[stdout]

starting vdj_asm_asm, mem = 0.01
available mem = 829.66 GB
/scratch/etanis/software/cellranger-6.1.2/lib/bin/cr_vdj martian assembly main /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_T_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk45-ucc6a7a72e6 /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_T_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk45-ucc6a7a72e6/files /scratch/etanis/ST-118/multi/dimitri-multi/journal/SC_MULTI_CS.SC_MULTI_CORE.MULTI_GEM_WELL_PROCESSOR.VDJ_T_GEM_WELL_PROCESSOR.SC_VDJ_CONTIG_ASSEMBLER.ASSEMBLE_VDJ.fork0.chnk45.ucc6a7a72e6
n50_n50_rpu = 2159

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.0 = CTCCTAGTCTCTAAGG-1

using 432 reads

====================================================================================

graph has 276 edges initially, 16 edges after simplification

total ucounts = 24
nonsolo ucounts = 21[2^2, 3^4, 5, 6^2, 7, 8, 10, 11, 30, 31, 32, 33, 39, 46, 56, 93]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.2 = CTCCTAGTCTGCTGTC-1

using 27 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.5 = CTCCTAGTCTTCAACT-1

using 95 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[95]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.8 = CTCCTAGTCTTGGGTA-1

using 77 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[76]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.10 = CTCCTAGTCTTTAGGG-1

using 65 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[65]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.11 = CTCGAAAAGAAACCTA-1

using 24 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.13 = CTCGAAAAGACAATAC-1

using 13595 reads

====================================================================================

graph has 7834 edges initially, 250 edges after simplification

total ucounts = 634
nonsolo ucounts = 492[2^65, 3^40, 4^25, 5^28, 6^19, 7^20, 8^19, 9^20, 10^12, 11^7, 12^17, 13^14, 14^8, 15^11, 16^11, 17^7, 18^14, 19^12, 20^12, 21^9, 22^7, 23^8, 24^11, 25^10, 26^7, 27^8, 28^5, 29^3, 30^5, 31^6, 32^3, 33^3, 34^3, 35^4, 36^7, 37^4, 38^5, 39^2, 41^4, 42^3, 43, 45, 48^2, 49, 53^2, 55^3, 83, 85, 105, 6328]
surviving nonsolo ucounts = 1[6328]
ids = [272]

====================================================================================

REJECT CONTIGS

TIG 1[bases=357]
0-104 ==> 0-104 on segment before TRBJ2-3 exon 1 [len=104] (mis=4)
104-153 ==> 0-49 on |546|TRBJ2-3|J-REGION| [len=49] (mis=0)
153-255 ==> 0-102 on segment before TRBJ2-4 exon 1 [len=102] (mis=1)
305-357 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
umis assigned: [272]
of which 1 are surviving nonsolos
reads assigned: 6242
start codons at 14
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.15 = CTCGAAAAGACCTTTG-1

using 37 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.24 = CTCGAAAAGATGCGAC-1

using 25 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 1[23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.25 = CTCGAAAAGCAACGGT-1

using 36 reads

====================================================================================

graph has 6 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.27 = CTCGAAAAGCCACCTG-1

using 1595 reads

====================================================================================

graph has 870 edges initially, 26 edges after simplification

total ucounts = 62
nonsolo ucounts = 51[2^2, 3^2, 4, 5^2, 6^4, 7^2, 8^2, 9, 10^2, 12^2, 13^2, 15, 16, 19, 20, 22^2, 23, 28^2, 31, 32, 36, 38, 41, 42^2, 45^2, 46, 52, 55, 59, 66, 81, 82, 91, 108, 115, 127]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.30 = CTCGAAAAGCCTATGT-1

using 49 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[49]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.35 = CTCGAAAAGGAATTAC-1

using 141 reads

====================================================================================

graph has 96 edges initially, 4 edges after simplification

total ucounts = 7
nonsolo ucounts = 5[6, 10, 11^2, 101]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.45 = CTCGAAAAGGGTTCCC-1

using 80000 reads

====================================================================================

graph has 31345 edges initially, 1018 edges after simplification

total ucounts = 1789
nonsolo ucounts = 1534[2^103, 3^88, 4^76, 5^54, 6^70, 7^36, 8^32, 9^40, 10^37, 11^29, 12^30, 13^23, 14^23, 15^23, 16^19, 17^24, 18^30, 19^25, 20^23, 21^17, 22^17, 23^17, 24^21, 25^14, 26^25, 27^15, 28^17, 29^21, 30^12, 31^11, 32^12, 33^11, 34^10, 35^14, 36^8, 37^9, 38^10, 39^14, 40^15, 41^14, 42^15, 43^14, 44^8, 45^11, 46^11, 47^14, 48^11, 49^15, 50^10, 51^9, 52^9, 53^7, 54^8, 55^4, 56^9, 57^8, 58^8, 59^3, 60^7, 61^4, 62^4, 63^3, 64^9, 65^8, 66^6, 67^8, 68^6, 69^6, 70^9, 71^5, 72^8, 73^13, 74^7, 75^7, 76^5, 77^8, 78, 79^4, 80^6, 81^9, 82^4, 83^3, 84^5, 85^5, 86^7, 87^4, 88^4, 89^3, 90^6, 91^7, 92^5, 93^4, 94^2, 95^2, 96, 97^3, 98^2, 99^2, 100^6, 101^5, 102^3, 103^2, 105^2, 106^2, 107^2, 109^3, 110^2, 112, 113^3, 115, 116^2, 117^2, 118^2, 121, 123, 126^2, 129, 132, 136, 144, 151, 156, 419, 1362, 1456, 1947, 1994, 2406, 2432, 2468, 2646, 2889, 13846]
surviving nonsolo ucounts = 11[419, 1362, 1456, 1947, 1994, 2406, 2432, 2468, 2646, 2889, 13846]
ids = [691, 886, 607, 1170, 721, 1515, 1680, 732, 1662, 192, ...]

====================================================================================

UMI info for barcode CTCGAAAAGGGTTCCC-1 contig 1 = ATTCTTTCTT...
umi ACCCGGGTCC = 2902 reads: +397 validated
umi ACTTTTCGTC = 1 reads: -394X +1 -2X invalidated
umi CCACAAACCA = 1 reads: -396X +1 invalidated
umi CCCCACCTGG = 1 reads: -397X invalidated
umi CCCCCATGTG = 1464 reads: +397 validated
umi CCTCAGCCGC = 423 reads: +397 validated
umi CGAAAATGGC = 2015 reads: +397 validated
umi CGAGTGCCAT = 2485 reads: +397 validated
umi CTTAATATTT = 1365 reads: +397 validated
umi GAGACTCACG = 1 reads: -397X invalidated
umi GGGTAGTTTT = 1955 reads: +397 validated
umi TCTCTGCTCT = 2406 reads: +397 validated
umi TTATCATGCA = 2652 reads: +397 validated
umi TTCCATCGCG = 2435 reads: +397 validated

GOOD CONTIGS

TIG 1[bases=469]
0-20 ==> 0-20 on |612|TRBV28|5'UTR| [len=20] (mis=0)
20-356 ==> 0-336 on |613|TRBV28|L-REGION+V-REGION| [len=344] (mis=0)
369-417 ==> 0-48 on |548|TRBJ2-5|J-REGION| [len=48] (mis=3)
417-469 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 10 umis using 1468 reads
cdr3 = CASERVDREETQYF at 347, score = 6 + 7
umis assigned: [192, 281, 567, 603, 607, 691, 721, 732, 886, 967] and 4 others
of which 10 are surviving nonsolos
reads assigned: 19798
start codons at 20, 78, 142, 155, 162, 170, 225, 228, 236, 338
confident = true
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.50 = CTCGAAAAGTAGCGGT-1

using 1223 reads

====================================================================================

graph has 492 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[1223]
surviving nonsolo ucounts = 1[1223]
ids = [0]

====================================================================================

UMI info for barcode CTCGAAAAGTAGCGGT-1 contig 1 = TGGGGGCCCT...
umi TGCGTCGTTC = 1231 reads: +397 validated

GOOD CONTIGS

TIG 1[bases=468]
19-366 ==> 0-347 on |673|TRBV7-2|L-REGION+V-REGION| [len=347] (mis=1)
368-416 ==> 0-48 on |537|TRBJ1-2|J-REGION| [len=48] (mis=4)
416-468 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 1 umis using 107 reads
cdr3 = CASSLVGVYGYTF at 349, score = 8 + 9
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 1205
start codons at 19, 128, 374
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.53 = CTCGAAAAGTCGTTTG-1

using 6418 reads

====================================================================================

graph has 5457 edges initially, 235 edges after simplification

total ucounts = 704
nonsolo ucounts = 513[2^79, 3^55, 4^34, 5^31, 6^27, 7^20, 8^24, 9^25, 10^16, 11^19, 12^14, 13^10, 14^11, 15^16, 16^10, 17^9, 18^10, 19^5, 20^4, 21^11, 22^10, 23^3, 24^5, 25^9, 26^8, 27^6, 28^4, 29^3, 30^6, 31^2, 34^2, 35, 37^3, 38, 39^2, 40^3, 50, 51, 54, 56, 57, 59, 62, 63, 64, 66, 70, 85, 88, 94, 120]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.55 = CTCGAAAAGTGCCAGA-1

using 63 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[63]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.56 = CTCGAAAAGTGCGTGA-1

using 41 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[41]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.57 = CTCGAAAAGTGTACTC-1

using 62 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[62]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.60 = CTCGAAACAAACAACA-1

using 24 reads

====================================================================================

graph has 4 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.63 = CTCGAAACAAATTGCC-1

using 4934 reads

====================================================================================

graph has 4974 edges initially, 156 edges after simplification

total ucounts = 583
nonsolo ucounts = 435[2^54, 3^46, 4^43, 5^26, 6^24, 7^21, 8^25, 9^18, 10^12, 11^18, 12^12, 13^10, 14^14, 15^14, 16^6, 17^9, 18^8, 19^10, 20^5, 21^8, 22^7, 23^8, 24^5, 25^4, 26^3, 28^5, 29^2, 30^2, 31, 32^2, 33^2, 36, 38, 41, 43, 45, 52, 59, 64, 75, 96, 132]
surviving nonsolo ucounts = 1[41]
ids = [528]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.67 = CTCGAAACAACTGGCC-1

using 40 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[40]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.71 = CTCGAAACAATAGAGT-1

using 10387 reads

====================================================================================

graph has 6233 edges initially, 326 edges after simplification

total ucounts = 480
nonsolo ucounts = 388[2^31, 3^26, 4^12, 5^21, 6^12, 7^15, 8^16, 9^10, 10^8, 11^12, 12^9, 13^9, 14^8, 15^11, 16^11, 17^5, 18^5, 19^6, 20^5, 21^6, 22^5, 23^4, 24^6, 25^4, 26, 27^4, 28^3, 29^4, 31^6, 32^3, 33^6, 34^3, 35, 36^2, 37, 38^5, 40, 41^4, 42^5, 43^4, 44^2, 45^3, 46, 47^2, 48^3, 49^2, 50^5, 51, 52^2, 59^2, 62^2, 63^2, 64, 65, 66^2, 68^3, 69, 70^2, 71^3, 72^2, 73, 76^2, 78^2, 79, 80, 81, 83, 85, 86, 87, 89, 92, 94^3, 96, 100^2, 101, 103, 106^2, 107, 108, 109^2, 110^2, 111, 114, 116, 120, 128, 132]
surviving nonsolo ucounts = 1[83]
ids = [154]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.72 = CTCGAAACAATCGGTT-1

using 99 reads

====================================================================================

graph has 76 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[99]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.73 = CTCGAAACAATGGAAT-1

using 33 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.74 = CTCGAAACACAAGTAA-1

using 75 reads

====================================================================================

graph has 66 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 4[4, 10, 17, 41]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.76 = CTCGAAACACATCCGG-1

using 101 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[101]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.78 = CTCGAAACACCAGGTC-1

using 225 reads

====================================================================================

graph has 146 edges initially, 2 edges after simplification

total ucounts = 17
nonsolo ucounts = 14[2^4, 3^2, 4^3, 7, 11, 12, 14, 152]
surviving nonsolo ucounts = 1[152]
ids = [1]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.79 = CTCGAAACACCAGTTA-1

using 11272 reads

====================================================================================

graph has 8412 edges initially, 236 edges after simplification

total ucounts = 956
nonsolo ucounts = 714[2^117, 3^75, 4^60, 5^47, 6^32, 7^32, 8^33, 9^23, 10^21, 11^15, 12^27, 13^24, 14^19, 15^22, 16^15, 17^17, 18^4, 19^16, 20^14, 21^3, 22^15, 23^9, 24^9, 25^10, 26^5, 27^5, 28^3, 29^7, 30^5, 31, 32^4, 33^2, 35^4, 38, 44^2, 45, 46, 47, 48, 49, 53, 57^2, 60, 67, 68, 78, 80, 107, 157, 3139]
surviving nonsolo ucounts = 5[4, 35, 53, 157, 3139]
ids = [912, 835, 323, 574, 23]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.80 = CTCGAAACACCATCCT-1

using 250 reads

====================================================================================

graph has 184 edges initially, 12 edges after simplification

total ucounts = 12
nonsolo ucounts = 11[3^2, 7, 13, 16^2, 17, 20, 39, 49, 66]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.81 = CTCGAAACACCGAAAG-1

using 54 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[54]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.82 = CTCGAAACACGACTCG-1

using 2122 reads

====================================================================================

graph has 2215 edges initially, 46 edges after simplification

total ucounts = 330
nonsolo ucounts = 207[2^26, 3^21, 4^28, 5^14, 6^15, 7^19, 8^9, 9^11, 10^5, 11^10, 12^4, 13^7, 14^3, 15^3, 16^6, 17^5, 18, 19^3, 20, 21^2, 22^2, 23^2, 25, 28, 29, 35, 36, 40, 51, 83, 85, 102]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.84 = CTCGAAACACGGCCAT-1

using 21 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 1[17]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.91 = CTCGAAACAGATCGGA-1

using 79 reads

====================================================================================

graph has 88 edges initially, 4 edges after simplification

total ucounts = 7
nonsolo ucounts = 4[2, 9, 17, 48]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.95 = CTCGAAACAGCTTCGG-1

using 57 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[22, 35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.98 = CTCGAAACAGTGACAG-1

using 29 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.101 = CTCGAAACATACCATG-1

using 141 reads

====================================================================================

graph has 88 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 5[5, 6, 14, 29, 86]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.103 = CTCGAAACATCCGGGT-1

using 50 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[50]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.106 = CTCGAAACATCTGGTA-1

using 66 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[66]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.108 = CTCGAAACATGACATC-1

using 119 reads

====================================================================================

graph has 76 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 5[5, 7, 11, 13, 81]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.109 = CTCGAAACATGCCTAA-1

using 63 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[63]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.110 = CTCGAAACATTCCTCG-1

using 48 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[48]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.111 = CTCGAAACATTTGCTT-1

using 38 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.112 = CTCGAAAGTAACGACG-1

using 49 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 1[46]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.115 = CTCGAAAGTATGAAAC-1

using 58 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 3[5, 11, 39]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.120 = CTCGAAAGTCATACTG-1

using 36 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.121 = CTCGAAAGTCCAAGTT-1

using 52 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[52]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.123 = CTCGAAAGTCGATTGT-1

using 41 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[41]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.126 = CTCGAAAGTGCAGACA-1

using 79 reads

====================================================================================

graph has 74 edges initially, 4 edges after simplification

total ucounts = 11
nonsolo ucounts = 6[2, 10, 12, 13, 16, 21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.129 = CTCGAAAGTGGCCCTA-1

using 17 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[17]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.132 = CTCGAAAGTGGTCTCG-1

using 59 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[59]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.133 = CTCGAAAGTGTCTGAT-1

using 87 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[87]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.134 = CTCGAAAGTGTTAAGA-1

using 3997 reads

====================================================================================

graph has 3094 edges initially, 138 edges after simplification

total ucounts = 369
nonsolo ucounts = 265[2^39, 3^14, 4^15, 5^13, 6^8, 7^14, 8^8, 9^10, 10^7, 11^14, 12^8, 13^6, 14^6, 15^6, 16^9, 17^5, 18^7, 19^3, 20^3, 21^9, 22^3, 23^5, 24^8, 25^4, 26^4, 27^3, 28^4, 29^3, 30^3, 31, 32, 34, 35^2, 36^2, 37, 38, 39, 40, 41, 44, 46, 48^2, 52, 55, 59, 64, 69, 76, 85, 88]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.136 = CTCGAAAGTGTTTGTG-1

using 61 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 1[56]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.137 = CTCGAAAGTTAAGATG-1

using 35 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.138 = CTCGAAAGTTACGGAG-1

using 1395 reads

====================================================================================

graph has 286 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[2, 1391]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.142 = CTCGAAAGTTCGTTGA-1

using 77 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[77]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.143 = CTCGAAAGTTGAGGTG-1

using 54 reads

====================================================================================

graph has 48 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 5[2, 3, 4, 9, 33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.144 = CTCGAAAGTTGGGACA-1

using 30 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[4, 26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.145 = CTCGAAAGTTGTCTTT-1

using 40 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[40]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.148 = CTCGAAATCAACCATG-1

using 2125 reads

====================================================================================

graph has 716 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 3[2, 5, 2114]
surviving nonsolo ucounts = 1[2114]
ids = [5]

====================================================================================

UMI info for barcode CTCGAAATCAACCATG-1 contig 1 = AGAGGCCCCA...
umi TACACGAGTC = 2115 reads: +403 validated

GOOD CONTIGS

TIG 1[bases=484]
0-29 ==> 0-29 on |622|TRBV4-1|5'UTR| [len=29] (mis=0)
29-373 ==> 0-344 on |623|TRBV4-1|L-REGION+V-REGION| [len=344] (mis=3)
370-382 ==> 0-12 on |535|TRBD1|D-REGION| [len=12] (mis=3)
383-432 ==> 0-49 on |546|TRBJ2-3|J-REGION| [len=49] (mis=0)
432-484 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 1 umis using 140 reads
cdr3 = CASSPYQGASTDTQYF at 356, score = 10 + 8
umis assigned: [5]
of which 1 are surviving nonsolos
reads assigned: 2079
start codons at 29, 122, 128, 151, 164, 179, 221, 237, 255, 286
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.149 = CTCGAAATCAAGGTAA-1

using 42 reads

====================================================================================

graph has 32 edges initially, 4 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[2, 18, 21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.153 = CTCGAAATCACTCCTG-1

using 72 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[72]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.156 = CTCGAAATCATCGCTC-1

using 22 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.157 = CTCGAAATCATGGTCA-1

using 661 reads

====================================================================================

graph has 248 edges initially, 6 edges after simplification

total ucounts = 11
nonsolo ucounts = 5[2^2, 30, 34, 587]
surviving nonsolo ucounts = 1[587]
ids = [4]

====================================================================================

UMI info for barcode CTCGAAATCATGGTCA-1 contig 1 = GCAGATCTCT...
umi ATGGGACTCG = 586 reads: +403 validated

GOOD CONTIGS

TIG 1[bases=466]
0-21 ==> 15-36 on |455|TRAV10|5'UTR| [len=36] (mis=0)
21-364 ==> 0-343 on |456|TRAV10|L-REGION+V-REGION| [len=343] (mis=1)
370-424 ==> 9-63 on |435|TRAJ48|J-REGION| [len=63] (mis=0)
424-466 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 1 umis using 46 reads
cdr3 = CVVSGWVGNEKLTF at 351, score = 6 + 10
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 582
start codons at 21, 79, 149, 231, 283, 365, 376
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.159 = CTCGAAATCCAGATCA-1

using 73 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[73]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.161 = CTCGAAATCCGAACGC-1

using 42 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[42]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.162 = CTCGAAATCCGCGGTA-1

using 50 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[2, 3, 44]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.163 = CTCGAAATCCTCCTAG-1

using 34 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.165 = CTCGAAATCGATCCCT-1

using 222 reads

====================================================================================

graph has 222 edges initially, 6 edges after simplification

total ucounts = 10
nonsolo ucounts = 7[4, 10, 14, 22, 41, 61, 67]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.170 = CTCGAAATCTAACGGT-1

using 27 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.172 = CTCGAAATCTAACTTC-1

using 35045 reads

====================================================================================

graph has 17592 edges initially, 646 edges after simplification

total ucounts = 1497
nonsolo ucounts = 1191[2^142, 3^94, 4^89, 5^64, 6^69, 7^39, 8^60, 9^50, 10^37, 11^43, 12^32, 13^25, 14^37, 15^35, 16^24, 17^23, 18^26, 19^26, 20^22, 21^15, 22^25, 23^15, 24^12, 25^15, 26^14, 27^11, 28^9, 29^8, 30^10, 31^7, 32^8, 33^13, 34^5, 35^2, 36^6, 37^6, 38^7, 39^9, 40^8, 41^7, 42^4, 43, 44^4, 45^2, 46^2, 47^6, 48^2, 50^3, 52^2, 53^3, 54^3, 62, 79, 90, 96, 99, 107, 763, 4270, 5938, 7959]
surviving nonsolo ucounts = 4[763, 4270, 5938, 7959]
ids = [577, 946, 750, 767]

====================================================================================

REJECT CONTIGS

TIG 1[bases=406]
3-49 ==> 0-46 on |545|TRBJ2-2P|J-REGION| [len=46] (mis=0)
49-153 ==> 0-104 on segment before TRBJ2-3 exon 1 [len=104] (mis=0)
202-304 ==> 0-102 on segment before TRBJ2-4 exon 1 [len=102] (mis=0)
354-406 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
umis assigned: [422, 577, 750, 767, 946]
of which 4 are surviving nonsolos
reads assigned: 18475
start codons at 63
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.175 = CTCGAAATCTCAACTT-1

using 78 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[78]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.177 = CTCGAAATCTCGCTTG-1

using 33 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.181 = CTCGAAATCTTATCTG-1

using 580 reads

====================================================================================

graph has 526 edges initially, 16 edges after simplification

total ucounts = 29
nonsolo ucounts = 23[2^2, 3^3, 4^3, 6, 8, 9, 10, 11, 14, 16, 21, 24, 27, 35, 53, 85, 115^2]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.182 = CTCGAAATCTTCAACT-1

using 84 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[84]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.184 = CTCGAAATCTTGTACT-1

using 42 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 3[4, 14, 20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.185 = CTCGAAATCTTTACGT-1

using 324 reads

====================================================================================

graph has 234 edges initially, 6 edges after simplification

total ucounts = 15
nonsolo ucounts = 12[4, 6^2, 12, 13, 14, 16, 26, 31, 55, 57, 81]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.190 = CTCGAGGAGACTGGGT-1

using 58 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[58]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.201 = CTCGAGGAGCGTAATA-1

using 14579 reads

====================================================================================

graph has 5094 edges initially, 100 edges after simplification

total ucounts = 270
nonsolo ucounts = 204[2^30, 3^25, 4^15, 5^8, 6^14, 7^13, 8^8, 9^10, 10^6, 11^8, 12^8, 13^7, 14^3, 15^9, 16^2, 17^5, 18, 21^6, 22^2, 24, 25, 26, 27, 30, 34, 35^2, 40, 43, 50, 51, 54, 58, 61, 62, 76, 94, 100, 388, 1879, 1911, 2810, 5219]
surviving nonsolo ucounts = 5[388, 1879, 1911, 2810, 5219]
ids = [47, 245, 98, 22, 224]

====================================================================================

UMI info for barcode CTCGAGGAGCGTAATA-1 contig 1 = TGGGGCATTT...
umi ACAGTCCTTT = 2824 reads: +400 validated
umi CCGATTGTCA = 1916 reads: +400 validated

GOOD CONTIGS

TIG 1[bases=457]
15-353 ==> 0-338 on |477|TRAV2|L-REGION+V-REGION| [len=338] (mis=0)
355-415 ==> 0-60 on |449|TRAJ8|J-REGION| [len=60] (mis=4)
415-457 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 2 umis using 360 reads
cdr3 = CAVEDRGTGFQKLVF at 339, score = 7 + 8
umis assigned: [22, 98]
of which 2 are surviving nonsolos
reads assigned: 4668
start codons at 15, 172, 209, 267, 274, 322
confident = true

REJECT CONTIGS

TIG 1[bases=370]
0-46 ==> 0-46 on |661|TRBV6-6|5'UTR| [len=46] (mis=0)
46-289 ==> 0-243 on |662|TRBV6-6|L-REGION+V-REGION| [len=344] (mis=1)
288-318 ==> 19-49 on |546|TRBJ2-3|J-REGION| [len=49] (mis=0)
318-370 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
umis assigned: [47, 224, 245]
of which 3 are surviving nonsolos
reads assigned: 6140
start codons at 4, 46, 104, 157, 181, 196, 223
confident = false
did not find CDR3
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.206 = CTCGAGGAGCTGAACG-1

using 55 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[5, 9, 40]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.210 = CTCGAGGAGCTTATCG-1

using 20174 reads

====================================================================================

graph has 10932 edges initially, 254 edges after simplification

total ucounts = 1061
nonsolo ucounts = 811[2^102, 3^100, 4^59, 5^56, 6^48, 7^50, 8^47, 9^29, 10^28, 11^31, 12^18, 13^19, 14^20, 15^14, 16^15, 17^18, 18^12, 19^8, 20^8, 21^9, 22^12, 23^8, 24^4, 25^8, 26^7, 27^6, 28^4, 29^4, 30^8, 31^5, 32^2, 33^3, 34^3, 35, 36, 39^2, 40^2, 41, 43, 44^2, 46^3, 47^2, 50^3, 52^2, 54, 60, 61^2, 62, 64, 67, 69^3, 70, 72^2, 82, 90, 93, 108^2, 116, 622, 874, 995, 1208, 1219, 1408, 4156]
surviving nonsolo ucounts = 7[108, 622, 874, 995, 1208, 1219, 4156]
ids = [889, 286, 921, 364, 680, 356, 631]

====================================================================================

UMI info for barcode CTCGAGGAGCTTATCG-1 contig 1 = CAGAAGAGGA...
umi ATTGGCACAG = 583 reads: +391 validated
umi CATTAGCCTT = 1001 reads: +391 validated
umi GCACCGTCAC = 3426 reads: -313X +1 -2XX +1 -3XX +4 -2XX +1 -6XX +1 -8XX +1 -5XX +43 invalidated
umi GTTAGCCATG = 3 reads: -391X invalidated
umi TCGTCCATGC = 80 reads: -389X +2 invalidated

UMI info for barcode CTCGAGGAGCTTATCG-1 contig 2 = GAGAAGCCTC...
umi AAGTTGTGCG = 502 reads: -376X +1 -1XX +2 -1XX +4 -3XX +1 -3XX +4 -4XX +1 -2XX invalidated
umi CATATTCTGT = 1232 reads: +403 validated
umi GGCAACTGTC = 1206 reads: +403 validated
umi TCTTTTTCCT = 877 reads: +403 validated

GOOD CONTIGS

TIG 1[bases=498]
0-55 ==> 57-112 on |618|TRBV30|5'UTR| [len=112] (mis=0)
55-390 ==> 0-335 on |619|TRBV30|L-REGION+V-REGION| [len=335] (mis=0)
396-446 ==> 0-50 on |540|TRBJ1-5|J-REGION| [len=50] (mis=4)
446-498 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 2 umis using 103 reads
cdr3 = CAWSVRTVNQPQHF at 376, score = 7 + 8
umis assigned: [286, 364, 631, 749, 889]
of which 4 are surviving nonsolos
reads assigned: 4976
start codons at 52, 55, 123, 422
confident = true

TIG 2[bases=562]
0-117 ==> 0-117 on |474|TRAV19|5'UTR| [len=117] (mis=1)
117-467 ==> 0-350 on |475|TRAV19|L-REGION+V-REGION| [len=350] (mis=4)
468-520 ==> 6-58 on |420|TRAJ34|J-REGION| [len=58] (mis=0)
520-562 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 3 umis using 135 reads
cdr3 = CALSHINTDKLIF at 450, score = 7 + 9
umis assigned: [49, 356, 680, 921]
of which 3 are surviving nonsolos
reads assigned: 3765
start codons at 111, 117, 174, 229, 250, 340, 349
confident = true
now this is a cell
paired!

TCACAAGTCGTGGACTCAGCAGTATACTTCTGTGCTCTGAGTCACATCAACACCGACAAGCTCATCTTTGGGACTGGGACCAGATTACAAGTCTTTCCAA <==> CTCCTTCTCAGTGACTCTGGCTTCTATCTCTGTGCCTGGAGTGTACGGACAGTTAATCAGCCCCAGCATTTTGGTGATGGGACTCGACTCTCCATCCTAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.213 = CTCGAGGAGGAATGGA-1

using 47 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[47]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.216 = CTCGAGGAGGATATAC-1

using 173 reads

====================================================================================

graph has 86 edges initially, 6 edges after simplification

total ucounts = 9
nonsolo ucounts = 8[2, 3, 6^2, 7, 26, 42, 80]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.220 = CTCGAGGAGGCTCAGA-1

using 66 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 3[16, 19, 28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.221 = CTCGAGGAGGGAAACA-1

using 23 reads

====================================================================================

graph has 4 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.224 = CTCGAGGAGGTGCTAG-1

using 35 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.225 = CTCGAGGAGTACGACG-1

using 137 reads

====================================================================================

graph has 54 edges initially, 4 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[2, 49, 84]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.227 = CTCGAGGAGTCCCACG-1

using 18 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.231 = CTCGAGGAGTGTTGAA-1

using 73 reads

====================================================================================

graph has 110 edges initially, 4 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[8, 9, 15, 39]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.239 = CTCGAGGCAACAACCT-1

using 57 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 4[2, 7, 11, 32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.246 = CTCGAGGCAATAACGA-1

using 73 reads

====================================================================================

graph has 86 edges initially, 4 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[17, 56]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.252 = CTCGAGGCACAGCGTC-1

using 111 reads

====================================================================================

graph has 96 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 7[2^2, 4, 6, 12, 17, 64]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.256 = CTCGAGGCACGACTCG-1

using 38 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.258 = CTCGAGGCACGGTGTC-1

using 86 reads

====================================================================================

graph has 68 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[86]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.259 = CTCGAGGCACGTAAGG-1

using 42 reads

====================================================================================

graph has 58 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 5[2, 3^2, 6, 28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.260 = CTCGAGGCACTCGACG-1

using 94 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[93]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.261 = CTCGAGGCACTGTTAG-1

using 30 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.264 = CTCGAGGCAGATCTGT-1

using 27 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.268 = CTCGAGGCAGCTTCGG-1

using 27 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[3, 22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.271 = CTCGAGGCATACTACG-1

using 21 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.273 = CTCGAGGCATAGTAAG-1

using 79 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[79]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.275 = CTCGAGGCATCCAACA-1

using 22 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.279 = CTCGAGGCATCTGGTA-1

using 33 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.280 = CTCGAGGCATGCATGT-1

using 79 reads

====================================================================================

graph has 68 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[79]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.284 = CTCGAGGCATTAGGCT-1

using 13991 reads

====================================================================================

graph has 10803 edges initially, 288 edges after simplification

total ucounts = 1388
nonsolo ucounts = 971[2^197, 3^118, 4^94, 5^61, 6^55, 7^48, 8^41, 9^27, 10^30, 11^32, 12^31, 13^29, 14^20, 15^19, 16^13, 17^16, 18^18, 19^11, 20^6, 21^5, 22^10, 23^7, 24^6, 25^4, 26^8, 27^7, 28^5, 29^5, 30^3, 31^5, 32^2, 33, 34^3, 36^2, 37^4, 38, 39, 40^3, 41^2, 42, 46, 48, 51^3, 52, 53, 54, 55, 56, 58, 59, 64, 67, 90, 100, 104, 118, 828, 3407]
surviving nonsolo ucounts = 1[3407]
ids = [140]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.288 = CTCGAGGGTACCAGTT-1

using 272 reads

====================================================================================

graph has 150 edges initially, 12 edges after simplification

total ucounts = 12
nonsolo ucounts = 12[2, 6, 7, 11, 14, 17, 26, 28, 30, 40, 45, 46]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.291 = CTCGAGGGTAGCTAAA-1

using 22 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.295 = CTCGAGGGTCAAAGAT-1

using 63 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[63]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.297 = CTCGAGGGTCAGTGGA-1

using 35 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 4[2, 3, 4, 25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.300 = CTCGAGGGTCGCGAAA-1

using 138 reads

====================================================================================

graph has 76 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[138]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.302 = CTCGAGGGTGAAAGAG-1

using 15 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.304 = CTCGAGGGTGAGTATA-1

using 88 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[88]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.312 = CTCGAGGGTGTGACCC-1

using 63 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[63]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.316 = CTCGAGGGTTAAAGAC-1

using 92 reads

====================================================================================

graph has 118 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 6[3, 4, 7^3, 62]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.317 = CTCGAGGGTTAAAGTG-1

using 47 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 5[2^2, 3, 12, 27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.319 = CTCGAGGGTTAGATGA-1

using 89 reads

====================================================================================

graph has 72 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 6[2, 5, 7, 8, 17, 48]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.324 = CTCGAGGGTTTGTGTG-1

using 201 reads

====================================================================================

graph has 70 edges initially, 6 edges after simplification

total ucounts = 8
nonsolo ucounts = 6[2, 11, 14, 27, 51, 94]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.326 = CTCGAGGTCAACACGT-1

using 23 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.328 = CTCGAGGTCACAGGCC-1

using 18 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[17]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.331 = CTCGAGGTCACGCATA-1

using 80000 reads

====================================================================================

graph has 42962 edges initially, 1555 edges after simplification

total ucounts = 4534
nonsolo ucounts = 3823[2^457, 3^360, 4^266, 5^210, 6^165, 7^112, 8^112, 9^106, 10^84, 11^87, 12^69, 13^71, 14^68, 15^63, 16^54, 17^54, 18^51, 19^48, 20^41, 21^31, 22^33, 23^35, 24^40, 25^39, 26^39, 27^37, 28^31, 29^34, 30^43, 31^33, 32^28, 33^35, 34^26, 35^32, 36^34, 37^35, 38^20, 39^22, 40^29, 41^27, 42^28, 43^14, 44^29, 45^19, 46^19, 47^23, 48^21, 49^17, 50^23, 51^22, 52^25, 53^15, 54^23, 55^18, 56^14, 57^13, 58^9, 59^18, 60^19, 61^15, 62^15, 63^20, 64^18, 65^8, 66^18, 67^9, 68^8, 69^14, 70^12, 71^12, 72^7, 73^8, 74^6, 75^6, 76^12, 77^13, 78^5, 79^7, 80^8, 81^2, 82^9, 83^5, 84^4, 85^2, 86^3, 87^3, 89^6, 90^4, 91^2, 93^3, 94^2, 96, 97^4, 98, 99^5, 100^2, 101, 102, 104^4, 106, 107, 108, 109, 110, 127, 130, 547]
surviving nonsolo ucounts = 4[3, 59, 110, 547]
ids = [336, 4194, 777, 3609]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.334 = CTCGAGGTCAGCTCTC-1

using 30 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[3, 27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.336 = CTCGAGGTCAGTACGT-1

using 524 reads

====================================================================================

graph has 408 edges initially, 12 edges after simplification

total ucounts = 23
nonsolo ucounts = 21[2, 3^4, 4, 5, 7, 8, 12, 19, 26^3, 29, 30, 31, 33, 49, 88, 115]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.341 = CTCGAGGTCATGGTCA-1

using 75 reads

====================================================================================

graph has 68 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[75]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.352 = CTCGAGGTCCTGCAGG-1

using 27 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.353 = CTCGAGGTCGAACTGT-1

using 32 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.355 = CTCGAGGTCGAATGCT-1

using 39 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[39]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.356 = CTCGAGGTCGACCAGC-1

using 106 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[106]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.360 = CTCGAGGTCGCCATAA-1

using 22 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.361 = CTCGAGGTCGCGCCAA-1

using 84 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[84]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.364 = CTCGAGGTCGTCGTTC-1

using 51 reads

====================================================================================

graph has 2 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[51]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.368 = CTCGAGGTCTAACCGA-1

using 46 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[46]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.369 = CTCGAGGTCTAACTGG-1

using 58 reads

====================================================================================

graph has 124 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 3[4^2, 47]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.370 = CTCGAGGTCTAGAGTC-1

using 374 reads

====================================================================================

graph has 280 edges initially, 10 edges after simplification

total ucounts = 16
nonsolo ucounts = 12[2, 3^2, 5, 22, 23, 29, 30, 35, 55, 58, 105]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.372 = CTCGAGGTCTCCAGGG-1

using 134 reads

====================================================================================

graph has 76 edges initially, 6 edges after simplification

total ucounts = 6
nonsolo ucounts = 5[16, 20, 24, 31, 42]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.383 = CTCGAGGTCTGTCAAG-1

using 50 reads

====================================================================================

graph has 66 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 7[3^2, 4^2, 5, 8, 21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.384 = CTCGAGGTCTTATCTG-1

using 16 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[3, 13]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.387 = CTCGAGGTCTTGAGGT-1

using 70 reads

====================================================================================

graph has 56 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 5[2^2, 7, 15, 43]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.388 = CTCGAGGTCTTGCAAG-1

using 891 reads

====================================================================================

graph has 354 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[891]
surviving nonsolo ucounts = 1[891]
ids = [0]

====================================================================================

UMI info for barcode CTCGAGGTCTTGCAAG-1 contig 1 = ATCAATGCAC...
umi GAAACCGCTA = 896 reads: +397 validated

GOOD CONTIGS

TIG 1[bases=495]
0-46 ==> 0-46 on |661|TRBV6-6|5'UTR| [len=46] (mis=0)
46-390 ==> 0-344 on |662|TRBV6-6|L-REGION+V-REGION| [len=344] (mis=4)
393-443 ==> 0-50 on |543|TRBJ2-1|J-REGION| [len=50] (mis=3)
443-495 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 1 umis using 54 reads
cdr3 = CASSFTGVYNEQFF at 373, score = 9 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 876
start codons at 4, 46, 104, 157, 181, 196, 223, 287, 401
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.389 = CTCGAGGTCTTGTTTG-1

using 42 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 4[7, 8, 10, 14]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.390 = CTCGAGGTCTTTCCTC-1

using 68 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[67]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.391 = CTCGGAGAGAACAATC-1

using 146 reads

====================================================================================

graph has 166 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 7[3, 4, 8, 13, 22, 30, 65]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.393 = CTCGGAGAGAAGGACA-1

using 53 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[53]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.395 = CTCGGAGAGACATAAC-1

using 303 reads

====================================================================================

graph has 128 edges initially, 14 edges after simplification

total ucounts = 16
nonsolo ucounts = 13[2, 3, 4^2, 5, 8, 11, 14, 24, 26, 40, 42, 117]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.397 = CTCGGAGAGACTCGGA-1

using 80 reads

====================================================================================

graph has 48 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 5[2, 5, 10, 11, 49]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.400 = CTCGGAGAGAGTAAGG-1

using 1669 reads

====================================================================================

graph has 796 edges initially, 12 edges after simplification

total ucounts = 36
nonsolo ucounts = 25[2^2, 3, 4^2, 5, 6^3, 7, 9^2, 10, 17, 18, 20, 22, 30, 42, 47, 49, 51, 66, 70, 1153]
surviving nonsolo ucounts = 1[1153]
ids = [28]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.404 = CTCGGAGAGATGAGAG-1

using 58 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[2, 55]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.408 = CTCGGAGAGCCGATTT-1

using 55 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[55]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.412 = CTCGGAGAGGAGTTTA-1

using 78 reads

====================================================================================

graph has 68 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 6[2, 5, 7, 16, 21, 26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.414 = CTCGGAGAGGGATGGG-1

using 46 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[46]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.417 = CTCGGAGAGTCCGGTC-1

using 99 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[99]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.419 = CTCGGAGAGTGGGTTG-1

using 43 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[43]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.425 = CTCGGAGCAACACCTA-1

using 42 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[42]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.426 = CTCGGAGCAACACGCC-1

using 2936 reads

====================================================================================

graph has 2020 edges initially, 44 edges after simplification

total ucounts = 265
nonsolo ucounts = 172[2^44, 3^17, 4^22, 5^9, 6^11, 7^17, 8^4, 9^6, 10^3, 11^4, 12^6, 14^2, 15^3, 16, 17^3, 18^2, 21^2, 22, 24^2, 28, 29, 31, 45, 47, 51, 54, 63, 74, 75, 87, 405, 877]
surviving nonsolo ucounts = 2[405, 877]
ids = [85, 180]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.429 = CTCGGAGCAAGGGTCA-1

using 372 reads

====================================================================================

graph has 148 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[2, 4, 14, 350]
surviving nonsolo ucounts = 1[350]
ids = [4]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.449 = CTCGGAGCAGCCTATA-1

using 7295 reads

====================================================================================

graph has 2778 edges initially, 68 edges after simplification

total ucounts = 299
nonsolo ucounts = 199[2^33, 3^27, 4^17, 5^17, 6^10, 7^13, 8^15, 9^7, 10^6, 11^3, 12^3, 13^2, 14^4, 15^5, 16^3, 17^4, 18^2, 19^2, 20^2, 21^2, 22, 24, 25, 28, 30, 31^2, 36, 37^2, 42, 43, 48, 56, 63, 64, 70, 71, 74, 94, 140, 4937]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.452 = CTCGGAGCAGGCAGTA-1

using 31 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.455 = CTCGGAGCATCAGTAC-1

using 39 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[39]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.459 = CTCGGAGCATGTTGAC-1

using 39 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 1[36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.472 = CTCGGAGGTACGAAAT-1

using 89 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[89]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.475 = CTCGGAGGTAGGACAC-1

using 78 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[78]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.480 = CTCGGAGGTCAAACTC-1

using 37 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.483 = CTCGGAGGTCAACTGT-1

using 11682 reads

====================================================================================

graph has 6394 edges initially, 212 edges after simplification

total ucounts = 653
nonsolo ucounts = 531[2^63, 3^29, 4^30, 5^29, 6^28, 7^19, 8^23, 9^24, 10^16, 11^6, 12^13, 13^13, 14^12, 15^13, 16^7, 17^8, 18^5, 19^3, 20^7, 21^4, 22^8, 23^5, 24^4, 25^3, 26^3, 27^5, 28^4, 29^7, 30^4, 31^4, 32^7, 33^5, 34^4, 35^3, 36^5, 37^4, 38, 39^2, 40^4, 41^3, 43^4, 44, 45^2, 46^2, 48, 49^2, 50^3, 51^3, 52^3, 53^8, 54, 55^2, 56^4, 57, 58^2, 59^2, 61, 62^4, 63, 64, 65, 67^2, 68^3, 69^3, 70, 71, 72^2, 74^3, 75^2, 76^2, 77^2, 78, 79, 81, 82^2, 85^4, 86^3, 87^2, 88, 90, 92, 94, 96, 98, 99, 102, 104, 105]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.484 = CTCGGAGGTCACACGC-1

using 3831 reads

====================================================================================

graph has 3066 edges initially, 86 edges after simplification

total ucounts = 455
nonsolo ucounts = 316[2^67, 3^45, 4^32, 5^18, 6^18, 7^15, 8^7, 9^7, 10^8, 11^15, 12^5, 13^5, 14^4, 15^7, 16^4, 17^4, 18^4, 19^7, 20, 21^3, 22^4, 23^2, 24, 25^2, 27^2, 28^2, 29, 30, 34, 35^2, 36^2, 38^2, 39^2, 40, 43, 45, 47^2, 48, 55, 68^2, 69, 71, 80, 82, 83, 180, 189]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.485 = CTCGGAGGTCACTTCC-1

using 425 reads

====================================================================================

graph has 380 edges initially, 12 edges after simplification

total ucounts = 28
nonsolo ucounts = 23[2^3, 3^3, 4^3, 5, 6^2, 9, 11, 13^2, 23, 27, 28, 48, 52, 63, 89]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.488 = CTCGGAGGTCGAGTTT-1

using 13 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[13]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.489 = CTCGGAGGTCGCGAAA-1

using 91 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[91]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.491 = CTCGGAGGTCGCTTTC-1

using 8972 reads

====================================================================================

graph has 6886 edges initially, 126 edges after simplification

total ucounts = 917
nonsolo ucounts = 647[2^131, 3^101, 4^51, 5^75, 6^48, 7^21, 8^28, 9^24, 10^18, 11^15, 12^10, 13^12, 14^18, 15^16, 16^7, 17^6, 18^6, 19^4, 20^3, 21^10, 22^6, 23^3, 24^4, 25, 27, 28, 29, 30, 32, 33^2, 35^2, 36^2, 38, 39, 40, 41, 42, 47, 48^2, 50, 54, 56, 59, 68, 69, 113, 174, 463, 2777]
surviving nonsolo ucounts = 3[174, 463, 2777]
ids = [474, 221, 590]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.493 = CTCGGAGGTCTGGAGA-1

using 45 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 5[2^3, 3, 36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.497 = CTCGGAGGTGATGTGG-1

using 19 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.500 = CTCGGAGGTGCATCTA-1

using 21 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.502 = CTCGGAGGTGTCTGAT-1

using 80 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[80]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.504 = CTCGGAGGTGTTCGAT-1

using 93 reads

====================================================================================

graph has 64 edges initially, 4 edges after simplification

total ucounts = 8
nonsolo ucounts = 5[2, 5, 9, 12, 62]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.506 = CTCGGAGGTTAAAGTG-1

using 44 reads

====================================================================================

graph has 54 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[2^2, 17, 21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.512 = CTCGGAGGTTGAGTTC-1

using 327 reads

====================================================================================

graph has 128 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[327]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.518 = CTCGGAGTCACAGTAC-1

using 124 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[124]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.519 = CTCGGAGTCACATAGC-1

using 8688 reads

====================================================================================

graph has 8082 edges initially, 204 edges after simplification

total ucounts = 1087
nonsolo ucounts = 811[2^139, 3^99, 4^67, 5^58, 6^43, 7^47, 8^33, 9^40, 10^29, 11^27, 12^28, 13^24, 14^21, 15^13, 16^14, 17^14, 18^7, 19^8, 20^6, 21^11, 22^8, 23^5, 24^6, 25^4, 26^4, 27^5, 28^7, 29^2, 30^3, 31^2, 32^2, 33, 34, 35, 37, 38, 40^2, 41^2, 42^2, 44^3, 46, 47, 48, 51, 52^2, 54^2, 55, 57^2, 62, 65, 66, 67, 77, 88, 91, 102, 103, 160]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.522 = CTCGGAGTCACGCGGT-1

using 2884 reads

====================================================================================

graph has 2374 edges initially, 40 edges after simplification

total ucounts = 367
nonsolo ucounts = 239[2^45, 3^23, 4^21, 5^15, 6^23, 7^12, 8^11, 9^8, 10^9, 11^7, 12^5, 13^6, 14^6, 15^3, 16^3, 17^3, 18, 19^3, 20, 21^3, 22^3, 24^2, 25, 26^2, 28^2, 29, 30^5, 33, 36, 37^2, 38^2, 41, 43, 46, 52^2, 67, 76, 116, 227]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.525 = CTCGGAGTCAGGCAAG-1

using 7764 reads

====================================================================================

graph has 4634 edges initially, 114 edges after simplification

total ucounts = 456
nonsolo ucounts = 314[2^71, 3^45, 4^33, 5^13, 6^18, 7^13, 8^16, 9^8, 10^11, 11^7, 12^5, 13^7, 14^4, 15^5, 16^6, 17^3, 18^4, 19, 20^3, 21^2, 22, 23^3, 24, 27^2, 28, 29^3, 30^2, 32^2, 33, 37, 39, 45, 47, 48, 50, 57, 58^2, 59^2, 60, 68, 72, 76, 87, 106, 113, 153^2, 487, 1364, 2203]
surviving nonsolo ucounts = 4[153, 487, 1364, 2203]
ids = [313, 449, 150, 82]

====================================================================================

UMI info for barcode CTCGGAGTCAGGCAAG-1 contig 1 = GAGGTGCAGT...
umi ATCTACCGGG = 2203 reads: +388 validated
umi CTTATGATGC = 5 reads: -387X +1 invalidated
umi GTCGTGTATT = 151 reads: +388 validated

UMI info for barcode CTCGGAGTCAGGCAAG-1 contig 2 = CCCACATGAA...
umi CCAGATGACA = 1370 reads: +391 validated
umi TTTGCAGCGC = 486 reads: +391 validated

GOOD CONTIGS

TIG 1[bases=470]
30-373 ==> 0-343 on |639|TRBV5-5|L-REGION+V-REGION| [len=343] (mis=4)
370-418 ==> 0-48 on |536|TRBJ1-1|J-REGION| [len=48] (mis=4)
junction support: 2 umis using 181 reads
cdr3 = CASSREAEAFF at 357, score = 8 + 8
umis assigned: [82, 221, 313]
of which 2 are surviving nonsolos
reads assigned: 2307
start codons at 30, 152, 235, 316
confident = true

TIG 2[bases=517]
0-84 ==> 0-84 on |453|TRAV1-2|5'UTR| [len=84] (mis=0)
84-402 ==> 0-318 on |454|TRAV1-2|L-REGION+V-REGION| [len=318] (mis=1)
409-475 ==> 0-66 on |418|TRAJ32|J-REGION| [len=66] (mis=4)
475-517 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 2 umis using 96 reads
cdr3 = CAVRAGYGGATNKLIF at 396, score = 7 + 8
umis assigned: [150, 449]
of which 2 are surviving nonsolos
reads assigned: 1810
start codons at 5, 33, 51, 84, 106, 114, 120, 162, 247, 280, 289, 372, 415
confident = true
now this is a cell
paired!

AAAGACTCTGCCTCTTACCTCTGTGCTGTGAGAGCGGGGTATGGTGGTGCTACAAACAAGCTCATCTTTGGAACTGGCACTCTGCTTGCTGTCCAGCCAA <==> GTGAACGCCTTGTTGCTGGGGGACTCGGCCCTGTATCTCTGTGCCAGCAGTCGGGAGGCTGAAGCTTTCTTTGGACAAGGCACCAGACTCACAGTTGTAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.526 = CTCGGAGTCATAACCG-1

using 7481 reads

====================================================================================

graph has 4810 edges initially, 120 edges after simplification

total ucounts = 441
nonsolo ucounts = 318[2^58, 3^32, 4^25, 5^19, 6^17, 7^13, 8^11, 9^8, 10^9, 11^13, 12^9, 13^17, 14^12, 15^6, 16^7, 17^6, 18^4, 19^6, 20^9, 21^2, 22^3, 23^3, 24^4, 25^2, 26^2, 27^2, 28, 29^2, 30, 33, 34^2, 35, 36^2, 52, 53, 55, 60, 73, 648, 662, 1177, 1665]
surviving nonsolo ucounts = 5[24, 648, 662, 1177, 1665]
ids = [89, 413, 337, 268, 43]

====================================================================================

UMI info for barcode CTCGGAGTCATAACCG-1 contig 1 = ATATGTAAAA...
umi ACCCGACCAT = 1668 reads: +412 validated
umi GCGGCAAATG = 1180 reads: +412 validated

UMI info for barcode CTCGGAGTCATAACCG-1 contig 2 = TGGGGGCTGT...
umi TACACGCCGT = 655 reads: +403 validated

GOOD CONTIGS

TIG 1[bases=565]
0-111 ==> 0-111 on |494|TRAV29/DV5|5'UTR| [len=111] (mis=0)
111-461 ==> 0-350 on |495|TRAV29/DV5|L-REGION+V-REGION| [len=358] (mis=0)
461-523 ==> 0-62 on |423|TRAJ37|J-REGION| [len=62] (mis=5)
523-565 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 2 umis using 137 reads
cdr3 = CALSGNTGKLIF at 456, score = 9 + 8
umis assigned: [43, 268]
of which 2 are surviving nonsolos
reads assigned: 2794
start codons at 2, 9, 30, 111, 117, 184, 187, 273, 290, 358, 364
confident = true

TIG 2[bases=654]
62-404 ==> 0-342 on |733|TRBV11-2|L-REGION+V-REGION| [len=342] (mis=1)
412-424 ==> 0-12 on |535|TRBD1|D-REGION| [len=12] (mis=3)
421-465 ==> 6-50 on |540|TRBJ1-5|J-REGION| [len=50] (mis=0)
junction support: 1 umis using 86 reads
cdr3 = CASSLQPGQGQPQHF at 392, score = 8 + 8
umis assigned: [337]
of which 1 are surviving nonsolos
reads assigned: 647
start codons at 62, 204, 285, 441, 466, 510, 571
confident = true

REJECT CONTIGS

TIG 1[bases=492]
0-47 ==> 0-47 on |616|TRBV3-1|5'UTR| [len=47] (mis=2)
47-391 ==> 0-344 on |617|TRBV3-1|L-REGION+V-REGION| [len=344] (mis=31)
390-440 ==> 0-50 on |543|TRBJ2-1|J-REGION| [len=50] (mis=5)
440-492 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
umis assigned: [89, 306, 413]
of which 2 are surviving nonsolos
reads assigned: 608
start codons at 47, 69, 146, 169, 192, 197, 239, 273, 398
confident = false
did not find CDR3
now this is a cell
paired!

CCCTCCCAGCCTGGAGACTCTGCAGTGTACTTCTGTGCCCTGTCTGGCAACACAGGCAAACTAATCTTTGGGCAAGGGACAACTTTACAAGTAAAACCAG <==> AAGCTTGAGGACTCGGCCGTGTATCTCTGTGCCAGCAGCTTGCAACCGGGACAGGGTCAGCCCCAGCATTTTGGTGATGGGACTCGACTCTCCATCCTAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.527 = CTCGGAGTCATAGCAC-1

using 24 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.529 = CTCGGAGTCATGTCTT-1

using 39 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[39]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.538 = CTCGGAGTCCGTAGTA-1

using 11997 reads

====================================================================================

graph has 4346 edges initially, 78 edges after simplification

total ucounts = 319
nonsolo ucounts = 206[2^35, 3^26, 4^17, 5^19, 6^7, 7^11, 8^7, 9^10, 10^5, 11^11, 12^5, 13^7, 14^3, 15, 16^5, 17^5, 19^4, 20, 23^2, 24, 25, 27, 28^2, 29, 30, 31, 33, 34, 37^2, 40^2, 44, 59, 67, 69, 158, 363, 427, 484, 683, 939, 6886]
surviving nonsolo ucounts = 7[158, 363, 427, 484, 683, 939, 6886]
ids = [251, 255, 158, 279, 58, 143, 167]

====================================================================================

UMI info for barcode CTCGGAGTCCGTAGTA-1 contig 1 = GATGTCTGTG...
umi CTCATATTCT = 938 reads: +397 validated
umi CTCATGATTA = 2 reads: -383 +1 -10XX +2 -1XX invalidated
umi CTTGTTTCCG = 6932 reads: -138 +1 -3XX +255 invalidated
umi TCACATCGTC = 160 reads: +277 -1 +119 non-validated
umi TCTTCACCCG = 486 reads: +367 -13 +17 non-validated

GOOD CONTIGS

TIG 1[bases=647]
0-208 ==> 17-225 on |520|TRAV8-2|5'UTR| [len=225] (mis=2)
208-549 ==> 0-341 on |521|TRAV8-2|L-REGION+V-REGION| [len=341] (mis=2)
547-605 ==> 0-58 on |420|TRAJ34|J-REGION| [len=58] (mis=5)
605-647 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 2 umis using 580 reads
cdr3 = CVVSDRNTDKLIF at 535, score = 7 + 9
umis assigned: [143, 144, 167, 251, 279]
of which 4 are surviving nonsolos
reads assigned: 8364
start codons at 1, 208, 371, 511
confident = true
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.546 = CTCGGAGTCGTTGCCT-1

using 1045 reads

====================================================================================

graph has 327 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[1044]
surviving nonsolo ucounts = 1[1044]
ids = [1]

====================================================================================

UMI info for barcode CTCGGAGTCGTTGCCT-1 contig 1 = GCACAGATAC...
umi CGGGTCTTAT = 1043 reads: +397 validated

GOOD CONTIGS

TIG 1[bases=489]
0-40 ==> 26-66 on |657|TRBV6-5|5'UTR| [len=66] (mis=0)
40-384 ==> 0-344 on |658|TRBV6-5|L-REGION+V-REGION| [len=344] (mis=5)
389-437 ==> 0-48 on |536|TRBJ1-1|J-REGION| [len=48] (mis=4)
437-489 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 1 umis using 77 reads
cdr3 = CASSPDRGVTEAFF at 367, score = 9 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 1031
start codons at 26, 40, 98, 151, 175, 182, 190, 217, 281, 290
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.547 = CTCGGAGTCTACGAGT-1

using 171 reads

====================================================================================

graph has 138 edges initially, 2 edges after simplification

total ucounts = 12
nonsolo ucounts = 11[2, 3, 4, 6, 7, 10, 11, 14, 25, 30, 58]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.549 = CTCGGAGTCTATCCTA-1

using 219 reads

====================================================================================

graph has 156 edges initially, 6 edges after simplification

total ucounts = 14
nonsolo ucounts = 10[3, 6, 9, 11, 14, 18, 27, 34, 39, 54]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.554 = CTCGGAGTCTCTAGGA-1

using 69 reads

====================================================================================

graph has 78 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 8[2, 3^3, 6, 13, 16, 21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.558 = CTCGGAGTCTGTCTAT-1

using 65 reads

====================================================================================

graph has 58 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[20, 45]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.560 = CTCGGGAAGAAGGGTA-1

using 198 reads

====================================================================================

graph has 100 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[198]
surviving nonsolo ucounts = 1[198]
ids = [0]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.570 = CTCGGGAAGCCACCTG-1

using 27 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.574 = CTCGGGAAGCGTGAGT-1

using 1694 reads

====================================================================================

graph has 562 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[1693]
surviving nonsolo ucounts = 1[1693]
ids = [0]

====================================================================================

UMI info for barcode CTCGGGAAGCGTGAGT-1 contig 1 = GGGGGGAGCC...
umi ACATACTCCC = 1680 reads: +397 validated

GOOD CONTIGS

TIG 1[bases=492]
0-43 ==> 0-43 on |610|TRBV27|5'UTR| [len=43] (mis=4)
43-387 ==> 0-344 on |611|TRBV27|L-REGION+V-REGION| [len=344] (mis=6)
381-393 ==> 0-12 on |535|TRBD1|D-REGION| [len=12] (mis=3)
390-440 ==> 0-50 on |547|TRBJ2-4|J-REGION| [len=50] (mis=4)
440-492 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 1 umis using 81 reads
cdr3 = CASRRQGVENIQYF at 370, score = 7 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 1648
start codons at 35, 43, 65, 178, 185, 193, 247, 251, 275
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.575 = CTCGGGAAGCTACCGC-1

using 98 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[98]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.589 = CTCGGGAAGTAGCGGT-1

using 78 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[77]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.594 = CTCGGGACAAAGAATC-1

using 49 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[49]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.595 = CTCGGGACAACGCACC-1

using 32 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[3, 28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.600 = CTCGGGACAAGTAGTA-1

using 26 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.601 = CTCGGGACAATCACAC-1

using 105 reads

====================================================================================

graph has 106 edges initially, 4 edges after simplification

total ucounts = 8
nonsolo ucounts = 8[2, 3^2, 4^2, 6, 35, 48]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.608 = CTCGGGACACCAACCG-1

using 20 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.611 = CTCGGGACACCGATAT-1

using 308 reads

====================================================================================

graph has 296 edges initially, 12 edges after simplification

total ucounts = 17
nonsolo ucounts = 15[2, 3^3, 4, 5, 11, 13, 16, 27, 33^2, 43, 53, 57]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.612 = CTCGGGACACGAAGCA-1

using 80000 reads

====================================================================================

graph has 17431 edges initially, 102 edges after simplification

total ucounts = 426
nonsolo ucounts = 262[2^54, 3^36, 4^31, 5^19, 6^11, 7^13, 8^10, 9^9, 10^3, 11^3, 12^5, 13^2, 14^4, 15, 16, 18^2, 19^2, 20^2, 21, 23, 26, 27, 28, 30^2, 32, 35, 38, 39, 47, 52, 55, 74, 78, 84, 166, 173, 186, 223, 303, 358, 385, 472, 835, 979, 1021, 1031, 1112, 1160, 1212, 1218, 1232, 1400, 1413, 1506, 1761, 1778, 2426, 2589, 3108, 3172, 3324, 3512, 3572, 3852, 4023, 4050, 4135, 4138, 4152, 5458, 6579]
surviving nonsolo ucounts = 31[303, 358, 472, 835, 979, 1021, 1031, 1112, 1160, 1212, 1218, 1232, 1400, 1413, 1506, 1761, 1778, 2589, 3108, 3172, 3324, 3512, 3572, 3852, 4023, 4050, 4135, 4138, 4152, 5458, 6579]
ids = [17, 37, 304, 357, 188, 165, 73, 421, 99, 57, ...]

====================================================================================

UMI info for barcode CTCGGGACACGAAGCA-1 contig 1 = ATGAGATCCT...
umi AGCCTCCCTT = 1203 reads: +394 validated
umi AGCTAGTGGC = 1785 reads: +394 validated
umi AGTTCATACC = 1045 reads: +394 validated
umi ATGTGTAGGG = 1129 reads: +394 validated
umi ATTCTTTGGA = 1423 reads: +394 validated
umi ATTGATCCCG = 1225 reads: +394 validated
umi CCCTGCACCC = 1522 reads: +11 -1XX +382 invalidated
umi CCTGAACTTC = 1027 reads: +394 validated
umi CTCCTTTAAG = 1237 reads: +394 validated
umi CTCTGATCGC = 3163 reads: -222X +1 -2XX +169 invalidated
umi GCCTCCACCG = 1805 reads: +12 -9XX +1 -4XX +1 -14XX +1 -5XX +347 invalidated
umi GGCCTCCGGT = 1406 reads: +394 validated
umi TACAATCCGC = 3355 reads: -357 +4 -2XX +2 -1XX +2 -1XX +5 -1XX +2 -1XX +5 -1XX +2 -1XX +5 -1XX +1 invalidated

GOOD CONTIGS

TIG 1[bases=470]
0-24 ==> 0-24 on |558|TRBV10-3|5'UTR| [len=24] (mis=0)
24-368 ==> 0-344 on |560|TRBV10-3|L-REGION+V-REGION| [len=344] (mis=5)
368-418 ==> 0-50 on |543|TRBJ2-1|J-REGION| [len=50] (mis=2)
418-470 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 12 umis using 1215 reads
cdr3 = CAISRDSFNEQFF at 351, score = 10 + 8
umis assigned: [57, 61, 73, 99, 105, 107, 151, 165, 208, 213] and 3 others
of which 13 are surviving nonsolos
reads assigned: 20877
start codons at 0, 24, 46, 78, 82, 146, 174, 202, 229, 265, 376
confident = true

REJECT CONTIGS

TIG 1[bases=545]
1-340 ==> 1512-1851 on segment before TRAJ39 exon 1 [len=1851] (mis=0)
444-503 ==> 0-59 on |412|TRAJ27|J-REGION| [len=59] (mis=0)
503-545 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
umis assigned: [188]
of which 1 are surviving nonsolos
reads assigned: 967
start codons at 141, 349, 360, 390, 452, 476
confident = false
did not find CDR3

TIG 2[bases=313]
5-99 ==> 5809-5903 on segment before TRAV8-1 exon 1 [len=6000] (mis=0)
208-271 ==> 0-63 on |435|TRAJ48|J-REGION| [len=63] (mis=3)
271-313 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
umis assigned: [310, 367]
of which 1 are surviving nonsolos
reads assigned: 4176
start codons at 31, 44, 47, 223
confident = false
did not find CDR3

TIG 3[bases=409]
21-299 ==> 5722-6000 on segment before TRBD1 exon 1 [len=6000] (mis=0)
307-357 ==> 0-50 on |540|TRBJ1-5|J-REGION| [len=50] (mis=3)
357-409 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
umis assigned: [17, 37, 54, 203, 211, 226, 245, 267, 304, 335] and 6 others
of which 16 are surviving nonsolos
reads assigned: 46820
start codons at 176, 186, 260, 333
confident = false
did not find CDR3
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.615 = CTCGGGACACGTTGGC-1

using 547 reads

====================================================================================

graph has 466 edges initially, 22 edges after simplification

total ucounts = 27
nonsolo ucounts = 19[2, 3, 8, 9, 10, 16, 18, 20, 28, 30, 31, 32, 36, 38, 41, 45, 48, 55, 69]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.620 = CTCGGGACAGATCTGT-1

using 59 reads

====================================================================================

graph has 4 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[59]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.622 = CTCGGGACAGCCTGTG-1

using 19 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.625 = CTCGGGACAGTCAGCC-1

using 35 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.630 = CTCGGGACATCACGAT-1

using 50 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[50]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.634 = CTCGGGACATCTCGCT-1

using 31 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[10, 21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.636 = CTCGGGACATGGGACA-1

using 23 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.639 = CTCGGGACATTTCAGG-1

using 31 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[2, 5, 23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.640 = CTCGGGAGTAAACCTC-1

using 39 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 1[35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.651 = CTCGGGAGTCAGAAGC-1

using 65 reads

====================================================================================

graph has 78 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 5[3, 4^2, 9, 43]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.657 = CTCGGGAGTCCGTGAC-1

using 9119 reads

====================================================================================

graph has 2875 edges initially, 34 edges after simplification

total ucounts = 155
nonsolo ucounts = 84[2^18, 3^10, 4^8, 5^5, 6^7, 7^7, 8, 9^4, 10, 11^2, 12^5, 14^3, 16^2, 17, 18, 64, 92, 95, 565, 591, 1361, 1780, 1966, 2075]
surviving nonsolo ucounts = 6[565, 591, 1361, 1780, 1966, 2075]
ids = [78, 2, 27, 81, 54, 60]

====================================================================================

UMI info for barcode CTCGGGAGTCCGTGAC-1 contig 1 = GGAGGACCAA...
umi AAATTACATG = 524 reads: -391X +1 -1XX +5 -2XX +3 invalidated
umi ATCACGCTTG = 1251 reads: -384X +3 -1XX +2 -1XX +1 -1XX +2 -1XX +2 -2XX +1 -1XX +1 invalidated
umi CCGTGTCTAA = 1977 reads: +403 validated
umi CGCTTAGGCC = 2089 reads: +403 validated
umi GATTCTACAT = 1791 reads: +403 validated

GOOD CONTIGS

TIG 1[bases=505]
0-50 ==> 259-309 on |630|TRBV5-1|5'UTR| [len=309] (mis=0)
50-393 ==> 0-343 on |631|TRBV5-1|L-REGION+V-REGION| [len=343] (mis=2)
394-406 ==> 0-12 on |535|TRBD1|D-REGION| [len=12] (mis=3)
405-453 ==> 0-48 on |537|TRBJ1-2|J-REGION| [len=48] (mis=5)
453-505 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 2 umis using 287 reads
cdr3 = CASSIEGQASTGGYTF at 377, score = 9 + 9
umis assigned: [2, 27, 54, 60, 81]
of which 5 are surviving nonsolos
reads assigned: 7526
start codons at 50, 332, 336
confident = true
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.664 = CTCGGGAGTGCACCAC-1

using 32 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.666 = CTCGGGAGTGTATGGG-1

using 56 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[15, 16, 24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.668 = CTCGGGAGTGTTCTTT-1

using 41 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[41]
surviving nonsolo ucounts = 1[41]
ids = [0]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.669 = CTCGGGAGTTAAGATG-1

using 894 reads

====================================================================================

graph has 880 edges initially, 26 edges after simplification

total ucounts = 114
nonsolo ucounts = 75[2^17, 3^10, 4^4, 5^5, 6^2, 7^3, 8^3, 9^3, 10^2, 11^5, 12, 16, 17^3, 18, 19^4, 20, 21, 23, 25^2, 30, 38, 39, 47, 54, 96]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.670 = CTCGGGAGTTAAGGGC-1

using 111 reads

====================================================================================

graph has 56 edges initially, 4 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[9, 47, 53]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.671 = CTCGGGAGTTACAGAA-1

using 36 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.673 = CTCGGGAGTTCAGCGC-1

using 101 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[100]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.682 = CTCGGGATCACATGCA-1

using 81 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[81]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.685 = CTCGGGATCAGATAAG-1

using 23 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[3, 6, 12]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.686 = CTCGGGATCAGCCTAA-1

using 50 reads

====================================================================================

graph has 50 edges initially, 4 edges after simplification

total ucounts = 3
nonsolo ucounts = 3[2, 19, 29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.690 = CTCGGGATCATACGGT-1

using 22134 reads

====================================================================================

graph has 8680 edges initially, 131 edges after simplification

total ucounts = 697
nonsolo ucounts = 523[2^81, 3^53, 4^46, 5^48, 6^29, 7^34, 8^21, 9^15, 10^29, 11^22, 12^18, 13^19, 14^13, 15^14, 16^6, 17^10, 18^7, 19^6, 20^3, 21^6, 22^5, 23^6, 24, 25^4, 27^3, 32^3, 34, 39, 40, 43, 52, 61, 66, 73^2, 79, 85, 113, 275, 526, 582, 960, 1747, 2094, 2328, 4216, 4386]
surviving nonsolo ucounts = 9[275, 526, 582, 960, 1747, 2094, 2328, 4216, 4386]
ids = [494, 570, 37, 18, 412, 144, 327, 592, 474]

====================================================================================

UMI info for barcode CTCGGGATCATACGGT-1 contig 1 = GTCATCCCTC...
umi AAGTGGGTGG = 961 reads: +391 validated
umi ATGCCTCCCG = 2082 reads: +391 validated
umi GCCCAGCCGC = 1759 reads: +391 validated
umi TAAGGCACTT = 276 reads: +391 validated

GOOD CONTIGS

TIG 1[bases=521]
0-78 ==> 0-78 on |595|TRBV20-1|5'UTR| [len=78] (mis=0)
78-413 ==> 0-335 on |596|TRBV20-1|L-REGION+V-REGION| [len=335] (mis=4)
422-469 ==> 0-47 on |550|TRBJ2-7|J-REGION| [len=47] (mis=2)
469-521 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 4 umis using 278 reads
cdr3 = CSATQLAPYYEQYF at 399, score = 7 + 8
umis assigned: [18, 144, 412, 494]
of which 4 are surviving nonsolos
reads assigned: 4996
start codons at 21, 78, 216, 252, 258, 271, 331
confident = true

REJECT CONTIGS

TIG 1[bases=555]
5-147 ==> 1343-1485 on segment before TRBV12-2 exon 1 [len=6488] (mis=0)
150-397 ==> 52-299 on |568|TRBV12-4|L-REGION+V-REGION| [len=347] (mis=51)
465-503 ==> 11-49 on |546|TRBJ2-3|J-REGION| [len=49] (mis=0)
503-555 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
cdr3 = CKSFRTSGKPIVQYF at 430, score = 3 + 8
umis assigned: [37, 327, 474, 570, 592]
of which 5 are surviving nonsolos
reads assigned: 11714
start codons at 29, 86, 93, 114, 137, 148, 156, 183, 197, 220, 324, 332, 366, 423
confident = false
not full
VJ delta = 16
not full
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.695 = CTCGGGATCCGATATG-1

using 36 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[2, 32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.700 = CTCGGGATCTACTCAT-1

using 104 reads

====================================================================================

graph has 78 edges initially, 2 edges after simplification

total ucounts = 12
nonsolo ucounts = 6[2, 3, 4^2, 5, 80]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.701 = CTCGGGATCTATCGCC-1

using 82 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[82]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.702 = CTCGGGATCTCGCATC-1

using 87 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[87]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!
sorting bam, mem = 0.21
running samtools sort -l 8G -o /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_T_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk45-ucc6a7a72e6/files/contig_bam_sorted.bam /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_T_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk45-ucc6a7a72e6/files/contig_bam.bam

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70.048 seconds used processing barcodes, peak mem = 0.35
