^2, 32^2, 34^2, 36, 40^2, 41^3, 43, 45^2, 46^2, 47, 49^3, 52, 56, 57, 58, 67, 70, 71, 76, 78, 79, 80, 87, 92, 95, 474]
surviving nonsolo ucounts = 1[474]
ids = [27]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.79 = GTCACAAAGAGTGACC-1

using 58 reads

====================================================================================

graph has 82 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 4[8, 11, 12, 27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.80 = GTCACAAAGATAGGAG-1

using 93 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[3, 90]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.84 = GTCACAAAGCCACCTG-1

using 94 reads

====================================================================================

graph has 56 edges initially, 4 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[22, 72]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.87 = GTCACAAAGCGCTTAT-1

using 38 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.89 = GTCACAAAGCGTGTCC-1

using 51 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[51]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.92 = GTCACAAAGCTGGAAC-1

using 32 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.96 = GTCACAAAGGATGTAT-1

using 53 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[53]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.99 = GTCACAAAGGGTTTCT-1

using 18 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.100 = GTCACAAAGGTAAACT-1

using 33298 reads

====================================================================================

graph has 19328 edges initially, 644 edges after simplification

total ucounts = 1318
nonsolo ucounts = 1128[2^93, 3^65, 4^52, 5^61, 6^55, 7^37, 8^41, 9^39, 10^29, 11^29, 12^21, 13^22, 14^25, 15^19, 16^19, 17^24, 18^10, 19^24, 20^11, 21^6, 22^8, 23^12, 24^8, 25^12, 26^11, 27^7, 28^5, 29^7, 30^4, 31^3, 32^16, 33^10, 34^4, 35^10, 36^5, 37^5, 38^4, 39^4, 40^5, 41^10, 42^6, 43^8, 44^5, 45^6, 46^7, 47^3, 48^11, 49^6, 50^4, 51^4, 52^9, 53^3, 54^3, 55^5, 57^5, 58^3, 59^6, 60^6, 61^2, 62^5, 63^5, 64^2, 65^9, 66^3, 67^6, 68^2, 69^2, 70^4, 71^3, 73^3, 74^3, 75^7, 76^6, 77^6, 78^4, 79, 80^2, 81^6, 82^2, 83^5, 84, 85^4, 86^4, 87^4, 88^4, 89^5, 90^4, 91, 92^4, 93, 94^4, 95^4, 96, 97^5, 98^6, 99^3, 100^2, 101^7, 102^2, 104^2, 105, 106^4, 107, 108, 109^3, 110, 111, 112^3, 113, 114^2, 115^2, 116, 119, 120, 121, 123^3, 124^3, 128^3, 129, 135, 146, 150, 151, 152, 516]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.102 = GTCACAAAGGTGGGTT-1

using 17 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[17]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.103 = GTCACAAAGTACACCT-1

using 126 reads

====================================================================================

graph has 58 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[2, 122]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.109 = GTCACAAAGTCCCACG-1

using 877 reads

====================================================================================

graph has 458 edges initially, 12 edges after simplification

total ucounts = 36
nonsolo ucounts = 30[2^5, 3^2, 4^2, 5, 6, 9, 10^2, 11, 20, 22, 23, 24, 29, 47, 48, 50, 51, 55, 58, 69, 96, 100, 104]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.111 = GTCACAAAGTGAAGTT-1

using 183 reads

====================================================================================

graph has 96 edges initially, 4 edges after simplification

total ucounts = 13
nonsolo ucounts = 8[2^2, 6, 7, 9, 26, 29, 97]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.113 = GTCACAAAGTGCTGCC-1

using 61 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[61]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.118 = GTCACAAAGTGTTGAA-1

using 27 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.119 = GTCACAAAGTTCGCAT-1

using 21 reads

====================================================================================

graph has 6 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.120 = GTCACAACAAACTGCT-1

using 73 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[73]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.121 = GTCACAACAAATACAG-1

using 207 reads

====================================================================================

graph has 106 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 6[2, 5, 18, 20, 28, 132]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.131 = GTCACAACACAGAGGT-1

using 47 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[47]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.135 = GTCACAACACCGAATT-1

using 7088 reads

====================================================================================

graph has 6095 edges initially, 202 edges after simplification

total ucounts = 674
nonsolo ucounts = 523[2^59, 3^43, 4^39, 5^38, 6^22, 7^27, 8^26, 9^21, 10^17, 11^17, 12^18, 13^15, 14^21, 15^19, 16^18, 17^11, 18^6, 19^13, 20^6, 21^15, 22^5, 23^5, 24^4, 25^2, 26^7, 27^3, 28^3, 29^5, 30^5, 31^2, 32^3, 33, 34, 36, 37^2, 39^2, 40^2, 41^2, 42^2, 45, 48^2, 49, 51, 55, 58, 61, 63, 70, 72, 76, 98^2, 449]
surviving nonsolo ucounts = 1[449]
ids = [37]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.137 = GTCACAACACGCTTTC-1

using 100 reads

====================================================================================

graph has 98 edges initially, 4 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[9, 23, 29, 37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.138 = GTCACAACACGGTGTC-1

using 28 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.139 = GTCACAACACTAGTAC-1

using 15 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.143 = GTCACAACAGATCTGT-1

using 116 reads

====================================================================================

graph has 44 edges initially, 4 edges after simplification

total ucounts = 3
nonsolo ucounts = 3[4, 42, 70]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.144 = GTCACAACAGATGGCA-1

using 20 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.148 = GTCACAACAGCTATTG-1

using 20 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.150 = GTCACAACATACCATG-1

using 57 reads

====================================================================================

graph has 72 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 4[5, 8, 15, 29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.163 = GTCACAACATTTGCTT-1

using 62 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[61]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.164 = GTCACAAGTAACGCGA-1

using 61 reads

====================================================================================

graph has 68 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 4[5, 6, 24, 25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.165 = GTCACAAGTAAGCACG-1

using 24 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.166 = GTCACAAGTACCATCA-1

using 61 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[61]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.172 = GTCACAAGTCTAGGTT-1

using 370 reads

====================================================================================

graph has 260 edges initially, 6 edges after simplification

total ucounts = 20
nonsolo ucounts = 17[2, 3^3, 5, 6, 7, 8, 9, 12, 13, 14, 34, 38, 44, 46, 120]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.176 = GTCACAAGTGAGGGTT-1

using 77 reads

====================================================================================

graph has 64 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 4[3, 17, 24, 32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.177 = GTCACAAGTGCAACTT-1

using 146 reads

====================================================================================

graph has 134 edges initially, 6 edges after simplification

total ucounts = 10
nonsolo ucounts = 8[3^2, 9, 10, 11, 28, 30, 50]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.179 = GTCACAAGTGGCTCCA-1

using 91 reads

====================================================================================

graph has 92 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 7[2, 5, 6, 7, 8, 30, 33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.180 = GTCACAAGTTACCAGT-1

using 161 reads

====================================================================================

graph has 199 edges initially, 9 edges after simplification

total ucounts = 15
nonsolo ucounts = 14[2^3, 3^3, 4^3, 18, 20, 23, 24, 48]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.182 = GTCACAAGTTACGGAG-1

using 28 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 1[18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.186 = GTCACAAGTTGGTAAA-1

using 47 reads

====================================================================================

graph has 64 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[2, 15, 28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.193 = GTCACAATCAGCATGT-1

using 144 reads

====================================================================================

graph has 182 edges initially, 6 edges after simplification

total ucounts = 7
nonsolo ucounts = 7[4, 8, 16, 17, 21, 28, 50]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.194 = GTCACAATCAGGCAAG-1

using 69 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[69]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.200 = GTCACAATCCAGAGGA-1

using 117 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[117]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.203 = GTCACAATCCGCAGTG-1

using 78 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[78]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.205 = GTCACAATCCGTAGGC-1

using 52 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[52]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.206 = GTCACAATCCGTCAAA-1

using 64 reads

====================================================================================

graph has 80 edges initially, 4 edges after simplification

total ucounts = 14
nonsolo ucounts = 7[2^2, 3, 4^2, 18, 24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.207 = GTCACAATCCTACAGA-1

using 24 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.209 = GTCACAATCCTCCTAG-1

using 46 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 1[43]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.215 = GTCACAATCGGACAAG-1

using 23361 reads

====================================================================================

graph has 16547 edges initially, 648 edges after simplification

total ucounts = 1447
nonsolo ucounts = 1152[2^125, 3^107, 4^66, 5^69, 6^52, 7^47, 8^68, 9^46, 10^51, 11^40, 12^41, 13^34, 14^29, 15^38, 16^23, 17^19, 18^25, 19^17, 20^18, 21^12, 22^16, 23^13, 24^7, 25^6, 26^21, 27^13, 28^8, 29^4, 30^7, 31^5, 32^6, 33^6, 34^6, 35^6, 36^3, 37^2, 38^3, 39^5, 40^4, 41^2, 42^2, 43^2, 45^4, 46^3, 47^2, 48^4, 49^3, 50^2, 52, 53^3, 54^5, 55^4, 57, 58, 59, 61, 62, 63^5, 64, 65, 66, 67^2, 68^3, 69^2, 71, 72^2, 73, 75, 80, 83, 84^3, 86, 87, 88, 89, 91, 93, 99, 101, 102, 103, 104, 107, 110, 117, 129, 961, 4993]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.220 = GTCACAATCTCGATGA-1

using 3838 reads

====================================================================================

graph has 3414 edges initially, 106 edges after simplification

total ucounts = 479
nonsolo ucounts = 350[2^55, 3^44, 4^30, 5^25, 6^25, 7^16, 8^15, 9^13, 10^18, 11^10, 12^6, 13^4, 14^6, 15^3, 16^9, 17^8, 18^7, 19^4, 20^5, 21^2, 22^4, 23, 24^3, 25^2, 26^3, 27^2, 29^4, 30^2, 31^2, 32^4, 33, 34, 36, 37^2, 39^3, 40, 41, 43^2, 45^2, 50, 72, 76^2]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.221 = GTCACAATCTCTGCTG-1

using 82 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[81]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.222 = GTCACAATCTCTTGAT-1

using 43 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[43]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.223 = GTCACAATCTGACCTC-1

using 98 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[98]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.225 = GTCACAATCTGCTTGC-1

using 94 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[94]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.231 = GTCACAATCTTGAGGT-1

using 530 reads

====================================================================================

graph has 360 edges initially, 4 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[45, 483]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.236 = GTCACGGAGAAGGTGA-1

using 40 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[39]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.239 = GTCACGGAGACGCAAC-1

using 4591 reads

====================================================================================

graph has 4180 edges initially, 114 edges after simplification

total ucounts = 510
nonsolo ucounts = 389[2^47, 3^38, 4^31, 5^26, 6^19, 7^22, 8^17, 9^20, 10^13, 11^12, 12^20, 13^11, 14^15, 15^13, 16^11, 17^9, 18^3, 19^5, 20^3, 21^5, 22^5, 23^2, 24^3, 25, 26^3, 27^5, 28^3, 29^2, 31, 32^2, 33^2, 34, 37^2, 38, 40^2, 43, 46, 47^2, 48^2, 49, 51^2, 53^2, 63^2, 73]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.246 = GTCACGGAGATCTGAA-1

using 88 reads

====================================================================================

graph has 57 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[3, 83]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.252 = GTCACGGAGCTGATAA-1

using 29 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.254 = GTCACGGAGCTGCGAA-1

using 63 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 1[60]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.256 = GTCACGGAGGCCCTTG-1

using 37 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 1[33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.257 = GTCACGGAGGCCGAAT-1

using 49 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[2, 22, 24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.259 = GTCACGGAGGCTAGGT-1

using 41 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[14, 26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.262 = GTCACGGAGGGATGGG-1

using 65 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[65]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.265 = GTCACGGAGGTGATTA-1

using 37 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 4[4, 6, 7, 19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.272 = GTCACGGAGTCTCAAC-1

using 690 reads

====================================================================================

graph has 316 edges initially, 4 edges after simplification

total ucounts = 8
nonsolo ucounts = 6[2, 5, 11, 18, 21, 631]
surviving nonsolo ucounts = 1[631]
ids = [7]

====================================================================================

UMI info for barcode GTCACGGAGTCTCAAC-1 contig 1 = GGGGACTCTT...
umi TTGAATTAGT = 630 reads: +379 validated

GOOD CONTIGS

TIG 1[bases=518]
0-97 ==> 0-97 on |492|TRAV27|5'UTR| [len=97] (mis=3)
97-425 ==> 0-328 on |493|TRAV27|L-REGION+V-REGION| [len=328] (mis=2)
426-476 ==> 7-57 on |405|TRAJ20|J-REGION| [len=57] (mis=0)
476-518 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 1 umis using 39 reads
cdr3 = CAGFDYKLSF at 415, score = 10 + 8
umis assigned: [7]
of which 1 are surviving nonsolos
reads assigned: 620
start codons at 70, 79, 97, 141, 249, 350
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.278 = GTCACGGCAAACTGCT-1

using 76 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[76]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.282 = GTCACGGCAATAACGA-1

using 23 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[3^2, 16]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.283 = GTCACGGCAATAGAGT-1

using 64 reads

====================================================================================

graph has 105 edges initially, 4 edges after simplification

total ucounts = 3
nonsolo ucounts = 3[2, 24, 38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.286 = GTCACGGCAATGGAAT-1

using 37 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.287 = GTCACGGCACAACTGT-1

using 63 reads

====================================================================================

graph has 52 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[4, 10, 20, 27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.288 = GTCACGGCACAGACTT-1

using 50 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[50]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.289 = GTCACGGCACAGAGGT-1

using 40695 reads

====================================================================================

graph has 15294 edges initially, 178 edges after simplification

total ucounts = 1059
nonsolo ucounts = 765[2^129, 3^72, 4^71, 5^71, 6^45, 7^38, 8^49, 9^40, 10^28, 11^28, 12^18, 13^18, 14^17, 15^16, 16^15, 17^12, 18^7, 19^11, 20^3, 21^4, 22^8, 24^5, 25, 26^3, 27^3, 28^3, 29, 30, 31^2, 34, 35, 36^2, 38, 39, 41, 42, 50, 54, 56^2, 57, 64, 67^3, 73, 75, 81, 85, 100, 110, 122, 338, 375, 403, 503, 829, 866, 913, 1066, 1412, 1523, 1581, 1603, 1614, 1704, 1889, 1894, 2059, 2170, 2185, 2226, 2300, 3938]
surviving nonsolo ucounts = 21[338, 375, 403, 503, 866, 913, 1066, 1412, 1523, 1581, 1603, 1614, 1704, 1889, 1894, 2059, 2170, 2185, 2226, 2300, 3938]
ids = [356, 973, 500, 27, 466, 1032, 708, 771, 689, 138, ...]

====================================================================================

UMI info for barcode GTCACGGCACAGAGGT-1 contig 1 = CTCAGAGGAC...
umi AGATTTCCCT = 1591 reads: +397 validated
umi CAGAGTCTTG = 1596 reads: +397 validated
umi CCGGTGTCCG = 1899 reads: +397 validated
umi CGATACTAGT = 1715 reads: +397 validated
umi CGCTTCGTTC = 1907 reads: +397 validated
umi CTATCGCCGC = 2185 reads: +397 validated
umi CTCGTGGCGA = 861 reads: +387 -1 +1 -8XX invalidated
umi CTTGGTCTAT = 400 reads: +397 validated
umi GCCAAGACAA = 2346 reads: +229 -1XX +167 invalidated
umi GTCTATCTTC = 1522 reads: +397 validated
umi TAAAATGATC = 2167 reads: +397 validated
umi TAGCTATCGG = 1332 reads: -361X +1 -20XX +2 -11XX +2 invalidated
umi TGACAGGCTC = 2072 reads: +397 validated
umi TGTGCCAGCA = 4189 reads: +108 -4XX +1 -5XX +1 -3XX +2 -9XX +2 -14X +1 -2XX +1 -3XX +1 -9XX +1 -1XX +2 -3XX +224 invalidated
umi TTAAAAACGA = 2247 reads: +397 validated
umi TTATAGGGGC = 1624 reads: +397 validated
umi TTCAGTGTTC = 386 reads: +312 -2 +2 -76 +5 non-validated
umi TTTCATATCT = 920 reads: +347 -45 +5 non-validated

UMI info for barcode GTCACGGCACAGAGGT-1 contig 2 = GGACTTCTGT...
umi AACCCCTATC = 506 reads: +400 validated
umi CCGGCTTCAA = 343 reads: +400 validated
umi GTTCCACTCT = 1090 reads: +400 validated

GOOD CONTIGS

TIG 1[bases=758]
0-309 ==> 0-309 on |630|TRBV5-1|5'UTR| [len=309] (mis=0)
309-652 ==> 0-343 on |631|TRBV5-1|L-REGION+V-REGION| [len=343] (mis=3)
670-706 ==> 14-50 on |543|TRBJ2-1|J-REGION| [len=50] (mis=0)
706-758 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 15 umis using 1740 reads
cdr3 = CASSLSTSGEVKFF at 636, score = 9 + 8
umis assigned: [138, 284, 357, 396, 415, 453, 466, 500, 586, 689] and 8 others
of which 18 are surviving nonsolos
reads assigned: 30102
start codons at 58, 78, 210, 309, 591, 595
confident = true

TIG 2[bases=583]
141-481 ==> 0-340 on |514|TRAV5|L-REGION+V-REGION| [len=340] (mis=3)
479-541 ==> 0-62 on |423|TRAJ37|J-REGION| [len=62] (mis=4)
541-583 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 3 umis using 77 reads
cdr3 = CAEIHSGNTGKLIF at 468, score = 7 + 8
umis assigned: [27, 356, 708]
of which 3 are surviving nonsolos
reads assigned: 1895
start codons at 33, 141, 195, 211, 360, 366
confident = true
now this is a cell
paired!

CAGACTGGGGACTCAGCTATCTACTTCTGTGCAGAGATCCACTCTGGCAACACAGGCAAACTAATCTTTGGGCAAGGGACAACTTTACAAGTAAAACCAG <==> TTGGAGCTGGGGGACTCGGCCCTTTATCTTTGCGCCAGCAGCCTCTCGACTAGCGGTGAAGTGAAGTTCTTCGGGCCAGGGACACGGCTCACCGTGCTAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.292 = GTCACGGCACATTTCT-1

using 37 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.294 = GTCACGGCACGAAACG-1

using 78 reads

====================================================================================

graph has 62 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 5[3, 6, 7, 24, 38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.295 = GTCACGGCACGAGGTA-1

using 29 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.296 = GTCACGGCACGCCAGT-1

using 22 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.297 = GTCACGGCACGCTTTC-1

using 24 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.299 = GTCACGGCACGGTAAG-1

using 34 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.306 = GTCACGGCAGCTGCAC-1

using 388 reads

====================================================================================

graph has 234 edges initially, 6 edges after simplification

total ucounts = 14
nonsolo ucounts = 10[2, 3^2, 4, 7, 12, 31, 102, 106, 114]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.310 = GTCACGGCAGTAAGAT-1

using 48 reads

====================================================================================

graph has 64 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 3[2, 15, 27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.313 = GTCACGGCATACTCTT-1

using 167 reads

====================================================================================

graph has 138 edges initially, 4 edges after simplification

total ucounts = 13
nonsolo ucounts = 12[2^2, 3^4, 5, 6, 8, 23, 33, 75]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.314 = GTCACGGCATATGAGA-1

using 96 reads

====================================================================================

graph has 115 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 7[2, 3^2, 5, 7, 22, 54]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.315 = GTCACGGCATCACGAT-1

using 118 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[118]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.316 = GTCACGGCATCAGTCA-1

using 38 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 5[3, 5, 7, 10^2]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.319 = GTCACGGCATGAACCT-1

using 15 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 2[2, 9]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.320 = GTCACGGCATGCAATC-1

using 20 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.324 = GTCACGGCATTTGCCC-1

using 30 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.329 = GTCACGGGTAATCGTC-1

using 15 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.334 = GTCACGGGTAGGGTAC-1

using 902 reads

====================================================================================

graph has 372 edges initially, 4 edges after simplification

total ucounts = 10
nonsolo ucounts = 6[2^2, 6, 11, 24, 853]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.336 = GTCACGGGTATGAAAC-1

using 83 reads

====================================================================================

graph has 112 edges initially, 2 edges after simplification

total ucounts = 13
nonsolo ucounts = 9[2, 3, 6, 7, 8, 10, 11, 14, 18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.343 = GTCACGGGTCCGTCAG-1

using 23 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.347 = GTCACGGGTCTGCCAG-1

using 12 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[12]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.356 = GTCACGGGTGCAGACA-1

using 167 reads

====================================================================================

graph has 56 edges initially, 4 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[76, 90]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.357 = GTCACGGGTGCGAAAC-1

using 106 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[106]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.359 = GTCACGGGTGGTAACG-1

using 93 reads

====================================================================================

graph has 110 edges initially, 2 edges after simplification

total ucounts = 18
nonsolo ucounts = 11[2^4, 3, 4^3, 11, 23, 29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.361 = GTCACGGGTGTAACGG-1

using 3498 reads

====================================================================================

graph has 3133 edges initially, 70 edges after simplification

total ucounts = 411
nonsolo ucounts = 301[2^52, 3^46, 4^31, 5^21, 6^26, 7^17, 8^14, 9^15, 10^9, 11^7, 12^6, 13^2, 14^7, 15^3, 16^6, 17^2, 18^3, 19^5, 20^2, 21^2, 22, 23^2, 24^3, 25, 26^2, 27^2, 28, 32, 34, 35, 40, 45, 48, 50, 58, 62, 63, 84, 97, 679]
surviving nonsolo ucounts = 1[679]
ids = [335]

====================================================================================

REJECT CONTIGS

TIG 1[bases=561]
4-224 ==> 325-545 on segment before TRBJ1-4 exon 1 [len=545] (mis=0)
224-275 ==> 0-51 on |539|TRBJ1-4|J-REGION| [len=51] (mis=0)
umis assigned: [335]
of which 1 are surviving nonsolos
reads assigned: 653
start codons at 216, 230, 276, 320, 381, 463, 479, 538
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.369 = GTCACGGGTTAAGAAC-1

using 40 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[39]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.370 = GTCACGGGTTAAGTAG-1

using 113 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[113]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.372 = GTCACGGGTTACGGAG-1

using 12 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[12]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.374 = GTCACGGGTTAGAACA-1

using 38 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 3[3, 12, 20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.378 = GTCACGGGTTCCACTC-1

using 117 reads

====================================================================================

graph has 84 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 1[112]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.385 = GTCACGGGTTGGTTTG-1

using 613 reads

====================================================================================

graph has 614 edges initially, 26 edges after simplification

total ucounts = 123
nonsolo ucounts = 76[2^21, 3^7, 4^10, 5^4, 6^10, 7^3, 8^4, 9^3, 10^2, 15^3, 16, 19, 20, 21, 24, 26, 27, 36, 49]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.387 = GTCACGGTCAACACAC-1

using 77 reads

====================================================================================

graph has 88 edges initially, 2 edges after simplification

total ucounts = 21
nonsolo ucounts = 13[2^6, 3^2, 5, 6, 7, 15, 18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.389 = GTCACGGTCAACGGGA-1

using 75 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[75]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.392 = GTCACGGTCAAGGCTT-1

using 52 reads

====================================================================================

graph has 40 edges initially, 4 edges after simplification

total ucounts = 7
nonsolo ucounts = 3[2, 15, 31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.393 = GTCACGGTCACCACCT-1

using 46 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[46]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.397 = GTCACGGTCACGGTTA-1

using 48 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[48]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.402 = GTCACGGTCAGTGTTG-1

using 31 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.403 = GTCACGGTCATAGCAC-1

using 49 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 5[2, 4^2, 13, 26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.405 = GTCACGGTCATCTGTT-1

using 30 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.406 = GTCACGGTCATGCAAC-1

using 51 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[51]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.407 = GTCACGGTCCACTGGG-1

using 55 reads

====================================================================================

graph has 43 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[55]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.412 = GTCACGGTCCTCCTAG-1

using 131 reads

====================================================================================

graph has 68 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[131]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.413 = GTCACGGTCCTCGCAT-1

using 72 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[72]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.414 = GTCACGGTCCTTTACA-1

using 26540 reads

====================================================================================

graph has 15911 edges initially, 365 edges after simplification

total ucounts = 2052
nonsolo ucounts = 1495[2^281, 3^178, 4^144, 5^114, 6^112, 7^91, 8^81, 9^63, 10^53, 11^54, 12^44, 13^26, 14^21, 15^17, 16^30, 17^21, 18^17, 19^20, 20^16, 21^6, 22^9, 23^11, 24^7, 25^6, 26^3, 27^3, 28^5, 29, 30^4, 31^7, 32, 33^4, 34^3, 36^2, 37^5, 38^2, 39, 40, 41^2, 44^2, 46^2, 47^5, 48, 49^2, 50, 51, 52, 53, 54^3, 57, 58, 59, 63, 66, 68, 76, 110, 3786, 9243]
surviving nonsolo ucounts = 2[3786, 9243]
ids = [302, 1394]

====================================================================================

REJECT CONTIGS

TIG 1[bases=317]
0-66 ==> 20-86 on segment before TRBJ2-2P exon 1 [len=86] (mis=0)
66-112 ==> 0-46 on |545|TRBJ2-2P|J-REGION| [len=46] (mis=0)
112-216 ==> 0-104 on segment before TRBJ2-3 exon 1 [len=104] (mis=0)
265-317 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
umis assigned: [1394]
of which 1 are surviving nonsolos
reads assigned: 9117
start codons at 23, 126
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.415 = GTCACGGTCGCGATCG-1

using 21 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.416 = GTCACGGTCGGCATCG-1

using 74 reads

====================================================================================

graph has 60 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[4, 10, 12, 46]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.424 = GTCACGGTCTAACTCT-1

using 60 reads

====================================================================================

graph has 84 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 9[2^2, 3, 4, 5, 7, 8, 13, 14]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.425 = GTCACGGTCTAACTTC-1

using 59 reads

====================================================================================

graph has 48 edges initially, 4 edges after simplification

total ucounts = 10
nonsolo ucounts = 6[2, 7^2, 11, 12, 16]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.431 = GTCACGGTCTGCCAGG-1

using 26 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.432 = GTCACGGTCTGGGCCA-1

using 53 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[23, 30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.439 = GTCACGGTCTTCGGTC-1

using 93 reads

====================================================================================

graph has 78 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[92]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.443 = GTCACGGTCTTGCCGT-1

using 88 reads

====================================================================================

graph has 118 edges initially, 2 edges after simplification

total ucounts = 13
nonsolo ucounts = 10[2^2, 3, 4, 5, 6^2, 8, 11, 38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.448 = GTCATTTAGAAGGGTA-1

using 57 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[56]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.449 = GTCATTTAGAATAGGG-1

using 123 reads

====================================================================================

graph has 58 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[123]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.450 = GTCATTTAGAATGTGT-1

using 28 reads

====================================================================================

graph has 52 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.451 = GTCATTTAGAATTCCC-1

using 46 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[46]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.455 = GTCATTTAGACTGTAA-1

using 23 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.456 = GTCATTTAGAGGGATA-1

using 28 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.459 = GTCATTTAGCAACGGT-1

using 1379 reads

====================================================================================

graph has 410 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[9, 1368]
surviving nonsolo ucounts = 1[1368]
ids = [0]

====================================================================================

UMI info for barcode GTCATTTAGCAACGGT-1 contig 1 = AGAGAAGTCT...
umi CGTTAAGCAA = 1387 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=516]
0-80 ==> 17-97 on |492|TRAV27|5'UTR| [len=97] (mis=0)
80-408 ==> 0-328 on |493|TRAV27|L-REGION+V-REGION| [len=328] (mis=2)
408-474 ==> 0-66 on |413|TRAJ28|J-REGION| [len=66] (mis=3)
474-516 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 1 umis using 66 reads
cdr3 = CAGEDSGAGSYQLTF at 398, score = 10 + 10
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 1357
start codons at 53, 62, 80, 124, 232, 333
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.461 = GTCATTTAGCCCAGCT-1

using 63 reads

====================================================================================

graph has 62 edges initially, 4 edges after simplification

total ucounts = 3
nonsolo ucounts = 3[4, 14, 45]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.466 = GTCATTTAGGCGACAT-1

using 50 reads

====================================================================================

graph has 52 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 5[4^2, 6, 14, 21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.467 = GTCATTTAGGGAGTAA-1

using 219 reads

====================================================================================

graph has 114 edges initially, 6 edges after simplification

total ucounts = 10
nonsolo ucounts = 8[4^3, 5, 14, 45, 52, 89]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.468 = GTCATTTAGGGATCTG-1

using 554 reads

====================================================================================

graph has 308 edges initially, 18 edges after simplification

total ucounts = 29
nonsolo ucounts = 26[2^3, 3, 4, 6, 7^2, 8^3, 9, 10, 13^2, 15, 16^2, 17, 29, 43, 50, 56, 61, 66, 80]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.471 = GTCATTTAGGGTTCCC-1

using 367 reads

====================================================================================

graph has 237 edges initially, 10 edges after simplification

total ucounts = 19
nonsolo ucounts = 15[2^2, 4, 6^2, 12, 15, 23, 28, 31, 33, 42, 43, 54, 62]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.473 = GTCATTTAGGTGCTTT-1

using 86 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[86]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.474 = GTCATTTAGTATGACA-1

using 113 reads

====================================================================================

graph has 92 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 5[5, 9, 12, 18, 66]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.477 = GTCATTTAGTCTCAAC-1

using 332 reads

====================================================================================

graph has 232 edges initially, 10 edges after simplification

total ucounts = 20
nonsolo ucounts = 17[2^4, 3, 5, 6^2, 9, 10, 16, 19, 32, 37, 40, 56, 82]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.478 = GTCATTTAGTGCGATG-1

using 39 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[3, 15, 19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.481 = GTCATTTAGTGTCTCA-1

using 14362 reads

====================================================================================

graph has 8587 edges initially, 222 edges after simplification

total ucounts = 982
nonsolo ucounts = 752[2^124, 3^87, 4^60, 5^48, 6^43, 7^47, 8^27, 9^43, 10^28, 11^28, 12^22, 13^28, 14^14, 15^22, 16^8, 17^13, 18^10, 19^13, 20^10, 21^6, 22^7, 23^5, 24^3, 25^5, 26^5, 27^3, 28^4, 29^3, 30, 31^3, 32^2, 33, 34^2, 35^4, 38^2, 39, 42^3, 43, 47, 50, 51, 53^2, 54^2, 55, 56, 59, 64, 77, 85, 163, 816, 5784]
surviving nonsolo ucounts = 3[163, 816, 5784]
ids = [473, 588, 850]

====================================================================================

REJECT CONTIGS

TIG 1[bases=374]
0-261 ==> 5739-6000 on segment before TRBD1 exon 1 [len=6000] (mis=0)
282-322 ==> 10-50 on |543|TRBJ2-1|J-REGION| [len=50] (mis=0)
322-374 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
umis assigned: [473, 588, 850]
of which 3 are surviving nonsolos
reads assigned: 6687
start codons at 138, 148, 222
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.484 = GTCATTTCAAAGGTGC-1

using 27 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.487 = GTCATTTCAAGAGGCT-1

using 55 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[55]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.488 = GTCATTTCAAGCGATG-1

using 18 reads

====================================================================================

graph has 6 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.491 = GTCATTTCAAGTCTGT-1

using 51 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[51]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.493 = GTCATTTCACAGGAGT-1

using 28 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.495 = GTCATTTCACCGTTGG-1

using 116 reads

====================================================================================

graph has 76 edges initially, 4 edges after simplification

total ucounts = 11
nonsolo ucounts = 6[4, 5, 7, 13, 28, 54]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.498 = GTCATTTCAGATCTGT-1

using 39 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[2^2, 34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.499 = GTCATTTCAGCCTATA-1

using 63 reads

====================================================================================

graph has 84 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[2, 3, 56]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.500 = GTCATTTCAGCGATCC-1

using 34 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.504 = GTCATTTCAGGGTACA-1

using 71 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[2, 5, 63]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.509 = GTCATTTCATAACCTG-1

using 31 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.510 = GTCATTTCATACGCTA-1

using 103 reads

====================================================================================

graph has 66 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[2, 99]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.512 = GTCATTTCATCGGAAG-1

using 43 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[2^2, 3, 34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.515 = GTCATTTCATGAACCT-1

using 62 reads

====================================================================================

graph has 64 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 4[7, 9, 13, 32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.516 = GTCATTTCATGATCCA-1

using 39 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[39]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.518 = GTCATTTCATGTTCCC-1

using 52 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 4[4, 5, 6, 36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.519 = GTCATTTCATGTTGAC-1

using 38 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.522 = GTCATTTCATTTGCTT-1

using 23 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.525 = GTCATTTGTAAGCACG-1

using 93 reads

====================================================================================

graph has 66 edges initially, 2 edges after simplification

total ucounts = 14
nonsolo ucounts = 7[3^2, 5, 8, 14, 17, 36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.526 = GTCATTTGTAATCACC-1

using 58 reads

====================================================================================

graph has 74 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 7[3^2, 4, 9, 11^2, 15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.527 = GTCATTTGTAATTGGA-1

using 31 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.529 = GTCATTTGTACTTAGC-1

using 138 reads

====================================================================================

graph has 164 edges initially, 2 edges after simplification

total ucounts = 12
nonsolo ucounts = 8[3^2, 7, 8^2, 14, 19, 72]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.530 = GTCATTTGTAGCGCTC-1

using 56 reads

====================================================================================

graph has 64 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[13, 14, 28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.531 = GTCATTTGTAGCGTGA-1

using 101 reads

====================================================================================

graph has 110 edges initially, 4 edges after simplification

total ucounts = 15
nonsolo ucounts = 10[2^2, 3, 4, 8, 11^2, 12, 17, 26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.535 = GTCATTTGTCACCTAA-1

using 44 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[44]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.536 = GTCATTTGTCACTTCC-1

using 57 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[57]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.541 = GTCATTTGTCCGAAGA-1

using 11373 reads

====================================================================================

graph has 7262 edges initially, 236 edges after simplification

total ucounts = 803
nonsolo ucounts = 615[2^82, 3^57, 4^60, 5^35, 6^30, 7^37, 8^23, 9^22, 10^17, 11^18, 12^13, 13^15, 14^11, 15^14, 16^13, 17^9, 18^12, 19^17, 20^14, 21^5, 22^9, 23^6, 24^8, 25^5, 26^8, 27^7, 28^2, 29^9, 30^4, 31^3, 32^2, 33^3, 34^3, 35^3, 36^4, 38, 40, 41^3, 42, 43^3, 44, 45, 46, 47^4, 48, 51, 52, 55, 56, 59, 61, 62, 67, 72, 73, 74, 75, 84, 93, 100, 119, 122, 3133]
surviving nonsolo ucounts = 1[3133]
ids = [242]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.542 = GTCATTTGTCCGAATT-1

using 37 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.546 = GTCATTTGTCTAGTCA-1

using 50 reads

====================================================================================

graph has 56 edges initially, 4 edges after simplification

total ucounts = 9
nonsolo ucounts = 7[2^2, 4^2, 9^2, 18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.549 = GTCATTTGTCTTCAAG-1

using 51 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[51]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.550 = GTCATTTGTCTTTCAT-1

using 39 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[39]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.553 = GTCATTTGTGCACTTA-1

using 68 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[68]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.554 = GTCATTTGTGGCTCCA-1

using 17631 reads

====================================================================================

graph has 9095 edges initially, 236 edges after simplification

total ucounts = 1017
nonsolo ucounts = 783[2^117, 3^84, 4^74, 5^52, 6^38, 7^49, 8^33, 9^40, 10^21, 11^26, 12^24, 13^23, 14^21, 15^18, 16^15, 17^9, 18^10, 19^8, 20^12, 21^6, 22^4, 23^6, 24^6, 25^6, 26^2, 27^4, 28, 29^3, 30^3, 31^4, 32^6, 33^5, 34^2, 35^4, 36^3, 37^3, 38^2, 39^2, 40, 42^3, 43, 44^3, 48, 50^2, 51^2, 54, 55^3, 56, 57, 58^2, 60, 62, 64, 68, 70, 73^2, 75, 79, 89, 93, 135, 138, 140, 1569, 6648]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.555 = GTCATTTGTGTCGCTG-1

using 119 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[119]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.556 = GTCATTTGTGTTTGTG-1

using 63 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[63]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.559 = GTCATTTGTTCAGACT-1

using 63 reads

====================================================================================

graph has 88 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 5[5, 7^2, 8, 35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.561 = GTCATTTGTTCCAACA-1

using 53 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[52]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.562 = GTCATTTGTTCGAATC-1

using 48 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[48]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.565 = GTCATTTGTTCTGGTA-1

using 68 reads

====================================================================================

graph has 56 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[68]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.566 = GTCATTTGTTCTGTTT-1

using 63 reads

====================================================================================

graph has 52 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[13, 50]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.567 = GTCATTTGTTGTACAC-1

using 132 reads

====================================================================================

graph has 120 edges initially, 6 edges after simplification

total ucounts = 7
nonsolo ucounts = 6[3, 5, 21, 24, 33, 45]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.570 = GTCATTTTCAACCAAC-1

using 75 reads

====================================================================================

graph has 112 edges initially, 2 edges after simplification

total ucounts = 17
nonsolo ucounts = 13[2^3, 4^5, 5, 6, 9, 11, 14]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.572 = GTCATTTTCACATAGC-1

using 100 reads

====================================================================================

graph has 90 edges initially, 2 edges after simplification

total ucounts = 15
nonsolo ucounts = 11[2, 3, 5, 6, 9^2, 11^2, 12, 13, 15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.573 = GTCATTTTCACATGCA-1

using 45 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[45]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.575 = GTCATTTTCACCATAG-1

using 72 reads

====================================================================================

graph has 80 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 7[2, 3, 5, 9, 10, 16, 25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.577 = GTCATTTTCACTCCTG-1

using 45 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[45]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.578 = GTCATTTTCACTTACT-1

using 74 reads

====================================================================================

graph has 106 edges initially, 2 edges after simplification

total ucounts = 12
nonsolo ucounts = 8[2, 3^2, 5, 6, 7, 11, 33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.579 = GTCATTTTCAGAGGTG-1

using 323 reads

====================================================================================

graph has 220 edges initially, 14 edges after simplification

total ucounts = 16
nonsolo ucounts = 13[2^2, 4^2, 5, 6^2, 12, 18, 52, 62, 72, 75]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.582 = GTCATTTTCATGCAAC-1

using 921 reads

====================================================================================

graph has 292 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 4[2^2, 3, 909]
surviving nonsolo ucounts = 1[909]
ids = [8]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.583 = GTCATTTTCATGCATG-1

using 58 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[3, 55]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.584 = GTCATTTTCATGCTCC-1

using 47 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[3, 44]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.586 = GTCATTTTCATTGCCC-1

using 38 reads

====================================================================================

graph has 6 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.587 = GTCATTTTCATTTGGG-1

using 103 reads

====================================================================================

graph has 52 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[103]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.588 = GTCATTTTCCAAGTAC-1

using 57 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 6[2, 7, 9, 11, 13, 14]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.590 = GTCATTTTCCATTCTA-1

using 24 reads

====================================================================================

graph has 48 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.591 = GTCATTTTCCCAAGAT-1

using 52 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[51]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.596 = GTCATTTTCCGCATCT-1

using 94 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[94]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.600 = GTCATTTTCGCAAGCC-1

using 24 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.601 = GTCATTTTCGCATGGC-1

using 22 reads

====================================================================================

graph has 6 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.603 = GTCATTTTCGCGGATC-1

using 38 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.605 = GTCATTTTCGGAATCT-1

using 53 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[53]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.606 = GTCATTTTCGGCTACG-1

using 53 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[53]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.607 = GTCATTTTCGGTGTTA-1

using 28 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 1[26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.609 = GTCATTTTCGTCTGCT-1

using 23 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.613 = GTCATTTTCTAGAGTC-1

using 37 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.614 = GTCATTTTCTCCAGGG-1

using 251 reads

====================================================================================

graph has 140 edges initially, 8 edges after simplification

total ucounts = 15
nonsolo ucounts = 11[2, 3, 4, 8, 10, 12, 16, 22, 41^2, 88]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.617 = GTCATTTTCTCTGCTG-1

using 80 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[80]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.618 = GTCATTTTCTGAAAGA-1

using 100 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[100]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.619 = GTCATTTTCTGAGTGT-1

using 17 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[17]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.620 = GTCATTTTCTGCAAGT-1

using 12122 reads

====================================================================================

graph has 3433 edges initially, 36 edges after simplification

total ucounts = 169
nonsolo ucounts = 119[2^18, 3^17, 4^8, 5^5, 6^7, 7^3, 8^6, 9^4, 10^6, 11^2, 12^4, 13, 14^3, 15^3, 16, 17^3, 18^2, 19^2, 20, 21, 22^3, 23, 24^2, 27, 29^3, 31, 32, 35, 37, 43, 44, 51, 71, 76, 204, 5112, 5382]
surviving nonsolo ucounts = 2[5112, 5382]
ids = [155, 65]

====================================================================================

REJECT CONTIGS

TIG 1[bases=377]
5-272 ==> 80-347 on |592|TRBV2|L-REGION+V-REGION| [len=347] (mis=4)
276-325 ==> 0-49 on |546|TRBJ2-3|J-REGION| [len=49] (mis=5)
325-377 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
cdr3 = CASRGGVARDTQYF at 255, score = 6 + 8
umis assigned: [65, 155]
of which 2 are surviving nonsolos
reads assigned: 9543
start codons at 24, 136, 166, 193, 246
confident = false
not full
VJ delta = 18
not full
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.621 = GTCATTTTCTGCTGTC-1

using 52 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[52]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.622 = GTCATTTTCTGTCCGT-1

using 31 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.623 = GTCATTTTCTTCAACT-1

using 85 reads

====================================================================================

graph has 122 edges initially, 2 edges after simplification

total ucounts = 15
nonsolo ucounts = 8[2^2, 3, 4^2, 10, 11, 42]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.625 = GTCATTTTCTTCGGTC-1

using 43 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[43]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.628 = GTCCTCAAGAATGTGT-1

using 164 reads

====================================================================================

graph has 94 edges initially, 4 edges after simplification

total ucounts = 5
nonsolo ucounts = 4[6, 12, 49, 96]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.629 = GTCCTCAAGACCGGAT-1

using 53 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 3[13, 15, 25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.633 = GTCCTCAAGATAGTCA-1

using 197 reads

====================================================================================

graph has 84 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[197]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.635 = GTCCTCAAGATGAGAG-1

using 92 reads

====================================================================================

graph has 44 edges initially, 4 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[29, 62]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.638 = GTCCTCAAGCACAGGT-1

using 15 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.645 = GTCCTCAAGCGCTTAT-1

using 63 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[63]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.647 = GTCCTCAAGCTAACAA-1

using 30 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.648 = GTCCTCAAGCTCCCAG-1

using 89 reads

====================================================================================

graph has 46 edges initially, 4 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[23, 66]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.652 = GTCCTCAAGGATTCGG-1

using 721 reads

====================================================================================

graph has 500 edges initially, 10 edges after simplification

total ucounts = 27
nonsolo ucounts = 25[2^2, 3, 4, 5, 6, 7, 8, 9, 12, 18, 20^2, 21, 26, 27, 33, 34, 43, 52, 61, 68, 69, 82, 87]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.658 = GTCCTCAAGGGCTCTC-1

using 39 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.660 = GTCCTCAAGGGTTTCT-1

using 18 reads

====================================================================================

graph has 6 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.661 = GTCCTCAAGGTAGCTG-1

using 7748 reads

====================================================================================

graph has 6526 edges initially, 220 edges after simplification

total ucounts = 833
nonsolo ucounts = 599[2^95, 3^53, 4^45, 5^41, 6^34, 7^23, 8^30, 9^21, 10^22, 11^21, 12^17, 13^20, 14^15, 15^9, 16^11, 17^18, 18^11, 19^9, 20^6, 21^4, 22^5, 23^5, 24^7, 25^4, 26^5, 27^4, 28^4, 29^5, 30^8, 33^6, 34, 35, 36, 37, 38^6, 39, 41, 42^4, 45, 46, 47, 49^2, 53, 54^3, 55^2, 56^2, 60, 61, 63, 66^3, 67, 69, 92, 99, 121, 124]
surviving nonsolo ucounts = 3[2, 99, 121]
ids = [32, 790, 108]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.665 = GTCCTCAAGGTGCTTT-1

using 64978 reads

====================================================================================

graph has 27044 edges initially, 588 edges after simplification

total ucounts = 2166
nonsolo ucounts = 1639[2^224, 3^179, 4^126, 5^100, 6^100, 7^77, 8^76, 9^72, 10^55, 11^47, 12^56, 13^38, 14^46, 15^40, 16^31, 17^30, 18^27, 19^20, 20^15, 21^14, 22^23, 23^18, 24^15, 25^13, 26^10, 27^5, 28^11, 29^11, 30^8, 31^10, 32^5, 33^10, 34^4, 35^7, 36^8, 37^3, 38^8, 39^6, 40^4, 41^4, 42^3, 43^5, 44^6, 45, 46, 47^4, 48, 50^2, 51, 52, 53, 55, 56^2, 57^3, 58, 59^2, 60, 61^2, 65^2, 66^3, 68^2, 70, 73, 75^2, 77, 80^2, 93, 100, 102, 106, 110, 127, 183, 409, 557, 824, 1038, 1079, 1231, 1246, 1249, 1280, 1379, 1380, 1406, 1583, 1591, 1672^2, 1728, 1853, 1993, 3147, 3276, 3510, 3514, 6499]
surviving nonsolo ucounts = 25[127, 409, 557, 824, 1038, 1079, 1231, 1246, 1249, 1280, 1379, 1380, 1406, 1583, 1591, 1672^2, 1728, 1853, 1993, 3147, 3276, 3510, 3514, 6499]
ids = [1202, 1670, 1607, 1867, 297, 1485, 1288, 749, 834, 1712, ...]

====================================================================================

UMI info for barcode GTCCTCAAGGTGCTTT-1 contig 1 = ATGCTCACAG...
umi AGGGCCCGCG = 1042 reads: +406 validated
umi ATAATGTGTA = 1600 reads: +406 validated
umi ATCATCTCTA = 1408 reads: +406 validated
umi CAACTCATTA = 2011 reads: +406 validated
umi CCAGGACGTT = 1757 reads: +150 -1XX +255 invalidated
umi CCTCTGGTCT = 1252 reads: +406 validated
umi CGCTTCTATA = 1242 reads: +406 validated
umi CGGTATCTGG = 1866 reads: +406 validated
umi CTCGCTCTAA = 1582 reads: +406 validated
umi CTGCGTTGCG = 1391 reads: +406 validated
umi GCGTGGCGCG = 1388 reads: +406 validated
umi GCTTATTGCG = 1722 reads: +406 validated
umi GGAAACGTAC = 1231 reads: +406 validated
umi GTTCGCGCAA = 1672 reads: +406 validated
umi TAACACCCAA = 1102 reads: +406 validated
umi TATATTTGGT = 565 reads: +406 validated
umi TCCCCCCACT = 1333 reads: +123 -5XX +278 invalidated
umi TGCCTTGCTC = 829 reads: +406 validated

GOOD CONTIGS

TIG 1[bases=510]
0-52 ==> 10-62 on |565|TRBV12-3|5'UTR| [len=62] (mis=0)
52-392 ==> 0-340 on |566|TRBV12-3|L-REGION+V-REGION| [len=347] (mis=0)
408-458 ==> 0-50 on |540|TRBJ1-5|J-REGION| [len=50] (mis=2)
458-510 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 18 umis using 1563 reads
cdr3 = CASNSNGGPNNQPQHF at 382, score = 8 + 8
umis assigned: [297, 332, 366, 491, 634, 749, 834, 853, 959, 1005] and 8 others
of which 18 are surviving nonsolos
reads assigned: 24429
start codons at 0, 52, 110, 137, 151, 174, 223, 226, 275, 286, 313, 320, 398, 434
confident = true

REJECT CONTIGS

TIG 1[bases=335]
1-230 ==> 211-440 on segment before TRBJ1-6 exon 1 [len=440] (mis=0)
230-283 ==> 0-53 on |541|TRBJ1-6|J-REGION| [len=53] (mis=0)
283-335 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
umis assigned: [1670, 1884, 2026]
of which 3 are surviving nonsolos
reads assigned: 8957
start codons at 24, 30, 69, 81, 125
confident = false
did not find CDR3

TIG 2[bases=441]
5-34 ==> 4241-4270 on segment before TRAV36DV7 exon 1 [len=4270] (mis=0)
50-390 ==> 0-340 on |503|TRAV36/DV7|L-REGION+V-REGION| [len=340] (mis=4)
399-441 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
umis assigned: [65, 1202]
of which 2 are surviving nonsolos
reads assigned: 3337
start codons at 50, 53, 186, 214, 254
confident = false
did not find CDR3

TIG 3[bases=441]
0-43 ==> 0-43 on |529|TRAV9-2|5'UTR| [len=56] (mis=4)
41-334 ==> 45-338 on |530|TRAV9-2|L-REGION+V-REGION| [len=338] (mis=3)
333-399 ==> 0-66 on |440|TRAJ53|J-REGION| [len=66] (mis=2)
399-441 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
cdr3 = CALRGNSGGSNYKLTF at 320, score = 9 + 9
umis assigned: [294, 372, 1003]
of which 2 are surviving nonsolos
reads assigned: 6419
start codons at 15, 71, 159, 213
confident = false
VJ delta = 29
not full
not full
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.667 = GTCCTCAAGTATCGAA-1

using 13288 reads

====================================================================================

graph has 6942 edges initially, 178 edges after simplification

total ucounts = 627
nonsolo ucounts = 495[2^59, 3^45, 4^41, 5^30, 6^27, 7^26, 8^24, 9^19, 10^20, 11^26, 12^9, 13^18, 14^11, 15^12, 16^15, 17^8, 18^12, 19^4, 20^10, 21^8, 22^4, 23^8, 24^4, 25^2, 26^2, 27, 28^3, 29^3, 30^4, 31, 32^3, 33, 34^4, 35^3, 36^2, 37^3, 38^2, 39^3, 40, 41, 42^2, 43, 44, 46^2, 48, 49, 50, 51, 56, 63, 88, 96, 1480, 5794]
surviving nonsolo ucounts = 3[96, 1480, 5794]
ids = [258, 35, 197]

====================================================================================

UMI info for barcode GTCCTCAAGTATCGAA-1 contig 1 = ACAGAAGACC...
umi AGGTATCCAT = 1508 reads: +391 validated
umi CGTAATCGTT = 4952 reads: -354 +4 -2XX +2 -1XX +2 -1XX +5 -1XX +2 -1XX +5 -1XX +2 -1XX +5 -1XX +1 invalidated
umi CGTTGTCCAG = 2 reads: -387 +1 -2XX +1 invalidated

GOOD CONTIGS

TIG 1[bases=475]
0-32 ==> 34-66 on |657|TRBV6-5|5'UTR| [len=66] (mis=0)
32-376 ==> 0-344 on |658|TRBV6-5|L-REGION+V-REGION| [len=344] (mis=2)
382-423 ==> 9-50 on |543|TRBJ2-1|J-REGION| [len=50] (mis=0)
423-475 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 1 umis using 96 reads
cdr3 = CASSYDGLEQFF at 359, score = 9 + 8
umis assigned: [35, 197, 205]
of which 2 are surviving nonsolos
reads assigned: 6351
start codons at 18, 32, 90, 143, 167, 174, 182, 209, 273, 282
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.669 = GTCCTCAAGTGCGTGA-1

using 340 reads

====================================================================================

graph has 246 edges initially, 10 edges after simplification

total ucounts = 13
nonsolo ucounts = 11[4, 6, 8^3, 12, 18, 53, 58, 62, 101]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.670 = GTCCTCAAGTGGAGAA-1

using 417 reads

====================================================================================

graph has 252 edges initially, 6 edges after simplification

total ucounts = 15
nonsolo ucounts = 12[2, 4^2, 7, 9, 14, 36, 41, 46, 60, 83, 108]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.673 = GTCCTCAAGTGTTAGA-1

using 16 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[16]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.677 = GTCCTCACAAACTGCT-1

using 76 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[76]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.678 = GTCCTCACAAATTGCC-1

using 69 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[69]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.686 = GTCCTCACAAGTTGTC-1

using 24 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 1[21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.688 = GTCCTCACAATCAGAA-1

using 24 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.691 = GTCCTCACACAACGCC-1

using 71 reads

====================================================================================

graph has 36 edges initially, 4 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[17, 53]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.692 = GTCCTCACACACCGCA-1

using 371 reads

====================================================================================

graph has 186 edges initially, 12 edges after simplification

total ucounts = 19
nonsolo ucounts = 16[2^5, 3, 5, 6, 7, 8, 22, 27, 34, 70, 88^2]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.694 = GTCCTCACACATCCAA-1

using 114 reads

====================================================================================

graph has 104 edges initially, 10 edges after simplification

total ucounts = 10
nonsolo ucounts = 8[2, 3^2, 4, 16, 18, 30, 36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.701 = GTCCTCACACGAAGCA-1

using 6671 reads

====================================================================================

graph has 5871 edges initially, 214 edges after simplification

total ucounts = 773
nonsolo ucounts = 577[2^92, 3^63, 4^53, 5^46, 6^41, 7^21, 8^24, 9^18, 10^17, 11^15, 12^17, 13^15, 14^10, 15^13, 16^10, 17^11, 18^8, 19^3, 20^6, 21^7, 22^8, 23^3, 24^6, 25^2, 26^3, 27^6, 28^3, 29^4, 30^3, 31^6, 32^3, 33^5, 34^5, 35^4, 36, 37, 38^5, 41, 42^2, 44, 45, 46, 47^2, 48, 49, 53, 54, 58, 61, 62, 63, 69, 76, 93]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.707 = GTCCTCACAGACGCAA-1

using 218 reads

====================================================================================

graph has 158 edges initially, 10 edges after simplification

total ucounts = 16
nonsolo ucounts = 14[2, 3, 4, 6^2, 8, 9, 10, 13, 14, 18, 25, 29, 69]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.710 = GTCCTCACAGATCTGT-1

using 708 reads

====================================================================================

graph has 626 edges initially, 28 edges after simplification

total ucounts = 43
nonsolo ucounts = 35[2^4, 3, 4, 5^4, 6, 7, 8^2, 10, 11, 14, 17, 18, 19, 20, 21^2, 23^3, 25^3, 35, 41, 49, 66, 70, 80]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.714 = GTCCTCACAGGATTGG-1

using 111 reads

====================================================================================

graph has 62 edges initially, 4 edges after simplification

total ucounts = 9
nonsolo ucounts = 4[2^2, 22, 80]
surviving nonsolo ucounts = 1[80]
ids = [4]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.719 = GTCCTCACAGTGGAGT-1

using 109 reads

====================================================================================

graph has 70 edges initially, 4 edges after simplification

total ucounts = 6
nonsolo ucounts = 5[2, 3, 11, 34, 58]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.724 = GTCCTCACATCACGTA-1

using 81 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[81]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.726 = GTCCTCACATCCCACT-1

using 15 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.727 = GTCCTCACATCCGGGT-1

using 38 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.728 = GTCCTCACATCGGGTC-1

using 21 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.729 = GTCCTCACATCTCCCA-1

using 66 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 5[2, 3, 6, 8, 45]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.730 = GTCCTCACATGCCTTC-1

using 50 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[50]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.731 = GTCCTCACATGCGCAC-1

using 116 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 2[2, 111]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.732 = GTCCTCACATGGTCAT-1

using 59 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 5[2^2, 4, 5, 45]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.733 = GTCCTCACATGTCCTC-1

using 48 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[9, 38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.735 = GTCCTCACATTATCTC-1

using 75 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 5[2, 5, 7, 10, 50]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.736 = GTCCTCACATTCACTT-1

using 135 reads

====================================================================================

graph has 96 edges initially, 4 edges after simplification

total ucounts = 14
nonsolo ucounts = 9[2, 4, 5, 6, 9^2, 11^2, 73]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.738 = GTCCTCACATTCTCAT-1

using 52 reads

====================================================================================

graph has 54 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[24, 28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.739 = GTCCTCACATTGAGCT-1

using 249 reads

====================================================================================

graph has 182 edges initially, 14 edges after simplification

total ucounts = 16
nonsolo ucounts = 11[3, 4, 6, 12, 14, 18, 22, 25, 35, 41, 64]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.743 = GTCCTCAGTAAGAGAG-1

using 18 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.748 = GTCCTCAGTACCAGTT-1

using 108 reads

====================================================================================

graph has 64 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[108]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.752 = GTCCTCAGTAGAAGGA-1

using 227 reads

====================================================================================

graph has 212 edges initially, 6 edges after simplification

total ucounts = 22
nonsolo ucounts = 17[2^6, 3^2, 6, 7, 8, 11, 16, 18, 26, 47, 65]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.753 = GTCCTCAGTAGAGGAA-1

using 50 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 5[3, 4^2, 6, 32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.755 = GTCCTCAGTAGCGTAG-1

using 103 reads

====================================================================================

graph has 84 edges initially, 4 edges after simplification

total ucounts = 9
nonsolo ucounts = 7[2^2, 5^2, 12, 32, 43]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.758 = GTCCTCAGTAGTACCT-1

using 51 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[51]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.762 = GTCCTCAGTCAAACTC-1

using 668 reads

====================================================================================

graph has 558 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[667]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.764 = GTCCTCAGTCAACTGT-1

using 24 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.769 = GTCCTCAGTCCCTACT-1

using 3959 reads

====================================================================================

graph has 4316 edges initially, 133 edges after simplification

total ucounts = 524
nonsolo ucounts = 348[2^66, 3^52, 4^28, 5^25, 6^20, 7^19, 8^16, 9^12, 10^7, 11^8, 12^6, 13^9, 14^9, 15^5, 16^9, 17^2, 18^2, 19^3, 20^8, 21^2, 22^3, 23^2, 24, 25^3, 26^2, 27^4, 28, 29, 30, 34^2, 35, 36, 37, 38^2, 41, 42, 44^2, 45, 55, 57, 59, 63, 65, 67, 72^2, 73, 238]
surviving nonsolo ucounts = 2[3, 238]
ids = [48, 509]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.776 = GTCCTCAGTCGGCTCA-1

using 22 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.783 = GTCCTCAGTCTTTCAT-1

using 110 reads

====================================================================================

graph has 101 edges initially, 6 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[14, 19, 30, 45]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.784 = GTCCTCAGTGACTACT-1

using 50 reads

====================================================================================

graph has 64 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 3[2^2, 46]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.787 = GTCCTCAGTGCCTGTG-1

using 55 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[54]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.791 = GTCCTCAGTGTCAATC-1

using 70 reads

====================================================================================

graph has 110 edges initially, 2 edges after simplification

total ucounts = 13
nonsolo ucounts = 8[2^4, 3^2, 6, 45]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.792 = GTCCTCAGTGTCCTCT-1

using 40 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[40]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.795 = GTCCTCAGTGTTTGGT-1

using 24 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.796 = GTCCTCAGTTACGTCA-1

using 13 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[13]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.800 = GTCCTCAGTTCAGACT-1

using 31 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[7, 24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.806 = GTCCTCAGTTCTCATT-1

using 65 reads

====================================================================================

graph has 70 edges initially, 2 edges after simplification

total ucounts = 12
nonsolo ucounts = 10[2^3, 3^2, 4, 5, 6^2, 30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.807 = GTCCTCAGTTCTGTTT-1

using 97 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[97]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.823 = GTCCTCATCACTCCTG-1

using 60 reads

====================================================================================

graph has 62 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 5[2, 5, 12, 14, 23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.824 = GTCCTCATCAGATAAG-1

using 155 reads

====================================================================================

graph has 96 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 6[2^2, 5, 7, 8, 129]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.828 = GTCCTCATCATCGATG-1

using 33 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.830 = GTCCTCATCATCTGTT-1

using 101 reads

====================================================================================

graph has 86 edges initially, 4 edges after simplification

total ucounts = 9
nonsolo ucounts = 7[2^2, 8, 13, 17, 22, 35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.831 = GTCCTCATCATGCTCC-1

using 81 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[80]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.832 = GTCCTCATCATGGTCA-1

using 45 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[6, 39]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.834 = GTCCTCATCCAAAGTC-1

using 80 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[80]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.838 = GTCCTCATCCCTCAGT-1

using 136 reads

====================================================================================

graph has 116 edges initially, 2 edges after simplification

total ucounts = 18
nonsolo ucounts = 13[2^2, 3^2, 4, 5, 6^2, 8, 9, 17, 24, 42]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.840 = GTCCTCATCCGGCACA-1

using 94 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[94]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.842 = GTCCTCATCCGTCATC-1

using 77 reads

====================================================================================

graph has 68 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 4[2, 3, 4, 63]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.844 = GTCCTCATCCTCAATT-1

using 295 reads

====================================================================================

graph has 316 edges initially, 6 edges after simplification

total ucounts = 50
nonsolo ucounts = 34[2^6, 3^11, 4^2, 5^2, 6^2, 7, 8^3, 9, 10, 13, 28, 30, 36, 47]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.846 = GTCCTCATCCTCGCAT-1

using 69 reads

====================================================================================

graph has 72 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 3[4, 5, 55]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.855 = GTCCTCATCGTATCAG-1

using 46 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[46]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.856 = GTCCTCATCGTCACGG-1

using 36 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.857 = GTCCTCATCGTCGTTC-1

using 10 reads

====================================================================================

graph has 6 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[10]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.858 = GTCCTCATCGTCTGCT-1

using 33 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.861 = GTCCTCATCTAACTTC-1

using 32 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.864 = GTCCTCATCTAGAGTC-1

using 529 reads

====================================================================================

graph has 162 edges initially, 4 edges after simplification

total ucounts = 6
nonsolo ucounts = 6[2, 3, 5, 13, 55, 451]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.866 = GTCCTCATCTCAAACG-1

using 162 reads

====================================================================================

graph has 186 edges initially, 4 edges after simplification

total ucounts = 19
nonsolo ucounts = 11[3, 4^2, 5^2, 9, 10, 15, 22, 38, 39]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.867 = GTCCTCATCTCACATT-1

using 111 reads

====================================================================================

graph has 58 edges initially, 4 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[28, 83]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.868 = GTCCTCATCTCTAAGG-1

using 36 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.870 = GTCCTCATCTGCTGCT-1

using 83 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[83]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.872 = GTCCTCATCTGGTATG-1

using 37 reads

====================================================================================

graph has 66 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 7[2^3, 3, 6, 7, 11]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.873 = GTCCTCATCTGGTTCC-1

using 51 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[2, 47]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.874 = GTCCTCATCTGTCAAG-1

using 68 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[68]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.875 = GTCCTCATCTTCAACT-1

using 18 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[3^2, 11]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.877 = GTCCTCATCTTCCTTC-1

using 216 reads

====================================================================================

graph has 124 edges initially, 10 edges after simplification

total ucounts = 3
nonsolo ucounts = 3[18, 23, 175]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.878 = GTCCTCATCTTCGAGA-1

using 38 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.879 = GTCCTCATCTTGCAAG-1

using 31 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[2, 3, 5, 19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.885 = GTCGGGTAGCTGAACG-1

using 35 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.890 = GTCGGGTCAAAGCGGT-1

using 24 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.898 = GTCGGGTCACCAGTTA-1

using 41 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[41]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.909 = GTCGGGTCAGACGTAG-1

using 48 reads

====================================================================================

graph has 64 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 4[2^2, 3, 37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.910 = GTCGGGTCAGATCCAT-1

using 25 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.912 = GTCGGGTCAGATCTGT-1

using 102 reads

====================================================================================

graph has 68 edges initially, 4 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[5, 15, 18, 62]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.925 = GTCGGGTCAGGTGGAT-1

using 690 reads

====================================================================================

graph has 280 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[3, 686]
surviving nonsolo ucounts = 1[686]
ids = [1]

====================================================================================

UMI info for barcode GTCGGGTCAGGTGGAT-1 contig 1 = CATGTTCACA...
umi GAGATTCCGA = 688 reads: +403 validated

GOOD CONTIGS

TIG 1[bases=508]
0-53 ==> 6-59 on |567|TRBV12-4|5'UTR| [len=59] (mis=0)
53-400 ==> 0-347 on |568|TRBV12-4|L-REGION+V-REGION| [len=347] (mis=2)
407-456 ==> 0-49 on |546|TRBJ2-3|J-REGION| [len=49] (mis=2)
456-508 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 1 umis using 52 reads
cdr3 = CASSLGLAGTDTQYF at 383, score = 8 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 677
start codons at 1, 53, 111, 152, 175, 224, 227, 276, 287, 314, 321
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.938 = GTCGGGTCATGGGAAC-1

using 56 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[56]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.943 = GTCGGGTGTAAGGATT-1

using 20 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.949 = GTCGGGTGTAGCCTAT-1

using 47 reads

====================================================================================

graph has 66 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 7[2^2, 3^4, 27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.953 = GTCGGGTGTATCAGTC-1

using 251 reads

====================================================================================

graph has 130 edges initially, 4 edges after simplification

total ucounts = 48
nonsolo ucounts = 29[2^11, 3^4, 5^2, 6, 7^2, 8^3, 9, 10, 16, 22, 30, 57]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.954 = GTCGGGTGTATGCTTG-1

using 80 reads

====================================================================================

graph has 68 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 7[2, 3^2, 9, 16, 19, 24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.957 = GTCGGGTGTCGAAAGC-1

using 115 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 4[2^2, 3, 101]
surviving nonsolo ucounts = 1[101]
ids = [3]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.958 = GTCGGGTGTCGACTGC-1

using 51 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[2, 49]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.961 = GTCGGGTGTCGGGTCT-1

using 31 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.963 = GTCGGGTGTCTCTCTG-1

using 132 reads

====================================================================================

graph has 112 edges initially, 6 edges after simplification

total ucounts = 11
nonsolo ucounts = 7[2, 4, 6, 11, 17, 41, 47]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.975 = GTCGGGTGTTCCGGCA-1

using 38 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.977 = GTCGGGTGTTGCTCCT-1

using 2507 reads

====================================================================================

graph has 1835 edges initially, 48 edges after simplification

total ucounts = 346
nonsolo ucounts = 207[2^68, 3^42, 4^25, 5^14, 6^10, 7^9, 8^5, 9^4, 10^2, 11^3, 12^2, 13, 14, 15^2, 16, 19, 21^3, 22, 28, 31, 33, 34, 38, 40, 41, 43, 46, 58, 60, 93, 938]
surviving nonsolo ucounts = 2[93, 938]
ids = [335, 234]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.980 = GTCGGGTGTTTAGGAA-1

using 19 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.981 = GTCGGGTGTTTGACTG-1

using 1451 reads

====================================================================================

graph has 260 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[3, 7, 1439]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.991 = GTCGGGTTCATCACCC-1

using 54 reads

====================================================================================

graph has 60 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[54]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.996 = GTCGGGTTCCGAATGT-1

using 18202 reads

====================================================================================

graph has 6988 edges initially, 104 edges after simplification

total ucounts = 703
nonsolo ucounts = 446[2^112, 3^90, 4^43, 5^37, 6^33, 7^21, 8^18, 9^8, 10^12, 11^10, 12^8, 13^5, 14^2, 15^5, 16^4, 17, 18^2, 19^2, 20, 21, 22^2, 23, 25, 27, 28, 35, 39, 42, 50, 69, 71, 72, 75, 198, 382, 495, 528, 538, 544, 636, 716, 721, 750, 853, 879, 967, 1042, 1078, 1506, 3410]
surviving nonsolo ucounts = 17[198, 382, 495, 528, 538, 544, 636, 716, 721, 750, 853, 879, 967, 1042, 1078, 1506, 3410]
ids = [444, 165, 655, 305, 252, 509, 159, 437, 39, 11, ...]

====================================================================================

UMI info for barcode GTCGGGTTCCGAATGT-1 contig 1 = ACAGTGATCC...
umi AACCCATCCA = 788 reads: +400 validated
umi ACAAGTGTTA = 708 reads: +400 validated
umi ACATGGAGCA = 884 reads: +400 validated
umi ATGTTTATTG = 639 reads: +400 validated
umi ATTCTTCTTT = 373 reads: +317 -1XX +18 -7X +2 -43 +1 -2XX +1 -1XX +2 -2XX +1 -1XX +1 invalidated
umi CCCTTAAAGG = 1000 reads: +317 -1XX +18 -7XX +2 -40XX +1 -2X +1 -2XX +1 -1XX +2 -2XX +1 -1XX +1 invalidated
umi CCCTTCAGTT = 851 reads: +400 validated
umi CCTATCTCGA = 548 reads: +400 validated
umi CGTGGGAATT = 532 reads: +400 validated
umi GCACTTTCCG = 1087 reads: +400 validated
umi GGCCGTCTTA = 714 reads: +400 validated
umi TACAGGAGTA = 528 reads: +400 validated
umi TGTTTCTACC = 3012 reads: -379 +5 -1XX +2 -1XX +1 -2XX +1 -1XX +2 -2XX +1 -1XX +1 invalidated
umi TTACGTGTGA = 492 reads: +400 validated

UMI info for barcode GTCGGGTTCCGAATGT-1 contig 2 = CATTATTTGG...
umi GCTCTACCCC = 1410 reads: -360 +5 -4XX +4 -2XX +1 -3XX +2 -3XX +2 -4XX +2 -2XX invalidated
umi GCTTCTAGTA = 970 reads: +394 validated
umi GGCTTTATCC = 197 reads: -23X +21 -1XX +349 invalidated

GOOD CONTIGS

TIG 1[bases=499]
0-47 ==> 0-47 on |674|TRBV7-2|5'UTR| [len=47] (mis=0)
47-394 ==> 0-347 on |675|TRBV7-2|L-REGION+V-REGION| [len=347] (mis=4)
399-447 ==> 0-48 on |537|TRBJ1-2|J-REGION| [len=48] (mis=0)
447-499 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 11 umis using 465 reads
cdr3 = CASSTTTANYGYTF at 377, score = 8 + 9
umis assigned: [11, 39, 47, 159, 165, 241, 243, 252, 305, 398] and 4 others
of which 14 are surviving nonsolos
reads assigned: 11969
start codons at 47, 156, 405
confident = true

TIG 2[bases=460]
0-24 ==> 32-56 on |529|TRAV9-2|5'UTR| [len=56] (mis=0)
24-362 ==> 0-338 on |530|TRAV9-2|L-REGION+V-REGION| [len=338] (mis=6)
356-418 ==> 0-62 on |442|TRAJ56|J-REGION| [len=62] (mis=4)
418-460 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 2 umis using 71 reads
cdr3 = CALATGANSKLTF at 348, score = 9 + 8
umis assigned: [419, 421, 444]
of which 3 are surviving nonsolos
reads assigned: 2555
start codons at 24, 99, 187, 241
confident = true
now this is a cell
paired!

GTTCAAGTGTCAGACTCAGCGGTGTACTTCTGTGCTCTGGCAACTGGAGCCAATAGTAAGCTGACATTTGGAAAAGGAATAACTCTGAGTGTTAGACCAG <==> ACACAGCAGGAGGACTCGGCCGTGTATCTCTGTGCCAGCAGCACCACGACTGCTAACTATGGCTACACCTTCGGTTCGGGGACCAGGTTAACCGTTGTAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1009 = GTCGGGTTCGTAGATC-1

using 5465 reads

====================================================================================

graph has 3627 edges initially, 100 edges after simplification

total ucounts = 802
nonsolo ucounts = 488[2^138, 3^81, 4^48, 5^31, 6^38, 7^17, 8^20, 9^7, 10^5, 11^18, 12^3, 13^8, 14^5, 15^5, 16^2, 17^5, 18^2, 20^4, 21^2, 22^3, 23^3, 24^3, 25, 26^2, 27^2, 28, 29^2, 30, 31^3, 32^2, 33, 34^2, 35^4, 36, 38^2, 40, 41^2, 43, 46, 47^2, 48^2, 51, 52, 56, 59, 65, 505, 753]
surviving nonsolo ucounts = 2[505, 753]
ids = [478, 608]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1020 = GTCGGGTTCTTGTATC-1

using 23779 reads

====================================================================================

graph has 7268 edges initially, 100 edges after simplification

total ucounts = 701
nonsolo ucounts = 453[2^95, 3^63, 4^63, 5^48, 6^26, 7^20, 8^26, 9^18, 10^11, 11^13, 12^14, 13^9, 14^10, 15^3, 16^2, 17^2, 18, 19^2, 20, 21, 22^2, 23, 25, 26, 28, 36, 39, 46^2, 49, 71, 128, 382, 518, 601, 625, 832, 1017, 1471, 2194, 2215, 2248, 4067, 4377]
surviving nonsolo ucounts = 12[382, 518, 601, 625, 832, 1017, 1471, 2194, 2215, 2248, 4067, 4377]
ids = [379, 682, 62, 475, 48, 634, 80, 409, 9, 81, ...]

====================================================================================

UMI info for barcode GTCGGGTTCTTGTATC-1 contig 1 = GGGAGGATTG...
umi AACAACGCGC = 2338 reads: +99 -2XX +1 -3XX +1 -1XX +2 -7XX +1 -1XX +1 -48XX +1 -12XX +232 invalidated
umi AATGTTAGAA = 825 reads: +412 validated
umi ACCAAAGCAC = 611 reads: +412 validated
umi ACGATTCTGT = 1484 reads: -48X +364 invalidated
umi ACGCGTTGCA = 2363 reads: +99 -2XX +1 -3XX +1 -1XX +2 -7XX +1 -1XX +1 -28XX +1 -2X +2 -15X +1 -12XX +232 invalidated
umi GATCCCCTCA = 389 reads: +412 validated
umi GCGGTAGGCG = 2202 reads: -52X +360 invalidated
umi TGGTTCGGAC = 1023 reads: +412 validated

GOOD CONTIGS

TIG 1[bases=593]
129-491 ==> 0-362 on |739|TRBV7-7|L-REGION+V-REGION| [len=362] (mis=3)
493-541 ==> 0-48 on |548|TRBJ2-5|J-REGION| [len=48] (mis=3)
541-593 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 8 umis using 849 reads
cdr3 = CASSLTIRETQYF at 474, score = 5 + 7
umis assigned: [9, 48, 62, 80, 81, 379, 409, 634]
of which 8 are surviving nonsolos
reads assigned: 10825
start codons at 22, 129, 146, 253, 286, 352, 465
confident = true

REJECT CONTIGS

TIG 1[bases=330]
8-208 ==> 5800-6000 on segment before TRBD1 exon 1 [len=6000] (mis=0)
225-278 ==> 0-53 on |549|TRBJ2-6|J-REGION| [len=53] (mis=1)
278-330 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
umis assigned: [194, 501, 682]
of which 3 are surviving nonsolos
reads assigned: 8830
start codons at 85, 95, 169, 222
confident = false
did not find CDR3
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1021 = GTCGTAAAGAAGGACA-1

using 19 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1022 = GTCGTAAAGAAGGGTA-1

using 45 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[4, 5, 9, 25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1027 = GTCGTAAAGAGTAAGG-1

using 60 reads

====================================================================================

graph has 62 edges initially, 4 edges after simplification

total ucounts = 10
nonsolo ucounts = 7[2, 4, 7^2, 11, 12, 14]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1029 = GTCGTAAAGATCGGGT-1

using 97 reads

====================================================================================

graph has 72 edges initially, 4 edges after simplification

total ucounts = 12
nonsolo ucounts = 7[4^3, 12, 14, 23, 31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1030 = GTCGTAAAGATGCCTT-1

using 281 reads

====================================================================================

graph has 300 edges initially, 10 edges after simplification

total ucounts = 33
nonsolo ucounts = 25[2^4, 3^2, 4, 5, 6, 7^3, 8, 9, 10^3, 11, 12, 13, 21, 24, 27, 30, 38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1037 = GTCGTAAAGCTGCCCA-1

using 61 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 7[2^2, 4, 6, 11, 14, 21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1039 = GTCGTAAAGGCGCTCT-1

using 38 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[5, 6, 25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1040 = GTCGTAAAGGCTAGCA-1

using 12160 reads

====================================================================================

graph has 7734 edges initially, 264 edges after simplification

total ucounts = 1025
nonsolo ucounts = 779[2^128, 3^76, 4^45, 5^51, 6^30, 7^30, 8^29, 9^22, 10^18, 11^32, 12^30, 13^22, 14^17, 15^25, 16^16, 17^20, 18^15, 19^8, 20^15, 21^13, 22^6, 23^10, 24^8, 25^6, 26^5, 27^5, 28^10, 29^7, 30^8, 31^6, 32^6, 33^11, 34^5, 35^5, 36^2, 37^3, 38^6, 39, 40, 41, 42^6, 43^3, 44, 45, 48^2, 50, 51, 53, 54, 55, 63^2, 105, 189, 300, 691, 1244]
surviving nonsolo ucounts = 6[18, 105, 189, 300, 691, 1244]
ids = [499, 494, 761, 221, 143, 121]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1042 = GTCGTAAAGGGTGTTG-1

using 1429 reads

====================================================================================

graph has 1434 edges initially, 64 edges after simplification

total ucounts = 233
nonsolo ucounts = 181[2^39, 3^18, 4^21, 5^13, 6^13, 7^11, 8^9, 9^7, 10^8, 11^2, 12^7, 13^3, 14^6, 15^2, 16^5, 17^2, 19^2, 20^3, 21^2, 22, 23, 24, 25^2, 31^2, 32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1048 = GTCGTAAAGTAGGCCA-1

using 46 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 6[2^2, 4, 7, 14, 15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1049 = GTCGTAAAGTATCGAA-1

using 28 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1050 = GTCGTAAAGTCAATAG-1

using 97 reads

====================================================================================

graph has 120 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 8[2, 3, 4, 7^2, 14, 21, 37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1058 = GTCGTAAAGTGTCCAT-1

using 76 reads

====================================================================================

graph has 62 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 5[2, 7^2, 16, 40]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1060 = GTCGTAAAGTTACCCA-1

using 102 reads

====================================================================================

graph has 98 edges initially, 2 edges after simplification

total ucounts = 13
nonsolo ucounts = 9[2, 3, 4^3, 11^2, 22, 37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1061 = GTCGTAAAGTTCGCGC-1

using 8139 reads

====================================================================================

graph has 5284 edges initially, 260 edges after simplification

total ucounts = 534
nonsolo ucounts = 416[2^58, 3^28, 4^19, 5^18, 6^17, 7^21, 8^16, 9^12, 10^11, 11^7, 12^11, 13^13, 14^12, 15^11, 16^3, 17^7, 18^7, 19^11, 20^11, 21^7, 22^5, 23^8, 24^7, 25^8, 26^3, 27^3, 28^3, 29^4, 30^3, 31, 32^2, 33^2, 34^7, 35^6, 36^3, 37^6, 38^4, 39^5, 40^6, 41, 42^2, 43^2, 44^2, 45^3, 46, 47, 48, 49, 50^2, 51, 52, 53^2, 54^2, 55, 57, 60, 63, 67, 74, 81, 1345]
surviving nonsolo ucounts = 1[1345]
ids = [351]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1064 = GTCGTAACAACACCTA-1

using 659 reads

====================================================================================

graph has 240 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[2^2, 4, 649]
surviving nonsolo ucounts = 1[649]
ids = [0]

====================================================================================

UMI info for barcode GTCGTAACAACACCTA-1 contig 1 = ATGTTCACAG...
umi AAGCATTCGT = 655 reads: +400 validated

GOOD CONTIGS

TIG 1[bases=504]
0-52 ==> 7-59 on |567|TRBV12-4|5'UTR| [len=59] (mis=0)
52-399 ==> 0-347 on |568|TRBV12-4|L-REGION+V-REGION| [len=347] (mis=5)
390-402 ==> 0-12 on |535|TRBD1|D-REGION| [len=12] (mis=3)
409-452 ==> 7-50 on |540|TRBJ1-5|J-REGION| [len=50] (mis=0)
452-504 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 1 umis using 40 reads
cdr3 = CASSQGAPGQPQHF at 382, score = 8 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 643
start codons at 0, 52, 110, 151, 174, 223, 226, 275, 286, 313, 320, 428
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1066 = GTCGTAACAAGTTGTC-1

using 24 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 3[3, 10, 11]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1077 = GTCGTAACACCTATCC-1

using 59 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 4[3, 8, 23, 24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1079 = GTCGTAACACTCGACG-1

using 22 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1080 = GTCGTAACAGATCTGT-1

using 69 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[32, 36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1085 = GTCGTAACAGGGATTG-1

using 24 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1092 = GTCGTAACATGTAGTC-1

using 8381 reads

====================================================================================

graph has 4955 edges initially, 184 edges after simplification

total ucounts = 575
nonsolo ucounts = 476[2^57, 3^32, 4^23, 5^20, 6^16, 7^18, 8^10, 9^11, 10^14, 11^11, 12^9, 13^11, 14^8, 15^17, 16^8, 17^7, 18^16, 19^8, 20^6, 21^12, 22^14, 23^8, 24^18, 25^7, 26^4, 27, 28^9, 29^10, 30^3, 31^5, 32^6, 33^7, 34^2, 35^4, 36^3, 37^3, 38^5, 39^6, 40^7, 42^5, 43^4, 44^4, 45^4, 46^3, 47^3, 48, 49, 50^2, 53, 54^2, 55^2, 56, 57, 60^2, 61, 62, 64, 65]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1093 = GTCGTAACATGTCTCC-1

using 1509 reads

====================================================================================

graph has 1648 edges initially, 16 edges after simplification

total ucounts = 342
nonsolo ucounts = 216[2^70, 3^50, 4^30, 5^17, 6^9, 7^14, 8^5, 9^2, 10^4, 11^2, 13, 14^3, 16^2, 19, 21, 39, 40, 52, 70, 288]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1095 = GTCGTAAGTAAAGTCA-1

using 45 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[45]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1097 = GTCGTAAGTAGGAGTC-1

using 22 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1107 = GTCGTAAGTCTCTTAT-1

using 47 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[47]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1108 = GTCGTAAGTCTGCCAG-1

using 51 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[51]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1113 = GTCGTAAGTGCTTCTC-1

using 59 reads

====================================================================================

graph has 56 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 5[2, 3^2, 8, 41]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1118 = GTCGTAAGTGTTCTTT-1

using 184 reads

====================================================================================

graph has 120 edges initially, 6 edges after simplification

total ucounts = 12
nonsolo ucounts = 11[5, 7, 8^2, 10, 12, 15, 23^2, 27, 45]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1120 = GTCGTAAGTTATTCTC-1

using 38 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1125 = GTCGTAAGTTTAGGAA-1

using 21 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1129 = GTCGTAATCACTGGGC-1

using 828 reads

====================================================================================

graph has 625 edges initially, 22 edges after simplification

total ucounts = 63
nonsolo ucounts = 50[2^3, 3^4, 4^3, 5^6, 6^3, 8, 9^2, 10^2, 11, 12, 14^2, 15^3, 16^2, 19^2, 24^2, 25^2, 27, 33^2, 36, 39, 40, 42^2, 44^2, 47]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1136 = GTCGTAATCCAATGGT-1

using 2377 reads

====================================================================================

graph has 2443 edges initially, 72 edges after simplification

total ucounts = 559
nonsolo ucounts = 328[2^114, 3^67, 4^39, 5^26, 6^14, 7^16, 8^7, 9^8, 10^2, 11^4, 12^3, 13^2, 14^2, 15^3, 16^3, 18^2, 21^2, 22^2, 24, 28, 30, 31^2, 32, 40, 49, 58, 63, 94, 258]
surviving nonsolo ucounts = 2[94, 258]
ids = [58, 159]

====================================================================================

REJECT CONTIGS

TIG 1[bases=382]
0-42 ==> 267-309 on |630|TRBV5-1|5'UTR| [len=309] (mis=0)
42-330 ==> 0-288 on |631|TRBV5-1|L-REGION+V-REGION| [len=343] (mis=0)
330-382 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
umis assigned: [58, 159]
of which 2 are surviving nonsolos
reads assigned: 272
start codons at 42, 324
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1141 = GTCGTAATCCGAATGT-1

using 35 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1144 = GTCGTAATCCTACAGA-1

using 107 reads

====================================================================================

graph has 56 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[107]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1151 = GTCGTAATCTACTATC-1

using 80 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[80]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1153 = GTCGTAATCTATCCCG-1

using 252 reads

====================================================================================

graph has 223 edges initially, 4 edges after simplification

total ucounts = 21
nonsolo ucounts = 17[4, 5^3, 7, 8, 9, 11^2, 13, 15, 16^2, 19, 25, 31, 48]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1159 = GTCGTAATCTCTTATG-1

using 31938 reads

====================================================================================

graph has 16993 edges initially, 629 edges after simplification

total ucounts = 2060
nonsolo ucounts = 1669[2^215, 3^146, 4^117, 5^107, 6^78, 7^51, 8^58, 9^62, 10^40, 11^43, 12^38, 13^31, 14^40, 15^40, 16^32, 17^21, 18^26, 19^21, 20^22, 21^19, 22^22, 23^15, 24^14, 25^14, 26^12, 27^17, 28^18, 29^12, 30^12, 31^6, 32^10, 33^14, 34^13, 35^6, 36^14, 37^11, 38^14, 39^15, 40^12, 41^6, 42^10, 43^8, 44^8, 45^9, 46^11, 47^12, 48^13, 49^10, 50^10, 51^7, 52^4, 53^8, 54^12, 55^4, 56^8, 57^6, 58, 59^3, 60^2, 61^8, 62^3, 63, 64^2, 65^2, 66^2, 67^4, 68^4, 69^2, 70^2, 71, 72^3, 73^5, 74^2, 75^2, 77^2, 78, 79, 83^3, 87, 88, 89, 94, 102, 118, 617, 1081, 1551]
surviving nonsolo ucounts = 3[617, 1081, 1551]
ids = [344, 827, 1082]

====================================================================================

UMI info for barcode GTCGTAATCTCTTATG-1 contig 1 = TGGGCTTTCT...
umi ATCTCTGAAA = 4 reads: -396 +2 -2XX invalidated
umi ATTTACCTGT = 613 reads: +400 validated
umi CTAGTGGATA = 1092 reads: +400 validated
umi CTCTTACCAT = 1 reads: -400X invalidated
umi GCACAAGTCC = 1542 reads: +400 validated
umi GCGGCTGCAG = 2 reads: -397X +2 -1XX invalidated

GOOD CONTIGS

TIG 1[bases=473]
21-365 ==> 0-344 on |613|TRBV28|L-REGION+V-REGION| [len=344] (mis=4)
371-421 ==> 0-50 on |540|TRBJ1-5|J-REGION| [len=50] (mis=3)
421-473 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 3 umis using 250 reads
cdr3 = CASSMQGAGNQPQHF at 348, score = 6 + 8
umis assigned: [282, 344, 827, 894, 1082, 1145]
of which 3 are surviving nonsolos
reads assigned: 3213
start codons at 21, 79, 143, 156, 163, 171, 226, 229, 237, 339, 360, 397
confident = true
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1162 = GTCGTAATCTGGCGTG-1

using 48 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[7, 41]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1171 = GTCTCGTAGATGCCAG-1

using 30 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1172 = GTCTCGTAGATGTTAG-1

using 53 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 12
nonsolo ucounts = 8[2^2, 3, 4^2, 5, 8, 21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1181 = GTCTCGTAGCTCAACT-1

using 203 reads

====================================================================================

graph has 206 edges initially, 4 edges after simplification

total ucounts = 31
nonsolo ucounts = 24[2^5, 3^2, 4, 5^2, 6^3, 7^3, 8^2, 11, 17, 19, 20, 22^2]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1182 = GTCTCGTAGCTGCGAA-1

using 29 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 5[2, 3^2, 4, 14]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1187 = GTCTCGTAGGCAAAGA-1

using 1526 reads

====================================================================================

graph has 936 edges initially, 20 edges after simplification

total ucounts = 331
nonsolo ucounts = 200[2^69, 3^40, 4^31, 5^21, 6^9, 7^5, 8^11, 9^2, 10^3, 11, 12^2, 13, 26, 49, 66, 187, 306]
surviving nonsolo ucounts = 3[5, 187, 306]
ids = [76, 257, 252]

====================================================================================

UMI info for barcode GTCTCGTAGGCAAAGA-1 contig 1 = AAAATGCCCC...
umi CACCTCCCTG = 5 reads: +70 -1 +19 -1 +11 -30 +56 -212 non-validated
umi TCCAACTTCG = 304 reads: +400 validated
umi TCCCGCGGTT = 187 reads: +400 validated

GOOD CONTIGS

TIG 1[bases=526]
0-74 ==> 0-74 on |591|TRBV2|5'UTR| [len=74] (mis=0)
74-421 ==> 0-347 on |592|TRBV2|L-REGION+V-REGION| [len=347] (mis=5)
426-474 ==> 0-48 on |536|TRBJ1-1|J-REGION| [len=48] (mis=3)
474-526 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 2 umis using 33 reads
cdr3 = CASRYQGRDTEAFF at 404, score = 6 + 8
umis assigned: [76, 252, 257]
of which 3 are surviving nonsolos
reads assigned: 490
start codons at 3, 31, 74, 91, 173, 285, 315, 342, 395
confident = true
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1189 = GTCTCGTAGGCCCTCA-1

using 38 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 14
nonsolo ucounts = 8[2, 3^2, 4^3, 5, 7]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1190 = GTCTCGTAGGCGATAC-1

using 178 reads

====================================================================================

graph has 110 edges initially, 2 edges after simplification

total ucounts = 18
nonsolo ucounts = 11[2^4, 4^3, 5, 6, 7, 133]
surviving nonsolo ucounts = 1[133]
ids = [0]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1197 = GTCTCGTAGTTCGCAT-1

using 24 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 4[3, 4, 5, 9]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1200 = GTCTCGTCAACGCACC-1

using 763 reads

====================================================================================

graph has 579 edges initially, 20 edges after simplification

total ucounts = 46
nonsolo ucounts = 36[2^3, 3^5, 4^4, 5^2, 6, 7^2, 8, 9^2, 12, 17^2, 18, 22, 26, 30, 34, 37, 42, 48, 51, 62, 71, 76, 97]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1201 = GTCTCGTCAACTGCGC-1

using 86 reads

====================================================================================

graph has 84 edges initially, 4 edges after simplification

total ucounts = 13
nonsolo ucounts = 12[2^3, 3, 4^2, 5^2, 10, 14, 17^2]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1202 = GTCTCGTCAACTGCTA-1

using 82 reads

====================================================================================

graph has 52 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[82]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1205 = GTCTCGTCAATCCGAT-1

using 260 reads

====================================================================================

graph has 232 edges initially, 6 edges after simplification

total ucounts = 45
nonsolo ucounts = 34[2^8, 3^3, 4^2, 5^5, 6^3, 7, 8, 9^3, 10^2, 13^2, 14, 15, 19, 37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1206 = GTCTCGTCAATGAAAC-1

using 69 reads

====================================================================================

graph has 68 edges initially, 4 edges after simplification

total ucounts = 15
nonsolo ucounts = 10[2^6, 8, 9, 13, 22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1207 = GTCTCGTCAATGCCAT-1

using 74 reads

====================================================================================

graph has 72 edges initially, 2 edges after simplification

total ucounts = 18
nonsolo ucounts = 15[2^6, 3^4, 5, 8, 9, 10, 15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1208 = GTCTCGTCAATGGATA-1

using 52 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[52]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1209 = GTCTCGTCAATGTTGC-1

using 102 reads

====================================================================================

graph has 92 edges initially, 6 edges after simplification

total ucounts = 25
nonsolo ucounts = 15[2^5, 3^2, 4, 5, 6^2, 8^2, 12, 27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1210 = GTCTCGTCAATTCCTT-1

using 85 reads

====================================================================================

graph has 44 edges initially, 4 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[84]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1211 = GTCTCGTCACAAGACG-1

using 53 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[53]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1212 = GTCTCGTCACACATGT-1

using 72 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[72]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1213 = GTCTCGTCACACCGAC-1

using 224 reads

====================================================================================

graph has 118 edges initially, 2 edges after simplification

total ucounts = 15
nonsolo ucounts = 13[2^2, 3, 4, 5^2, 6^2, 9, 10, 22, 67, 81]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1214 = GTCTCGTCACAGTCGC-1

using 77 reads

====================================================================================

graph has 46 edges initially, 6 edges after simplification

total ucounts = 16
nonsolo ucounts = 7[2, 6^2, 12^2, 13, 16]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1216 = GTCTCGTCACATTTCT-1

using 68 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[68]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1217 = GTCTCGTCACCAACCG-1

using 167 reads

====================================================================================

graph has 160 edges initially, 4 edges after simplification

total ucounts = 27
nonsolo ucounts = 22[2^6, 3^2, 4^3, 6, 7, 8, 9^2, 10^2, 11, 12, 17, 33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1218 = GTCTCGTCACCAGGCT-1

using 84 reads

====================================================================================

graph has 66 edges initially, 4 edges after simplification

total ucounts = 16
nonsolo ucounts = 12[2^5, 4, 7^2, 9, 10, 12, 21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1219 = GTCTCGTCACCAGGTC-1

using 804 reads

====================================================================================

graph has 230 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[804]
surviving nonsolo ucounts = 1[804]
ids = [0]

====================================================================================

UMI info for barcode GTCTCGTCACCAGGTC-1 contig 1 = AGGGAGCTGT...
umi TCTATATCCG = 804 reads: +400 validated

GOOD CONTIGS

TIG 1[bases=635]
0-193 ==> 32-225 on |520|TRAV8-2|5'UTR| [len=225] (mis=1)
193-534 ==> 0-341 on |521|TRAV8-2|L-REGION+V-REGION| [len=341] (mis=3)
532-593 ==> 0-61 on |427|TRAJ40|J-REGION| [len=61] (mis=2)
593-635 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 1 umis using 31 reads
cdr3 = CVVSVTSGTYKYIF at 520, score = 7 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 791
start codons at 193, 356, 496
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1220 = GTCTCGTCACCGAAAG-1

using 81 reads

====================================================================================

graph has 80 edges initially, 2 edges after simplification

total ucounts = 16
nonsolo ucounts = 11[2^2, 3^3, 4, 6, 11, 12, 13, 17]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1221 = GTCTCGTCACGACGAA-1

using 46 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[46]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1223 = GTCTCGTCACGTCAGC-1

using 99 reads

====================================================================================

graph has 74 edges initially, 2 edges after simplification

total ucounts = 18
nonsolo ucounts = 12[2, 3^2, 4, 6^2, 8^3, 9, 14, 22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1225 = GTCTCGTCAGACAGGT-1

using 100 reads

====================================================================================

graph has 48 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[100]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1226 = GTCTCGTCAGATCTGT-1

using 668 reads

====================================================================================

graph has 392 edges initially, 10 edges after simplification

total ucounts = 16
nonsolo ucounts = 11[6, 9, 11, 13, 24, 30, 35, 39, 50, 101, 345]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1228 = GTCTCGTCAGCATACT-1

using 96 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[96]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1229 = GTCTCGTCAGCCTTTC-1

using 3531 reads

====================================================================================

graph has 646 edges initially, 4 edges after simplification

total ucounts = 14
nonsolo ucounts = 10[2, 6, 7, 8, 10, 11^2, 15, 25, 3432]
surviving nonsolo ucounts = 1[3432]
ids = [12]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1230 = GTCTCGTCAGCGAACA-1

using 50 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[50]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1232 = GTCTCGTCAGCTGCTG-1

using 74 reads

====================================================================================

graph has 66 edges initially, 2 edges after simplification

total ucounts = 14
nonsolo ucounts = 10[3^2, 4^3, 5^2, 10, 16^2]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1233 = GTCTCGTCAGCTGTAT-1

using 114 reads

====================================================================================

graph has 64 edges initially, 2 edges after simplification

total ucounts = 17
nonsolo ucounts = 11[2^3, 3, 4, 5^4, 12, 63]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1234 = GTCTCGTCAGGATCGA-1

using 80 reads

====================================================================================

graph has 61 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 7[4, 6, 9^2, 12, 16, 21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1237 = GTCTCGTCAGTATCTG-1

using 76 reads

====================================================================================

graph has 99 edges initially, 2 edges after simplification

total ucounts = 16
nonsolo ucounts = 13[2^4, 3, 4, 6^2, 7^3, 11, 14]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1238 = GTCTCGTCAGTCGATT-1

using 200 reads

====================================================================================

graph has 158 edges initially, 4 edges after simplification

total ucounts = 17
nonsolo ucounts = 14[2, 3^3, 5, 9, 10^2, 11, 13, 15, 17, 27, 69]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1240 = GTCTCGTCATAAAGGT-1

using 44 reads

====================================================================================

graph has 48 edges initially, 8 edges after simplification

total ucounts = 17
nonsolo ucounts = 9[2^6, 4, 6, 14]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1242 = GTCTCGTCATACGCCG-1

using 60 reads

====================================================================================

graph has 56 edges initially, 4 edges after simplification

total ucounts = 12
nonsolo ucounts = 6[2, 3, 5, 7, 8, 29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1243 = GTCTCGTCATACTCTT-1

using 76 reads

====================================================================================

graph has 58 edges initially, 2 edges after simplification

total ucounts = 12
nonsolo ucounts = 10[2^2, 4, 5, 6, 8, 10^2, 12, 15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1244 = GTCTCGTCATATACCG-1

using 342 reads

====================================================================================

graph has 132 edges initially, 6 edges after simplification

total ucounts = 15
nonsolo ucounts = 10[2^2, 3, 4^2, 12^2, 16, 22, 260]
surviving nonsolo ucounts = 1[260]
ids = [6]

====================================================================================

UMI info for barcode GTCTCGTCATATACCG-1 contig 1 = ACAGTGATCC...
umi CGCATTCCAG = 255 reads: +397 validated

GOOD CONTIGS

TIG 1[bases=496]
0-47 ==> 0-47 on |674|TRBV7-2|5'UTR| [len=47] (mis=0)
47-394 ==> 0-347 on |675|TRBV7-2|L-REGION+V-REGION| [len=347] (mis=2)
397-444 ==> 0-47 on |550|TRBJ2-7|J-REGION| [len=47] (mis=3)
444-496 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 1 umis using 20 reads
cdr3 = CASSSGGLYEQYF at 377, score = 8 + 8
umis assigned: [6]
of which 1 are surviving nonsolos
reads assigned: 253
start codons at 47, 156
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1245 = GTCTCGTCATATGCTG-1

using 32 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1248 = GTCTCGTCATCTACGA-1

using 106 reads

====================================================================================

graph has 138 edges initially, 2 edges after simplification

total ucounts = 20
nonsolo ucounts = 16[2^2, 4^3, 5^4, 6, 7^2, 8, 9, 11, 18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1249 = GTCTCGTCATGACGGA-1

using 45 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 12
nonsolo ucounts = 10[2^2, 3^3, 4, 5, 7^3]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1250 = GTCTCGTCATGATCCA-1

using 112 reads

====================================================================================

graph has 74 edges initially, 2 edges after simplification

total ucounts = 22
nonsolo ucounts = 15[2^6, 4^2, 6, 7, 10, 12, 13, 16, 21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1252 = GTCTCGTCATGCTGGC-1

using 61 reads

====================================================================================

graph has 70 edges initially, 2 edges after simplification

total ucounts = 15
nonsolo ucounts = 10[2, 3^5, 5, 6, 13, 15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1253 = GTCTCGTCATGTTCCC-1

using 44 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[2, 42]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1255 = GTCTCGTCATTCCTCG-1

using 115 reads

====================================================================================

graph has 50 edges initially, 4 edges after simplification

total ucounts = 4
nonsolo ucounts = 4[2, 13, 41, 59]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1256 = GTCTCGTCATTCCTGC-1

using 81 reads

====================================================================================

graph has 80 edges initially, 4 edges after simplification

total ucounts = 15
nonsolo ucounts = 9[2^3, 3, 6, 9, 11, 13, 27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1257 = GTCTCGTCATTGGTAC-1

using 27 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1258 = GTCTCGTCATTTCACT-1

using 1023 reads

====================================================================================

graph has 364 edges initially, 6 edges after simplification

total ucounts = 23
nonsolo ucounts = 14[2, 3^2, 4, 6^3, 8^2, 9, 10, 13, 22, 914]
surviving nonsolo ucounts = 1[914]
ids = [13]

====================================================================================

UMI info for barcode GTCTCGTCATTTCACT-1 contig 1 = GAGAAGCCTC...
umi GTCTCCCAGC = 930 reads: +418 validated

GOOD CONTIGS

TIG 1[bases=577]
0-117 ==> 0-117 on |474|TRAV19|5'UTR| [len=117] (mis=1)
117-467 ==> 0-350 on |475|TRAV19|L-REGION+V-REGION| [len=350] (mis=5)
471-535 ==> 0-64 on |395|TRAJ10|J-REGION| [len=64] (mis=0)
535-577 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 1 umis using 24 reads
cdr3 = CALTPGDILTGGGNKLTF at 450, score = 7 + 8
umis assigned: [13]
of which 1 are surviving nonsolos
reads assigned: 905
start codons at 111, 117, 174, 229, 250, 340, 349
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1270 = GTCTCGTGTTTGTTGG-1

using 37 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1272 = GTCTCGTTCAATAAGG-1

using 430 reads

====================================================================================

graph has 262 edges initially, 8 edges after simplification

total ucounts = 28
nonsolo ucounts = 20[2^3, 3, 4, 5^2, 6, 7, 8^3, 13, 16, 19, 42, 50, 53, 80, 89]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1273 = GTCTCGTTCACATGCA-1

using 29 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1274 = GTCTCGTTCACGGTTA-1

using 56 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[56]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1275 = GTCTCGTTCACTTCAT-1

using 201 reads

====================================================================================

graph has 196 edges initially, 6 edges after simplification

total ucounts = 31
nonsolo ucounts = 21[2^7, 3^2, 4^4, 6, 7, 8^2, 9, 14, 18, 85]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1277 = GTCTCGTTCAGCGACC-1

using 93 reads

====================================================================================

graph has 81 edges initially, 4 edges after simplification

total ucounts = 16
nonsolo ucounts = 12[2^6, 3, 4, 8, 12, 19, 31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1278 = GTCTCGTTCAGGTTCA-1

using 45 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[45]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1280 = GTCTCGTTCATCGGAT-1

using 55 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[55]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1281 = GTCTCGTTCATGGTCA-1

using 28 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1282 = GTCTCGTTCATGTCTT-1

using 367 reads

====================================================================================

graph has 348 edges initially, 12 edges after simplification

total ucounts = 28
nonsolo ucounts = 25[2^3, 4^3, 5^6, 6, 7, 8^2, 9, 11, 13, 19, 31, 34, 51, 56, 63]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1284 = GTCTCGTTCCAAACAC-1

using 76 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[76]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1285 = GTCTCGTTCCAAACTG-1

using 58 reads

====================================================================================

graph has 52 edges initially, 2 edges after simplification

total ucounts = 14
nonsolo ucounts = 9[2^3, 3, 4, 5, 8, 13, 14]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1287 = GTCTCGTTCCAATGGT-1

using 86 reads

====================================================================================

graph has 104 edges initially, 4 edges after simplification

total ucounts = 18
nonsolo ucounts = 14[2^2, 3^4, 5^2, 7^2, 8, 9, 10, 15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1294 = GTCTCGTTCCTCAATT-1

using 54 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 14
nonsolo ucounts = 12[2^5, 3^4, 5, 6, 19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1295 = GTCTCGTTCCTGTAGA-1

using 79 reads

====================================================================================

graph has 56 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[79]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1297 = GTCTCGTTCGAATGCT-1

using 51 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 12
nonsolo ucounts = 3[2^2, 38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1301 = GTCTCGTTCGGAGGTA-1

using 471 reads

====================================================================================

graph has 336 edges initially, 14 edges after simplification

total ucounts = 24
nonsolo ucounts = 22[2, 3^4, 4^2, 5, 7, 8, 10, 13, 14, 15, 18, 19, 23, 25, 42, 81, 83, 84]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1303 = GTCTCGTTCGGCGGTT-1

using 130 reads

====================================================================================

graph has 114 edges initially, 4 edges after simplification

total ucounts = 9
nonsolo ucounts = 9[2, 5^2, 7, 10, 14, 17, 26, 44]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1305 = GTCTCGTTCGTCCAGG-1

using 73 reads

====================================================================================

graph has 88 edges initially, 2 edges after simplification

total ucounts = 13
nonsolo ucounts = 8[2^2, 3, 4^4, 45]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1308 = GTCTCGTTCTAAGCCA-1

using 31 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 15
nonsolo ucounts = 8[2^3, 3^2, 4^3]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1309 = GTCTCGTTCTCATTCA-1

using 31 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1311 = GTCTCGTTCTCGCATC-1

using 49 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[5, 44]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1313 = GTCTCGTTCTGTCAAG-1

using 34 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1315 = GTCTCGTTCTGTCTCG-1

using 233 reads

====================================================================================

graph has 102 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 5[5, 6, 8, 9, 203]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1316 = GTCTCGTTCTGTGCAA-1

using 100 reads

====================================================================================

graph has 117 edges initially, 6 edges after simplification

total ucounts = 13
nonsolo ucounts = 9[2, 4^2, 7, 8, 9, 13, 17, 32]
surviving nonsolo ucounts = 1[32]
ids = [12]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1320 = GTCTCGTTCTTGACGA-1

using 35 reads

====================================================================================

graph has 6 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1323 = GTCTCGTTCTTTACAC-1

using 29 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 5[2^2, 3^2, 14]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1324 = GTCTCGTTCTTTAGTC-1

using 65 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[65]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1325 = GTCTTCGAGAAGAAGC-1

using 24575 reads

====================================================================================

graph has 14057 edges initially, 584 edges after simplification

total ucounts = 953
nonsolo ucounts = 820[2^57, 3^41, 4^40, 5^45, 6^29, 7^24, 8^19, 9^16, 10^15, 11^16, 12^12, 13^11, 14^13, 15^15, 16^16, 17^15, 18^14, 19^15, 20^5, 21^13, 22^11, 23^10, 24^7, 25^5, 26^9, 27^10, 28^13, 29^6, 30^8, 31^14, 32^9, 33^6, 34^11, 35^3, 36^5, 37^11, 38^10, 39^5, 40^7, 41^4, 42, 43^9, 44^3, 45^4, 46^3, 47^7, 48^6, 49^5, 50^7, 51^8, 52^5, 53^8, 54^3, 55^3, 56^6, 57^3, 58^3, 59, 60^2, 61^3, 62^6, 63^7, 64^3, 65^7, 66^3, 67^2, 68^2, 69^7, 70^6, 71^4, 72^4, 73^3, 74^2, 75^4, 76^3, 77^2, 79^6, 80^2, 81^3, 82^2, 83^2, 84^3, 85^5, 86^2, 87^2, 88^2, 89, 90^5, 91^2, 92^3, 95^2, 96^3, 97^2, 98, 99^3, 100, 101^3, 103, 105^2, 106, 108, 110, 116, 117, 122, 127, 133, 136, 137, 142, 144]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1327 = GTCTTCGAGAAGGCCT-1

using 109 reads

====================================================================================

graph has 66 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 6[2^2, 3, 28, 34, 37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1328 = GTCTTCGAGAATCTCC-1

using 310 reads

====================================================================================

graph has 188 edges initially, 12 edges after simplification

total ucounts = 17
nonsolo ucounts = 15[2^2, 4, 6^4, 15, 23^2, 26, 28, 33, 58, 70]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1330 = GTCTTCGAGACCTAGG-1

using 28 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 5[2, 3, 4^2, 13]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1331 = GTCTTCGAGACGCAAC-1

using 45 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 5[2, 3^2, 6, 26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1332 = GTCTTCGAGACTTTCG-1

using 19650 reads

====================================================================================

graph has 10901 edges initially, 250 edges after simplification

total ucounts = 1300
nonsolo ucounts = 939[2^162, 3^104, 4^75, 5^78, 6^51, 7^47, 8^35, 9^40, 10^32, 11^28, 12^29, 13^30, 14^23, 15^14, 16^21, 17^24, 18^13, 19^10, 20^11, 21^12, 22^7, 23^6, 24^7, 25^6, 26^3, 27^4, 28^6, 29^8, 31^4, 32, 33^2, 34, 36^5, 37^4, 38, 39, 40^2, 41, 42^2, 43^4, 44, 45, 46^2, 47^2, 48, 50, 52, 53, 54, 58^2, 59, 61, 63, 64, 76, 77, 80, 81, 105, 106, 1507, 7989]
surviving nonsolo ucounts = 2[1507, 7989]
ids = [611, 1175]

====================================================================================

REJECT CONTIGS

TIG 1[bases=309]
13-84 ==> 0-71 on segment before TRBJ2-5 exon 1 [len=71] (mis=0)
84-132 ==> 0-48 on |548|TRBJ2-5|J-REGION| [len=48] (mis=0)
132-204 ==> 0-72 on segment before TRBJ2-6 exon 1 [len=72] (mis=0)
257-309 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
umis assigned: [673, 1175]
of which 1 are surviving nonsolos
reads assigned: 7907
start codons at 
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1334 = GTCTTCGAGAGGTAGA-1

using 17693 reads

====================================================================================

graph has 8861 edges initially, 303 edges after simplification

total ucounts = 1154
nonsolo ucounts = 783[2^149, 3^92, 4^64, 5^49, 6^44, 7^25, 8^35, 9^27, 10^16, 11^24, 12^17, 13^22, 14^11, 15^12, 16^12, 17^14, 18^9, 19^14, 20^7, 21^10, 22^13, 23^7, 24^6, 25^13, 26^3, 27, 28^6, 29^5, 30^5, 31^9, 32^4, 33, 34^4, 35^5, 36^6, 37, 38^2, 39^3, 40^3, 41, 43^3, 44, 45^3, 46^4, 47^2, 49, 50, 53^2, 54, 55, 56, 59^2, 61, 64, 66^2, 78, 90, 91, 93, 3344, 4960]
surviving nonsolo ucounts = 2[3344, 4960]
ids = [43, 642]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1335 = GTCTTCGAGAGTACCG-1

using 52 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[52]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1336 = GTCTTCGAGATAGCAT-1

using 95 reads

====================================================================================

graph has 76 edges initially, 4 edges after simplification

total ucounts = 12
nonsolo ucounts = 6[4^2, 8, 13, 18, 42]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 70.1340 = GTCTTCGAGCACAGGT-1

using 61 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[3^2, 53]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!
sorting bam, mem = 0.18
running samtools sort -l 8G -o /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_T_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk70-ucc6a7a72e6/files/contig_bam_sorted.bam /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_T_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk70-ucc6a7a72e6/files/contig_bam.bam

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79.965 seconds used processing barcodes, peak mem = 0.33
