[stdout]

starting vdj_asm_asm, mem = 0.01
available mem = 829.33 GB
/scratch/etanis/software/cellranger-6.1.2/lib/bin/cr_vdj martian assembly main /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_T_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk52-ucc6a7a72e6 /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_T_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk52-ucc6a7a72e6/files /scratch/etanis/ST-118/multi/dimitri-multi/journal/SC_MULTI_CS.SC_MULTI_CORE.MULTI_GEM_WELL_PROCESSOR.VDJ_T_GEM_WELL_PROCESSOR.SC_VDJ_CONTIG_ASSEMBLER.ASSEMBLE_VDJ.fork0.chnk52.ucc6a7a72e6
n50_n50_rpu = 2159

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.0 = GAACATCAGGCTAGAC-1

using 60958 reads

====================================================================================

graph has 18462 edges initially, 330 edges after simplification

total ucounts = 825
nonsolo ucounts = 595[2^83, 3^61, 4^28, 5^40, 6^20, 7^24, 8^21, 9^25, 10^14, 11^29, 12^21, 13^20, 14^8, 15^18, 16^6, 17^7, 18^17, 19^14, 20^3, 21^3, 22^4, 23^6, 24^5, 25^4, 26^3, 27^11, 28^5, 29^4, 30, 31^2, 32^2, 33^3, 34^3, 35^3, 37, 38^2, 40, 41^2, 43^2, 44, 46, 47, 49^3, 50, 51, 52, 53, 54^2, 55, 56, 57^3, 58, 60, 62, 65^2, 67^4, 68^2, 71^2, 72, 74, 77, 78^2, 82, 90, 92, 98, 111, 125^2, 269, 294, 456, 541, 662, 737, 1204, 1399, 1554, 1558, 1582, 1634, 1673, 1744, 1833, 1865, 1982, 2000, 2012, 2061, 2213, 2251, 2533, 2895, 3639, 5498, 6336]
surviving nonsolo ucounts = 27[269, 294, 456, 541, 662, 737, 1204, 1399, 1554, 1558, 1582, 1634, 1673, 1744, 1833, 1865, 1982, 2000, 2012, 2061, 2213, 2251, 2533, 2895, 3639, 5498, 6336]
ids = [745, 413, 739, 248, 772, 782, 264, 581, 309, 41, ...]

====================================================================================

UMI info for barcode GAACATCAGGCTAGAC-1 contig 1 = GATCTTAATT...
umi AAACTGTCCG = 2263 reads: +394 validated
umi AAGCTGTTTC = 1509 reads: +394 validated
umi ATCGCGGGCC = 1816 reads: +394 validated
umi ATTCCCCACT = 1998 reads: +394 validated
umi CAACCATTAA = 1991 reads: +394 validated
umi CATACCATAC = 2067 reads: +394 validated
umi CCACTTGTCA = 1979 reads: +394 validated
umi CTGGAATCGT = 272 reads: +394 validated
umi GCTTGCATGC = 3333 reads: -356X +2 -1XX +6 -4XX +5 -1XX +1 -2XX +4 -3XX +1 -3XX +5 invalidated
umi TCATACGACA = 1716 reads: +394 validated
umi TGTTACTATA = 5504 reads: -41X +1 -2XX +1 -4XX +345 invalidated

UMI info for barcode GAACATCAGGCTAGAC-1 contig 2 = GTTCCCCTAT...
umi AACCTAAGAG = 2228 reads: +391 validated
umi AAGGCATTGA = 1625 reads: +391 validated
umi ATGCAACCGG = 2896 reads: +391 validated
umi CACTCTTACT = 541 reads: +391 validated
umi CCCTCATACT = 1539 reads: +391 validated
umi CTTCAGTTTC = 1582 reads: +391 validated
umi GACGACTATG = 1828 reads: +391 validated
umi TACGTTTATA = 1366 reads: +391 validated
umi TCGATGGTTA = 2536 reads: +391 validated
umi TTAAATACAG = 450 reads: +391 validated
umi TTACACGTTA = 141 reads: -360 +5 -2X +1 -1X +5 -1X +6 -1XX +1 -1XX +5 -1XX +1 invalidated
umi TTGAGCGTCT = 739 reads: +391 validated

GOOD CONTIGS

TIG 1[bases=459]
0-23 ==> 0-23 on |463|TRAV13-1|5'UTR| [len=23] (mis=0)
23-360 ==> 0-337 on |464|TRAV13-1|L-REGION+V-REGION| [len=337] (mis=1)
355-417 ==> 0-62 on |415|TRAJ3|J-REGION| [len=62] (mis=5)
417-459 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 10 umis using 1339 reads
cdr3 = CAASKSSASKIIF at 347, score = 9 + 9
umis assigned: [10, 41, 180, 216, 232, 261, 289, 413, 493, 633] and 1 others
of which 11 are surviving nonsolos
reads assigned: 24119
start codons at 23, 78, 87, 237
confident = true

TIG 2[bases=500]
0-57 ==> 0-57 on |647|TRBV6-1|5'UTR| [len=57] (mis=0)
57-401 ==> 0-344 on |648|TRBV6-1|L-REGION+V-REGION| [len=344] (mis=4)
396-408 ==> 0-12 on |535|TRBD1|D-REGION| [len=12] (mis=3)
408-448 ==> 10-50 on |543|TRBJ2-1|J-REGION| [len=50] (mis=0)
448-500 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 11 umis using 1001 reads
cdr3 = CASSKTGDEQFF at 384, score = 9 + 8
umis assigned: [29, 45, 199, 248, 309, 424, 450, 581, 652, 739] and 2 others
of which 12 are surviving nonsolos
reads assigned: 17179
start codons at 15, 57, 115, 168, 192, 207, 234, 298, 307
confident = true

REJECT CONTIGS

TIG 1[bases=324]
5-200 ==> 5805-6000 on segment before TRBD1 exon 1 [len=6000] (mis=0)
222-272 ==> 0-50 on |540|TRBJ1-5|J-REGION| [len=50] (mis=5)
272-324 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
umis assigned: [72, 264, 509, 721, 772]
of which 4 are surviving nonsolos
reads assigned: 9564
start codons at 77, 87, 161, 248
confident = false
did not find CDR3
now this is a cell
paired!

ACCCAACCTGAAGACTCGGCTGTCTACTTCTGTGCAGCAAGTAAAAGCAGTGCTTCCAAGATAATCTTTGGATCAGGGACCAGACTCAGCATCCGGCCAA <==> GAGTCGGCTGCTCCCTCCCAGACATCTGTGTACTTCTGTGCCAGCAGCAAGACAGGGGACGAGCAGTTCTTCGGGCCAGGGACACGGCTCACCGTGCTAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.3 = GAACATCAGGTAGCTG-1

using 70 reads

====================================================================================

graph has 44 edges initially, 4 edges after simplification

total ucounts = 7
nonsolo ucounts = 3[2, 24, 40]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.5 = GAACATCAGTCAAGGC-1

using 287 reads

====================================================================================

graph has 160 edges initially, 6 edges after simplification

total ucounts = 13
nonsolo ucounts = 9[3, 5, 6, 8, 34, 46, 49, 62, 70]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.6 = GAACATCAGTCCAGGA-1

using 65 reads

====================================================================================

graph has 58 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[65]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.7 = GAACATCAGTCTCCTC-1

using 51 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[2, 49]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.11 = GAACATCAGTGGAGTC-1

using 39 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[3, 6, 28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.13 = GAACATCAGTGTGAAT-1

using 106 reads

====================================================================================

graph has 60 edges initially, 4 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[4, 27, 29, 44]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.15 = GAACATCAGTTATCGC-1

using 823 reads

====================================================================================

graph has 456 edges initially, 22 edges after simplification

total ucounts = 26
nonsolo ucounts = 25[2^2, 4, 8^3, 10, 11, 15, 16, 18, 21, 23^2, 25, 26, 28, 39, 45, 54, 66^2, 99, 102, 103]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.18 = GAACATCAGTTGTAGA-1

using 117 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[117]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.21 = GAACATCCAAGCTGGA-1

using 38 reads

====================================================================================

graph has 55 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 4[2^2, 7, 23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.22 = GAACATCCAAGGACTG-1

using 57 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[57]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.23 = GAACATCCAAGTACCT-1

using 55 reads

====================================================================================

graph has 54 edges initially, 2 edges after simplification

total ucounts = 14
nonsolo ucounts = 7[2, 3^2, 6, 10, 11, 13]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.24 = GAACATCCAATCGGTT-1

using 166 reads

====================================================================================

graph has 92 edges initially, 4 edges after simplification

total ucounts = 10
nonsolo ucounts = 6[2, 5, 12, 15, 43, 85]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.25 = GAACATCCAATGAAAC-1

using 57 reads

====================================================================================

graph has 56 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 7[3^5, 7, 33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.27 = GAACATCCACAGCGTC-1

using 54 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 1[52]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.28 = GAACATCCACATAACC-1

using 52 reads

====================================================================================

graph has 30 edges initially, 4 edges after simplification

total ucounts = 5
nonsolo ucounts = 4[2, 12, 18, 19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.29 = GAACATCCACATCCGG-1

using 43 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[43]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.33 = GAACATCCACCAGTTA-1

using 79 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[79]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.35 = GAACATCCACCTGGTG-1

using 50 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[49]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.37 = GAACATCCACGGTAGA-1

using 44 reads

====================================================================================

graph has 68 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[44]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.39 = GAACATCCACGTCTCT-1

using 207 reads

====================================================================================

graph has 90 edges initially, 6 edges after simplification

total ucounts = 3
nonsolo ucounts = 3[50, 57, 100]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.40 = GAACATCCACTATCTT-1

using 30 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.41 = GAACATCCACTTCTGC-1

using 64 reads

====================================================================================

graph has 74 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 7[2, 3, 6^2, 10, 11, 22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.44 = GAACATCCAGCTGCTG-1

using 57 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[57]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.48 = GAACATCCAGCTTCGG-1

using 15656 reads

====================================================================================

graph has 13448 edges initially, 510 edges after simplification

total ucounts = 1706
nonsolo ucounts = 1223[2^180, 3^134, 4^102, 5^93, 6^76, 7^58, 8^52, 9^53, 10^34, 11^40, 12^29, 13^31, 14^24, 15^23, 16^20, 17^18, 18^33, 19^20, 20^19, 21^12, 22^4, 23^13, 24^10, 25^7, 26^7, 27^7, 28^7, 29^10, 30^6, 31^4, 32^5, 33, 34^3, 35^4, 36^3, 37^3, 38, 39^4, 40^4, 41^2, 42^3, 43^5, 44^3, 45, 46^3, 47, 48^3, 50^3, 51^3, 52^3, 53, 55^2, 57, 59^4, 61, 62, 64^4, 66, 70, 71^3, 72, 73, 74^2, 75^2, 77, 79, 83, 87^2, 89, 90, 92, 93, 96^2, 118^2, 148]
surviving nonsolo ucounts = 8[2^2, 3^2, 4, 5, 13, 15]
ids = [230, 988, 1146, 1540, 800, 351, 677, 1465]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.50 = GAACATCCAGGATTGG-1

using 118 reads

====================================================================================

graph has 90 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[117]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.51 = GAACATCCAGGCGATA-1

using 92 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[92]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.52 = GAACATCCAGGCTGAA-1

using 27 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 2[5, 19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.53 = GAACATCCAGGTCTCG-1

using 218 reads

====================================================================================

graph has 106 edges initially, 6 edges after simplification

total ucounts = 6
nonsolo ucounts = 6[3, 9, 11, 29, 69, 97]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.55 = GAACATCCAGTCAGCC-1

using 56 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 1[52]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.56 = GAACATCCAGTCTTCC-1

using 113 reads

====================================================================================

graph has 54 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[113]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.58 = GAACATCCATAAGACA-1

using 120 reads

====================================================================================

graph has 68 edges initially, 4 edges after simplification

total ucounts = 5
nonsolo ucounts = 4[8, 12, 49, 50]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.63 = GAACATCCATGCTGGC-1

using 72 reads

====================================================================================

graph has 34 edges initially, 4 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[7, 27, 37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.64 = GAACATCCATGTAAGA-1

using 30 reads

====================================================================================

graph has 6 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.65 = GAACATCCATGTCGAT-1

using 17 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[8, 9]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.73 = GAACATCGTAGCTAAA-1

using 53 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 2[6, 44]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.76 = GAACATCGTCATACTG-1

using 85 reads

====================================================================================

graph has 100 edges initially, 2 edges after simplification

total ucounts = 12
nonsolo ucounts = 11[3^4, 4^2, 5, 13^2, 14, 19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.77 = GAACATCGTCATTAGC-1

using 26 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.83 = GAACATCGTCTCCCTA-1

using 18 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.85 = GAACATCGTCTCTTTA-1

using 36 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.91 = GAACATCGTGCCTGCA-1

using 102 reads

====================================================================================

graph has 62 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[2, 4, 10, 84]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.94 = GAACATCGTTCAGACT-1

using 2256 reads

====================================================================================

graph has 715 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 4[2, 12, 14, 2225]
surviving nonsolo ucounts = 1[2225]
ids = [5]

====================================================================================

UMI info for barcode GAACATCGTTCAGACT-1 contig 1 = CCTTTCATCA...
umi GCTAACAGGG = 2231 reads: +400 validated

GOOD CONTIGS

TIG 1[bases=504]
0-52 ==> 14-66 on |657|TRBV6-5|5'UTR| [len=66] (mis=0)
52-396 ==> 0-344 on |658|TRBV6-5|L-REGION+V-REGION| [len=344] (mis=2)
404-452 ==> 0-48 on |537|TRBJ1-2|J-REGION| [len=48] (mis=4)
452-504 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 1 umis using 143 reads
cdr3 = CASSYRAVRGYGYTF at 379, score = 9 + 9
umis assigned: [5]
of which 1 are surviving nonsolos
reads assigned: 2195
start codons at 10, 38, 52, 110, 163, 187, 194, 202, 229, 293, 302, 410
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.97 = GAACATCGTTCTCATT-1

using 19673 reads

====================================================================================

graph has 6840 edges initially, 128 edges after simplification

total ucounts = 492
nonsolo ucounts = 350[2^56, 3^35, 4^37, 5^24, 6^20, 7^12, 8^13, 9^11, 10^15, 11^17, 12^12, 13^6, 14^8, 15^8, 16^4, 17^13, 18^4, 19^3, 20^3, 21^3, 22^4, 23^2, 24^3, 25^3, 26, 28^2, 29, 31^2, 33, 34, 35, 36, 38, 39, 40, 45^2, 47, 48, 49, 50, 55^2, 56, 58, 60, 70, 82, 121, 214, 294, 851, 1798, 1946, 5195, 5463]
surviving nonsolo ucounts = 6[214, 294, 851, 1946, 5195, 5463]
ids = [486, 193, 165, 45, 73, 206]

====================================================================================

UMI info for barcode GAACATCGTTCTCATT-1 contig 1 = ATTCTTTCTT...
umi ACTATCACTG = 1929 reads: +403 validated

GOOD CONTIGS

TIG 1[bases=440]
0-20 ==> 0-20 on |612|TRBV28|5'UTR| [len=20] (mis=0)
20-364 ==> 0-344 on |613|TRBV28|L-REGION+V-REGION| [len=344] (mis=1)
372-423 ==> 0-51 on |539|TRBJ1-4|J-REGION| [len=51] (mis=5)
junction support: 1 umis using 143 reads
cdr3 = CASSLFGQAGDEKLFF at 347, score = 6 + 8
umis assigned: [45]
of which 1 are surviving nonsolos
reads assigned: 1904
start codons at 20, 78, 142, 155, 162, 170, 225, 228, 236, 338, 378
confident = true

REJECT CONTIGS

TIG 1[bases=508]
8-405 ==> 0-397 on |516|TRAV6|L-REGION+V-REGION| [len=397] (mis=5)
404-466 ==> 0-62 on |415|TRAJ3|J-REGION| [len=62] (mis=0)
466-508 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
umis assigned: [73, 165, 486]
of which 3 are surviving nonsolos
reads assigned: 6180
start codons at 8, 65, 282
confident = false
frameshifted full length transcript of length 508
did not find CDR3

TIG 2[bases=357]
5-251 ==> 5754-6000 on segment before TRBD1 exon 1 [len=6000] (mis=0)
263-305 ==> 6-48 on |537|TRBJ1-2|J-REGION| [len=48] (mis=0)
305-357 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
umis assigned: [193, 206, 248]
of which 2 are surviving nonsolos
reads assigned: 6493
start codons at 128, 138, 212, 263
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.99 = GAACATCGTTGGTAAA-1

using 133 reads

====================================================================================

graph has 146 edges initially, 2 edges after simplification

total ucounts = 18
nonsolo ucounts = 10[2^2, 3^2, 4, 6, 9, 15, 23, 58]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.100 = GAACATCGTTTGACTG-1

using 19 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.103 = GAACATCTCAACACCA-1

using 42 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[42]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.104 = GAACATCTCAAGCCTA-1

using 83 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 4[2^2, 3, 76]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.105 = GAACATCTCAATAAGG-1

using 40 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[40]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.106 = GAACATCTCAATCTCT-1

using 77 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[76]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.108 = GAACATCTCACGAAGG-1

using 61 reads

====================================================================================

graph has 90 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 5[2, 3, 7, 23, 24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.113 = GAACATCTCATGGTCA-1

using 67 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[67]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.114 = GAACATCTCCCACTTG-1

using 33 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.120 = GAACATCTCCGCGTTT-1

using 16 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[16]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.123 = GAACATCTCGCTTAGA-1

using 33 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 1[30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.124 = GAACATCTCGGACAAG-1

using 66 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[66]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.129 = GAACATCTCGTCCAGG-1

using 171 reads

====================================================================================

graph has 180 edges initially, 6 edges after simplification

total ucounts = 14
nonsolo ucounts = 12[2, 3, 4, 6, 7, 8, 10, 17, 18^2, 30, 46]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.135 = GAACATCTCTCGTATT-1

using 79 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[79]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.136 = GAACATCTCTCGTTTA-1

using 75 reads

====================================================================================

graph has 72 edges initially, 4 edges after simplification

total ucounts = 9
nonsolo ucounts = 8[2^2, 3, 10, 12, 13, 14, 18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.138 = GAACATCTCTGAAAGA-1

using 111 reads

====================================================================================

graph has 78 edges initially, 4 edges after simplification

total ucounts = 7
nonsolo ucounts = 7[2, 3, 5, 7, 12, 23, 59]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.139 = GAACATCTCTGAGGGA-1

using 1680 reads

====================================================================================

graph has 622 edges initially, 2 edges after simplification

total ucounts = 13
nonsolo ucounts = 7[2^3, 3, 5, 6, 1654]
surviving nonsolo ucounts = 1[1654]
ids = [6]

====================================================================================

REJECT CONTIGS

TIG 1[bases=422]
0-29 ==> 0-29 on |478|TRAV20|5'UTR| [len=69] (mis=0)
28-313 ==> 52-337 on |479|TRAV20|L-REGION+V-REGION| [len=337] (mis=4)
325-380 ==> 8-63 on |401|TRAJ17|J-REGION| [len=63] (mis=0)
380-422 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
umis assigned: [6]
of which 1 are surviving nonsolos
reads assigned: 1525
start codons at 
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.140 = GAACATCTCTGATACG-1

using 105 reads

====================================================================================

graph has 92 edges initially, 4 edges after simplification

total ucounts = 8
nonsolo ucounts = 7[2^2, 3, 12^2, 35, 38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.142 = GAACATCTCTTCAACT-1

using 76 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[76]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.143 = GAACATCTCTTCCTTC-1

using 73 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[73]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.145 = GAACCTAAGAAGATTC-1

using 2170 reads

====================================================================================

graph has 690 edges initially, 4 edges after simplification

total ucounts = 5
nonsolo ucounts = 4[2, 5, 29, 2133]
surviving nonsolo ucounts = 1[2133]
ids = [2]

====================================================================================

UMI info for barcode GAACCTAAGAAGATTC-1 contig 1 = TGGGGAGATA...
umi ATGTGGCAGC = 2142 reads: +391 validated

GOOD CONTIGS

TIG 1[bases=482]
49-385 ==> 0-336 on |481|TRAV21|L-REGION+V-REGION| [len=336] (mis=2)
381-440 ==> 0-59 on |422|TRAJ36|J-REGION| [len=59] (mis=5)
440-482 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 1 umis using 115 reads
cdr3 = CAVKDGANNLFF at 373, score = 9 + 9
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 2102
start codons at 49, 93, 293, 386
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.146 = GAACCTAAGAATTGTG-1

using 38 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.147 = GAACCTAAGACAAGCC-1

using 85 reads

====================================================================================

graph has 90 edges initially, 2 edges after simplification

total ucounts = 13
nonsolo ucounts = 8[2, 3, 6, 8, 9, 15, 16, 21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.148 = GAACCTAAGACTAAGT-1

using 48 reads

====================================================================================

graph has 6 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[48]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.150 = GAACCTAAGACTTTCG-1

using 58 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[58]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.152 = GAACCTAAGAGACTTA-1

using 20 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.157 = GAACCTAAGATGTGGC-1

using 65 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[65]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.160 = GAACCTAAGCCCAGCT-1

using 887 reads

====================================================================================

graph has 648 edges initially, 14 edges after simplification

total ucounts = 32
nonsolo ucounts = 28[2^3, 5, 6^2, 7^3, 9^2, 11, 16, 17^2, 21, 23, 25, 28, 32, 41, 58, 63, 66, 84, 91, 110, 118]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.163 = GAACCTAAGCGTTTAC-1

using 81 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[81]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.164 = GAACCTAAGCTAACAA-1

using 21 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.165 = GAACCTAAGCTCCTCT-1

using 56 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 6[2^4, 5, 41]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.166 = GAACCTAAGCTCCTTC-1

using 33885 reads

====================================================================================

graph has 11239 edges initially, 222 edges after simplification

total ucounts = 773
nonsolo ucounts = 575[2^79, 3^55, 4^39, 5^38, 6^25, 7^19, 8^26, 9^22, 10^18, 11^21, 12^22, 13^15, 14^16, 15^13, 16^7, 17^15, 18^13, 19^8, 20^10, 21^2, 22^11, 23^9, 24^9, 25^9, 26^4, 27^4, 28^5, 29^6, 30, 31^3, 32^5, 34^3, 35^5, 36, 37^4, 40, 41, 42^2, 43^2, 45, 47^2, 48, 49^3, 51, 52, 61, 62, 63, 82, 94, 122, 145, 890, 898, 900, 915, 1019, 1109, 1231, 1450, 1766, 7460, 8930]
surviving nonsolo ucounts = 12[19, 890, 898, 900, 915, 1019, 1109, 1231, 1450, 1766, 7460, 8930]
ids = [363, 764, 111, 530, 380, 750, 424, 156, 511, 501, ...]

====================================================================================

UMI info for barcode GAACCTAAGCTCCTTC-1 contig 1 = GAGAAGCCTC...
umi GCACATGATC = 1117 reads: +409 validated
umi TTGTAATCCA = 1019 reads: +409 validated

UMI info for barcode GAACCTAAGCTCCTTC-1 contig 2 = ACTCCCACCT...
umi AGAGGTTGCA = 851 reads: +415 validated
umi AGCAAAGGTG = 6937 reads: -341X +1 -1XX +2 -11XX +2 -1XX +3 -2XX +1 -1XX +1 -7XX +41 invalidated
umi ATATGTGTCT = 1239 reads: +415 validated
umi GAAAATGGAC = 896 reads: +403 -1 +11 non-validated
umi GTCAACTCAT = 1784 reads: +415 validated
umi GTCCTGCTCT = 1455 reads: +415 validated
umi GTGTTTCGTG = 896 reads: +415 validated
umi TAAGTCTCCG = 5692 reads: -341X +1 -1XX +2 -11XX +2 -1XX +3 -2XX +1 -1XX +1 -7XX +41 invalidated
umi TTTCTGGGCA = 845 reads: -400X +2 -11XX +2 invalidated

GOOD CONTIGS

TIG 1[bases=568]
0-117 ==> 0-117 on |474|TRAV19|5'UTR| [len=117] (mis=1)
117-467 ==> 0-350 on |475|TRAV19|L-REGION+V-REGION| [len=350] (mis=4)
463-526 ==> 0-63 on |431|TRAJ44|J-REGION| [len=63] (mis=2)
526-568 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 2 umis using 56 reads
cdr3 = CALSKYTGTASKLTF at 450, score = 7 + 9
umis assigned: [424, 750]
of which 2 are surviving nonsolos
reads assigned: 2107
start codons at 111, 117, 174, 229, 250, 340, 349
confident = true

TIG 2[bases=544]
0-77 ==> 0-77 on |578|TRBV15|5'UTR| [len=77] (mis=0)
77-421 ==> 0-344 on |579|TRBV15|L-REGION+V-REGION| [len=344] (mis=1)
434-450 ==> 0-16 on |730|TRBD2|D-REGION| [len=16] (mis=2)
451-492 ==> 9-50 on |543|TRBJ2-1|J-REGION| [len=50] (mis=0)
492-544 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 5 umis using 213 reads
cdr3 = CATSRGAAGRVTSGRLEQFF at 404, score = 6 + 8
umis assigned: [111, 116, 156, 380, 501, 511, 530, 557, 764]
of which 9 are surviving nonsolos
reads assigned: 20165
start codons at 43, 58, 77, 101, 129, 135, 140, 227, 282, 300, 395
confident = true
now this is a cell
paired!

GTCGTGGACTCAGCAGTATACTTCTGTGCTCTGAGTAAATATACCGGCACTGCCAGTAAACTCACCTTTGGGACTGGAACAAGACTTCAGGTCACGCTCG <==> GCCATGTACCTGTGTGCCACCAGCAGAGGCGCAGCAGGGAGGGTGACTAGCGGGAGGCTTGAGCAGTTCTTCGGGCCAGGGACACGGCTCACCGTGCTAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.169 = GAACCTAAGGACCACA-1

using 69 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[69]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.172 = GAACCTAAGGATATAC-1

using 103 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[103]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.173 = GAACCTAAGGATCGCA-1

using 54 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 2[2, 49]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.176 = GAACCTAAGGCTATCT-1

using 44 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[44]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.178 = GAACCTAAGGGATACC-1

using 77 reads

====================================================================================

graph has 88 edges initially, 4 edges after simplification

total ucounts = 7
nonsolo ucounts = 4[5^2, 30, 34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.182 = GAACCTAAGTACGTAA-1

using 150 reads

====================================================================================

graph has 128 edges initially, 6 edges after simplification

total ucounts = 21
nonsolo ucounts = 15[2^3, 3^3, 4, 5, 7^4, 27, 30, 35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.184 = GAACCTAAGTCAAGGC-1

using 53 reads

====================================================================================

graph has 68 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 4[3^2, 23, 24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.189 = GAACCTAAGTGAATTG-1

using 35 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[7, 28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.196 = GAACCTAAGTTAGCGG-1

using 23 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.200 = GAACCTACAAACGCGA-1

using 40 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[2, 3, 11, 22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.202 = GAACCTACAACTGCGC-1

using 70 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 1[68]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.203 = GAACCTACAAGCCGCT-1

using 49 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[49]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.208 = GAACCTACAATGGAGC-1

using 51 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[51]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.211 = GAACCTACACACGCTG-1

using 30 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.214 = GAACCTACACCAGGTC-1

using 18 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.215 = GAACCTACACCAGTTA-1

using 18 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.219 = GAACCTACACCGTTGG-1

using 73 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[73]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.220 = GAACCTACACGGACAA-1

using 35 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[4, 14, 16]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.223 = GAACCTACACGGTAAG-1

using 238 reads

====================================================================================

graph has 211 edges initially, 6 edges after simplification

total ucounts = 32
nonsolo ucounts = 16[2^2, 3^2, 6, 7, 8^3, 9^2, 13, 19, 23, 46, 56]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.225 = GAACCTACACTTCTGC-1

using 79 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[78]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.226 = GAACCTACAGACAAAT-1

using 73 reads

====================================================================================

graph has 94 edges initially, 4 edges after simplification

total ucounts = 9
nonsolo ucounts = 6[2^2, 9, 16, 19, 22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.227 = GAACCTACAGACACTT-1

using 118 reads

====================================================================================

graph has 72 edges initially, 4 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[19, 99]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.228 = GAACCTACAGACAGGT-1

using 30446 reads

====================================================================================

graph has 11072 edges initially, 180 edges after simplification

total ucounts = 598
nonsolo ucounts = 434[2^68, 3^36, 4^25, 5^18, 6^23, 7^22, 8^19, 9^15, 10^6, 11^14, 12^15, 13^6, 14^11, 15^9, 16^9, 17^7, 18^9, 19^8, 20^5, 21^7, 22^10, 23^2, 24^6, 25^5, 26^6, 27^3, 28^2, 29^3, 30^5, 31^4, 32^5, 33^2, 34^2, 35^2, 36^2, 38^3, 40^2, 41^2, 42, 43, 44^4, 49, 52, 53, 54, 56, 57^2, 72, 74, 77, 84, 96, 285, 396, 401, 438, 586, 597, 637, 749, 770, 835, 848, 977, 1052, 1071, 1185, 2089, 4401, 7424]
surviving nonsolo ucounts = 16[43, 438, 586, 597, 637, 749, 770, 835, 848, 977, 1052, 1071, 1185, 2089, 4401, 7424]
ids = [58, 585, 212, 184, 537, 348, 464, 220, 326, 211, ...]

====================================================================================

UMI info for barcode GAACCTACAGACAGGT-1 contig 1 = GTCCTGTCTC...
umi AGAGTAGGAC = 2083 reads: +397 validated
umi CATTCTTCAG = 428 reads: +49 -11XX +3 -4XX +259 -1 +8 -62 invalidated
umi CCCCTTGATA = 1075 reads: +397 validated
umi CCGTTCAGGT = 987 reads: +360 -31 +6 non-validated
umi CCTAGCGTTG = 579 reads: +370 -21 +6 non-validated
umi CGACAACCTC = 823 reads: +336 -1 +17 -43 non-validated
umi GCCCTCTTCC = 848 reads: +397 validated
umi GCGTGATTTC = 1060 reads: +397 validated
umi GGCAAGGACG = 744 reads: +397 validated
umi TCCAGTGGAG = 1179 reads: +397 validated
umi TGGTTATAGT = 626 reads: +348 -44 +5 non-validated

GOOD CONTIGS

TIG 1[bases=632]
0-183 ==> 25-208 on |587|TRBV19|5'UTR| [len=208] (mis=0)
183-520 ==> 0-337 on |588|TRBV19|L-REGION+V-REGION| [len=344] (mis=0)
532-580 ==> 0-48 on |537|TRBJ1-2|J-REGION| [len=48] (mis=5)
580-632 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 5 umis using 229 reads
cdr3 = CASTPGTQGYGYTF at 510, score = 6 + 9
umis assigned: [69, 184, 199, 211, 212, 220, 326, 336, 348, 475] and 1 others
of which 11 are surviving nonsolos
reads assigned: 10251
start codons at 183, 241, 292, 328, 333, 397, 538
confident = true

REJECT CONTIGS

TIG 1[bases=394]
37-141 ==> 0-104 on segment before TRBJ2-3 exon 1 [len=104] (mis=0)
141-190 ==> 0-49 on |546|TRBJ2-3|J-REGION| [len=49] (mis=0)
190-292 ==> 0-102 on segment before TRBJ2-4 exon 1 [len=102] (mis=0)
342-394 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
umis assigned: [89, 208, 513, 585]
of which 3 are surviving nonsolos
reads assigned: 12017
start codons at 51
confident = false
did not find CDR3
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.230 = GAACCTACAGCGATCC-1

using 73 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[11, 62]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.231 = GAACCTACAGCGTAAG-1

using 51 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[51]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.240 = GAACCTACATACTCTT-1

using 5053 reads

====================================================================================

graph has 3157 edges initially, 122 edges after simplification

total ucounts = 423
nonsolo ucounts = 311[2^32, 3^22, 4^15, 5^20, 6^18, 7^11, 8^18, 9^13, 10^14, 11^11, 12^20, 13^4, 14^8, 15^16, 16^7, 17^7, 18^5, 19^7, 20^10, 21^5, 22^4, 23^3, 24^3, 25, 26^5, 27^3, 28^5, 29, 30^2, 31, 32, 35, 37, 38, 41, 43, 44^2, 47, 50, 52, 53^2, 54, 80, 84, 90, 118, 350, 471]
surviving nonsolo ucounts = 1[471]
ids = [410]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.241 = GAACCTACATAGTAAG-1

using 159 reads

====================================================================================

graph has 76 edges initially, 6 edges after simplification

total ucounts = 4
nonsolo ucounts = 4[11, 31, 33, 84]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.242 = GAACCTACATCACGTA-1

using 188 reads

====================================================================================

graph has 92 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[188]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.243 = GAACCTACATCCGTGG-1

using 51 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[51]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.245 = GAACCTACATCTACGA-1

using 98 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[98]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.251 = GAACCTACATTGTGCA-1

using 51 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[51]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.252 = GAACCTAGTAAATGAC-1

using 17 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[17]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.253 = GAACCTAGTAACGCGA-1

using 63 reads

====================================================================================

graph has 52 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 4[2, 5, 21, 35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.254 = GAACCTAGTACCGGCT-1

using 111 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[111]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.255 = GAACCTAGTACTCGCG-1

using 36 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[2, 32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.256 = GAACCTAGTACTCTCC-1

using 46 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[45]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.260 = GAACCTAGTAGCAAAT-1

using 33 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.263 = GAACCTAGTCAACATC-1

using 27 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[5, 21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.267 = GAACCTAGTCCAGTAT-1

using 41 reads

====================================================================================

graph has 48 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 8[2^3, 3^2, 6, 9, 13]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.268 = GAACCTAGTCCATCCT-1

using 21805 reads

====================================================================================

graph has 9469 edges initially, 249 edges after simplification

total ucounts = 1006
nonsolo ucounts = 737[2^134, 3^71, 4^69, 5^51, 6^40, 7^38, 8^33, 9^29, 10^28, 11^26, 12^23, 13^18, 14^12, 15^18, 16^10, 17^13, 18^12, 19^7, 20^7, 21^5, 22^6, 23^4, 24^4, 25^6, 26^7, 27^5, 28^4, 29^3, 30^2, 31^3, 32^5, 33^6, 34^4, 35, 36^2, 38, 39^3, 40^2, 42, 43^2, 44, 45^2, 46, 47^3, 50^2, 51^2, 52, 53, 54, 58^2, 60, 61, 75, 1137, 5901, 6869]
surviving nonsolo ucounts = 3[1137, 5901, 6869]
ids = [794, 362, 612]

====================================================================================

UMI info for barcode GAACCTAGTCCATCCT-1 contig 1 = CAGTAACTTT...
umi TCCGCGGTCA = 1134 reads: +406 validated

GOOD CONTIGS

TIG 1[bases=547]
0-99 ==> 18-117 on |474|TRAV19|5'UTR| [len=117] (mis=0)
99-449 ==> 0-350 on |475|TRAV19|L-REGION+V-REGION| [len=350] (mis=3)
449-505 ==> 8-64 on |395|TRAJ10|J-REGION| [len=64] (mis=0)
505-547 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 1 umis using 48 reads
cdr3 = CALSDLGGGNKLTF at 432, score = 7 + 8
umis assigned: [794]
of which 1 are surviving nonsolos
reads assigned: 1125
start codons at 93, 99, 156, 211, 232, 322, 331
confident = false

REJECT CONTIGS

TIG 1[bases=375]
30-57 ==> 272-299 on |566|TRBV12-3|L-REGION+V-REGION| [len=347] (mis=1)
40-93 ==> 282-335 on |564|TRBV11-3|L-REGION+V-REGION| [len=347] (mis=4)
272-323 ==> 0-51 on |539|TRBJ1-4|J-REGION| [len=51] (mis=0)
323-375 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
cdr3 = CASRPTGTGTAETPYILER at 88, score = 8 + 4
umis assigned: [362, 612]
of which 2 are surviving nonsolos
reads assigned: 11896
start codons at 26, 83, 264, 278
confident = false
not full
VJ delta = -130
delta too large
not full
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.272 = GAACCTAGTCCGTTAA-1

using 118 reads

====================================================================================

graph has 98 edges initially, 6 edges after simplification

total ucounts = 8
nonsolo ucounts = 7[2^2, 3, 4, 18, 31, 57]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.274 = GAACCTAGTCCTCTTG-1

using 99 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[98]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.275 = GAACCTAGTCCTGCTT-1

using 132 reads

====================================================================================

graph has 98 edges initially, 4 edges after simplification

total ucounts = 14
nonsolo ucounts = 8[2^3, 3^3, 34, 77]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.281 = GAACCTAGTCTGATTG-1

using 73 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[73]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.282 = GAACCTAGTCTGCAAT-1

using 40 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[2^2, 3, 31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.283 = GAACCTAGTCTTCGTC-1

using 39 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[2, 18^2]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.284 = GAACCTAGTCTTGATG-1

using 44 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[44]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.288 = GAACCTAGTTACGTCA-1

using 58 reads

====================================================================================

graph has 88 edges initially, 4 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[4, 21, 31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.291 = GAACCTAGTTCCCGAG-1

using 16 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[2, 14]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.295 = GAACCTAGTTGATTCG-1

using 42 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[42]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.296 = GAACCTAGTTGATTGC-1

using 64 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[64]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.297 = GAACCTAGTTGCCTCT-1

using 37 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.298 = GAACCTAGTTGCTCCT-1

using 25 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.301 = GAACCTAGTTGTACAC-1

using 102 reads

====================================================================================

graph has 76 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 8[2^2, 3, 4^2, 6^2, 72]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.303 = GAACCTATCAAACAAG-1

using 7531 reads

====================================================================================

graph has 1488 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 7[2^3, 4, 8, 11, 7498]
surviving nonsolo ucounts = 1[7498]
ids = [6]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.304 = GAACCTATCAAACCAC-1

using 36 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.305 = GAACCTATCAACACGT-1

using 28 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.309 = GAACCTATCACATAGC-1

using 103 reads

====================================================================================

graph has 70 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[103]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.311 = GAACCTATCACCTTAT-1

using 84 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[8, 76]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.312 = GAACCTATCACTCTTA-1

using 192 reads

====================================================================================

graph has 134 edges initially, 6 edges after simplification

total ucounts = 12
nonsolo ucounts = 10[2, 3, 5^2, 9, 11, 15^2, 25, 100]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.313 = GAACCTATCACTTACT-1

using 111 reads

====================================================================================

graph has 28 edges initially, 4 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[45, 65]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.315 = GAACCTATCAGCTCTC-1

using 81 reads

====================================================================================

graph has 78 edges initially, 2 edges after simplification

total ucounts = 12
nonsolo ucounts = 6[2^3, 17, 22, 30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.317 = GAACCTATCAGTTAGC-1

using 131 reads

====================================================================================

graph has 125 edges initially, 2 edges after simplification

total ucounts = 12
nonsolo ucounts = 12[2, 4, 5^3, 6, 8, 10^2, 21, 27, 28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.318 = GAACCTATCAGTTTGG-1

using 207 reads

====================================================================================

graph has 528 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 5[2, 16, 23, 56, 110]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.319 = GAACCTATCATACGGT-1

using 93 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[93]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.320 = GAACCTATCATCACCC-1

using 31 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.322 = GAACCTATCATCTGCC-1

using 54 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[54]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.324 = GAACCTATCATGGTCA-1

using 113 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[112]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.325 = GAACCTATCATGTCCC-1

using 50 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[50]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.327 = GAACCTATCCGAACGC-1

using 55 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 6[2, 3, 4, 8^2, 28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.330 = GAACCTATCCGTCATC-1

using 265 reads

====================================================================================

graph has 380 edges initially, 22 edges after simplification

total ucounts = 19
nonsolo ucounts = 13[2, 8^3, 11^2, 20, 26, 29^3, 34, 44]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.331 = GAACCTATCCGTTGCT-1

using 67 reads

====================================================================================

graph has 120 edges initially, 2 edges after simplification

total ucounts = 13
nonsolo ucounts = 7[2^3, 3, 5, 10, 37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.335 = GAACCTATCCTTTACA-1

using 37 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.338 = GAACCTATCGCACTCT-1

using 64 reads

====================================================================================

graph has 64 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[2, 6, 55]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.339 = GAACCTATCGCCTGTT-1

using 144 reads

====================================================================================

graph has 197 edges initially, 6 edges after simplification

total ucounts = 8
nonsolo ucounts = 6[3^2, 22, 33, 40, 41]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.340 = GAACCTATCGGCATCG-1

using 138 reads

====================================================================================

graph has 160 edges initially, 4 edges after simplification

total ucounts = 17
nonsolo ucounts = 12[2^5, 5, 10^2, 11, 24, 27, 36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.342 = GAACCTATCGTACGGC-1

using 66 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[66]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.345 = GAACCTATCGTGGGAA-1

using 404 reads

====================================================================================

graph has 334 edges initially, 18 edges after simplification

total ucounts = 16
nonsolo ucounts = 15[5, 6, 10, 11^2, 16^2, 19, 20, 29^2, 36, 55, 68, 72]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.347 = GAACCTATCTACCTGC-1

using 23 reads

====================================================================================

graph has 6 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.349 = GAACCTATCTCCAGGG-1

using 110 reads

====================================================================================

graph has 112 edges initially, 4 edges after simplification

total ucounts = 11
nonsolo ucounts = 8[2^2, 5, 10, 11, 18, 25, 34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.352 = GAACCTATCTCTGCTG-1

using 50 reads

====================================================================================

graph has 58 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 5[5^2, 6, 14, 18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.357 = GAACCTATCTGCAGTA-1

using 17220 reads

====================================================================================

graph has 8855 edges initially, 289 edges after simplification

total ucounts = 838
nonsolo ucounts = 627[2^73, 3^51, 4^32, 5^38, 6^38, 7^30, 8^26, 9^35, 10^20, 11^22, 12^20, 13^11, 14^14, 15^14, 16^8, 17^18, 18^11, 19^19, 20^12, 21^12, 22^11, 23^2, 24^8, 25^10, 26^11, 27^10, 28^3, 29^5, 30^7, 31^3, 32^9, 33^3, 34^6, 35, 36^2, 37, 38, 39, 40^3, 41, 45^2, 48, 49^3, 51^2, 55^2, 56^2, 59, 62, 71, 73, 74, 81, 86, 87, 117, 130, 152, 870, 7453]
surviving nonsolo ucounts = 2[870, 7453]
ids = [227, 491]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.358 = GAACCTATCTGGTTCC-1

using 26917 reads

====================================================================================

graph has 16481 edges initially, 558 edges after simplification

total ucounts = 1757
nonsolo ucounts = 1372[2^196, 3^130, 4^93, 5^110, 6^78, 7^63, 8^51, 9^47, 10^41, 11^44, 12^42, 13^33, 14^31, 15^22, 16^23, 17^23, 18^23, 19^32, 20^18, 21^20, 22^23, 23^22, 24^17, 25^15, 26^20, 27^11, 28^17, 29^8, 30^13, 31^12, 32^5, 33^7, 34^2, 35^10, 36^6, 37^8, 38^3, 39^2, 40, 41^5, 42^4, 44^3, 45^2, 47, 48^3, 49^4, 51, 52^2, 53, 55^2, 56, 57^3, 58, 61, 62, 63, 70, 83, 87, 91, 96, 104, 110, 111, 115, 142, 564, 785, 2744, 5428]
surviving nonsolo ucounts = 4[564, 785, 2744, 5428]
ids = [1209, 828, 840, 1551]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.359 = GAACCTATCTGTGCAA-1

using 18 reads

====================================================================================

graph has 6 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.360 = GAACCTATCTTAACCT-1

using 181 reads

====================================================================================

graph has 186 edges initially, 4 edges after simplification

total ucounts = 12
nonsolo ucounts = 9[2, 3, 5, 7, 10, 12, 32, 36, 71]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.361 = GAACCTATCTTCAACT-1

using 111 reads

====================================================================================

graph has 211 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[111]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.362 = GAACCTATCTTCCTTC-1

using 484 reads

====================================================================================

graph has 110 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[484]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.363 = GAACCTATCTTGAGAC-1

using 369 reads

====================================================================================

graph has 228 edges initially, 14 edges after simplification

total ucounts = 17
nonsolo ucounts = 13[3, 9, 11, 12, 13, 16, 21, 22, 23, 40, 45, 67, 83]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.364 = GAACCTATCTTGCATT-1

using 83 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[83]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.368 = GAACGGAAGAAGGACA-1

using 34 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.369 = GAACGGAAGAAGGTTT-1

using 58 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[58]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.372 = GAACGGAAGACCCACC-1

using 209 reads

====================================================================================

graph has 124 edges initially, 8 edges after simplification

total ucounts = 10
nonsolo ucounts = 10[2, 3^2, 4, 5, 12, 27, 37, 48, 68]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.373 = GAACGGAAGAGAACAG-1

using 1323 reads

====================================================================================

graph has 406 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[1323]
surviving nonsolo ucounts = 1[1323]
ids = [0]

====================================================================================

UMI info for barcode GAACGGAAGAGAACAG-1 contig 1 = GACTGTGATT...
umi TTCAACATCC = 1323 reads: +391 validated

GOOD CONTIGS

TIG 1[bases=489]
0-56 ==> 0-56 on |529|TRAV9-2|5'UTR| [len=56] (mis=1)
56-394 ==> 0-338 on |530|TRAV9-2|L-REGION+V-REGION| [len=338] (mis=2)
395-447 ==> 11-63 on |425|TRAJ39|J-REGION| [len=63] (mis=0)
447-489 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 1 umis using 74 reads
cdr3 = CALREAGNMLTF at 380, score = 9 + 9
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 1304
start codons at 15, 56, 131, 219, 273, 404, 434
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.375 = GAACGGAAGAGGGCTT-1

using 61 reads

====================================================================================

graph has 56 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[2, 59]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.376 = GAACGGAAGAGTAAGG-1

using 51 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[51]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.385 = GAACGGAAGCCAGTAG-1

using 27 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.387 = GAACGGAAGCGTGAAC-1

using 596 reads

====================================================================================

graph has 394 edges initially, 6 edges after simplification

total ucounts = 25
nonsolo ucounts = 19[2, 3, 4^3, 5, 6, 8^2, 25, 26, 28, 33, 52, 54, 56, 75, 82, 115]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.388 = GAACGGAAGCTAGTGG-1

using 77 reads

====================================================================================

graph has 53 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 3[4, 14, 59]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.391 = GAACGGAAGGCCATAG-1

using 71 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 4[2, 3^2, 63]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.393 = GAACGGAAGGTAAACT-1

using 48 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[48]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.394 = GAACGGAAGGTACTCT-1

using 7442 reads

====================================================================================

graph has 5892 edges initially, 194 edges after simplification

total ucounts = 650
nonsolo ucounts = 494[2^62, 3^57, 4^39, 5^34, 6^32, 7^20, 8^22, 9^25, 10^24, 11^12, 12^8, 13^18, 14^12, 15^12, 16^6, 17^6, 18^5, 19^10, 20^7, 21^12, 22^10, 23^7, 24^3, 25^3, 26^5, 27^2, 28^2, 29, 30^2, 31, 32^3, 33, 34, 36, 39^3, 40, 44, 46, 51, 53, 54, 55, 58^2, 59, 60, 68^2, 78^2, 80, 84, 89, 91, 118, 133, 149, 169, 280, 306, 439]
surviving nonsolo ucounts = 5[21, 169, 280, 306, 439]
ids = [563, 217, 45, 52, 643]

====================================================================================

UMI info for barcode GAACGGAAGGTACTCT-1 contig 1 = AGCACAGACA...
umi AATTTTCTCT = 238 reads: -381X +3 -2XX +1 -5XX +2 -1XX +2 invalidated
umi ACATGTTCCT = 308 reads: +397 validated
umi CCCTACATCA = 171 reads: +397 validated
umi TCTTGTAGCC = 22 reads: -42 +280 -1X +5 -1 +1 -5 +2 -1 +8 -2 +3 -1 +1 -39 +5 invalidated
umi TTTGGTATCA = 393 reads: -381X +3 -2XX +1 -5XX +2 -1XX +2 invalidated

GOOD CONTIGS

TIG 1[bases=483]
0-34 ==> 275-309 on |630|TRBV5-1|5'UTR| [len=309] (mis=0)
34-377 ==> 0-343 on |631|TRBV5-1|L-REGION+V-REGION| [len=343] (mis=2)
389-431 ==> 6-48 on |537|TRBJ1-2|J-REGION| [len=48] (mis=0)
431-483 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 1 umis using 29 reads
cdr3 = CASSLPSGVDGYTF at 361, score = 9 + 9
umis assigned: [45, 52, 217, 563, 643]
of which 5 are surviving nonsolos
reads assigned: 1114
start codons at 34, 316, 320, 389
confident = true
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.396 = GAACGGAAGTACTTGC-1

using 69 reads

====================================================================================

graph has 62 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 5[2^2, 15, 19, 29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.401 = GAACGGACAAAGAATC-1

using 178 reads

====================================================================================

graph has 144 edges initially, 4 edges after simplification

total ucounts = 11
nonsolo ucounts = 9[2, 3, 4, 9, 15, 16, 34, 38, 55]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.402 = GAACGGACAAAGTCAA-1

using 47 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[2, 45]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.404 = GAACGGACAACACCTA-1

using 66 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[66]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.405 = GAACGGACAACTTGAC-1

using 31 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.407 = GAACGGACAAGCGATG-1

using 272 reads

====================================================================================

graph has 188 edges initially, 12 edges after simplification

total ucounts = 61
nonsolo ucounts = 42[2^12, 3^6, 4^7, 5^7, 7^2, 8^2, 9, 10, 11, 15, 20, 53]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.415 = GAACGGACACCGAATT-1

using 78 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[78]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.416 = GAACGGACACGCCAGT-1

using 100 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[100]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.418 = GAACGGACACTTAAGC-1

using 128 reads

====================================================================================

graph has 102 edges initially, 6 edges after simplification

total ucounts = 9
nonsolo ucounts = 9[2^2, 3, 6, 7, 8, 18, 31, 51]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.419 = GAACGGACAGACGCAA-1

using 58 reads

====================================================================================

graph has 52 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[2, 4, 50]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.426 = GAACGGACAGCTTCGG-1

using 80 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[80]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.433 = GAACGGACATCACAAC-1

using 90 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[90]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.435 = GAACGGACATCGGAAG-1

using 36 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 5[2^2, 3, 12, 16]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.436 = GAACGGACATCTGGTA-1

using 11606 reads

====================================================================================

graph has 10406 edges initially, 336 edges after simplification

total ucounts = 1189
nonsolo ucounts = 905[2^141, 3^88, 4^76, 5^67, 6^30, 7^55, 8^32, 9^32, 10^25, 11^37, 12^25, 13^28, 14^34, 15^25, 16^17, 17^22, 18^17, 19^22, 20^13, 21^10, 22^11, 23^8, 24^10, 25^12, 26^14, 27^9, 28^3, 29^3, 30^4, 31^2, 32^2, 33, 34, 36^4, 37, 38^3, 40, 41^2, 44, 47, 48, 50, 55^2, 59, 63, 64, 70, 79, 83, 95, 102, 103, 133, 447, 944]
surviving nonsolo ucounts = 3[25, 447, 944]
ids = [665, 1047, 667]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.439 = GAACGGAGTAAATGAC-1

using 283 reads

====================================================================================

graph has 159 edges initially, 13 edges after simplification

total ucounts = 11
nonsolo ucounts = 10[2^2, 4^2, 14, 17, 25, 55, 77, 82]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.444 = GAACGGAGTAGAAGGA-1

using 54421 reads

====================================================================================

graph has 20189 edges initially, 396 edges after simplification

total ucounts = 1089
nonsolo ucounts = 745[2^103, 3^76, 4^37, 5^37, 6^34, 7^32, 8^40, 9^27, 10^18, 11^27, 12^15, 13^10, 14^23, 15^9, 16^20, 17^10, 18^15, 19^12, 20^13, 21^14, 22^14, 23^14, 24^9, 25^13, 26^6, 27^6, 28^6, 29^8, 30^8, 31^11, 32^2, 33^7, 34^2, 35^4, 36^3, 37, 38^2, 39^4, 40, 41^2, 42, 43^2, 44^2, 46, 47^3, 48, 50, 53, 54, 55^2, 56, 71, 72, 81, 91, 96, 110, 127, 128, 262, 267, 586, 630, 667, 671, 900, 1012, 1225, 1246, 1390, 1441, 1448, 1490, 1670, 1821, 1946, 1989, 2056, 2156, 2191, 2310, 2315, 2591, 2923, 7308]
surviving nonsolo ucounts = 26[11, 128, 267, 630, 667, 671, 900, 1012, 1225, 1246, 1390, 1441, 1448, 1490, 1670, 1821, 1946, 1989, 2056, 2156, 2191, 2310, 2315, 2591, 2923, 7308]
ids = [533, 1032, 685, 529, 558, 659, 84, 294, 861, 632, ...]

====================================================================================

UMI info for barcode GAACGGAGTAGAAGGA-1 contig 1 = ACAGAAGTGG...
umi CCTGCAATGT = 946 reads: +397 validated
umi CGTTCTTTCT = 1453 reads: +397 validated
umi GTTACCACAG = 262 reads: +397 validated
umi TCACTTGAGG = 2198 reads: +397 validated

UMI info for barcode GAACGGAGTAGAAGGA-1 contig 2 = GTTCCCCTAT...
umi CCCGTTTCAG = 1387 reads: +397 validated
umi GAATATCACT = 1823 reads: +397 validated
umi GATCTTGCGG = 2005 reads: +397 validated
umi GCATATATTG = 660 reads: +382 -7 +8 non-validated
umi GGGTATCTGT = 1171 reads: +397 validated
umi TCACTTGTCG = 2042 reads: +397 validated
umi TTAGCCCACA = 1946 reads: +397 validated

UMI info for barcode GAACGGAGTAGAAGGA-1 contig 3 = AGAATGCTTA...
umi AGTGTCTCCT = 2145 reads: +400 validated
umi CCTGTTTCTT = 2598 reads: +400 validated
umi CTCATATCTC = 2707 reads: -367X +1 -1XX +1 -2XX +1 -5XX +1 -1XX +1 -1XX +1 -2XX +2 -7XX +1 -3XX +2 invalidated
umi CTCTTAGGGT = 1601 reads: -362 +1 -4XX +1 -1XX +1 -2XX +1 -5XX +1 -1XX +1 -1XX +1 -2XX +2 -7XX +1 -3XX +2 invalidated
umi GATAGTCCCG = 571 reads: -385X +2 -11XX +2 invalidated
umi GCATTACAGT = 5992 reads: -362 +1 -4XX +1 -1XX +1 -2XX +1 -5XX +1 -1XX +1 -1XX +1 -2XX +2 -7XX +1 -3XX +2 invalidated
umi GTTATTGGAA = 1503 reads: +400 validated
umi TATTCCGTTT = 1312 reads: -385X +2 -11XX +2 invalidated
umi TGACCCTCTG = 1 reads: -397X +2 -1X invalidated
umi TTCGCCGGCT = 2331 reads: +400 validated

GOOD CONTIGS

TIG 1[bases=508]
0-69 ==> 0-69 on |478|TRAV20|5'UTR| [len=69] (mis=0)
69-406 ==> 0-337 on |479|TRAV20|L-REGION+V-REGION| [len=337] (mis=4)
411-466 ==> 8-63 on |398|TRAJ13|J-REGION| [len=63] (mis=0)
466-508 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 4 umis using 222 reads
cdr3 = CAVPLEGGYQKVTF at 393, score = 8 + 8
umis assigned: [294, 371, 685, 802]
of which 4 are surviving nonsolos
reads assigned: 4787
start codons at 69, 78
confident = true

TIG 2[bases=506]
0-57 ==> 0-57 on |647|TRBV6-1|5'UTR| [len=57] (mis=0)
57-394 ==> 0-337 on |648|TRBV6-1|L-REGION+V-REGION| [len=344] (mis=0)
405-454 ==> 0-49 on |546|TRBJ2-3|J-REGION| [len=49] (mis=2)
454-506 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 6 umis using 613 reads
cdr3 = CASKRVEGTDTQYF at 384, score = 9 + 8
umis assigned: [252, 510, 541, 558, 632, 803, 976]
of which 7 are surviving nonsolos
reads assigned: 10828
start codons at 15, 57, 115, 168, 192, 207, 234, 298, 307
confident = true

TIG 3[bases=499]
0-47 ==> 0-47 on |692|TRBV9|5'UTR| [len=47] (mis=0)
47-390 ==> 0-343 on |693|TRBV9|L-REGION+V-REGION| [len=343] (mis=0)
405-447 ==> 8-50 on |543|TRBJ2-1|J-REGION| [len=50] (mis=0)
447-499 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 4 umis using 562 reads
cdr3 = CASSVGPLPQDEQFF at 374, score = 9 + 8
umis assigned: [71, 305, 413, 443, 529, 562, 687, 779, 915, 1013]
of which 9 are surviving nonsolos
reads assigned: 20459
start codons at 3, 47, 169, 255, 405
confident = true

REJECT CONTIGS

TIG 1[bases=493]
0-36 ==> 0-36 on |455|TRAV10|5'UTR| [len=36] (mis=0)
36-379 ==> 0-343 on |456|TRAV10|L-REGION+V-REGION| [len=343] (mis=1)
389-447 ==> 8-66 on |418|TRAJ32|J-REGION| [len=66] (mis=0)
451-493 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
cdr3 = CGERGRPGGATNKLIF at 368, score = 3 + 8
umis assigned: [822, 861, 1032]
of which 3 are surviving nonsolos
reads assigned: 2952
start codons at 36, 94, 164, 246, 298
confident = false
frameshifted full length transcript of length 493
VJ delta = 26
not full
not full
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.448 = GAACGGAGTATATCCG-1

using 51 reads

====================================================================================

graph has 66 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 3[4, 7, 40]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.449 = GAACGGAGTCAAAGAT-1

using 65 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[65]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.450 = GAACGGAGTCATACTG-1

using 27 reads

====================================================================================

graph has 31 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 1[25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.455 = GAACGGAGTCGTCTTC-1

using 27 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.462 = GAACGGAGTGTGGCTC-1

using 6411 reads

====================================================================================

graph has 1980 edges initially, 12 edges after simplification

total ucounts = 130
nonsolo ucounts = 80[2^22, 3^12, 4^4, 5^5, 6^6, 7^3, 8^3, 9^6, 10^3, 11, 12, 13, 15^2, 16^3, 17^3, 18, 23, 33, 141, 5695]
surviving nonsolo ucounts = 1[5695]
ids = [30]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.467 = GAACGGAGTTCAGGCC-1

using 2393 reads

====================================================================================

graph has 686 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 2[2, 2388]
surviving nonsolo ucounts = 1[2388]
ids = [2]

====================================================================================

UMI info for barcode GAACGGAGTTCAGGCC-1 contig 1 = ACCTGGAGCC...
umi CTCATTCATG = 2390 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=489]
0-43 ==> 0-43 on |610|TRBV27|5'UTR| [len=43] (mis=0)
43-387 ==> 0-344 on |611|TRBV27|L-REGION+V-REGION| [len=344] (mis=1)
389-437 ==> 0-48 on |536|TRBJ1-1|J-REGION| [len=48] (mis=3)
437-489 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 1 umis using 172 reads
cdr3 = CASSLTSYTEAFF at 370, score = 7 + 8
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 2353
start codons at 35, 43, 65, 178, 185, 193, 247, 251, 275
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.471 = GAACGGAGTTTGCATG-1

using 18 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.472 = GAACGGATCAAACAAG-1

using 40 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 3[7, 8, 25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.475 = GAACGGATCACGACTA-1

using 76 reads

====================================================================================

graph has 64 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[76]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.479 = GAACGGATCATGTGGT-1

using 40 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[2, 3, 34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.497 = GAACGGATCGGTTCGG-1

using 161 reads

====================================================================================

graph has 193 edges initially, 8 edges after simplification

total ucounts = 9
nonsolo ucounts = 7[5, 10, 15, 17, 19, 45, 48]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.501 = GAACGGATCTCATTCA-1

using 39 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[15, 24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.503 = GAACGGATCTGATTCT-1

using 37 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 3[3, 4, 30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.508 = GAAGCAGAGAAACCTA-1

using 20 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.511 = GAAGCAGAGAGTACCG-1

using 82 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 1[78]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.513 = GAAGCAGAGATGCCAG-1

using 40 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 3[4, 8, 25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.514 = GAAGCAGAGCACCGTC-1

using 61 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[61]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.520 = GAAGCAGAGCTCCCAG-1

using 49 reads

====================================================================================

graph has 60 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 4[2, 11, 16, 17]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.521 = GAAGCAGAGCTCCTTC-1

using 29 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[12, 16]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.527 = GAAGCAGAGGCCCGTT-1

using 38 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 3[8, 9, 21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.530 = GAAGCAGAGGTCATCT-1

using 59 reads

====================================================================================

graph has 52 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[8, 50]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.531 = GAAGCAGAGGTGTGGT-1

using 52 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 6[2^2, 6, 7, 11, 21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.535 = GAAGCAGAGTCTCGGC-1

using 96 reads

====================================================================================

graph has 82 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 5[2, 4, 5, 7, 75]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.536 = GAAGCAGAGTCTTGCA-1

using 97 reads

====================================================================================

graph has 52 edges initially, 6 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[3, 23, 24, 45]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.537 = GAAGCAGAGTGAAGTT-1

using 2223 reads

====================================================================================

graph has 744 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 5[4, 8, 16, 22, 2169]
surviving nonsolo ucounts = 2[22, 2169]
ids = [6, 2]

====================================================================================

UMI info for barcode GAAGCAGAGTGAAGTT-1 contig 1 = AGACCAGAAT...
umi ATGGGAACAT = 2154 reads: +394 validated
umi GTGGACCGGT = 23 reads: +50 -5 +129 -36 +73 -1 +2 -1 +2 -1 +11 -1 +4 -1 +1 -1 +9 -1 +2 -1 +62 non-validated

GOOD CONTIGS

TIG 1[bases=493]
0-47 ==> 0-47 on |616|TRBV3-1|5'UTR| [len=47] (mis=0)
47-391 ==> 0-344 on |617|TRBV3-1|L-REGION+V-REGION| [len=344] (mis=0)
401-441 ==> 8-48 on |537|TRBJ1-2|J-REGION| [len=48] (mis=0)
441-493 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 1 umis using 149 reads
cdr3 = CASSQEGAQGYTF at 374, score = 9 + 9
umis assigned: [2, 6]
of which 2 are surviving nonsolos
reads assigned: 2141
start codons at 47, 146, 169, 189, 197, 239, 273
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.541 = GAAGCAGCAAACGCGA-1

using 12028 reads

====================================================================================

graph has 6884 edges initially, 104 edges after simplification

total ucounts = 586
nonsolo ucounts = 409[2^83, 3^55, 4^40, 5^32, 6^21, 7^17, 8^20, 9^23, 10^13, 11^12, 12^5, 13^7, 14^10, 15^5, 16^3, 17^5, 18^6, 19^6, 20^7, 21^2, 22^3, 23^3, 24, 25, 26, 28, 29^2, 30, 33, 34, 35, 36^2, 40, 49, 51, 55, 56, 66, 67, 68, 75, 80, 84, 89, 109, 827, 988, 1072, 1414, 1682, 2051]
surviving nonsolo ucounts = 6[827, 988, 1072, 1414, 1682, 2051]
ids = [541, 103, 135, 289, 225, 301]

====================================================================================

UMI info for barcode GAAGCAGCAAACGCGA-1 contig 1 = ACAGAAGTGG...
umi AGGTTCCTGC = 85 reads: -368X +3 -1XX +2 -1XX +1 -2XX +5 -1XX +2 -1XX +1 -2XX +2 -2XX invalidated
umi CCCCTCCACT = 1694 reads: +394 validated
umi CTGATTTTTA = 1366 reads: +394 validated
umi CTTACATGTC = 2055 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=505]
0-69 ==> 0-69 on |478|TRAV20|5'UTR| [len=69] (mis=0)
69-406 ==> 0-337 on |479|TRAV20|L-REGION+V-REGION| [len=337] (mis=0)
404-463 ==> 0-59 on |412|TRAJ27|J-REGION| [len=59] (mis=3)
463-505 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 3 umis using 251 reads
cdr3 = CAVQATNAGKSTF at 393, score = 8 + 8
umis assigned: [103, 225, 289, 301]
of which 4 are surviving nonsolos
reads assigned: 5100
start codons at 69, 78, 412, 436
confident = true
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.548 = GAAGCAGCACAGCCCA-1

using 36 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 2[4, 28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.549 = GAAGCAGCACAGTCGC-1

using 357 reads

====================================================================================

graph has 262 edges initially, 10 edges after simplification

total ucounts = 20
nonsolo ucounts = 12[4, 6, 10, 13^3, 14, 22, 41, 53, 78, 82]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.552 = GAAGCAGCACCGAATT-1

using 140 reads

====================================================================================

graph has 86 edges initially, 4 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[62, 78]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.555 = GAAGCAGCACGTTGGC-1

using 225 reads

====================================================================================

graph has 172 edges initially, 8 edges after simplification

total ucounts = 9
nonsolo ucounts = 8[3, 4, 11, 14, 40, 44, 49, 59]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.556 = GAAGCAGCACTCGACG-1

using 120 reads

====================================================================================

graph has 60 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[120]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.563 = GAAGCAGCAGGAATGC-1

using 45 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[45]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.564 = GAAGCAGCAGGGATTG-1

using 164 reads

====================================================================================

graph has 116 edges initially, 6 edges after simplification

total ucounts = 8
nonsolo ucounts = 6[4^2, 6, 16, 41, 91]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.575 = GAAGCAGCATCTCCCA-1

using 23 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.576 = GAAGCAGCATTATCTC-1

using 144 reads

====================================================================================

graph has 214 edges initially, 4 edges after simplification

total ucounts = 6
nonsolo ucounts = 6[4, 5, 10, 12, 27, 86]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.579 = GAAGCAGGTAAGGATT-1

using 32 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.582 = GAAGCAGGTAATCACC-1

using 4453 reads

====================================================================================

graph has 776 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 1[4449]
surviving nonsolo ucounts = 1[4449]
ids = [2]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.583 = GAAGCAGGTACGAAAT-1

using 30 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.588 = GAAGCAGGTCGTCTTC-1

using 228 reads

====================================================================================

graph has 182 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[228]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.591 = GAAGCAGGTCTAGTCA-1

using 44 reads

====================================================================================

graph has 48 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[7, 36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.594 = GAAGCAGGTGACCAAG-1

using 2210 reads

====================================================================================

graph has 708 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 3[2, 4, 2199]
surviving nonsolo ucounts = 1[2199]
ids = [6]

====================================================================================

UMI info for barcode GAAGCAGGTGACCAAG-1 contig 1 = ACCTGGAGCC...
umi TCCATAACCT = 2202 reads: +403 validated

GOOD CONTIGS

TIG 1[bases=498]
0-43 ==> 0-43 on |610|TRBV27|5'UTR| [len=43] (mis=0)
43-387 ==> 0-344 on |611|TRBV27|L-REGION+V-REGION| [len=344] (mis=5)
404-446 ==> 8-50 on |543|TRBJ2-1|J-REGION| [len=50] (mis=0)
446-498 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 1 umis using 94 reads
cdr3 = CASSPTSGQLGHEQFF at 370, score = 7 + 8
umis assigned: [6]
of which 1 are surviving nonsolos
reads assigned: 2170
start codons at 35, 43, 65, 178, 185, 193, 247, 251, 275, 404
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.595 = GAAGCAGGTGAGGGAG-1

using 35 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.596 = GAAGCAGGTGCGGTAA-1

using 1212 reads

====================================================================================

graph has 468 edges initially, 4 edges after simplification

total ucounts = 7
nonsolo ucounts = 5[2, 5, 6, 16, 1181]
surviving nonsolo ucounts = 1[1181]
ids = [2]

====================================================================================

UMI info for barcode GAAGCAGGTGCGGTAA-1 contig 1 = CCTGAGCACA...
umi ATAACTTGCA = 1182 reads: +409 validated

GOOD CONTIGS

TIG 1[bases=499]
0-38 ==> 271-309 on |630|TRBV5-1|5'UTR| [len=309] (mis=0)
38-381 ==> 0-343 on |631|TRBV5-1|L-REGION+V-REGION| [len=343] (mis=0)
397-447 ==> 0-50 on |543|TRBJ2-1|J-REGION| [len=50] (mis=5)
447-499 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 1 umis using 70 reads
cdr3 = CASSLGRGRGRFGNEQFF at 365, score = 9 + 8
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 1163
start codons at 38, 320, 324, 405
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.597 = GAAGCAGGTGCTTCTC-1

using 25 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.599 = GAAGCAGGTGGTCTCG-1

using 13176 reads

====================================================================================

graph has 5531 edges initially, 104 edges after simplification

total ucounts = 540
nonsolo ucounts = 362[2^52, 3^47, 4^25, 5^18, 6^13, 7^23, 8^22, 9^11, 10^17, 11^12, 12^10, 13^12, 14^15, 15^10, 16^7, 17^5, 18^10, 19^7, 20^6, 21^3, 23^2, 24^4, 25^2, 26^3, 27^3, 28^4, 29^2, 30, 31^3, 32, 36, 39^2, 41, 42, 43, 55, 72, 73, 106, 113, 9083]
surviving nonsolo ucounts = 1[9083]
ids = [304]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.600 = GAAGCAGGTTACGCGC-1

using 22 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.608 = GAAGCAGGTTGTGGAG-1

using 395 reads

====================================================================================

graph has 290 edges initially, 8 edges after simplification

total ucounts = 66
nonsolo ucounts = 34[2^4, 3^4, 4^3, 5^4, 6^5, 7, 8, 9^2, 10^2, 11^2, 14^2, 15, 18, 41, 104]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.609 = GAAGCAGTCAACACCA-1

using 78 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[78]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.612 = GAAGCAGTCACATAGC-1

using 84 reads

====================================================================================

graph has 60 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[7, 12, 64]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.614 = GAAGCAGTCACCAGGC-1

using 80 reads

====================================================================================

graph has 114 edges initially, 4 edges after simplification

total ucounts = 3
nonsolo ucounts = 3[4, 18, 58]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.616 = GAAGCAGTCACTATTC-1

using 152 reads

====================================================================================

graph has 138 edges initially, 6 edges after simplification

total ucounts = 12
nonsolo ucounts = 10[2, 3^2, 4^2, 8, 10, 12, 31, 73]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.617 = GAAGCAGTCACTGGGC-1

using 27 reads

====================================================================================

graph has 6 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.620 = GAAGCAGTCATAGCAC-1

using 12 reads

====================================================================================

graph has 6 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[12]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.622 = GAAGCAGTCATGTGGT-1

using 33 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.623 = GAAGCAGTCCAAACTG-1

using 79 reads

====================================================================================

graph has 64 edges initially, 4 edges after simplification

total ucounts = 5
nonsolo ucounts = 2[25, 51]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.624 = GAAGCAGTCCAAATGC-1

using 75 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[75]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.625 = GAAGCAGTCCACGAAT-1

using 60 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[60]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.626 = GAAGCAGTCCATTCTA-1

using 85 reads

====================================================================================

graph has 67 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[85]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.628 = GAAGCAGTCCGCGGTA-1

using 34 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 1[30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.629 = GAAGCAGTCCTATGTT-1

using 49 reads

====================================================================================

graph has 48 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[48]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.631 = GAAGCAGTCCTGTACC-1

using 30 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.636 = GAAGCAGTCGCTTAGA-1

using 599 reads

====================================================================================

graph has 480 edges initially, 6 edges after simplification

total ucounts = 40
nonsolo ucounts = 28[2^6, 3^3, 6, 9^3, 11, 12^2, 13, 17^2, 19^2, 23, 43, 44, 47, 66, 80, 110]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.640 = GAAGCAGTCGTTGCCT-1

using 77 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[76]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.643 = GAAGCAGTCTATGTGG-1

using 294 reads

====================================================================================

graph has 253 edges initially, 10 edges after simplification

total ucounts = 17
nonsolo ucounts = 14[2, 3, 4, 8, 10, 11, 12, 13, 27, 28, 34, 40, 47, 52]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.647 = GAAGCAGTCTGTGCAA-1

using 16 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[16]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.653 = GAATAAGAGATATACG-1

using 35 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.656 = GAATAAGAGCAAATCA-1

using 272 reads

====================================================================================

graph has 202 edges initially, 8 edges after simplification

total ucounts = 13
nonsolo ucounts = 13[2^2, 6^2, 7, 12, 13, 15, 25, 26, 45, 46, 67]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.657 = GAATAAGAGCACCGTC-1

using 61 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[61]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.662 = GAATAAGAGCGTAGTG-1

using 44 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[44]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.664 = GAATAAGAGCTACCGC-1

using 32740 reads

====================================================================================

graph has 12615 edges initially, 126 edges after simplification

total ucounts = 428
nonsolo ucounts = 302[2^39, 3^28, 4^24, 5^14, 6^13, 7^10, 8^18, 9^12, 10^5, 11^6, 12^5, 13^4, 14^8, 15^11, 16^4, 17^6, 18^6, 19^6, 20^8, 21^12, 22^2, 23^2, 24^5, 25^3, 26^2, 27^5, 30, 31, 32^2, 33^3, 34, 36^2, 38^2, 39^2, 41, 45, 49, 59, 68, 76, 85, 96, 100, 108, 230, 386, 795, 812, 827, 901, 936, 970, 1033, 1102, 1150, 1226, 1275, 1380, 1769, 2050, 2085, 2622, 3340, 4009]
surviving nonsolo ucounts = 21[85, 230, 386, 795, 812, 827, 901, 936, 970, 1033, 1102, 1150, 1226, 1275, 1380, 1769, 2050, 2085, 2622, 3340, 4009]
ids = [316, 349, 417, 215, 256, 393, 416, 39, 326, 254, ...]

====================================================================================

UMI info for barcode GAATAAGAGCTACCGC-1 contig 1 = AGGAGCAGTG...
umi AAAAATTCGC = 2606 reads: +406 validated
umi ACACACCTCT = 937 reads: +406 validated
umi AGTGCATCTA = 1227 reads: +406 validated
umi ATATATCGGG = 2104 reads: +406 validated
umi ATGCAACGTA = 1103 reads: +406 validated
umi CGTCAGCGCG = 1286 reads: +406 validated
umi GAGTATGCGG = 2059 reads: +406 validated
umi GCTTCTCTGT = 1031 reads: +406 validated
umi GGACATGCAT = 813 reads: +406 validated
umi GTCCGACGGA = 1153 reads: +406 validated
umi TCACTCAATC = 970 reads: +406 validated
umi TCGGGCTCGG = 1384 reads: +406 validated
umi TCTGACTTCA = 228 reads: +406 validated
umi TGATCGCGAT = 3353 reads: +406 validated
umi TTCCGTCTCC = 830 reads: +406 validated
umi TTTATTGTGT = 902 reads: +406 validated
umi TTTCACATCT = 389 reads: +406 validated

UMI info for barcode GAATAAGAGCTACCGC-1 contig 2 = CTGGGCTCAT...
umi CAAACGACCC = 4182 reads: +6 -1XX +1 -2XX +1 -1XX +1 -1XX +1 -7XX +1 -1XX +1 -8XX +1 -6XX +348 invalidated
umi CATAGCGTCC = 1786 reads: +388 validated
umi GCCATCGCAG = 76 reads: -371X +1 -1XX +1 -1XX +1 -3XX +3 -5XX +1 invalidated

UMI info for barcode GAATAAGAGCTACCGC-1 contig 3 = GGGGGCATAG...
umi CTTTTGTGGT = 761 reads: +449 -1X +1 invalidated
umi TATTCCCACC = 80 reads: -13 +438 non-validated

GOOD CONTIGS

TIG 1[bases=569]
0-111 ==> 0-111 on |626|TRBV4-2|5'UTR| [len=111] (mis=0)
111-455 ==> 0-344 on |627|TRBV4-2|L-REGION+V-REGION| [len=344] (mis=1)
469-517 ==> 0-48 on |548|TRBJ2-5|J-REGION| [len=48] (mis=2)
517-569 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 16 umis using 1167 reads
cdr3 = CASSQDASGRGQETQYF at 438, score = 10 + 7
umis assigned: [3, 39, 80, 89, 104, 189, 227, 254, 256, 278] and 7 others
of which 17 are surviving nonsolos
reads assigned: 21896
start codons at 111, 165, 204, 210, 233, 261, 303, 368
confident = true

TIG 2[bases=497]
0-67 ==> 0-67 on |509|TRAV4|5'UTR| [len=67] (mis=0)
67-395 ==> 0-328 on |510|TRAV4|L-REGION+V-REGION| [len=328] (mis=4)
392-455 ==> 0-63 on |398|TRAJ13|J-REGION| [len=63] (mis=6)
455-497 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 2 umis using 343 reads
cdr3 = CLVVVASGGYQKVTF at 379, score = 8 + 8
umis assigned: [120, 143, 243]
of which 2 are surviving nonsolos
reads assigned: 5748
start codons at 67, 145, 155, 206
confident = true

TIG 3[bases=670]
131-528 ==> 0-397 on |516|TRAV6|L-REGION+V-REGION| [len=397] (mis=6)
530-582 ==> 8-60 on |396|TRAJ11|J-REGION| [len=60] (mis=0)
junction support: 2 umis using 36 reads
cdr3 = CALLRGYSTLTF at 515, score = 8 + 8
umis assigned: [215, 316]
of which 2 are surviving nonsolos
reads assigned: 823
start codons at 25, 131, 188, 405, 563
confident = true
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.666 = GAATAAGAGCTATGCT-1

using 45 reads

====================================================================================

graph has 72 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 8[2^2, 3^2, 7, 8^2, 9]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.670 = GAATAAGAGCTGCAAG-1

using 67880 reads

====================================================================================

graph has 20683 edges initially, 260 edges after simplification

total ucounts = 672
nonsolo ucounts = 467[2^63, 3^29, 4^36, 5^27, 6^21, 7^18, 8^14, 9^18, 10^11, 11^14, 12^12, 13^7, 14^8, 15^8, 16^11, 17^17, 18^12, 19^11, 20^11, 21^8, 22^7, 23^11, 24^10, 25^4, 26^7, 27^6, 28^2, 29^2, 30^4, 31^2, 32^4, 33, 34, 36^2, 37^2, 38, 40, 42^2, 44, 48, 49, 50, 52, 57, 60, 67, 68, 69, 76, 79, 86, 90, 96, 107, 109, 114, 150, 155, 169, 192, 244, 511, 933, 1224, 1303, 1332, 1351, 1649, 1724, 1787, 1858, 2405, 2412, 2536, 3275, 3638, 5046, 8563, 8683, 10297]
surviving nonsolo ucounts = 21[169, 192, 511, 933, 1224, 1303, 1332, 1351, 1649, 1724, 1787, 1858, 2405, 2412, 2536, 3275, 3638, 5046, 8563, 8683, 10297]
ids = [544, 170, 122, 169, 278, 561, 38, 407, 592, 320, ...]

====================================================================================

UMI info for barcode GAATAAGAGCTGCAAG-1 contig 1 = AAGCACTCTT...
umi ACGATCGGCC = 7518 reads: -352X +1 -1XX +6 -2XX +3 -1XX +2 -1XX +3 -1XX +1 -4XX +3 -3XX +2 -1XX +1 invalidated
umi AGTAGGCCGC = 471 reads: -362X +6 -1XX +2 -2XX +1 -2XX +1 -2XX +2 -3XX +2 -2XX invalidated
umi ATTCAGCTAC = 942 reads: +388 validated
umi CCGTCTACTC = 8600 reads: -354X +5 -3XX +6 -1XX +2 -2XX +1 -2XX +1 -2XX +2 -3XX +2 -2XX invalidated
umi GAACTCTCAT = 1727 reads: +388 validated
umi GGGAACATCA = 1351 reads: +388 validated
umi TATTCAAGAG = 7695 reads: -352X +1 -1XX +6 -2XX +3 -1XX +2 -1XX +3 -1XX +1 -4XX +3 -3XX +2 -1XX +1 invalidated
umi TCACCAGGCA = 4511 reads: -352X +1 -1XX +6 -2XX +3 -1XX +2 -1XX +3 -1XX +1 -4XX +3 -3XX +2 -1XX +1 invalidated
umi TGCAAATGGA = 1642 reads: +388 validated

UMI info for barcode GAATAAGAGCTGCAAG-1 contig 2 = AGAGGCCCCA...
umi AACTCGCTCT = 2541 reads: +409 validated
umi AATTTGTCTC = 1364 reads: +49 -1XX +359 invalidated
umi CCCCATCACG = 1799 reads: +409 validated
umi CGGTCTCGCC = 1229 reads: +409 validated
umi TAACTTCGAC = 2415 reads: +409 validated
umi TCGGCGAAGG = 892 reads: +38 -1XX +1 -6XX +1 -1XX +361 invalidated
umi TTCTTCCTCC = 1863 reads: +409 validated
umi TTGTCGGGTC = 2427 reads: +409 validated

GOOD CONTIGS

TIG 1[bases=527]
0-97 ==> 0-97 on |492|TRAV27|5'UTR| [len=97] (mis=0)
97-425 ==> 0-328 on |493|TRAV27|L-REGION+V-REGION| [len=328] (mis=3)
422-485 ==> 0-63 on |409|TRAJ24|J-REGION| [len=63] (mis=4)
485-527 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 4 umis using 360 reads
cdr3 = CAGVTDSWGKFQF at 415, score = 10 + 6
umis assigned: [73, 122, 169, 247, 320, 407, 512, 528, 592]
of which 9 are surviving nonsolos
reads assigned: 34273
start codons at 70, 79, 97, 141, 249, 350
confident = true

TIG 2[bases=490]
0-29 ==> 82-111 on |628|TRBV4-3|5'UTR| [len=111] (mis=0)
29-373 ==> 0-344 on |629|TRBV4-3|L-REGION+V-REGION| [len=344] (mis=0)
383-395 ==> 0-12 on |535|TRBD1|D-REGION| [len=12] (mis=1)
391-438 ==> 0-47 on |550|TRBJ2-7|J-REGION| [len=47] (mis=4)
438-490 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 8 umis using 1035 reads
cdr3 = CASSQDTQPGTGGYEQYF at 356, score = 10 + 8
umis assigned: [10, 38, 229, 278, 468, 561, 649, 658]
of which 8 are surviving nonsolos
reads assigned: 14217
start codons at 29, 83, 122, 128, 151, 179, 221, 286
confident = true

REJECT CONTIGS

TIG 1[bases=315]
5-62 ==> 47-104 on segment before TRBJ2-3 exon 1 [len=104] (mis=0)
62-111 ==> 0-49 on |546|TRBJ2-3|J-REGION| [len=49] (mis=0)
111-213 ==> 0-102 on segment before TRBJ2-4 exon 1 [len=102] (mis=0)
263-315 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
umis assigned: [13, 61, 170, 232, 544]
of which 4 are surviving nonsolos
reads assigned: 7061
start codons at 
confident = false
did not find CDR3
now this is a cell
paired!

GCCCAGCCTGGTGATACAGGCCTCTACCTCTGTGCAGGGGTTACTGACAGCTGGGGGAAATTCCAGTTTGGAGCAGGGACCCAGGTTGTGGTCACCCCAG <==> GACTCGGCCCTGTATCTCTGCGCCAGCAGCCAAGATACTCAACCCGGGACAGGGGGATACGAGCAGTACTTCGGGCCGGGCACCAGGCTCACGGTCACAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.671 = GAATAAGAGGAATTAC-1

using 204 reads

====================================================================================

graph has 126 edges initially, 4 edges after simplification

total ucounts = 9
nonsolo ucounts = 9[2^2, 4^2, 6^2, 8, 67, 105]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.672 = GAATAAGAGGACTGGT-1

using 93 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[93]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.678 = GAATAAGAGGTAGCCA-1

using 58 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[58]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.683 = GAATAAGAGTGTTGAA-1

using 22 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.687 = GAATAAGCAACACCTA-1

using 51 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[51]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.689 = GAATAAGCAACTGGCC-1

using 40 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[40]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.691 = GAATAAGCAAGACGTG-1

using 85 reads

====================================================================================

graph has 62 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[85]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.694 = GAATAAGCACACTGCG-1

using 33175 reads

====================================================================================

graph has 19303 edges initially, 680 edges after simplification

total ucounts = 1240
nonsolo ucounts = 1055[2^109, 3^60, 4^57, 5^46, 6^35, 7^33, 8^32, 9^29, 10^22, 11^25, 12^26, 13^21, 14^19, 15^21, 16^16, 17^8, 18^10, 19^20, 20^10, 21^10, 22^7, 23^8, 24^9, 25^2, 26^7, 27^8, 28^9, 29^8, 30^6, 31^6, 32^10, 33^6, 34^7, 35^10, 36^7, 37^6, 38^7, 39^7, 40^5, 41^7, 42^9, 43^3, 44^2, 45^6, 46^8, 47^10, 48^2, 49^3, 50^4, 51^3, 52^4, 53^5, 54^5, 55^3, 56^8, 57^7, 58^6, 59^5, 60^4, 61^3, 62^3, 63^3, 64^5, 65^7, 66, 67^6, 68^4, 69^3, 70^6, 71^4, 72^3, 73^4, 74^4, 75^5, 76^3, 77^2, 78^4, 79^2, 80^5, 81^5, 82^4, 83^6, 84^2, 85^3, 86^3, 87^2, 88^4, 89, 90^4, 91^6, 92^4, 93, 94^2, 95^5, 97^6, 98^7, 99^3, 100^3, 101^3, 102^5, 103^2, 104, 106^2, 107^2, 108^2, 109^4, 110, 111^3, 112, 113^4, 115, 116, 117^2, 118, 119^3, 120, 121^2, 124^3, 125, 128, 129^2, 130^2, 131, 132^3, 144, 145, 146, 151, 155]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.695 = GAATAAGCACATTAGC-1

using 184 reads

====================================================================================

graph has 268 edges initially, 4 edges after simplification

total ucounts = 8
nonsolo ucounts = 6[3, 8, 10, 11, 14, 136]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.696 = GAATAAGCACATTCGA-1

using 41 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[41]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.701 = GAATAAGCACTAGTAC-1

using 35 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.702 = GAATAAGCACTCGACG-1

using 91 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[91]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.703 = GAATAAGCACTTCGAA-1

using 23 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.705 = GAATAAGCAGCATGAG-1

using 47 reads

====================================================================================

graph has 62 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 5[3^2, 5, 12, 21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.712 = GAATAAGCATCAGTCA-1

using 34 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.716 = GAATAAGGTAAATGAC-1

using 711 reads

====================================================================================

graph has 250 edges initially, 6 edges after simplification

total ucounts = 9
nonsolo ucounts = 8[2, 3, 4^2, 6, 23, 40, 628]
surviving nonsolo ucounts = 1[628]
ids = [2]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.721 = GAATAAGGTAGCTGCC-1

using 3980 reads

====================================================================================

graph has 995 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 3[5, 6, 3964]
surviving nonsolo ucounts = 1[3964]
ids = [7]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.736 = GAATAAGGTGTTTGGT-1

using 81 reads

====================================================================================

graph has 92 edges initially, 4 edges after simplification

total ucounts = 7
nonsolo ucounts = 5[7, 14, 19^2, 20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.739 = GAATAAGGTTGCGCAC-1

using 29 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.740 = GAATAAGGTTGTGGAG-1

using 458 reads

====================================================================================

graph has 344 edges initially, 14 edges after simplification

total ucounts = 24
nonsolo ucounts = 22[2^2, 4^2, 5, 7, 8, 10^2, 13, 15^2, 18, 20, 21, 22, 28, 39, 40^2, 50, 83]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.744 = GAATAAGTCACATGCA-1

using 1882 reads

====================================================================================

graph has 818 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 1[1879]
surviving nonsolo ucounts = 1[1879]
ids = [1]

====================================================================================

UMI info for barcode GAATAAGTCACATGCA-1 contig 1 = GTCTACCTTC...
umi ATAATGAGCA = 1887 reads: +391 validated

GOOD CONTIGS

TIG 1[bases=505]
0-72 ==> 12-84 on |453|TRAV1-2|5'UTR| [len=84] (mis=0)
72-390 ==> 0-318 on |454|TRAV1-2|L-REGION+V-REGION| [len=318] (mis=1)
408-463 ==> 11-66 on |418|TRAJ32|J-REGION| [len=66] (mis=0)
463-505 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 1 umis using 94 reads
cdr3 = CAVGSVFGGATNKLIF at 384, score = 7 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 1838
start codons at 21, 39, 72, 94, 102, 108, 150, 235, 268, 277, 360
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.749 = GAATAAGTCAGTACGT-1

using 142 reads

====================================================================================

graph has 128 edges initially, 4 edges after simplification

total ucounts = 6
nonsolo ucounts = 5[3, 4, 6, 38, 90]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.754 = GAATAAGTCCCTGACT-1

using 50 reads

====================================================================================

graph has 32 edges initially, 6 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[8, 42]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.755 = GAATAAGTCCGAGCCA-1

using 611 reads

====================================================================================

graph has 298 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[2, 3, 68, 536]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.756 = GAATAAGTCCGCATCT-1

using 39 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[2, 15, 21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.757 = GAATAAGTCCGCGGTA-1

using 133 reads

====================================================================================

graph has 54 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 3[15, 18, 96]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.759 = GAATAAGTCCGTAGTA-1

using 12 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[12]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.760 = GAATAAGTCCTAAGTG-1

using 58 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[58]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.762 = GAATAAGTCCTTGGTC-1

using 5996 reads

====================================================================================

graph has 5499 edges initially, 230 edges after simplification

total ucounts = 521
nonsolo ucounts = 415[2^45, 3^29, 4^23, 5^17, 6^20, 7^21, 8^12, 9^18, 10^9, 11^16, 12^11, 13^15, 14^15, 15^6, 16^19, 17^8, 18^12, 19^8, 20^7, 21^9, 22^10, 23^6, 24^11, 25^5, 26^6, 27^5, 28^7, 29^6, 30^3, 31^2, 32^6, 33, 34^7, 35^4, 36^3, 38, 39, 40^2, 41, 42, 43, 45, 50, 57, 63, 106^2]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.764 = GAATAAGTCGATAGAA-1

using 53 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[53]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.767 = GAATAAGTCGCAGGCT-1

using 96 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[8, 88]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.769 = GAATAAGTCGGACAAG-1

using 35 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.771 = GAATAAGTCGGCATCG-1

using 104 reads

====================================================================================

graph has 54 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[104]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.772 = GAATAAGTCGGCGCAT-1

using 107 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[107]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.774 = GAATAAGTCTACTATC-1

using 141 reads

====================================================================================

graph has 94 edges initially, 4 edges after simplification

total ucounts = 12
nonsolo ucounts = 8[3^2, 4, 6, 7^2, 16, 91]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.777 = GAATAAGTCTGGTATG-1

using 65 reads

====================================================================================

graph has 68 edges initially, 4 edges after simplification

total ucounts = 9
nonsolo ucounts = 5[2, 12, 14, 15, 18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.781 = GAATGAAAGAACTCGG-1

using 96 reads

====================================================================================

graph has 64 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[95]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.782 = GAATGAAAGAATAGGG-1

using 76 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 3[3, 5, 63]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.785 = GAATGAAAGACTACAA-1

using 78 reads

====================================================================================

graph has 48 edges initially, 6 edges after simplification

total ucounts = 11
nonsolo ucounts = 7[2^4, 3, 24, 39]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.786 = GAATGAAAGAGATGAG-1

using 180 reads

====================================================================================

graph has 164 edges initially, 4 edges after simplification

total ucounts = 13
nonsolo ucounts = 11[3^2, 6^3, 8, 10, 12, 16, 51, 57]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.787 = GAATGAAAGATAGGAG-1

using 56 reads

====================================================================================

graph has 64 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 4[2, 5, 17, 28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.791 = GAATGAAAGCCTATGT-1

using 88 reads

====================================================================================

graph has 96 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 7[5, 6, 8, 12, 13, 21, 22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.796 = GAATGAAAGCTAACAA-1

using 18 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.798 = GAATGAAAGCTAGTGG-1

using 45 reads

====================================================================================

graph has 76 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[3, 4, 37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.799 = GAATGAAAGCTCCTCT-1

using 43 reads

====================================================================================

graph has 68 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[8^2, 25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.802 = GAATGAAAGGACACCA-1

using 119 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[119]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.808 = GAATGAAAGGTTACCT-1

using 35 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.809 = GAATGAAAGTAATCCC-1

using 543 reads

====================================================================================

graph has 444 edges initially, 16 edges after simplification

total ucounts = 53
nonsolo ucounts = 38[2^3, 3^4, 4^4, 5, 6^3, 8^2, 10^3, 11, 12, 13, 14, 15^2, 17^3, 19, 20, 26, 30, 32^2, 35, 50^2]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.813 = GAATGAAAGTGCCAGA-1

using 115 reads

====================================================================================

graph has 142 edges initially, 4 edges after simplification

total ucounts = 14
nonsolo ucounts = 8[2^2, 3, 5, 10, 12, 28, 47]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.814 = GAATGAAAGTGGACGT-1

using 100 reads

====================================================================================

graph has 112 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 8[2, 3, 4, 6, 12, 13, 26, 31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.815 = GAATGAAAGTTAAGTG-1

using 110 reads

====================================================================================

graph has 96 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 7[3^3, 4, 8, 34, 51]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.817 = GAATGAACAAGAGGCT-1

using 9832 reads

====================================================================================

graph has 8809 edges initially, 363 edges after simplification

total ucounts = 985
nonsolo ucounts = 728[2^97, 3^76, 4^46, 5^37, 6^38, 7^35, 8^30, 9^25, 10^20, 11^29, 12^28, 13^23, 14^24, 15^27, 16^15, 17^16, 18^9, 19^7, 20^11, 21^12, 22^8, 23^8, 24^9, 25^6, 26^7, 27^6, 28^3, 29^7, 30^9, 31^5, 32^3, 33^3, 34^3, 35^5, 36^3, 37^4, 38^3, 39^5, 40^5, 43, 44, 46, 48, 50^2, 52, 54, 55, 64, 66, 67, 68, 73, 82, 83, 94, 104, 106, 109, 295]
surviving nonsolo ucounts = 1[295]
ids = [235]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.820 = GAATGAACAAGCTGTT-1

using 139 reads

====================================================================================

graph has 116 edges initially, 4 edges after simplification

total ucounts = 8
nonsolo ucounts = 6[2, 3^2, 7, 48, 74]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.822 = GAATGAACAAGGTTTC-1

using 12598 reads

====================================================================================

graph has 10465 edges initially, 444 edges after simplification

total ucounts = 1177
nonsolo ucounts = 872[2^123, 3^81, 4^61, 5^55, 6^51, 7^51, 8^25, 9^27, 10^26, 11^21, 12^23, 13^17, 14^17, 15^21, 16^12, 17^20, 18^18, 19^12, 20^13, 21^12, 22^9, 23^11, 24^17, 25^11, 26^8, 27^13, 28^10, 29^7, 30^5, 31^10, 32^10, 33^6, 34^4, 35^3, 36^3, 37^8, 38^7, 39^3, 40^3, 41, 42^2, 43^4, 44^3, 45^2, 46^2, 48, 49^4, 50, 51^4, 53, 57, 60^2, 68, 85, 88, 95, 100, 119, 131, 134^2, 447]
surviving nonsolo ucounts = 3[6, 18, 447]
ids = [206, 422, 145]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.829 = GAATGAACACAACTGT-1

using 41 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 1[38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.830 = GAATGAACACACGCTG-1

using 74 reads

====================================================================================

graph has 66 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 6[2^2, 6, 7, 21, 33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.831 = GAATGAACACATGACT-1

using 36 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.837 = GAATGAACAGACGCAA-1

using 198 reads

====================================================================================

graph has 102 edges initially, 4 edges after simplification

total ucounts = 9
nonsolo ucounts = 7[2^2, 3, 9, 11, 42, 127]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.842 = GAATGAACAGGGTATG-1

using 476 reads

====================================================================================

graph has 196 edges initially, 10 edges after simplification

total ucounts = 17
nonsolo ucounts = 14[2^2, 3, 4^2, 9, 10, 11, 19, 28, 63, 67, 92, 159]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.847 = GAATGAACATAGTAAG-1

using 306 reads

====================================================================================

graph has 279 edges initially, 12 edges after simplification

total ucounts = 11
nonsolo ucounts = 10[7, 12, 15, 16^2, 18, 33, 35, 59, 94]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.854 = GAATGAAGTACTTCTT-1

using 66 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[66]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.856 = GAATGAAGTCAATACC-1

using 45 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[4, 41]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.858 = GAATGAAGTCATCGGC-1

using 156 reads

====================================================================================

graph has 130 edges initially, 6 edges after simplification

total ucounts = 11
nonsolo ucounts = 9[3^2, 4, 6, 9, 14, 19, 46, 50]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.860 = GAATGAAGTCCGACGT-1

using 40 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 1[36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.863 = GAATGAAGTCGTGGCT-1

using 141 reads

====================================================================================

graph has 168 edges initially, 6 edges after simplification

total ucounts = 8
nonsolo ucounts = 5[3, 15, 24, 38, 58]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.864 = GAATGAAGTCTAAACC-1

using 129 reads

====================================================================================

graph has 72 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[129]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.867 = GAATGAAGTGATAAGT-1

using 2516 reads

====================================================================================

graph has 1574 edges initially, 28 edges after simplification

total ucounts = 142
nonsolo ucounts = 91[2^31, 3^10, 4^9, 5^3, 6^6, 7^4, 8, 9^4, 10^3, 11, 12^2, 13^2, 16^2, 21^2, 24^2, 27, 28, 37^2, 54, 72, 211, 708, 827]
surviving nonsolo ucounts = 3[211, 708, 827]
ids = [2, 119, 134]

====================================================================================

UMI info for barcode GAATGAAGTGATAAGT-1 contig 1 = GTCATCCCTC...
umi TGTGTATCCT = 717 reads: +391 validated
umi TTGTGGTCTT = 823 reads: +391 validated

GOOD CONTIGS

TIG 1[bases=521]
0-78 ==> 0-78 on |597|TRBV20-1|5'UTR| [len=78] (mis=0)
78-413 ==> 0-335 on |598|TRBV20-1|L-REGION+V-REGION| [len=335] (mis=2)
420-469 ==> 0-49 on |546|TRBJ2-3|J-REGION| [len=49] (mis=0)
469-521 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 2 umis using 88 reads
cdr3 = CSARGGMSTDTQYF at 399, score = 7 + 8
umis assigned: [119, 134]
of which 2 are surviving nonsolos
reads assigned: 1502
start codons at 21, 78, 216, 252, 258, 271, 331, 417
confident = true
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.870 = GAATGAAGTGTAACGG-1

using 304 reads

====================================================================================

graph has 378 edges initially, 26 edges after simplification

total ucounts = 17
nonsolo ucounts = 14[2^3, 4^2, 8^2, 9, 11^2, 43, 53, 62, 82]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.873 = GAATGAAGTTATCGGT-1

using 63 reads

====================================================================================

graph has 58 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 5[2, 3, 7, 25, 26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.876 = GAATGAAGTTGATTGC-1

using 96 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[96]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.877 = GAATGAAGTTGGTGGA-1

using 32 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.879 = GAATGAAGTTTGACAC-1

using 24 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.883 = GAATGAATCACAACGT-1

using 23796 reads

====================================================================================

graph has 12087 edges initially, 480 edges after simplification

total ucounts = 1075
nonsolo ucounts = 838[2^110, 3^78, 4^50, 5^44, 6^35, 7^27, 8^36, 9^23, 10^23, 11^26, 12^26, 13^22, 14^28, 15^19, 16^20, 17^16, 18^15, 19^15, 20^18, 21^18, 22^13, 23^16, 24^10, 25^9, 26^13, 27^12, 28^10, 29^15, 30^8, 31^5, 32^5, 33^3, 34^4, 35^6, 36^5, 37^7, 38^8, 39^4, 40^3, 41, 42^2, 43^2, 44, 45, 46, 47, 48^4, 56, 57, 62, 63, 75, 77^2, 84, 89, 90, 91, 96, 98, 100, 102, 111, 141, 291, 1959, 9296]
surviving nonsolo ucounts = 7[32, 35, 38, 141, 291, 1959, 9296]
ids = [812, 184, 361, 755, 64, 789, 731]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.885 = GAATGAATCACCCGAG-1

using 95 reads

====================================================================================

graph has 52 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[95]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.892 = GAATGAATCAGCACAT-1

using 3795 reads

====================================================================================

graph has 1238 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 4[2^2, 14, 3773]
surviving nonsolo ucounts = 1[3773]
ids = [4]

====================================================================================

UMI info for barcode GAATGAATCAGCACAT-1 contig 1 = TGGGACACTA...
umi CCGTTCATTG = 3788 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=449]
13-351 ==> 0-338 on |530|TRAV9-2|L-REGION+V-REGION| [len=338] (mis=6)
349-407 ==> 0-58 on |420|TRAJ34|J-REGION| [len=58] (mis=1)
407-449 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 1 umis using 258 reads
cdr3 = CALVTYNTDKLIF at 337, score = 9 + 9
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 3712
start codons at 13, 88, 176, 230
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.895 = GAATGAATCAGCTCGG-1

using 25 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 3[2, 3, 17]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.896 = GAATGAATCAGTGCAT-1

using 2687 reads

====================================================================================

graph has 492 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 2[3, 2678]
surviving nonsolo ucounts = 1[2678]
ids = [6]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.898 = GAATGAATCATCGGAT-1

using 8653 reads

====================================================================================

graph has 6956 edges initially, 292 edges after simplification

total ucounts = 759
nonsolo ucounts = 570[2^84, 3^59, 4^35, 5^28, 6^34, 7^18, 8^15, 9^18, 10^21, 11^6, 12^13, 13^19, 14^6, 15^12, 16^13, 17^10, 18^9, 19^12, 20^14, 21^10, 22^5, 23^13, 24^6, 25^3, 26^7, 27^8, 28^8, 29^12, 31^3, 32^6, 33^4, 34^2, 35^2, 36^3, 37^3, 38^3, 39^4, 40^2, 41, 42^4, 43^5, 44^5, 45^5, 47, 48, 49, 50, 51, 53, 55, 60, 61, 62, 66, 68, 71, 73, 88, 90, 101, 113, 114, 115]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.900 = GAATGAATCATTGCCC-1

using 65 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[65]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.901 = GAATGAATCATTGCGA-1

using 45 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[45]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.902 = GAATGAATCCACGAAT-1

using 68 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[11, 57]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.905 = GAATGAATCCCTAACC-1

using 30 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.906 = GAATGAATCCCTGACT-1

using 62 reads

====================================================================================

graph has 52 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 6[2^2, 3^2, 7, 42]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.910 = GAATGAATCCGTAGGC-1

using 54 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[54]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.914 = GAATGAATCCTGCTTG-1

using 362 reads

====================================================================================

graph has 272 edges initially, 8 edges after simplification

total ucounts = 22
nonsolo ucounts = 18[2^4, 3, 4, 5, 6, 8, 9, 10, 14, 20, 27, 47, 58, 69, 70]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.915 = GAATGAATCGAATCCA-1

using 6543 reads

====================================================================================

graph has 5526 edges initially, 148 edges after simplification

total ucounts = 568
nonsolo ucounts = 427[2^54, 3^46, 4^31, 5^13, 6^17, 7^20, 8^14, 9^17, 10^12, 11^18, 12^13, 13^14, 14^11, 15^14, 16^10, 17^10, 18^7, 19^9, 20^8, 21^10, 22^7, 23^3, 24^5, 25^6, 26^6, 27^5, 28^4, 29, 30^5, 31, 32^2, 33^2, 34^4, 37^3, 38, 39, 41, 42^3, 43^2, 44, 45^2, 47, 50, 52, 54, 56^2, 59, 66^2, 75, 87, 91, 107, 630]
surviving nonsolo ucounts = 1[630]
ids = [66]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.924 = GAATGAATCTAACCGA-1

using 11 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[11]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.927 = GAATGAATCTAGCACA-1

using 30 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.928 = GAATGAATCTCGATGA-1

using 696 reads

====================================================================================

graph has 324 edges initially, 10 edges after simplification

total ucounts = 27
nonsolo ucounts = 22[2^7, 4^2, 9, 16, 21, 25, 31, 37, 39, 45, 49, 86, 91, 102, 118]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.929 = GAATGAATCTCTAGGA-1

using 34 reads

====================================================================================

graph has 6 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.933 = GAATGAATCTGGTATG-1

using 29 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.934 = GAATGAATCTGTACGA-1

using 108 reads

====================================================================================

graph has 96 edges initially, 4 edges after simplification

total ucounts = 8
nonsolo ucounts = 6[3, 5, 10, 24, 25, 39]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.937 = GAATGAATCTTGCAAG-1

using 43 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 2[12, 25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.940 = GAATGAATCTTTACGT-1

using 124 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[124]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.941 = GAATGAATCTTTAGTC-1

using 97 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[97]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.942 = GACACGCAGAAACCTA-1

using 56 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[56]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.945 = GACACGCAGAATTCCC-1

using 716 reads

====================================================================================

graph has 169 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 5[2, 4^2, 5, 698]
surviving nonsolo ucounts = 1[698]
ids = [7]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.950 = GACACGCAGACTTTCG-1

using 507 reads

====================================================================================

graph has 326 edges initially, 16 edges after simplification

total ucounts = 24
nonsolo ucounts = 15[2, 3^3, 6, 19, 23, 30, 35, 37, 43, 50, 61, 72, 111]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.956 = GACACGCAGCACACAG-1

using 65 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[65]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.957 = GACACGCAGCACCGCT-1

using 45 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[17, 28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.972 = GACACGCAGGCTCATT-1

using 45 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[45]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.973 = GACACGCAGGGATGGG-1

using 36 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[2, 34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.983 = GACACGCAGTGACTCT-1

using 95 reads

====================================================================================

graph has 198 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[95]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.992 = GACACGCCAAACTGTC-1

using 1883 reads

====================================================================================

graph has 2062 edges initially, 54 edges after simplification

total ucounts = 237
nonsolo ucounts = 148[2^25, 3^18, 4^19, 5^12, 6^4, 7^6, 8^2, 9^4, 10^3, 11^7, 12, 13^4, 14^6, 15^3, 16^2, 17^3, 18, 19^3, 20, 21^3, 22, 23, 24, 26, 29, 30, 32, 33, 34, 35, 36, 38, 41^2, 43, 53, 55, 64, 70, 81, 85]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.995 = GACACGCCAACACGCC-1

using 69 reads

====================================================================================

graph has 52 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 5[3, 4, 5, 10, 46]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.996 = GACACGCCAAGACACG-1

using 38 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.999 = GACACGCCAAGCCTAT-1

using 26 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[2^2, 21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.1000 = GACACGCCAAGCGCTC-1

using 54 reads

====================================================================================

graph has 84 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 5[2^2, 3^2, 40]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.1005 = GACACGCCAATGCCAT-1

using 2234 reads

====================================================================================

graph has 756 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[2, 2230]
surviving nonsolo ucounts = 1[2230]
ids = [3]

====================================================================================

UMI info for barcode GACACGCCAATGCCAT-1 contig 1 = AGAAGACCCC...
umi GCTGACAGCC = 2259 reads: +400 validated

GOOD CONTIGS

TIG 1[bases=482]
0-30 ==> 14-44 on |659|TRBV6-6|5'UTR| [len=44] (mis=0)
30-368 ==> 0-338 on |660|TRBV6-6|L-REGION+V-REGION| [len=344] (mis=2)
379-430 ==> 0-51 on |539|TRBJ1-4|J-REGION| [len=51] (mis=1)
430-482 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 1 umis using 171 reads
cdr3 = CASRGLRAANEKLFF at 357, score = 9 + 8
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 2212
start codons at 30, 88, 141, 165, 180, 207, 271, 385
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.1008 = GACACGCCACACTGCG-1

using 45 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[45]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.1010 = GACACGCCACATCCAA-1

using 116 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 4[3^2, 26, 83]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.1013 = GACACGCCACCAGGTC-1

using 11 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 1[9]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.1018 = GACACGCCACCGTTGG-1

using 14674 reads

====================================================================================

graph has 5490 edges initially, 84 edges after simplification

total ucounts = 598
nonsolo ucounts = 414[2^81, 3^66, 4^36, 5^38, 6^30, 7^19, 8^11, 9^12, 10^11, 11^7, 12^7, 13^12, 14^10, 15^6, 16^7, 17^7, 18^3, 19^3, 20, 21^6, 22^4, 23, 25^2, 26^2, 27^4, 28^4, 29, 30, 32, 33^2, 34^3, 35, 37, 40, 41, 47, 48, 50, 53, 59, 66, 68, 69, 83, 100, 460, 10194]
surviving nonsolo ucounts = 2[460, 10194]
ids = [6, 299]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.1019 = GACACGCCACCTCGTT-1

using 47 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[47]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.1027 = GACACGCCACTCGACG-1

using 8752 reads

====================================================================================

graph has 2482 edges initially, 4 edges after simplification

total ucounts = 11
nonsolo ucounts = 4[2^2, 31, 8710]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================

REJECT CONTIGS

TIG 1[bases=338]
4-237 ==> 1243-1476 on segment before TRAJ35 exon 1 [len=1476] (mis=0)
237-296 ==> 0-59 on |421|TRAJ35|J-REGION| [len=59] (mis=0)
296-338 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
umis assigned: [6]
of which 0 are surviving nonsolos
reads assigned: 8568
start codons at 37, 61, 110, 119, 124, 186, 194, 202, 224, 251
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.1033 = GACACGCCAGCGAACA-1

using 13225 reads

====================================================================================

graph has 5150 edges initially, 54 edges after simplification

total ucounts = 306
nonsolo ucounts = 226[2^44, 3^33, 4^21, 5^19, 6^11, 7^11, 8^20, 9^7, 10^7, 11^6, 12, 13^2, 14^7, 15, 16^2, 17, 18^2, 19^2, 21^2, 22, 23^2, 24^3, 25^2, 26, 29, 33, 35, 38, 43, 46^2, 52, 58, 82, 904, 944, 1434, 1534, 1537, 1571, 1645, 1714]
surviving nonsolo ucounts = 8[904, 944, 1434, 1534, 1537, 1571, 1645, 1714]
ids = [110, 18, 228, 66, 130, 60, 220, 254]

====================================================================================

UMI info for barcode GACACGCCAGCGAACA-1 contig 1 = GAGCTTTTGT...
umi ACCCAGCGGT = 937 reads: +394 validated

UMI info for barcode GACACGCCAGCGAACA-1 contig 2 = GTCATCCCTC...
umi ATCTCTCTCA = 1571 reads: +406 validated
umi ATGCCCTCCA = 1541 reads: +406 validated
umi CCATACCGCC = 895 reads: +406 validated
umi CGACTCCTTC = 1530 reads: +406 validated
umi GTGCTCTTTC = 1663 reads: +197 -1XX +208 invalidated
umi GTTTTCTCGT = 1438 reads: +406 validated
umi TCCCGATGTA = 1713 reads: +406 validated

GOOD CONTIGS

TIG 1[bases=579]
143-484 ==> 0-341 on |522|TRAV8-3|L-REGION+V-REGION| [len=341] (mis=5)
483-537 ==> 9-63 on |401|TRAJ17|J-REGION| [len=63] (mis=0)
537-579 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 1 umis using 26 reads
cdr3 = CAGLTAGNKLTF at 470, score = 7 + 9
umis assigned: [18]
of which 1 are surviving nonsolos
reads assigned: 931
start codons at 143, 265, 279, 306, 453
confident = true

TIG 2[bases=536]
0-78 ==> 0-78 on |597|TRBV20-1|5'UTR| [len=78] (mis=0)
78-413 ==> 0-335 on |598|TRBV20-1|L-REGION+V-REGION| [len=335] (mis=3)
441-484 ==> 7-50 on |540|TRBJ1-5|J-REGION| [len=50] (mis=0)
484-536 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 7 umis using 577 reads
cdr3 = CSASRGARRGRAPGQPQHF at 399, score = 7 + 8
umis assigned: [60, 66, 110, 130, 220, 228, 254]
of which 7 are surviving nonsolos
reads assigned: 10168
start codons at 21, 78, 216, 252, 258, 271, 331, 460
confident = true
now this is a cell
paired!

TCTGTGCATTGGAGTGATGCTGCTGAGTACTTCTGTGCTGGGCTGACTGCAGGCAACAAGCTAACTTTTGGAGGAGGAACCAGGGTGCTAGTTAAACCAA <==> AGCAGCTTCTACATCTGCAGTGCTAGTCGGGGAGCCCGACGCGGCAGGGCTCCTGGACAGCCCCAGCATTTTGGTGATGGGACTCGACTCTCCATCCTAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.1034 = GACACGCCAGCGTTCG-1

using 35 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.1035 = GACACGCCAGCTGCAC-1

using 42 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[42]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.1036 = GACACGCCAGCTGTTA-1

using 25 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.1039 = GACACGCCAGGTCTCG-1

using 23279 reads

====================================================================================

graph has 7415 edges initially, 133 edges after simplification

total ucounts = 547
nonsolo ucounts = 367[2^69, 3^52, 4^37, 5^24, 6^21, 7^19, 8^17, 9^16, 10^9, 11^8, 12^10, 13^7, 14^5, 15^3, 16^6, 17^3, 18^5, 19^3, 20^3, 21^2, 22, 23^4, 24, 25, 27, 28, 30^3, 31^4, 33^3, 34, 35^3, 36^3, 39^2, 42, 46^2, 55, 61, 62, 63^2, 64, 67, 72, 85, 104, 109, 165, 272, 636, 1204, 8337, 8689]
surviving nonsolo ucounts = 5[272, 636, 1204, 8337, 8689]
ids = [201, 257, 395, 17, 314]

====================================================================================

UMI info for barcode GACACGCCAGGTCTCG-1 contig 1 = ATTATTTTTT...
umi CGAACTCCTG = 271 reads: +388 validated
umi GAAGTGCCGT = 619 reads: +388 validated

UMI info for barcode GACACGCCAGGTCTCG-1 contig 2 = TGGGGGCTCA...
umi AACGGTCCTA = 6810 reads: -375 +6 -1XX +1 -1XX +2 -1XX +5 -2XX +1 -1XX +1 invalidated
umi GCGTGAATCC = 7330 reads: -375 +6 -1XX +1 -1XX +2 -1XX +5 -2XX +1 -1XX +1 invalidated
umi TAAGCTGGAT = 1205 reads: +397 validated

GOOD CONTIGS

TIG 1[bases=486]
0-56 ==> 0-56 on |461|TRAV12-3|5'UTR| [len=56] (mis=0)
56-399 ==> 0-343 on |462|TRAV12-3|L-REGION+V-REGION| [len=343] (mis=2)
400-444 ==> 14-58 on |420|TRAJ34|J-REGION| [len=58] (mis=0)
444-486 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 2 umis using 43 reads
cdr3 = CAMKAKLIF at 386, score = 9 + 9
umis assigned: [201, 257]
of which 2 are surviving nonsolos
reads assigned: 880
start codons at 56, 59, 221, 263, 294, 392
confident = true

TIG 2[bases=505]
56-403 ==> 0-347 on |689|TRBV7-9|L-REGION+V-REGION| [len=347] (mis=1)
415-453 ==> 10-48 on |536|TRBJ1-1|J-REGION| [len=48] (mis=0)
453-505 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 1 umis using 78 reads
cdr3 = CASSLDSRDGAFF at 386, score = 7 + 8
umis assigned: [17, 314, 395]
of which 3 are surviving nonsolos
reads assigned: 15328
start codons at 56, 80, 165, 264, 377
confident = true
now this is a cell
paired!

TTCATCAGAGACTCACAGCCCAGTGATTCAGCCACCTACCTCTGTGCAATGAAGGCCAAGCTCATCTTTGGGACTGGGACCAGATTACAAGTCTTTCCAA <==> CGCACAGAGCAGGGGGACTCGGCCATGTATCTCTGTGCCAGCAGCTTAGATTCCAGGGACGGAGCTTTCTTTGGACAAGGCACCAGACTCACAGTTGTAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.1042 = GACACGCCATAAAGGT-1

using 42 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[2, 38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.1043 = GACACGCCATAACCTG-1

using 41047 reads

====================================================================================

graph has 12876 edges initially, 154 edges after simplification

total ucounts = 760
nonsolo ucounts = 519[2^103, 3^69, 4^53, 5^42, 6^33, 7^27, 8^25, 9^20, 10^4, 11^16, 12^9, 13^15, 14^7, 15^10, 16^5, 17^6, 18^7, 19^5, 20^3, 21^2, 22^2, 23^4, 24, 25^2, 26^2, 27, 28, 29, 30^2, 31, 32^4, 33, 35, 36^3, 40, 41, 44, 53, 54, 55, 64, 67, 70, 72, 73, 76, 86, 95, 116, 118, 482, 597, 715, 948, 1000, 1242, 1329, 1361, 1374, 1430, 1467, 1984, 2458, 2914, 7380, 9386]
surviving nonsolo ucounts = 16[482, 597, 715, 948, 1000, 1242, 1329, 1361, 1374, 1430, 1467, 1984, 2458, 2914, 7380, 9386]
ids = [24, 611, 728, 225, 99, 1, 628, 620, 90, 57, ...]

====================================================================================

UMI info for barcode GACACGCCATAACCTG-1 contig 1 = AGTAGCTGTT...
umi AAACACCCTA = 1241 reads: +382 validated
umi ACCAAAACGC = 1419 reads: +382 validated
umi ACTTTTAGGC = 1356 reads: +382 validated
umi AGCCATATGG = 1032 reads: +132 -1XX +249 invalidated
umi CATTCCGCAT = 940 reads: +382 validated
umi TCAGGACTTA = 567 reads: +329 -2 +2 -10 +39 non-validated
umi TCCAGCCGTG = 1358 reads: +382 validated
umi TCCGAAATGT = 1335 reads: +382 validated

UMI info for barcode GACACGCCATAACCTG-1 contig 2 = AGTGACCCTG...
umi CACCCGACAG = 9375 reads: -185X +2 -1XX +209 invalidated
umi CTCGTTGGAC = 1997 reads: -230X +167 invalidated
umi CTTCACCCGT = 2901 reads: -230X +167 invalidated
umi TTCTTTGCCA = 699 reads: +397 validated

GOOD CONTIGS

TIG 1[bases=520]
0-96 ==> 113-209 on |489|TRAV26-1|5'UTR| [len=209] (mis=0)
96-414 ==> 0-318 on |490|TRAV26-1|L-REGION+V-REGION| [len=328] (mis=0)
427-478 ==> 6-57 on |417|TRAJ31|J-REGION| [len=57] (mis=0)
478-520 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 7 umis using 371 reads
cdr3 = CITPSWDNARLMF at 408, score = 8 + 7
umis assigned: [1, 57, 90, 99, 225, 611, 620, 628]
of which 8 are surviving nonsolos
reads assigned: 9057
start codons at 96, 148, 174, 235, 241, 292, 307, 316, 321, 430, 441, 451
confident = true

TIG 2[bases=494]
0-45 ==> 0-45 on |563|TRBV11-3|5'UTR| [len=45] (mis=0)
45-392 ==> 0-347 on |564|TRBV11-3|L-REGION+V-REGION| [len=347] (mis=0)
396-442 ==> 7-53 on |541|TRBJ1-6|J-REGION| [len=53] (mis=0)
442-494 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 4 umis using 1298 reads
cdr3 = CASSLEGNSPLHF at 375, score = 9 + 9
umis assigned: [187, 352, 379, 728]
of which 4 are surviving nonsolos
reads assigned: 14730
start codons at 45, 247, 253
confident = true

REJECT CONTIGS

TIG 1[bases=345]
1-92 ==> 13-104 on segment before TRBJ2-3 exon 1 [len=104] (mis=0)
92-141 ==> 0-49 on |546|TRBJ2-3|J-REGION| [len=49] (mis=0)
141-243 ==> 0-102 on segment before TRBJ2-4 exon 1 [len=102] (mis=0)
293-345 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
umis assigned: [24, 47]
of which 2 are surviving nonsolos
reads assigned: 7700
start codons at 2
confident = false
did not find CDR3

TIG 2[bases=443]
2-240 ==> 5098-5336 on segment before TRAV8-3 exon 1 [len=5336] (mis=0)
240-401 ==> 0-161 on |522|TRAV8-3|L-REGION+V-REGION| [len=341] (mis=0)
401-443 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
umis assigned: [244]
of which 1 are surviving nonsolos
reads assigned: 2428
start codons at 240, 362, 376
confident = false
did not find CDR3

TIG 3[bases=520]
13-84 ==> 0-71 on segment before TRBJ2-5 exon 1 [len=71] (mis=0)
84-132 ==> 0-48 on |548|TRBJ2-5|J-REGION| [len=48] (mis=0)
132-204 ==> 0-72 on segment before TRBJ2-6 exon 1 [len=72] (mis=0)
257-421 ==> 0-164 on segment before TRBJ2-7 exon 1 [len=164] (mis=0)
468-520 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
umis assigned: [692]
of which 1 are surviving nonsolos
reads assigned: 1450
start codons at 400
confident = false
did not find CDR3
now this is a cell
paired!

GCTACGCTGAGAGACACTGCTGTGTACTATTGCATCACCCCGTCCTGGGACAATGCCAGACTCATGTTTGGAGATGGAACTCAGCTGGTGGTGAAGCCCA <==> CCTGCAGAGCTTGGGGACTCGGCCGTGTATCTCTGTGCCAGCAGCTTAGAGGGGAATTCACCCCTCCACTTTGGGAACGGGACCAGGCTCACTGTGACAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.1047 = GACACGCCATCCGGGT-1

using 58 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[58]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.1049 = GACACGCCATGCAACT-1

using 68 reads

====================================================================================

graph has 82 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 7[3, 4, 5, 9, 11, 16, 17]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.1052 = GACACGCCATGGTCTA-1

using 56 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[56]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.1061 = GACACGCGTCCTGCTT-1

using 23 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.1068 = GACACGCTCAACGGGA-1

using 34 reads

====================================================================================

graph has 54 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.1070 = GACACGCTCACAATGC-1

using 33 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.1072 = GACACGCTCACCTTAT-1

using 24 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.1073 = GACACGCTCACTCCTG-1

using 51 reads

====================================================================================

graph has 110 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[51]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.1077 = GACACGCTCAGTGTTG-1

using 107 reads

====================================================================================

graph has 98 edges initially, 4 edges after simplification

total ucounts = 6
nonsolo ucounts = 3[12, 29, 63]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.1082 = GACACGCTCCACTGGG-1

using 68 reads

====================================================================================

graph has 74 edges initially, 4 edges after simplification

total ucounts = 6
nonsolo ucounts = 3[5, 22, 38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.1083 = GACACGCTCCAGAAGG-1

using 50 reads

====================================================================================

graph has 76 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[50]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.1086 = GACACGCTCCGTCAAA-1

using 21 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.1087 = GACACGCTCCGTTGCT-1

using 68 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[68]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 52.1091 = GACACGCTCCTGCTTG-1

using 78 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[78]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!
sorting bam, mem = 0.15
running samtools sort -l 8G -o /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_T_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk52-ucc6a7a72e6/files/contig_bam_sorted.bam /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_T_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk52-ucc6a7a72e6/files/contig_bam.bam

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

96.926 seconds used processing barcodes, peak mem = 0.29
