[stdout]

starting vdj_asm_asm, mem = 0.01
available mem = 829.17 GB
/scratch/etanis/software/cellranger-6.1.2/lib/bin/cr_vdj martian assembly main /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_T_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk46-ucc6a7a72e6 /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_T_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk46-ucc6a7a72e6/files /scratch/etanis/ST-118/multi/dimitri-multi/journal/SC_MULTI_CS.SC_MULTI_CORE.MULTI_GEM_WELL_PROCESSOR.VDJ_T_GEM_WELL_PROCESSOR.SC_VDJ_CONTIG_ASSEMBLER.ASSEMBLE_VDJ.fork0.chnk46.ucc6a7a72e6
n50_n50_rpu = 2159

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.0 = CTCGGGATCTCTTATG-1

using 40933 reads

====================================================================================

graph has 12807 edges initially, 164 edges after simplification

total ucounts = 586
nonsolo ucounts = 416[2^65, 3^42, 4^32, 5^31, 6^23, 7^18, 8^18, 9^12, 10^8, 11^19, 12^16, 13^9, 14^15, 15^7, 16^5, 17^4, 18^9, 19^8, 20^3, 21^4, 22^4, 23^5, 24^4, 25^4, 26^4, 27^4, 28, 29^2, 30^2, 32, 33^3, 35, 38, 39, 43, 46, 49, 50, 55, 56^2, 58, 65, 77, 81, 90, 93, 352, 552, 1130, 1138, 1268, 1685, 1762, 1824, 1837, 1857, 1860, 1906, 1917, 2011, 2124, 2320, 2725, 8102]
surviving nonsolo ucounts = 17[352, 552, 1130, 1138, 1685, 1762, 1824, 1837, 1857, 1860, 1906, 1917, 2011, 2124, 2320, 2725, 8102]
ids = [521, 104, 401, 471, 109, 479, 279, 278, 141, 251, ...]

====================================================================================

UMI info for barcode CTCGGGATCTCTTATG-1 contig 1 = ATGAGATCCT...
umi CAAGAAGACG = 2145 reads: +397 validated
umi CAGGAGTCGG = 1689 reads: +397 validated
umi CCAAAGTCAT = 1159 reads: -377 +1 -3X +3 -12XX +1 invalidated
umi CCAGAAGGCC = 1849 reads: +397 validated
umi CGATCTACTT = 2054 reads: +397 validated
umi CGGGGTTCAA = 2330 reads: +397 validated
umi CTTCATGGGG = 1868 reads: +397 validated
umi GTACTCAAAT = 1924 reads: +397 validated
umi TCTTACCGCA = 1154 reads: +397 validated
umi TCTTTTGATA = 1762 reads: +397 validated
umi TGTTTGTATA = 2724 reads: +397 validated
umi TTACTTTCGA = 356 reads: +397 validated
umi TTTACCCGAG = 1909 reads: +397 validated

UMI info for barcode CTCGGGATCTCTTATG-1 contig 2 = AGATCAGAAG...
umi CACGCGTCGG = 560 reads: +409 validated
umi GATCGCCGAA = 1822 reads: +409 validated
umi GATGATGTCA = 1827 reads: +409 validated
umi TACATGTACT = 6468 reads: -374X +8 -2XX +5 -2XX +1 -2XX +4 -1XX +2 -4XX +2 -2XX invalidated
umi TACTCCCCAG = 1098 reads: +409 validated

GOOD CONTIGS

TIG 1[bases=473]
0-24 ==> 0-24 on |558|TRBV10-3|5'UTR| [len=24] (mis=0)
24-368 ==> 0-344 on |560|TRBV10-3|L-REGION+V-REGION| [len=344] (mis=2)
370-421 ==> 0-51 on |544|TRBJ2-2|J-REGION| [len=51] (mis=3)
421-473 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 12 umis using 1407 reads
cdr3 = CAISEARRTGELFF at 351, score = 10 + 9
umis assigned: [94, 109, 133, 141, 191, 202, 251, 345, 471, 479] and 3 others
of which 12 are surviving nonsolos
reads assigned: 22473
start codons at 0, 24, 46, 78, 82, 146, 174, 202, 229, 265
confident = true

TIG 2[bases=497]
0-46 ==> 0-46 on |506|TRAV38-2/DV8|5'UTR| [len=46] (mis=0)
46-395 ==> 0-349 on |507|TRAV38-2/DV8|L-REGION+V-REGION| [len=349] (mis=4)
392-455 ==> 0-63 on |443|TRAJ57|J-REGION| [len=63] (mis=1)
455-497 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 4 umis using 277 reads
cdr3 = CAYRETQGGSEKLVF at 379, score = 7 + 9
umis assigned: [104, 278, 279, 392, 401]
of which 5 are surviving nonsolos
reads assigned: 11687
start codons at 46, 51, 103, 136, 179, 238, 278, 362, 370
confident = true
now this is a cell
paired!

CTGGGGGATGCCGCGATGTATTTCTGTGCTTATAGGGAAACTCAGGGCGGATCTGAAAAGCTGGTCTTTGGAAAGGGAACGAAACTGACAGTAAACCCAT <==> GCTACCAGCTCCCAGACATCTGTGTACTTCTGTGCCATCAGTGAAGCCCGGCGCACCGGGGAGCTGTTTTTTGGAGAAGGCTCTAGGCTGACCGTACTGG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.3 = CTCGGGATCTTGTACT-1

using 26 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.4 = CTCGGGATCTTTAGTC-1

using 147 reads

====================================================================================

graph has 102 edges initially, 8 edges after simplification

total ucounts = 13
nonsolo ucounts = 11[2^3, 3, 4, 5, 6, 8, 27, 32, 54]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.7 = CTCGTACAGAAGGGTA-1

using 61 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[61]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.11 = CTCGTACAGACTAAGT-1

using 113 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[4, 109]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.13 = CTCGTACAGAGCTGCA-1

using 99 reads

====================================================================================

graph has 56 edges initially, 4 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[26, 72]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.16 = CTCGTACAGAGGTTAT-1

using 37 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.20 = CTCGTACAGATGGGTC-1

using 291 reads

====================================================================================

graph has 276 edges initially, 10 edges after simplification

total ucounts = 33
nonsolo ucounts = 24[2^7, 3^3, 4^2, 7^4, 9, 12, 13, 15^2, 43, 49, 67]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.24 = CTCGTACAGCAGACTG-1

using 58 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[58]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.25 = CTCGTACAGCATCATC-1

using 63 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[63]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.26 = CTCGTACAGCCCAGCT-1

using 50 reads

====================================================================================

graph has 74 edges initially, 2 edges after simplification

total ucounts = 14
nonsolo ucounts = 11[2^5, 3^3, 6, 8, 14]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.32 = CTCGTACAGCTAGTTC-1

using 12 reads

====================================================================================

graph has 4 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[12]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.35 = CTCGTACAGCTGATAA-1

using 49 reads

====================================================================================

graph has 66 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 7[2, 3^2, 4, 5, 8, 21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.36 = CTCGTACAGCTGCAAG-1

using 706 reads

====================================================================================

graph has 498 edges initially, 6 edges after simplification

total ucounts = 40
nonsolo ucounts = 33[2^2, 3^2, 5^5, 7^2, 8, 9^2, 11^2, 13^3, 15, 17^2, 20, 23, 27, 28, 36^2, 43, 46, 65, 89, 101]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.41 = CTCGTACAGGAGCGAG-1

using 1828 reads

====================================================================================

graph has 494 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[1827]
surviving nonsolo ucounts = 1[1827]
ids = [1]

====================================================================================

UMI info for barcode CTCGTACAGGAGCGAG-1 contig 1 = ATCCTGCCCT...
umi TTTAAAGATC = 1824 reads: +397 validated

GOOD CONTIGS

TIG 1[bases=468]
0-19 ==> 20-39 on |672|TRBV7-2|5'UTR| [len=39] (mis=0)
19-366 ==> 0-347 on |673|TRBV7-2|L-REGION+V-REGION| [len=347] (mis=6)
368-416 ==> 0-48 on |548|TRBJ2-5|J-REGION| [len=48] (mis=3)
416-468 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 1 umis using 124 reads
cdr3 = CASSPWKRETQYF at 349, score = 8 + 7
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 1800
start codons at 19, 128
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.45 = CTCGTACAGGGAGTAA-1

using 524 reads

====================================================================================

graph has 220 edges initially, 4 edges after simplification

total ucounts = 7
nonsolo ucounts = 3[13, 32, 475]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.46 = CTCGTACAGGGTTCCC-1

using 32 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.47 = CTCGTACAGGTAAACT-1

using 35 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.48 = CTCGTACAGGTGCACA-1

using 110 reads

====================================================================================

graph has 78 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[110]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.49 = CTCGTACAGTACGACG-1

using 64 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[64]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.51 = CTCGTACAGTAGCCGA-1

using 131 reads

====================================================================================

graph has 154 edges initially, 4 edges after simplification

total ucounts = 13
nonsolo ucounts = 9[2^3, 3, 4, 20, 27, 29, 38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.53 = CTCGTACAGTCGTACT-1

using 57 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[57]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.54 = CTCGTACAGTCTTGCA-1

using 121 reads

====================================================================================

graph has 118 edges initially, 4 edges after simplification

total ucounts = 10
nonsolo ucounts = 9[2^2, 4, 8, 9, 11, 19, 30, 35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.57 = CTCGTACAGTGGAGAA-1

using 439 reads

====================================================================================

graph has 309 edges initially, 6 edges after simplification

total ucounts = 20
nonsolo ucounts = 18[5, 6^3, 7, 9, 14, 15^2, 20, 25, 26, 35, 40, 45, 46^2, 71]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.58 = CTCGTACAGTGTCCAT-1

using 36 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.59 = CTCGTACCAAAGAATC-1

using 28236 reads

====================================================================================

graph has 12618 edges initially, 312 edges after simplification

total ucounts = 1142
nonsolo ucounts = 871[2^120, 3^64, 4^45, 5^43, 6^44, 7^38, 8^30, 9^42, 10^19, 11^26, 12^25, 13^26, 14^19, 15^25, 16^27, 17^23, 18^22, 19^17, 20^17, 21^19, 22^16, 23^12, 24^16, 25^14, 26^12, 27^13, 28^10, 29^5, 30^12, 31^11, 32^7, 33^6, 34^2, 35^3, 36^3, 37^2, 38^3, 39^2, 40^2, 41, 42, 44^3, 45, 46, 47, 49, 51, 57, 62, 66^2, 71, 93, 117, 467, 547, 990, 1030, 1164, 1238, 1472, 1564, 1825, 1893, 2123, 2456]
surviving nonsolo ucounts = 12[117, 467, 990, 1030, 1164, 1238, 1472, 1564, 1825, 1893, 2123, 2456]
ids = [533, 1000, 783, 24, 612, 72, 614, 223, 605, 388, ...]

====================================================================================

UMI info for barcode CTCGTACCAAAGAATC-1 contig 1 = GGGGATCTCT...
umi CCATACACAG = 1898 reads: +397 validated
umi GATACCATAA = 1171 reads: +397 validated
umi GATACGACCA = 1485 reads: +397 validated

UMI info for barcode CTCGTACCAAAGAATC-1 contig 2 = ATTCTTTCTT...
umi AACAGTATCA = 932 reads: -380X +1 -3X +3 -5X +1 -5XX +2 invalidated
umi AATGGGCCCT = 1288 reads: +400 validated
umi ACCAGTTCCT = 2219 reads: -380X +1 -3XX +3 -5XX +1 -5XX +2 invalidated
umi ATAAGACTAT = 1570 reads: +400 validated
umi CACTGTGAGT = 1859 reads: -366 +2 -1X +2 -1XX +2 -1XX +2 -1XX +6 -1XX +4 -1XX +2 -1XX +2 -4XX +1 invalidated
umi CTCTTTAACG = 91 reads: -371 +6 -1X +2 -1XX +2 -2XX +7 -1XX +2 -3XX +2 invalidated
umi GAGGCTCGTA = 1836 reads: +400 validated
umi TAACGCGTAA = 987 reads: +400 validated

GOOD CONTIGS

TIG 1[bases=513]
74-411 ==> 0-337 on |458|TRAV12-1|L-REGION+V-REGION| [len=337] (mis=5)
415-471 ==> 7-63 on |431|TRAJ44|J-REGION| [len=63] (mis=0)
471-513 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 3 umis using 244 reads
cdr3 = CVVSFVGTASKLTF at 398, score = 7 + 9
umis assigned: [388, 612, 614]
of which 3 are surviving nonsolos
reads assigned: 4464
start codons at 13, 71, 74, 171, 278, 300, 306
confident = true

TIG 2[bases=472]
0-20 ==> 0-20 on |612|TRBV28|5'UTR| [len=20] (mis=0)
20-364 ==> 0-344 on |613|TRBV28|L-REGION+V-REGION| [len=344] (mis=5)
373-420 ==> 0-47 on |550|TRBJ2-7|J-REGION| [len=47] (mis=2)
420-472 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 4 umis using 372 reads
cdr3 = CASRGQGIGGYEQYF at 347, score = 6 + 8
umis assigned: [24, 72, 116, 223, 336, 533, 605, 783]
of which 8 are surviving nonsolos
reads assigned: 10629
start codons at 20, 78, 142, 155, 162, 170, 225, 228, 236, 338
confident = true
now this is a cell
paired!

AAGCTCAGTGATTCAGCCACCTACCTCTGTGTGGTCTCTTTCGTCGGCACTGCCAGTAAACTCACCTTTGGGACTGGAACAAGACTTCAGGTCACGCTCG <==> AGCACCAACCAGACATCTATGTACCTCTGTGCCAGCAGAGGACAGGGGATTGGCGGCTACGAGCAGTACTTCGGGCCGGGCACCAGGCTCACGGTCACAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.61 = CTCGTACCAACACCCG-1

using 45 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[45]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.62 = CTCGTACCAAGAAGAG-1

using 38 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.69 = CTCGTACCAAGTAGTA-1

using 144 reads

====================================================================================

graph has 96 edges initially, 6 edges after simplification

total ucounts = 24
nonsolo ucounts = 15[2^5, 5^3, 10, 12, 14, 16, 18, 19, 21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.74 = CTCGTACCACCATCCT-1

using 59 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[59]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.75 = CTCGTACCACGACTCG-1

using 104 reads

====================================================================================

graph has 102 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[104]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.78 = CTCGTACCACGGCTAC-1

using 22 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.82 = CTCGTACCACTGTCGG-1

using 33 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.88 = CTCGTACCAGCCTTGG-1

using 89 reads

====================================================================================

graph has 129 edges initially, 4 edges after simplification

total ucounts = 17
nonsolo ucounts = 11[2^2, 3^4, 4, 10, 15, 17, 21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.89 = CTCGTACCAGCGATCC-1

using 11350 reads

====================================================================================

graph has 7101 edges initially, 148 edges after simplification

total ucounts = 925
nonsolo ucounts = 674[2^117, 3^79, 4^55, 5^54, 6^30, 7^28, 8^37, 9^24, 10^23, 11^19, 12^20, 13^17, 14^16, 15^15, 16^20, 17^13, 18^13, 19^9, 20^6, 21^5, 22^7, 23^7, 24^5, 25^4, 26, 27^5, 28^5, 29^5, 30, 31^6, 32^3, 33^2, 34^3, 35^2, 36^2, 37^2, 38, 39, 40^2, 43, 46, 50, 51, 54^2, 64, 91, 1168, 3275]
surviving nonsolo ucounts = 2[1168, 3275]
ids = [557, 838]

====================================================================================

REJECT CONTIGS

TIG 1[bases=580]
4-83 ==> 25-104 on segment before TRBJ2-3 exon 1 [len=104] (mis=0)
83-132 ==> 0-49 on |546|TRBJ2-3|J-REGION| [len=49] (mis=0)
132-234 ==> 0-102 on segment before TRBJ2-4 exon 1 [len=102] (mis=0)
284-355 ==> 0-71 on segment before TRBJ2-5 exon 1 [len=71] (mis=0)
403-475 ==> 0-72 on segment before TRBJ2-6 exon 1 [len=72] (mis=0)
528-580 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
umis assigned: [557, 838]
of which 2 are surviving nonsolos
reads assigned: 4280
start codons at 
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.90 = CTCGTACCAGCGTAAG-1

using 78 reads

====================================================================================

graph has 88 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 7[7^3, 8, 12, 13, 23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.92 = CTCGTACCAGCTTAAC-1

using 12414 reads

====================================================================================

graph has 7863 edges initially, 200 edges after simplification

total ucounts = 1005
nonsolo ucounts = 786[2^103, 3^79, 4^68, 5^56, 6^45, 7^42, 8^36, 9^39, 10^39, 11^29, 12^24, 13^28, 14^13, 15^15, 16^16, 17^19, 18^15, 19^11, 20^12, 21^8, 22^4, 23^9, 24^7, 25^8, 26^6, 27^9, 28^7, 29^4, 30^5, 31^4, 32^4, 34, 35^2, 36, 39^2, 40, 41, 42, 43, 46, 48, 52, 53, 54, 56, 68, 74, 93, 95, 1097, 2867]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.93 = CTCGTACCAGGACGTA-1

using 39 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 4[3, 6, 8, 19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.96 = CTCGTACCAGTATCTG-1

using 28 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.97 = CTCGTACCAGTATGCT-1

using 18076 reads

====================================================================================

graph has 13263 edges initially, 376 edges after simplification

total ucounts = 1564
nonsolo ucounts = 1243[2^144, 3^118, 4^110, 5^72, 6^74, 7^58, 8^61, 9^52, 10^40, 11^32, 12^32, 13^36, 14^43, 15^46, 16^34, 17^31, 18^34, 19^18, 20^32, 21^26, 22^17, 23^10, 24^11, 25^11, 26^17, 27^8, 28^12, 29^8, 30^6, 31^8, 32^5, 33^7, 34^2, 35^3, 36^3, 37^6, 38^2, 39, 40^2, 42^2, 43^4, 45, 53, 77, 93, 3990]
surviving nonsolo ucounts = 1[3990]
ids = [535]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.99 = CTCGTACCATAAAGGT-1

using 82 reads

====================================================================================

graph has 126 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 7[6^2, 8^2, 11, 14, 25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.103 = CTCGTACCATATACCG-1

using 122 reads

====================================================================================

graph has 90 edges initially, 6 edges after simplification

total ucounts = 9
nonsolo ucounts = 8[2, 4, 10, 14, 16, 21, 23, 31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.107 = CTCGTACCATCTATGG-1

using 93 reads

====================================================================================

graph has 48 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[92]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.111 = CTCGTACCATGTCTCC-1

using 43 reads

====================================================================================

graph has 56 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 5[2^2, 5, 7, 24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.112 = CTCGTACCATTCGACA-1

using 525 reads

====================================================================================

graph has 602 edges initially, 14 edges after simplification

total ucounts = 70
nonsolo ucounts = 47[2^7, 3^4, 4^7, 5, 6^6, 7^3, 8^3, 10^2, 11^3, 12^3, 14, 25^3, 28, 32, 33, 91]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.115 = CTCGTACGTAACGTTC-1

using 56 reads

====================================================================================

graph has 66 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 5[2, 3, 4, 22, 23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.116 = CTCGTACGTAATCGTC-1

using 63 reads

====================================================================================

graph has 98 edges initially, 2 edges after simplification

total ucounts = 13
nonsolo ucounts = 10[2^5, 3, 4, 8, 12, 23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.117 = CTCGTACGTACACCGC-1

using 37 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.118 = CTCGTACGTACCCAAT-1

using 36 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.119 = CTCGTACGTACGAAAT-1

using 115 reads

====================================================================================

graph has 84 edges initially, 4 edges after simplification

total ucounts = 4
nonsolo ucounts = 4[5, 15, 32, 63]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.121 = CTCGTACGTACTTCTT-1

using 265 reads

====================================================================================

graph has 186 edges initially, 8 edges after simplification

total ucounts = 15
nonsolo ucounts = 13[2, 6^2, 8^2, 10, 12, 14, 15, 21, 22, 41, 98]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.122 = CTCGTACGTAGCACGA-1

using 21 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.123 = CTCGTACGTAGCTAAA-1

using 80 reads

====================================================================================

graph has 54 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[2, 78]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.126 = CTCGTACGTATCAGTC-1

using 2612 reads

====================================================================================

graph has 988 edges initially, 6 edges after simplification

total ucounts = 113
nonsolo ucounts = 104[2, 3^2, 4^2, 5^3, 6^3, 7^3, 9^5, 10, 11^3, 12^2, 13^2, 14^3, 15, 16, 17^2, 18, 19^2, 20, 21^2, 22^4, 23^3, 24^5, 25^6, 26^2, 28^4, 29^2, 31^3, 32^4, 33^2, 34^2, 35^2, 36, 37, 38^4, 39^2, 40, 41^2, 42^2, 43^2, 45, 46, 47, 48, 50, 51, 52, 53^2, 56]
surviving nonsolo ucounts = 2[6, 23]
ids = [33, 64]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.129 = CTCGTACGTCAGATAA-1

using 10150 reads

====================================================================================

graph has 8036 edges initially, 254 edges after simplification

total ucounts = 1226
nonsolo ucounts = 895[2^152, 3^82, 4^66, 5^70, 6^48, 7^54, 8^37, 9^28, 10^30, 11^41, 12^27, 13^27, 14^23, 15^17, 16^13, 17^15, 18^20, 19^11, 20^16, 21^9, 22^12, 23^7, 24^12, 25^8, 26^7, 27^6, 28^4, 29^5, 30^7, 31^3, 32^8, 33^4, 34^2, 35, 36^2, 38^3, 41, 43^2, 48, 51, 56, 59^4, 63^2, 71, 75, 78, 81, 91, 363]
surviving nonsolo ucounts = 1[363]
ids = [164]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.132 = CTCGTACGTCCGAGTC-1

using 17 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[17]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.133 = CTCGTACGTCGAACAG-1

using 43 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[3, 7, 32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.135 = CTCGTACGTCGTCTTC-1

using 136 reads

====================================================================================

graph has 80 edges initially, 4 edges after simplification

total ucounts = 7
nonsolo ucounts = 7[2, 3^2, 6, 7, 37, 78]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.136 = CTCGTACGTCGTTGTA-1

using 43 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[43]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.138 = CTCGTACGTCTCCCTA-1

using 52 reads

====================================================================================

graph has 48 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 5[3, 5^2, 14, 24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.139 = CTCGTACGTCTCTCGT-1

using 117 reads

====================================================================================

graph has 110 edges initially, 6 edges after simplification

total ucounts = 5
nonsolo ucounts = 4[3, 27, 34, 52]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.140 = CTCGTACGTCTCTTAT-1

using 67 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[28, 39]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.141 = CTCGTACGTCTGGTCG-1

using 101 reads

====================================================================================

graph has 142 edges initially, 4 edges after simplification

total ucounts = 16
nonsolo ucounts = 12[2^4, 3, 4^2, 7, 12, 17, 20, 22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.143 = CTCGTACGTGCAGGTA-1

using 41 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[41]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.144 = CTCGTACGTGCCTTGG-1

using 42 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[3, 39]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.146 = CTCGTACGTGGGTATG-1

using 28 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.147 = CTCGTACGTGGTCTCG-1

using 22 reads

====================================================================================

graph has 4 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.150 = CTCGTACGTTAAGGGC-1

using 34 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.151 = CTCGTACGTTATCCGA-1

using 432 reads

====================================================================================

graph has 142 edges initially, 4 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[59, 373]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.154 = CTCGTACGTTCTGAAC-1

using 48 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[48]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.155 = CTCGTACGTTCTGTTT-1

using 23 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.156 = CTCGTACGTTGAGGTG-1

using 71 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[71]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.159 = CTCGTACGTTTCCACC-1

using 91 reads

====================================================================================

graph has 68 edges initially, 4 edges after simplification

total ucounts = 7
nonsolo ucounts = 5[2, 13, 16, 20, 38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.160 = CTCGTACGTTTGACTG-1

using 76 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[76]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.162 = CTCGTACTCAAAGTAG-1

using 23 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.163 = CTCGTACTCAACTCTT-1

using 36 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.164 = CTCGTACTCAATAAGG-1

using 22 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 3[4^2, 10]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.165 = CTCGTACTCAATACCG-1

using 24 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.168 = CTCGTACTCACAGGCC-1

using 27 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.169 = CTCGTACTCACATGCA-1

using 58 reads

====================================================================================

graph has 54 edges initially, 4 edges after simplification

total ucounts = 13
nonsolo ucounts = 6[2^2, 4, 6, 15, 22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.172 = CTCGTACTCACTCCTG-1

using 32 reads

====================================================================================

graph has 47 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 5[2, 3, 5, 6, 15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.174 = CTCGTACTCACTGGGC-1

using 19 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.175 = CTCGTACTCACTTATC-1

using 151 reads

====================================================================================

graph has 178 edges initially, 6 edges after simplification

total ucounts = 17
nonsolo ucounts = 14[3, 4, 5, 6^4, 8, 9, 10, 12, 22, 25, 26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.176 = CTCGTACTCAGATAAG-1

using 25 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 3[3, 4, 18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.177 = CTCGTACTCAGCGATT-1

using 123 reads

====================================================================================

graph has 86 edges initially, 8 edges after simplification

total ucounts = 9
nonsolo ucounts = 8[2, 4, 8, 11^2, 24, 29, 33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.181 = CTCGTACTCATACGGT-1

using 58 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[58]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.182 = CTCGTACTCATCGCTC-1

using 68 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[5, 63]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.184 = CTCGTACTCATGTCCC-1

using 88 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[88]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.185 = CTCGTACTCCACGCAG-1

using 10 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[10]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.189 = CTCGTACTCCCTGACT-1

using 25 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[25]
surviving nonsolo ucounts = 1[25]
ids = [0]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.194 = CTCGTACTCCTCATTA-1

using 53 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[53]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.197 = CTCGTACTCGACAGCC-1

using 19 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.202 = CTCGTACTCGCGGATC-1

using 88 reads

====================================================================================

graph has 70 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 6[2, 6^3, 7, 56]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.204 = CTCGTACTCGGCGCTA-1

using 105 reads

====================================================================================

graph has 150 edges initially, 4 edges after simplification

total ucounts = 15
nonsolo ucounts = 11[2^3, 4, 6, 7, 8, 14^2, 19, 23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.205 = CTCGTACTCGGTGTCG-1

using 16001 reads

====================================================================================

graph has 13129 edges initially, 462 edges after simplification

total ucounts = 1789
nonsolo ucounts = 1350[2^163, 3^151, 4^126, 5^82, 6^76, 7^69, 8^70, 9^56, 10^48, 11^53, 12^38, 13^35, 14^36, 15^30, 16^26, 17^29, 18^32, 19^23, 20^18, 21^21, 22^22, 23^8, 24^14, 25^12, 26^5, 27^6, 28^4, 29^5, 30^8, 31^3, 32^3, 33^8, 34^5, 35^4, 36^4, 37^2, 38^3, 40, 41^4, 42^3, 43^2, 44^4, 45, 46, 48^3, 49^2, 50^2, 51^2, 52^4, 53, 54, 56, 59, 64^2, 65, 66^3, 69, 70, 72^2, 77, 78^2, 80, 83, 84, 96, 108, 112]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.206 = CTCGTACTCGGTGTTA-1

using 20 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.209 = CTCGTACTCGTGGACC-1

using 89 reads

====================================================================================

graph has 122 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 8[2^2, 4, 6, 9, 10, 19, 34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.213 = CTCGTACTCTCGCTTG-1

using 79 reads

====================================================================================

graph has 81 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 8[2^3, 3, 4, 5, 21, 37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.217 = CTCGTACTCTGGTGTA-1

using 29 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.218 = CTCGTACTCTTGCAAG-1

using 113 reads

====================================================================================

graph has 128 edges initially, 8 edges after simplification

total ucounts = 12
nonsolo ucounts = 9[2, 3^2, 4, 8, 15, 23, 24, 28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.220 = CTCGTACTCTTTACAC-1

using 44 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 5[2^2, 4, 7, 26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.222 = CTCGTCAAGACAAAGG-1

using 40 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[40]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.224 = CTCGTCAAGACCTAGG-1

using 24 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.225 = CTCGTCAAGACGACGT-1

using 51 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[2, 20, 28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.226 = CTCGTCAAGACGCACA-1

using 43759 reads

====================================================================================

graph has 14486 edges initially, 138 edges after simplification

total ucounts = 596
nonsolo ucounts = 429[2^80, 3^57, 4^35, 5^23, 6^27, 7^21, 8^18, 9^17, 10^11, 11^16, 12^7, 13^10, 14^11, 15^5, 16^6, 17^4, 18^5, 19^4, 20, 21^3, 22^3, 23^3, 24^2, 25, 26^3, 27, 29^2, 31, 32^3, 33^2, 34, 35, 36^3, 37^2, 38^3, 39^2, 40, 42, 43^3, 45, 46, 47, 54, 76, 80^2, 82, 127, 245, 777, 973, 1075, 1106, 1289, 1347, 1382, 1384, 1395, 1695, 1758, 1829, 1943, 2091, 2141, 2285, 2296, 2514, 4801, 5077]
surviving nonsolo ucounts = 21[245, 777, 973, 1075, 1106, 1289, 1347, 1382, 1384, 1395, 1695, 1758, 1829, 1943, 2091, 2141, 2285, 2296, 2514, 4801, 5077]
ids = [80, 344, 572, 204, 246, 25, 51, 77, 519, 81, ...]

====================================================================================

UMI info for barcode CTCGTCAAGACGCACA-1 contig 1 = AGTCAACTTC...
umi AACGAGCTAG = 1275 reads: +409 validated
umi ACCGTTCTGG = 1393 reads: +409 validated
umi ATCCTGGATC = 1832 reads: +409 validated
umi CTAAATAAGC = 1692 reads: +409 validated
umi CTTATTCTCA = 1776 reads: +409 validated
umi TCATACGGTT = 2173 reads: +110 -1XX +298 invalidated
umi TTCCTTGGTG = 1956 reads: +409 validated
umi TTCGATCAAT = 1010 reads: +409 validated

UMI info for barcode CTCGTCAAGACGCACA-1 contig 2 = ATGTTAAGGA...
umi ACGCCTCCGC = 1405 reads: +394 validated
umi ATAAATGGGC = 2305 reads: +394 validated
umi TAGCTTCTGC = 2134 reads: +394 validated
umi TCATAGACAA = 2514 reads: +394 validated
umi TCGTCCTGGG = 2308 reads: +394 validated
umi TGCATAAGTG = 1382 reads: +394 validated

UMI info for barcode CTCGTCAAGACGCACA-1 contig 3 = ACTCCCACCT...
umi AAACTGTACC = 4231 reads: -379 +1 -1XX +4 -1XX +3 -2XX +1 -1XX +2 -2XX +1 -1XX +1 invalidated
umi ACAAGTTCTT = 1240 reads: -382X +1 -3X +3 -2XX +1 -1XX +2 -2XX +3 invalidated
umi ACGCCGTTAA = 224 reads: -399X +1 invalidated
umi CATGGCATTA = 1076 reads: +400 validated
umi CCGATGATGC = 1063 reads: +400 validated
umi GACAACCTAT = 763 reads: +400 validated
umi GTTATCAGGG = 4172 reads: -371X +1 -3XX +1 -10XX +1 -12XX +1 invalidated

GOOD CONTIGS

TIG 1[bases=487]
0-36 ==> 0-36 on |455|TRAV10|5'UTR| [len=36] (mis=0)
36-379 ==> 0-343 on |456|TRAV10|L-REGION+V-REGION| [len=343] (mis=1)
382-445 ==> 0-63 on |431|TRAJ44|J-REGION| [len=63] (mis=3)
445-487 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 8 umis using 759 reads
cdr3 = CVVSAGHTGTASKLTF at 366, score = 6 + 9
umis assigned: [25, 77, 147, 288, 328, 484, 570, 572]
of which 8 are surviving nonsolos
reads assigned: 12735
start codons at 36, 94, 164, 246, 298
confident = true

TIG 2[bases=477]
0-41 ==> 15-56 on |529|TRAV9-2|5'UTR| [len=56] (mis=0)
41-379 ==> 0-338 on |530|TRAV9-2|L-REGION+V-REGION| [len=338] (mis=6)
375-435 ==> 0-60 on |397|TRAJ12|J-REGION| [len=60] (mis=2)
435-477 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 6 umis using 759 reads
cdr3 = CALKMDSSYKLIF at 365, score = 9 + 9
umis assigned: [81, 132, 456, 485, 497, 519]
of which 6 are surviving nonsolos
reads assigned: 11829
start codons at 0, 41, 116, 204, 258, 377
confident = true

TIG 3[bases=529]
0-77 ==> 0-77 on |578|TRBV15|5'UTR| [len=77] (mis=0)
77-421 ==> 0-344 on |579|TRBV15|L-REGION+V-REGION| [len=344] (mis=2)
426-477 ==> 0-51 on |539|TRBJ1-4|J-REGION| [len=51] (mis=5)
477-529 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 3 umis using 122 reads
cdr3 = CATSRPTGIIEKLFF at 404, score = 6 + 8
umis assigned: [9, 51, 80, 204, 246, 344, 427]
of which 7 are surviving nonsolos
reads assigned: 12663
start codons at 43, 58, 77, 101, 129, 135, 140, 227, 282, 300, 395
confident = true
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.228 = CTCGTCAAGAGCTATA-1

using 22 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[2, 18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.232 = CTCGTCAAGATGCGAC-1

using 69 reads

====================================================================================

graph has 72 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 4[2, 5, 28, 31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.233 = CTCGTCAAGCCAGAAC-1

using 57 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[57]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.235 = CTCGTCAAGCCTTGAT-1

using 84 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[84]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.238 = CTCGTCAAGCGCCTCA-1

using 5579 reads

====================================================================================

graph has 4567 edges initially, 148 edges after simplification

total ucounts = 665
nonsolo ucounts = 471[2^97, 3^65, 4^42, 5^34, 6^36, 7^19, 8^20, 9^10, 10^8, 11^14, 12^13, 13^9, 14^6, 15^4, 16^3, 17^4, 18^5, 19^3, 20^2, 21^6, 22^7, 23^6, 24^5, 25, 26^5, 27^4, 28^2, 29^3, 30, 31, 32^2, 33^3, 35^3, 36, 37^3, 38^3, 41, 42, 43^2, 44, 46, 47^2, 50, 51, 53, 55, 64, 66, 67^2, 82, 84^2, 100, 395]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.244 = CTCGTCAAGCTGCAAG-1

using 15 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.250 = CTCGTCAAGGACAGCT-1

using 35 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.257 = CTCGTCAAGGTAAACT-1

using 25 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.260 = CTCGTCAAGTACCGGA-1

using 43 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 6[2, 3^2, 6, 7, 19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.264 = CTCGTCAAGTGCTGCC-1

using 25 reads

====================================================================================

graph has 4 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.267 = CTCGTCAAGTTCGATC-1

using 48 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[48]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.273 = CTCGTCACAAAGGTGC-1

using 9602 reads

====================================================================================

graph has 6457 edges initially, 138 edges after simplification

total ucounts = 740
nonsolo ucounts = 555[2^93, 3^64, 4^63, 5^45, 6^44, 7^29, 8^28, 9^29, 10^22, 11^20, 12^10, 13^12, 14^6, 15^11, 16^7, 17^2, 18^3, 19^3, 20^7, 21^5, 22^5, 23, 24^2, 25^2, 26, 28^4, 30^3, 31^2, 32^2, 33, 34, 35, 36, 38, 42, 43, 46, 47, 48, 49^2, 50, 52, 53^2, 60^2, 61, 67, 70^2, 90, 132, 189, 433, 472, 596, 927, 1623]
surviving nonsolo ucounts = 6[189, 433, 472, 596, 927, 1623]
ids = [440, 419, 109, 353, 699, 420]

====================================================================================

UMI info for barcode CTCGTCACAAAGGTGC-1 contig 1 = TTCCTCTGCT...
umi ACTGAAAGGG = 448 reads: +379 validated
umi GCGAAACACT = 1615 reads: +379 validated
umi GCTTTTTCTA = 172 reads: +332 -40 +1 -1 +5 non-validated
umi TAAGGACTCA = 1 reads: -379X invalidated
umi TTCTATGTTA = 918 reads: +379 validated

GOOD CONTIGS

TIG 1[bases=543]
0-112 ==> 0-112 on |618|TRBV30|5'UTR| [len=112] (mis=0)
112-447 ==> 0-335 on |619|TRBV30|L-REGION+V-REGION| [len=335] (mis=4)
443-491 ==> 0-48 on |536|TRBJ1-1|J-REGION| [len=48] (mis=0)
491-543 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 3 umis using 157 reads
cdr3 = CAWMNTEAFF at 433, score = 7 + 8
umis assigned: [109, 420, 440, 506, 699]
of which 4 are surviving nonsolos
reads assigned: 3109
start codons at 109, 112, 180, 442
confident = true
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.276 = CTCGTCACAACCGCCA-1

using 56 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[55]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.278 = CTCGTCACAAGCCGCT-1

using 15 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.280 = CTCGTCACAAGGTGTG-1

using 39 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[39]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.281 = CTCGTCACAAGTTAAG-1

using 35 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.282 = CTCGTCACAAGTTCTG-1

using 5979 reads

====================================================================================

graph has 5577 edges initially, 166 edges after simplification

total ucounts = 673
nonsolo ucounts = 526[2^88, 3^37, 4^37, 5^34, 6^25, 7^25, 8^20, 9^23, 10^18, 11^20, 12^23, 13^19, 14^18, 15^14, 16^20, 17^15, 18^14, 19^10, 20^8, 21^4, 22^7, 23^8, 24^7, 25^2, 26^3, 27, 28^2, 29, 30^3, 32, 33, 35, 36, 37, 40, 42^2, 43, 47^3, 55, 56, 60, 62, 63^2, 100, 113]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.284 = CTCGTCACAATAGCAA-1

using 32 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 5[2^2, 3, 4, 19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.286 = CTCGTCACAATGGATA-1

using 28 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.291 = CTCGTCACACCAGGTC-1

using 12 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[12]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.293 = CTCGTCACACGACTCG-1

using 16495 reads

====================================================================================

graph has 8188 edges initially, 136 edges after simplification

total ucounts = 686
nonsolo ucounts = 492[2^93, 3^58, 4^40, 5^36, 6^34, 7^21, 8^18, 9^22, 10^15, 11^20, 12^10, 13^14, 14^6, 15^11, 16^9, 17^12, 18^8, 19^6, 20^2, 21^9, 22^6, 24^3, 25, 26^2, 27, 28^3, 29^4, 34^2, 35, 36^2, 37^4, 41^3, 42, 44^2, 52, 70^2, 71, 73, 77, 82, 900, 1461, 1875, 2013, 2473, 2851]
surviving nonsolo ucounts = 6[900, 1461, 1875, 2013, 2473, 2851]
ids = [440, 271, 625, 618, 287, 198]

====================================================================================

UMI info for barcode CTCGTCACACGACTCG-1 contig 1 = AAAGCAGATT...
umi CCTGATTTAC = 1466 reads: +403 validated
umi GGCGATGATA = 901 reads: +403 validated

UMI info for barcode CTCGTCACACGACTCG-1 contig 2 = ACTTCTTAGT...
umi CAAAGCTGGG = 2950 reads: +34 -1XX +1 -5XX +1 -4XX +1 -2XX +1 -2XX +2 -2XX +2 -3XX +1 -2XX +327 invalidated
umi CGCGCCGGGA = 2478 reads: +391 validated
umi TGGATTCAAG = 2017 reads: +391 validated
umi TGGTCAGCTA = 1884 reads: +391 validated

GOOD CONTIGS

TIG 1[bases=550]
0-105 ==> 0-105 on |459|TRAV12-2|5'UTR| [len=105] (mis=0)
105-445 ==> 0-340 on |460|TRAV12-2|L-REGION+V-REGION| [len=340] (mis=2)
453-508 ==> 11-66 on |432|TRAJ45|J-REGION| [len=66] (mis=0)
508-550 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 2 umis using 87 reads
cdr3 = CAVKRKGGGADGLTF at 432, score = 9 + 8
umis assigned: [271, 440]
of which 2 are surviving nonsolos
reads assigned: 2331
start codons at 16, 102, 105, 309, 325, 340, 379
confident = true

TIG 2[bases=486]
0-43 ==> 24-67 on |614|TRBV29-1|5'UTR| [len=67] (mis=0)
43-378 ==> 0-335 on |615|TRBV29-1|L-REGION+V-REGION| [len=335] (mis=2)
384-434 ==> 0-50 on |540|TRBJ1-5|J-REGION| [len=50] (mis=3)
434-486 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 4 umis using 698 reads
cdr3 = CSVAGQGGDQPQHF at 364, score = 7 + 8
umis assigned: [198, 287, 618, 625]
of which 4 are surviving nonsolos
reads assigned: 9075
start codons at 43, 178, 181, 257, 334, 410
confident = true
now this is a cell
paired!

CCCAGTGATTCAGCCACCTACCTCTGTGCCGTGAAGCGAAAGGGCGGAGGTGCTGACGGACTCACCTTTGGCAAAGGGACTCATCTAATCATCCAGCCCT <==> ATGAGCCCTGAAGACAGCAGCATATATCTCTGCAGCGTTGCAGGGCAGGGTGGGGATCAGCCCCAGCATTTTGGTGATGGGACTCGACTCTCCATCCTAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.297 = CTCGTCACACTGAAGG-1

using 68 reads

====================================================================================

graph has 58 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[68]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.300 = CTCGTCACAGATTGCT-1

using 20 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.307 = CTCGTCACAGGGATTG-1

using 28 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 4[2^3, 22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.308 = CTCGTCACAGGTCTCG-1

using 129 reads

====================================================================================

graph has 84 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 3[2, 5, 119]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.313 = CTCGTCACATACAGCT-1

using 40 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[40]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.317 = CTCGTCACATCCGGGT-1

using 49 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[49]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.321 = CTCGTCACATGGTCTA-1

using 54 reads

====================================================================================

graph has 60 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[54]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.324 = CTCGTCACATTCACTT-1

using 58 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[58]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.325 = CTCGTCACATTCGACA-1

using 47 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 2[3, 39]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.330 = CTCGTCAGTAAACACA-1

using 62 reads

====================================================================================

graph has 68 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 6[2, 7, 8, 10, 16, 18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.343 = CTCGTCAGTAGGGTAC-1

using 249 reads

====================================================================================

graph has 124 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[249]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.344 = CTCGTCAGTATATGGA-1

using 65 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[65]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.346 = CTCGTCAGTATTCGTG-1

using 16 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[16]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.347 = CTCGTCAGTATTCTCT-1

using 40 reads

====================================================================================

graph has 6 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[40]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.349 = CTCGTCAGTCATCCCT-1

using 52 reads

====================================================================================

graph has 48 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[52]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.358 = CTCGTCAGTCTCCATC-1

using 27 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.359 = CTCGTCAGTCTCGTTC-1

using 38 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[18, 20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.360 = CTCGTCAGTCTCTCGT-1

using 40688 reads

====================================================================================

graph has 19148 edges initially, 845 edges after simplification

total ucounts = 1311
nonsolo ucounts = 1109[2^91, 3^70, 4^57, 5^54, 6^34, 7^42, 8^24, 9^25, 10^19, 11^18, 12^26, 13^18, 14^18, 15^22, 16^12, 17^7, 18^12, 19^9, 20^7, 21^14, 22^15, 23^17, 24^13, 25^11, 26^12, 27^13, 28^16, 29^8, 30^16, 31^11, 32^12, 33^12, 34^11, 35^7, 36^6, 37^9, 38^12, 39^6, 40^6, 41^6, 42^12, 43^4, 44^7, 45^8, 46^8, 47^6, 48^5, 49^5, 50^6, 51^2, 52^7, 53^10, 54^6, 55^3, 56^8, 57^6, 58^12, 59^4, 60^6, 61^5, 62^7, 63^9, 64^2, 65^6, 66^2, 67^5, 68^2, 69^10, 70^8, 71, 72^5, 73^3, 74, 75^6, 76^3, 77^3, 78^6, 79^8, 80^5, 81^5, 82^3, 83^6, 85^2, 86^5, 87^4, 88^2, 89^4, 90^2, 91^4, 92^2, 93^2, 94^4, 95^3, 96^3, 97, 98^5, 99^6, 101^2, 102^4, 103^2, 105, 106, 108^2, 110, 111^2, 113, 114^2, 118, 123^2, 125, 908, 1284, 1482, 4529]
surviving nonsolo ucounts = 4[908, 1284, 1482, 4529]
ids = [528, 1175, 1063, 1244]

====================================================================================

UMI info for barcode CTCGTCAGTCTCTCGT-1 contig 1 = ACAACTCTCC...
umi CGTGGCAATG = 910 reads: +388 validated
umi CTAGACAGTG = 2 reads: -386X +1 -1XX invalidated
umi GGATCGCTAT = 1 reads: -385X +1 -2X invalidated
umi GGTAATTGGG = 1 reads: -386X +1 -1X invalidated
umi TCTGTGGTGA = 1480 reads: +388 validated
umi TGTATCTGAT = 1 reads: -388X invalidated
umi TTAAGTTTCG = 1272 reads: +388 validated
umi TTGCTCGGAT = 3867 reads: -351 +5 -1XX +2 -1XX +2 -1XX +2 -1XX +6 -1XX +4 -1XX +2 -1XX +2 -4XX +1 invalidated
umi TTGTTCTGGT = 1 reads: -385X +3 invalidated
umi TTTTATAGGG = 2 reads: -385X +1 -2XX invalidated

GOOD CONTIGS

TIG 1[bases=482]
0-42 ==> 36-78 on |597|TRBV20-1|5'UTR| [len=78] (mis=0)
42-377 ==> 0-335 on |598|TRBV20-1|L-REGION+V-REGION| [len=335] (mis=4)
383-430 ==> 0-47 on |550|TRBJ2-7|J-REGION| [len=47] (mis=4)
430-482 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 3 umis using 215 reads
cdr3 = CSADGRGRYEQYF at 363, score = 7 + 8
umis assigned: [528, 545, 760, 785, 1063, 1152, 1175, 1244, 1262, 1297]
of which 4 are surviving nonsolos
reads assigned: 7452
start codons at 42, 180, 216, 222, 235, 295
confident = true
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.363 = CTCGTCAGTCTCTTTA-1

using 18 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.367 = CTCGTCAGTGAAAGAG-1

using 83 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[83]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.372 = CTCGTCAGTGCCTGTG-1

using 72 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[72]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.374 = CTCGTCAGTGCTAGCC-1

using 33 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.375 = CTCGTCAGTGCTTCTC-1

using 31 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[6, 25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 46.376 = CTCGTCAGTGGTCTCG-1

using 74 reads

====================================================================================

graph has 68 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[74]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!
sorting bam, mem = 0.10
running samtools sort -l 8G -o /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_T_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk46-ucc6a7a72e6/files/contig_bam_sorted.bam /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_T_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk46-ucc6a7a72e6/files/contig_bam.bam

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

40.987 seconds used processing barcodes, peak mem = 0.26
