[stdout]

starting vdj_asm_asm, mem = 0.01
available mem = 830.30 GB
/scratch/etanis/software/cellranger-6.1.2/lib/bin/cr_vdj martian assembly main /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_T_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk40-ucc6a7a72e6 /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_T_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk40-ucc6a7a72e6/files /scratch/etanis/ST-118/multi/dimitri-multi/journal/SC_MULTI_CS.SC_MULTI_CORE.MULTI_GEM_WELL_PROCESSOR.VDJ_T_GEM_WELL_PROCESSOR.SC_VDJ_CONTIG_ASSEMBLER.ASSEMBLE_VDJ.fork0.chnk40.ucc6a7a72e6
n50_n50_rpu = 2159

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.0 = CGTAGGCCAGAGTGTG-1

using 19784 reads

====================================================================================

graph has 7148 edges initially, 114 edges after simplification

total ucounts = 498
nonsolo ucounts = 294[2^57, 3^40, 4^34, 5^19, 6^18, 7^17, 8^11, 9^8, 10^8, 11^6, 12^8, 13^5, 14, 15^3, 16^4, 17, 18^4, 20, 21^2, 22^2, 23, 24, 25, 26, 31, 32^2, 33, 36, 40, 44, 50^3, 54, 56, 58, 62, 65, 66, 68^2, 81, 90^2, 92^2, 94, 130, 207, 231, 270, 502, 532, 543, 616, 648, 694, 867, 913, 1109, 1233, 1237, 2866, 3973]
surviving nonsolo ucounts = 20[81, 90, 92, 130, 207, 231, 270, 502, 532, 543, 616, 648, 694, 867, 913, 1109, 1233, 1237, 2866, 3973]
ids = [344, 491, 495, 198, 367, 197, 120, 341, 289, 201, ...]

====================================================================================

UMI info for barcode CGTAGGCCAGAGTGTG-1 contig 1 = CGATGATGGA...
umi CACTATTCCC = 683 reads: +394 validated
umi CCAAAATCTA = 609 reads: +394 validated
umi GGGTGTGCTA = 504 reads: +394 validated
umi TTCTTTGCGA = 643 reads: +394 validated

UMI info for barcode CGTAGGCCAGAGTGTG-1 contig 2 = GTTCCCCTAT...
umi ATACAGTGTC = 1247 reads: +394 validated
umi ATGTAGTCGT = 251 reads: -353 +41 non-validated
umi CCCGCGCTAT = 135 reads: +377 -7 +10 non-validated
umi CCCTCCCCGC = 547 reads: +394 validated
umi GAATTGGTCA = 919 reads: +394 validated
umi GTGGGGGGGT = 1233 reads: +394 validated
umi TTTGGCCATT = 78 reads: -382 +12 non-validated

UMI info for barcode CGTAGGCCAGAGTGTG-1 contig 3 = GTTCCCCTAT...
umi ACCCTTCGTT = 870 reads: +400 validated
umi CCCCTTTCTG = 199 reads: -371X +1 -4XX +1 -8XX +1 -12XX +2 invalidated
umi GACACTTCTA = 1108 reads: +400 validated
umi GATACCCCTA = 532 reads: +400 validated
umi GTGTACCACT = 205 reads: +358 -1 +41 non-validated

GOOD CONTIGS

TIG 1[bases=506]
0-70 ==> 0-70 on |465|TRAV13-2|5'UTR| [len=70] (mis=0)
70-410 ==> 0-340 on |466|TRAV13-2|L-REGION+V-REGION| [len=340] (mis=2)
412-464 ==> 8-60 on |396|TRAJ11|J-REGION| [len=60] (mis=0)
464-506 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 4 umis using 136 reads
cdr3 = CAEMMGYSTLTF at 397, score = 9 + 8
umis assigned: [162, 181, 341, 479]
of which 4 are surviving nonsolos
reads assigned: 2392
start codons at 2, 5, 20, 41, 67, 70, 94, 289, 406, 409, 445
confident = true

TIG 2[bases=503]
0-57 ==> 0-57 on |647|TRBV6-1|5'UTR| [len=57] (mis=0)
57-391 ==> 0-334 on |648|TRBV6-1|L-REGION+V-REGION| [len=344] (mis=0)
398-410 ==> 0-12 on |535|TRBD1|D-REGION| [len=12] (mis=2)
404-451 ==> 0-47 on |550|TRBJ2-7|J-REGION| [len=47] (mis=5)
451-503 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 4 umis using 225 reads
cdr3 = CATDPGQGDEQYF at 384, score = 9 + 8
umis assigned: [100, 120, 198, 201, 271, 362, 495]
of which 7 are surviving nonsolos
reads assigned: 4316
start codons at 15, 57, 115, 168, 192, 207, 234, 298, 307
confident = true

TIG 3[bases=509]
0-57 ==> 0-57 on |647|TRBV6-1|5'UTR| [len=57] (mis=0)
57-401 ==> 0-344 on |648|TRBV6-1|L-REGION+V-REGION| [len=344] (mis=2)
407-457 ==> 0-50 on |540|TRBJ1-5|J-REGION| [len=50] (mis=5)
457-509 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 4 umis using 146 reads
cdr3 = CASSEVKGLDQPQHF at 384, score = 9 + 8
umis assigned: [45, 197, 274, 289, 367]
of which 5 are surviving nonsolos
reads assigned: 2872
start codons at 15, 57, 115, 168, 192, 207, 234, 298, 307, 433
confident = true

REJECT CONTIGS

TIG 1[bases=350]
5-147 ==> 5194-5336 on segment before TRAV8-3 exon 1 [len=5336] (mis=0)
147-308 ==> 0-161 on |522|TRAV8-3|L-REGION+V-REGION| [len=341] (mis=0)
308-350 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
umis assigned: [39]
of which 1 are surviving nonsolos
reads assigned: 2839
start codons at 147, 269, 283
confident = false
did not find CDR3

TIG 2[bases=306]
7-46 ==> 2535-2574 on segment before TRBJ2-1 exon 1 [len=3226] (mis=0)
102-204 ==> 0-102 on segment before TRBJ2-4 exon 1 [len=102] (mis=1)
204-254 ==> 0-50 on |547|TRBJ2-4|J-REGION| [len=50] (mis=0)
254-306 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
umis assigned: [212, 344, 491]
of which 3 are surviving nonsolos
reads assigned: 4045
start codons at 22
confident = false
did not find CDR3
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.5 = CGTAGGCCATACTACG-1

using 35 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.12 = CGTAGGCGTAAGGGCT-1

using 58 reads

====================================================================================

graph has 60 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[6, 14, 37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.16 = CGTAGGCGTACTTGAC-1

using 64 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[63]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.21 = CGTAGGCGTAGTGAAT-1

using 31 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.28 = CGTAGGCGTCTCACCT-1

using 95 reads

====================================================================================

graph has 84 edges initially, 4 edges after simplification

total ucounts = 14
nonsolo ucounts = 8[2, 4, 8, 9^2, 14, 20, 23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.34 = CGTAGGCGTGTTGAGG-1

using 499 reads

====================================================================================

graph has 250 edges initially, 2 edges after simplification

total ucounts = 12
nonsolo ucounts = 7[2^2, 5, 9^2, 20, 447]
surviving nonsolo ucounts = 1[447]
ids = [6]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.39 = CGTAGGCGTTCCATGA-1

using 62 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[62]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.42 = CGTAGGCGTTCTGAAC-1

using 83 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[83]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.46 = CGTAGGCGTTTCGCTC-1

using 34 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[2, 32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.49 = CGTAGGCGTTTGTTGG-1

using 99 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[99]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.50 = CGTAGGCTCAACACGT-1

using 87 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[87]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.55 = CGTAGGCTCACATAGC-1

using 679 reads

====================================================================================

graph has 508 edges initially, 10 edges after simplification

total ucounts = 44
nonsolo ucounts = 39[2^8, 3^2, 4, 5, 6^2, 7, 8, 9^2, 12^4, 14, 15, 16, 17, 18, 21^2, 22, 24, 35^2, 38, 40, 48^2, 53, 85]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.56 = CGTAGGCTCACGGTTA-1

using 61 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[61]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.59 = CGTAGGCTCAGCATGT-1

using 51 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[51]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.61 = CGTAGGCTCAGTGCAT-1

using 25 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[2, 21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.64 = CGTAGGCTCCAAACAC-1

using 16 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[16]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.65 = CGTAGGCTCCAAGCCG-1

using 134 reads

====================================================================================

graph has 200 edges initially, 4 edges after simplification

total ucounts = 21
nonsolo ucounts = 13[2^2, 4^2, 7, 8^2, 9^3, 14, 23, 27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.66 = CGTAGGCTCCAAGTAC-1

using 42 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[42]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.68 = CGTAGGCTCCACTCCA-1

using 49 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[49]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.69 = CGTAGGCTCCAGATCA-1

using 27 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.70 = CGTAGGCTCCCTGACT-1

using 59 reads

====================================================================================

graph has 52 edges initially, 6 edges after simplification

total ucounts = 9
nonsolo ucounts = 6[2^2, 3, 14, 15, 20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.84 = CGTAGGCTCTCTAGGA-1

using 87 reads

====================================================================================

graph has 72 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 6[2, 3, 4, 11, 16, 48]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.85 = CGTAGGCTCTGTCTAT-1

using 39 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[39]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.86 = CGTAGGCTCTGTTTGT-1

using 92 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[92]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.89 = CGTAGGCTCTTGTCAT-1

using 84 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[84]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.92 = CGTCACTAGAATTCCC-1

using 76 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[76]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.96 = CGTCACTAGAGCTGGT-1

using 34 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.97 = CGTCACTAGAGGGATA-1

using 59 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[59]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.100 = CGTCACTAGCATCATC-1

using 26 reads

====================================================================================

graph has 62 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.101 = CGTCACTAGCCTATGT-1

using 23620 reads

====================================================================================

graph has 8334 edges initially, 88 edges after simplification

total ucounts = 615
nonsolo ucounts = 466[2^78, 3^57, 4^45, 5^43, 6^31, 7^18, 8^19, 9^23, 10^22, 11^13, 12^13, 13^12, 14^7, 15^7, 16^5, 17^2, 18^2, 19^2, 20, 21, 22^3, 24^3, 25^4, 26^3, 28, 29, 30^3, 31, 32^2, 33^3, 34^2, 35^3, 36, 37, 38^2, 39^2, 41^2, 43^2, 44, 45, 46, 47^2, 50, 55, 60, 62, 63, 96, 115, 194, 488, 768^2, 822, 1153, 1187, 1317, 1393, 1479, 1590, 2052, 2529, 3276]
surviving nonsolo ucounts = 15[96, 194, 488, 768^2, 822, 1153, 1187, 1317, 1393, 1479, 1590, 2052, 2529, 3276]
ids = [68, 409, 547, 391, 569, 372, 217, 490, 203, 403, ...]

====================================================================================

UMI info for barcode CGTCACTAGCCTATGT-1 contig 1 = GGGACAGTGA...
umi ACAATTACTC = 1594 reads: +403 validated
umi AGCTCATCAG = 80 reads: -402X +1 invalidated
umi ATAGTCCGGT = 2056 reads: -34X +1 -5XX +1 -4XX +358 invalidated
umi CCCAAAATCT = 1 reads: -399 +1 -1X +1 -1X invalidated
umi CCGATCAGAT = 1319 reads: +403 validated
umi CCTACGTTAA = 1221 reads: +131 -1 +1 -1XX +269 invalidated
umi GGTGATCTAC = 770 reads: +403 validated
umi GTATGTGCAC = 1398 reads: +403 validated
umi GTCGTTACCG = 179 reads: -402X +1 invalidated
umi TCATTTCACT = 1178 reads: +403 validated
umi TCCTATCAAT = 1475 reads: +403 validated
umi TTAGAAACCG = 769 reads: +403 validated

UMI info for barcode CGTCACTAGCCTATGT-1 contig 2 = TGGGGACTCA...
umi GCTACGCGAG = 3432 reads: +40 -1XX +1 -4XX +1 -4XX +1 -3XX +1 -1XX +1 -9XX +1 -2X +1 -62 +1 -2XX +2 -3XX +1 -2XX +1 -3XX +1 -2XX +1 -1XX +1 -2XX +4 -4XX +227 invalidated
umi GCTTTTAGTG = 832 reads: +391 validated
umi GTGTTCTTCC = 2663 reads: +40 -1XX +1 -4XX +1 -4XX +1 -3XX +1 -1XX +1 -78XX +2 -3XX +1 -2XX +1 -3XX +1 -2XX +1 -1XX +1 -2XX +4 -4XX +227 invalidated
umi TGGCGGGATA = 493 reads: +391 validated

GOOD CONTIGS

TIG 1[bases=505]
0-50 ==> 0-50 on |678|TRBV7-3|5'UTR| [len=50] (mis=3)
50-397 ==> 0-347 on |679|TRBV7-3|L-REGION+V-REGION| [len=347] (mis=5)
404-453 ==> 0-49 on |546|TRBJ2-3|J-REGION| [len=49] (mis=1)
453-505 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 9 umis using 799 reads
cdr3 = CASSAGLARTDTQYF at 380, score = 8 + 8
umis assigned: [30, 68, 90, 193, 203, 217, 391, 403, 409, 490] and 2 others
of which 11 are surviving nonsolos
reads assigned: 11783
start codons at 50, 159, 273
confident = true

TIG 2[bases=568]
135-462 ==> 0-327 on |491|TRAV26-2|L-REGION+V-REGION| [len=327] (mis=0)
460-526 ==> 0-66 on |440|TRAJ53|J-REGION| [len=66] (mis=3)
526-568 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 3 umis using 407 reads
cdr3 = CILRDESGGSNYKLTF at 447, score = 8 + 9
umis assigned: [360, 372, 420, 547]
of which 4 are surviving nonsolos
reads assigned: 7019
start codons at 26, 33, 120, 135, 180, 187, 213, 331, 346, 360, 430
confident = true
now this is a cell
paired!

AGAGATGCTGCTGTGTACTACTGCATCCTGAGAGACGAAAGTGGAGGTAGCAACTATAAACTGACATTTGGAAAAGGAACTCTCTTAACCGTGAATCCAA <==> GAGCGGGGGGACTCAGCCGTGTATCTCTGTGCCAGCAGCGCCGGACTAGCACGCACAGATACGCAGTATTTTGGCCCAGGCACCCGGCTGACAGTGCTCG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.109 = CGTCACTAGGCATTGG-1

using 457 reads

====================================================================================

graph has 230 edges initially, 14 edges after simplification

total ucounts = 23
nonsolo ucounts = 19[2, 4, 6^3, 8^2, 10, 11, 12, 14, 19, 28, 32, 33, 47, 50, 53, 104]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.112 = CGTCACTAGGGATACC-1

using 24 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.114 = CGTCACTAGGGTATCG-1

using 87 reads

====================================================================================

graph has 72 edges initially, 4 edges after simplification

total ucounts = 8
nonsolo ucounts = 4[4, 13, 18, 48]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.115 = CGTCACTAGGTAGCTG-1

using 82 reads

====================================================================================

graph has 58 edges initially, 4 edges after simplification

total ucounts = 6
nonsolo ucounts = 5[3, 11, 16, 23, 28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.117 = CGTCACTAGGTGCTTT-1

using 65 reads

====================================================================================

graph has 60 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 7[3^2, 4, 8^2, 9, 29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.118 = CGTCACTAGGTGTGGT-1

using 51 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[51]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.119 = CGTCACTAGGTTCCTA-1

using 36 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 2[2, 31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.121 = CGTCACTAGTCAATAG-1

using 90 reads

====================================================================================

graph has 130 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 8[2, 3^2, 5, 12, 15, 18, 30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.122 = CGTCACTAGTCCATAC-1

using 5545 reads

====================================================================================

graph has 4851 edges initially, 126 edges after simplification

total ucounts = 806
nonsolo ucounts = 596[2^100, 3^85, 4^42, 5^44, 6^47, 7^36, 8^32, 9^23, 10^16, 11^20, 12^20, 13^20, 14^12, 15^14, 16^10, 17^12, 18^10, 19^7, 20^3, 21^4, 22^2, 23^6, 24^4, 25, 26, 27^3, 28^3, 29^2, 31, 33, 34, 36^2, 41, 47, 48, 50, 54, 56^2, 59^2, 64, 72, 87]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.124 = CGTCACTAGTCCGGTC-1

using 25 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.125 = CGTCACTAGTCCTCCT-1

using 45 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[45]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.128 = CGTCACTAGTGTCTCA-1

using 85 reads

====================================================================================

graph has 90 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 9[2^2, 3, 6, 7, 12^2, 15, 26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.130 = CGTCACTCAAAGCGGT-1

using 637 reads

====================================================================================

graph has 256 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 4[2, 5^2, 622]
surviving nonsolo ucounts = 1[622]
ids = [3]

====================================================================================

UMI info for barcode CGTCACTCAAAGCGGT-1 contig 1 = AGTGGTCACA...
umi TAAACGGAGG = 622 reads: +397 validated

GOOD CONTIGS

TIG 1[bases=498]
0-49 ==> 29-78 on |597|TRBV20-1|5'UTR| [len=78] (mis=0)
49-384 ==> 0-335 on |598|TRBV20-1|L-REGION+V-REGION| [len=335] (mis=1)
398-446 ==> 0-48 on |548|TRBJ2-5|J-REGION| [len=48] (mis=0)
446-498 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 1 umis using 38 reads
cdr3 = CSARALAGDYQETQYF at 370, score = 7 + 7
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 615
start codons at 49, 187, 223, 229, 242, 302
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.136 = CGTCACTCAAGTCATC-1

using 50 reads

====================================================================================

graph has 72 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 3[4, 14, 32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.138 = CGTCACTCAATCCAAC-1

using 55 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[55]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.139 = CGTCACTCACACTGCG-1

using 48 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[48]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.142 = CGTCACTCACCAACCG-1

using 50 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 3[3, 5, 38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.144 = CGTCACTCACCATCCT-1

using 117 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[117]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.148 = CGTCACTCACGGTGTC-1

using 2201 reads

====================================================================================

graph has 1845 edges initially, 50 edges after simplification

total ucounts = 190
nonsolo ucounts = 133[2^29, 3^17, 4^12, 5^12, 6^13, 7^10, 8^5, 9^6, 11, 12^3, 14, 15, 16^3, 17, 18, 19, 21^2, 23^3, 24, 26, 28^2, 36, 41, 42, 44, 56, 80, 92, 899]
surviving nonsolo ucounts = 1[899]
ids = [156]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.153 = CGTCACTCAGAGTGTG-1

using 74 reads

====================================================================================

graph has 66 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[3, 71]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.155 = CGTCACTCAGCCAGAA-1

using 12816 reads

====================================================================================

graph has 5640 edges initially, 100 edges after simplification

total ucounts = 596
nonsolo ucounts = 426[2^92, 3^45, 4^41, 5^37, 6^21, 7^23, 8^17, 9^19, 10^12, 11^15, 12^11, 13^5, 14^11, 15^4, 16^5, 17^5, 18^5, 19^3, 20^3, 22^2, 23^3, 24^4, 25^2, 26, 27, 28^4, 29^2, 30, 31, 32^2, 33, 34, 35, 36, 37, 38, 39, 40^2, 41, 44^3, 45, 46, 47^2, 48, 50, 53, 54, 60^2, 64, 70, 77, 1657, 2664, 4162]
surviving nonsolo ucounts = 3[1657, 2664, 4162]
ids = [146, 18, 356]

====================================================================================

REJECT CONTIGS

TIG 1[bases=306]
4-77 ==> 727-800 on segment before TRAJ12 exon 1 [len=800] (mis=0)
200-264 ==> 0-64 on |395|TRAJ10|J-REGION| [len=64] (mis=0)
264-306 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
umis assigned: [356, 418]
of which 1 are surviving nonsolos
reads assigned: 4125
start codons at 23, 32, 79
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.157 = CGTCACTCAGCGATCC-1

using 44067 reads

====================================================================================

graph has 24676 edges initially, 1076 edges after simplification

total ucounts = 1801
nonsolo ucounts = 1594[2^108, 3^72, 4^67, 5^62, 6^49, 7^49, 8^47, 9^60, 10^39, 11^47, 12^28, 13^35, 14^27, 15^23, 16^27, 17^35, 18^24, 19^30, 20^24, 21^17, 22^25, 23^17, 24^17, 25^25, 26^16, 27^16, 28^20, 29^13, 30^12, 31^9, 32^17, 33^16, 34^23, 35^10, 36^16, 37^13, 38^10, 39^20, 40^13, 41^13, 42^12, 43^13, 44^11, 45^11, 46^10, 47^7, 48^9, 49^4, 50^21, 51^10, 52^9, 53^8, 54^13, 55^9, 56^12, 57^11, 58^8, 59^10, 60^8, 61^6, 62^10, 63^11, 64^6, 65^5, 66^7, 67^5, 68^10, 69^5, 70^11, 71^8, 72^6, 73^8, 74^8, 75^4, 76^7, 77^4, 78^10, 79^4, 80^5, 81^2, 82^4, 83^5, 84^3, 85^5, 86^4, 87^5, 88^2, 89, 90^4, 91, 92^3, 93, 94, 95, 96, 97^4, 98^2, 99^5, 102, 104, 105^2, 106, 108^2, 112^2, 120^2, 180, 213]
surviving nonsolo ucounts = 4[51, 97, 105, 213]
ids = [1708, 1709, 1139, 134]

====================================================================================

REJECT CONTIGS

TIG 1[bases=316]
1-63 ==> 42-104 on segment before TRBJ2-3 exon 1 [len=104] (mis=0)
63-112 ==> 0-49 on |546|TRBJ2-3|J-REGION| [len=49] (mis=0)
112-214 ==> 0-102 on segment before TRBJ2-4 exon 1 [len=102] (mis=1)
264-316 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
umis assigned: [134, 625, 695, 859, 1139, 1615, 1708, 1709]
of which 4 are surviving nonsolos
reads assigned: 254
start codons at 
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.158 = CGTCACTCAGCGTAAG-1

using 21 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.159 = CGTCACTCAGCTATTG-1

using 66 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 3[2, 5, 59]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.162 = CGTCACTCAGCTGTTA-1

using 71 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[71]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.169 = CGTCACTCAGTCGTGC-1

using 88 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[88]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.173 = CGTCACTCATCGTCGG-1

using 262 reads

====================================================================================

graph has 158 edges initially, 6 edges after simplification

total ucounts = 19
nonsolo ucounts = 15[2^2, 3^2, 4, 7^2, 9, 10, 11, 21, 27, 46, 51, 55]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.174 = CGTCACTCATGACATC-1

using 184 reads

====================================================================================

graph has 74 edges initially, 4 edges after simplification

total ucounts = 7
nonsolo ucounts = 3[4, 42, 134]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.176 = CGTCACTCATGTTGAC-1

using 18850 reads

====================================================================================

graph has 6950 edges initially, 84 edges after simplification

total ucounts = 587
nonsolo ucounts = 427[2^72, 3^65, 4^33, 5^28, 6^39, 7^31, 8^22, 9^29, 10^12, 11^12, 12^6, 13^9, 14^4, 15^9, 16^6, 17^3, 18, 19^2, 21^2, 22^3, 24^3, 25^2, 26^2, 27^2, 28, 29, 31, 32, 33, 36^2, 37, 38, 39, 44, 45, 48, 59, 66, 67, 68, 75, 97, 808, 825, 928, 1085, 1149, 1173, 1208, 1462, 1713, 2271, 2490]
surviving nonsolo ucounts = 11[808, 825, 928, 1085, 1149, 1173, 1208, 1462, 1713, 2271, 2490]
ids = [216, 364, 477, 498, 333, 512, 90, 352, 65, 75, ...]

====================================================================================

UMI info for barcode CGTCACTCATGTTGAC-1 contig 1 = AGAATGCTTA...
umi AGATGTAAGA = 1728 reads: +406 validated
umi ATACACCGAT = 1208 reads: +406 validated
umi CGGGCACTTA = 809 reads: +406 validated
umi GCGTCCCTTT = 1147 reads: +406 validated
umi GGCGATTATA = 1479 reads: +406 validated
umi GGTCCTGGCT = 839 reads: +406 validated
umi TCTACCTTAA = 927 reads: +406 validated
umi TGCTACATGA = 1177 reads: +406 validated

UMI info for barcode CGTCACTCATGTTGAC-1 contig 2 = AGGCAACCCA...
umi TGAATGTAGG = 1095 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=505]
0-47 ==> 0-47 on |692|TRBV9|5'UTR| [len=47] (mis=0)
47-390 ==> 0-343 on |693|TRBV9|L-REGION+V-REGION| [len=343] (mis=0)
404-453 ==> 0-49 on |546|TRBJ2-3|J-REGION| [len=49] (mis=0)
453-505 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 8 umis using 598 reads
cdr3 = CASSVGLGHGSTDTQYF at 374, score = 9 + 8
umis assigned: [65, 90, 216, 333, 352, 364, 477, 512]
of which 8 are surviving nonsolos
reads assigned: 9155
start codons at 3, 47, 169, 255
confident = true

TIG 2[bases=467]
0-43 ==> 33-76 on |487|TRAV25|5'UTR| [len=76] (mis=0)
43-370 ==> 0-327 on |488|TRAV25|L-REGION+V-REGION| [len=327] (mis=5)
371-425 ==> 8-62 on |428|TRAJ41|J-REGION| [len=62] (mis=0)
425-467 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 1 umis using 85 reads
cdr3 = CAIDSGYALNF at 361, score = 6 + 7
umis assigned: [498]
of which 1 are surviving nonsolos
reads assigned: 1077
start codons at 25, 43, 61, 72, 76, 95, 109, 131, 344, 380
confident = true

REJECT CONTIGS

TIG 1[bases=316]
8-208 ==> 5800-6000 on segment before TRBD1 exon 1 [len=6000] (mis=0)
214-264 ==> 0-50 on |540|TRBJ1-5|J-REGION| [len=50] (mis=3)
264-316 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
umis assigned: [75, 192]
of which 2 are surviving nonsolos
reads assigned: 4717
start codons at 85, 95, 169, 240
confident = false
did not find CDR3
now this is a cell
paired!

ACAGCCACCCAGACTACAGATGTAGGAACCTACTTCTGTGCTATAGATTCCGGGTATGCACTCAACTTCGGCAAAGGCACCTCGCTGTTGGTCACACCCC <==> GGGGACTCAGCTTTGTATTTCTGTGCCAGCAGCGTAGGCTTGGGGCACGGTAGCACAGATACGCAGTATTTTGGCCCAGGCACCCGGCTGACAGTGCTCG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.177 = CGTCACTCATTAGCCA-1

using 103 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[103]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.179 = CGTCACTCATTCACTT-1

using 190 reads

====================================================================================

graph has 208 edges initially, 8 edges after simplification

total ucounts = 17
nonsolo ucounts = 12[2^4, 6, 9, 11, 18, 20, 36, 38, 39]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.187 = CGTCACTGTAAGTAGT-1

using 43970 reads

====================================================================================

graph has 14059 edges initially, 242 edges after simplification

total ucounts = 1144
nonsolo ucounts = 812[2^138, 3^83, 4^77, 5^70, 6^63, 7^41, 8^29, 9^30, 10^31, 11^32, 12^27, 13^19, 14^14, 15^16, 16^17, 17^15, 18^13, 19^10, 20^12, 21^4, 22^8, 23^6, 24^5, 25^3, 26^4, 27, 31^2, 32^2, 34, 37, 42, 43, 44, 45^2, 47, 49, 50, 51, 53, 54, 59, 60^4, 62, 72, 79, 98, 220, 390, 442, 496, 627, 652, 656, 701, 943, 1082, 1223, 1608, 2672, 3645, 4900, 5167, 5231, 5718]
surviving nonsolo ucounts = 15[496, 627, 652, 656, 701, 943, 1082, 1223, 1608, 2672, 3645, 4900, 5167, 5231, 5718]
ids = [61, 323, 1118, 844, 1036, 1022, 301, 52, 249, 1124, ...]

====================================================================================

UMI info for barcode CGTCACTGTAAGTAGT-1 contig 1 = GAGAAGCCTC...
umi AATGGCCCCT = 472 reads: -379 +2 -1 +1 -1X +2 -1XX +2 -4XX +2 -1XX +1 -2XX +2 -4XX +1 -1XX +1 -1XX invalidated
umi ACACCGCCGA = 493 reads: +383 -6 +20 non-validated
umi CATTCAGGGA = 627 reads: +409 validated
umi TATCCTACAT = 668 reads: +409 validated
umi TTAATATTCG = 709 reads: +356 -2XX +1 -2XX +1 -1X +2 -2 +2 -1 +39 invalidated

UMI info for barcode CGTCACTGTAAGTAGT-1 contig 2 = ATTCTTTCTT...
umi CATAGTGTTT = 1086 reads: +397 validated
umi TGTGGCCTTA = 907 reads: +397 validated

GOOD CONTIGS

TIG 1[bases=568]
0-117 ==> 0-117 on |474|TRAV19|5'UTR| [len=117] (mis=1)
117-467 ==> 0-350 on |475|TRAV19|L-REGION+V-REGION| [len=350] (mis=1)
470-526 ==> 8-64 on |395|TRAJ10|J-REGION| [len=64] (mis=0)
526-568 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 2 umis using 56 reads
cdr3 = CALSEAKGGGNKLTF at 450, score = 7 + 8
umis assigned: [52, 61, 323, 844, 1036]
of which 5 are surviving nonsolos
reads assigned: 2919
start codons at 111, 117, 174, 229, 250, 340, 349
confident = true

TIG 2[bases=469]
0-20 ==> 0-20 on |612|TRBV28|5'UTR| [len=20] (mis=0)
20-364 ==> 0-344 on |613|TRBV28|L-REGION+V-REGION| [len=344] (mis=3)
367-417 ==> 0-50 on |540|TRBJ1-5|J-REGION| [len=50] (mis=1)
417-469 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 2 umis using 123 reads
cdr3 = CASSSPGSNQPQHF at 347, score = 6 + 8
umis assigned: [301, 1022]
of which 2 are surviving nonsolos
reads assigned: 1959
start codons at 20, 78, 142, 155, 162, 170, 225, 228, 236, 338, 393
confident = true

REJECT CONTIGS

TIG 1[bases=464]
5-200 ==> 5805-6000 on segment before TRBD1 exon 1 [len=6000] (mis=0)
361-412 ==> 0-51 on |539|TRBJ1-4|J-REGION| [len=51] (mis=0)
412-464 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
umis assigned: [249]
of which 1 are surviving nonsolos
reads assigned: 1583
start codons at 77, 87, 161, 209, 213, 353, 367
confident = false
did not find CDR3

TIG 2[bases=408]
25-301 ==> 5724-6000 on segment before TRBD1 exon 1 [len=6000] (mis=0)
308-356 ==> 0-48 on |536|TRBJ1-1|J-REGION| [len=48] (mis=4)
356-408 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
umis assigned: [332, 539, 603, 738, 1041, 1118, 1124]
of which 7 are surviving nonsolos
reads assigned: 27587
start codons at 19, 178, 188, 262, 310, 314
confident = false
did not find CDR3
now this is a cell
paired!

GTCGTGGACTCAGCAGTATACTTCTGTGCTCTGAGTGAGGCGAAGGGAGGAGGAAACAAACTCACCTTTGGGACAGGCACTCAGCTAAAAGTGGAACTCA <==> GCCAGCACCAACCAGACATCTATGTACCTCTGTGCCAGCAGTTCACCGGGGAGCAATCAGCCCCAGCATTTTGGTGATGGGACTCGACTCTCCATCCTAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.191 = CGTCACTGTACGCTGC-1

using 80 reads

====================================================================================

graph has 70 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[36, 43]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.192 = CGTCACTGTACTCAAC-1

using 52 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[52]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.195 = CGTCACTGTAGAGGAA-1

using 30 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.202 = CGTCACTGTATTCTCT-1

using 98 reads

====================================================================================

graph has 74 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 5[3^2, 9, 24, 57]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.209 = CGTCACTGTCCGAGTC-1

using 27 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.211 = CGTCACTGTCGAATCT-1

using 2309 reads

====================================================================================

graph has 1881 edges initially, 68 edges after simplification

total ucounts = 396
nonsolo ucounts = 264[2^59, 3^39, 4^28, 5^23, 6^19, 7^14, 8^12, 9^12, 10^5, 11^7, 12, 13^6, 14^3, 15^3, 16^7, 18, 19^3, 22, 23^2, 24, 25, 29, 32^2, 33^2, 34^2, 36, 39^2, 40^2, 42, 43, 46, 56, 82]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.212 = CGTCACTGTCGACTGC-1

using 73 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[73]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.213 = CGTCACTGTCGCTTTC-1

using 45 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[45]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.224 = CGTCACTGTGCCTTGG-1

using 403 reads

====================================================================================

graph has 276 edges initially, 14 edges after simplification

total ucounts = 30
nonsolo ucounts = 24[2, 3^2, 4^2, 5^3, 6, 7^3, 8, 13, 14, 17, 19, 22, 27^2, 28, 39, 57, 68]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.228 = CGTCACTGTGTTCTTT-1

using 46 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[46]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.229 = CGTCACTGTGTTTGTG-1

using 108 reads

====================================================================================

graph has 94 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 7[4^2, 5, 7, 23, 24, 39]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.232 = CGTCACTGTTATCCGA-1

using 19 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.233 = CGTCACTGTTATCGGT-1

using 8356 reads

====================================================================================

graph has 7066 edges initially, 188 edges after simplification

total ucounts = 1009
nonsolo ucounts = 719[2^130, 3^88, 4^82, 5^65, 6^38, 7^38, 8^32, 9^27, 10^26, 11^27, 12^27, 13^7, 14^17, 15^10, 16^12, 17^12, 18^9, 19^6, 20^5, 21^3, 22^6, 23^3, 24^3, 25^3, 26^3, 27^3, 28, 29^5, 30, 31, 33^3, 34^2, 35^3, 37^2, 39, 40^2, 41, 43^3, 44, 51, 54^2, 57, 59, 60, 83, 88^2, 104, 1583]
surviving nonsolo ucounts = 1[1583]
ids = [698]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.242 = CGTCACTGTTGCTCCT-1

using 29 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.246 = CGTCACTTCAACGAAA-1

using 23 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.249 = CGTCACTTCACGCGGT-1

using 28 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 2[2, 23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.251 = CGTCACTTCAGAGCTT-1

using 21 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[3^2, 14]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.255 = CGTCACTTCAGGCCCA-1

using 948 reads

====================================================================================

graph has 280 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[2^2, 943]
surviving nonsolo ucounts = 1[943]
ids = [3]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.259 = CGTCACTTCATTGCCC-1

using 89 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[89]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.261 = CGTCACTTCCACGTTC-1

using 74 reads

====================================================================================

graph has 58 edges initially, 4 edges after simplification

total ucounts = 14
nonsolo ucounts = 7[2^2, 3, 4, 6, 25^2]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.263 = CGTCACTTCCAGTAGT-1

using 56 reads

====================================================================================

graph has 66 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 7[2, 3^2, 6, 8, 9, 21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.265 = CGTCACTTCCCAAGAT-1

using 45 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[45]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.266 = CGTCACTTCCCAGGTG-1

using 73 reads

====================================================================================

graph has 62 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 4[3^2, 4, 63]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.269 = CGTCACTTCCGTACAA-1

using 104 reads

====================================================================================

graph has 82 edges initially, 4 edges after simplification

total ucounts = 7
nonsolo ucounts = 5[2, 3, 8, 22, 67]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.272 = CGTCACTTCGACAGCC-1

using 58 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[2, 5, 49]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.273 = CGTCACTTCGCCATAA-1

using 24 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.275 = CGTCACTTCGCTAGCG-1

using 98 reads

====================================================================================

graph has 92 edges initially, 4 edges after simplification

total ucounts = 10
nonsolo ucounts = 5[2^2, 9, 27, 53]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.279 = CGTCACTTCTCGCATC-1

using 14 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[13]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.282 = CGTCACTTCTGGGCCA-1

using 134 reads

====================================================================================

graph has 54 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[134]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.284 = CGTCACTTCTGTCCGT-1

using 79 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[79]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.285 = CGTCACTTCTGTTTGT-1

using 19 reads

====================================================================================

graph has 4 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.287 = CGTCACTTCTTGAGAC-1

using 22 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.288 = CGTCACTTCTTGTCAT-1

using 79 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[4, 17, 56]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.292 = CGTCAGGAGACACGAC-1

using 18 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.296 = CGTCAGGAGAGATGAG-1

using 56 reads

====================================================================================

graph has 60 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 7[2, 3, 5, 7^2, 12, 17]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.299 = CGTCAGGAGATCACGG-1

using 44 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 4[2^2, 4, 35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.303 = CGTCAGGAGCAACGGT-1

using 55 reads

====================================================================================

graph has 68 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 5[3, 4^2, 15, 27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.307 = CGTCAGGAGCCCTAAT-1

using 213 reads

====================================================================================

graph has 150 edges initially, 2 edges after simplification

total ucounts = 48
nonsolo ucounts = 27[2^5, 3^4, 4^2, 5^4, 6^3, 7^2, 8, 15^2, 16^2, 19, 21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.308 = CGTCAGGAGCCTCGTG-1

using 104 reads

====================================================================================

graph has 154 edges initially, 2 edges after simplification

total ucounts = 15
nonsolo ucounts = 14[2, 3^3, 5^3, 6, 9, 10^2, 12, 14, 16]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.309 = CGTCAGGAGCGAAGGG-1

using 61 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[61]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.313 = CGTCAGGAGCTTCGCG-1

using 4628 reads

====================================================================================

graph has 3771 edges initially, 130 edges after simplification

total ucounts = 636
nonsolo ucounts = 431[2^89, 3^70, 4^48, 5^36, 6^25, 7^20, 8^18, 9^10, 10^8, 11^10, 12^4, 13^7, 14^5, 15^4, 16^3, 17^2, 18^3, 19^5, 20^4, 21^3, 22^5, 23^3, 24^5, 25^4, 26^4, 29, 30^2, 31^2, 32^3, 33^2, 37^2, 38^2, 40^2, 41^3, 48, 51^3, 52, 53, 54, 59, 61, 62, 64, 71, 72, 73, 79, 114, 122]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.315 = CGTCAGGAGGAGTTTA-1

using 74 reads

====================================================================================

graph has 136 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 5[2^2, 3, 6, 60]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.317 = CGTCAGGAGGCGACAT-1

using 594 reads

====================================================================================

graph has 429 edges initially, 14 edges after simplification

total ucounts = 33
nonsolo ucounts = 29[2^5, 3^2, 4^3, 5^2, 6^2, 8, 9, 13, 21, 27, 30, 39, 42^2, 43, 46, 50, 51, 52, 67]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.320 = CGTCAGGAGGGAGTAA-1

using 1335 reads

====================================================================================

graph has 1150 edges initially, 28 edges after simplification

total ucounts = 182
nonsolo ucounts = 121[2^18, 3^28, 4^13, 5^6, 6^6, 7^10, 8^7, 10^3, 11^4, 13, 14, 15^6, 16, 17, 18^2, 19, 23, 25^3, 26, 27, 28, 32, 37, 47, 67, 102, 167]
surviving nonsolo ucounts = 1[167]
ids = [116]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.322 = CGTCAGGAGGTCATCT-1

using 133 reads

====================================================================================

graph has 127 edges initially, 6 edges after simplification

total ucounts = 7
nonsolo ucounts = 6[2, 4, 6, 19, 25, 76]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.325 = CGTCAGGAGTCGAGTG-1

using 53 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 1[51]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.327 = CGTCAGGAGTCTCGGC-1

using 17 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[17]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.329 = CGTCAGGAGTGGACGT-1

using 79 reads

====================================================================================

graph has 102 edges initially, 4 edges after simplification

total ucounts = 13
nonsolo ucounts = 6[2, 3, 7^2, 24, 29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.333 = CGTCAGGAGTTAGGTA-1

using 82 reads

====================================================================================

graph has 167 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[17, 65]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.334 = CGTCAGGAGTTCGCAT-1

using 68 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 6[2, 3^2, 14, 22^2]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.335 = CGTCAGGAGTTTAGGA-1

using 80 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[80]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.340 = CGTCAGGCAACACCTA-1

using 3382 reads

====================================================================================

graph has 3094 edges initially, 74 edges after simplification

total ucounts = 493
nonsolo ucounts = 313[2^71, 3^51, 4^32, 5^28, 6^24, 7^18, 8^20, 9^7, 10^11, 11^3, 12^3, 13^4, 14^4, 15^4, 17, 18, 21^2, 23, 24, 25^2, 27, 28^3, 31^2, 33, 34, 37, 40^3, 43, 45, 50^2, 56, 57, 60, 65, 72, 81, 82, 268, 299]
surviving nonsolo ucounts = 4[60, 82, 268, 299]
ids = [208, 108, 328, 115]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.342 = CGTCAGGCAAGCCTAT-1

using 73 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[73]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.343 = CGTCAGGCAAGCTGTT-1

using 74 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[74]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.346 = CGTCAGGCAATCGGTT-1

using 98 reads

====================================================================================

graph has 80 edges initially, 4 edges after simplification

total ucounts = 13
nonsolo ucounts = 10[2, 3, 4^2, 5, 9, 11^2, 14, 32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.353 = CGTCAGGCACGGATAG-1

using 31 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 3[2, 3, 26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.357 = CGTCAGGCACTTCTGC-1

using 29 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.358 = CGTCAGGCAGACAGGT-1

using 7 reads

====================================================================================

graph has 4 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[7]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.361 = CGTCAGGCAGCCTTTC-1

using 101 reads

====================================================================================

graph has 54 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[101]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.371 = CGTCAGGCATGTCCTC-1

using 45 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[45]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.375 = CGTCAGGCATTGGTAC-1

using 66 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[2, 3, 59]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.379 = CGTCAGGGTACTTAGC-1

using 291 reads

====================================================================================

graph has 355 edges initially, 10 edges after simplification

total ucounts = 25
nonsolo ucounts = 20[2^5, 3, 4^2, 5^3, 6^2, 7, 12, 17, 25, 29, 73, 75]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.381 = CGTCAGGGTAGCTAAA-1

using 4028 reads

====================================================================================

graph has 1030 edges initially, 2 edges after simplification

total ucounts = 16
nonsolo ucounts = 11[2^3, 3^2, 4, 5, 9, 11, 16, 3966]
surviving nonsolo ucounts = 1[3966]
ids = [5]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.383 = CGTCAGGGTATAATGG-1

using 29 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.394 = CGTCAGGGTCTCGTTC-1

using 31 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.395 = CGTCAGGGTCTCTCTG-1

using 38 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.397 = CGTCAGGGTGCAACTT-1

using 111 reads

====================================================================================

graph has 66 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 5[2^2, 6, 9, 88]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.399 = CGTCAGGGTGCGAAAC-1

using 43 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[43]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.400 = CGTCAGGGTGCGGTAA-1

using 82 reads

====================================================================================

graph has 56 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[82]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.402 = CGTCAGGGTGGCTCCA-1

using 76 reads

====================================================================================

graph has 54 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 3[2, 4, 70]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.404 = CGTCAGGGTGTGCCTG-1

using 31 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.406 = CGTCAGGGTGTTCTTT-1

using 31 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.410 = CGTCAGGGTTATTCTC-1

using 46 reads

====================================================================================

graph has 52 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 6[2^2, 3, 4, 9, 21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.418 = CGTCAGGTCACCACCT-1

using 106 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[106]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.420 = CGTCAGGTCACTGGGC-1

using 31 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.422 = CGTCAGGTCAGCACAT-1

using 35 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.424 = CGTCAGGTCATCGATG-1

using 59 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[59]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.428 = CGTCAGGTCCACGAAT-1

using 1039 reads

====================================================================================

graph has 802 edges initially, 18 edges after simplification

total ucounts = 56
nonsolo ucounts = 41[2^5, 3^3, 4^4, 6, 7^2, 8^2, 10, 11^2, 12, 14, 15^2, 19, 21^2, 23, 28, 39, 42, 55, 58, 61, 63^2, 66, 68, 70, 84^2]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.431 = CGTCAGGTCCCTTGTG-1

using 22 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.432 = CGTCAGGTCCGCATCT-1

using 32 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.433 = CGTCAGGTCCGTCATC-1

using 76 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[3, 71]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.434 = CGTCAGGTCCTACAGA-1

using 44 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[2, 42]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.438 = CGTCAGGTCGCGGATC-1

using 223 reads

====================================================================================

graph has 190 edges initially, 4 edges after simplification

total ucounts = 16
nonsolo ucounts = 10[2, 4, 11, 14^2, 17, 23, 28, 34, 70]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.444 = CGTCAGGTCTAACTTC-1

using 67 reads

====================================================================================

graph has 90 edges initially, 4 edges after simplification

total ucounts = 7
nonsolo ucounts = 7[2^2, 3, 5, 6, 24, 25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.456 = CGTCAGGTCTTCGAGA-1

using 62 reads

====================================================================================

graph has 52 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[62]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.459 = CGTCCATAGAAGATTC-1

using 5345 reads

====================================================================================

graph has 3742 edges initially, 182 edges after simplification

total ucounts = 597
nonsolo ucounts = 441[2^71, 3^56, 4^43, 5^40, 6^20, 7^26, 8^15, 9^12, 10^11, 11^12, 12^9, 13^7, 14^7, 15^10, 16^6, 17^5, 18^4, 19^7, 20^6, 21^8, 22^6, 23^3, 24^2, 25, 26^4, 27^6, 28^6, 29^2, 31^2, 34, 35, 36^4, 37, 38, 39^2, 40, 42^2, 43, 44, 45, 46^2, 49, 52^2, 54, 57, 58, 59, 60, 61, 67, 74, 75, 79, 85, 93, 97]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.460 = CGTCCATAGAATTCCC-1

using 42253 reads

====================================================================================

graph has 12244 edges initially, 182 edges after simplification

total ucounts = 813
nonsolo ucounts = 596[2^102, 3^67, 4^55, 5^53, 6^50, 7^39, 8^22, 9^20, 10^14, 11^15, 12^22, 13^12, 14^15, 15^7, 16^9, 17^5, 18^7, 19^10, 20^4, 21, 22^5, 23^6, 25^7, 26^2, 27, 28^3, 29, 30^3, 31^2, 32, 33^3, 34^3, 36^2, 44^3, 45, 51, 67^2, 78, 87, 239, 278, 917, 1094, 1256, 1257, 1260, 1264, 1270, 1406, 1433, 1505, 1586, 1718, 1739, 3175, 4424, 5114, 5997]
surviving nonsolo ucounts = 18[87, 278, 917, 1094, 1256, 1257, 1264, 1270, 1406, 1433, 1505, 1586, 1718, 1739, 3175, 4424, 5114, 5997]
ids = [671, 461, 652, 571, 538, 398, 691, 805, 503, 140, ...]

====================================================================================

UMI info for barcode CGTCCATAGAATTCCC-1 contig 1 = GGGGAGAGCA...
umi ATCTTCTTCT = 1725 reads: +412 validated
umi CCCTAACTCC = 1743 reads: +412 validated
umi GACAATCTAC = 494 reads: -90 +4 -2XX +8 -4XX +3 -1XX +7 -1XX +3 -3XX +20 -2XX +1 -1XX +1 -1XX +4 -2XX +1 -1XX +1 -1XX +17 -201XX +4 -1X +4 -1XX +2 -4XX +2 -1XX +1 -2XX +3 -1XX +1 -1XX +2 -2XX invalidated
umi GCGACAACGG = 1595 reads: +412 validated
umi GCGTCGTTCG = 276 reads: +412 validated
umi TCTTAACAGA = 84 reads: +362 -1 +5 -1 +1 -23 +19 non-validated
umi TGATGACATA = 1504 reads: +412 validated

UMI info for barcode CGTCCATAGAATTCCC-1 contig 2 = ATCAATGCAC...
umi AGCTCCGGGG = 1439 reads: +406 validated
umi GACGTTTCGG = 1273 reads: +406 validated
umi GGTCTCGCAA = 1409 reads: +406 validated
umi GTGTCGTAAG = 1259 reads: +406 validated
umi TACGGTGTCT = 1108 reads: +406 validated
umi TCGTGTACCT = 911 reads: +406 validated
umi TGATCTCTGT = 1238 reads: +406 validated
umi TTTGTTGACC = 1279 reads: +406 validated

GOOD CONTIGS

TIG 1[bases=499]
45-395 ==> 0-350 on |505|TRAV38-1|L-REGION+V-REGION| [len=350] (mis=1)
391-457 ==> 0-66 on |432|TRAJ45|J-REGION| [len=66] (mis=4)
457-499 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 5 umis using 433 reads
cdr3 = CAFMKPSGGGADGLTF at 378, score = 7 + 8
umis assigned: [190, 256, 386, 454, 461, 671, 692]
of which 6 are surviving nonsolos
reads assigned: 7314
start codons at 45, 102, 135, 178, 237, 277, 369, 387
confident = true

TIG 2[bases=504]
0-46 ==> 0-46 on |661|TRBV6-6|5'UTR| [len=46] (mis=0)
46-390 ==> 0-344 on |662|TRBV6-6|L-REGION+V-REGION| [len=344] (mis=5)
394-406 ==> 0-12 on |535|TRBD1|D-REGION| [len=12] (mis=2)
408-452 ==> 9-53 on |542|TRBJ1-6|J-REGION| [len=53] (mis=0)
452-504 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 8 umis using 645 reads
cdr3 = CASSDSPGTGAGSPLHF at 373, score = 9 + 9
umis assigned: [140, 398, 503, 538, 571, 652, 691, 805]
of which 8 are surviving nonsolos
reads assigned: 9727
start codons at 4, 46, 104, 157, 181, 196, 223, 287, 428
confident = true

REJECT CONTIGS

TIG 1[bases=361]
5-251 ==> 5754-6000 on segment before TRBD1 exon 1 [len=6000] (mis=0)
261-309 ==> 0-48 on |536|TRBJ1-1|J-REGION| [len=48] (mis=2)
309-361 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
umis assigned: [148, 198, 384, 508, 747]
of which 4 are surviving nonsolos
reads assigned: 18129
start codons at 128, 138, 212, 260
confident = false
did not find CDR3
now this is a cell
paired!

GGGGACACTGCGATGTATTTCTGTGCTTTCATGAAGCCCTCAGGAGGAGGTGCTGACGGACTCACCTTTGGCAAAGGGACTCATCTAATCATCCAGCCCT <==> TCCCAGACATCTGTGTACTTCTGTGCCAGCAGTGACAGTCCCGGGACAGGGGCAGGTTCACCCCTCCACTTTGGGAATGGGACCAGGCTCACTGTGACAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.461 = CGTCCATAGACAAAGG-1

using 44 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[2^2, 12, 26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.466 = CGTCCATAGAGTACCG-1

using 28 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.469 = CGTCCATAGATATGGT-1

using 105 reads

====================================================================================

graph has 132 edges initially, 2 edges after simplification

total ucounts = 15
nonsolo ucounts = 10[2, 4^3, 5^2, 6, 14, 22, 34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.470 = CGTCCATAGATCGGGT-1

using 20 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.471 = CGTCCATAGATGGCGT-1

using 25 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 3[2, 4, 15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.489 = CGTCCATAGGATTCGG-1

using 46 reads

====================================================================================

graph has 70 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[2, 44]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.492 = CGTCCATAGGCTCAGA-1

using 31126 reads

====================================================================================

graph has 12070 edges initially, 144 edges after simplification

total ucounts = 1124
nonsolo ucounts = 812[2^145, 3^97, 4^82, 5^70, 6^60, 7^52, 8^38, 9^41, 10^20, 11^29, 12^22, 13^16, 14^8, 15^12, 16^7, 17^11, 18^7, 19^6, 20^6, 21^8, 22^5, 23^6, 24^4, 25^3, 26^4, 28^3, 29^3, 30^2, 31^5, 32^2, 34, 35, 36, 39, 42^2, 43, 44, 46, 54, 56, 62, 75^2, 77, 86, 115, 178, 289, 387, 415, 453, 496, 589, 618, 635, 723, 740, 774, 853, 909, 1140, 1275, 1536, 1767, 1918, 2182, 6237]
surviving nonsolo ucounts = 19[289, 387, 415, 453, 496, 589, 618, 635, 723, 740, 774, 853, 1140, 1275, 1536, 1767, 1918, 2182, 6237]
ids = [794, 684, 195, 1071, 247, 649, 385, 351, 425, 62, ...]

====================================================================================

UMI info for barcode CGTCCATAGGCTCAGA-1 contig 1 = TGGGACACAT...
umi AGGCTCTAGC = 790 reads: -378X +3 -2XX +1 -5XX +2 -1XX +2 invalidated
umi CACTATTCCG = 500 reads: +371 -11 +1 -2 +9 non-validated
umi CATCAGCATC = 1949 reads: +394 validated
umi CCCTTCGTCT = 627 reads: +394 validated
umi CGCTGATTGA = 648 reads: -366X +1 -9XX +3 -4XX +1 -8XX +2 invalidated
umi CGGCATAGTA = 723 reads: +394 validated
umi CTGGTAACCC = 1286 reads: +394 validated
umi GGCGCATATA = 583 reads: +375 -14 +5 non-validated
umi GGTGGCCCTT = 386 reads: +305 -1 +4 -2 +2 -1 +13 -1 +6 -1 +7 -45 +6 non-validated
umi TACTCCACAT = 289 reads: +358 -1 +3 -1 +1 -3X +4 -1 +3 -19 invalidated
umi TATATTCTCT = 779 reads: +394 validated
umi TCCAAGGGAA = 1392 reads: -320X +1 -1XX +1 -8XX +1 -5XX +1 -5XX +2 -3XX +1 -1XX +1 -1XX +42 invalidated
umi TCTACATCCT = 5024 reads: -356 +1 -2XX +1 -2XX +3 -1XX +2 -4XX +9 -1XX +1 -1XX +2 -1XX +2 -3XX +2 invalidated
umi TTGAACGACC = 459 reads: +381 -1 +12 non-validated

UMI info for barcode CGTCCATAGGCTCAGA-1 contig 2 = CCGGTGTCGG...
umi ACGCTTTGGA = 712 reads: +370 -13 +17 non-validated
umi ATTACTCTGT = 399 reads: +335 -2 +2 -44 +17 non-validated
umi CCTTCATGGC = 588 reads: +364 -8 +3 -1 +1 -1 +22 non-validated
umi CGATATGGCT = 1648 reads: -368X +4 -3XX +2 -1XX +2 -3XX +1 -1XX +2 -1XX +1 -1XX +2 -4XX +2 -2XX invalidated
umi CTGCTAAGGT = 331 reads: -366X +1 -2X +2 -2X +4 -1X +2 -3XX +1 -1XX +2 -3XX +2 -2XX +1 -1XX +2 -2XX invalidated
umi TCTTCCTCTG = 1156 reads: +400 validated

GOOD CONTIGS

TIG 1[bases=656]
210-553 ==> 0-343 on |643|TRBV5-6|L-REGION+V-REGION| [len=343] (mis=0)
556-604 ==> 0-48 on |537|TRBJ1-2|J-REGION| [len=48] (mis=4)
604-656 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 4 umis using 304 reads
cdr3 = CASSLETVYGYTF at 537, score = 8 + 9
umis assigned: [111, 247, 280, 351, 421, 425, 505, 649, 684, 794] and 4 others
of which 14 are surviving nonsolos
reads assigned: 15194
start codons at 76, 183, 210, 332, 352, 415, 496, 562
confident = true

TIG 2[bases=618]
0-176 ==> 0-176 on |496|TRAV3|5'UTR| [len=176] (mis=0)
176-519 ==> 0-343 on |497|TRAV3|L-REGION+V-REGION| [len=343] (mis=4)
517-576 ==> 0-59 on |421|TRAJ35|J-REGION| [len=59] (mis=1)
576-618 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 1 umis using 80 reads
cdr3 = CAVRGVGFGNVLHC at 503, score = 8 + 6
umis assigned: [62, 195, 385, 404, 500, 940]
of which 5 are surviving nonsolos
reads assigned: 4777
start codons at 176, 197, 206, 298, 339, 414, 531
confident = true
now this is a cell
paired!

CTTGTGAGCGACTCCGCTTTGTACTTCTGTGCTGTGAGGGGGGTAGGCTTTGGGAATGTGCTGCATTGCGGGTCCGGCACTCAAGTGATTGTTTTACCAC <==> GCCTTGTTGCTGGGGGACTCGGCCCTCTATCTCTGTGCCAGCAGCTTGGAGACAGTTTATGGCTACACCTTCGGTTCGGGGACCAGGTTAACCGTTGTAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.494 = CGTCCATAGGGTCTCC-1

using 73 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[73]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.495 = CGTCCATAGGTGACCA-1

using 82 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[81]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.496 = CGTCCATAGTACACCT-1

using 73 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[73]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.500 = CGTCCATAGTCAATAG-1

using 488 reads

====================================================================================

graph has 208 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 5[3, 5, 9, 16, 450]
surviving nonsolo ucounts = 1[450]
ids = [0]

====================================================================================

UMI info for barcode CGTCCATAGTCAATAG-1 contig 1 = TTCTTCCTGC...
umi ACCGGACCCC = 453 reads: +397 validated

GOOD CONTIGS

TIG 1[bases=562]
123-464 ==> 0-341 on |522|TRAV8-3|L-REGION+V-REGION| [len=341] (mis=4)
468-520 ==> 7-59 on |448|TRAJ7|J-REGION| [len=59] (mis=0)
520-562 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 1 umis using 17 reads
cdr3 = CAVVQGGNNRLAF at 450, score = 7 + 6
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 447
start codons at 123, 245, 259, 286, 433
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.505 = CGTCCATAGTCGTACT-1

using 137 reads

====================================================================================

graph has 96 edges initially, 10 edges after simplification

total ucounts = 7
nonsolo ucounts = 7[2, 4, 10, 18, 23, 26, 54]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.508 = CGTCCATAGTGACTCT-1

using 18 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.510 = CGTCCATAGTGGGTTG-1

using 67 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[67]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.513 = CGTCCATAGTTTCCTT-1

using 75 reads

====================================================================================

graph has 78 edges initially, 4 edges after simplification

total ucounts = 10
nonsolo ucounts = 5[3, 9, 14, 16, 28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.514 = CGTCCATCAAAGGTGC-1

using 20 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.522 = CGTCCATCAATTCCTT-1

using 80000 reads

====================================================================================

graph has 26793 edges initially, 460 edges after simplification

total ucounts = 2726
nonsolo ucounts = 1904[2^328, 3^244, 4^214, 5^137, 6^134, 7^113, 8^111, 9^80, 10^59, 11^67, 12^50, 13^46, 14^32, 15^24, 16^37, 17^15, 18^18, 19^14, 20^9, 21^12, 22^14, 23^8, 24^9, 25^9, 26^11, 27^5, 28^6, 29^2, 30^4, 31^3, 32^3, 33^4, 34^3, 35^5, 36^2, 37, 39^3, 40, 44^3, 48, 49^4, 50^3, 51, 52, 59, 65, 81, 111, 196, 210, 396, 511, 609, 622, 680, 715, 744, 767, 802, 807, 868, 871, 913, 946, 957, 988, 994, 1018, 1025, 1026, 1031, 1036, 1037, 1055, 1098, 1105, 1123, 1182, 1184, 1198, 1202, 1203, 1205, 1292, 1326, 1402, 1410, 1420, 1454, 1459, 1488, 1512, 2322, 2942, 3130, 3191, 3423, 5239]
surviving nonsolo ucounts = 47[210, 511, 622, 680, 715, 744, 767, 802, 807, 868, 871, 913, 946, 957, 988, 994, 1018, 1025, 1026, 1031, 1036, 1037, 1055, 1098, 1105, 1123, 1182, 1184, 1198, 1202, 1203, 1205, 1292, 1326, 1402, 1410, 1420, 1454, 1459, 1488, 1512, 2322, 2942, 3130, 3191, 3423, 5239]
ids = [2306, 162, 2698, 2395, 1018, 759, 733, 206, 2302, 1015, ...]

====================================================================================

UMI info for barcode CGTCCATCAATTCCTT-1 contig 1 = TGGGGGAGGT...
umi AAGTCATGCC = 1447 reads: +400 validated
umi AATTAATTCG = 937 reads: +400 validated
umi ACAATACACC = 1520 reads: +400 validated
umi ACAGCCCCCT = 469 reads: -325X +1 -1X +2 -3X +2 -8XX +1 -6XX +1 -2XX +1 -1XX +1 -1XX +44 invalidated
umi ACCCACGGCG = 830 reads: +400 validated
umi ACCGTCGGGC = 1117 reads: -365X +1 -2XX +6 -3XX +3 -1XX +2 -1XX +1 -2XX +5 -2XX +2 -1XX +1 -1XX +1 invalidated
umi ACTTAGACTC = 1300 reads: +400 validated
umi AGACGCTGCG = 1116 reads: +400 validated
umi CAAAGGGCCG = 1407 reads: +400 validated
umi CAACAGTCGG = 996 reads: +400 validated
umi CAATATCGTA = 960 reads: +400 validated
umi CACGCTATCA = 872 reads: +400 validated
umi CATCATGTCG = 1020 reads: +400 validated
umi CATGAGTTAA = 753 reads: +400 validated
umi CATTCATCTC = 740 reads: +400 validated
umi CCTCGCTATT = 2799 reads: -363 +1 -1XX +1 -2XX +6 -3XX +3 -1XX +2 -1XX +1 -2XX +5 -2XX +2 -1XX +1 -1XX +1 invalidated
umi CCTTATTGTA = 1026 reads: +400 validated
umi CGACTCTCAC = 1038 reads: +400 validated
umi CGCATCTCCG = 863 reads: +400 validated
umi CGCCAGCGTT = 715 reads: +400 validated
umi CTGTGTTGCG = 1176 reads: +400 validated
umi CTGTTACTCT = 2945 reads: -157X +2 -1XX +240 invalidated
umi GAACCAACCG = 985 reads: +400 validated
umi GATGGCCATT = 1485 reads: +400 validated
umi GTATACCTTT = 1416 reads: +400 validated
umi GTTTGGTGGG = 1044 reads: +400 validated
umi TGACTCTTCC = 803 reads: +400 validated
umi TGTAGCTCTT = 675 reads: +400 validated
umi TTACGCTCGA = 1031 reads: +400 validated
umi TTCGGCCGGC = 1109 reads: +400 validated
umi TTTTATTTGT = 619 reads: +400 validated

UMI info for barcode CGTCCATCAATTCCTT-1 contig 2 = TGGGGGCATT...
umi ACCAGTGAGC = 1429 reads: -6 +1 -1X +1 -1X +390 invalidated
umi ATGTGCTTCC = 1458 reads: +400 validated
umi CGGCGACAAC = 5270 reads: -175X +225 invalidated
umi GACCGGAACA = 1206 reads: +400 validated
umi GCCTTCGGGT = 1191 reads: -8X +392 invalidated
umi GGACGGTCAA = 1104 reads: +400 validated
umi GGATGCGATC = 1023 reads: +400 validated
umi GGTTCCCACA = 1008 reads: +400 validated
umi TAGTTTAGCA = 1333 reads: -8X +392 invalidated
umi TATTATAGCA = 1210 reads: +400 validated
umi TGAGCGCGTT = 212 reads: +377 -1 +1 -1 +20 non-validated
umi TTCACGTGTC = 110 reads: -377X +1 -9X +1 -3XX +1 -2XX +1 -5XX invalidated
umi TTTCTTCGGG = 918 reads: +400 validated

GOOD CONTIGS

TIG 1[bases=487]
35-378 ==> 0-343 on |639|TRBV5-5|L-REGION+V-REGION| [len=343] (mis=2)
377-389 ==> 0-12 on |535|TRBD1|D-REGION| [len=12] (mis=2)
385-435 ==> 0-50 on |540|TRBJ1-5|J-REGION| [len=50] (mis=4)
435-487 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 28 umis using 2211 reads
cdr3 = CASSLGAGGSQPQHF at 362, score = 8 + 8
umis assigned: [100, 132, 147, 162, 206, 220, 292, 315, 585, 590] and 21 others
of which 31 are surviving nonsolos
reads assigned: 34672
start codons at 35, 157, 240, 321, 411
confident = true

TIG 2[bases=458]
16-354 ==> 0-338 on |477|TRAV2|L-REGION+V-REGION| [len=338] (mis=0)
354-416 ==> 0-62 on |446|TRAJ6|J-REGION| [len=62] (mis=5)
416-458 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 12 umis using 1363 reads
cdr3 = CAVEDPARGSYIPTF at 340, score = 7 + 7
umis assigned: [202, 524, 1056, 1397, 1537, 1625, 1637, 1715, 1998, 2043] and 3 others
of which 12 are surviving nonsolos
reads assigned: 17189
start codons at 16, 173, 210, 268, 275, 323
confident = true

REJECT CONTIGS

TIG 1[bases=400]
5-140 ==> 2433-2568 on segment before TRBJ2-1 exon 1 [len=3226] (mis=0)
196-298 ==> 0-102 on segment before TRBJ2-4 exon 1 [len=102] (mis=0)
298-348 ==> 0-50 on |547|TRBJ2-4|J-REGION| [len=50] (mis=0)
348-400 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
umis assigned: [20, 181, 1099, 1553, 1978]
of which 4 are surviving nonsolos
reads assigned: 9881
start codons at 122
confident = false
did not find CDR3
now this is a cell
paired!

GAGGCAGATGCTGCTGTTTACTACTGTGCTGTGGAGGATCCTGCCAGAGGAAGCTACATACCTACATTTGGAAGAGGAACCAGCCTTATTGTTCATCCGT <==> TTGCTGGGGGACTCGGCCCTGTATCTCTGTGCCAGCAGCTTAGGGGCAGGGGGGAGTCAGCCCCAGCATTTTGGTGATGGGACTCGACTCTCCATCCTAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.523 = CGTCCATCACACATGT-1

using 30 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.525 = CGTCCATCACAGGCCT-1

using 46 reads

====================================================================================

graph has 54 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 4[2, 3, 10, 30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.532 = CGTCCATCACGAAAGC-1

using 22 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.538 = CGTCCATCACTTGGAT-1

using 32 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[2, 3, 25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.540 = CGTCCATCAGACAAGC-1

using 115 reads

====================================================================================

graph has 98 edges initially, 4 edges after simplification

total ucounts = 7
nonsolo ucounts = 5[5, 9, 10, 29, 60]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.542 = CGTCCATCAGCCTTTC-1

using 26 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.548 = CGTCCATCATACCATG-1

using 79 reads

====================================================================================

graph has 60 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[79]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.549 = CGTCCATCATACGCTA-1

using 41 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 4[3, 5, 8, 24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.550 = CGTCCATCATACTACG-1

using 57 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[57]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.553 = CGTCCATCATGTAGTC-1

using 29 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.554 = CGTCCATCATGTCCTC-1

using 47 reads

====================================================================================

graph has 68 edges initially, 4 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[47]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.556 = CGTCCATCATTCTTAC-1

using 960 reads

====================================================================================

graph has 330 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 4[3, 6, 18, 928]
surviving nonsolo ucounts = 1[928]
ids = [5]

====================================================================================

REJECT CONTIGS

TIG 1[bases=500]
0-112 ==> 0-112 on |618|TRBV30|5'UTR| [len=112] (mis=0)
112-447 ==> 0-335 on |619|TRBV30|L-REGION+V-REGION| [len=335] (mis=1)
452-500 ==> 0-48 on |536|TRBJ1-1|J-REGION| [len=48] (mis=5)
cdr3 = CAWSGTVNTEAFF at 433, score = 7 + 5
umis assigned: [5]
of which 1 are surviving nonsolos
reads assigned: 843
start codons at 109, 112, 180, 477
confident = false
not full
full length stopped transcript of length 500
frameshifted full length stopped transcript of length 500
VJ delta = 14
not full
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.558 = CGTCCATCATTTCACT-1

using 81 reads

====================================================================================

graph has 66 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 3[6, 23, 49]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.559 = CGTCCATCATTTGCCC-1

using 44 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[44]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.563 = CGTCCATGTAATCGTC-1

using 397 reads

====================================================================================

graph has 347 edges initially, 14 edges after simplification

total ucounts = 38
nonsolo ucounts = 29[2^3, 3^6, 5^3, 7^3, 8, 11, 13, 17, 19, 21, 24^3, 27, 30, 36^2, 38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.565 = CGTCCATGTACTCTCC-1

using 54 reads

====================================================================================

graph has 48 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[54]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.567 = CGTCCATGTATTCGTG-1

using 53 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 5[6^2, 7, 14, 19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.569 = CGTCCATGTCAACTGT-1

using 64 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[64]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.571 = CGTCCATGTCACCCAG-1

using 43 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[43]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.576 = CGTCCATGTCCGAGTC-1

using 339 reads

====================================================================================

graph has 276 edges initially, 10 edges after simplification

total ucounts = 79
nonsolo ucounts = 47[2^12, 3^11, 4^6, 5^4, 6^3, 7^3, 9^2, 12, 22^2, 27, 32, 34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.580 = CGTCCATGTCGAAAGC-1

using 54 reads

====================================================================================

graph has 64 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[2, 11, 12, 27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.582 = CGTCCATGTCGCGTGT-1

using 6751 reads

====================================================================================

graph has 3599 edges initially, 112 edges after simplification

total ucounts = 640
nonsolo ucounts = 438[2^102, 3^69, 4^30, 5^46, 6^29, 7^21, 8^20, 9^15, 10^10, 11^15, 12^7, 13^7, 14^5, 15^4, 16^4, 17^3, 18^3, 19^3, 20^4, 21^3, 22, 23^2, 25, 26^2, 27^3, 28, 29, 32, 33, 34^3, 35, 36^2, 38, 39, 40, 41^2, 43, 44, 46, 48, 52, 54, 61, 94, 210, 372, 377, 599, 690, 787]
surviving nonsolo ucounts = 7[38, 210, 372, 377, 599, 690, 787]
ids = [151, 46, 475, 371, 539, 532, 355]

====================================================================================

UMI info for barcode CGTCCATGTCGCGTGT-1 contig 1 = GGGGAGGACA...
umi AAGTGCTGCT = 212 reads: +382 -15 +6 non-validated
umi GACACCATCC = 792 reads: +403 validated
umi GATAATTCCC = 337 reads: -381 +5 -2XX +7 -1XX +3 -2XX +2 invalidated
umi TCTCTGCGTC = 596 reads: +403 validated
umi TTGATAATGG = 1 reads: -398 +1 -1X +1 -2X invalidated

UMI info for barcode CGTCCATGTCGCGTGT-1 contig 2 = CCCACATGAA...
umi TAGATTGCCG = 232 reads: +345 -26 +14 non-validated
umi TCTAACTGTC = 692 reads: +385 validated

GOOD CONTIGS

TIG 1[bases=587]
132-475 ==> 0-343 on |643|TRBV5-6|L-REGION+V-REGION| [len=343] (mis=4)
482-535 ==> 0-53 on |542|TRBJ1-6|J-REGION| [len=53] (mis=0)
535-587 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 2 umis using 109 reads
cdr3 = CASSERSGSYNSPLHF at 459, score = 8 + 9
umis assigned: [46, 355, 371, 539, 608]
of which 4 are surviving nonsolos
reads assigned: 1917
start codons at 105, 132, 254, 274, 337, 418, 511
confident = true

TIG 2[bases=511]
0-84 ==> 0-84 on |453|TRAV1-2|5'UTR| [len=84] (mis=0)
84-402 ==> 0-318 on |454|TRAV1-2|L-REGION+V-REGION| [len=318] (mis=1)
406-469 ==> 0-63 on |426|TRAJ4|J-REGION| [len=63] (mis=2)
469-511 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 1 umis using 39 reads
cdr3 = CATTFSGGYNKLIF at 396, score = 7 + 9
umis assigned: [475, 532]
of which 2 are surviving nonsolos
reads assigned: 911
start codons at 5, 33, 51, 84, 106, 114, 120, 162, 247, 280, 289, 372
confident = true
now this is a cell
paired!

CAGATGAAAGACTCTGCCTCTTACCTCTGTGCTACCACCTTTTCTGGTGGCTACAATAAGCTGATTTTTGGAGCAGGGACCAGGCTGGCTGTACACCCAT <==> CTGGGGGACTCGGCCCTCTATCTCTGTGCCAGCAGCGAAAGATCCGGCTCCTATAATTCACCCCTCCACTTTGGGAATGGGACCAGGCTCACTGTGACAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.587 = CGTCCATGTCTAGTCA-1

using 285 reads

====================================================================================

graph has 233 edges initially, 6 edges after simplification

total ucounts = 16
nonsolo ucounts = 13[2, 3, 5, 10, 11, 12, 18, 20, 30, 31, 39, 49, 52]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.603 = CGTCCATGTTCCCTTG-1

using 25 reads

====================================================================================

graph has 31 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.604 = CGTCCATGTTCTCATT-1

using 29 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.613 = CGTCCATTCAACGAAA-1

using 51 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 4[2, 3^2, 39]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.615 = CGTCCATTCAAGATCC-1

using 58 reads

====================================================================================

graph has 80 edges initially, 2 edges after simplification

total ucounts = 12
nonsolo ucounts = 6[3, 7, 8, 10, 11, 13]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.619 = CGTCCATTCAGAGGTG-1

using 38 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 4[2, 5, 14^2]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.621 = CGTCCATTCAGGATCT-1

using 126 reads

====================================================================================

graph has 126 edges initially, 2 edges after simplification

total ucounts = 22
nonsolo ucounts = 12[2^5, 3^2, 7, 16, 21, 24, 32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.622 = CGTCCATTCATCACCC-1

using 76 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[76]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.629 = CGTCCATTCCCTCTTT-1

using 32 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 5[3, 5^2, 7^2]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.633 = CGTCCATTCCTAGTGA-1

using 21 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.634 = CGTCCATTCCTGCCAT-1

using 18338 reads

====================================================================================

graph has 8728 edges initially, 194 edges after simplification

total ucounts = 1310
nonsolo ucounts = 960[2^222, 3^134, 4^102, 5^61, 6^65, 7^52, 8^47, 9^19, 10^24, 11^26, 12^18, 13^22, 14^18, 15^8, 16^15, 17^9, 18^12, 19^7, 20^8, 21^4, 22^7, 23^6, 24^5, 25^7, 26^4, 27^5, 28, 29^2, 30^2, 31^2, 32, 33, 35^5, 36, 37^4, 38^2, 39^2, 40, 41^2, 42^3, 44^2, 45^4, 49, 50, 52, 54, 55, 56, 58, 63, 65, 66, 77, 81^2, 94, 132, 1275, 4149, 4157]
surviving nonsolo ucounts = 3[1275, 4149, 4157]
ids = [1036, 661, 177]

====================================================================================

UMI info for barcode CGTCCATTCCTGCCAT-1 contig 1 = AGAGGCCCCA...
umi TCATTCGGGC = 1264 reads: +406 validated

GOOD CONTIGS

TIG 1[bases=467]
0-29 ==> 0-29 on |622|TRBV4-1|5'UTR| [len=29] (mis=0)
29-373 ==> 0-344 on |623|TRBV4-1|L-REGION+V-REGION| [len=344] (mis=0)
377-389 ==> 0-12 on |535|TRBD1|D-REGION| [len=12] (mis=0)
394-435 ==> 7-48 on |537|TRBJ1-2|J-REGION| [len=48] (mis=0)
435-467 ==> 0-32 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 1 umis using 116 reads
cdr3 = CASSQDLGTGGAGGYTF at 356, score = 10 + 9
umis assigned: [1036]
of which 1 are surviving nonsolos
reads assigned: 1254
start codons at 29, 122, 128, 151, 164, 179, 221, 237, 255, 286
confident = false

REJECT CONTIGS

TIG 1[bases=311]
5-60 ==> 31-86 on segment before TRBJ2-2P exon 1 [len=86] (mis=0)
60-106 ==> 0-46 on |545|TRBJ2-2P|J-REGION| [len=46] (mis=0)
106-210 ==> 0-104 on segment before TRBJ2-3 exon 1 [len=104] (mis=0)
259-311 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
umis assigned: [177, 661]
of which 2 are surviving nonsolos
reads assigned: 8201
start codons at 17, 120
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.636 = CGTCCATTCGCCGTGA-1

using 23 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[6, 15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.637 = CGTCCATTCGCCTGAG-1

using 81 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[81]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.638 = CGTCCATTCGCGTTTC-1

using 807 reads

====================================================================================

graph has 439 edges initially, 18 edges after simplification

total ucounts = 30
nonsolo ucounts = 27[2^5, 5, 6, 9^3, 11, 14^2, 16, 19, 20, 23, 30, 33, 58, 60, 61, 67, 70, 79, 89, 92]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.639 = CGTCCATTCGCTTGTC-1

using 15 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.640 = CGTCCATTCGGCGGTT-1

using 210 reads

====================================================================================

graph has 166 edges initially, 10 edges after simplification

total ucounts = 18
nonsolo ucounts = 14[2, 3^3, 4, 6, 8, 9^2, 11, 13, 36, 49, 50]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.641 = CGTCCATTCGTCCGTT-1

using 34 reads

====================================================================================

graph has 48 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.642 = CGTCCATTCGTCTGCT-1

using 32 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.643 = CGTCCATTCGTTACGA-1

using 48 reads

====================================================================================

graph has 6 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 1[46]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.645 = CGTCCATTCTCCCTGA-1

using 97 reads

====================================================================================

graph has 130 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 9[2^2, 3, 4, 5, 7, 9, 22, 42]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.647 = CGTCCATTCTGAAAGA-1

using 60 reads

====================================================================================

graph has 42 edges initially, 4 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[30^2]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.652 = CGTCCATTCTGGCGAC-1

using 351 reads

====================================================================================

graph has 322 edges initially, 14 edges after simplification

total ucounts = 22
nonsolo ucounts = 15[3, 5, 6, 8, 10^2, 12, 13, 15, 20, 22, 29, 53, 59, 79]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.653 = CGTCCATTCTGTCCGT-1

using 34 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.654 = CGTCCATTCTGTCTCG-1

using 13819 reads

====================================================================================

graph has 7151 edges initially, 148 edges after simplification

total ucounts = 1152
nonsolo ucounts = 855[2^185, 3^141, 4^86, 5^72, 6^49, 7^39, 8^34, 9^15, 10^29, 11^24, 12^22, 13^10, 14^15, 15^17, 16^8, 17^7, 18^6, 19^10, 20^4, 21^5, 22^10, 23^5, 24^4, 25^7, 26^5, 27^2, 28^3, 29^4, 30^2, 31^3, 32, 33^4, 34, 36^4, 37^3, 38^2, 39^2, 40^2, 41, 42^3, 44, 47, 51, 53, 57, 59, 119, 1754, 4727]
surviving nonsolo ucounts = 2[1754, 4727]
ids = [223, 763]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.656 = CGTCCATTCTTATCTG-1

using 78 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[19, 59]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.657 = CGTCCATTCTTCAACT-1

using 16 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[16]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.659 = CGTCCATTCTTGCAAG-1

using 40 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 12
nonsolo ucounts = 8[2, 3^3, 4^2, 8, 9]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.662 = CGTCCATTCTTTCCTC-1

using 18 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.665 = CGTCTACAGAAGGGTA-1

using 161 reads

====================================================================================

graph has 104 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 6[2, 3, 6, 9, 16, 123]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.666 = CGTCTACAGAATGTGT-1

using 24 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.667 = CGTCTACAGACTAAGT-1

using 23 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.669 = CGTCTACAGAGACTAT-1

using 39 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[2, 6, 29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.670 = CGTCTACAGAGAGCTC-1

using 842 reads

====================================================================================

graph has 326 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 5[2, 4, 9, 25, 800]
surviving nonsolo ucounts = 1[800]
ids = [0]

====================================================================================

UMI info for barcode CGTCTACAGAGAGCTC-1 contig 1 = CCCACATGAA...
umi AAGCACGAAA = 790 reads: +391 validated

GOOD CONTIGS

TIG 1[bases=517]
0-84 ==> 0-84 on |453|TRAV1-2|5'UTR| [len=84] (mis=0)
84-402 ==> 0-318 on |454|TRAV1-2|L-REGION+V-REGION| [len=318] (mis=1)
409-475 ==> 0-66 on |402|TRAJ18|J-REGION| [len=66] (mis=1)
475-517 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 1 umis using 21 reads
cdr3 = CAATSDRGSTLGRLYF at 396, score = 7 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 778
start codons at 5, 33, 51, 84, 106, 114, 120, 162, 247, 280, 289, 372
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.677 = CGTCTACAGATCTGAA-1

using 52 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[52]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.678 = CGTCTACAGATGGCGT-1

using 36 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[2, 6, 27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.680 = CGTCTACAGATGTAAC-1

using 92 reads

====================================================================================

graph has 74 edges initially, 4 edges after simplification

total ucounts = 9
nonsolo ucounts = 6[3, 4, 14, 18, 25^2]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.681 = CGTCTACAGCAATATG-1

using 958 reads

====================================================================================

graph has 380 edges initially, 6 edges after simplification

total ucounts = 13
nonsolo ucounts = 9[3, 5^3, 9, 14, 46, 48, 819]
surviving nonsolo ucounts = 1[819]
ids = [8]

====================================================================================

UMI info for barcode CGTCTACAGCAATATG-1 contig 1 = AGATCAGAAG...
umi TCAACTCTCT = 820 reads: +403 validated

GOOD CONTIGS

TIG 1[bases=491]
0-46 ==> 0-46 on |506|TRAV38-2/DV8|5'UTR| [len=46] (mis=0)
46-395 ==> 0-349 on |507|TRAV38-2/DV8|L-REGION+V-REGION| [len=349] (mis=4)
393-449 ==> 0-56 on |436|TRAJ49|J-REGION| [len=56] (mis=3)
449-491 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 1 umis using 35 reads
cdr3 = CAYGKLTGNQFYF at 379, score = 7 + 7
umis assigned: [8]
of which 1 are surviving nonsolos
reads assigned: 808
start codons at 46, 51, 103, 136, 179, 238, 278, 362, 370, 386
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.683 = CGTCTACAGCCACTAT-1

using 14 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[14]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.687 = CGTCTACAGCTCCCAG-1

using 61 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[61]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.692 = CGTCTACAGGCTAGCA-1

using 28 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.698 = CGTCTACAGTAACCCT-1

using 35 reads

====================================================================================

graph has 82 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 4[2^2, 5, 25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.701 = CGTCTACAGTATCTCG-1

using 89 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[89]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.702 = CGTCTACAGTCAAGCG-1

using 48 reads

====================================================================================

graph has 56 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 3[7, 16, 22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.703 = CGTCTACAGTCCATAC-1

using 105 reads

====================================================================================

graph has 78 edges initially, 4 edges after simplification

total ucounts = 15
nonsolo ucounts = 10[2^3, 3, 7^2, 9, 11, 20, 37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.704 = CGTCTACAGTCCCACG-1

using 42 reads

====================================================================================

graph has 54 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 4[2, 4, 5, 30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.707 = CGTCTACAGTGATCGG-1

using 39 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 4[2^3, 30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.712 = CGTCTACAGTTGAGAT-1

using 266 reads

====================================================================================

graph has 156 edges initially, 8 edges after simplification

total ucounts = 13
nonsolo ucounts = 13[2, 3^2, 5, 9, 10, 11, 13, 24, 26, 28, 41, 91]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.715 = CGTCTACCAACTGCGC-1

using 87 reads

====================================================================================

graph has 48 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[87]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.717 = CGTCTACCAAGAGGCT-1

using 49 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[49]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.719 = CGTCTACCAAGCGAGT-1

using 30 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.720 = CGTCTACCAAGTTGTC-1

using 31 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.722 = CGTCTACCAATCCAAC-1

using 93 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[92]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.726 = CGTCTACCAATGTTGC-1

using 84 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[84]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.727 = CGTCTACCACAAGACG-1

using 42 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[42]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.728 = CGTCTACCACACTGCG-1

using 43 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[43]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.729 = CGTCTACCACAGACAG-1

using 33 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 2[4, 26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.731 = CGTCTACCACATCCGG-1

using 94 reads

====================================================================================

graph has 104 edges initially, 4 edges after simplification

total ucounts = 14
nonsolo ucounts = 9[2^3, 4, 9, 11, 16^2, 27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.732 = CGTCTACCACATGACT-1

using 86 reads

====================================================================================

graph has 48 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[15, 71]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.733 = CGTCTACCACCATCCT-1

using 66 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[66]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.739 = CGTCTACCACTGTCGG-1

using 4775 reads

====================================================================================

graph has 3770 edges initially, 114 edges after simplification

total ucounts = 357
nonsolo ucounts = 257[2^48, 3^32, 4^24, 5^22, 6^12, 7^8, 8^7, 9^14, 10^9, 11^3, 12^9, 13^2, 14^5, 15^9, 16^3, 17^4, 18, 19^4, 21^2, 22^4, 23, 24, 25^2, 26^2, 27^3, 28, 29, 30, 32^2, 36^3, 38^2, 40, 43, 45^2, 46, 51^2, 52, 70, 76, 79, 83, 183, 303, 513, 920]
surviving nonsolo ucounts = 3[303, 513, 920]
ids = [100, 125, 293]

====================================================================================

REJECT CONTIGS

TIG 1[bases=504]
0-47 ==> 0-47 on |674|TRBV7-2|5'UTR| [len=47] (mis=0)
39-60 ==> 1110-1131 on segment before TRBV6-2 exon 1 [len=3166] (mis=1)
47-394 ==> 0-347 on |675|TRBV7-2|L-REGION+V-REGION| [len=347] (mis=3)
404-452 ==> 0-48 on |536|TRBJ1-1|J-REGION| [len=48] (mis=4)
452-504 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
cdr3 = CASSLVTS at 377, score = 8 + 5
umis assigned: [100, 190, 293]
of which 2 are surviving nonsolos
reads assigned: 1241
start codons at 47, 156
confident = false
not full
frameshifted full length transcript of length 504
VJ delta = -6
not full

TIG 2[bases=588]
1-77 ==> 49-125 on segment before TRBV15 exon 2 [len=125] (mis=0)
73-372 ==> 45-344 on |579|TRBV15|L-REGION+V-REGION| [len=344] (mis=4)
531-582 ==> 0-51 on |539|TRBJ1-4|J-REGION| [len=51] (mis=0)
cdr3 = CATSAGRDAKTIYF at 355, score = 6 + 7
umis assigned: [125]
of which 1 are surviving nonsolos
reads assigned: 490
start codons at 80, 86, 91, 178, 233, 251, 346, 523, 537
confident = false
not full
VJ delta = -137
delta too large
not full
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.742 = CGTCTACCACTTGGAT-1

using 18 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 1[15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.743 = CGTCTACCAGACAAAT-1

using 65 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[65]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.745 = CGTCTACCAGACGCTC-1

using 162 reads

====================================================================================

graph has 132 edges initially, 4 edges after simplification

total ucounts = 10
nonsolo ucounts = 10[2^2, 6, 8, 13, 14, 20, 27, 29, 41]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.748 = CGTCTACCAGCTCGCA-1

using 21 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.750 = CGTCTACCAGGGATTG-1

using 68 reads

====================================================================================

graph has 78 edges initially, 4 edges after simplification

total ucounts = 10
nonsolo ucounts = 6[2^2, 5, 12, 13, 30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.755 = CGTCTACCATCGGAAG-1

using 27 reads

====================================================================================

graph has 35 edges initially, 5 edges after simplification

total ucounts = 3
nonsolo ucounts = 1[25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.759 = CGTCTACCATGTCCTC-1

using 110 reads

====================================================================================

graph has 112 edges initially, 4 edges after simplification

total ucounts = 7
nonsolo ucounts = 6[2, 3, 8, 21, 25, 50]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.762 = CGTCTACCATTCTCAT-1

using 40 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[11, 12, 16]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.769 = CGTCTACGTCACCCAG-1

using 53 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[53]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.773 = CGTCTACGTCGCGTGT-1

using 81 reads

====================================================================================

graph has 96 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 6[3, 4, 7, 9, 16, 39]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.775 = CGTCTACGTCGTCTTC-1

using 250 reads

====================================================================================

graph has 152 edges initially, 8 edges after simplification

total ucounts = 15
nonsolo ucounts = 13[2^4, 6, 8^2, 21, 24, 34, 41, 44, 54]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.782 = CGTCTACGTGATGTCT-1

using 54 reads

====================================================================================

graph has 39 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[53]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.785 = CGTCTACGTGGCTCCA-1

using 29 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.787 = CGTCTACGTGTCTGAT-1

using 112 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[112]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.789 = CGTCTACGTGTTTGGT-1

using 51 reads

====================================================================================

graph has 66 edges initially, 2 edges after simplification

total ucounts = 12
nonsolo ucounts = 6[2^2, 4, 5, 10, 22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.795 = CGTCTACTCAAAGACA-1

using 56 reads

====================================================================================

graph has 66 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 7[2, 3, 5, 7^2, 10, 20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.797 = CGTCTACTCAAGATCC-1

using 43 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[43]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.798 = CGTCTACTCAAGGCTT-1

using 1756 reads

====================================================================================

graph has 564 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 2[672, 1081]
surviving nonsolo ucounts = 2[672, 1081]
ids = [0, 3]

====================================================================================

UMI info for barcode CGTCTACTCAAGGCTT-1 contig 1 = TTTGCCCTGC...
umi AGCACACTAC = 666 reads: +406 validated
umi CTTTCTTCGC = 1072 reads: +406 validated

GOOD CONTIGS

TIG 1[bases=498]
0-40 ==> 41-81 on |585|TRBV18|5'UTR| [len=81] (mis=0)
40-387 ==> 0-347 on |586|TRBV18|L-REGION+V-REGION| [len=347] (mis=3)
384-400 ==> 0-16 on |730|TRBD2|D-REGION| [len=16] (mis=3)
397-446 ==> 0-49 on |546|TRBJ2-3|J-REGION| [len=49] (mis=4)
446-498 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 2 umis using 84 reads
cdr3 = CASSPGLAGDADTQYF at 370, score = 7 + 8
umis assigned: [0, 3]
of which 2 are surviving nonsolos
reads assigned: 1720
start codons at 25, 40, 98, 109, 162, 172, 188, 232, 263, 274, 398
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.800 = CGTCTACTCAATACCG-1

using 50 reads

====================================================================================

graph has 48 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 2[13, 34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.802 = CGTCTACTCACAACGT-1

using 77 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[77]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.805 = CGTCTACTCAGGATCT-1

using 55 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[55]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.806 = CGTCTACTCAGGCAAG-1

using 110 reads

====================================================================================

graph has 84 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 7[2^2, 4, 5, 7^2, 79]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.807 = CGTCTACTCAGTCAGT-1

using 274 reads

====================================================================================

graph has 145 edges initially, 8 edges after simplification

total ucounts = 12
nonsolo ucounts = 12[2, 3^2, 5, 6, 10, 12, 28, 35, 38, 51, 81]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.810 = CGTCTACTCATACGGT-1

using 10 reads

====================================================================================

graph has 4 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[10]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.813 = CGTCTACTCCAAAGTC-1

using 26 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.814 = CGTCTACTCCAAATGC-1

using 117 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[117]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.815 = CGTCTACTCCAATGGT-1

using 25 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.817 = CGTCTACTCCCTAATT-1

using 24 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.820 = CGTCTACTCCGTACAA-1

using 1176 reads

====================================================================================

graph has 468 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 5[2^2, 5, 7, 1154]
surviving nonsolo ucounts = 1[1154]
ids = [5]

====================================================================================

UMI info for barcode CGTCTACTCCGTACAA-1 contig 1 = GTTCCCCTAT...
umi CTGGATGCCT = 1147 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=503]
0-57 ==> 0-57 on |647|TRBV6-1|5'UTR| [len=57] (mis=0)
57-401 ==> 0-344 on |648|TRBV6-1|L-REGION+V-REGION| [len=344] (mis=3)
401-451 ==> 0-50 on |538|TRBJ1-3|J-REGION| [len=50] (mis=5)
451-503 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 1 umis using 63 reads
cdr3 = CASSARRTNTIYF at 384, score = 9 + 7
umis assigned: [5]
of which 1 are surviving nonsolos
reads assigned: 1130
start codons at 15, 57, 115, 168, 192, 207, 234, 298, 307
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.824 = CGTCTACTCCTCATTA-1

using 43 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[43]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.825 = CGTCTACTCCTTAATC-1

using 56 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[7, 47]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.826 = CGTCTACTCGACCAGC-1

using 76 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[76]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.833 = CGTCTACTCGTAGGAG-1

using 105 reads

====================================================================================

graph has 118 edges initially, 4 edges after simplification

total ucounts = 10
nonsolo ucounts = 8[2, 3, 6, 7, 9, 13, 23, 40]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.835 = CGTCTACTCGTTGACA-1

using 64 reads

====================================================================================

graph has 68 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[64]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.838 = CGTCTACTCTCACATT-1

using 29 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.844 = CGTCTACTCTGCTTGC-1

using 34 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.846 = CGTCTACTCTTCGAGA-1

using 38 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.848 = CGTGAGCAGAACAACT-1

using 15 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.849 = CGTGAGCAGAAGATTC-1

using 64 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 4[2, 5, 9, 47]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.854 = CGTGAGCAGAGCTGCA-1

using 73 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[72]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.856 = CGTGAGCAGATGTGTA-1

using 80 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[7, 71]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.858 = CGTGAGCAGCCACCTG-1

using 41 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[9, 32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.862 = CGTGAGCAGCCGGTAA-1

using 5875 reads

====================================================================================

graph has 3654 edges initially, 158 edges after simplification

total ucounts = 435
nonsolo ucounts = 307[2^44, 3^23, 4^18, 5^18, 6^18, 7^17, 8^15, 9^8, 10, 11^4, 12^6, 13^10, 14^2, 15^7, 16^7, 17^3, 18^4, 19, 20, 21^2, 22^2, 23^2, 24^6, 25, 27, 28^4, 29^4, 30^3, 31^2, 32^2, 33, 34^4, 35^4, 36^2, 37^2, 38^5, 39^2, 40^2, 41, 42^3, 43^3, 44^4, 45^4, 46, 47^2, 48, 50, 53^3, 54^2, 55^3, 57, 59, 60^2, 61, 62, 63, 64^2, 65^2, 66, 69, 71^2, 74, 76^2, 82, 83, 101]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.863 = CGTGAGCAGCCTATGT-1

using 92 reads

====================================================================================

graph has 78 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[3, 8, 80]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.868 = CGTGAGCAGGATCGCA-1

using 27 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[12, 14]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.871 = CGTGAGCAGGGCTTGA-1

using 315 reads

====================================================================================

graph has 302 edges initially, 14 edges after simplification

total ucounts = 27
nonsolo ucounts = 19[2^3, 3^3, 5, 6, 7^2, 9, 16, 17, 21, 22, 29, 45, 46, 62]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.874 = CGTGAGCAGTCTCCTC-1

using 154 reads

====================================================================================

graph has 86 edges initially, 4 edges after simplification

total ucounts = 5
nonsolo ucounts = 4[4, 22, 34, 93]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.875 = CGTGAGCAGTCTTGCA-1

using 34 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[5, 28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.876 = CGTGAGCAGTGAACGC-1

using 27 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.877 = CGTGAGCAGTGACATA-1

using 237 reads

====================================================================================

graph has 176 edges initially, 6 edges after simplification

total ucounts = 17
nonsolo ucounts = 14[2, 3^2, 4, 5^3, 8, 9, 14, 21, 29, 53, 73]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.878 = CGTGAGCAGTGGCACA-1

using 33 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.880 = CGTGAGCAGTTGAGTA-1

using 31 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[8, 21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.882 = CGTGAGCCAAACAACA-1

using 15297 reads

====================================================================================

graph has 6554 edges initially, 72 edges after simplification

total ucounts = 582
nonsolo ucounts = 366[2^83, 3^58, 4^45, 5^36, 6^26, 7^22, 8^11, 9^18, 10^9, 11^3, 12^3, 13, 14^2, 15^4, 16^3, 18, 20, 22^2, 23^2, 26, 28^2, 30, 31, 34^2, 38^3, 47^2, 49, 53, 57, 63, 70, 82, 91, 103, 227, 261, 312, 427, 495, 523, 539, 705, 927, 990, 995, 1008, 1122, 1253, 1266, 1348]
surviving nonsolo ucounts = 15[261, 312, 427, 495, 523, 539, 705, 927, 990, 995, 1008, 1122, 1253, 1266, 1348]
ids = [200, 420, 429, 280, 553, 164, 195, 215, 109, 516, ...]

====================================================================================

UMI info for barcode CGTGAGCCAAACAACA-1 contig 1 = GAGCACAGAC...
umi ATCCTAACAC = 987 reads: +397 validated
umi CACAACTTCT = 1271 reads: +397 validated
umi CCATATCGAC = 711 reads: +397 validated
umi CCTATAGTTA = 922 reads: +397 validated
umi CCTCCACGGA = 201 reads: -395X +2 invalidated
umi GTCACACACC = 1348 reads: +397 validated
umi TATCACCACT = 1258 reads: +397 validated
umi TGCGCATCAT = 994 reads: +397 validated
umi TTGGCATCCT = 1016 reads: +397 validated

UMI info for barcode CGTGAGCCAAACAACA-1 contig 2 = GGGTAAAACT...
umi AATGGACCCT = 1129 reads: +403 validated
umi CACATTGATC = 550 reads: +403 validated
umi CCCACTAGGC = 265 reads: +403 validated
umi CTCAATGGCA = 503 reads: +246 -1 +1 -3X +3 -3XX +2 -1XX +143 invalidated
umi GTTAGGCTTC = 316 reads: +374 -2 +27 non-validated
umi TAAATGGGAT = 60 reads: -388X +3 -1XX +3 -4XX +3 -1XX invalidated
umi TTCTACTATC = 86 reads: -388X +3 -1XX +3 -4XX +3 -1XX invalidated

GOOD CONTIGS

TIG 1[bases=484]
0-35 ==> 274-309 on |630|TRBV5-1|5'UTR| [len=309] (mis=0)
35-378 ==> 0-343 on |631|TRBV5-1|L-REGION+V-REGION| [len=343] (mis=1)
382-432 ==> 0-50 on |540|TRBJ1-5|J-REGION| [len=50] (mis=3)
432-484 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 8 umis using 568 reads
cdr3 = CASSLLGDNQPQHF at 362, score = 9 + 8
umis assigned: [109, 159, 195, 215, 217, 405, 456, 516, 564]
of which 8 are surviving nonsolos
reads assigned: 8564
start codons at 35, 317, 321, 408
confident = true

TIG 2[bases=620]
175-511 ==> 0-336 on |512|TRAV41|L-REGION+V-REGION| [len=336] (mis=3)
519-578 ==> 0-59 on |421|TRAJ35|J-REGION| [len=59] (mis=1)
578-620 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 5 umis using 112 reads
cdr3 = CAVFVPPIGFGNVLHC at 499, score = 6 + 6
umis assigned: [31, 164, 200, 280, 420, 429, 553]
of which 7 are surviving nonsolos
reads assigned: 2844
start codons at 14, 46, 80, 175, 245, 382, 407, 533
confident = true
now this is a cell
paired!

AGAGACTCTGCCGTCTACATCTGTGCTGTCTTCGTCCCGCCCATAGGCTTTGGGAATGTGCTGCATTGCGGGTCCGGCACTCAAGTGATTGTTTTACCAC <==> TTGGAGCTGGGGGACTCGGCCCTTTATCTTTGCGCCAGCAGCTTGTTGGGGGACAATCAGCCCCAGCATTTTGGTGATGGGACTCGACTCTCCATCCTAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.887 = CGTGAGCCAATAGAGT-1

using 76 reads

====================================================================================

graph has 76 edges initially, 4 edges after simplification

total ucounts = 13
nonsolo ucounts = 8[2^2, 4, 6^2, 8, 17, 26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.890 = CGTGAGCCACACATGT-1

using 21 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.891 = CGTGAGCCACAGTCGC-1

using 81 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[81]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.893 = CGTGAGCCACGCTTTC-1

using 35 reads

====================================================================================

graph has 48 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.895 = CGTGAGCCACTTAACG-1

using 7899 reads

====================================================================================

graph has 3289 edges initially, 58 edges after simplification

total ucounts = 285
nonsolo ucounts = 175[2^40, 3^32, 4^18, 5^10, 6^10, 7^6, 8^6, 9^5, 10^2, 11^3, 12^4, 13^2, 14, 15^3, 16, 17^4, 18^2, 19^2, 20, 21, 22, 23, 24, 25^3, 26, 28, 32, 33, 57, 69, 82, 86, 119, 126, 359, 422, 628, 1001, 1803, 1896]
surviving nonsolo ucounts = 8[69, 126, 359, 422, 628, 1001, 1803, 1896]
ids = [64, 69, 275, 62, 238, 113, 65, 226]

====================================================================================

UMI info for barcode CGTGAGCCACTTAACG-1 contig 1 = AGCTCTGCAG...
umi ATTTGGTCAA = 434 reads: +117 -1XX +253 -19 +7 invalidated
umi CGGCCTGTAG = 997 reads: +397 validated
umi TGACATACGC = 636 reads: +397 validated
umi TTTAGTCATG = 389 reads: -21 +1 -16XX +2 -2XX +1 -1XX +1 -1XX +351 invalidated

GOOD CONTIGS

TIG 1[bases=647]
0-198 ==> 3-201 on |634|TRBV5-4|5'UTR| [len=201] (mis=1)
198-541 ==> 0-343 on |635|TRBV5-4|L-REGION+V-REGION| [len=343] (mis=4)
551-595 ==> 7-51 on |544|TRBJ2-2|J-REGION| [len=51] (mis=0)
595-647 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 3 umis using 88 reads
cdr3 = CASSLREGHGELFF at 525, score = 8 + 9
umis assigned: [62, 113, 238, 275]
of which 4 are surviving nonsolos
reads assigned: 2376
start codons at 64, 198, 320, 418, 484
confident = true

REJECT CONTIGS

TIG 1[bases=444]
0-52 ==> 7-59 on |567|TRBV12-4|5'UTR| [len=59] (mis=0)
52-387 ==> 0-335 on |568|TRBV12-4|L-REGION+V-REGION| [len=347] (mis=0)
392-444 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
umis assigned: [64, 65, 69, 226]
of which 4 are surviving nonsolos
reads assigned: 3846
start codons at 0, 52, 110, 151, 174, 223, 226, 275, 286, 313, 320
confident = false
did not find CDR3
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.897 = CGTGAGCCAGATCCAT-1

using 19 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 1[17]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.900 = CGTGAGCCAGGGATTG-1

using 61 reads

====================================================================================

graph has 58 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[22, 39]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.901 = CGTGAGCCAGTCAGCC-1

using 31 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.903 = CGTGAGCCATCGATTG-1

using 23 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.908 = CGTGAGCCATTGGGCC-1

using 14 reads

====================================================================================

graph has 4 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[14]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.909 = CGTGAGCCATTTCACT-1

using 99 reads

====================================================================================

graph has 98 edges initially, 10 edges after simplification

total ucounts = 11
nonsolo ucounts = 8[2, 3^2, 9, 12, 14^2, 39]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.915 = CGTGAGCGTAGGGTAC-1

using 51 reads

====================================================================================

graph has 82 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 3[5, 9, 34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.921 = CGTGAGCGTCTCTCTG-1

using 375 reads

====================================================================================

graph has 456 edges initially, 6 edges after simplification

total ucounts = 101
nonsolo ucounts = 32[2^10, 3^2, 4, 5, 6^3, 7^2, 8, 9, 12, 13, 15, 17, 18, 19^2, 22, 26, 30, 31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.922 = CGTGAGCGTCTTGCGG-1

using 68 reads

====================================================================================

graph has 78 edges initially, 2 edges after simplification

total ucounts = 22
nonsolo ucounts = 13[2^4, 4^2, 5^2, 6^3, 7, 8]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.931 = CGTGAGCGTTCTCATT-1

using 341 reads

====================================================================================

graph has 318 edges initially, 8 edges after simplification

total ucounts = 33
nonsolo ucounts = 28[2, 3, 4^2, 5^2, 6^9, 8^3, 9, 11, 12^2, 19, 28, 31, 35, 36, 42]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.934 = CGTGAGCTCAACACTG-1

using 53 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[53]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.938 = CGTGAGCTCACCCTCA-1

using 298 reads

====================================================================================

graph has 318 edges initially, 10 edges after simplification

total ucounts = 25
nonsolo ucounts = 22[2^2, 3^2, 5^3, 6, 7^2, 8, 9, 10, 12, 13, 14, 16, 17, 31, 32, 34, 54]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.939 = CGTGAGCTCACGAAGG-1

using 64 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[64]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.950 = CGTGAGCTCCTGCTTG-1

using 94 reads

====================================================================================

graph has 52 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[8, 85]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.951 = CGTGAGCTCGACCAGC-1

using 40 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[40]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.953 = CGTGAGCTCGCCTGAG-1

using 42 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[4, 7, 29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.960 = CGTGAGCTCGGCGCTA-1

using 18358 reads

====================================================================================

graph has 5282 edges initially, 58 edges after simplification

total ucounts = 102
nonsolo ucounts = 58[2^14, 3^7, 4^6, 5^2, 6^2, 7^2, 8^2, 12, 19, 22, 23, 27, 29, 37, 51, 159, 499, 503, 712, 801, 870, 891, 976, 1125, 1147, 1368, 1386, 1519, 2517, 3496]
surviving nonsolo ucounts = 15[159, 499, 503, 712, 801, 870, 891, 976, 1125, 1147, 1368, 1386, 1519, 2517, 3496]
ids = [9, 57, 3, 81, 5, 50, 47, 20, 100, 70, ...]

====================================================================================

UMI info for barcode CGTGAGCTCGGCGCTA-1 contig 1 = ATTATTTTTT...
umi ATTATCATAC = 973 reads: +403 validated
umi CGCACTCCTT = 876 reads: +403 validated
umi CGTGCACTGT = 849 reads: +403 validated
umi TATACGGGCT = 706 reads: +403 validated

UMI info for barcode CGTGAGCTCGGCGCTA-1 contig 2 = AGAGGCCCCA...
umi ACAGGTCCGG = 827 reads: +400 validated
umi CCGGCAAGGT = 1364 reads: +398 -1XX +1 invalidated
umi TTTCCCACGC = 1134 reads: +400 validated

GOOD CONTIGS

TIG 1[bases=501]
0-56 ==> 0-56 on |461|TRAV12-3|5'UTR| [len=56] (mis=0)
56-399 ==> 0-343 on |462|TRAV12-3|L-REGION+V-REGION| [len=343] (mis=3)
396-459 ==> 0-63 on |435|TRAJ48|J-REGION| [len=63] (mis=4)
459-501 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 4 umis using 140 reads
cdr3 = CAMGTYFGNEKLTF at 386, score = 9 + 10
umis assigned: [20, 47, 50, 81]
of which 4 are surviving nonsolos
reads assigned: 3346
start codons at 56, 59, 221, 263, 294, 392, 411
confident = true

TIG 2[bases=481]
0-29 ==> 82-111 on |628|TRBV4-3|5'UTR| [len=111] (mis=0)
29-373 ==> 0-344 on |629|TRBV4-3|L-REGION+V-REGION| [len=344] (mis=3)
379-429 ==> 0-50 on |543|TRBJ2-1|J-REGION| [len=50] (mis=3)
429-481 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 2 umis using 149 reads
cdr3 = CASSPGTSFNNEQFF at 356, score = 10 + 8
umis assigned: [5, 41, 100]
of which 3 are surviving nonsolos
reads assigned: 3243
start codons at 29, 83, 122, 128, 151, 179, 221, 286, 387
confident = true

REJECT CONTIGS

TIG 1[bases=587]
0-36 ==> 0-36 on |455|TRAV10|5'UTR| [len=36] (mis=0)
36-379 ==> 0-343 on |456|TRAV10|L-REGION+V-REGION| [len=343] (mis=2)
486-545 ==> 0-59 on |412|TRAJ27|J-REGION| [len=59] (mis=0)
545-587 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
cdr3 = CVVSSYSGAGSYQLTF at 366, score = 6 + 10
umis assigned: [3, 9, 31]
of which 3 are surviving nonsolos
reads assigned: 3117
start codons at 36, 94, 164, 246, 298, 494, 518
confident = false
frameshifted full length stopped transcript of length 587
VJ delta = -79
delta too large
not full
not full

TIG 2[bases=494]
0-47 ==> 0-47 on |692|TRBV9|5'UTR| [len=47] (mis=0)
47-390 ==> 0-343 on |693|TRBV9|L-REGION+V-REGION| [len=343] (mis=0)
392-442 ==> 0-50 on |543|TRBJ2-1|J-REGION| [len=50] (mis=4)
442-494 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
umis assigned: [46, 57, 59, 67, 70]
of which 5 are surviving nonsolos
reads assigned: 7677
start codons at 3, 47, 169, 255, 400
confident = false
did not find CDR3
now this is a cell
paired!

CAGCCCAGTGATTCAGCCACCTACCTCTGTGCAATGGGGACTTACTTTGGAAATGAGAAATTAACCTTTGGGACTGGAACAAGACTCACCATCATACCCA <==> CAGCCAGAAGACTCGGCCCTGTATCTCTGCGCCAGCAGCCCCGGGACTAGCTTTAACAATGAGCAGTTCTTCGGGCCAGGGACACGGCTCACCGTGCTAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.961 = CGTGAGCTCGGTGTTA-1

using 33 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[12, 20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.963 = CGTGAGCTCTAACTCT-1

using 1033 reads

====================================================================================

graph has 318 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[2, 1029]
surviving nonsolo ucounts = 1[1029]
ids = [2]

====================================================================================

UMI info for barcode CGTGAGCTCTAACTCT-1 contig 1 = AGCTCCTGTA...
umi TGAGGGTTCA = 1036 reads: +400 validated

GOOD CONTIGS

TIG 1[bases=516]
0-64 ==> 0-64 on |571|TRBV12-5|5'UTR| [len=64] (mis=0)
64-411 ==> 0-347 on |572|TRBV12-5|L-REGION+V-REGION| [len=347] (mis=0)
424-464 ==> 10-50 on |543|TRBJ2-1|J-REGION| [len=50] (mis=0)
464-516 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 1 umis using 61 reads
cdr3 = CASGLVQLAGEQFF at 394, score = 8 + 8
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 1021
start codons at 64, 122, 163, 181, 186, 235, 238, 287, 298, 325, 332
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.972 = CGTGAGCTCTTAACCT-1

using 25 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.980 = CGTGTAAAGCACCGTC-1

using 12781 reads

====================================================================================

graph has 11640 edges initially, 248 edges after simplification

total ucounts = 2457
nonsolo ucounts = 1862[2^363, 3^280, 4^213, 5^161, 6^138, 7^112, 8^93, 9^81, 10^74, 11^64, 12^53, 13^40, 14^39, 15^29, 16^32, 17^22, 18^14, 19^17, 20^10, 21^6, 22^10, 23^2, 24, 25^3, 26, 27, 29, 31, 32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.982 = CGTGTAAAGGATGGAA-1

using 42 reads

====================================================================================

graph has 54 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 6[2^2, 4, 8, 11, 14]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.985 = CGTGTAAAGTAAGTAC-1

using 181 reads

====================================================================================

graph has 66 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[180]
surviving nonsolo ucounts = 1[180]
ids = [1]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.988 = CGTGTAAAGTCTCAAC-1

using 2119 reads

====================================================================================

graph has 1117 edges initially, 14 edges after simplification

total ucounts = 115
nonsolo ucounts = 54[2^20, 3^7, 4, 5, 6^3, 7^3, 8, 10, 11, 16, 17, 19, 22, 41, 43, 52, 65, 86, 130, 151, 154, 231, 246, 282, 365]
surviving nonsolo ucounts = 12[41, 43, 52, 65, 86, 130, 151, 154, 231, 246, 282, 365]
ids = [88, 17, 55, 28, 34, 24, 73, 61, 98, 31, ...]

====================================================================================

UMI info for barcode CGTGTAAAGTCTCAAC-1 contig 1 = TTCCTCTGCT...
umi CATCATGTAG = 128 reads: +391 validated
umi CCGTCTTGTT = 244 reads: +391 validated
umi GCACCGGATC = 42 reads: -362X +1 -4X +1 -8XX +1 -12XX +2 invalidated
umi GCTTCACCGA = 14 reads: -385X +1 -1X +4 invalidated
umi GTACGCGGCA = 152 reads: +345 -1 +7 -2 +1 -1 +34 non-validated
umi GTTTTGCCAC = 153 reads: +383 -1 +7 non-validated
umi TGTTGCTAGA = 233 reads: +391 validated

GOOD CONTIGS

TIG 1[bases=555]
0-112 ==> 0-112 on |618|TRBV30|5'UTR| [len=112] (mis=0)
112-447 ==> 0-335 on |619|TRBV30|L-REGION+V-REGION| [len=335] (mis=0)
453-503 ==> 0-50 on |540|TRBJ1-5|J-REGION| [len=50] (mis=4)
503-555 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 5 umis using 47 reads
cdr3 = CAWSVGAANQPQHF at 433, score = 7 + 8
umis assigned: [24, 31, 55, 57, 61, 73, 98]
of which 6 are surviving nonsolos
reads assigned: 947
start codons at 109, 112, 180, 479
confident = true

REJECT CONTIGS

TIG 1[bases=633]
0-238 ==> 5098-5336 on segment before TRAV8-3 exon 1 [len=5336] (mis=0)
238-579 ==> 0-341 on |522|TRAV8-3|L-REGION+V-REGION| [len=341] (mis=3)
578-633 ==> 0-55 on |405|TRAJ20|J-REGION| [len=57] (mis=5)
cdr3 = CAVAPRNDYKLSF at 565, score = 7 + 8
umis assigned: [17, 28, 34, 88]
of which 4 are surviving nonsolos
reads assigned: 214
start codons at 238, 360, 374, 401, 548
confident = false
VJ delta = 20
not full
not full

TIG 2[bases=463]
16-354 ==> 0-338 on |477|TRAV2|L-REGION+V-REGION| [len=338] (mis=2)
365-421 ==> 7-63 on |425|TRAJ39|J-REGION| [len=63] (mis=0)
421-463 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
cdr3 = CCLLLCCGAPGGVNAGNMLTF at 327, score = 4 + 9
umis assigned: [47, 56]
of which 2 are surviving nonsolos
reads assigned: 641
start codons at 16, 173, 210, 268, 275, 323, 367, 378, 408
confident = false
frameshifted full length stopped transcript of length 463
VJ delta = 39
delta too large
not full
not full
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1000 = CGTGTAACAAGCGTAG-1

using 538 reads

====================================================================================

graph has 502 edges initially, 2 edges after simplification

total ucounts = 99
nonsolo ucounts = 48[2^16, 3^6, 4^5, 5^2, 6^6, 7^5, 8, 9^2, 10^2, 11^2, 267]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================

REJECT CONTIGS

TIG 1[bases=307]
5-78 ==> 727-800 on segment before TRAJ12 exon 1 [len=800] (mis=0)
201-265 ==> 0-64 on |395|TRAJ10|J-REGION| [len=64] (mis=0)
265-307 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
umis assigned: [2]
of which 0 are surviving nonsolos
reads assigned: 262
start codons at 24, 33, 80
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1047 = CGTGTAATCGATAGAA-1

using 3091 reads

====================================================================================

graph has 1218 edges initially, 34 edges after simplification

total ucounts = 100
nonsolo ucounts = 35[2^10, 3^4, 4^2, 5^3, 6, 9, 11, 13, 14^2, 18, 95, 97, 108, 220, 226, 253, 447, 568, 872]
surviving nonsolo ucounts = 9[95, 97, 108, 220, 226, 253, 447, 568, 872]
ids = [2, 44, 65, 83, 14, 6, 29, 33, 38]

====================================================================================

UMI info for barcode CGTGTAATCGATAGAA-1 contig 1 = GACTGATCTG...
umi CATACTGTAC = 252 reads: +403 validated
umi CTCCAGTCTC = 708 reads: -371X +6 -2XX +1 -1XX +2 -1XX +5 -1XX +5 -1XX +2 -3XX +2 invalidated
umi CTGTGACCGT = 96 reads: +403 validated

UMI info for barcode CGTGTAATCGATAGAA-1 contig 2 = CTGGAAGACC...
umi ATTCATATGT = 79 reads: +342 -85 non-validated
umi CCATAGACCA = 170 reads: +427 validated
umi GTTGTAGGGC = 92 reads: +379 -48 non-validated

GOOD CONTIGS

TIG 1[bases=495]
40-387 ==> 0-347 on |687|TRBV7-8|L-REGION+V-REGION| [len=347] (mis=2)
384-396 ==> 0-12 on |535|TRBD1|D-REGION| [len=12] (mis=3)
395-443 ==> 0-48 on |536|TRBJ1-1|J-REGION| [len=48] (mis=2)
443-495 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 2 umis using 44 reads
cdr3 = CASSLRQGVSTEAFF at 370, score = 8 + 8
umis assigned: [6, 38, 44]
of which 3 are surviving nonsolos
reads assigned: 1040
start codons at 40, 149, 182, 248
confident = true

TIG 2[bases=472]
0-45 ==> 0-45 on |484|TRAV23/DV6|5'UTR| [len=45] (mis=0)
45-409 ==> 0-364 on |485|TRAV23/DV6|L-REGION+V-REGION| [len=364] (mis=3)
406-472 ==> 0-66 on |429|TRAJ42|J-REGION| [len=66] (mis=0)
junction support: 1 umis using 11 reads
cdr3 = CAAMNYGGSQGNLIF at 396, score = 9 + 10
umis assigned: [2, 14, 65]
of which 3 are surviving nonsolos
reads assigned: 330
start codons at 45, 92, 202, 230, 286, 360, 405, 412
confident = true

REJECT CONTIGS

TIG 1[bases=407]
4-50 ==> 0-46 on |545|TRBJ2-2P|J-REGION| [len=46] (mis=0)
50-154 ==> 0-104 on segment before TRBJ2-3 exon 1 [len=104] (mis=0)
203-305 ==> 0-102 on segment before TRBJ2-4 exon 1 [len=102] (mis=0)
355-407 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
umis assigned: [29, 33, 83]
of which 3 are surviving nonsolos
reads assigned: 1201
start codons at 64
confident = false
did not find CDR3
now this is a cell
paired!

CCTGGAGACTCAGCCACCTACTTCTGTGCAGCAATGAATTATGGAGGAAGCCAAGGAAATCTCATCTTTGGAAAAGGCACTAAACTCTCTGTTAAACCAA <==> CAGCAGGAGGACTCCGCCGTGTATCTCTGTGCCAGCAGCTTAAGACAGGGGGTAAGCACTGAAGCTTTCTTTGGACAAGGCACCAGACTCACAGTTGTAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1052 = CGTGTAATCGTTGCCT-1

using 1235 reads

====================================================================================

graph has 856 edges initially, 6 edges after simplification

total ucounts = 173
nonsolo ucounts = 62[2^22, 3^7, 4^4, 5^8, 6^3, 7^2, 8^2, 9^3, 10, 11, 12^2, 14^2, 15, 19^2, 30, 772]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================

REJECT CONTIGS

TIG 1[bases=306]
4-77 ==> 727-800 on segment before TRAJ12 exon 1 [len=800] (mis=0)
200-264 ==> 0-64 on |395|TRAJ10|J-REGION| [len=64] (mis=0)
264-306 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
umis assigned: [76]
of which 0 are surviving nonsolos
reads assigned: 762
start codons at 23, 32, 79
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1059 = CGTGTCTAGAAACCGC-1

using 28 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1069 = CGTGTCTAGCCTATGT-1

using 589 reads

====================================================================================

graph has 102 edges initially, 4 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[22, 567]
surviving nonsolo ucounts = 1[567]
ids = [1]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1071 = CGTGTCTAGCGATATA-1

using 9 reads

====================================================================================

graph has 4 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[9]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1076 = CGTGTCTAGGAGCGTT-1

using 27 reads

====================================================================================

graph has 6 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1080 = CGTGTCTAGGCCCGTT-1

using 120 reads

====================================================================================

graph has 140 edges initially, 10 edges after simplification

total ucounts = 18
nonsolo ucounts = 12[2^2, 3, 4, 5, 7^2, 9, 13, 14, 16, 32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1084 = CGTGTCTAGGTGGGTT-1

using 201 reads

====================================================================================

graph has 248 edges initially, 4 edges after simplification

total ucounts = 28
nonsolo ucounts = 22[2, 3^3, 4^2, 5^3, 6, 8, 9, 10^4, 11^2, 13, 15, 18, 30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1093 = CGTGTCTAGTGTACGG-1

using 27 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1102 = CGTGTCTCAACTGCTA-1

using 3299 reads

====================================================================================

graph has 1412 edges initially, 18 edges after simplification

total ucounts = 223
nonsolo ucounts = 102[2^42, 3^15, 4^8, 5^5, 6^4, 7^3, 8^3, 9^4, 10^3, 11^2, 12, 13, 14^4, 15, 18, 20, 21, 33, 40, 2607]
surviving nonsolo ucounts = 2[40, 2607]
ids = [65, 79]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1103 = CGTGTCTCAAGAAGAG-1

using 112 reads

====================================================================================

graph has 142 edges initially, 2 edges after simplification

total ucounts = 16
nonsolo ucounts = 12[2^6, 7, 11, 14^2, 20, 30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1105 = CGTGTCTCAAGAGGCT-1

using 40 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 2[2, 33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1108 = CGTGTCTCAAGTAGTA-1

using 19 reads

====================================================================================

graph has 6 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1111 = CGTGTCTCAATGGATA-1

using 42 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[42]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1114 = CGTGTCTCACCAGTTA-1

using 211 reads

====================================================================================

graph has 68 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[2^2, 206]
surviving nonsolo ucounts = 1[206]
ids = [2]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1117 = CGTGTCTCACGCATCG-1

using 1682 reads

====================================================================================

graph has 1137 edges initially, 24 edges after simplification

total ucounts = 227
nonsolo ucounts = 109[2^43, 3^22, 4^11, 5^4, 6^9, 7^2, 8, 10, 11^5, 12, 13, 14^2, 20, 21^2, 30, 41, 83, 938]
surviving nonsolo ucounts = 1[938]
ids = [160]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1127 = CGTGTCTCAGTATGCT-1

using 3834 reads

====================================================================================

graph has 2090 edges initially, 40 edges after simplification

total ucounts = 353
nonsolo ucounts = 189[2^86, 3^35, 4^24, 5^7, 6^3, 7^2, 8, 9^5, 10, 11^2, 12^2, 13^2, 14^2, 15, 18^3, 19, 21, 22, 25, 29, 36, 59, 77, 174, 175, 193, 392, 1776]
surviving nonsolo ucounts = 8[11, 25, 59, 77, 175, 193, 392, 1776]
ids = [102, 268, 7, 133, 227, 274, 195, 35]

====================================================================================

UMI info for barcode CGTGTCTCAGTATGCT-1 contig 1 = GCTTCACACA...
umi TCCCTCTCTG = 192 reads: +397 validated

GOOD CONTIGS

TIG 1[bases=608]
211-554 ==> 0-343 on |643|TRBV5-6|L-REGION+V-REGION| [len=343] (mis=1)
557-608 ==> 0-51 on |539|TRBJ1-4|J-REGION| [len=51] (mis=0)
junction support: 1 umis using 4 reads
cdr3 = CASSLHATNEKLFF at 538, score = 8 + 8
umis assigned: [274]
of which 1 are surviving nonsolos
reads assigned: 188
start codons at 77, 184, 211, 333, 353, 416, 497, 554, 563
confident = true

REJECT CONTIGS

TIG 1[bases=311]
5-200 ==> 5805-6000 on segment before TRBD1 exon 1 [len=6000] (mis=0)
211-259 ==> 0-48 on |537|TRBJ1-2|J-REGION| [len=48] (mis=2)
259-311 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
umis assigned: [35, 133, 195, 227, 268]
of which 5 are surviving nonsolos
reads assigned: 1779
start codons at 77, 87, 161, 217
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1140 = CGTGTCTGTATTCGTG-1

using 39 reads

====================================================================================

graph has 52 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[39]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1156 = CGTGTCTTCACTATTC-1

using 16139 reads

====================================================================================

graph has 6874 edges initially, 84 edges after simplification

total ucounts = 1004
nonsolo ucounts = 586[2^197, 3^108, 4^82, 5^44, 6^31, 7^26, 8^13, 9^10, 10^8, 11^3, 12^6, 13^4, 14^2, 15, 16^4, 17^5, 18^2, 19^4, 21, 22^2, 23^2, 25, 28, 29, 30, 31^2, 36, 42, 45, 52, 64, 70, 122, 153, 172, 296, 329, 356, 398, 413, 440, 454, 490, 585, 694, 721, 809, 962, 1488, 1924, 1951]
surviving nonsolo ucounts = 20[70, 122, 153, 172, 296, 329, 356, 398, 413, 440, 454, 490, 585, 694, 721, 809, 962, 1488, 1924, 1951]
ids = [852, 509, 321, 121, 265, 47, 618, 78, 492, 51, ...]

====================================================================================

UMI info for barcode CGTGTCTTCACTATTC-1 contig 1 = AGTGACACTG...
umi AATCTAGCTT = 397 reads: +390 -2XX +1 -4XX invalidated
umi ACTCTCTCCT = 173 reads: +372 -1 +24 non-validated
umi CAATGTAGGC = 292 reads: +397 validated
umi CCTCTCTGGT = 696 reads: +397 validated
umi CGCGACCTGT = 1585 reads: -378X +3 -1XX +2 -1XX +1 -1XX +2 -1XX +2 -2XX +1 -1XX +1 invalidated
umi CTAACAACGG = 1986 reads: +83 -2XX +1 -10XX +1 -8XX +2 -3XX +2 -2X +1 -1X +2 -115X +1 -1XX +3 -3XX +1 -3XX +2 -9XX +141 invalidated
umi GTAACCATAC = 823 reads: +397 validated
umi GTCTTGGTCT = 590 reads: +397 validated
umi TCTAGGCCGG = 713 reads: -11X +1 -1XX +1 -2XX +1 -8XX +2 -1XX +1 -1XX +1 -4XX +2 -3XX +1 -4XX +352 invalidated
umi TGAGTCGCTG = 1495 reads: -40X +1 -4XX +352 invalidated
umi TTGCCCGCTA = 960 reads: -34 +1 -5XX +1 -4XX +352 invalidated

UMI info for barcode CGTGTCTTCACTATTC-1 contig 2 = AAAGCAGATT...
umi AATTACCCAT = 443 reads: +400 validated
umi ACAAATTTAG = 493 reads: +400 validated
umi ACCACATCGC = 396 reads: +400 validated
umi CATTTCTCGT = 152 reads: +362 -19 +19 non-validated
umi CTGCCGTTCC = 412 reads: +400 validated

GOOD CONTIGS

TIG 1[bases=494]
0-45 ==> 0-45 on |682|TRBV7-6|5'UTR| [len=45] (mis=1)
45-392 ==> 0-347 on |683|TRBV7-6|L-REGION+V-REGION| [len=347] (mis=5)
394-442 ==> 0-48 on |537|TRBJ1-2|J-REGION| [len=48] (mis=3)
442-494 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 10 umis using 636 reads
cdr3 = CASSGNPGYGYTF at 375, score = 5 + 9
umis assigned: [47, 121, 265, 371, 399, 444, 661, 694, 837, 870] and 1 others
of which 11 are surviving nonsolos
reads assigned: 9449
start codons at 45, 62, 154, 187, 253, 286, 366, 400
confident = true

TIG 2[bases=547]
0-105 ==> 0-105 on |459|TRAV12-2|5'UTR| [len=105] (mis=0)
105-445 ==> 0-340 on |460|TRAV12-2|L-REGION+V-REGION| [len=340] (mis=2)
439-505 ==> 0-66 on |418|TRAJ32|J-REGION| [len=66] (mis=3)
505-547 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 4 umis using 80 reads
cdr3 = CAVKAGGATNKLIF at 432, score = 9 + 8
umis assigned: [51, 60, 78, 321, 492]
of which 5 are surviving nonsolos
reads assigned: 1869
start codons at 16, 102, 105, 309, 325, 340, 379
confident = true

REJECT CONTIGS

TIG 1[bases=515]
0-69 ==> 0-69 on |478|TRAV20|5'UTR| [len=69] (mis=0)
69-406 ==> 0-337 on |479|TRAV20|L-REGION+V-REGION| [len=337] (mis=0)
409-473 ==> 0-64 on |395|TRAJ10|J-REGION| [len=64] (mis=0)
473-515 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
umis assigned: [13, 509, 618, 852]
of which 4 are surviving nonsolos
reads assigned: 968
start codons at 69, 78
confident = false
frameshifted full length transcript of length 515
did not find CDR3
now this is a cell
paired!

CAGCCCAGTGATTCAGCCACCTACCTCTGTGCCGTGAAAGCTGGTGGTGCTACAAACAAGCTCATCTTTGGAACTGGCACTCTGCTTGCTGTCCAGCCAA <==> CGCACAGAGCAGCGGGACTCGGCCATGTATCGCTGTGCCAGCAGCGGTAATCCAGGCTATGGCTACACCTTCGGTTCGGGGACCAGGTTAACCGTTGTAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1165 = CGTGTCTTCCAGAAGG-1

using 64 reads

====================================================================================

graph has 72 edges initially, 2 edges after simplification

total ucounts = 12
nonsolo ucounts = 7[2, 3, 4, 5, 12, 16, 17]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1167 = CGTGTCTTCCGAATGT-1

using 31 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 1[29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1179 = CGTGTCTTCTTACCTA-1

using 26 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 4[2^2, 3, 18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1184 = CGTTAGAAGAAGAAGC-1

using 90 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[90]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1185 = CGTTAGAAGAAGATTC-1

using 63 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[63]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1190 = CGTTAGAAGACGCTTT-1

using 28 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1192 = CGTTAGAAGAGTCGGT-1

using 35 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 3[7, 12, 16]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1198 = CGTTAGAAGCAGATCG-1

using 37 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1207 = CGTTAGAAGGAGTACC-1

using 40 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[40]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1208 = CGTTAGAAGGCTAGGT-1

using 1160 reads

====================================================================================

graph has 870 edges initially, 26 edges after simplification

total ucounts = 149
nonsolo ucounts = 94[2^19, 3^12, 4^9, 5^8, 6^9, 7^2, 8, 9^3, 10^3, 11^2, 12^2, 13, 14^5, 17, 19, 20^2, 22^3, 23, 25, 27, 30, 37, 39, 44, 56, 61, 104, 105]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1209 = CGTTAGAAGGGTGTGT-1

using 51 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[51]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1212 = CGTTAGAAGTAAGTAC-1

using 69 reads

====================================================================================

graph has 70 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 4[12, 15^2, 24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1216 = CGTTAGAAGTCTCCTC-1

using 40 reads

====================================================================================

graph has 56 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[14, 26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1217 = CGTTAGAAGTCTTGCA-1

using 3380 reads

====================================================================================

graph has 2844 edges initially, 82 edges after simplification

total ucounts = 333
nonsolo ucounts = 266[2^30, 3^26, 4^17, 5^23, 6^14, 7^14, 8^11, 9^10, 10^11, 11^12, 12^5, 13^12, 14^5, 15^5, 16^4, 17^15, 18^8, 19^2, 20^5, 21^3, 22^3, 23^3, 24, 25, 26, 27^2, 28, 29, 31^2, 32, 33^3, 37^3, 38, 39^2, 40, 42, 43, 47, 54^2, 107, 109, 128]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1220 = CGTTAGAAGTGTTTGC-1

using 1070 reads

====================================================================================

graph has 398 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 6[3, 5^2, 6, 26, 1022]
surviving nonsolo ucounts = 2[26, 1022]
ids = [2, 3]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1226 = CGTTAGACAAGGTTTC-1

using 212 reads

====================================================================================

graph has 154 edges initially, 6 edges after simplification

total ucounts = 10
nonsolo ucounts = 8[2, 3, 14, 21, 30, 35, 42, 63]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1229 = CGTTAGACAATAGCGG-1

using 1174 reads

====================================================================================

graph has 912 edges initially, 32 edges after simplification

total ucounts = 187
nonsolo ucounts = 134[2^29, 3^19, 4^18, 5^13, 6^9, 7^9, 8^5, 9^3, 10, 11^2, 12^3, 13^4, 14^2, 15^4, 16, 18^2, 23, 26^2, 29, 31, 36, 49^2, 78^2]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1233 = CGTTAGACACCAGTTA-1

using 1356 reads

====================================================================================

graph has 448 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 2[5, 1348]
surviving nonsolo ucounts = 1[1348]
ids = [0]

====================================================================================

UMI info for barcode CGTTAGACACCAGTTA-1 contig 1 = GGGACAGTGA...
umi ACGGCTTAGT = 1356 reads: +397 validated

GOOD CONTIGS

TIG 1[bases=499]
0-50 ==> 0-50 on |678|TRBV7-3|5'UTR| [len=50] (mis=3)
50-397 ==> 0-347 on |679|TRBV7-3|L-REGION+V-REGION| [len=347] (mis=5)
406-447 ==> 9-50 on |543|TRBJ2-1|J-REGION| [len=50] (mis=0)
447-499 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 1 umis using 100 reads
cdr3 = CASSSQSGVEQFF at 380, score = 8 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 1338
start codons at 50, 159, 273
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1235 = CGTTAGACACTCGACG-1

using 37 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1239 = CGTTAGACAGGATCGA-1

using 35 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[4, 9, 20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1242 = CGTTAGACAGGTGGAT-1

using 44 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[7, 36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1252 = CGTTAGACATCTGGTA-1

using 75 reads

====================================================================================

graph has 100 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 9[2, 4^2, 5, 7, 10, 12^2, 18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1254 = CGTTAGACATGAGCGA-1

using 2168 reads

====================================================================================

graph has 694 edges initially, 4 edges after simplification

total ucounts = 7
nonsolo ucounts = 3[8, 29, 2127]
surviving nonsolo ucounts = 1[2127]
ids = [3]

====================================================================================

UMI info for barcode CGTTAGACATGAGCGA-1 contig 1 = AAAGCTCCCA...
umi CGGGTATTTC = 2131 reads: +403 validated

GOOD CONTIGS

TIG 1[bases=483]
0-28 ==> 11-39 on |672|TRBV7-2|5'UTR| [len=39] (mis=0)
28-375 ==> 0-347 on |673|TRBV7-2|L-REGION+V-REGION| [len=347] (mis=1)
380-431 ==> 0-51 on |544|TRBJ2-2|J-REGION| [len=51] (mis=5)
431-483 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 1 umis using 152 reads
cdr3 = CASSLTGVGTGELFF at 358, score = 8 + 9
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 2096
start codons at 28, 137
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1259 = CGTTAGAGTAAACCTC-1

using 46 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[46]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1261 = CGTTAGAGTACCGGCT-1

using 1104 reads

====================================================================================

graph has 713 edges initially, 26 edges after simplification

total ucounts = 61
nonsolo ucounts = 43[2^4, 3^2, 4^2, 5^4, 6^2, 7^2, 8, 9^2, 10^2, 11, 15, 17^2, 19, 20, 22, 24^3, 25, 26, 27, 31, 35, 45, 66, 70, 80, 109, 116, 149]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1268 = CGTTAGAGTCAGGACA-1

using 122 reads

====================================================================================

graph has 52 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[122]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1270 = CGTTAGAGTCATACTG-1

using 32 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 5[3, 4^2, 7, 11]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1280 = CGTTAGAGTGGCTCCA-1

using 39 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 3[6, 14, 19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1281 = CGTTAGAGTTACGACT-1

using 47 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 6[2, 4^2, 5, 14, 15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1282 = CGTTAGAGTTATTCTC-1

using 40 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[40]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1283 = CGTTAGAGTTCAGCGC-1

using 53 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[52]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1284 = CGTTAGAGTTCGTCTC-1

using 337 reads

====================================================================================

graph has 223 edges initially, 8 edges after simplification

total ucounts = 13
nonsolo ucounts = 11[2, 3, 6, 15, 16, 23, 26, 30^2, 86, 98]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1289 = CGTTAGAGTTTGACTG-1

using 451 reads

====================================================================================

graph has 214 edges initially, 10 edges after simplification

total ucounts = 13
nonsolo ucounts = 12[2^2, 4^3, 15, 22, 45, 54, 84, 85, 129]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1290 = CGTTAGATCAACACGT-1

using 2474 reads

====================================================================================

graph has 2440 edges initially, 82 edges after simplification

total ucounts = 279
nonsolo ucounts = 201[2^40, 3^19, 4^16, 5^16, 6^10, 7^12, 8^7, 9^7, 10^4, 11^4, 12^5, 13^2, 14^7, 16^3, 17^5, 18^5, 19^5, 20^4, 21^4, 23^2, 24, 25, 26^3, 28, 29, 30^2, 32, 33^2, 35, 38, 39, 41, 50, 52^2, 63, 66, 90^2, 98]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1292 = CGTTAGATCACATAGC-1

using 76 reads

====================================================================================

graph has 102 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 9[2^2, 3^2, 9, 12^2, 13, 19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1295 = CGTTAGATCAGGCGAA-1

using 22 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1298 = CGTTAGATCATAACCG-1

using 37 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[17, 19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1299 = CGTTAGATCATCACCC-1

using 77 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 1[74]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1301 = CGTTAGATCATGTCTT-1

using 57 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[57]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1302 = CGTTAGATCCACGAAT-1

using 54 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[54]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1305 = CGTTAGATCCCGGATG-1

using 3580 reads

====================================================================================

graph has 3333 edges initially, 86 edges after simplification

total ucounts = 481
nonsolo ucounts = 321[2^55, 3^30, 4^27, 5^28, 6^29, 7^13, 8^15, 9^14, 10^12, 11^10, 12^13, 13^7, 14^11, 15^9, 16^4, 18^2, 19^2, 20, 21, 22^2, 23^3, 24^2, 26^4, 27, 28^2, 29, 30, 31, 32, 33^2, 34^3, 35^2, 37, 39, 40, 41, 42, 48, 59, 63, 66, 71, 84, 97, 121]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1309 = CGTTAGATCCGTCAAA-1

using 58 reads

====================================================================================

graph has 60 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 5[3^2, 8, 10, 31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1310 = CGTTAGATCCGTCATC-1

using 99 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[99]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1315 = CGTTAGATCGCATGAT-1

using 37664 reads

====================================================================================

graph has 12815 edges initially, 146 edges after simplification

total ucounts = 620
nonsolo ucounts = 451[2^65, 3^39, 4^42, 5^19, 6^37, 7^32, 8^23, 9^13, 10^18, 11^18, 12^15, 13^12, 14^4, 15^16, 16^7, 17^13, 18^4, 19^3, 20^8, 21^5, 22^4, 23^4, 24^3, 25^2, 26^3, 27^3, 31^2, 34^3, 35, 36, 37^2, 38, 58, 62, 69, 71, 80, 108, 135, 191, 465, 575, 584, 660, 740, 819, 916, 1009, 1180, 1421, 1437, 1440, 1480, 1511, 1569, 1587, 1672, 1688, 1861, 2254, 8051]
surviving nonsolo ucounts = 23[62, 135, 465, 575, 584, 660, 740, 819, 916, 1009, 1180, 1421, 1437, 1440, 1480, 1511, 1569, 1587, 1672, 1688, 1861, 2254, 8051]
ids = [554, 35, 186, 108, 29, 603, 272, 17, 135, 544, ...]

====================================================================================

UMI info for barcode CGTTAGATCGCATGAT-1 contig 1 = AGGAGGACCA...
umi AAATTACGGT = 1578 reads: +403 validated
umi AAGCTACTCC = 192 reads: +200 -1 +43 -159 non-validated
umi AGATGATTGC = 1580 reads: +403 validated
umi CAAGTTCGGG = 1197 reads: +403 validated
umi CAGCACCGGG = 416 reads: -388X +2 -11XX +2 invalidated
umi CGTTCTTCCG = 702 reads: -388X +2 -11XX +2 invalidated
umi CTGTCACTTC = 1704 reads: +403 validated
umi CTTCTTGGCG = 1868 reads: +403 validated
umi GGAGCGACCG = 1451 reads: +403 validated
umi GTCAGCCTAT = 2257 reads: +403 validated
umi TCCATAGGAT = 1430 reads: +403 validated
umi TGGACGTTCG = 1008 reads: +403 validated
umi TGTCAGGGCC = 1690 reads: +403 validated

UMI info for barcode CGTTAGATCGCATGAT-1 contig 2 = GGGGATTCTG...
umi ACGTCGGCTA = 1511 reads: +397 validated
umi ATTAAGATAG = 1478 reads: +397 validated
umi TGTCATTTGC = 62 reads: +397 validated
umi TTGTACTCTA = 671 reads: +397 validated

GOOD CONTIGS

TIG 1[bases=506]
0-51 ==> 258-309 on |630|TRBV5-1|5'UTR| [len=309] (mis=0)
51-394 ==> 0-343 on |631|TRBV5-1|L-REGION+V-REGION| [len=343] (mis=0)
391-403 ==> 0-12 on |535|TRBD1|D-REGION| [len=12] (mis=3)
404-454 ==> 0-50 on |543|TRBJ2-1|J-REGION| [len=50] (mis=2)
454-506 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 10 umis using 808 reads
cdr3 = CASSLGGGLPYNEQFF at 378, score = 9 + 8
umis assigned: [6, 17, 89, 167, 186, 272, 313, 324, 387, 419] and 3 others
of which 13 are surviving nonsolos
reads assigned: 16743
start codons at 51, 333, 337, 412
confident = true

TIG 2[bases=515]
76-402 ==> 0-326 on |498|TRAV30|L-REGION+V-REGION| [len=337] (mis=1)
407-473 ==> 0-66 on |432|TRAJ45|J-REGION| [len=66] (mis=1)
473-515 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 4 umis using 230 reads
cdr3 = CRPYSGGGADGLTF at 400, score = 7 + 8
umis assigned: [67, 142, 554, 603]
of which 4 are surviving nonsolos
reads assigned: 3653
start codons at 76, 188, 251, 274, 308, 332
confident = true

REJECT CONTIGS

TIG 1[bases=312]
14-204 ==> 5800-5990 on segment before TRBD1 exon 1 [len=6000] (mis=0)
217-260 ==> 8-51 on |544|TRBJ2-2|J-REGION| [len=51] (mis=0)
260-312 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
umis assigned: [213, 258]
of which 2 are surviving nonsolos
reads assigned: 9347
start codons at 91, 101, 175
confident = false
did not find CDR3
now this is a cell
paired!

CAGCTCAGTTACTCAGGAACCTACTTCTGTCGGCCGTATTCAGGAGGAGGTGCTGACGGACTCACCTTTGGCAAAGGGACTCATCTAATCATCCAGCCCT <==> CTGGGGGACTCGGCCCTTTATCTTTGCGCCAGCAGCTTGGGCGGGGGGCTACCCTACAATGAGCAGTTCTTCGGGCCAGGGACACGGCTCACCGTGCTAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1322 = CGTTAGATCGTAGGAG-1

using 151 reads

====================================================================================

graph has 56 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 2[2, 146]
surviving nonsolo ucounts = 1[146]
ids = [4]

====================================================================================

UMI info for barcode CGTTAGATCGTAGGAG-1 contig 1 = GCACAGACAC...
umi CTTAATGTTT = 113 reads: +403 validated

GOOD CONTIGS

TIG 1[bases=444]
0-33 ==> 276-309 on |630|TRBV5-1|5'UTR| [len=309] (mis=0)
33-376 ==> 0-343 on |631|TRBV5-1|L-REGION+V-REGION| [len=343] (mis=0)
373-385 ==> 0-12 on |535|TRBD1|D-REGION| [len=12] (mis=3)
386-436 ==> 0-50 on |543|TRBJ2-1|J-REGION| [len=50] (mis=2)
junction support: 1 umis using 8 reads
cdr3 = CASSLGGGLPYNEQFF at 360, score = 9 + 8
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 112
start codons at 33, 315, 319, 394
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1325 = CGTTAGATCTCGCTTG-1

using 62 reads

====================================================================================

graph has 46 edges initially, 4 edges after simplification

total ucounts = 9
nonsolo ucounts = 7[3^2, 4^3, 5, 37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1335 = CGTTAGATCTTGCATT-1

using 27 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[2^2, 22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1336 = CGTTAGATCTTGCCGT-1

using 28 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1338 = CGTTCTGAGAACTCGG-1

using 187 reads

====================================================================================

graph has 146 edges initially, 6 edges after simplification

total ucounts = 19
nonsolo ucounts = 13[2^3, 3^3, 6, 7, 12, 28, 30, 37, 46]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1339 = CGTTCTGAGAAGCCCA-1

using 65 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[65]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1340 = CGTTCTGAGAATTGTG-1

using 116 reads

====================================================================================

graph has 118 edges initially, 2 edges after simplification

total ucounts = 19
nonsolo ucounts = 12[2^4, 3, 4^2, 11, 12, 13, 16, 38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1342 = CGTTCTGAGACTAAGT-1

using 47 reads

====================================================================================

graph has 56 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[47]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1344 = CGTTCTGAGAGCTGGT-1

using 17 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[17]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1350 = CGTTCTGAGATCTGCT-1

using 58 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[58]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1351 = CGTTCTGAGATGCCTT-1

using 118 reads

====================================================================================

graph has 126 edges initially, 8 edges after simplification

total ucounts = 24
nonsolo ucounts = 15[2^4, 3, 4^3, 5, 7, 9, 11, 12, 18, 24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1353 = CGTTCTGAGATGTCGG-1

using 50 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[50]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1356 = CGTTCTGAGCACCGTC-1

using 67 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[67]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1360 = CGTTCTGAGCGTAGTG-1

using 74 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[74]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1362 = CGTTCTGAGCTGGAAC-1

using 48 reads

====================================================================================

graph has 56 edges initially, 2 edges after simplification

total ucounts = 15
nonsolo ucounts = 6[2^2, 5, 8^2, 14]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1364 = CGTTCTGAGGCATTGG-1

using 80 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[24, 56]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1366 = CGTTCTGAGGGTTCCC-1

using 25 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1370 = CGTTCTGAGTAGCGGT-1

using 12196 reads

====================================================================================

graph has 6421 edges initially, 180 edges after simplification

total ucounts = 1007
nonsolo ucounts = 720[2^143, 3^76, 4^51, 5^45, 6^42, 7^33, 8^30, 9^27, 10^15, 11^25, 12^22, 13^23, 14^17, 15^18, 16^13, 17^20, 18^12, 19^10, 20^12, 21^7, 22^6, 23^8, 24^5, 25^4, 26^3, 27^4, 28, 29^3, 30^5, 31^4, 32^3, 33^3, 34^3, 35^2, 37^3, 38, 39^2, 40, 41^2, 42^3, 44, 46^2, 50^2, 51, 55, 66, 75, 85, 319, 353, 3970]
surviving nonsolo ucounts = 3[319, 353, 3970]
ids = [629, 859, 612]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1373 = CGTTCTGAGTCCGGTC-1

using 175 reads

====================================================================================

graph has 134 edges initially, 4 edges after simplification

total ucounts = 19
nonsolo ucounts = 12[2^4, 3, 12, 14, 16, 22, 28, 30, 35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1374 = CGTTCTGAGTCTCCTC-1

using 22 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1389 = CGTTCTGCAAGTCTGT-1

using 44 reads

====================================================================================

graph has 70 edges initially, 2 edges after simplification

total ucounts = 14
nonsolo ucounts = 7[2^3, 4^2, 5, 18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1391 = CGTTCTGCAATACGCT-1

using 115 reads

====================================================================================

graph has 85 edges initially, 6 edges after simplification

total ucounts = 13
nonsolo ucounts = 12[2^4, 3^3, 12^2, 13, 17, 43]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1395 = CGTTCTGCACAACTGT-1

using 11282 reads

====================================================================================

graph has 7170 edges initially, 144 edges after simplification

total ucounts = 1056
nonsolo ucounts = 776[2^130, 3^83, 4^92, 5^38, 6^42, 7^41, 8^40, 9^32, 10^25, 11^27, 12^26, 13^21, 14^30, 15^23, 16^14, 17^16, 18^18, 19^15, 20^11, 21^4, 22^3, 23^6, 24^4, 25, 26, 27^5, 28^2, 29^2, 30, 33, 34^3, 35^2, 36, 37, 38^2, 39^2, 44, 45, 48^2, 60, 63^2, 89, 113, 878, 2968]
surviving nonsolo ucounts = 2[63, 2968]
ids = [672, 785]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1397 = CGTTCTGCACAAGCCC-1

using 149 reads

====================================================================================

graph has 136 edges initially, 4 edges after simplification

total ucounts = 15
nonsolo ucounts = 10[2, 3, 4, 5, 8, 18, 20, 23, 28, 33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1399 = CGTTCTGCACACATGT-1

using 111 reads

====================================================================================

graph has 100 edges initially, 4 edges after simplification

total ucounts = 10
nonsolo ucounts = 4[3^2, 18, 81]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1406 = CGTTCTGCACCACCAG-1

using 80 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[80]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1409 = CGTTCTGCACCGATAT-1

using 195 reads

====================================================================================

graph has 152 edges initially, 10 edges after simplification

total ucounts = 15
nonsolo ucounts = 12[2^2, 3^2, 5, 6, 8, 9, 24^3, 82]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1411 = CGTTCTGCACCTCGGA-1

using 61 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[61]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1412 = CGTTCTGCACCTGGTG-1

using 55 reads

====================================================================================

graph has 70 edges initially, 2 edges after simplification

total ucounts = 12
nonsolo ucounts = 7[2^2, 3^2, 4, 13, 23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1413 = CGTTCTGCACGACTCG-1

using 31 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 4[2, 3^2, 17]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1418 = CGTTCTGCACTGAAGG-1

using 103 reads

====================================================================================

graph has 90 edges initially, 6 edges after simplification

total ucounts = 16
nonsolo ucounts = 13[2^5, 3, 5^3, 9, 13, 22, 28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1419 = CGTTCTGCACTGTGTA-1

using 40 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[40]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1420 = CGTTCTGCACTTGGAT-1

using 48 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[47]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1422 = CGTTCTGCAGACTCGC-1

using 87 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[87]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1423 = CGTTCTGCAGATAATG-1

using 56 reads

====================================================================================

graph has 86 edges initially, 2 edges after simplification

total ucounts = 13
nonsolo ucounts = 7[2^3, 3, 4^2, 33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1425 = CGTTCTGCAGCAGTTT-1

using 56 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[56]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1432 = CGTTCTGCAGGAATCG-1

using 32 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 5[2^3, 11^2]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1433 = CGTTCTGCAGGAATGC-1

using 66 reads

====================================================================================

graph has 64 edges initially, 4 edges after simplification

total ucounts = 21
nonsolo ucounts = 8[2^3, 3, 5, 12, 13, 14]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1441 = CGTTCTGCATGCAATC-1

using 43 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[43]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1442 = CGTTCTGCATGCATGT-1

using 18 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1444 = CGTTCTGCATGGGACA-1

using 32 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1447 = CGTTCTGGTAACGACG-1

using 28 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1448 = CGTTCTGGTAAGAGGA-1

using 45 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 16
nonsolo ucounts = 8[2^2, 3, 4, 5^3, 11]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1449 = CGTTCTGGTAAGGGCT-1

using 78 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[78]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1450 = CGTTCTGGTACAAGTA-1

using 33 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1451 = CGTTCTGGTACAGTGG-1

using 83 reads

====================================================================================

graph has 64 edges initially, 4 edges after simplification

total ucounts = 18
nonsolo ucounts = 11[2^3, 3^2, 6, 7^2, 12, 13, 19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 40.1452 = CGTTCTGGTACGACCC-1

using 57 reads

====================================================================================

graph has 51 edges initially, 4 edges after simplification

total ucounts = 13
nonsolo ucounts = 9[3^4, 5, 6, 8, 10, 12]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

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BARCODE 40.1453 = CGTTCTGGTACTTAGC-1

using 128 reads

====================================================================================

graph has 66 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[128]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

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BARCODE 40.1455 = CGTTCTGGTACTTGAC-1

using 26 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!
sorting bam, mem = 0.15
running samtools sort -l 8G -o /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_T_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk40-ucc6a7a72e6/files/contig_bam_sorted.bam /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_T_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk40-ucc6a7a72e6/files/contig_bam.bam

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90.794 seconds used processing barcodes, peak mem = 0.33
